LOCUS       DAZUMT010000001       100706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000001 DAZUMT010000000
VERSION     DAZUMT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.001"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..2167)
                     /locus_tag="ACG8FM_000001"
     CDS             complement(554..2167)
                     /locus_tag="ACG8FM_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5092702.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="ACG8FM_000002"
     CDS             complement(2308..3216)
                     /locus_tag="ACG8FM_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5092703.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3399..4523
                     /locus_tag="ACG8FM_000003"
     CDS             3399..4523
                     /locus_tag="ACG8FM_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5092704.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4548..4727
                     /locus_tag="ACG8FM_000004"
     CDS             4548..4727
                     /locus_tag="ACG8FM_000004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092705.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            4750..5667
                     /locus_tag="ACG8FM_000005"
     CDS             4750..5667
                     /locus_tag="ACG8FM_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5092706.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5660..6493
                     /locus_tag="ACG8FM_000006"
     CDS             5660..6493
                     /locus_tag="ACG8FM_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5092707.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6576..7694
                     /locus_tag="ACG8FM_000007"
     CDS             6576..7694
                     /locus_tag="ACG8FM_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5092708.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7715..9586
                     /locus_tag="ACG8FM_000008"
     CDS             7715..9586
                     /locus_tag="ACG8FM_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS5092709.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9580..10803
                     /locus_tag="ACG8FM_000009"
     CDS             9580..10803
                     /locus_tag="ACG8FM_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5092710.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10889..12823)
                     /locus_tag="ACG8FM_000010"
     CDS             complement(10889..12823)
                     /locus_tag="ACG8FM_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5092711.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13049..13705
                     /locus_tag="ACG8FM_000011"
     CDS             13049..13705
                     /locus_tag="ACG8FM_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5092712.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13779..14132)
                     /locus_tag="ACG8FM_000012"
     CDS             complement(13779..14132)
                     /locus_tag="ACG8FM_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5092713.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14324..15253
                     /locus_tag="ACG8FM_000013"
     CDS             14324..15253
                     /locus_tag="ACG8FM_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS5092714.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15264..16100
                     /locus_tag="ACG8FM_000014"
     CDS             15264..16100
                     /locus_tag="ACG8FM_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS5092715.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16348..17124
                     /locus_tag="ACG8FM_000015"
     CDS             16348..17124
                     /locus_tag="ACG8FM_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5092716.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17300..18043
                     /locus_tag="ACG8FM_000016"
     CDS             17300..18043
                     /locus_tag="ACG8FM_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5092717.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18036..19616
                     /locus_tag="ACG8FM_000017"
     CDS             18036..19616
                     /locus_tag="ACG8FM_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS5092718.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19867..20346)
                     /locus_tag="ACG8FM_000018"
     CDS             complement(19867..20346)
                     /locus_tag="ACG8FM_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5092719.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20362..21114)
                     /locus_tag="ACG8FM_000019"
     CDS             complement(20362..21114)
                     /locus_tag="ACG8FM_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5092720.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNNLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21231..21301
                     /locus_tag="ACG8FM_000020"
     tRNA            21231..21301
                     /locus_tag="ACG8FM_000020"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21263..21265,aa:Gly,seq:ccc)
     gene            21662..22387
                     /locus_tag="ACG8FM_000021"
     CDS             21662..22387
                     /locus_tag="ACG8FM_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5092721.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22642..22908
                     /locus_tag="ACG8FM_000022"
     CDS             22642..22908
                     /locus_tag="ACG8FM_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS5092722.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22905..23336
                     /gene="ruvX"
                     /locus_tag="ACG8FM_000023"
     CDS             22905..23336
                     /gene="ruvX"
                     /locus_tag="ACG8FM_000023"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS5092723.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23361..23666
                     /locus_tag="ACG8FM_000024"
     CDS             23361..23666
                     /locus_tag="ACG8FM_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS5092724.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23747..24892)
                     /locus_tag="ACG8FM_000025"
     CDS             complement(23747..24892)
                     /locus_tag="ACG8FM_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5092725.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24953..25600)
                     /locus_tag="ACG8FM_000026"
     CDS             complement(24953..25600)
                     /locus_tag="ACG8FM_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5092726.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25794..26087
                     /locus_tag="ACG8FM_000027"
     CDS             25794..26087
                     /locus_tag="ACG8FM_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092727.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26131..26442
                     /locus_tag="ACG8FM_000028"
     CDS             26131..26442
                     /locus_tag="ACG8FM_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092728.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26439..27063
                     /locus_tag="ACG8FM_000029"
                     /pseudo
     CDS             26439..27063
                     /locus_tag="ACG8FM_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27204..27362
                     /locus_tag="ACG8FM_000030"
     CDS             27204..27362
                     /locus_tag="ACG8FM_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092729.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27363..27725
                     /locus_tag="ACG8FM_000031"
     CDS             27363..27725
                     /locus_tag="ACG8FM_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092730.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27762..28610
                     /locus_tag="ACG8FM_000032"
     CDS             27762..28610
                     /locus_tag="ACG8FM_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS5092731.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28600..30075
                     /locus_tag="ACG8FM_000033"
     CDS             28600..30075
                     /locus_tag="ACG8FM_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS5092732.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30341..30748
                     /locus_tag="ACG8FM_000034"
     CDS             30341..30748
                     /locus_tag="ACG8FM_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS5092733.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30751..30966
                     /locus_tag="ACG8FM_000035"
     CDS             30751..30966
                     /locus_tag="ACG8FM_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092734.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30970..31350
                     /locus_tag="ACG8FM_000036"
     CDS             30970..31350
                     /locus_tag="ACG8FM_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5092735.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31484..31639
                     /locus_tag="ACG8FM_000037"
     CDS             31484..31639
                     /locus_tag="ACG8FM_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS5092736.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31708..32181
                     /locus_tag="ACG8FM_000038"
     CDS             31708..32181
                     /locus_tag="ACG8FM_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS5092737.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32178..33872
                     /locus_tag="ACG8FM_000039"
     CDS             32178..33872
                     /locus_tag="ACG8FM_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5092738.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33838..34023
                     /locus_tag="ACG8FM_000040"
     CDS             33838..34023
                     /locus_tag="ACG8FM_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092739.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34027..35202
                     /locus_tag="ACG8FM_000041"
     CDS             34027..35202
                     /locus_tag="ACG8FM_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5092740.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35195..36718
                     /locus_tag="ACG8FM_000042"
     CDS             35195..36718
                     /locus_tag="ACG8FM_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5092741.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36774..37058
                     /locus_tag="ACG8FM_000043"
     CDS             36774..37058
                     /locus_tag="ACG8FM_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS5092742.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37045..37380
                     /locus_tag="ACG8FM_000044"
     CDS             37045..37380
                     /locus_tag="ACG8FM_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5092743.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37675..38301
                     /locus_tag="ACG8FM_000045"
     CDS             37675..38301
                     /locus_tag="ACG8FM_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092744.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39043..39879
                     /locus_tag="ACG8FM_000046"
     CDS             39043..39879
                     /locus_tag="ACG8FM_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS5092745.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39943..40374
                     /locus_tag="ACG8FM_000047"
     CDS             39943..40374
                     /locus_tag="ACG8FM_000047"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5092746.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(40436..41758)
                     /locus_tag="ACG8FM_000048"
     CDS             complement(40436..41758)
                     /locus_tag="ACG8FM_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS5092747.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41912..43552)
                     /locus_tag="ACG8FM_000049"
     CDS             complement(41912..43552)
                     /locus_tag="ACG8FM_000049"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS5092748.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43885..44355
                     /locus_tag="ACG8FM_000050"
     CDS             43885..44355
                     /locus_tag="ACG8FM_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS5092749.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            44577..45680
                     /locus_tag="ACG8FM_000051"
     CDS             44577..45680
                     /locus_tag="ACG8FM_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS5092750.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45964..46644
                     /locus_tag="ACG8FM_000052"
     CDS             45964..46644
                     /locus_tag="ACG8FM_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5092751.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46734..47768
                     /locus_tag="ACG8FM_000053"
     CDS             46734..47768
                     /locus_tag="ACG8FM_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5092752.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47782..48117
                     /locus_tag="ACG8FM_000054"
     CDS             47782..48117
                     /locus_tag="ACG8FM_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092753.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48101..50581
                     /locus_tag="ACG8FM_000055"
     CDS             48101..50581
                     /locus_tag="ACG8FM_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092754.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            50637..52196
                     /locus_tag="ACG8FM_000056"
     CDS             50637..52196
                     /locus_tag="ACG8FM_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5092755.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52193..53557
                     /locus_tag="ACG8FM_000057"
     CDS             52193..53557
                     /locus_tag="ACG8FM_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5092756.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53728..54798
                     /locus_tag="ACG8FM_000058"
     CDS             53728..54798
                     /locus_tag="ACG8FM_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5092757.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54813..55481
                     /locus_tag="ACG8FM_000059"
     CDS             54813..55481
                     /locus_tag="ACG8FM_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5092758.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55501..56079
                     /locus_tag="ACG8FM_000060"
     CDS             55501..56079
                     /locus_tag="ACG8FM_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5092759.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56480..57391
                     /locus_tag="ACG8FM_000061"
     CDS             56480..57391
                     /locus_tag="ACG8FM_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5092760.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57479..58396)
                     /gene="rnhC"
                     /locus_tag="ACG8FM_000062"
     CDS             complement(57479..58396)
                     /gene="rnhC"
                     /locus_tag="ACG8FM_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS5092761.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58598..59035
                     /gene="zapA"
                     /locus_tag="ACG8FM_000063"
     CDS             58598..59035
                     /gene="zapA"
                     /locus_tag="ACG8FM_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS5092762.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59094..59642
                     /locus_tag="ACG8FM_000064"
     CDS             59094..59642
                     /locus_tag="ACG8FM_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS5092763.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            59757..62117
                     /locus_tag="ACG8FM_000065"
     CDS             59757..62117
                     /locus_tag="ACG8FM_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5092764.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62204..62518
                     /gene="trxA"
                     /locus_tag="ACG8FM_000066"
     CDS             62204..62518
                     /gene="trxA"
                     /locus_tag="ACG8FM_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS5092765.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62700..64478
                     /gene="uvrC"
                     /locus_tag="ACG8FM_000067"
     CDS             62700..64478
                     /gene="uvrC"
                     /locus_tag="ACG8FM_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS5092766.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64623..65522
                     /locus_tag="ACG8FM_000068"
     CDS             64623..65522
                     /locus_tag="ACG8FM_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5092767.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65515..66777
                     /locus_tag="ACG8FM_000069"
     CDS             65515..66777
                     /locus_tag="ACG8FM_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5092768.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            66938..68836
                     /locus_tag="ACG8FM_000070"
     CDS             66938..68836
                     /locus_tag="ACG8FM_000070"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS5092769.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(69166..69885)
                     /locus_tag="ACG8FM_000071"
     CDS             complement(69166..69885)
                     /locus_tag="ACG8FM_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS5092770.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    70075..70122
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            70222..70554
                     /locus_tag="ACG8FM_000072"
     CDS             70222..70554
                     /locus_tag="ACG8FM_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092771.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70541..72544
                     /locus_tag="ACG8FM_000073"
     CDS             70541..72544
                     /locus_tag="ACG8FM_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS5092772.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72563..73033
                     /locus_tag="ACG8FM_000074"
     CDS             72563..73033
                     /locus_tag="ACG8FM_000074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS5092773.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73054..73638
                     /locus_tag="ACG8FM_000075"
     CDS             73054..73638
                     /locus_tag="ACG8FM_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFS5092774.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73653..74639
                     /locus_tag="ACG8FM_000076"
     CDS             73653..74639
                     /locus_tag="ACG8FM_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS5092775.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            74629..74940
                     /locus_tag="ACG8FM_000077"
     CDS             74629..74940
                     /locus_tag="ACG8FM_000077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS5092776.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            74962..76743
                     /locus_tag="ACG8FM_000078"
     CDS             74962..76743
                     /locus_tag="ACG8FM_000078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS5092777.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            76736..78112
                     /locus_tag="ACG8FM_000079"
     CDS             76736..78112
                     /locus_tag="ACG8FM_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS5092778.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78119..78754
                     /locus_tag="ACG8FM_000080"
     CDS             78119..78754
                     /locus_tag="ACG8FM_000080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS5092779.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            78784..80139
                     /locus_tag="ACG8FM_000081"
     CDS             78784..80139
                     /locus_tag="ACG8FM_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5092780.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80331..81725)
                     /locus_tag="ACG8FM_000082"
     CDS             complement(80331..81725)
                     /locus_tag="ACG8FM_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5092781.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            81817..82788
                     /locus_tag="ACG8FM_000083"
     CDS             81817..82788
                     /locus_tag="ACG8FM_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS5092782.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            82785..83567
                     /locus_tag="ACG8FM_000084"
     CDS             82785..83567
                     /locus_tag="ACG8FM_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS5092783.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(83736..84107)
                     /locus_tag="ACG8FM_000085"
     CDS             complement(83736..84107)
                     /locus_tag="ACG8FM_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5092784.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84241..84684
                     /locus_tag="ACG8FM_000086"
     CDS             84241..84684
                     /locus_tag="ACG8FM_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5092785.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(84727..85536)
                     /gene="yidA"
                     /locus_tag="ACG8FM_000087"
     CDS             complement(84727..85536)
                     /gene="yidA"
                     /locus_tag="ACG8FM_000087"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS5092786.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(85555..86922)
                     /locus_tag="ACG8FM_000088"
     CDS             complement(85555..86922)
                     /locus_tag="ACG8FM_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5092787.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(86906..87736)
                     /locus_tag="ACG8FM_000089"
     CDS             complement(86906..87736)
                     /locus_tag="ACG8FM_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS5092788.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            87899..88336
                     /locus_tag="ACG8FM_000090"
     CDS             87899..88336
                     /locus_tag="ACG8FM_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS5092789.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88412..89032
                     /gene="rpoE"
                     /locus_tag="ACG8FM_000091"
     CDS             88412..89032
                     /gene="rpoE"
                     /locus_tag="ACG8FM_000091"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS5092790.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89221..89727
                     /locus_tag="ACG8FM_000092"
     CDS             89221..89727
                     /locus_tag="ACG8FM_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5092791.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            89744..90643
                     /locus_tag="ACG8FM_000093"
     CDS             89744..90643
                     /locus_tag="ACG8FM_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS5092792.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            90730..91614
                     /locus_tag="ACG8FM_000094"
     CDS             90730..91614
                     /locus_tag="ACG8FM_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5092793.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(91806..91877)
                     /locus_tag="ACG8FM_000095"
     tRNA            complement(91806..91877)
                     /locus_tag="ACG8FM_000095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91842..91844),aa:Arg,seq:ccg)
     gene            91937..93271
                     /gene="rpoN"
                     /locus_tag="ACG8FM_000096"
     CDS             91937..93271
                     /gene="rpoN"
                     /locus_tag="ACG8FM_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS5092794.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            93420..94511
                     /locus_tag="ACG8FM_000097"
     CDS             93420..94511
                     /locus_tag="ACG8FM_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS5092795.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            94552..95553
                     /gene="gap"
                     /locus_tag="ACG8FM_000098"
     CDS             94552..95553
                     /gene="gap"
                     /locus_tag="ACG8FM_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5092796.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            95663..96853
                     /gene="pgk"
                     /locus_tag="ACG8FM_000099"
     CDS             95663..96853
                     /gene="pgk"
                     /locus_tag="ACG8FM_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS5092797.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            96957..97712
                     /gene="tpiA"
                     /locus_tag="ACG8FM_000100"
     CDS             96957..97712
                     /gene="tpiA"
                     /locus_tag="ACG8FM_000100"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS5092798.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            97828..99126
                     /gene="eno"
                     /locus_tag="ACG8FM_000101"
     CDS             97828..99126
                     /gene="eno"
                     /locus_tag="ACG8FM_000101"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS5092799.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            99525..100664
                     /locus_tag="ACG8FM_000102"
     CDS             99525..100664
                     /locus_tag="ACG8FM_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS5092800.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        33406 a        17204 c        20781 g        29315 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     3301 aaatattttt ttatataaat atttgtgtta ctaattccgt taatatacat aagtgaaagc
     3361 gttttatata agaacttgaa aagaaagaag ggatagacat gaaaaaaaga tacaaattgg
     3421 tgatagcttt gctaaacatt accctattat tcagcttaag tgcatgtgga aaaactaacg
     3481 aagaagtaac agcaaacatc gcagatccaa tgataaaaaa agccactaca ttagaagtat
     3541 atgtcgcaaa cggcaaactg acaaactatg ttgaaaatgc tgcaaaaatc tataaccgca
     3601 aaactggagc aaatattgac ttaaaaattg tgaatgtagc ttcgggaaca gcaacagtac
     3661 aaatgatcac cccaaaattg gtaacacacg aagatatgcc ggatattatc tccatggcag
     3721 acacttcagc agcaggtgtg ctggaaaaat ttcaagatgc tttttatagt gctgatgaat
     3781 atggatttta tgacaaatat ggcgatcagt tttatgatca aaaattgaat attttgcgtc
     3841 aacaaacgag taatgaaacg attattcctt gggctagtga ttttacccca gcactttgtt
     3901 attatcaacc aaaaaaattt gctgaaatag gtgtagattt tgaagaaatc agttcttggg
     3961 aagaatatat ccaggtggca aaagcattaa aagaaaaaac tggtagtttt ggaattgcct
     4021 tgccagaatc tggggaccaa gaattgttta tcaacatgct agcccagcag aatcaatctt
     4081 tgcttgatga agaaggaaat atccgtctga atactaaaga agcgaaacat gctgcaaagt
     4141 taatcaaaga aatgatcgac tcagatattg tacatttcta cggtgcacaa gatgctgaga
     4201 aagcatttca agaatctgca atgtttgttg ctggcggctg gtatgctacg aatatgtctt
     4261 tgaattttcc tgatgcatct gacaaatggc gcatggctcc actgattcct ttttcaaaag
     4321 aaaatccagg aaaaggtgca gtatcaggag gatcaagttt ttatgtccct aaagatgcaa
     4381 aaaatccaca agtggcacaa caatttctta catttatgct gacagatgaa gaatgcttag
     4441 ccaacgcact ggagctgggc gtggctacat ctaactcatt ggcctatcaa acagaagcag
     4501 ctgagaaaaa gtttgactac taaggaggac aaaaatacta tgaactattg aataaaatca
     4561 atgaaaatac atcaaatact gttttaccag ccacttattc agatgctacg tcttatttga
     4621 ctaccgctgc atataactat tggcaaacag gtgattttga aaattcatat ctaaaagaag
     4681 cagaaaattt tgctcataag tacggcgtaa aattaactgg tgattaaaaa atattaggag
     4741 gacttaacga tgatgcaatc ttcgttacca agcaagacaa taaagaacag caagaaaaaa
     4801 gcaacagata cgtctcataa aaaaagtgct tatggattgg tcacaccttt tgttattttc
     4861 tttattgtga cgattttgat tcccatcatt atctcgtttg cttttagttt tatgaaaatt
     4921 ggctactcat ttgaatttgt tggatttgac aattatctag ctatcttccg agatccattg
     4981 tttattaaat cttacacgaa tgttttgatt atcatggcag gatcaattcc aataacgatg
     5041 ctattagcta tttactttgc tgttttattg aattcagcac agttaaaagg aaaaggattt
     5101 tttagaactg tctattatat tccaaccatt acatcaacgg tggctgtagc ttccgtattc
     5161 atgacttttt tcaatccgac tgggttattc aatggatttt tgaatgcgct aggaattaac
     5221 ggtgtacagt ggttaacaaa tcctttttgg atacgaatat caatgatcat cacgatgatt
     5281 tggcttagtg taggatataa cactatttta tttttagctg gtcttcaagg gatttctaaa
     5341 gagatatacg aaagtgctga aatagatgga gcatcgaaat ttcggcaatt ctttttatta
     5401 acgatcccca tgctaaagcc aatcatttta atggccacag ttttagccac tatcaatggc
     5461 ttgggtaatt tcaatatccc aaatatattc tttgggactt ccaatggacc agaaaattca
     5521 gctttggtcg taggagtcaa tttatataaa acaagttttg aaatggttga ttttggaaaa
     5581 gcctctgcta ttgcctggac gatggtgttt gtatctgtta tattatcggc tattcaattt
     5641 aaattcggag gtgataaaaa tgactaaaca aaagatacaa agcagaattt ttacgtacgt
     5701 cattattgtg tttggtgcct taatattttt gatgccatat ttatatatga ttgtagcttc
     5761 aacgcaaagt aatcaagaaa ttttaagtgg cggaatcaat ttcaagtttg gggatatgct
     5821 attaaaaaac tggcaagcca tcatggcgag atataattat cccttgatta tttggaattc
     5881 gattgttatt gctgtaatca gtacagtttt agctacagcc agttctacaa tggcgggtta
     5941 tgcattagca aagtatcgtt ttatggggaa taaactgttg ttcaatacag ttatgctatc
     6001 taggatggtg ccttttttta ccacattgat tcctttattt tacattatga gtcgtttagg
     6061 attatcgaat acttatgcag gtgtgatcat cccttcgatt gccagtacaa cttcagtgtt
     6121 tatgatgcga caatatagca gtcaatttcc tacagcactg atggaggctg ccagaatcga
     6181 tggtgccaat gagtgggtga tttttacgaa aatcgctgta ccgattttat cacctacgat
     6241 tattactagt ggtttactta tctttatggg aagttggaat caatatctat ttccattagt
     6301 catgctttca gataaagata aatatacggt tcccttaatt atcaaaaata tgacggtttc
     6361 tagtacaggt gagccaatta attatggcgc aattatgcta attttagcaa cttcagtgat
     6421 cccaatggtc ttgatttatg tgtgggcgca gtctaaattt aaggagaatg acattggagc
     6481 tgcaaataag taaaaaaaat aacgaatgtt aaccatctag ttcgttctag gagcaactcc
     6541 acattcataa aaattaaaag aatggcggga tatccatgaa tgaacagacg aaggaatggt
     6601 caagaaaaat tttgaatgta gcttctgaaa agatagatgc ggaattagac agaatgggga
     6661 ataaaattcc atatatttct tacaacggaa aatatacaga cgtaataaag gacagaggaa
     6721 tcgactggtg gaccaatggt ttttggagtg gtattttatg gcagctcttt cacaagacaa
     6781 aacgagaaaa gttcaaagag gcagcaatac aacaagaaca attattagaa tctgccctgt
     6841 accatttttc agacgttcat catgatgtag gttttttatt cttaccatcg gctattgcac
     6901 attatcaaca gtctcaaaac aaacatagtc gacaactagg gttacatgca gctacggtac
     6961 ttgctggacg ctataatatt tcagggaact atttagtagc atggaatgac cataaacctg
     7021 gttggatgat tattgatagt atgatgaatt taccattact gtattgggct tcgagggaaa
     7081 ctggtgatcc gaggttctct aaaattgctc ggcagcatgc agatacaaca gctaagtatc
     7141 tagtgaggaa agatggatca accgggcata tcgcagaatt tgatgcagaa tcagggaaat
     7201 atattcggtt acttggcggg caaggataca atgagaattc ttgttggagc agaggaaatg
     7261 catgggcttt atatggattc gcgttaagtt atcgttacac aaaaaatccg tactatctag
     7321 ctatagcaga aaagatagca gtaaatttta ttcagaaaat cgaaaaaact ggttatattc
     7381 ctctcattga ttttgctgta aaagattcga atattgatac cagtgctgga gtatgcgctg
     7441 catgcggtct tctggaatta gcaggatatg tttcctctgt acgatcaaaa atgtatcatg
     7501 aagctgctat caatatactc caaaagacag aaaagcacta tgcaaattgg aatatggatg
     7561 aagatgggat cattggaggc ggagcagaag cataccatag agaactgact tataatgtgc
     7621 cactgatcta ttctgattat ttttttattg aaggcttact tcgattagaa cagtcggaat
     7681 tagctatttg gtagtaaaaa gaggagaaaa tacaatgaaa catccagtac cactattaag
     7741 gaccagacag gattactttg acttatttaa aaagataaat caaccactcc agccgtttta
     7801 tcgagcaagt ggcgcacaaa ttgattatat acatcaaggg gtaggatatg gaaataaaat
     7861 tgcaggaatg gaaggattct ctcgggtttt atggggagcg gggcccgcaa ttgaccaatt
     7921 agatgaacga tggttgaaat taattctatc aggaataaaa gctggaacag atccttccca
     7981 tcccgattat tggggagaaa tacatgaaag agaccagcgt atggtagaaa tgcccgcaat
     8041 gttattagcg ttatatcatc atgaacaatt attatggaaa aaattagata caggagaaaa
     8101 aagccagatt attcattggt tctcgcaaat ttttgaatat gaatgtgcag atggtaattg
     8161 gcaatttttt aagataattg tcggaacaat tatccaaaaa tttggttatc cagttaaaga
     8221 atcagcactg aaagaagcat ttgataatat agaaaattgt tatcttcaaa atggctggta
     8281 tcgagacagt tcaagaggtc gccaagatta ttataatcct tttgcatttc attattatgg
     8341 tttgatttat agcgtactag aaccagaaaa gcccattagc cagatatata agaaccgagc
     8401 tattgatttt tctagagact atattcattt ttttgcggaa gatggtgcaa atgtgccttt
     8461 tggtcgaagc atgatttatc gttttgcagt ttcaagcttt tggtcagcta tgttgtgggc
     8521 cgatcttttg ccttttaaat taggtgaaat caaggggtta gtcaatcgga atataagatg
     8581 gtggatgcaa aaagaaatat ttgatgaaaa aggtatcctg aatattggtt atacctaccc
     8641 tcaattgaca ttgactgaac cctataatag tacgttatct ccattatggc tgaataagat
     8701 ttttttattg ttggcgctac ctgaagacca tgagtattgg acggctaaag aaatgcctat
     8761 gcctattcgt gaaggaacta aacttctttc agaagccaat ttgctcatac aacatgataa
     8821 tggacataca ttttttttga atgcgggaca attagggcca aatttccata cattgaccaa
     8881 tgaaaaatat ttaaaatttg cttattctag tcaatttggt ttttcgatac cgcgagcgaa
     8941 tcaattgaaa gcagaaatgg caatggattc aatgctaggg attcagcgag cggacacata
     9001 tatcacgacc agttttaaga atgagagcat caaaacctat ggacagttca tcgttcgaaa
     9061 taatgtacga gacattgtat gtaagaaaga ggtacttgcc tctacatggg aacctacttt
     9121 acaggcaaaa atcagaactt ggcttattcc ttttgaaggc tggcagattc gcgtacacaa
     9181 agtagaatta gatacagaat atgttctata tgagacaggg tttgctattg aatgttctcc
     9241 tgagaaagaa ggaataatca tagaagaaga caaggaaaac tatagggtga gtgaagctgg
     9301 cttttcaggt attatctgtt taagtgacga tactattaaa cgaaaatcca cagctattat
     9361 gggtttgccc aatacaaact tgatgacatg ggaaaacact tttattcctg gattagaggg
     9421 tacatttgga aaaggaaccc attggctcgg aacaggagtg gttgctcatc aagatcggga
     9481 ttatagttta gaagtatgga acaatcgtcc caaaattgat tttgatggga caaccgggct
     9541 aactatccaa aataaaaaca ataaaaaaag actaaaatta tgctaaaggc tggtggggaa
     9601 atgcttgata aagtgatgtt tgaagagata atacatccag aaaaatggat aaatgaatgt
     9661 tataaagaat taatggaaaa agaatggaat aatgagatcg agcgttgtcg cagacacacg
     9721 gagaggttaa tcaaacgtat cagtaacagt cagtatcaat tcccaagcga gtgtcctata
     9781 aaaggacgtt atccattaag agacaatatt ggttggacag aaggattttg gacaggaatt
     9841 aattggttgt tatttgagaa gacgggtgat cctttatttc aacaaacagc tgaaaaagca
     9901 gatatagatt ttatccacag gattgactgt caaattatca ctaatcatca cgatcttggt
     9961 tttttgtatt ctttatcaac cgtagcagat tataaaataa cgggaaatcg aaaaatgaag
    10021 gaagcttcaa ttgatgctgc agaaacatta cttcagcgtt tttgggagaa ggcaggagtt
    10081 atacaagctt ggggagatcc tagtgatcct tcacaacaag gtagaatgat catggattgt
    10141 tgcatgaatc ttccattatt gtattgggcg agtgaagaaa ctggaaatca aaaatataaa
    10201 aaagctgctc aggctcatat tcaaaatgca gcagattatt tagtaagaga agatggttca
    10261 acttatcata cattttatat ggatgtcaaa gatggaaaac caagatatgg aagtacgcat
    10321 caaggttata gtgataattc ctgttgggct agaggccaag catgggggat ttacggtttt
    10381 gcattaagtt atcgttattt gaaagaacaa aaatattttg atttaagcaa aatattagcc
    10441 aattatttta tgaatcggtt gccagaagat accatttgtt attgggattt gatctttgat
    10501 gattctagca gagaagaaaa agatacatct agtatggtga tcatggtttg tggattatta
    10561 gaattaatag aatcaggtct attagacaaa acagaaaaga acttatacca gtctgctgtt
    10621 tggaaaatta tgcgacaatt gaaagaaaga tatgctacaa cggaagaaga agatggcttt
    10681 ttaacacagt ctgtttataa taagcccaaa aataatgggg taaatacacc ttgtatttgg
    10741 ggtgattact attatttaga agctttagtg agaatgacta gtaattggtc ttcttattgg
    10801 tgaaaaaggt aaatatggaa tttttgtttg ataaaataaa aaaagaaaaa ccaaaacagc
    10861 atgaatggtt tctgttttgg ttttttcgtt aattttcata ttcttctaat tccaagctta
    10921 gttcttccca ttccgtcaat tttgtttctt gatcttgtct agcagcttct aaatcttggt
    10981 tcagctcatt gagttttacg tgatcttcca ggttttctgg cgcgaccatt tcttgttcca
    11041 actgatcgat ccattcatcg atccgcgcca tttcttcttc aacctgagta atttttcgtt
    11101 gaaggctacg gataagcttt tgctgttctt tgctttggta ataatcagat ttgttcgtgg
    11161 gagcaggagc agattctggc tcatcttgtg ccaacaattc ggcgatttct tcttcttcct
    11221 gctttttctc taaataataa tcatagtctc ctagatagac tttgctgcca ttttcagaga
    11281 gttcgacgac ttttgtcgcg atccgattga tgaaatatcg gtcatgggaa acaaaaagca
    11341 gtgtgccgtc ataatcgatc aatgcatttt ctaatacttc tttgttgtca atatctaaat
    11401 ggtttgttgg ttcatccaaa atcaaaaaat tttctttgtt catcgctaac ttggctaaag
    11461 caacccgtgc tttttcaccg ccacttagca gagggattgg tttttctaca tcttcgccag
    11521 aaaataagaa agcacctaaa gcacttcgga tctctttttc tggagtggta ggatgctcat
    11581 cccacaattc tgctaggatc gtcttgtttg tatgcagatc agcttgacct tgatcgtaat
    11641 agccgatggt gacatttgtc cctagcgttt cttttccacg gataaaaggg atctggtgaa
    11701 tgattgattt cagcaacgtc gatttcccga taccattcgg acctactaga gcaatcgctt
    11761 cttgacgacg gatatctaga ttgattggtt cagataaggt gacattatca tatccgatag
    11821 cggcatcttc gatttgcaag actacatttc cagaaggctt ttctatactg aatagaaagt
    11881 ttgcttgttt ttcatcccct tgaggacgat ccagccggtc cattttttct aatgtctttc
    11941 tgcggctttg cgctcgtttt gtggtagagg cgcgaacaag gttccgagca acgaagtctt
    12001 ctaatttatg gatttctgtt tgctgctttt cataagcctt ccattcactt gctaactgtt
    12061 ctgctttcaa atctagatat ttcgaatagt tacctttgta atggcgcatt tttcttctgc
    12121 ttaattcata gacttcattg actactttat ctaaaaaata acggtcatgg gaaacaatca
    12181 ataatgcacc agtatagctt tgcagataat tctcaagcca ggaaagtgtc tcgatatcta
    12241 aatggttcgt tggttcatct agaatcaaaa tgtcagggtg ttgcaaaagc atgcgagcta
    12301 aagctaaacg tgtgcgctgt ccgcctgata aagtatcgat tttctgatca taaaatgaag
    12361 aatcgaattt aaacccatgc aatacggaac gaatttcatt ttcataacca tagccgtttc
    12421 gatctgaaaa atcgtgttgc agccggtcgt attcttttaa ataagaacta taagcaggtg
    12481 tgtctggcgt accttcgcta atgaaaatct ctagctcgcg catccgctct tccatttgtc
    12541 taacttgttc aaaagctttc agcatctcgt tccaaactgt ttcattggaa tcaagtcctg
    12601 tgtcctgagc gaggtatccg agagtcgctg ttttattttt agcgatcttt ccttcatcag
    12661 gtgcttcaat tccagcaatg atctttaata aagtcgattt tccagcgcca tttctgccaa
    12721 ctaaagcgac acgagcgcct gtactgattt ctaaatgtat gttttcaaat aagacttctg
    12781 caccaaaatg gcgagcaatc tgattggctt gtaataatat catggaatcc tcttttctac
    12841 tttgtgaatt ctcctcaaat aaaaaactat ttttcaatta gtagtctacc ataaattcca
    12901 ttatactgcg agatttgcag aaatgataaa taaatgaata aagaataaaa tttcacaata
    12961 tctgagcaat ggaattgatt ttttcctata aatactgcta tgatggagaa atcaaagatg
    13021 taattagaca atacgtggag gtcatttcgt gaaagatcat attattccaa aggcgacagc
    13081 taaaagactg cctttgtatt atcgctattt aaggatttta aataatgctg gaaagaaaaa
    13141 agtgtcttca actgaattaa gtgaagccgt tcaagtagat agtgcaacga tccgtcgaga
    13201 tttttcttat tttggcgaat taggaaaacg tgggtatggt tatgatgtag aagatcttat
    13261 gcactttttt gcaaagacat taaacgacga tcaattgaca aatgttgcgc tgatcggtgt
    13321 agggaacttg ggaagtgctt tactaaaata caaattccat caaagtaata gcatacgtat
    13381 cagttgtgct tttgatgtga atgaggaaat cgttgggcgc atcgtagacg gtatccccgt
    13441 ttatccaatg gaagatatgt cagaacaaat caagatccaa caaatcgaag tagcaatttt
    13501 aacgataccc gctgaaagag cacaagaagt agtcaatcag ttaacggaag caggaatcaa
    13561 aggaatcttg aactttaccg ctgctcgatt gaatgctccg tcagatgtat tgatccaaaa
    13621 tgttgatttg acgaatgaat tgcaaacctt gatctatttc ttgcattctg acgcaaatgc
    13681 gcattccgaa aatacagcag aataaaaata aaaagcgatt gaacaaatga aggcaaaact
    13741 ttcacttgtt caatcgcttt tttctgtttg gacaacgatt attttgttcg gaagatttct
    13801 tcataagaaa gggaatcatc ttctgctgtc agttcatcta gtagataacg gattttttta
    13861 aagtcgatga accgtgcttg ccgaaagact tcttttcctt gatgaaaaat caggacaact
    13921 ggcgcagtca gcacttcaaa tttactagct acttctggtg tttcaaatgc atccaaatga
    13981 agtgccggaa tatcataatg tgtgagaagt tcctctagtc gtggacgaac agcgtgacaa
    14041 acactgcaat taggcatcga aagataaatg acggcaacgg atgactgttg cagtatgtct
    14101 tgaacttcag tcaagttagt ggtttgtttc atttttattc tcccttttat gatagaagtg
    14161 atatgtatga gaccttaatt taatgggaat aacagaaaaa gtcaattgaa gtgctttggt
    14221 aaaaaataag agttgtgtaa aaatttacag agcaacttgt tttttttgaa taaaagcgta
    14281 tatttgcaaa gcgaatgaac tagaaataaa aaggaggaaa ctcatgtttt cttttattcc
    14341 aaattcggtt ttgcagatga gtacgatttt cttatcgatc gttattgaag cattaccttt
    14401 cgtattatta ggctgtctta tctcaggcgc cttacaagta tttttgaccc cagaacgtgt
    14461 gaagagatgg cttcctgaaa atcgtatctt ctctattttt actggttcga tcttaggatt
    14521 tttctttcct tcatgtgaat gcggcatcgt accgatcgtt catcaatttg ttaaaaaagg
    14581 cgtaccagtt cacacagcgt ttgcttttat gctgactgct ccgatcatca atccaatcgt
    14641 gattttttcc acttttatcg catttggcaa ttcttggcag atggcaagct ggcgggtgat
    14701 aggcagcttt tttgttgccc ttgtgattgg tgtctggctt gcctattttc aaaaagacgc
    14761 tgtattgaaa gaaaaaatca cgtctaaagt agcagaaaat caccatcatc atgtccatga
    14821 agaagaacaa agtgagaaga atgaaccaaa aaatcagctg atccatcaaa ctgggcatgt
    14881 gttgacacat tcaatcgacg aattttttga tacaggtcgt tatttgatca ttggcggatt
    14941 aatcgcagct gcgatgcaga cttatttacc aactagaatt atgctaacgc tcggctcaac
    15001 aaagatttta gccattatca tcatgctgtt attggccttt acgatgtctc tttgctcaga
    15061 agcagatgcg tttataggat cttctttgtt aagtctgttc ggcacagcac ctgtagctgc
    15121 ctttttggtc tttggcccaa tggtcgatat caaaaatcta ctgatgatga aacgatattt
    15181 tacaggacgc tttattttat cgctgatcgg actgattgcc cttagcgtta ttggattcac
    15241 attatttatt taggagggaa aatatgctgc ggtttttgat tttatctggg tatttcgaat
    15301 tgatgatgta cttgcaagtt tctggcaaac tcgatcaata tatcaatgtc cattatcgct
    15361 acttagctat tctttcaatg attttatctg ctcttttggc tttagttcag ctgtatttgt
    15421 gggtcaaaaa cggaaaagca gaagaaaagg cacacgagca cgatcatgac catgggttgt
    15481 caaaacctta tcaacgtgga atcgcttatg tactgctagc tttgccagta atcgtgggga
    15541 cgttgtttcc aacagttagt ctcgacacta cgatcgtaga agccaaagga tttaatttcc
    15601 cagtaagtaa agaatcagta ggggatccgg agatgcagac acaatactta aagccggata
    15661 ccagtattta tttcaacaaa tcagactact tagaccaaat gaatgagttg aaagaaaagt
    15721 atggggataa gcaaactatc aagatcacag atgaaaacta tttagaagtt atggagctaa
    15781 tttataatta ccctagcgaa ttcattggca aaaaaatcag ctatgaagga tttgtttacc
    15841 aaacaccgaa cgatagcaat acagatatct tcttgttccg attcggcatt atccattgtg
    15901 tggcagattc aggagtcttt ggtttgttga cgcatatgcc ggaaggagta acggttaaaa
    15961 atgatgaatg gtataaaata gaaggaacca tccaatcaga ctattatcaa ccattcaagc
    16021 gtgaaatccc atctgtagtt gttacaaacg ctgagaaagt ctctgctccg aaaaatcagt
    16081 atgtgtatcg ttctttttaa aagaacaaaa taaataaaca tagctcagac agctgacacg
    16141 caaggacgtc ggttgtctgg ctttttttgc atgatgtatt catttgtttg atatttattt
    16201 tagaagcagc atcctgaaag tatttgatat cggtttcaaa ggtgttggaa aagattgtga
    16261 agagaattac aatttattcc taacatgtat gttgaaaagt tgtatgaaat gattttgata
    16321 gtaaaatatc gagtggaggt actttttatg gtaacgcgta tatttatttt ggatgatagt
    16381 ttggtttttg aaaaaatgct agtagaaatt ttggaagaat ccagaatttt attaagtcct
    16441 tggaaatttg agatcgttca gggattgaac gttatacaat ttgttgaatt cattaaaaat
    16501 aatgaaatct attcctcaga tatttttttc ctagacatcg atttgaacgc ctattatagc
    16561 ggtatcgaac taggcgaaat gcttagagaa aagaatgaaa agtgtaaaat tgtttatcta
    16621 acgtctcatc cgaataaagc aataactgtt attaatcgaa agattacgcc tagtggttat
    16681 ttgataaaag aattcactta tgaaaaaaat aaaaaaagca tccaaagtta cttattgaaa
    16741 gaaaaagaaa actatcctgt tcaacatgct acgaaaaaaa ggtggacaac tgtccgttat
    16801 ggtaatagcg atcactatat ccattacgat gaaatccttt atctaatgag catgccaggt
    16861 tataaaaata aaataggcct agtattgaaa gatgaagaga ttctcgtaaa cggcacaatt
    16921 aaaaaatata aaaaatcact cgaatatgag tatttatgta aagatttaaa agcttatatc
    16981 attaatactg aaaacattac atctattagc agagtaaacg aaatagtaac atttgcaaat
    17041 ggagaacaac tagagatggg aaagagaagt attgataaac taaagaattt tatgcaagaa
    17101 aagatagaaa atgaatcatt ttaaagaaaa caaagactca tatcttacct gttagaagag
    17161 tgttttcctg tagaagagaa caacccaagt agcagcctag tcgtttctat ccttttttgt
    17221 tatgcactct ttttgacatt tactccacca ataacgaagg gtacaataaa aagtaataac
    17281 aaacaaaagg agtacagaaa tgattgaatt catcaatgta agtaaaagtt acgggaaaaa
    17341 acaagcatta aaagacttgt ctctctcgat aagacaaggg gaaatttttg gatttttagg
    17401 acataatggt gccggaaaat caactacgat taaaagttta gttagtatta ttgaaccatc
    17461 atcgggaacg attttagtag atggaatgaa attgacggaa aatcgattga gtatcaaaca
    17521 aaaaatcggt tatgtgcctg attccccaga tattttttta caattgacgg ctggagaata
    17581 ttgggacttg atttcagctg cctatgaatt agacacacaa aaaaaagaaa agcgtttagc
    17641 agaattgaca gcgctttttg atatgtatag tcatcaaaat gaaacaatcg caagtttttc
    17701 acatggaatg cgtcaaaaaa cgattttgat cggcacgttg ctgccagatc cagatatttg
    17761 ggtattggat gaacctttgc aaggacttga tcctcaagca gcatttgatt tgaaagaaat
    17821 gatgaaggct catgccgcaa aaggaaaaac agtcattttt tctacacatg tattggatac
    17881 cgcacaacag ttatgcgata aactggctat tttgaagaaa ggtgaattga tttatcaggg
    17941 ggcagttagt gaattattga atggctcacc tgatgaaacg ctagaaaaga tttatttgaa
    18001 aatggctgga aggcaagcga caggagatgg accgtatgta taagcgacaa ttattagaat
    18061 taattcgagt taatctttta tatgttaatc cacagctcac gaataaagca agagagaaag
    18121 gaaaaaaggg aaagcgatta actatctatc ttttgtctca aaatcttttt tcagggctga
    18181 tttttttact gatttacgga tttgctatgt ttgctatgaa ctttgttaaa ttgccaggtt
    18241 tctttactta ttacgtggca ctttttggta tgttaggttt aactcaaagt atatcagtca
    18301 tttataatat cttttttgaa ggaaatgacc tgcaagtatt tttgcctctg ccttttggac
    18361 aagaacaggt tttttcagca aagattttgg tagttgcttt gaccatcatt ccttttgttt
    18421 ttccgcttct agtcatgttt attttaaccg gatggcgagc tgatctaggg attttgctta
    18481 ctatcattat ctcaatcctt ttgttttctc tatttttgat tattcttttt tgtctggcta
    18541 gtttgattgt ttttggtttg gctcgaacag cagtgtttaa aaaacataga aaaacagttt
    18601 ctagtatctt gcttggcagc tcgatgctga tcgcatttgc tggaatcatg tggatgaatc
    18661 atcaaacggg gactattgaa aatgagttaa cagatcggca tccgatttcg atattattgc
    18721 cattctatga tgctgccagt cagccacttt ctcaacaagg tttattgggt tggggaatac
    18781 tagctatcac ggctctggga tttttccttg ctgtaaaaca gctgatagta ccaaagctta
    18841 cagagcaact gactgattca aacagtgagt tcaaaataaa aagaacccat aaaaagaacc
    18901 aaaatgtgca tcagctttta tttagttaca atgctcaatt agtaaaagaa cctaatttga
    18961 ttatgcaagt tctttcttct tccttgttaa ctccaatcat ttttattctt gcttttgccc
    19021 taggaggcga aataaaatta actgatttag ataataagtt aatcggtgtt gttttcctgg
    19081 tagggatcgc attggctttc ttaacagtta atcagacttc ttttatcagc aatcttattt
    19141 ctttggatca agaaaatttt ctgtttatcc gttctttgcc aatctctatg aaccaatatt
    19201 taaaagaaaa gttcagattc ggatggatat tgcagagttt tttaactggt gggatcgcat
    19261 tattaagcgg actgtctttt cagctgcctt tgttttttat ccctagcttg ctatttggtt
    19321 ctttattcgg ctgctactta ttgtcactac gttatttcgc tagagattat cggttgctgc
    19381 tgctgaactg gaccaatatt aaccaattat ttaacagagg ttcaggaagt ttgggtttag
    19441 tcgcaactat gatgggttca ttgatcatca gtattattct tatcgtctta tacggaatgt
    19501 tggctttgtt ttatccattt tggttagtga atattcctgt gatcggttta gttctaatac
    19561 tttccgctct atggatcgtt tattatcaac gaacgttttg gaaaaaattt gaataaagaa
    19621 gaggggccgt gacaaaaatc atgtcgcagc ccctttttta cagatagagg gtgttcaaaa
    19681 gtcagtttct cagtattaag tcaattttat gtaaaaatgt gagaagctat ttttacaaaa
    19741 ctttttttaa tactcataat tacttcttat tgctcctgcg gttacttgtc gcaatgtttg
    19801 tgagatattt tgtcatcgct caaggctgaa cgtctctttc acaacctttt ctttgttaca
    19861 cttcgtttat ttaaaaagcc gtttttttcg tttcatcatg attgcgtcac gctttacttt
    19921 gaaattacgg atgttatggt taaagacttc tgataataca agacctaaag cgattgctcc
    19981 agcaatcata attgcttgga ccgcataatg agccgcttct cgataggatt cagtaacgag
    20041 atttcgaacc gcttgataag ctaaaccacc agggactaga ggaaccatcc cgggaatatt
    20101 gaaaatcgtt acgggcattt tcaaacgttt agagaaaaga tcgctgatga atgctactcc
    20161 taaagaacca agcaatgaac caaaagcagc agatccgcct atttgaacac agatccagta
    20221 gatcatccag ccaaaagcac cagaaagacc acaacaaatc aatgaacgcc taggtacatt
    20281 tgtaataacg gcaaatgccg cagtcgctaa aaaactaaaa gaaaactgaa taaatatcgt
    20341 ccccatactt gtgttccttt cctaataaaa aagctggaaa ataaaagcaa tcgcaaaacc
    20401 gatcatcgta gcagtcatca tcgcttctgt tccgcgagaa actcctgaga cataatgtcc
    20461 agctagcaaa tcacggatag cgttcgtaat ttgaactcca ggaactaaag gcatcacaca
    20521 tccgataatg attagatcct gattgcttcc taagccaaaa cgtacgctgt agatagcggc
    20581 tatgccaatc aataaagaag ataaaaattc cgataaaaat ttgattctaa aaaatttcaa
    20641 actatataga taagctgcgt atcccaatcc tccaatgact aaagtcacag gtaaatcggt
    20701 catctttccg ccaaataaca ccataattgt tccactgatc actgccgcgc taacgatttg
    20761 caaccagaca ggaaagaaag aacggtctcg gtcgattgct tgcagttcgc agtaaagttc
    20821 ttctaatgtt agctctgaag caacatattt acgtgaaaga ttatttactt ttactacttt
    20881 ttctaaatta atcgaacggt tggtaatttg ttccattcgg atggtcgagg tacgatcaag
    20941 accaataaag agaccggttt gagtgacata gctgaccaaa cggaaatttc cagaagctaa
    21001 agcaattcga ctcatcgtat cttctacccg atacatctcc gcattgcttt ccatcatgat
    21061 tttcccagcg agtaaacaag tgtccagcaa cagatctaat tcttttcccg tcatagcatc
    21121 ttctccaata attgattgat tagttttatt ttactacaaa acataccaga agcgtaaaga
    21181 ataaaaaaac ttgaggaagg aataaaagag gcgtatactg tttttgtatt gcgggtgtag
    21241 tttagtggta aaatcccagc ttcccaagct gatgtcgcgg gttcgattcc cgtcacccgc
    21301 ttaaaatagg ttgtgaaaaa gctgctctgc aacaagtaat aagaacagcc aagcaaaaac
    21361 aacttcttat gtttttaact tggatggact tattaccgca gatgcaagct tttgcgcacc
    21421 gtttaccaag cggttgtgaa atcagtgtat tgtcttgagt atgaagaaaa tttttgaata
    21481 ataataaata ccttacagat agagtggtga ctgattagag acgaggaatc cgtctctttt
    21541 ttgtattttt aagccatctt aaaacaggtt ttattttttg ctgtttctat ttctttctag
    21601 tatttattta taggggaaca tatgttaaga tttagggagc tgttagtaaa ggagtaaaga
    21661 aatgagcaaa aaaacaacca aggcgatcgt ggggatcgtc gctgctgtga ttgcaggggc
    21721 attcggtgtt tccaccacac aaaaagattc agatatcttt cagcagatta caactatttt
    21781 tgattccaca ccacaagctt ctcaagtagc ggatgtgcca gaatatgatg gaaaacatca
    21841 agaaatagaa atcaatggga atcagccgga atttacaaaa gaggatctta gcctttccaa
    21901 aggaacatgg gaaagctatt caaatattga taaattgaac agagtaggac aagccaatgc
    21961 tatgcttgga aaagatttgt tccctactga aaagagagag cctttgtata ttgacccgac
    22021 aggttggaaa caaaagaaat taagtgacgg gcaatggtta tataatcgca gccatttaat
    22081 tggtttccag ctgactggtc aaaataacaa cattaaaaac ttgatgacag ggacacgttc
    22141 gttgaatgct ccatacatgc ttgctcacga aaatgatatt gcggcatata tcaaagaaac
    22201 aaatcatcat gtacgctacc gtgtcactcc tcattttgag gggaatgaac tggtagccag
    22261 gggagtgcaa ttggaagcag aaagcattga agacaaaaaa attgaattca atgtctttat
    22321 ctacaatgtt caagatggct acacgatcaa ttatgagaca ggacaagcaa caaaaacaaa
    22381 aaattagtag cagctattcg aattttttat gaatattatg aaaaaattgt gtaaaaacat
    22441 acattcaaag aaatatagcg tatactttat gaaaagagta cttttgataa agctacagaa
    22501 aaaaggaaaa aaacatacaa atagtgtttc ttaatttatt cggaagtttg gaatatttgt
    22561 ttgtagtttt ccctttcttc gagtaaaata aggttgtaga aggtaagctg gaattacctt
    22621 gcgggaaaga ggtgtagaat catgggtttt acagatgaaa ccgtacgttt tggttttgac
    22681 gatagtcgca aaaaagaagt gagcgaaaca ttagcgattg tttatcgggc tttggaagaa
    22741 aaaggctaca atccaatcaa tcagattgtc ggctacttgc tttctggaga cccgtcctac
    22801 attcctcgtt atcgtgatgc tcgaaatttg attcgccgtc acgaacgcga tgagatcatg
    22861 gaagtaattg tcaaggacta cctctcaaat catggagtga atttatgaga gtaatgggct
    22921 tggatgtcgg ttctaagaca gtaggtgttg ctatcagtga cccctttggc tggacagctc
    22981 aaggagtaga gatcattcgg atcaatgaag cagaagaaga gtttggcttt gatcggttga
    23041 aggaattagt agcacaatat gaagtagacc ggtttgtagt tggattacct aaaaatatga
    23101 acaattccat cggaccaagg gcagaagctt ccatgtcata cggagaaaag atcaaagaac
    23161 tattctcctt gccagttgat tatcaagatg aacgattaac cactgttcag gcagaacgta
    23221 tgctagtaga gcaggcaaat acttcaagag ccaaacgaaa aaaagtcatc gataaattag
    23281 cagctgtgat gatcttacaa aattatttag acggctcagg taagcaatta ttttaataat
    23341 aaaagaaaag aggtaatctt atgacagaac ataaccattc ccacgaccat gaaggacacg
    23401 aacacatcac attggtggat gaccaaggaa atgaaacatt atatgaaatc ctactaacag
    23461 ttgacggaca agaagaattt ggacgtaact atgttctttt atatcctgct ggtgtttcag
    23521 aagatgaaga tgtagaatta caagcttatg catacatcga aaacgaagat ggaacagaag
    23581 gcgagttaga acaaatcgaa acagacgcag aatgggacat gattgaagaa gtattcaata
    23641 cattcatggc ggaagaagaa gaatagacta taaagctagg agaaaccttg atatgatagg
    23701 gtttctcctt tttttatttt ttactgtatg ccatattgta tgccatctag tcaatattta
    23761 agtgttgtac taatttttca acagtctttt ctttcgtttc ttttgccaag tgaccgtaac
    23821 aatcgatcgt ctgactaatt tttgtatgcc gtaatctagt ttgaacttct accatagtag
    23881 ctccagcgtc caagagcatt gaagcatgag tgtgtcttgc tacatgcaca ccgagaaatg
    23941 gaaccatagc tttttcacaa atagtaatca atcttgcata aatagcgtgg tgtgtgatta
    24001 gttctttata gataccacaa aaaatcatag tcggatcagt tacaccaagt gtcagcatat
    24061 atttccgctg attaatctgc cattttttta atatagaaat tgttctttca tctagtggaa
    24121 gaaccccagc actacaatca gttttaggat cactgatttt atagccattt ttggattggc
    24181 ttagtgtttt attgatggtg accgtatttt cttgaaaatc aatgtctgac caattcaatg
    24241 caagggcttc actgatccta accccgctat aaaagagaaa gcgtagtaac gttttatcaa
    24301 agtcgtttcg ataagtttca ggtttgctgt ttaggtaatc aaacaaggtg atcagttgtt
    24361 ctttactgta aactttgacc ttttctttat gcttagtggt ggttctttta ggcattaata
    24421 cgttgtctag tggattagtc tcaataatcc caagattgat agcgtagcta aaaatacgct
    24481 tagaagttga atgaagtacc ttgtattggt cagtagtttc agcccagcta tttacaattt
    24541 gttgggcttt ttttaccgtg acttcattca cacgaagatt tccatatttt ggaagaatcc
    24601 actttctaaa cttgatttca gttttcatga aggtggcttc tcggacagtg gttttatatg
    24661 tttctagcca caagtcataa acttctttaa acgtttcgtt gttcgctttt ttaataccgt
    24721 ttttatcgta gtccacttgc aagcgtgaca aggcaagctg ggcttctttt tttgttttgt
    24781 aacctcgtct acgaacctta tcaggtttgc cagtcatttt gttcattcca agataggctt
    24841 catagagcca aaatttacct tttttagttt catattgctt gaatgttgcc ataatagttt
    24901 tccctccata gtgtagcaag ccgtgggggc ggttggagga ggcagtttat tattattttg
    24961 aatagtgttt atttagctca gtaacaagag ctgtgaactc ttttagaaga tcattttcat
    25021 aaaattcttg atactctttt ttctcatctg tatttttttc tgaatcatta attaatgagt
    25081 catatcgtaa aagcgtattt agaagagctg cgagataagc aattggggta ctcccgtcag
    25141 gaaattcaat tcgagatcct gttttaagat attttattgc gtttgttaaa taaatcttct
    25201 gtgagacagt caggttatct tcttcgatgt tattgatagc attagaaaga ttagaatcaa
    25261 ttatagagtt atttttttca atagtttttt tcaattgctt ttcaagcaca tttcgcttaa
    25321 tttcgggcat catatgcata tctcttaatg tataagatcc gtttaataga taggtagtgg
    25381 atacatctaa tacctttgaa agttgtttaa gcctggtgtt attgggaaga ttatagccat
    25441 tttcccaatt actaacggca cctttactgg ccggtgggga taatttacct ccaagttgtt
    25501 ccattgagag tcccttttca attctagcca gtctaattct ttgtcctgtc tccactgctt
    25561 cggggtattt ttttctagcc acattttcac caccattcat atattttctc tactatatca
    25621 cgatttaaat atgtgtgaaa cataaaagtt caaaaaagta tgttaaaatt cttgcaaatg
    25681 aaaaggtatg atggtatatt gagtttataa gaggtataga aatgtattca agagcctctt
    25741 gcattttttt aaaatctttg tatagaaatg tatgctaacg aggggggata aatatggaat
    25801 taaaaattga caaactcacg cttgatgatc cagcttttaa aattattgac gatcgagttg
    25861 ttcaacaagt agagcaaact caaggaacgt tactttcatt attagaaaaa aagtaccagt
    25921 ttccaaaata tatgaacaag gcacaagctg ctaagtatat gaacgtttca tacaatacta
    25981 tgatgcagaa atatgttcct aacggtttaa agttgattat agttgatgga gtcgtaagaa
    26041 tttcacaaga tgaatgtgat cgctttatgg aagaatacca aaaataaacg caagccgtgg
    26101 gggcaaaggg attaatagga ggaaataata atggaatcga cacaaaataa agcaatcgaa
    26161 aaagtattat caacaattct tacagaagac acagcggcag aattagcaaa cttggaaggc
    26221 aaagcgctag aagaaacgtt tgaatgtttg tacgaacaaa tggactatca aaaattgcta
    26281 ccacaagaac caacggtaag cggagtattg cgtggcttga acgatttagt acaagcagag
    26341 tttaaagaac gactttctat tgaagagtat caagaaattt tgtatcagca agttgagttg
    26401 ttagcgagct tgctagggct tgaactggag gacgttgaat gaaaactgaa atttggaacg
    26461 gacacattat aagatttgtc gatattaatg atgaatggtg ggcagtggcg aaagatgttg
    26521 cggaagcgtt aggtcttaaa caggttacta gagccattca ctctttgcct aaagatgggg
    26581 ttactactag taaggtcatc gattcattgg gcagaacaca agatgtaaac atcatcaacg
    26641 aaaaaaaata tttaccgcat ggcattcaaa agccgtaaaa aagaagccga ggcatttcaa
    26701 gactggattt tcgacatcat caaagaacta cgccagtcca caggacttga aggctttcaa
    26761 gtattccgca tgctagacaa agaacaccaa aaagaagcaa tgactaaatt aagccacgct
    26821 attactgaac caaaaccagt agactacatt aaagcgaatg tgatagccaa taaagctgta
    26881 tcaactattt atggtcattc caaaatggta aaaaagaaag atatgactcc tgaaatgttg
    26941 gttgatcgtg aacctatttt agatgagaca gtagaattaa tgactgttaa agaaaaatat
    27001 gggttgcagt tttcagtgag tgagaaaatt tataatcgtt ctgctgaatt gcagacaact
    27061 taggagcatt aacatgatcg agaacaaaga aaaagcctta tccataacag tcttggcgga
    27121 caggataaag gcaacttaat tgggataaaa cccgatattc tttgttccga ttataccata
    27181 ttaaaaaatc ggagggaacc attatgaaac gaaacttaat tataggaagc attttaatcg
    27241 gcggaatttt cggcttatta ccattaattg caaaaatagc tatctttgca ggattagcgt
    27301 tatatctctt cactgcttat gacgagtacg actatcaatt aagaactgga gggcataagt
    27361 agatgtacaa aacaaaacta ttaaaccaac tcgatagcct tgaattagag gaaattaatc
    27421 aagggatcgc tgaactagaa aataatatcg ggaaaaccta ctttggaaat tcattcaatg
    27481 aaaaactaac agttttgtat gtactcaaaa aacatgcaga acacaaaata atttgtcgag
    27541 aaatcaacga gctaaaaaat cagattttaa ccgcatggtt aaacattact gatatgcaag
    27601 aagcaagggt gaaaacattt aatacatggg taaagtatca gaatcaatta aaaggtgctg
    27661 aatttgttcg ggatgggttg aaatacgaac ttgagcagtt gaagctcatg gaggtgtctg
    27721 aatgatttat accagtattt tgaaaaagga ggagaggttt attggcaaat aattatcaag
    27781 cagcaacgga attacaaaag tcgggatggt caatttatcc cttatctcct ggaactaaaa
    27841 ttccaataaa aggctctagt ggttggaatg atgcaactaa cgattctaat caaattaatg
    27901 aatggtggac ggaagatgca actagaaata ttgggttaat gcttgaagat catcaaatgg
    27961 tggtagttga cctagatcaa cattctgaaa cacgaaacgg cgttgagaat tataaaaaac
    28021 ttgcttcaat gtatgatcca tttccttcta cttatacgga aatcacacca ggtaaaggaa
    28081 tccatttctt ctttaaaatt cctgatggtg taattatcca acaagaaaca ggtgcatttg
    28141 ctgatatctt cgggcgtgat gataaaggta aaagtttaag cgggattgat attatcacta
    28201 aaggagttcc aatagcgcca actatgatta tttcaagaag tgttgggaga gcatatgaac
    28261 ctattgaaaa cgtatctcta aataatattg caccagctcc cgattggcta gtgaacctac
    28321 tgctaaaaaa accgcaagaa ataaagactc aataccgacc cgtagcaaaa acagggacag
    28381 caaaaaaatt agatatgatt gttcaaggcg ctggcgaagg tagtagaaac gatcatttaa
    28441 ctaagttatg cggttggtta ctatggcacg gtgtagataa tcaaacttta gcagaattga
    28501 tttatacagc aaatgcgtac aatgaaccac cgctaaaaga taaagaagtg aatcaaatta
    28561 ttcgatcgat gattaaaaaa gatttgaggg ggaaagctca tggctcataa gcataaatta
    28621 gatagtattt tagactttcc cgaagccagc gagcgtgaag acaatatcat tgaattgaaa
    28681 acctggatgt cacgattaag atgcaataaa gacgatcaga ttaaaagtaa cagtgttgtg
    28741 aatgcagaat tgattttaac caatgatagc aatttagctg gaacaattgc ttataacgaa
    28801 tttagcggat acattcacct attaaaagat tctccttgga tcaatcgtac cgctggagaa
    28861 tgggaagaca gttttgagga tgccttaacg gcatatattg aagaaaacta caatgtagtt
    28921 tttgatgaca acaaaataca caaagcagta gtaaatgtgg ctagaaagaa tgttttcaac
    28981 ccagtaaaag aacgtatcga aaaagttaaa tgggatcaac agcctagact ggaaacactg
    29041 tttattgacc ttttaggggt agacgacaac ctttacacac gagaagtaac aaaacgttgg
    29101 atagtcggat cagtcgcacg tatctataaa ccaggaataa agtttgagat cgttccagtt
    29161 ttggatggac cgcaagggat tggtaagagt accgttccag cattacttta cacggatgat
    29221 tttttcaccg attcattaga ttcgctaggt gaaaagaaag atgattatat gcagcttcaa
    29281 ggcaatgtaa ttattgaact tggagaatta tcttcaatga acaaaactaa aattgaacag
    29341 ataaaaaact tcatcagtgc caagatcgat aaaattagac ctccttatgg tcggaatgtc
    29401 atagcttggg caaggcaatg tgtatttatt ggaacgtcga atgatgggca gtatttgaaa
    29461 gatgatacag gaaaccgccg tttttatcct ttgccttgta agaataagcc aaagatgaat
    29521 ccgtttaaaa caaaggatga atattttctt caagtgttag ctgaagcaaa ggtgcttttt
    29581 gataaaggac aacgaatcta ttttagtccc gatgaagata aagaagtctt agaaattgca
    29641 aaagactatc aagaggatgc caagatggag aatccaatta aagaagcaat cactaaatat
    29701 ttggaaatgg aaatcccgta taaatgggaa gaagctccag cgtgggcgag acgttcttat
    29761 tatcagaact atcctgacag taaatgtaat gagaaaaatt tacagcattt ctataatgga
    29821 gctactatgc aacaagagtt ttatttagtt gatagcgtac tcaccgctga tattttggaa
    29881 gctgtttttg ataaacaagc aaaggattta ttgaatggtc gatcagatgc agaaactaaa
    29941 aagattgcct tgattattag cagtattcct ggttgggaac ggaagcaact gccaaaaaga
    30001 aataaacgac gaggatttta caataagtca aatgcctatg aaaataaaaa tcgagctgga
    30061 aagtacaaaa agtaacgggt agtaacgggt cagtaacacc tagcccgtta ctagtttacc
    30121 cttactgccc caagggatac agcaatttgg taacggggta acaccttatt tccttaaaac
    30181 ttttgaaata gttagctaat atgctgggga ataatgttac tggattttac ccgttacctg
    30241 ttaccaaaac gctagaaccc ttgtccccgt tgggattgag tggtaacggg tacctgttac
    30301 gtaggtgtta ccacgtgtta cgaatcaagg aggaaagtaa atgctcaata ttatttcaac
    30361 aaacaaagca ccgaactttc aatatacaga tgaaatggat cggtttctaa tgaacacgct
    30421 ggcatttagt gtgggattgg taacagaaga ttattccaca tttgatccag aagtattaaa
    30481 gataatggaa gaagaacctg attggctgca ggagtcagta gcgtggtgtc aatcgctagt
    30541 agtgggatca ttggtagata gtggcaacta tgatgataca ggtgagctaa tggatgaatt
    30601 taattgtctg cttaacctat atgatcgagc gagacaacga gagcttacat cgaatgagga
    30661 taatttgttt ttaaacatcc atgataagtt cttggctttg ctactaacag atgatgaatt
    30721 gataactaat ttattggagg tagaataatt atgcatatgg aaagtattag atacttcgat
    30781 ggtgagaaac acattgagta ttcaaacact caagaagatg tagactttat tagttttgag
    30841 gaagataaaa tcactaaagt taattttaaa gacggaacgt gcttgaagat agtttcgcct
    30901 tatattgaat acaaatcaaa atgggaacaa gatgattctg acgaacgaga tcgctttggt
    30961 tggtaatcta tggcagttaa aaaacaatgt aatcatgctg gatgtaaaac attgattgat
    31021 tataggcaga agtattgtgg gaagcataaa gcaaagcaaa cggcaattaa gcgtgaagaa
    31081 agaaagcaaa gcgaaggtaa atattttcaa ttctatcaaa gtagaacatg gcgaaaggct
    31141 tcatacttgt atcggttaaa tcatccagta tgtgaggatt gcctagaaga aggcttgata
    31201 aggaaagctg atgttgtaga tcacaaaatt gaactaaaag atgattggaa tagacggtta
    31261 gatgaaacta attttcgttc tttatgccat gcacaccata attccaagac agccaatgaa
    31321 agacaaagac gagaaaagag taccctttga gttaaggggg catagtgtga aacttactga
    31381 caatcgatgc ctactcattt tggtacaaat aaccgttgtg aaaaggcata agggtaatta
    31441 caaataacga ttacacttgt aatcacaagt gaaaaagtat ataatgaaag taggagattt
    31501 attgaaaaat acaattgtta ttactgataa aacagggaaa cagcgcacca ttgagttacc
    31561 tcattttggt caagtgacca ttcaaatgca gaacggaaaa atcatttata tagataaatt
    31621 agataaagaa aaattctgat cgaaaaacga aggaatgtga gcttaattgc ttacgttcct
    31681 ttttcttttg tctgaaagga ggacaacatg ggacacccta aaatattaca cgatacaaaa
    31741 ggcaatatat caagtgaaga aaaagcaatc cgtgtggacg ctagagaaga attgttcaag
    31801 cagcaaccat taataaatat cacgccccct gactggatgg cagcgagcgc tagaagtgaa
    31861 tggaatcgta tcgtaccaac attaaaaaaa gattatccat tgagtgaagc agattatggt
    31921 tcattggtag cctattgttt agcctttgct cgaatgaaaa cagccgaagc cgagataaga
    31981 aaatcaggta cgtttatcac atgcgaaaac ggagtaaaga aagccaatcc agcggttcgg
    32041 gttcaatcac aagctatgag tgatttgaaa aaacaagcca cctcactagg tatgacctta
    32101 gaatcacgat caaaactagc tttgaacaag gttaaaaatg atgaacccga agacccgttc
    32161 aaagagttga tgaattcatg aatgattaca ttgagaaagt cctatcaggt aagctgattg
    32221 cacccaagaa aattatccaa gcgtgtgagc gtcatataag cgatttggag cgttctaagt
    32281 cagatagctt cccttatgtg ttcgatgaag aacaagccac caaagcgatt aaattcattg
    32341 agttgctacc atctacggac ggtaaagcaa tcaagatgtt aggatttcaa aaattcattc
    32401 taggcagtct ttatggctgg cgtactaaag aagggaatta caggcgattc aatcgagcgt
    32461 ttaccagtat gagccgtaag aatgggaaaa cgtatatcgc aagtggcatg gctgccaatg
    32521 cgttgattat ggaacaagaa ccagcagaag caaggcaagt attgtttgta agtaacgctt
    32581 tgaaacaagc taaattgggc tatgatatgc tgtctaattc acttagaaac gtggtcaagt
    32641 ctagtaagtt tttaagacca caactgaaaa ttatgaactc taagattcag cacttgccct
    32701 ctaattcgtt cgctatggca ctggctagtg aaaccagcac gctagatggg tttgcaccaa
    32761 caaccgcaat tcttgacgag tggcacgaag caaaaactcg taaaacgtac aacgtcatta
    32821 agtcaggaat gacccaacaa aagaacggct tattgtgtgt tattagtacc gctgggcttg
    32881 atttaaacgt tcctatgtac gaggaatact tattgttaga tcgtgtgcta aaaggcgaag
    32941 aacaagccga caggtacttt atagcgatat gggaattgga cgatcccgaa gaaattcacg
    33001 atcaagagaa atggatcaaa gccaatccga tttttgaaag tgaagaaatc aaaaaagtaa
    33061 tgattccaac cattcaagat gatgtgaacc ttgctttgaa acaaaataac cttaattctg
    33121 tattggtgaa aaacttcaat ttatggagac aagcgagtga ggacagctat atgattgctg
    33181 aagactggca ggcaaccgaa gtagaaccac aagatattac aggcaagccc gtttatatcg
    33241 gagtggattt atctaaaaca gatgatttaa ctagcgtttc atggatcgta ccgctagata
    33301 acggcgaact ttattgtgat tcacatagct ttgtagccac caaatatggg cttcaagaca
    33361 aagaaaagcg cgacggtttg ccttatcgag aactagaaaa agctggtgag tgttccatta
    33421 ctcaattaga aagtggaatc gtggactatg accaagtatt tcagtttatt caagatttga
    33481 ttcaagaaaa tgatttggaa tgtatgggga tttgttatga cccgtataac gctaattcgc
    33541 ttatcagtaa agctgaaaaa gccaactacc caatgttaga agtaagacaa ggaacgatta
    33601 ctctaaacgt tccgacccga acttttagag aacaagttta tgaaggcaac gttattcaca
    33661 ataaaaatac gattctcacc catgcagtga acaacgctat tttaaaaacg gataacaacg
    33721 gcattcagat taataaatca aagaacagta acaaaattga tccaatagct gcattaatca
    33781 atgcctatgt gtttgcaatg gattacttca ccacaacgga aggagcgaaa gcagacaatg
    33841 aattttatac aagtgaagaa ttttctttct aattacattc ataccgttct tttacttctt
    33901 ggattggtgt gtgtgttggt tgctatcacc ttactaacaa atgtctacta tggcttgtta
    33961 gcgctgggca tagtgcttat tggggtagcg gtcatgctaa atacagaaca gaagggaggt
    34021 taaaagatgg cattttttaa agcgagacaa aatacaacgg gagatccttt cctacaacat
    34081 gtggtatcaa tccaatcgga tgattacacc accagcttta caagcgttcg tgcattaaga
    34141 aatagtgatg tgtttgcagc cgttcgaatt attgctagtg atattgcttc aagtccgatt
    34201 caattggtca aaaacaatat gccacaagct gataatgaac tggtgaagtt actaaacgag
    34261 aaacctaatt cagaaatgga tggttggcat ttaaagtttg ctttggcggt caatatgttg
    34321 ctaaacggta atagctttgc agagattaaa cgcaacggtg aaaaggtaga agaaattcac
    34381 ttattaccta actcaagtgt aacggttact caattagata atggtacgtt gtcttatcag
    34441 attggtgata aaaaaagacg tgtgaagtct agcgatattt tgcacttcaa atatttcacc
    34501 caagatggtt taacaggatt accaccactt tatgcgttac gtgatgaact gaaaattcaa
    34561 caagctggca accgcacatt gcacaatttc tttactcgtg gtgtcagtgg gtcaggtatt
    34621 ttgaaggttc ataagtcaga tttagacgga tcagcaaaga acgcaattcg tgaaaagttt
    34681 gaagaagcca acggttcaag tagtggagat aatgctctta gaacgatcat tcttgatgaa
    34741 acaatggact acaaaacatt agaaataaac accgatgttt tgaagcttat caattccaat
    34801 gattggaaca caaaacaaat tgccaaggcg tttggtgtac cgattgagcg tttaggcgtt
    34861 gaaaatgaac attctagcac cgttcaaagt aatctccagt atatccaaag tacgctgatt
    34921 cattacttta atgtgtttgt gagtgaatta gatacgaaac ttgaaaccaa tatccgcttt
    34981 aattccgatc agttactaga gactgatcct gaaacaaaag taaaaaatgt attggatcag
    35041 gtcaaagggt cacttctcac gattaatgag gggcggtcga aaatggggct accccccatg
    35101 gatggtggcg atcgtttact agcaagtttg aactttacgt atttagatac gttggagaaa
    35161 tatcaattaa aagaacagga aggagttaca ccagttgaat aatgaagaag aaaaggaaaa
    35221 acggctgaca gaagaagctg agctaacagc cgattctccc aaagtagaga aagaaaatga
    35281 aaaacaacca acagacggca aaactatttc aggctatgcg ttgaaattcg ggcaaccgtc
    35341 aaaagattta ggcggctttg tagaagtgat tacacccgaa gcattaaaag aggtggattt
    35401 atcaaatgtg ttcttattat atggacacga ttattcaaag ccactagcta gtgtcaaagc
    35461 aggtacgttg aaattgaatg tcgatgatac aggattacat tttgaagcag agctaaccga
    35521 cacgacctat tcaaatgatg tgtatgagaa tatctcaaaa ggggttgttg attctatgtc
    35581 ctttgggttc gtgcttggat tagattcatt cgacaaaaaa gaagatggca caattgaacg
    35641 atcaatagat aaaatcaaag cacttaatga aattagtgtc gtgaccgttc ccgcttatga
    35701 ttcatcaaat gtccaagtaa ataagcgttc ttacgaatcg tttatgagta acaaccaagc
    35761 aaaacaaaca aacaatagct tagaatccac ttctaaagca caaaaggaga gtaataacat
    35821 ggaaaaaact ttaatcgata acgaaaaaac agaattgcgt gggtatgaag aatatatccg
    35881 ttcacaaggc gaagtgcgtg atggagtcac tactgtaaat gcagcggcag ttgttcccga
    35941 agaagtaatc ggtgaagtct ttgatttgaa acgttcaaat tataacttag ctcaatatgc
    36001 aacagtaaaa acagtatcaa atggacaagg taaatatcca gtagcaacta accaacaagc
    36061 agtgttagca acaaaagctg aacttgctga aattggtgat attgacgctg aaatgtttac
    36121 ttcagttgat tataaagtag aaactcgtgc tggtaagatt gccttatcaa atgaggttgt
    36181 ggaagattca gcagtgaata ttgtacaaga ggtcaaagat cagttagcga aattggtaga
    36241 aaacactgac aataagcata tcatggattt attaaaaaca ttcactaaga aaacggctgc
    36301 tacgttggac gatttgaaac aactatacaa tgtggcatta gacccagcat taaataaaat
    36361 ggtaattcta aaccaaagcg gatattacca cctagataca ttgaaagatt cagatggacg
    36421 ttacatttta caacccgatg tgacagcacc tagtggtaaa tcattattcg gtatgccagt
    36481 agtattgatt gcagatacat tgtttgccaa tcctaaagct ggaacattcc ctatgattat
    36541 gggggatatt gcacaatcta tctttgttgc tcgtagaaat caagtaacga ctcaatggga
    36601 aaaattcgat tactactcac aaggacttgc agtgatcgtt cgcaacgatt ataagaaaat
    36661 tgatcaaaat gcttcagtgt atattgagtt tacgccagtt gtaacaccaa aagtatagaa
    36721 aaatattggg cggcggttta tcccactgcc tttttttcat taggagagat aaaatggtaa
    36781 ttttggatag cataaaaaaa agtatgcgga tcgatcacac tattgatgat gactttattc
    36841 aacaattgat tgatacagca ggcgaatata taaaaagtgc tattgatagt agcgcaacgg
    36901 ataaagatat ggataattat cagcaatttg atttggcggt gtcattactt actcaacatt
    36961 ggtatttgaa ccgtcaagaa gccagcagcg aacggatacc agtaacggta caagcattag
    37021 tacaacaaat gagaggtgct tattatgcca atcattaaga atgtaaatga attgactgag
    37081 agagtcaagt ttaaaaagac aaaacgggta aaagatgaag atgggcagat ggtggatagt
    37141 gaagaaactg tatttgaatg ttgggctaat gtgcgttcac aaatgctaaa agacgtgctt
    37201 gctagtgtag gtaccattct tgaaggaacg ttgacgttta ttattcgata cgatcaagat
    37261 tatgaactaa caaacgatat gaaagtagct tggaaaaaca agagttataa aattatttca
    37321 atcaatgaag gaacggcgtt taaagattat acaacgatca tagctaaaca gatttcttag
    37381 aatgattaca cttgtaaacg ttgtgggtat ttagtataat taaggtagta aatgaagggt
    37441 tagctgcctg aattttgcaa gacttagcta gtcgaaatct attgttggac tgaaaattgt
    37501 aggtgtggtt gcaaacatgc tggactaaga aagatgatag atgatgaacg ttcaatttta
    37561 atacaggcat gctaggactg attggatggt tttggcaagc tgattgtgta gaaatcaaag
    37621 aacacgtctg ttgtggacgt gttttttttg ttataattca aaagaggtga tttaatggac
    37681 aaagcagagt ttacaaattt ttattggaag tattatctac atttagaaaa tgagtttatt
    37741 aataccacag attttgtgat gctcgatgag aataatttca aaacattctc tattgaatat
    37801 caaaaactac tactagcgat tggttcggag tgtgagatta ttttcaaaga actttgcgga
    37861 tttgatagca gtagcaataa aagcataact gattttaaag ctgttattaa atctagcgaa
    37921 ttaatgtgtt tggataatgg tgttagggtt atgaattctt taacattgac ttcactaaaa
    37981 ccttttggag atgggtggcc tgaagataca ccagattggt ggaaaatata taacagagta
    38041 aaacatggtc gttcttcaaa ttataaggaa gcaaatttgg aaaatgtgtt gtatgcttta
    38101 gcaagtctat atttattaga ggaatatttg cataagaaag taatactaga aggtgaagta
    38161 gactttataa gtccagagtc aaatctattt acgttatctt gggaaagaaa acattcgtcg
    38221 atgcaaaaag tgacgtttga aaaagtggat tcaaattatg tagctccact cattgatttt
    38281 aaagaacaag aagaaattta aaaaccccca cggcttattg aagtttttgt atgccatatt
    38341 gtatgccatt tggtttaaat cgcttgaaaa atagtgatag ataactctaa ttcatttagt
    38401 cataaacgct gttttgataa cctttatagt tctttgatat gggtttctca ttcatggcgg
    38461 aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg attgtgacaa
    38521 acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc ccctcggcat
    38581 tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc taataatcag
    38641 gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca actcctcggt
    38701 cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact
    38761 cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
    38821 cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg ctgttcttat
    38881 tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa tttccttaaa
    38941 aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa agaaaagagt
    39001 aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg tctgatttat
    39061 tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg tggaggcgtg
    39121 ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat ttctttttac
    39181 tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt atcaagcggg
    39241 aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt tggaggagta
    39301 gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa gcatgtgcag
    39361 ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta caccaagcat
    39421 catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg gggggttctg
    39481 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
    39541 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
    39601 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
    39661 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
    39721 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
    39781 cttttgatca atctttacaa tgctttcgtc atcttacaaa cattaggaga atctagacta
    39841 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaggagta tactgtgata
    39901 aaaaaattca tttaaaaaaa taaaaattgg ctggtgattt ggatgttgcg ggaaatgcgt
    39961 ttaggagatg cagaagcact acgagaaatc aatgcggaac aattaggata tgatgtacca
    40021 ttaaccgtta cggtgagaca aatgaagaaa ctgttgaagg agccagaaaa aaattttttc
    40081 ttggtctatg aagatcctac ttctaaaaaa gcagctggct atgtacaagc ggagtcctat
    40141 gaatcgattt tcagcgatcc gatgttcaat attatggcac ttgctgtgtc taaacatgct
    40201 gaaaaaagag gaatcggaaa ggccttgatg actggcgtag aagaagcgaa aagacgagga
    40261 atatcagcaa taagacttaa ttctgcagaa tacagagtag aagcacaccg attttatgaa
    40321 cgcatcggtt atcattccga taaaatgcaa aaaagatttt tgaaaatcct gtaaattttt
    40381 atcaaaaaat acctggtcat caagccgata cttgatgatc aggtattttt tttgactaag
    40441 aaagtaattg catcaagaaa cctgagatga tcaaaacgat ggcaccacca agccgattgc
    40501 ccatttgagc aaaagcaata agttccatcc gattagctgc agataagaca gctacatttc
    40561 ctgtaccacc catcgagttg ttgcataagc cagcagtaat agttgattcg attggatata
    40621 aaccaaacaa acgaccgaca aatcctgaaa ctactgaaat cgcgataaca cttgtcaaac
    40681 ataagacaac aaattgccat gtcaaagcag aagccaatac atttagattc aataacgcaa
    40741 taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagttg ttgaacatga
    40801 tagctgcatc ttcgtaatat tctggtaata ggtttgcgat tttacagata acaacgataa
    40861 tgatcatgaa tgcgtaagca tggactttag gaacaaaata gttacagatt tgtcctaaga
    40921 tgaagaaagt caatgatacc atcaaaccga cacctaattg tggaatggaa ggttttacat
    40981 ttctaggttt tccatcgcca aggtcgcctt tttccattaa tttaccatta ccgttgaatt
    41041 ttggcattga ttgtcccgca cgagcaacta acgctgcacc aatgatagct aggacatttg
    41101 tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcgtccgta cctaaaacat
    41161 ttgcataaat cgttgataaa ggtacagctc cggctccaac accaccagcc attgcgggca
    41221 atgagatata taaaacggaa tttgcaaaac catttccgat aagcatacct acgagtccta
    41281 cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga acagaagctt
    41341 tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca gcaatataaa
    41401 agtctaaaaa gcccatattg ctgacaaagt tttctgtgga ttttactaca tactcaggta
    41461 agatcccaaa tgtagcaatc gcggcagagg caaagatcgc aaaaacggag ccaccaccta
    41521 aataactttt gacgataggg atttttgttc cgataaaatg aaataaatta ccgaaaatga
    41581 ccaaaacagc gattgggcca atcatattcg tcggtaattt tcctaaagcc attgcaatga
    41641 tcaggacaag gaccatgatc aaataaaggg gcaaacttac gccggtaatt tttgttgacc
    41701 aaaacccttc ttttttcttt tcctcaactg cagttgcggt tgttgttttt tgtgccattt
    41761 ttacagatcc tttcttattc tgtatcgtat gaaaaaagcc aaagagaagc ggcaaaagag
    41821 cagtagttgg ctggctgctg ttttgccgca gtcgcttttt tctttttaga cgtcatacaa
    41881 agtttctgta tgttctttta ctataacaat cttaatcttc taaagattta tattcaggaa
    41941 cccatttagc agattcaaca gcttctttga tatccgtgat ttcttcacga ttcaattttt
    42001 gtgcaacaac tgattttgcg acagtttcag caatcgtttg agagaattca gtgatttttg
    42061 ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg cctaatgctc
    42121 gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca atcaaaccaa
    42181 gccctaaacc tgggtacatc aacgcattat ttgcttgacc gatttggtaa gttacaccgt
    42241 tatattccac atcagcagca gggatacctg ttccaactaa agcttttccg tccgtccatt
    42301 caatcaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa ggcatgatga
    42361 ttggacgtgg ggtatgagcc gccatttctt tcacgatttc ttcagtgaat gctccaggtt
    42421 gggtagaagt accgatcatg atcgttggat ggattgcttt tacaactgct tctaagtttg
    42481 tcaattcatc agcattagtg aattctgagc gtttacgagc gaatgggatt tgaccaggag
    42541 tcaatccttt tgtatcttca aacaataaac cctgtttatc tacttgatag aaatgttggc
    42601 gtgcttcttc ttcaggtaca ccttgacgga tcatttcatc taaaaggatg tttgcgatac
    42661 ctacaccagc agtaccagca ccaaatgtca agatcgtttg atctttcaat tgctcacctg
    42721 atatattcaa tccacctaat acaccagcta gtacaacgat tcctgtgcct tggatatcat
    42781 cattgaaagt agtgatttta tcttcgtatt tttctaagat cgttgcagca tttcctcgtc
    42841 caaaatcttc ccaatgtaat aacaattctg ggaataattc agtagctgat tgaacgaatt
    42901 gatcaatgaa ttcgtagtaa gtttcgcctt ctacacgagc atgacgattc cctaagtata
    42961 atggattatt caataattct tcgttattag taccagcgtc gattgataca ggcaagactt
    43021 gtgcaggatt gataccagcc gcagcagtgt agaccatcaa tttaccgata gcgatgtcaa
    43081 caccgttcac accccagtca cccataccta agattccttc tgcatctgtc acaacgatca
    43141 aacgaatgtc gcgtccatct gctgcgtttt tcaaacttgc tttgatattt tctggttcgt
    43201 cgattgaaag aaatgcagca tcttgtggtt tcaagaaaat ttcgttgtat tgttcgattg
    43261 aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt tcgcccatca
    43321 atttataaaa caatgtccgg ttcgtattga atagattcat taagaagata cgtttttcca
    43381 aatcggtctg tttgcttaag tattgaccgt aagcttggac agattgttgt tccaatgttt
    43441 ggacagtact tggaagcata ccagtgattc cgtattttgc ccgttcttct tttgtaaaag
    43501 ctgttccttt atttagaaaa ggattattta ataagtttag ccctgtaagc atgaatgatt
    43561 cctcctaagt tttaatacaa acgaagtata ggagtcagat gaggatatag tcaaaaagag
    43621 caggtataat aaattttatt tatatgagta atagagtcaa aagaaatcaa aatagcggta
    43681 tttgttcttt ttatatcaac aatccttgct tgattactat gcgtatctct tatatgctaa
    43741 agaaagaaaa gtagaagaat aaaggatatg ttagatagat aaatagaatc tctgaagaat
    43801 aaatcacgtg atcgtgacga taaaatacac ttacgtcagg tgataaaaaa tacataagta
    43861 gaaaactaag aggaggaaca gaaaatgaaa gaagttcaat tgatcagaaa aagcgaacta
    43921 tcagaaggtg gttgcaatgc atgcggcgta gtagaagcaa caagctatac attaaagctt
    43981 ggtgccaaca aagcgataat ctctgagttg acagtaggcg gtcttgtgga cagtctggcg
    44041 cttgctgaag gttttattgg agaagatatt tatgagatgt tttcagaaat ccgtcagtta
    44101 aaaaaagggg aaaactgcat agaggttcat catgagagcc cgaatgtgag attcaaacgt
    44161 ggtgataatg agatgatctt caataatcat gtgtccgatc acaccgagct atatggaatc
    44221 gtgaatcaaa tactaactga gctttttgga ttgggacctt atgcgttcaa ggaagaaaac
    44281 ggtaatccaa aattaaacga agaatggcaa gaaacgattg aaatacaaag aaacaacccg
    44341 cacttatttc aataggcatc cacagtattt cgtaaaaggg actttgacac gacttttgtc
    44401 aaagtcccat cgttttttat ctatttgtgt tctaaacatt tctttcagag tagtaacaac
    44461 tggaatgact gaaatgtaaa cagattattt gcctgtatca atacggaaga gtaatataaa
    44521 gtgtgtaaac gctatcatat tattcattca attttttata gagggaaggc attgaaatgg
    44581 aaaaaacgta tcaagcaggt acaaatgaag gaatagtgga ttttatcaat atggaagatt
    44641 tagaaatagc cgcttcacaa gtgattccag ctggtggtta tggttacatc agcagtggtg
    44701 ctggcgactt atttacttat caagaaaatg aacgggcatt caatcatcag ctgattattc
    44761 ctcatgtatt aaaagatgta gaattacccg atacaactac acattttgac aaagaaacgc
    44821 tgactgcacc aatcatcatg gcaccagttg ctgctcatgg cttagctcat gtgaaggcgg
    44881 agaaagcttc tgcaaaaggt gtagcagatt ttgggacgat ctatacagca agttcttacg
    44941 cttcttgtac attagaagag ataagagaag caggcgggga gaaagcaccg caatggtttc
    45001 aattttatat gagcaaagat gatgggatca atttggatat tcttgaagtg gccaagcgta
    45061 atggagcaaa agcaatcgta ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag
    45121 accgcagaaa tgggtttacc ttcccactgc caatgccgat cgttcaggct tatcaatcag
    45181 gagtaggcca aacgatggat gctgtgtaca aatcttccaa acagaagctt agtccaaaag
    45241 atgtagaatt tattgctgct cattctgatt tgccagtcta tgtgaaaggt gtccagtcag
    45301 aagaagatgt ctatcgttca ttagaatctg gtgctggcgg aatctgggta tctaatcatg
    45361 gtggccgtca actagatggt ggtccagcag cgtttgattc gttgcaatat gtggcagaag
    45421 cagtagataa acgtgtacca atcgtgtttg acagcggtgt acgacgcgga cagcatgttt
    45481 tcaaagcaat cgcatcaggt gcagatcttg ttgctattgg cagacccgtt atttatggat
    45541 tgtctttagg aggaagtaca ggagtccatc aagtgtttga tttctttaaa acagaattgg
    45601 aaatggtcat gcaattagct ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc
    45661 gtgagaatcg ttttatttaa gaaacaaatg acgaaaaaaa taggagtgcg gcagatgatg
    45721 ccgtactcct atttttatag ttcaaggatg tcactaatag aataatttga aacgaaaaaa
    45781 taaaattgaa atattttttc atacttaata aaaaaggaag aatattcatg taaacgattt
    45841 tattgttttt ttctctttta tacaaaaagg gtcataattt caagtaagcg gatgaattca
    45901 tgaggatttt ttgcatactg tctctactat acttaggtca ctaagaaaaa ggagtgattt
    45961 attatgaaaa agaacatgat tgggacatta ttactttcaa cacttcttct aggcggcttg
    46021 gcaacaccag cttttgctga gggtcaagcg acttcaaaag gagatattac ctttactgaa
    46081 ccaacaaata ccgtggagcc tcttaaccct actgatcctt caaaaccagt agagccagca
    46141 gatccggaaa atccagcaac tggccagaca ggtcctttaa cactggatgt agttcccgaa
    46201 ttgccatttg gtacacacga gattgaaagc ggaacaaaaa cgtatcaagt agacgcttca
    46261 aaaaacgata caccttatct ccaagtatca gatcgtcgag gtgtaggtgc agatggccaa
    46321 gcacaagggt ggaacgtgac agtttcggtt tcagattttg taaacggttc acaagttttg
    46381 caaggcgctg aattagattt tggaacatcc actgttaaaa gtacgtcaga taacgaatcc
    46441 actggaccaa ctagtcaaac tgtaactggg ttgagtaaag caagtgcagc gacaccaata
    46501 tttacagcag caaaagatca aggtctggga acatggcttt ctgtttatga tccagcaaat
    46561 atcacactga aagtgccgaa agcagcagct ggaacattca cggctgattt gacttggaac
    46621 ctcgtggctg gcccagtagc atagagtatg aaataaattc aacaaactgc ggtgaaaata
    46681 ctataggtga aggatttttt accgcagttt ttgttgaaag gatgtggtgg acaatgaatc
    46741 aaagtaaacg actgtttgtt tcgttttttt ctattttaag tctatttttc atttttccgt
    46801 caatcagcca agcaaaggat tcagcaggag actttggaat caaaccggta tttccagaga
    46861 accaaataga taaagcaatc ggttattttg atttattagt ggcaccggaa caaaatcaga
    46921 cactagaagt aattatcagc aattcttcag atgaagaacg aacatttgag gtctctgtca
    46981 atccggcggt aacgagtgat ggtggaacga tcgattatag tcaaaaaaac ccaacgttag
    47041 atgagacatt gccgtttgat gtccgtgacg ttttgctcat tgcgaaaaag gaaatcaatg
    47101 tatcagcaca tgcagaaaca actgtaccta tagaagtgaa aatcccagca aaaagcttta
    47161 agggacgtgt attagcgggt attcatgttt cacctaaaga agaagctgaa acagaaaatg
    47221 ctacagaagg agcacaaatc aaaaatcgta ttgcttataa tctcgcagtc gttttacaag
    47281 aaagtcaaga gacgatagaa ccggatctga aacttttatc aggagatttg gacgaggtca
    47341 acgcaaagcc aactgttcag cttcgtttcc aaaatcctca gcctaggatt atcagcaatt
    47401 tgatatttac gagcaagatc ttttatgaaa atcagcttta tattgaaaat acgtctaatg
    47461 catttttagt tgcaccaaat tcgaattttc acttgaatct tgatttagct ggtgataaag
    47521 caaaagccgg cgattaccgc gcagaaatca tagcaaaatc aggcgacagt aatgaatggc
    47581 gatttacaca aaactttacg atcaaaaaag aaaaagcaca aaaagtaaat gaaaattccg
    47641 tttttgcagt acaagaacaa tctttcccat ggctttatgc aggattggtt gctgcagttc
    47701 tagtgttatg gttgattttg ctgtttttgt gggtccacaa gaaaaagaaa aaaggagaaa
    47761 caacatgatg aggtgatttc gttgaaaaca tcgatcttat tgggagggat tattgcaagt
    47821 ttgctgttct tcagctggca agagccagtc caagcagata ccaaaagttc aggtacgatt
    47881 acattttatg atctaagtcc aattccgcat ccaagtggtt cagtcaatgg cgcatcaaaa
    47941 cagcagacac agctaccgat ttataaagaa tctaatcaac atggcgtatt cccggagacg
    48001 aatgaacaaa agatccgttt tctttatttg atcattggta tagaattatg gcttcttgcg
    48061 tggttgttgt tatggagaag aaaggaaaag gaggatgaac atgttgctac aaaataaatt
    48121 ttcatttttt cgctccttct tttttacttc gctttttgtt ttaagtatat ttatcttttc
    48181 accttcggca gaagctgttg gagcaacgcc tcctgccatt tcttatgatc catacacgta
    48241 tgaaaatatc gtacctccaa atggtggacg tagtgcagtc aatggcggga agcttgtcca
    48301 actaactggg gctaaccaat tgcctaaatt gactgctact tatttgaacg atggttcaaa
    48361 tgatccttgg aaaggtggca cggtccgttt tcattgggaa agccatgtga aactaacaaa
    48421 tgtacttgat ttggtgaata tcactgcaca aggatttgca tttgtggcga tcatcaattt
    48481 gccggaacat gtatcaagca gtgatgtatt aaaagcaatt gactggaaca acgcctactt
    48541 gtatattgcc ggtgccccca tcaaggttac tagcggaagt ttattatcag caggaagcca
    48601 taccttgcgt tttgggatgg ggtcgtggga tacaaatact ctattgacag atattattgc
    48661 tattattacg cgaggcgaat tcaacgtgga cgatatcccg cttcaatttg atgttacagc
    48721 taatgtcgcg aagatgacag aaaacggaga taaatatgat acgagtccaa acaaaatttt
    48781 gaccaagggt aaattacaac cttctatgtc taaaaaggcc gttttttctg ttgattttta
    48841 tgattcgaat aatattatcc aagatcgtat gttcaattcg attttagctc cagctgcagg
    48901 tagattttat cctaaattaa ctggcggaaa cttgaatcca ggagcatatg cattttctgc
    48961 aagtgcagag ataaatacgt ggaatagtta catctcacca agtgattcaa caggaaaata
    49021 taatacattt gctgatacca cagaatacgt ggatggaaat aaccagaacc ttccaggctc
    49081 taccaatagt cggacattga cgatggggct gactgaagga cgttttgatc agttgccatt
    49141 tagtcgcttt gatcgcgtag tcaactattt tacaggaaaa aacgttacac aaggagcaat
    49201 actgactcat actccagcaa gcacaagtgg attgaatcag acagccaata tcgtgtacag
    49261 tgggaaagat gcgcaaggcg gattgctttc tcctgttgct ttaaaggtat tggagcagta
    49321 caagctagac ggaacggttt taccaacaaa cttaacagcc gatagtcagt ggggaaagct
    49381 cctttcttct gcatcgtacc aagtcacggc caattggtca gcaactgaac taaaaaaggg
    49441 ttcgatccat tacaggcttt attcgaaagg aacccaaaaa ttagcgacag gatggacaga
    49501 tcaacttttc caaacgatcc aatcaaataa cgggggtgta aatacagcta cttctacact
    49561 acctgcattg gcttcagggc aatattattt tgattatcgc ttggtggacg atgtacttac
    49621 taacatgtat ccgtcttttg cgtacaaatg gcagtctgaa caagctggaa tcaccactct
    49681 tcctcaaata acagtggcta attttccatc tgtgagtgca gatagtcagc taaaaaacct
    49741 tagcagacaa cctgagacag gagatcccct gtctgctcta tcaggagata cgattcaaga
    49801 aaatgtgaca ttcacgttaa caaaatctgg ggatgcgata accgacaaaa aaatcacgat
    49861 ctcacttcct gaaaatacta cttatatcaa tggttcctta aaattaaatg gtacaactat
    49921 cgcagatacc ggcattcaac aaggagtttc tgtcccagca gatctattga gcaaaatagg
    49981 agataccatt catcttactt acaactatca attaaatact gtagatactt ctttacaatc
    50041 cgtcagcata ctgacaaagg cagctgtact ttccagcaat attacattag cagatggtgc
    50101 taagcttcct aacccagttg tccaaacatc tgctaaaaca attcttgtcc ccaaacaaga
    50161 attaacatta gttaatgtac cagatgattt tacatttggt aacgatctcc ccaaaccgtt
    50221 gaagacaagt tattatgaat caaaaggcga tttttctttt gatgtgcggg atactcggct
    50281 tccaagcacg tctccttggc aactgacagg tacattgact tctttattta agaatgatca
    50341 aggtcaagaa ttaagcggaa caaaattata cttcaatcat tctggatcaa agcaattgat
    50401 tcagcaaggt caaaacacgt tgatttatga gagtgatgga acagctaaag gagaggttct
    50461 cgttgacttt cccgataccg atggtttact cttagaggta aatagcagta cgaatgctca
    50521 acctggcgca acgtatcaag gcatggtcac atggaaactg acagctgggc caaccagcta
    50581 agtgattaga atttgtatgg atctgtgata actgtcacag atcctctttt tcaaggatga
    50641 gaaggaaact tctgctgtta aaatcagaaa tgcttgaagg agtggacttg gaacttttca
    50701 gtttgcttga aaaaaatgaa caacggcaaa tcaaactttt ccaactttta ttagaggaaa
    50761 aaggagaggt ttccgttaat aacgtactta cccgcttatc agttgatcgg gctaccttga
    50821 aagaagatct gttttattta caagctaact tggaaaatat caaacaattg gaaattgtta
    50881 tccaagaagg gaatatttta ttaatcagac atggaaatat agcagcagtc gatgtttatt
    50941 atcattgtta cttgcgctat tctacaaaat atcagatact tctttatctg ttggaaaatg
    51001 gctcatttga tcggcagaaa cttgtcatgg aacttaatat cagttcggct actttgtctc
    51061 gcagagtgaa ggaattaaat gattctttaa atgagtttca tctgaaaata aaaagcgggc
    51121 ggctgaccgg tccagaaagt caaattcgtt atttttattt ccaattattt tggtttggac
    51181 gtccctatct tgaaaacaaa aaagaataca gtgatttatc aggaaacact tttttagatc
    51241 tgctgcaaaa agagtttagt tttgctatta cagaagaagg acgattgaaa tttgaaatat
    51301 ggctttccat tatgaagaag cgaatgcgct gtacgaaaaa atccaaacca gattatagca
    51361 atcttcctgt tggattgttt caaacgacac aatttttatt ggacctccat acatttttga
    51421 cccgttactt ctttcaatat gcctttcctt ggaatcaaaa tgaaacgttg gttttttatc
    51481 ttttcatgat cagtaacttt acattagata cacataactc gcatgtccag gagtttttga
    51541 taagcgaaaa atacaaagac gacgtagtag ctcaattgaa tcgaacgttt aaacaatcga
    51601 tagaagaata tttctctttt tatgtttttc cagaagagat ttcgcaaaag attgcgttga
    51661 cagtgatgca aaatcacgct cattttgcct attttagagg atggatgaat atttttggcc
    51721 aagaaggatt aaaacagcgg ttaactgaag tagaagataa acaattcctg gttttatgtg
    51781 aaagattggt cagacaaaca tttgctattg ttgattttac tgaagtagac gaggaaaatg
    51841 cccgctttga attatttgct cgctatgcat ctattttacg gacgctgatc aagcagttag
    51901 aattacgtct gcaaattgct tatgatcttc cttatgaaag gctaatggcg gatatcgtga
    51961 aagaaatcat ccaggaacaa ttgctgaata cgatcaaata tgatttagtc gaatacgaga
    52021 aagaaaagca gtatgatatc attttgacta gccaattgaa agagtacccg tcacagaagt
    52081 ccgctaaagt ttttgtattc actagcaacg aatataatta tgatttcccc tatttgaacc
    52141 aatttttaaa agaatgttat ttagaaaaat tgaatttaag gatgaataaa acatgaaaat
    52201 cgtgatcatt ggtgcttccc atgcaggcat cacagctgcc ctaaatctaa gaaaacttca
    52261 accgaaagca gaagttttac tgatcgatga agatcataaa gatggactag gctatgtttc
    52321 aaatggaatc aacctttatt taaaaggaaa gatacgtagt ttagctgaag ttgcccataa
    52381 catgcggact cttcaagaaa gtggggcaaa actgatcacc gaatggagag taactgaact
    52441 tgatccagac aaacatcagc tcatcctcgc atccaaagaa ggaaaaaacg agaccataac
    52501 ctatgataaa ttaatcgtag cgacaggttc ctctcctgtc accttgtata aacaaattga
    52561 agctgaaaat gtctacacct ataaaaatct agtgcaatct aagcaagtac ttgccgcttt
    52621 aaaagaagca aaagaagtag tgatcttcgg tgctggttac attggattgg aattagcaga
    52681 tgctttgaga aataaaggat acattattca tctagttgat tatatgccga atgttttatc
    52741 aagatatttc gataaggata tgatcaacag ttttcaaaat cagctgcaaa ctaagcaaat
    52801 aaatttttat ccaaatgaat tcttgatcga ttggaaaaaa tcagaagaga aagtagtatc
    52861 cgtacaactt ctgtctcaaa caataaaggc agatatggtc attttttctg cccaaactcg
    52921 tcctaatacg acattgttaa aagaaaaagt ggctttgtat gaggatgaga ctgtgatagt
    52981 caatgaatat ctgcagacaa gtgatccgga catctatgcg attggagata tcgtcccagt
    53041 cagttttgac aaaaataaac ggcatctctt tttaccttta gttactcgag ctgtacatat
    53101 ggcaagagca gtagcgctaa ctttgagtgg tcagccaacc gcttacgacc tcagacaaaa
    53161 gataacagca gcagtcatta tagactactt tttaggaact gtagggctga cagaagatga
    53221 agcgccattc ttggaacaaa ataccggttc gtgttcagga gaatttgatc tatttccaca
    53281 atacgacgaa gaaaacaaaa cagtaaatgc aaagctgatc taccatccgg atacattaga
    53341 aataattggc ggacagctga taagccaaga attcttatta tcagatttga acctgcttgc
    53401 ggatatcgtg aaacacaaaa ctacgattcc acagctagca gttgaggatt ttggatttct
    53461 gtctgaatac acctctcgtt ttcactacct caatgagtta gcttttaaag tactagctaa
    53521 gagagcaaat agcaatagag tagataaaag actgtgagaa atatcacggt cttttattgt
    53581 gtggggatat aataaatatt aatcaatcga gaataaaaaa ttcatataat gaaatagcgg
    53641 aagaattttt tgagcataat attcgccaaa aatttatcaa gtgataaaat tgttgtataa
    53701 aatgattatt tcaggaggag gagttaaatg tttttagcat taaatgaaat tatgcattca
    53761 aagttacgtt atgcgttagt tgcaggggtg atgtttttga tcgcctacct ggtatttttt
    53821 ttgactgggt tagcttatgg cttagctcaa gataatcgaa cagcagtcga caagtgggag
    53881 gcagattcga ttgtgctttc gaaagatgca aattcaaatc tagggatgtc catgattact
    53941 aagaagatag cagaagaagt cgaaggtggg aaagtggcgt atcttgccca aacacctgga
    54001 gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca atgttagctt cttcggtatc
    54061 gataaaaatc aattcatcat gcccaatctt gttgaaggaa aagcttttga taacgatgat
    54121 gaggcagtag gcgatattag tttgaaagaa gaatatggtc tagccatagg agatacagtt
    54181 aagttatctg gtagtgacaa gacgtttaaa ctaactggct ttacggatca tgccaaattc
    54241 aatgtctcac cagttctcta tacaaccatc aatgcttacc aagaaatccg cttcgaaaaa
    54301 gaagatacaa gtgaaaatgc acggattaac gcaatagttg tcagaggtaa aatcagtaat
    54361 ttacctgaag atttagaaca gatcaaaatt tctaaattca tcaatgaatt acctggatat
    54421 aatgcgcaag tattgacttt tggatttatg atcggatttt tgatcgttat tgcggctatt
    54481 gtgatcggga ttttcattta tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg
    54541 aaagcacaag ggatcacagg aggatttatt gcgcgttctg tagtggcaca aacattcatt
    54601 ttatcatttg ttggtattct tcttggtcta ttaggaacag tgggaacatc ccttgttcta
    54661 cctgatgcgg ttccattcca aagcaattgg ctgttttttg gcgtcattag cttgttgatg
    54721 ttagtagtag ctgtgttagg cgcactattt tctgttcgga caatcgtaaa aatcgatccg
    54781 ttgaaggcaa ttggataaga aggaggaaga acatgaaagc aatagaattt aaagaagtag
    54841 aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca accatgagta
    54901 tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt
    54961 tgactttggc aggcggattg cagacgccta gcagtggctc tgtatttatc aatgatcaag
    55021 aattcagtaa tcaaaaagaa agcgaacgcg ttaaactacg ttttaaagaa attggcttta
    55081 ttttgcaagc ttccaatctg gttccttttc tgacagtaaa aaagcagtta ttattagtag
    55141 ataagattaa acatgaacat cgtcaaaaag aagcaaacga attatttgag cagcttggcg
    55201 tggataagtt gatcaacaag tatccagaag aactatctgg tggagaacgg caaagggtcg
    55261 caatcgctcg tgcattgtac aatgatccgt ccattattct agcagatgaa ccaactgcta
    55321 gtttggattc agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca
    55381 aaaataaagc aacgatcatg gttactcacg atacacggct gattgacaaa tgcgatagaa
    55441 tatttatgat ggaagatggt gtattgaaag aaaaagaata accaaaaagc aggtggaaaa
    55501 atggtccatc gatacaacaa agatacacag caaaaaattt tgaaagaagc gactgacttg
    55561 tttatgtcca agggatatct cggcacttca acaagagaaa tcgcacaaaa agttgggatc
    55621 acccagccca atctctatca ttatttcggt gataaagaaa aactatatac ggcagtttta
    55681 gaaaaccact taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg
    55741 ggatttcaag taaccttgac aaaaatggcg cagtttttga ttgagacaca tctggtcgat
    55801 ttgtttctga tgcttcacga tcttgaaagc aatctttcaa aggaaacaag agatcatctt
    55861 ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa
    55921 tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta
    55981 gcaccttaca tcactcggtc gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg
    56041 atcgatttat atttgaatgg tgtattagga gaagcgtaaa gctgttatat agggagaaaa
    56101 tagttaggtg aagaagcaag atatgacgaa gatcgttata tcttgcttct ttatataggt
    56161 agtttttcat aaagacaaat ttgttattct ctcatgatta tcagggtata tttttattta
    56221 ttatgtcggt gttccaggaa gtgttttaat ttaataacga agaaattaat tgtttgtaga
    56281 atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa tagtcaaaag gagatttcag
    56341 gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt tattttctac ggttatatct
    56401 tttacagccc cttttttgct tacttaagct gattttaagt tttggccctt agtctggatc
    56461 tacgaggtga gagtcaagaa tgaaaaaaat aattggagct tttgtatttg caatcattat
    56521 cattattatc tgttttcaaa tagatgtttt tggtcaaaaa aagatagttg gacaaacggt
    56581 cagcagtacg aacagtacgg ttacaaagac aaaggaatca tttgatgata cagaacaaga
    56641 gacattaatc aaaatcggaa aaaatgaccg tacactatat acagatgact cgctaactac
    56701 cccacttgca actataaacg gcggagagct ggcggagttt ctttctgaaa caaaaaatgc
    56761 ttatcagatc aagacaaatg atggctatac tggatattta gccctcgaag atggaaccaa
    56821 agtcacaaag aatatacaaa caaaaccgga agagctatct gatgcagtga ttgtattaga
    56881 tcctgggcat ggcggaaatg atactggggc attaagcaat gatgaacaaa cagaagaaaa
    56941 agatataaca ttaagtacag ccataaaagt aaaaaaggcg ctagaggatg ctggagcaac
    57001 ggtttatttg acccatacta cagatgaatt agtacagctt ggagatatat gcgactacag
    57061 tgaagaaaaa aaggcagatg tctttatcag tttacacgca gactctacgg aatatgcgaa
    57121 tgaagcaact ggaatcacga catattatta ctatggacaa gaggaaacat tagcacagac
    57181 aattgctgac tccttcactg atctgcctct ggattcaaga ggaatctcca ctgggaacta
    57241 tcaagttctt cgagaaaatc ttcaaccttc tattttaatc gagatgggtt atatgaataa
    57301 tgatagtgat ttaaaggaat tagtaacaga cagttatcag cagcagattg ctgcaagttt
    57361 gacacaagca ttgaccactt attttcagta ataaataagc ccagccaaac aaaaatagct
    57421 cctcatattt ttcgtttggc cgggcttatt atcgcagatg caagcttttg aacaccgttt
    57481 atttttttag tagcttcaaa gctttttctg tatttgcaaa gtgaagtttg gcatattttt
    57541 caagcatagg catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc
    57601 cagctcctga tggaagggtg attccagctt cagctgatgc tttgtgtaac tcttccaaaa
    57661 agctggcacg actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag
    57721 taacgaagta cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat
    57781 ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt
    57841 ttagcagcaa atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt
    57901 ttggttggat ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt
    57961 tttttaagat gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa
    58021 caccaagttt acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta
    58081 gcggaccgaa atagctgcca tttccgacct catcactacc taaaacggat aattgactga
    58141 tattttttgg caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc
    58201 cccatttatc agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat
    58261 aagcggtaat ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat
    58321 ttaacaaata agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga
    58381 taatgacgtt gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac
    58441 gacttttcgt tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat
    58501 agttaagtat gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact
    58561 aaataaaact ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta
    58621 caaagcggtg attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat
    58681 ggatatcgta acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca
    58741 aatggataat gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa
    58801 gaaacaagaa agaatcttgg agctggaaga agaaacagca gaattaaaga aaaaaatgat
    58861 caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg
    58921 ggaagtattg aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca
    58981 acagatttta aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc
    59041 ttttatcaaa ataagaaaaa tttaaaaaaa gttagaagag aaaggaacgt agcatgctga
    59101 gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg
    59161 ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt
    59221 acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc acgccaacat
    59281 caaagttagc attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag
    59341 gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt
    59401 ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca
    59461 tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg
    59521 ttccattaga tgtggtccaa aatcaattcg gcgagcatag tttcgctcgc tttatcgttg
    59581 agagaacgcc atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt
    59641 aaggaaaata gtgctggaaa atatttcgtt ttttgtacag agtagattat ttaagaaggc
    59701 tgcgtagcgt atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga
    59761 ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg
    59821 tagtgactgc acaaggaaaa gaagaactgg cagaattggt accagtgagc gaggaacaaa
    59881 cgatcacaaa ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg
    59941 ggatccctat acctaaactt gaaaatatcc gtccccatat gaaacggatt gagatcggag
    60001 cagacttgaa tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat
    60061 tgaatcgttt cattgatgat ctttatgaca gtgaaatcga atttgcacgt ttgtatatgt
    60121 gggcagatca gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg
    60181 aagacggacg cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc
    60241 ggcgaagtga acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa
    60301 agtatttaag tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac
    60361 aagaataccg cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggacagactt
    60421 tatttattga accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg
    60481 ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc
    60541 gtcaagaaat catccacaat gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag
    60601 cacgatttgg taaagaagta aaagctatcg tgccgggaat cagtgaagac aatcatgtcg
    60661 tcttgaaaca agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca
    60721 caatcggaaa agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga
    60781 tcacgttgaa gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg
    60841 caggtgaaga aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac
    60901 agtcgattga gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt
    60961 tatcaaaagt caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc
    61021 cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt
    61081 atgtgatggc aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata
    61141 cgattaatgc cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga
    61201 ttggcgttcc tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag
    61261 aagttattga tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga
    61321 tcacagattt agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt
    61381 ttgtttctga ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg
    61441 aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg
    61501 cggaagaaaa agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc
    61561 aaggaaatgt gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc
    61621 aagaagaaac tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga
    61681 aaccgggaga cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa
    61741 acgggaatca gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc
    61801 tgacgcctgt tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg
    61861 aatctagtag tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc
    61921 taagtgaggt agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta
    61981 ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc
    62041 atcggagcgt caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa
    62101 ttgttaaatt taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga
    62161 aatgctatac tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac
    62221 agataagaca tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac
    62281 ttggtgtggt ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga
    62341 tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca
    62401 attcggtatc atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa
    62461 agctgttggt gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa
    62521 acttacagca aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg
    62581 cggttgagtt aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg
    62641 attgtttttt tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga
    62701 tgaacgaacg aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga
    62761 tgaaagataa aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg
    62821 tccgctcata ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg
    62881 aagactttga atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc
    62941 tgataaagaa aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt
    63001 tccttaagat aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag
    63061 acaaagcact gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga
    63121 tcctcgatcg attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc
    63181 tttactatca tttgggacaa tgtctgtgtc cttattattt cgacgtagat ccgaaagtct
    63241 atacgaaaat cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt
    63301 cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag
    63361 aataccgaga ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata
    63421 ctgatttact ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc
    63481 aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct
    63541 ataacgaagc agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc
    63601 atttcattcc taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt
    63661 tgttagctac gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg
    63721 cgagtaagaa tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg
    63781 aacggacgat cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac
    63841 gtatcgagtc ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg
    63901 tattcattga tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca
    63961 acggaccgga tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga
    64021 ttcgcgaagg gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag
    64081 atgtggcaaa agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg
    64141 caaaaaacga taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac
    64201 cgcttgagcg aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc
    64261 gattcgcaat cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag
    64321 atgaaattga agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt
    64381 tgaaaaatat cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg
    64441 tcgcacaaaa cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa
    64501 agtaaagtgt cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat
    64561 aataaatcgc ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag
    64621 aaatgttaga agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg
    64681 tctctttcca aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga
    64741 agacaacgac ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat
    64801 ggaatggcca tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac
    64861 ggggacttta tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag
    64921 ggaaatcaag aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa
    64981 aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc
    65041 tgatcgccac gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt
    65101 tagatccggt aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt
    65161 catgtgtcat tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga
    65221 tcatgttgcg aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat
    65281 ttggccgtac gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg
    65341 aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc
    65401 ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta
    65461 accagcaacc gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt
    65521 aagttttgga ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg
    65581 atcatgatat tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct
    65641 caacaaaata gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag
    65701 caaatgagcc aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa
    65761 gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat
    65821 gataagataa atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg
    65881 atacaacaac aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca
    65941 ccagaagaag tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt
    66001 gattcaaaag gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt
    66061 tatgtagcaa cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa
    66121 gagatcgctt ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca
    66181 caaaagcatt tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca
    66241 ttatacggta ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa
    66301 aatatattag acggtatctc attggataac ttgattggac cattcttgtg gtattctctt
    66361 ctattcatga tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg
    66421 gtcaacaaag cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc
    66481 ggatacatgc ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc
    66541 acttcgtata ttccattctt atcttcatat attatgccgg ttcgcttagc aaatgagaca
    66601 gtcggtatgg gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg
    66661 atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta
    66721 tggagttcat tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac
    66781 aatcctaaaa tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac
    66841 ctattttatt tattttacta tattggatat atattcattg aaaatagtgt ataacaactt
    66901 tataatattg agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact
    66961 ttcagaaaaa ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat
    67021 tttgcagctt ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc
    67081 ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt
    67141 ttctgaacaa atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga
    67201 atatgcatta acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt
    67261 gatcgatctt ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt
    67321 gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg
    67381 aaaaaaagac tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg aagaagaaaa
    67441 atttttaacg aaacttgcac agacgatcca aaaatctgtc atcgatcatt tgcatatcgt
    67501 tggagatatt tttgaccgag gaactcaagc agccaaagtg atggatcgaa tcatggattt
    67561 ttcttctgta gatatccaat ggggaaatca tgatattttg tggattggtg cgtattgcgg
    67621 ctctgaggca tgtttactga cacttttacg gattgcagca cgctataatt atttatttga
    67681 attagagaaa gaatatgggt tgaatttacg tccattgttt tcttttgctc atcaaactta
    67741 tcagaagaat ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa gagagcaaga
    67801 agaattagaa aaaatccatc aggcactttc tatccttcaa ttcaaatcag aacatcagtt
    67861 attggatcgt cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa aaatcgacta
    67921 tgaagcatca acgattgatt tagaaggtca gctctatgag ttgaaaggaa cttgttttca
    67981 aacgatccag ccagatgatc caaaaaaatt cttggaagag gaacaaaaag tgattgatag
    68041 tttgatgtat tcattccagc actctttgag aatgcaaaaa catatgacgt ttttgatcga
    68101 aaaaggggga atgtatctca cgaacaatca gcatgtgctg tttcatggct gtatacctgt
    68161 agacgagaag ggacagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact
    68221 gctgggcttt tttgaaaaac agataactga aagcgcaaaa gatctgactg caaaagaaga
    68281 tttagcaacc gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt tattcggcaa
    68341 gagtaaaatg gcgacttttg aacgttattt tctcacagag cctcatacgc acgtcgagaa
    68401 aagcaatcct tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttagcaga
    68461 attcgacaca aaagaagaag ggtcagcaat cattaatggg cacacgccag taaaagtaaa
    68521 aaaaggggag tcaccgatca aagcaaatgg aaaagtattt gtcattgacg gaggtttatc
    68581 caaagcttat caaaaaacga caggaattgc cggttattca ctattatgca attcttatgg
    68641 tttccaaatc gtgacacatt atccgttttt gcctattgaa caacaatttg caaaaaaatc
    68701 cgaccaaaca aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac tcaatcgtga
    68761 tacaacaaaa ggtatggaac tgcagcagca gattacccag ctcaaaaggc tgttggacta
    68821 tcgaaaggac gattgagaag aattccagtg aacattgttc catagactgg agactgagac
    68881 aaaagtcgtg tcgcagtctc tttttccgga taaacggtgt tcttctgcca actcctcggt
    68941 cacaagtcac actattcaac aaacatgaaa agctgttttt tgaaattgct ccttgtgact
    69001 cagagttgaa cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gccagtttct
    69061 cggtattaag ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa tttttcttaa
    69121 tactcgaaac taaacagctt tttcacaacc ccctcagaaa gtctattaaa attcattcac
    69181 aaagaaatcg aataagcgct gttcagatgg atgtacacgg tgggtaagtt ccggatgcca
    69241 ttgaacaccg aggattttat gcatatcatc tttgctttga accgcttcaa tgagtccatc
    69301 gttagaaaaa gcgatggctt ttaataaagg tgaaagatct ttgactgcct gatgatggta
    69361 agaattcaca aaatatggat cggggagaag gttgcttaac aaactgtctg aaacaatctt
    69421 gacttcgtga gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta ctgaccactt
    69481 tggatataat gaaagatctt gataaagtgt accttccaaa acgacattca atagttggag
    69541 tccccggcag actgcaaaaa ttggttttct ttgtttcaat gcttccaaga tcagtgcctg
    69601 ttcaaattgg tctcgttgaa tatttgtttc cgttagcttt ggatgaggat cttccatgta
    69661 aagttgtgga ctgacatctt gaccacctgc aagaataagt ttgtcgattt gtccaatcag
    69721 ttgaggagct gaatctggag acatgactgg cagaataaga ggcaatcctt gagcatctaa
    69781 aactgcatcc acgaatcctt gcggtgtata gctcacttga tttccttgga aagtatctgt
    69841 tgctcgaatc agttgatttc cggcaatgcc gatgattggt ttcatttgta ttccccctaa
    69901 agtagactta ttgtaacaag gatttaaaaa aaattaaact atgtaagact tgcccctata
    69961 cttatctgtt cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa attcatgacg
    70021 atagttgaaa atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg
    70081 gcgtaagctt caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc
    70141 tgcctatact aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc
    70201 tcttttttaa atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa
    70261 gcagaagaag aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa gatctatgca
    70321 acagaaaaag agcaggaatt ggaaacgatc caaacaaact atcgacaaca actaaaacag
    70381 ttggtggaag aaaaagaaca atttgaaata caagaattag aaaaagaaaa aatgaaattg
    70441 aaagcagaag cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa gcatcagagt
    70501 caagcgatcg gtgcgatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga
    70561 aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg
    70621 aatccaaaac gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtgga
    70681 gaagtatttt ccagatacca cgattattga taaagaagca aaattaagcg ctttaggtaa
    70741 tcggttgaaa gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca
    70801 aaagaaactc catttgaaac gtcaagaact ttccttacaa gatttggaag ccaattattc
    70861 cgaagaaaat ttcataaata aattgaaaga agtccttgat ttaaaagaaa agtgggaaca
    70921 actaactgaa gaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca
    70981 gctggatatt gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt
    71041 gagtgcagca aattttgaaa gtttcaagga agaactgagt aagcttgatg acgtttatat
    71101 ggaagaaata gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc
    71161 agttgctgtt atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta
    71221 tgaacaaaat cctaaagaaa aactgcaaga gaccaagcag caattatcca ttgttcacga
    71281 gcagcaaaag aagataacga cagcgttagg tcagtgcagt ggctatatcc aagaatttga
    71341 atgggtagaa gaagtcacac ttgccgttgc agaacgagaa aaaataaaag aacggtttat
    71401 ccatgcgact tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt
    71461 gctgcatgtg ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt tcgaaacacc
    71521 aaactcagaa gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttgtcga
    71581 accttttgaa ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac
    71641 accttggatg acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta
    71701 tggtttgctg atgctcattg ggacaatact agctcaaaaa ctacttgttc taccacgcgg
    71761 gatgaaacgt ttcgtaaaat tctttgagat tctctcgata ccatcgatca tctggggatt
    71821 gatctacagt tcatttttcg gacagtcatt accaaaagaa ctgtttggca tccgcttgcc
    71881 tttcccaatc ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt
    71941 cggtctgata cagattttgg ttggattatt tgtggcagca aaggaaaata tcaggagaaa
    72001 agattacttg agtgctgtca gtgatggatt tgcctggcaa gggatcctaa ttgggatcgt
    72061 tattgcgttg gttggtgcca tgttgataaa aaatacagca tttgtttacc ttggcgctgg
    72121 gctatcgatc attgctgcct tatgtatctt gatcgttccg attatccaaa cgccgtctaa
    72181 agtcaaaggt gctgccaaag gtgcgtataa tctttacgga ctgaccggct atatcggtga
    72241 tttagtcagt tatacacgtt tgatggcatt gggtatttca ggcggaagta ttggagcggc
    72301 attcaatatg ctagttgctt tcatgccgcc agctgctcga ttcagtgtag ggatcctttt
    72361 gatcatcgct ttacacgcac tgaatatgtt tttgacactg ctaagtgcct atgtccatgg
    72421 cgcacgattg cagtatgtcg aattttttgg gaagttttat acaggaggcg gacgtgcctt
    72481 tgatccgctg aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa aaaacaataa
    72541 ataattggag gatttttata taatgatgga ttacttaatt agtcaaaatg gtggaatggt
    72601 atttgcggta ttagcgatgg caacagcgac gattttttca gggatcggtt cagctaaagg
    72661 tgttggaatg acaggtgagg cagcagcagc attgacaaca agtcaacccg aaaaattcgg
    72721 gcaggctctg attctgcaat tgcttccagg tacgcaaggt ttgtatggat tcgttattgc
    72781 ctttttgatt tttatcaact taggaaatga catgtcagtt gtacaaggac tgaactttct
    72841 aggtgcttct ctgccgattg cttttacagg cttgttttca ggtattgccc aaggaaaagt
    72901 cgcagcggca ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat
    72961 ttttgctgcg atggttgaaa catacgcgat tttaggtttc gttatttcct tcctattagt
    73021 gttgaatgcc taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct
    73081 cagatgaacg aagcagcaga gcaggaaaga gcggcgttag aacaagaaga gcgaatgaag
    73141 atcgatcaaa actttgaaca aaaacgtacg caagttgaaa cagaacatca aaaacaaaaa
    73201 gaaaaacaaa tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg ccagcaagta
    73261 gaagtacgac aagaaaattt gaatgcgaag caggaatttt taagacgatt gtttgcagat
    73321 gcggtaactg aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat caaaaatgca
    73381 ttgtattcat tacctttgac aggaaaagta gcattcattg ctggagaaaa atcagcagcc
    73441 tatctttctc agacgttgtt ggatgaatgg aacaatgaac ttccctttat gatggtttta
    73501 agtgatgaaa ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat
    73561 aattttcttt tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc
    73621 aatcaattat tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga
    73681 ttcgcggaag agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa
    73741 attctgtcga agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg
    73801 acggatatga atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct
    73861 ggttaaagga atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgttatactt
    73921 tccataatct gaaggttttg acaaaagctg agatgacaaa tcaaaatctg gaccatttgt
    73981 atatcgacga tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt
    74041 caaatgaact tcctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag
    74101 agtctaccat tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc
    74161 gacggcttgc cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg
    74221 acttgaccaa tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca
    74281 tgacgacggt actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag
    74341 ataaagatat ggaagattac acgaattttc tattagagac agattatgct gattttctgc
    74401 gccctgtcat ccgtgaggga aatattgatc tcgtaaagct tgaacagttg aaagatgatt
    74461 atctaagttc attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag
    74521 cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa
    74581 gaagccattt cgaatcagaa acaatcagaa agcgggtgag acaagtctat gacgcataag
    74641 atcggtgtag ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt
    74701 cgttatggta caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag
    74761 tatggcgtga tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt
    74821 tacaaggaac aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg
    74881 atagggctgg aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag
    74941 tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt
    75001 gtaatggctg agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta
    75061 ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa
    75121 gagacttctg gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt
    75181 gaattaggac cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc
    75241 tttatggaaa tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac
    75301 catgagaaaa aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga
    75361 gatgttatcg ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat
    75421 gggataaaag gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta
    75481 tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta
    75541 agacgtagtc ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag
    75601 cgagtgattg atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct
    75661 tttggtgcag ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg
    75721 gttgtctatg tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc
    75781 cctgaattga ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct
    75841 aatacatcaa acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt
    75901 gcggaatatt tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga
    75961 tgggcagaag cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt
    76021 tatccagctt atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt
    76081 gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct
    76141 ggaggggata tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg
    76201 ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc
    76261 tattctcttt attcaacaga agtagggaag tatatggatc atatccttca agctgactgg
    76321 gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc
    76381 gtgcgtttgg tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca
    76441 aaatccattc gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact
    76501 tcaagagaga aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc
    76561 cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt
    76621 gaaaggatcg gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt
    76681 attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat
    76741 taaagaatat cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc
    76801 tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg
    76861 acaagtattg gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg
    76921 tatcaattta cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc
    76981 ggaagacatg atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga
    77041 gatcctgcct gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga
    77101 ttatccagat gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt
    77161 tcgtgggcaa aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc
    77221 tcagatcgcg cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc
    77281 tgctattgga atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg
    77341 cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat
    77401 cgctactcct cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca
    77461 tgttttggtt atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc
    77521 agcacggcga gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc
    77581 tactttattc gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc
    77641 gattctaaca atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat
    77701 tacagaagga cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat
    77761 tgatgtactc ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag
    77821 agaagaccat gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc
    77881 aaaagaatta gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc
    77941 gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc
    78001 gattactgaa acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt
    78061 aaaacgaatc aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat
    78121 ggcacgattg aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac
    78181 aactgccaca agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt
    78241 catcctgttg attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga
    78301 agcaatgtct gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt
    78361 atttgttttg ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt
    78421 aaaagtacct gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata
    78481 cgggtatttg aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct
    78541 tccaaaactt ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat
    78601 cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga
    78661 aacgatctat tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt
    78721 gatcaaagta aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa
    78781 acgatgatta aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca
    78841 ttaggatttt ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt
    78901 aatagtgggc atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt
    78961 gtaacgggat tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg
    79021 atcatgcttt tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt
    79081 gcattgataa agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat
    79141 ctagaagaag tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtg
    79201 atccaattga ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg
    79261 aaaggtatct ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat
    79321 ttgttaggag acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct
    79381 gctttgatca tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat
    79441 cagaaaaaga agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta
    79501 ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg
    79561 aatatgtttc aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga
    79621 tattattcag ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg
    79681 atgtacatcg gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt
    79741 gtgttgctga tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc
    79801 cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg
    79861 ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc
    79921 atcgaatata ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga
    79981 ttgacgccag acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga
    80041 cgagttggtg tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca
    80101 aaatacaaat atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc
    80161 tagtgattta gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt
    80221 gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct
    80281 tttttattga aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca
    80341 tattttttta ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta
    80401 gaggataggg aaattcgaac gaatgcatct ggttgcgtac gtgtagtaaa gcgtctggaa
    80461 tgataaagtc ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca
    80521 ggatcaactg gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa
    80581 agagaatcaa aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta
    80641 tttgcttcat ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc
    80701 acatttatat taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga
    80761 taagcaactc gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg
    80821 ttaggtaata aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat
    80881 gaatggatat catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc
    80941 tctcgtcttt tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag
    81001 acaccatgaa tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc
    81061 tcgtcaaaat caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta
    81121 gcgtatgtat atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg
    81181 atagccaatc cattttgaac accggaaaca atagatagag attctaagga cggaatgact
    81241 ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct
    81301 tggtaattaa gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca
    81361 acgaatttat tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt
    81421 acttcattgg tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt
    81481 ccaatattcg tatacagcaa acctttctca attccatatt tataagcctt gccaacggta
    81541 gtaaaattta aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc
    81601 tgtaatttat tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga
    81661 cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata
    81721 ggcattttct ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat
    81781 tttagaagtt aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa
    81841 gtagtagctt cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat
    81901 tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa
    81961 ggcaaaaata tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca
    82021 aaagtcatta ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag
    82081 attttcttgt ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc
    82141 acctggattc gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat
    82201 gcagaaacac ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt
    82261 tctaccattc gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat
    82321 acttttatcg ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca
    82381 gtagaagatg cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca
    82441 tttcttcaaa aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact
    82501 cctgatacaa gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat
    82561 tactcagctg ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta
    82621 gaggaatctt gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat
    82681 aaaggagaat cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt
    82741 tgtgcagaat atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt
    82801 acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc
    82861 tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg
    82921 aaacagcatt tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc
    82981 gagaaatcga tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt
    83041 acaaaatacc tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa
    83101 gaattaaaga ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat
    83161 tgtctgaata tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt
    83221 tatttttcgt attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta
    83281 tcaaaaattt tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact
    83341 ttgtttctag aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag
    83401 cacattctaa tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt
    83461 atcaaatgtc atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa
    83521 taggaggaaa agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg
    83581 aattaaaaat aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag
    83641 acgcaaactt tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt
    83701 ttttattcaa tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta
    83761 taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag
    83821 tcagtaatct caagaccagc agctttatgt tgctcgtgta atttttctag atcatcagct
    83881 gctatagcga tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa
    83941 gtcaactcta attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt
    84001 tctggaaaat cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt
    84061 gatgcttcaa gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc
    84121 cttttccttt tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt
    84181 catgagaaat ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc
    84241 atggataaac caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca
    84301 gcatatattg tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct
    84361 ttttttcttc ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt
    84421 gagctgatag aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat
    84481 gagtattttt actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt
    84541 gctgattctt ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg
    84601 agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga
    84661 tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt
    84721 tatttattac agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga
    84781 agtgatgaca tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag
    84841 accagcatat tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc
    84901 ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt
    84961 atttagtact tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt
    85021 ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt
    85081 gacgatgttc atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac
    85141 taaacttgct tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt
    85201 gtcatcgatc gaatggagat gactgccgac acggcgtgcc atcacttcga tctctaggaa
    85261 atcttcatgt gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag
    85321 tgccccgtta taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg
    85381 ttcctggaca cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac
    85441 agcagcggca atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt
    85501 accatcgatg tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt
    85561 tatttcaatc aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa
    85621 taaatcataa tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc
    85681 atctccttgt tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat
    85741 caaagttccg tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg
    85801 ataaaaatca taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata
    85861 tccgaccttt tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga
    85921 aaaagtagca gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat
    85981 aggatcagca gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt
    86041 gaggtattct tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag
    86101 ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa
    86161 aattacttcc ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg
    86221 gctaacgata taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc
    86281 ataaggacga atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt
    86341 gaaataagca tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat
    86401 ttggacaact tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa
    86461 accttcttct actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc
    86521 tacagtgatg gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc
    86581 atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca
    86641 gcctccgttg cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct
    86701 tgaaatctca taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt
    86761 gaaagaagaa gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat
    86821 caaatctaag attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac
    86881 tttttccata ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt
    86941 tattacgttg ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt
    87001 ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg
    87061 caaatgcttc tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg
    87121 ccattgtagc aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag
    87181 attgataaaa tcgcttcatg acttgcccgc gtttttcctg attaccgttg acacttaagt
    87241 acatcataac agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc
    87301 catggatgct taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta
    87361 tcggtgcctt tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta
    87421 tggttatttt ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat
    87481 ctgatacgat ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatctttca
    87541 gtgtttggat cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg
    87601 tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta
    87661 atatatctgt taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc
    87721 gaatcgcagg gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat
    87781 actctaaaat caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt
    87841 tgacaaaaca aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat
    87901 ggaattatca caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg
    87961 ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct
    88021 gtacatacgt tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa
    88081 attagttcca gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa
    88141 atttggctat aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat
    88201 tgctactttt acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt
    88261 tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt
    88321 ggattttaca gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat
    88381 aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa
    88441 caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt
    88501 catggatttt tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga
    88561 aattcgtgat tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc
    88621 attaggggat aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga
    88681 agtaaaccat ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa
    88741 agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga
    88801 agatacagat ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga
    88861 tgaagaaatc gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga
    88921 tgatgaagaa gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga
    88981 tgatgatgaa gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt
    89041 ttcttacgag aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga
    89101 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
    89161 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
    89221 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
    89281 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
    89341 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
    89401 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
    89461 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
    89521 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
    89581 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
    89641 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
    89701 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
    89761 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
    89821 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
    89881 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
    89941 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
    90001 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
    90061 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
    90121 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
    90181 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
    90241 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
    90301 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
    90361 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
    90421 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
    90481 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
    90541 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
    90601 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
    90661 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
    90721 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
    90781 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
    90841 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
    90901 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
    90961 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
    91021 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
    91081 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
    91141 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
    91201 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
    91261 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
    91321 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
    91381 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
    91441 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
    91501 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
    91561 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
    91621 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
    91681 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
    91741 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
    91801 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
    91861 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
    91921 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
    91981 gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
    92041 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
    92101 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
    92161 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
    92221 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
    92281 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
    92341 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
    92401 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
    92461 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
    92521 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
    92581 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
    92641 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
    92701 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
    92761 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
    92821 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
    92881 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
    92941 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
    93001 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
    93061 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
    93121 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
    93181 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
    93241 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
    93301 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
    93361 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
    93421 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg
    93481 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
    93541 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
    93601 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
    93661 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
    93721 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
    93781 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
    93841 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
    93901 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
    93961 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
    94021 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
    94081 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
    94141 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
    94201 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
    94261 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
    94321 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
    94381 tcatggcaat cgcgggcgga aaatcaaaag ctaaagcaat tcgagcttat atgaaaaatg
    94441 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
    94501 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
    94561 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
    94621 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
    94681 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
    94741 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
    94801 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
    94861 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
    94921 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
    94981 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
    95041 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
    95101 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
    95161 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
    95221 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
    95281 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
    95341 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
    95401 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
    95461 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
    95521 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
    95581 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
    95641 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
    95701 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
    95761 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
    95821 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
    95881 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
    95941 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
    96001 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
    96061 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
    96121 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
    96181 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
    96241 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
    96301 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
    96361 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
    96421 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
    96481 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
    96541 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
    96601 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
    96661 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
    96721 agactcagcc gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
    96781 aggtggcggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
    96841 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgaac aaaaggtgaa
    96901 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
    96961 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
    97021 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
    97081 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
    97141 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
    97201 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
    97261 tccatgaaac agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga
    97321 caccaatcct atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat
    97381 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacag gtgtcaaaca
    97441 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
    97501 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
    97561 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
    97621 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
    97681 cattcttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
    97741 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
    97801 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
    97861 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
    97921 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
    97981 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
    98041 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
    98101 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
    98161 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
    98221 cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
    98281 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
    98341 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
    98401 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
    98461 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
    98521 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
    98581 gaaaaaggtg tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg
    98641 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta
    98701 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
    98761 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
    98821 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
    98881 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
    98941 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa
    99001 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
    99061 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    99121 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    99181 acttagttaa gtaatcgaac tctctacttg ctgaaatatc agcaggtaga gagttttttt
    99241 gcaactagct tctgtatatg atgtttacaa agaatggtac aagaaaagat catccttgaa
    99301 aaaggaaagc taacaataac tagcatcata taagagcttt taaggataga acagtagaat
    99361 aggaatctat gagcaagtcc tctcgtataa ataaaatagg agggaagtag cagcatattt
    99421 ttcataaagt ggctctcttt cttcattttt actttggttg ctcctatttg aaaattcgag
    99481 cacactcaaa tacaataaaa agaaatggca agggagagga ttagatgaaa aaagaagaaa
    99541 aaatcgcgat tttacaagag atcatccgaa ttaaatcagt gaatggaaac gaaggtgaag
    99601 tagcagccta tttgaataag cttttggcaa agcatgacat aacaggagag attgtttctt
    99661 acagagatgg tagagataat ttgattgctc gttaccaaaa gggccaatca ggaaaagtgt
    99721 taggcctatc tgggcatatg gatgtcgtgg cggctggtga tgaatcgtct tggacctatg
    99781 caccctttgc agcagaaatc catgggaacc gtttgtacgg acgtggtgct acagatatga
    99841 agagtggctt ggcagcaatg gtgattgcca tgatcgaact gaaagagtca ggaaaaccat
    99901 ttaacggaac agtcaaatta ttagccactg tcggagaaga agtgggtgaa ctaggtgggg
    99961 aacagctcac gaaagcaggc tatgtagatg acttagatgc cttgatcatc ggagagccaa
   100021 ccaattacag tttgatgtat acgcacatgg gatcaatcaa ctatacagtc acctcgcacg
   100081 gaaaagaagc gcatagctct atgccagacc aaggttataa tgcaatcaat cacctaaatg
   100141 aattcattac gaaagcaaat gctgaaatga atcacctcgc tgaaacaatc gagaatcctg
   100201 tcctaggaaa aacaatccat aatgttacat tgatcagtgg cgggaatcaa gtcaacagca
   100261 tcccaagcca tgcgcagctc caagggaaca ttcgatcgat tccagaatac ccaaatgata
   100321 aaatcattgc attgctgcaa tcaatcgtca atgaattgaa tcaagagaca gactatcatt
   100381 tggaattgat gatcgattac aacaaaatac ctgtcaaagc tgatccagat tctcctttga
   100441 ttcattgcat tcagcaacaa ttcagccagc cattaccttt agttggagcg gcagcaacaa
   100501 cggatgcagc cgaatttacg aaagctaacc attccttcga ctttgtcgtc tttgggcctg
   100561 gtgtcgttac tttgcctcat caagtagatg agtatgttga aatcgataat tatttggata
   100621 tgatcgaaaa atatcaagga attattttat cctacttagc gtaagatttt tttaataaat
   100681 gaaaaaacag agccagatta gcatta
//
LOCUS       DAZUMT010000002        56027 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000002 DAZUMT010000000
VERSION     DAZUMT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.002"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..561
                     /locus_tag="ACG8FM_000103"
     CDS             43..561
                     /locus_tag="ACG8FM_000103"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5092801.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            689..1372
                     /locus_tag="ACG8FM_000104"
     CDS             689..1372
                     /locus_tag="ACG8FM_000104"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS5092802.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1681..1754
                     /locus_tag="ACG8FM_000105"
     tRNA            1681..1754
                     /locus_tag="ACG8FM_000105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1715..1717,aa:Arg,seq:tct)
     gene            1913..2272
                     /locus_tag="ACG8FM_000106"
     CDS             1913..2272
                     /locus_tag="ACG8FM_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS5092803.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2395..4026
                     /locus_tag="ACG8FM_000107"
     CDS             2395..4026
                     /locus_tag="ACG8FM_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS5092804.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4088..4729)
                     /locus_tag="ACG8FM_000108"
     CDS             complement(4088..4729)
                     /locus_tag="ACG8FM_000108"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5092805.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4915..4991
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5011..5319
                     /gene="rplU"
                     /locus_tag="ACG8FM_000109"
     CDS             5011..5319
                     /gene="rplU"
                     /locus_tag="ACG8FM_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS5092806.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5346..5690
                     /locus_tag="ACG8FM_000110"
     CDS             5346..5690
                     /locus_tag="ACG8FM_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS5092807.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5703..5996
                     /gene="rpmA"
                     /locus_tag="ACG8FM_000111"
     CDS             5703..5996
                     /gene="rpmA"
                     /locus_tag="ACG8FM_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS5092808.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6430..9237
                     /locus_tag="ACG8FM_000112"
     CDS             6430..9237
                     /locus_tag="ACG8FM_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5092809.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9428..9919
                     /locus_tag="ACG8FM_000113"
     CDS             9428..9919
                     /locus_tag="ACG8FM_000113"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5092810.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10022..11011
                     /locus_tag="ACG8FM_000114"
     CDS             10022..11011
                     /locus_tag="ACG8FM_000114"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5092811.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11044..11847
                     /locus_tag="ACG8FM_000115"
     CDS             11044..11847
                     /locus_tag="ACG8FM_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS5092812.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11868..12782
                     /locus_tag="ACG8FM_000116"
     CDS             11868..12782
                     /locus_tag="ACG8FM_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5092813.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12865..13239
                     /locus_tag="ACG8FM_000117"
     CDS             12865..13239
                     /locus_tag="ACG8FM_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS5092814.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13378..13623
                     /locus_tag="ACG8FM_000118"
     CDS             13378..13623
                     /locus_tag="ACG8FM_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5092815.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13795..14859
                     /locus_tag="ACG8FM_000119"
     CDS             13795..14859
                     /locus_tag="ACG8FM_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS5092816.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15105..15530
                     /locus_tag="ACG8FM_000120"
     CDS             15105..15530
                     /locus_tag="ACG8FM_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5092817.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15523..15972
                     /gene="nusB"
                     /locus_tag="ACG8FM_000121"
     CDS             15523..15972
                     /gene="nusB"
                     /locus_tag="ACG8FM_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS5092818.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16069..16923
                     /locus_tag="ACG8FM_000122"
     CDS             16069..16923
                     /locus_tag="ACG8FM_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS5092819.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16924..18261
                     /gene="xseA"
                     /locus_tag="ACG8FM_000123"
     CDS             16924..18261
                     /gene="xseA"
                     /locus_tag="ACG8FM_000123"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS5092820.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18266..18490
                     /locus_tag="ACG8FM_000124"
     CDS             18266..18490
                     /locus_tag="ACG8FM_000124"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS5092821.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18492..19382
                     /locus_tag="ACG8FM_000125"
     CDS             18492..19382
                     /locus_tag="ACG8FM_000125"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5092822.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19391..20209
                     /locus_tag="ACG8FM_000126"
     CDS             19391..20209
                     /locus_tag="ACG8FM_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS5092823.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20316..20765
                     /locus_tag="ACG8FM_000127"
     CDS             20316..20765
                     /locus_tag="ACG8FM_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5092824.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20783..22465
                     /gene="recN"
                     /locus_tag="ACG8FM_000128"
     CDS             20783..22465
                     /gene="recN"
                     /locus_tag="ACG8FM_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS5092825.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22520..22636)
                     /locus_tag="ACG8FM_000129"
     CDS             complement(22520..22636)
                     /locus_tag="ACG8FM_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS5092826.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22709..23665)
                     /locus_tag="ACG8FM_000130"
     CDS             complement(22709..23665)
                     /locus_tag="ACG8FM_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5092827.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23836..24204
                     /locus_tag="ACG8FM_000131"
     CDS             23836..24204
                     /locus_tag="ACG8FM_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS5092828.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24374..24805
                     /gene="mraZ"
                     /locus_tag="ACG8FM_000132"
     CDS             24374..24805
                     /gene="mraZ"
                     /locus_tag="ACG8FM_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS5092829.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24826..25785
                     /gene="rsmH"
                     /locus_tag="ACG8FM_000133"
     CDS             24826..25785
                     /gene="rsmH"
                     /locus_tag="ACG8FM_000133"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS5092830.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25790..26191
                     /gene="ftsL"
                     /locus_tag="ACG8FM_000134"
     CDS             25790..26191
                     /gene="ftsL"
                     /locus_tag="ACG8FM_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS5092831.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26192..28384
                     /locus_tag="ACG8FM_000135"
     CDS             26192..28384
                     /locus_tag="ACG8FM_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5092832.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28410..29372
                     /gene="mraY"
                     /locus_tag="ACG8FM_000136"
     CDS             28410..29372
                     /gene="mraY"
                     /locus_tag="ACG8FM_000136"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS5092833.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29386..30747
                     /gene="murD"
                     /locus_tag="ACG8FM_000137"
     CDS             29386..30747
                     /gene="murD"
                     /locus_tag="ACG8FM_000137"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS5092834.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30773..31861
                     /gene="murG"
                     /locus_tag="ACG8FM_000138"
     CDS             30773..31861
                     /gene="murG"
                     /locus_tag="ACG8FM_000138"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS5092835.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31876..33096
                     /locus_tag="ACG8FM_000139"
     CDS             31876..33096
                     /locus_tag="ACG8FM_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS5092836.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33252..34577
                     /gene="ftsA"
                     /locus_tag="ACG8FM_000140"
     CDS             33252..34577
                     /gene="ftsA"
                     /locus_tag="ACG8FM_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS5092837.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34600..35841
                     /gene="ftsZ"
                     /locus_tag="ACG8FM_000141"
     CDS             34600..35841
                     /gene="ftsZ"
                     /locus_tag="ACG8FM_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS5092838.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35858..36535
                     /locus_tag="ACG8FM_000142"
     CDS             35858..36535
                     /locus_tag="ACG8FM_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS5092839.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36549..37148
                     /locus_tag="ACG8FM_000143"
     CDS             36549..37148
                     /locus_tag="ACG8FM_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS5092840.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37229..37450
                     /locus_tag="ACG8FM_000144"
     CDS             37229..37450
                     /locus_tag="ACG8FM_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS5092841.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37499..38281
                     /locus_tag="ACG8FM_000145"
     CDS             37499..38281
                     /locus_tag="ACG8FM_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS5092842.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38302..39009
                     /locus_tag="ACG8FM_000146"
     CDS             38302..39009
                     /locus_tag="ACG8FM_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS5092843.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39271..42069
                     /gene="ileS"
                     /locus_tag="ACG8FM_000147"
     CDS             39271..42069
                     /gene="ileS"
                     /locus_tag="ACG8FM_000147"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS5092844.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42118..43644)
                     /gene="zwf"
                     /locus_tag="ACG8FM_000148"
     CDS             complement(42118..43644)
                     /gene="zwf"
                     /locus_tag="ACG8FM_000148"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS5092845.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43659..44306)
                     /locus_tag="ACG8FM_000149"
     CDS             complement(43659..44306)
                     /locus_tag="ACG8FM_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5092846.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44490..44819)
                     /locus_tag="ACG8FM_000150"
     CDS             complement(44490..44819)
                     /locus_tag="ACG8FM_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS5092847.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44996..45724
                     /gene="sfsA"
                     /locus_tag="ACG8FM_000151"
     CDS             44996..45724
                     /gene="sfsA"
                     /locus_tag="ACG8FM_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS5092848.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45740..46753
                     /locus_tag="ACG8FM_000152"
     CDS             45740..46753
                     /locus_tag="ACG8FM_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5092849.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46753..48030
                     /locus_tag="ACG8FM_000153"
     CDS             46753..48030
                     /locus_tag="ACG8FM_000153"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5092850.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48093..50795
                     /locus_tag="ACG8FM_000154"
     CDS             48093..50795
                     /locus_tag="ACG8FM_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5092851.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50947..51264
                     /locus_tag="ACG8FM_000155"
     CDS             50947..51264
                     /locus_tag="ACG8FM_000155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5092852.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51294..51614
                     /locus_tag="ACG8FM_000156"
     CDS             51294..51614
                     /locus_tag="ACG8FM_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5092853.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51722..53182
                     /locus_tag="ACG8FM_000157"
     CDS             51722..53182
                     /locus_tag="ACG8FM_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5092854.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53250..53471
                     /locus_tag="ACG8FM_000158"
     CDS             53250..53471
                     /locus_tag="ACG8FM_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092855.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53502..53684
                     /locus_tag="ACG8FM_000159"
     CDS             53502..53684
                     /locus_tag="ACG8FM_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS5092856.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53684..54097
                     /locus_tag="ACG8FM_000160"
     CDS             53684..54097
                     /locus_tag="ACG8FM_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS5092857.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54220..55401
                     /locus_tag="ACG8FM_000161"
     CDS             54220..55401
                     /locus_tag="ACG8FM_000161"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5092858.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55472..55636)
                     /locus_tag="ACG8FM_000162"
     CDS             complement(55472..55636)
                     /locus_tag="ACG8FM_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092859.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55790..55863
                     /locus_tag="ACG8FM_000163"
     tRNA            55790..55863
                     /locus_tag="ACG8FM_000163"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55824..55826,aa:Arg,seq:cct)
     gene            complement(55932..>56027)
                     /locus_tag="ACG8FM_000164"
     CDS             complement(55932..>56027)
                     /locus_tag="ACG8FM_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS5092860.1"
                     /translation="MSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        18516 a         9463 c        12080 g        15968 t
ORIGIN      
        1 atatgattaa gaaggattga ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa
       61 ataactttta taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc
      121 ttggttcccc gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca
      181 tattatgcac aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc
      241 ggagatatta ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata
      301 gccgttgaaa acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag
      361 cctggagtag taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt
      421 ggactagtcc tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct
      481 ttttgcctaa caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga
      541 cgaacacaga aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga
      601 ctcttctgaa catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt
      661 cgtataatgt gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt
      721 caaagaagaa ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg
      781 caaactggaa gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt
      841 atttttgaaa tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc
      901 tatcggtttt tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac
      961 aattgatcgt ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact
     1021 gacagataaa gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg
     1081 tccgtatgac tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga
     1141 atgcttacgt ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg
     1201 aacgatcttg ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt
     1261 aaatgaattt cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt
     1321 aggtgatggt gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat
     1381 tatggcagcc tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt
     1441 ttctatattt gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga
     1501 agtcaatgta gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg
     1561 ttttaaaaga ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac
     1621 tatttcgtat tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt
     1681 gtcatagtag ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa
     1741 tccctcctgt gacgctaaaa aaccgttaga aactgaggtt tctaacggtt tttctttgtt
     1801 ttttgtgcca atattgtttg ttttcaaagg caaataaaat aaaaaaacat ccttatcgag
     1861 tacaattata aggagcaaaa aaataaaact catcagaaaa gaggaagaag atatgacgat
     1921 actatctaga attttagtga ctctagttgc aattgaattt ttttatatca tgtatatcga
     1981 aacggtgaga actgattctg acacgacaag tagggttttt aaaatgagta aagaagagtt
     2041 aagcagaaaa tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg gactattagg
     2101 agtgggacta ctttatggag cttatctttc ttccgcatct aaagaaatca caagtatgct
     2161 attaatatct attttttttg ttgcattata tggcagtttg actagcgatc gttggatttt
     2221 actcaaacaa ggtgggctag ctctgttggc attgctttct attttcttat aaatatagaa
     2281 aaaataagaa tttatctaac aggaaacttt gtgaaaaata ttactttatc tcatgaaata
     2341 tgatattcta atgaatggta ttttagaaaa tttcatgaaa aaggagttag ataggtgttc
     2401 aaaaattatg tttcctcact aaaaaaagaa ttttcaggtt atgatctttc aagatttcag
     2461 aaagatcttt tggctgggat cacagtagct gctgtagcat tgcctttagc cttggctttt
     2521 ggggtatcaa gcggagctga tgcggccgca ggtttgatta cggctatcat tgccggtatc
     2581 gttattggcg gtttatcagg cggtttttat caaatatcag gtccgacagg tgcgatggca
     2641 gctatcttga tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct tgctactttt
     2701 ttagcgggtc tatttttact tgcagcagga cttttccgat taggaacact gacttctttt
     2761 atcccagcac ccgtaattac ggggtttact tcaggtattg ctatcattat tgcattaggt
     2821 cagatcgata atttatttgg tgttcactcg gaaggtacaa acgttattga aaaactaagt
     2881 agttatcaaa cgataggatt tccgataaac acagcctctt tgatcatggg agggctagtc
     2941 attttaggga tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg
     3001 gctatcattt tggcaacagc agttatgcta ttgttccatt taccagtagc aactgtgggt
     3061 aagattccac aaacattgat cagtagtaat cgtttgaata tgggagattt tagcttttct
     3121 gccttgcaac aagtggcagt tccagctatt agcattgctt tattaggaat gattgaaagc
     3181 ttgctatgtg gtgcttcggc tggccgaatg gcaaataagc ccttagatag caatcaagaa
     3241 ttaatggcgc aaggaattgg aaatctgttg cttcctttct ttggcgggat tcctgctact
     3301 gcggccatcg cccgtacaag cgtggccatc aaatctggtg cacaaactag attagctggt
     3361 atgatacatg cagttgtctt actgctctcc atgcttgttt ttgcgccagt gatgtcacag
     3421 attccaatgc cagcacttgc aggtgttttg attgtgacgg cttggcgtat gaatgaatgg
     3481 gaaacgatca aggagctatt tactaataaa tattggtcag cagttctatt gtttatcctt
     3541 acgatgattt gtacagttat ctttgattta agtattgcta tcgtgatcgg ggtgatcggc
     3601 ggctgtgtat tttttgttgt caaaagcgct gctatcacga tttcattaga aacgatcgat
     3661 tggcaaagaa tggggctgcc agaaacggaa aagctgaata actggacagt agtgtatatt
     3721 agtggtccac tgttttttat gtcttcagaa aaactcaaaa ataaattgga agaactaaat
     3781 catcacgatg gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt gacggcacgt
     3841 gcagtttttg atgaatttca agaaaaagca gagcaaagag gacaaacgat tatctatact
     3901 tccttgcagc cagaagtaga aaaacaactt tctcacttgt gggaacatac attcacgcat
     3961 caacatcaaa cagttgctca tgcgttgaca gctttacatg cgaaatatac tgcaattagc
     4021 cattaaaaca atgaaaaaga acagacaaga ctgtaaactg aagattttat ggtcttgtct
     4081 gttcttttta atattgctgt aaaagtgaag ctagtacttg ccagtcttga tggataacag
     4141 gtacaaggga gcgattgtag aaaatagctg ctggatgaaa aagtggaaga attcgatagt
     4201 ttttttctga ccattcgtag gcatcctttt gttctgttaa ttgtaagaca ggccctgtat
     4261 agagttgacc atgatttttg gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa
     4321 tgtttccaag tgtcgcaatt aattttggct ggaccttcat cagttcgtaa tccaatattg
     4381 gtgcatgcgc aaatacttcg gaacgagtag gtgtccgatt tggataaaca gtttctgact
     4441 gttgtgtccg tttattgatc cgatgagtca cgcgatacgg gcggctcctt acagcactgg
     4501 tgatatacac ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc aattcttttc
     4561 cagcttgacc gctaaaagga acatgcgtat caatctctgt tttccctggg gcttctccca
     4621 cgagcatcag tttaggatga agaggtcctt gcccagcgat aaatccttct acaggacggc
     4681 cgttcattct taaagaagct tcttgtatca gttcttttgg gtattccatt tatgctcctc
     4741 ctttttcttt tattataaga ataaaagaaa cacaatgcga ctattttatt tggtagaact
     4801 gtgaggatag aaaagcagaa gtattgcttg aatttttgtt ttactttttg attttaaatt
     4861 tagttgacta acggttgctg ttctggtaag ctagtactgt tgtaattgtg gactccacag
     4921 ctacaaccgc tcagaacaag taataagcac agcatatgct gtcacttcgg atggcgagtc
     4981 taagtatatc agaaggaggt gctttatagc atgtacgcaa ttattaaaac tggtggtaaa
     5041 caagtaaaag ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt tgaagcagga
     5101 gaaaaagttg tttttgacga agttatctta gtaggcggag aatctacaaa agtaggcgct
     5161 ccaactgttg caggtgcaac tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa
     5221 gtcgttactt tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa
     5281 ccatatacaa aagtggttat cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc
     5341 tatatatgat caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt tcattcactt
     5401 tgaccgggca tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg
     5461 cgttagcgat cagcactgtc aacgggattg cctcattagc cggttttgag ccgattgttg
     5521 aaatgaatga agaagaaggc ggttatcttt ataccgaagt cacttcagga atgacacaag
     5581 aacaaaacaa tattgctgag attctattag aaaatctttt attaggatta caatcgatag
     5641 aagctgaaaa ctcagaatat atccaaatta aaacaattaa tgataaatag gaggtgcaga
     5701 ccatgttgtt atctatgaat ctacaattat ttgcccacaa aaaaggtggc ggttctacat
     5761 ctaacggccg tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag
     5821 ttacaggtgg atcaattctt taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg
     5881 tgggtatcgg tggagacgac actttatttg caaaagttga tggcgtagta cgtttcgaac
     5941 gtaaaggccg taacaaaaaa caagtgtctg tttatccagt agctcaagaa gcttaattga
     6001 atcttatgat taaagaggtt gtgacaagac ttttgtcaca gccttttttg tttataaaaa
     6061 taaaagttgt agtggaggct acgaagttta ctttgtcttt ttacacttaa tatttttttg
     6121 ccaatttgga atcaggatgt tttgataact atcgttttca attgatggaa gctatattgt
     6181 ttgctatcag tgaaataatt atatggtaaa cagtgttctt tttcttgttt ccattataaa
     6241 aatcgtatcg ggatttttcg ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc
     6301 aacaaaacaa aatcaggtaa atagttgacg aaacagaaat aaatacatag tattatgttt
     6361 tgaagcctaa gtacaatagt ctatagaaaa ggagcgatac cattggcaaa acgcattgac
     6421 cgaatttata tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga atatgatcaa
     6481 ggagtaacaa cacaggaaat tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa
     6541 gatctgaatc agctagtgcg cgaaggaaaa ctgataaaga cagacggacg accagtacgt
     6601 tacatatatc aaacactcgt tacgcacgta aagccagtta caaaacatgt agtcagttac
     6661 aaagaaacgc cggtctctga agaaaaaaat acactccaaa tagatactaa ggatatcttt
     6721 gcaaaaatca ttggagctaa cggcagcatg aaaaattcag tggaacaggc aaaagctgcg
     6781 attttgtatc cgccaagagg attaaattgt ttgattacag gacctaccgg atcaggaaag
     6841 acttattttg cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa
     6901 acggaactga ttgttttcaa ttgtgctgat tatgccaata accctgagct tttgatgagt
     6961 catttatttg ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa aatggggatt
     7021 atcgatcaag cggatggcgg tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa
     7081 ggtcaagaaa tgatctttta ctttatggat catggaactt acagtcgtct tggggaaaca
     7141 acgaaatccc atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga cccaggttct
     7201 tctctattag aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa
     7261 aaacgtcctg caaatgagaa agtcgattta gtaaaaatca tgattgccca cgaagcaaac
     7321 cgcacgcagc gaaagatttc tttgacagaa gatgtagcaa aagcattgat cggcagtgtg
     7381 acctatggaa atataggaca attgaagtca aatatccagt tagtatgtgc acgaggattt
     7441 ctaaatcata tgcaatcacc cgaaatctcg atcacaatcg atgatttgac agaagggatt
     7501 cgtagcggat tgattcaatt agcaagcaac cgcagtgcaa tggcagagct ttctaaaata
     7561 ttggagccaa agataactgt agcaccaaat gatacgatca tgaaaatcca atcggactcc
     7621 tatgagttac cttataatct atatgatatc atcggagata aagcagcttt actgaagtcg
     7681 gatggtttgg atcaagaagc aatcaatcat ttcatttcta cagatatcaa catccatctg
     7741 aagtctttct ataaagacca tggtttttct tttaatgcag acagcaaatt agcagaattt
     7801 gtagatccga aaatcataga agtgaccaat cagatttatg taatggtcaa aagatcttta
     7861 ccatatgagt ttcagcaaaa ctttatttat gctatgagct tgcatatcag ttcttttttg
     7921 aaaaaaatca acttaggaga agaacggcat acgaatgata acatccgtga gatggctttt
     7981 gattttccag atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt
     8041 caagtggaga ttccagaaag tgaagactat tacttgaccg ttctactcgt ctcgttacgg
     8101 gcaaatcaag caagcggcag aatcggtgta gtcgttgcag ctcacggaaa cagcacagca
     8161 agcagtatgg tccaagttgt ccaacagttg ttagatgcag agaacgtacg agcagtagac
     8221 atgcctttgg atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa
     8281 gtagatgaag gaagcggggt catactctta gtagatatgg ggtcccttgc gtcgtttaat
     8341 tcgcaaattc aaagagacac tggaattcca gtacgtactg ttgatatggt aacaacttct
     8401 ctagttttgg aaactgtacg aaaggtctca gtattaggga cagatctaga tatgttgcat
     8461 gaatcattga aaaattttcg aggttacgca gaaatatctt ctgtagaatc tgttcaggaa
     8521 gcaaaaccta ttcataagaa aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca
     8581 gcacaaagaa tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga aacagaatta
     8641 gaagttctag ctttgtcgat tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa
     8701 tatcagatca tagctactac tgggattact gatttgaata tccaagcccc atttattcct
     8761 ttagaacgtt ttattgatca aaatatcgaa gtcattttgg atcagttgct gatggaatca
     8821 gaattggagg aaactgaatt catctcccta gatgaagagt cagctaaaaa tacgtgtgtt
     8881 gagtttatct ctgataactt catctttatt aatggtagta agttaatcga tccaatgtgg
     8941 caattttcta cacaaatctc tcagacgacg ggaatcggcg atgaagaata tggattcaaa
     9001 atcaacctag tgatgcacac agcaggaatg atcgaacgta ttattcggaa tgaaccattg
     9061 actgtggaag aaaacgaatt gacaaataca acgaatgatc ctctttatag ccaattggca
     9121 gcctcagttg ttctgttgga agatcagata aaagtaaaag tgccgataga agaaatgtat
     9181 tatctgctac gtctagtcca taatcagtta gataaaaaag aatacactgt gccatgaata
     9241 cacaaaaacg atacactttt atagtgtatc gtttttgtgt attggttaag aatcctttaa
     9301 attcaattag tgtatcgtaa aaaagttggc acgctttttg cttatagtag tgtgtgctag
     9361 gaggtggttg ttcacacaga aatgataggg gtttctgtga agtagtgagg aagaaggagt
     9421 tttgcagatg gatattcgat tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc
     9481 aacagtttgg tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg atgtagtcgc
     9541 tcaagataaa ttgagaaagt ttctactaaa agaagctgca ccgccgggga tcacagcaaa
     9601 tgtaataacc gtgcagaaaa tgattcaaat ctatcatagt ggattattat cacgagcaaa
     9661 agtgatgctt ttattcacca atccccaaga tgttgaagcg gttgtccgct gcggtgtaca
     9721 gttgaagtcg ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa
     9781 ttttgtttca gtaaatcaac aagatattga ttcctttcgt tacttgaaca gtcaagaagt
     9841 ggagttagag attcgaaaag tacctgctga caggaaaatc cttttgatgg aactactagc
     9901 gaaaatcaaa aaggtgtagc aagaatggtt gttggaaaag gtcaaataat tctgttgtaa
     9961 tggttagcaa ttattaatat ttttgaattt gttacaacaa aagtaaatag gaggtataaa
    10021 tatggtaggg attatcctag caagtcatgg acaatttgct gaaggaatct tacaatccgg
    10081 ttcaatgatc tttggcgaac aagaaaacgt caaagccgtg attttgaaac caagtgaagg
    10141 tccagatgac ttgagagcca aattggaaga agcggttgct tcttttgata atcaagacga
    10201 agtgttattc ttagttgatt tatggggcgg aacaccgttc aatcaatcaa acacattatt
    10261 cgaagagcac aaagataaat gggcaatcgt cagcggtttg aatttaccaa tgttgattga
    10321 agcctacgca tcacgctttt caatggaatc tgctcatgaa attgcagcac acatcattga
    10381 aacagcaaaa gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa
    10441 agcagcagtt gaagacgcac aacctaaagg agcacttcca gaaggaactg tggtcggaga
    10501 cggaaaaatc aaatatgtat tagcacgtgt tgactcacgt ctgttacatg gacaagttgc
    10561 gacagcttgg acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc
    10621 aaaggatgat ctgcgtaaga gactaatcga acaagctgct cctccaggtg taaaagcaaa
    10681 tgttattcct attagcaaaa tgatcgaagt agcaaaagac cctcgttttg gaaataccaa
    10741 agcattgctt ttatttgaaa atcctgaaga cgtattgaca gctgttgaag gtggcgtaga
    10801 catcaaagaa ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa
    10861 agtattgtct atgggacaag aagacgttga agcgtttgag aagcttgaac aaaaaggtgt
    10921 gaaattcgat gtacgtaaag tacctaatga ttcacgcgat aacatggatg atattttgaa
    10981 aaaagcaaaa gctgaattag caaaagcgta ataaataata ttaaaattta taggaggctt
    11041 atcatgtcta ttatttcaat aattttagtc gtattagttg cctttctagc tggtatggaa
    11101 ggtattcttg atgaatttca atttcatcaa ccactagtgg catgtacatt gatcggttta
    11161 gttaccggta acctcgaagc tggtatcgta cttggcggta cgcttcagat gatcgcttta
    11221 ggttgggcaa atatcggtgc cgctgtagca ccagatgctg cattagcatc agtagcatca
    11281 gcaattattt tagttttagg tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt
    11341 gccgttcctt tggctgttgc agggttgttc ttaacaatga tcgtacgtac aatcgctgtt
    11401 ccaatcgttc atttgatgga tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg
    11461 tggcacatca ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc agcagcgttg
    11521 ctatttatcc cagcacaaac tgtgcaatca ttccttgaat caatgccagc atggttaaca
    11581 gatggtatgg ctatcggtgg gggaatggtc gtagctgtag gttacgcttt ggtaatcaac
    11641 atgatggcta caaaagaagt atggccattc ttcgttattg gtttcgttgt agctgcaatt
    11701 tctcaattaa cattgattgc tctaggtgct ttaggtgttg cattagctct tatttacttg
    11761 aacctttcta aaatgggtgg ttcatcaaat aatggaggcg gaagcaattc tggtgaccct
    11821 ttaggcgaca ttttgaatga ctattaatct tgaaggagga aaataaaatg gcagaagaaa
    11881 aaatcaaatt atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag
    11941 gttcatggaa ctacgaacgt atgcaaaacg gtggctgggc attttctatg attccagcaa
    12001 tcaaaaaatt atacaaaaca aaagaagaac gttctgcagc attaaaacgt cacttggaat
    12061 tctttaatac acacccatac attgcttctc caattttagg ggttacattg gctctggaag
    12121 aagaacgtgc gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt aaagtaggta
    12181 tgatgggtcc tttagctggt gttggtgacc cagtgttctg gtttacagta cgtccaatgc
    12241 taggtgcact aggtgcttca ttagctatgg gaggaaatat ccttggacca atcatcttct
    12301 tcttaggttg gaacattatt cgttgggcat tcatgtggta tacacaagaa tttggctaca
    12361 aagctggttc taaaattact gatgaccttt ctggcggatt gttacaagac gtaacaaaag
    12421 gtgcttcaat tttagggatg ttcgtattag ctgcccttgt acaaagatgg gtatcaatca
    12481 aattcttacc agttgtatca actgtaaaat tagataaagg tgcttacatc gaatgggata
    12541 aattgccttc aggtggtgaa ggaatcaaga gtgcatttga acaagttaat aatggcttag
    12601 ccctttctcc tgaaaaggta acaacattac aagacaactt agaccaattg attcctggtt
    12661 tagctgcatt aggattaaca ttgttatgta tgtggttgct taagaaaaag gtttctccaa
    12721 tcgttatcat ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat
    12781 aatccataaa aatatagctc gggcgttgtc ccgagctttt tttatatagt atactgttca
    12841 taaacaaata tctggaggga gaaaatggtt caatcaatca atacaaaagt agacttggta
    12901 attgatgcta cttcgtttac gggtctgaca gattacgggc aaatcatgat aggtaataat
    12961 ggttttgagt tttaccatgc gcgagataga cgcaaatata tccaaattcc atgggaagaa
    13021 gtggattatg tactagcatc agtcatgcta aagggaaaat ggattcctcg atatgccatt
    13081 caaacgaaaa aaaatggtac atacacattt tcttccaaag aacctaaaaa agtgcttcgt
    13141 gcgatcagag aatatgtaga tgaagataga atggtacaat cattgagttt ctttgatgta
    13201 gtgaaacgtg gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag
    13261 aggaggtttt ctttattttt ttactttgca gaagaaaata tgaaatagca aaaccaatta
    13321 tttgtcccct ttaaatccca tatgctaaaa ttgattagca aagaaggagg ttatattatg
    13381 agttggttat ggacattgat tgttggagcc gtgatcggag caattgcagg tgcgatcacc
    13441 agtaaaggaa attcgatggg ctgtgtaacg aatatcattg ctggactagt cggttcaagt
    13501 attggacaag cgattttcgg cgaatgggga ccaaaagctg caggtatggc acttattcca
    13561 tctattttag gcgcagtgat tttggtagca gttgtctcat ttttctttgg aaaacgatca
    13621 taagttttta caaaatagta aactaaaagg agttgcagcg agactgtgac tcctttttgg
    13681 ctttatttat ttaaaataaa aagaagtttt ccggaaattt ttatttaact gtcgtgtacg
    13741 aagaaaagtt tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg
    13801 ctacgtgtag aaaaattgcg caaaaaaatg caagaagaaa atttggattc atttttggtt
    13861 accagtccgt acaatcttcg ttacttaacg aactttacag gtacgacagg attagctgtt
    13921 attactttgg aaaaagcatt ttttattact gattttcgtt ataccgagca ggcggcagcg
    13981 caggctcaag ggtttgaaat tattaaaaat gtcggaccta tttttgatga ggtagcaaac
    14041 ttagtacgca aagaagaact gcactcatta gcttttgagg aaacaacagt ctcattttta
    14101 gaatattcgg ttttagaaga aatcatcgat gcggaattag tacctgtttc acagatgatc
    14161 gaagaactca gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat
    14221 attgcggata tggcatatga tcacatcctg aaaatgatcc aaccgggcat gactgagatt
    14281 gaagtcgcaa atcaactaga cttttatatg cgttctcttg gcgcttcagg tgtttctttt
    14341 gatacgatcg ttgctagcgg attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa
    14401 atcatcgaac aaggtgactt gatcacattg gactttggtt gctattacga agggtacgtt
    14461 tccgatatga cacgtacatt cgctattggt gatcctgggg aaaaattaaa agaaatttat
    14521 caaatagtct tagatgctca attagcagtt attgatgctg caaaaccagg aatgactggt
    14581 gtccaattag atgcagttgc tagagattat atttcaaaac atggttacgg ggaagcattt
    14641 ggacattcta caggacatgg gatcggttta gagatccacg aaggaccaaa cgtttcttct
    14701 cgtgcagaaa agcaatttgt accaggaaat gtgatcactg atgaaccagg gatctactta
    14761 ccaggaatcg gtggagtacg tattgaggat gacttgttga tcacagaaga tggaaatcgt
    14821 gtgttgactc gttcaccaaa agaattgatc atcttatagt ttcatcaata gaagaagtaa
    14881 gcgtaaaaat cagtgttcac agactttgtt atagaaaatc actcgattta atgatacact
    14941 atagaagaac ttttaagctg taagagataa taaagttggg tgttttctcc aattcatagc
    15001 agtttgcagc tgatcggcgg cagttttctt accacgtgat cactatgccg catcatagag
    15061 aattcggatg tatctcgtgc ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa
    15121 atttgatttt aaacgcaaac caagatttag gagagatcgt gatcgctcct gaagtcatcg
    15181 aagtaattat cgggattgca gcttctaaag tagaaggcgt atacggaatg cgcggaactt
    15241 ttgccagcaa tgtcactgaa ttattaggac gtgcagctca cggaaaaggt gtttacttag
    15301 caaacgagga agatggcttg aaagtagatc tttactgtta tctagaatat ggtgtttcag
    15361 ttcctaaagt agcaatggaa atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg
    15421 atatcgattt atctgaagtc aatatccatg tagtagctgt tgttcctgaa aaattagaac
    15481 aaccaacaat cgaagaactt tttgaagacg aagaggaaga gaatgaataa agaattatca
    15541 agacacgaga ttcgggaaat ggcattgcag gctttatttc cattagactt caatgcagac
    15601 ctaacaaaag aagatgcgat tttcaacgca atcgaattag accaccgcga catgatcaac
    15661 gaagatgaat cagagtttgt tcctgtttat cttgatacgc tagttggcgg tgtctgtgca
    15721 aaaaaagatg aattggataa agtgattgaa aaacatttaa aaaacaattg gcacatcaat
    15781 cggatctcaa aaatggattt ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg
    15841 acagatgtac cagcggctgt tgctttaaat gaagcaatcg agttggcaaa aacatttagt
    15901 gatgaccgtt cacgtaaatt cgtcaacggt gttttatcca atgtattgaa agaattagaa
    15961 gctggggcgt aagtcctagc ttttttctat gaatcatcat aaacctgtta taaatcggga
    16021 aaattctatg gtagaatgaa aacacaaaaa atatctggga gtggttagat ggcagaattg
    16081 attaacggaa aagaattagc tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa
    16141 ttgaaagata atggtatcca tcccggatta gtcgttttat tggtagggga aaatccagcc
    16201 agccaaatct atgtaagaaa taaagaacgt gtagcaaaag aaatcggaat ccattcattg
    16261 gtagagcgtt atcctgagac gatttctgaa gacgaactac ttgcagaaat tgaaaaatac
    16321 aatcaagatc ctagttttca tggtatattg gtccaattac cattgccaaa gcatatagat
    16381 gaagaaaaga tcctgttagc tatccgccct gaaaaagacg tagacggttt tcatccgatg
    16441 aatttaggca gactccttgc gggtaaaccg gacaagattc cttgtactcc atacggcatc
    16501 atgaaaatgt ttgaagctta ttctatcgat ccggcgggta aacgagcagt agttatcgga
    16561 cgaagcaata tcgtcggtaa accgatggct cagctactct tgatggcaga tgcaactgtt
    16621 actgttgctc attctaaaac cgaaaatctg gctgaactgg cacaagaagc agatatttta
    16681 gtcgtagcaa taggacgtgg acattttgtc acaaaagaat tcgtaaaacc aggtgctgta
    16741 gtgatagatg tagggatgaa tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt
    16801 gatgaagtgg agcctattgc aggctggatc actcctgtgc caaagggtgt agggccaatg
    16861 actatcacga tgctgatgta tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa
    16921 tagatggcac aagaatattt aactgtcact acattaacga agtatttgaa aagaaaattt
    16981 gatgccgatc cttatttaga acgcgtatat ttaacaggcg aaatatcgaa tttccgttta
    17041 cgtgccaatg cccatcaata tttcagctta aaagatgacc atgcaaaaat atctgctatt
    17101 atgttcaaag gagcattcca aaaattacga ttccagccaa aagaaggaat gaaagtcctt
    17161 gtcgttgggc gtatttcatt atatgaagcg ggaggttctt atcaaatcta tgtagagcac
    17221 atggagcctg atggaatagg tgctttatat caagaattgg aagaacgaaa agaaaagctg
    17281 atgaaggaag gcctttttca agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc
    17341 gcggtcctta ccagtccaag cggagcggtt atccgcgata tcatcacgac ggtgaaaaga
    17401 agatatccaa ttgtccaatt ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg
    17461 gacaatgttg tcaggaatat ccagagagta gaagagatgg gcaatttcga tacaatgatc
    17521 atcggtcgtg ggggtggttc gattgaggat ttatggccgt ttaatgaaga gcgggttgct
    17581 cgggcgattt atcaagctaa tacgccggtg atttcatctg taggacatga aacagatacc
    17641 acgatcgctg atttagtagc agatgttcgg gctgccactc caacagcagc tgcagaatta
    17701 gctgttcctg ttttgtcaga agagttgatg cgcattcaag aacggcaagc tagattgcag
    17761 caggcgtttg tacgccagat ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc
    17821 tatgtatttc ggcagcctga aagattgtat gaaggtcaga ccatcaaatt agaccagttg
    17881 aaacagcgat tatatcaagc tacacagcaa atttatcatg cgaaagaaaa acaaacgatc
    17941 gcattaagcc accaattgga acaagtggct ccgctttatc gagttaaagc agctcgccaa
    18001 gaaactgctt atctagaaaa acgcttagaa gagaaaatcc aacaatatat gaaacaacag
    18061 cagcagcgtt ttgctcaagc tgttcaatct cttgatttgt tgagtccatt gaaaatcatg
    18121 ggaagaggct acagctatac gacgttaaat gatcaagtag taaaaagtgt caccgagatt
    18181 catcaagggg atttgctgac gatccattat caagatggaa tcgttaaagg acaagtacaa
    18241 gagatagaca aaggagaata aaaatatgga aaatccaact tttgaagagt cactgcaaga
    18301 attagaaaaa attgtcatgc agttagaaca aggagacgtt ccattagaat cagctctaga
    18361 ttcgtttaaa cgaggaatgg aattaagcaa acattgccaa gatacattag ctaaagcaga
    18421 agagaccttg acgaaaatga tgacagaatc aaatgaagaa gttgattttg acggaaacga
    18481 ggaaagctga gatgagatta actgactttt cagctattca tctacctgct gtagaaaaag
    18541 aaatcttgtc ttttttagat gagcatacaa cggatagagg actctatgat gcgatgagtt
    18601 attcagtgaa agcaggcggg aaaagaatac gtcccttatt acttttaacg gccgttgctt
    18661 cctttgatga gcctattgat gttcccgtct atcaagtagc agctgcttta gaaatggtgc
    18721 atacgtattc cttgatccac gatgatttgc ctgcgatgga caatgatgat cttcgtagag
    18781 gaaaacctac caatcataaa gtattcggcg aagcattagc tatattggcc ggagatggtt
    18841 tgcttacagg agcattccag ctaatcagta tggcacactt aggtaattct cctaagcttc
    18901 tgctgcttca gcaattagct gtttgtgcgg gatcacaagg tatggttgct gggcaagctg
    18961 cagatatcga gggcgaatcg aaaaagcttt ctttagaaga attagcattt attcatgaaa
    19021 gaaagacagg gcatttgatt cgttatgcct tgctggctgg tggcatatta gcgaagcaac
    19081 ctgaagaaat cttattgcta cttcaacgat tagccgaaca tttaggttta gcttttcaga
    19141 taagagatga tctactcgat gtgattggta caacgaaaac attagggaaa acagctggta
    19201 aagatgaacg tatggaaaaa aatacttacc cgcgtttatt aggcctagaa aagacacgtg
    19261 aggcgttgga gattgagctg ttatccgcca acaagatcat agacaaatta gaagaggacg
    19321 ttttttcttt tgatggcggg ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat
    19381 gagggaaaga atggaaaaag aacgcgtaga tgtattagcg gtcaaacaag gattatttga
    19441 aacaagagaa caagccaaac gttcagtgat ggctggtttg atttacaacg aaaaaaacga
    19501 gcggtttgat aaaccgggag aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa
    19561 aaaacttcct tatgtttctc gagggggatt aaaattagaa aaagcactca aagcatttga
    19621 tctatctgtt gaaggaaaaa cattgttaga tattggtgca tccactggcg gatttactga
    19681 tgcagctttg caaaatggcg caaaaatgag ttatgcttta gacgttggct acaatcagct
    19741 agcttggaaa ataagacaag atcctagagt cgtcgtaatg gaaagagtga attttcgtta
    19801 tgcaaaaccc gaagacttta ccttaggaat tccagaagtt gctgttatcg atgtttcttt
    19861 tatttcacta aaactgatgc tgcctccatt acatgctatt ttaaaagaag agggagaagt
    19921 gattgcttta atcaaacccc aattcgaagc aggcagagaa gctgtaggga aaaacggaat
    19981 tgttcgtgac cctcagaccc atcaaaaagt tttagaagac attttgtcat ttgcggcaca
    20041 aggtggatat gatgtgctgg aattaagtta ttctccgatc acggggggag aaggaaatat
    20101 cgagttcttg gctcacttga ggaaagtacc cgaaagcgga acaatcaatt cagctattaa
    20161 tatggcagaa gtggtttcaa atgcacacga acagtttgac cataaataaa ttgtaactgt
    20221 gaaaatcaag tgagaagtat gcggattctt ctttattata ccaaggaaat aagctatcat
    20281 tgattaagaa gaatatgcta aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt
    20341 tgattactcg attgttgagt gagcaagata tccgaaagca ggaagaattt gttgaaatcc
    20401 tgaaaagcaa aggaatatct gttactcaag ctacgatttc cagagatata aaggaactga
    20461 aactgatcaa ggtaccggct tcagatggcg gctaccgtta tagcatacca gctgaaacaa
    20521 acgaagatgt aaatactaaa ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc
    20581 aaatggaaaa atttgttatt ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt
    20641 tgattgataa acgttacaag aaagaattat tttcgattat caatgatgac gataatgttt
    20701 tgatgattac acgaactgaa gaccaggcag ttttattgaa gaaagacttc ctgcattacc
    20761 tttaagttga ggagcgaatg tgatgctaca agaaatcagt attactaact ttgcgattat
    20821 tccggaattg cggctttctt ttcacgaagg aatgaccgcg ctgaccggag agactggtgc
    20881 gggtaaatcc attattatcg atgctttagg tctgttagca ggaggaagag gttcaagcga
    20941 ttatatacgt caaggagcag aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca
    21001 agaaggattc tctgaactga tggttgaatt agggatcgaa acagatgagg acaatctgat
    21061 tgttcgacgg gatatgtcct taactggaaa aaatgtttgt cgagtgaatg gtcatattat
    21121 cacattagct aatctgagaa gaatcggcag ttatttagta gatatccaag gacaaaacga
    21181 acatcaggaa ctgctccagc ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt
    21241 agctttccaa cagaagaaaa agagttatca gcaagaatat atctcttatc gtgagctgga
    21301 aaaaagggta cgaaaagtgc agcaaaatga aaaatcctat gttcagagaa tagatatgct
    21361 ccatttccag caagaagaaa tagctgctgc tgatttgcaa gtcggagaag aagagaaatt
    21421 aaaagaagaa agagaaaaac ttagcaatta tcaaaaaatt gctgatggat tagctgcagg
    21481 ttacggagca ctgacgaata atgaacaaaa cagtgtagat ggtgttggct tggccgtttc
    21541 agaactgcaa gggatcgccc atctagatgt ggagtatgaa gcaatttatg aaaatatcca
    21601 aagtgcttac tatttgctgc aagatgcaat cggcgatatg agtcgtcaga ttgatttatt
    21661 ggagttagat gagagtcgtt tagaagaagt tacgcaacgt ttagaattaa tccgacagtt
    21721 gaaacgtaaa tacggcgaat ccattgagtc aatattggct tactatgacg aaatcaccga
    21781 agagctagct tcttctgatt tttcagaagg tcaattggac aaaatgaaag aggaactaga
    21841 gcaaaaagag cttctacttc agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat
    21901 cgcaaaagaa ttggaaaaat ccattctgca tgaattgaaa agtctgtata tggaaaacac
    21961 tgagtttgag gtacgtttct taaaagaaga aaatagacaa ttgaatcatg atggatttga
    22021 ccaaatcgaa ttttacatta cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt
    22081 cgcttctggt ggcgaactgt ctcggatgtt gcttgctttg aaaactattt tttcttctga
    22141 gcaaggtgtg acaagtatta tttttgatga agtggataca ggtgtgagcg gaagagttgc
    22201 acaggccatc gcagataaaa tttctcaaat atcgaaatac tctcaagttt tgtgcatcac
    22261 tcatctgcct caagttgctg cagtcgccga ctatcaatat tatattgtga aagcagtgat
    22321 tgatggacgt acgcaaactt ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat
    22381 tgcacgtatg ctggctggaa gtgagattac aaaactcaca ctcgaacatg caaaagaact
    22441 attgaaactc gcaaaaagga cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc
    22501 tgatagctat attttatttt tacatgacac tcattacagc agtcaataca acagatccaa
    22561 tcgttaaaat cagcattgtc caaatgacaa attttgttac tttagcaaaa gtacttgttt
    22621 gttttttttc acgcattgtt ttcaacctcg tcattaatta gttgattcaa gtgtacaatc
    22681 ttttttccat ttccacaaga gggctttttt ataaaaactt cccttttttt aaataatagg
    22741 tagttacgat acaaataaat aatgttaata gaactagcca tataaaggca tcatcccttt
    22801 cagcaatagg taacttaatg ttcattccat agatactgcc gacgattgtc ggtatagtta
    22861 aaataatcgt taaagaagtt aaaattttca taatattatt cagattattc gatacgattg
    22921 ctgaaaaagt ctgattcatt tgttctacta gttttaattg aatgcgagaa gtagtcattg
    22981 cctgctctgt ttccaccaat acatcacgta aatgattttt caaagcatga ttttcttgga
    23041 aaagtttatt ttgcgctagt tctttcaatg catctaaatt agcagaagtc gcttctctga
    23101 aataaacgag gcttttttgg atatccatga tttgataaag ttgtttgttt tcagtggata
    23161 cttgaagttt ggcttccaaa gtatcgcatt gagattgaag cgtagctaaa taactgttgt
    23221 aagagagtgt gacctgccaa atgaattgaa agaagatatt gagattaggc gaaatatcac
    23281 tttttggaaa ggtctggcta aagactttct ttaaaaacag tggttcatga ttcgtaattg
    23341 tgataagctt ctttttgggc gtcaaaataa tagagagcgg gtatgtatcc acttgaaaat
    23401 aaccgctagg acttgtttta gcaaaaggat attgtaacaa taataacgct ggctttgtca
    23461 actttgattg attgaattct tctgtacggg aattttctgc atcgtccaat atggaagtga
    23521 tgtaatctga cggaatttca aattgatgtg tcagctgatg aatctcttct tgattaggac
    23581 gttctacaca ataccagatc gtttggtcag tttcctttcc ggtattacgc agtttctcat
    23641 tatctaatgt aaaatatttg atcatgattt tcctcctgtt gcctgtttct tttattataa
    23701 cggataggga attggaatac atggaatgtg tttgtaagat atagaatata actgcataaa
    23761 tttaaataaa atgaaacagt tgtgtaaagt atatacagta tacttgaagc aacgataaaa
    23821 aaggagttta cgttcatggg cacattcaca ccacttatct tgttttggta tatttacccg
    23881 gtaatcgttt tgtttggttg ccaatttctt gtaaaattat tttcgttaaa tcgtcgtttt
    23941 aaaataaaag cccctgattt ggcgattcct tttttgtggg gcggtatgca tgcattgtct
    24001 agaaatacag ggacaatttc tatcttaccg tttttcttta tttctgtttt gctaatggga
    24061 atttgtattg ctgttttcca agcatattat tatgaagaaa ttatttatcc gcgttatttc
    24121 aagatgactt ggcgtttggt ttttctgttg acaatcgtct tgtacgctgt cttgatcatt
    24181 gtgaacatac tttcttacct atgaaaaaaa tagaaaagct gtaaaaagtc ttttaaaagg
    24241 ctttttgcag cttgattttt aaaaaaatgt ttatgaataa aaaaagttgt tcccggattt
    24301 cccagaaaag gtggtagaaa gtgggggatt gtggtagact aattttagtt agtggaggaa
    24361 tggggggctt tcaatgttga tgggtgaatt ccaacataat attgatgcaa aaggacgttt
    24421 aatcgtgccc tcaaaacttc gtgaagaact tggcgaaaag tttgttctga cacgcggatt
    24481 ggatggctgt cttttcggct atccgatgag cgaatgggaa aatctggaag ctaaactgaa
    24541 cgaaatgccg ttggctaaaa aagacgcacg tacttttgtg cgattcttct attcggctgc
    24601 cactgaatgc gaacttgata aacaaggacg aatcaatata cccagcacgt taagaaatta
    24661 tgcagctttg acaaaaggtt gcgtagtcat cggtgtatct aaccgaattg aaatttggga
    24721 tgagacacga tggcaagatt tttctgcagc agcagaagag aactttgacg aaattgctga
    24781 aagtatgatt gatttcggac tataagaata ggaagaggct gactaatgac tgaaacattt
    24841 caacactata cagtcatgtt gaaagaaacg gtagatggat tgaacatcaa accggatggc
    24901 gtttatgtag actgtacact cggcggtgcc ggacatagtg aatatttatt aagccaacta
    24961 agttctaaag ggcatctgta tgcgtttgac caagatcaaa aagcaatcga tcacgccaag
    25021 ctgcgtttag cttcttatgt cgaacaaggt atggtcacat ttatcaaagc gaacttcaga
    25081 gaattagaag aacgcttgag tgagcatgta gataaggtag acggcatttt gtatgatcta
    25141 ggggtttcct ctcctcagct agatgaagca gaacgtggat ttagttatca tcaagatgca
    25201 ccgttggata tgcgcatgga tcagtcagct cctttatccg cttatcacgt aattaatgaa
    25261 tattcctacc atgaattagt aaagattttc ttccgctatg gcgaggagaa attttccaaa
    25321 caaattgctc gtgaaatcga acgagtacga aaaacaaaac caatccaaac aactggtgag
    25381 cttgtcgaaa tcattaaaca agtcatccca gctcccgcaa gaagaaaagg cgggcatcct
    25441 gcaaaaagaa tttttcaagc tgtgcggatt gcagtaaatg acgaactcgg tgccgaagag
    25501 gcctctttag aacaggcaat ccggttattg aaagtcaatg gaagaatcag cgtaatcaca
    25561 ttccattcat tggaagacag aattgtcaaa agtatgttta aagaatatag tacggtacaa
    25621 gatctaccac cgggacttcc tgtcatacct gacgaattcc aaccagaact gaaagtcatt
    25681 actcgtaaac caatccttcc ttcagaggaa gagctaacag aaaacaatcg atcacgcagt
    25741 gcaaagttac gtattgcaga aaaaataaga atgaaagagg agtagaatga tggcagaatt
    25801 aaagaaaatg caagattacg aatatgattt acctgaattg gtggatcagc cggaacaaca
    25861 aatgcctgat tcttcttcga atcaagaaat cattcttcca caatcaccgg caaaacgatt
    25921 aaagcagatt tctgctttgg aaaaaattgc tgtggcaagc gtggcgattg ctattgttgc
    25981 tttatgtgtt ttaacagtta tgctgcgcac aaatatcagc ggagctgaaa aagaaatcac
    26041 gtcaatacaa acagaaatca atgataaaaa gcaagaaaaa acgagtttag aacaagaaaa
    26101 aaacgaactt tctcgtactg aacggattaa acaaatcgca gaagaaaaag gattgtctat
    26161 taatgacgac aatttaagga aagtgaagta aatgagtctt aaaaataaaa ttcggcggtt
    26221 tatggaaaag aaaaatctaa atccgatgaa caatcgcaaa aaagtaggta tcattttatt
    26281 tgctacgagt attggattgt tctttttatt tgccgtccgt ttctcgtata tcgttattgg
    26341 aggtcatgtt gctggtacct cattagctga aaaaacaaaa cagttgtacc aaggaagtga
    26401 agtagtaaaa gcaaaaagag gaacgattta tgatcgtaac ggtgtggcgc tcgcagaaga
    26461 tgcctcctct tattcaatta aagcgatact ctcaaaaaca tatacctctg gggataaaaa
    26521 actgtatgtt gaagaaaaga attttgataa aatagcagaa atcctgcata agaatctttc
    26581 tatagacaaa aaagatgcac tgaatatttt agaggacggc gcaaaaaaag agctgtatca
    26641 agttgaattt ggtagctatg ggaaaaatat ctcccaagaa accaagcaaa acatcgaagc
    26701 ggacatgaaa aaagaaggtg tcgctggtct ttattttgtc gaccatcagg ctcggatgta
    26761 tccaaatggc gtcttttctt ctcattttat tggatacgca gttccagaca aagatgagaa
    26821 tggcttagtt ggtaaactag gtttggagtc tgcttacaat gatatcttga gcggtaaaga
    26881 tggtaagatt atttatcaaa aagataattt tcaaaatcct ctgccaggta cagtggcaga
    26941 agaagaaaaa gctgtcgacg gacaagatat ctacacgact ttagacagca gactacaaag
    27001 ttatttagaa acactgatgg atcaagtaaa tgaagaatat cagccagaag aattgacagc
    27061 agtcctgatg aaagcaaaaa ccggagaaat cttggcaatg ggacaacgac ctactttcaa
    27121 tcctgaaaca atggaaggat tgactggaaa agatgctatc tggcgtaatt tcttggtaca
    27181 agatagttat gaaccaggat caaccatgaa agttttcact actgcagctg ctatcgaaga
    27241 aggagaattc aacgagaatg aaaccttcca atctggaaaa atccaagtag aagatgctac
    27301 gatcaatgac catgactttg gtgaaaaagg tgtgctgacc atgcgtcaag ctctttcatg
    27361 gtcaagtaac gtcgggatgg tcatactgga acagcgatta ggcggacgat ggtataacta
    27421 tttacaaaaa ctgggatttg gacaaagtac tcattctgga ttagatgatg aagtaaacgg
    27481 tgctttacct acattaaata ttgtcgaccg agcaatgagt gcttatggac aagcggtagg
    27541 tgtgacgaat ttccaaatga tgaaagcttt cacttctatt gcgaataacg gtacaatgat
    27601 tcaacctcga tatatcagca aagtggtgga tccccaaact ggagaagagc gaacaactca
    27661 aacagaagtt ctagggcaac ctttttcaaa agaaacaaca gaaaaagtcc gtgaatatat
    27721 gagagatgtt gtcgaaagcg aaaactacgg tagtgcttat ggtgtatata gtgtacctgg
    27781 atacaatgta tcggcaaaaa acggaacagc ccaaatcgct tctgatacag ggggatatca
    27841 aacaggagat acagcctacc tgtattcgat tgtggagatg gttccttctg aagatccaga
    27901 ctatgtcttg tatctaacaa tgaaacaccc taagacatat gatcgaatgg cattggctaa
    27961 aatcgcaaat ccattgatga aacgggcaat ggattttaaa gaaactgaag aagattcaga
    28021 tacagaaaca aaaacagaaa aagtatccgt agccgattat cgtaatcttg aagcggacgt
    28081 tgcagcagct gatgctcaaa aaagtggact gcaacctgta gtgatcggta atggtaaaaa
    28141 agtacaaaaa caatctactg ccaacggtga tcagttgatc tctggagaaa aactgatcct
    28201 atatactggt ggtgataagc tgatgcctga cgtaacagga tggtcaaaag cagatatcat
    28261 gaagctgggg aaaatcctcg gtattgaagt tagttttgat ggagatggtt attgtgtcaa
    28321 acaagaactt gctccatatg aaaaaatcac tgaagacaaa cttagtttta ctttagaaga
    28381 ataagaagag aaataggaga gaaaagagca tggaatggac acaagcattg attcccattg
    28441 tcagcagttg tgcaatgaca attgccgcaa tgccgctatt tattggttat tttcaaatga
    28501 aaaagcaagg tcaggcgatt cgtgaggagg gaccaaagtg gcacaatagc aaagccggaa
    28561 ctcctacaat gggcggctta gtgtttctga tcggctctat tttgacagga atttgggttg
    28621 gagcttggca aaaacagcta actcctactc ttttcatctt gctttttgta ttagcgttat
    28681 acggagtaat cggctttctt gatgatttta taaaaatatt caagaaaaga aacatgggac
    28741 tgaactcgaa gcagaaattg attggacaga ttctaggggg aatcatcttt tatcttgttt
    28801 accgttctga aggttatcca ggtgtattga atttcttcgg actagatcta cctttaggaa
    28861 tcatttatgg tattttcgct ctcttctggc ttgttggatt ttcaaatgca gtaaatctga
    28921 cagatggcat cgatggatta gttgctggct taggaagtat ctcttttgca gcttacggtc
    28981 taattgcttg gcatcagcat cagtatgatg tattaatcat ttgcttaagt gttttaggag
    29041 gtttactggg tttcttcgtc tacaacagaa aacctgccaa gatttttatg ggagatgtcg
    29101 gttcattagc gttgggcgga cttttagcgg caatttctat aatgctaaac caagaatgga
    29161 cattgctttt gattggtttg atctatgtta tggaaacagc aagtgtgatg cttcaagtta
    29221 cttcctttaa gcttacagga aaacgaattt ttaaaatgtc tccaattcat catcactttg
    29281 aaatgtgcgg atggtcggag tggaagattg atacagtttt ttggttgatc agcattgtga
    29341 cttcgttgat cacattatgg atcgtttggt aggagatgac gggaaatgaa aaaaatatca
    29401 acatatgaaa ataagaaagt gcttgtctta ggactcgcaa aaagcggagt aagtgcggcc
    29461 aaattattac atgaattggg cgcacttgtc accgttaatg atggaaaacc ttttgacgaa
    29521 aatccggaag cacaagagct gctttcttta gggataaaag taataacagg tagtcatcca
    29581 atcgaattgt tggatgaaga attttcattg atggtcaaaa atccagggat tccctacagt
    29641 catcctttag tagcaaaagc ccaagaaatg ggtattccgg taattacgga agttgagcta
    29701 gcttacgaag tagcggaatg cccaattatt gggattacag gaacgaacgg aaaaacaacg
    29761 acaacaacga tgacaggact actactgaat gcaggagcag atcaaggaat tgcacgttta
    29821 gcgggtaata ttgggtatcc agcaagtggt gtagcgcaag aagcaaaatc agaagataaa
    29881 attgtgatgg aattgtctag cttccaactc atgggaatca cagatttccg ccctcatata
    29941 gcggtgatta ccaatatcta tgaagcacat attgattatc atggtacaag aaaagaatat
    30001 gtcaaagcta aatggaacct tcaaaaaaac atgacagaaa aagattatct gatccttaac
    30061 tggaatcagt cagaactaca agaattagcg caaagaacaa aagcacgcgt actgcctttc
    30121 tcgacaaaag aagtgttgga agacggtgtg tatgctgacg attactcaat atactataaa
    30181 aaagaaaaaa taatggaaat atctgaactt ggcgtaccag gtaagcataa tgtagaaaat
    30241 gcactagctg ctatttctgt agcaaaattg tatggtatat caaatgaagc tatacgagaa
    30301 acgttgcatt ttttccatgg cgtaccacat cgtacgcaat atgtaggaga aatccaaggc
    30361 agaaaatttt ataatgattc aaaagcaacc aatattttag cgactaaaat ggctctaagt
    30421 ggatttgaaa cgtctaaggt cgtgttattg gctggcggat tagacagagg aaatacattt
    30481 gatgaattaa ttccttcatt aaaaggaatc aaagcaatgg tcgtatttgg ccaaacaaaa
    30541 gaaaagttga tggatgctgg aaaaaaagca ggcatcgaga caatagtaac agccgattct
    30601 gtagaacaag cagttccttt agcattagaa aacagtacag atggtgatgt agttttatta
    30661 tcaccagcga atgccagctg ggatcaatac ccaaattttg aaactagagg aaacagattc
    30721 atggaagctg tgaaccgatt aaaatagaaa aagtagaagc gagcgataaa aaatgaaaat
    30781 tttggttaca ggcggaggaa cagggggaca tatttatcca gctcttgcct ttgtcaatta
    30841 tgtaaagact caagaaccga atgctgaatt catgtatgtg ggagcaaaac gaggtctaga
    30901 aaacaaaatc gtccctgata caggaatgcc tttccatacc ttggagattc aaggatttaa
    30961 acgaaaaatt tccatgcata acatcaagac ggtccgattg tttcttaaga gtatccgtca
    31021 agcaaaaaaa atcctcagtg attttcaacc agatatcgtt atcggtactg gaggatatgt
    31081 ttccggagcg gtagtgtacg ctgcagccaa aatgggtatt ccaacagtta tccatgaaca
    31141 aaacagtgta ccaggtgtta ccaataaatt tttaagccga tatgtggatc ggatcgctct
    31201 ttcatttaaa gatgcggctt ccttttttcc tgaaaacaaa gcagttcttg taggtaatcc
    31261 tcgagcacaa gaagtagcag atactaaaaa atcagatatt ttgaaaacat ttgatttgga
    31321 tccagaaaag aaaacagtct tgatttttgg cggtagtcaa ggagcactta aaatcaatca
    31381 agcagtatca gtttttttaa gtgaatatat tccttcttct taccaagttt tgtatgcttc
    31441 aggagataga tattatgaag agatcctaaa taaaataaat cctgaaatac aaaatattag
    31501 tgtccagcct tatatcaaaa atatggcaga ggtcatggct tccagtgatc tgctggtggg
    31561 gcgtgctggt gcaacttcga tcgcagaatt cacagctctg gggttaccgg cagtgttggt
    31621 tcccagccca tatgttacga atgatcatca gactaaaaat gcaatgagtc ttgttcacgc
    31681 aggagcagca aaaatgattg cagataatga actaacaggt gaatcactaa gtcagacagt
    31741 caacgaaatc atgggtgatg aagaattgca aaagcaaatg tgccgagctt ctaaagaaca
    31801 aggaatccct gatgcttcaa aacgtcttta tgacttagta aaacaaataa tcaaaaaata
    31861 aaggcggtgg tcttagtgag taagaaaaaa gaaccaaaaa aattgtctca tccgtcttct
    31921 gatgaaaaca atttgactcc ttggcaaaag gctaaccgaa aatatctggc agaacaagag
    31981 atgaaaaacc aagagccaaa aaaagaagac ataacattag atacacccct ttcagattct
    32041 gaagaagaaa aaacggttca agaggaacag caagaagaaa gccagtccga gaaaaaaatc
    32101 gaattttttg aagagatcaa gcaaaagaaa aacggtggac catataacgg ttcttttttg
    32161 aaccggctgc ccaatttaaa aagtcaacgg cacaaagtac tatttcgtcg gttagctttg
    32221 atcatcactg tattgggtat accgctgatt tttttgatct attatgtttc tccttatagc
    32281 aaattacaag cagttgcagt atcaggcaat aagacagtca acagccaaga aatcatctct
    32341 gatacaaaat tgtcattagg agaaaatgtt tggggacagt attttcatcg atctacttat
    32401 atcgatcgcc ttaaaaaagc acagccaaga atcgaaacag caaatataca tttcaaaggg
    32461 atgaatgaat tcgatttgga tgtaacagaa tataaagaga ttgctttgat cgcaaaaaac
    32521 aatcaatact acccagtgat tgaaaatgga acagtattag atgaaaaagt agcaaatcct
    32581 acaaaaaatc tccctatctt ggaagagttc aaagactctg caaaaatcaa agaattaaca
    32641 aagcaataca atcagttatc atcagaatta caaaaagcaa tttctgaaat caagtacacg
    32701 ccaagagcaa gcaataaaaa tctgatccaa ttgaatatga atgacggcaa tcaagttatt
    32761 gtaaatatca ataatcttgc gaaccaaatg aaatattatt cgcaagtagc caaagatatg
    32821 gatgaaaaag gtgtgatcga catggaagtt ggtatcttct cgcatcctta cggtagcact
    32881 caagaaacgg aagaatcggg ccaatcggaa gaaacaactg aaagtgaaga aaatgcggat
    32941 caaacaaacg aatcaagtga agaggcagaa acgcaaaaag cacaaacacc tggatcgtct
    33001 agcacaaacg gatcatctga ttcaaatgat tcaagtgaaa caagtcaaat agaagataat
    33061 ccaagtacaa ctccgtcaag tttaaatgaa aattgataaa acaactttaa aaagtaagaa
    33121 tgtactttct aaaaaccagt aatttatggt agaattggaa gcagtgaata tatctattaa
    33181 taagtatatt ttaaaacgga cgctagttgt tcgcttgtat atggaatagt aagtaggagg
    33241 gaatcccatt catggcaaaa acaggaatgt atgtaggcct tgatattgga acgacatctg
    33301 ttaaagttgt cgtggctgaa tatatcgaca gccaaatgaa tattattgga gtaggaaacg
    33361 caaaatcaga agggattaac cgaggcatta tcgttgatat cgataaaacg gtccaagcga
    33421 tacaacgagc agtacgacaa gcagaagaaa aagcaggtat ccagatcaaa ggcgtaagtg
    33481 tcggtttgcc agcaaacatg cttgaagtag aaaattgcca gggaatgatt gcagttaacg
    33541 gagattcaaa ggaaatcacc gatgaagatg tcagaaatgt ggcttcagct gcccttgttc
    33601 gttcaatacc tcctgaacgt caaatcgtgt caatattgcc acaagatttt actgtcgatg
    33661 ggttcgaggg aatcaaagat cctcgtggaa tgatcggcgt aagattagaa atgtacggtc
    33721 tgttgtttac aggacctaaa acgatcatcc ataacatacg taaatgcgtg gaaaatgccg
    33781 gattaatcgt caacgaaatg gtgattactc ctttagcgct gactgaatca attctttctg
    33841 acggagaaaa agattttggt acgatcgtca ttgatatggg tggcggccaa acaacaacgg
    33901 ctgttatgca tgataagcaa ttgaagttta ccaatcttga ccaagaaggt ggcgaatttg
    33961 tcactaaaga tatctctatt gtattaaata cgtcatttaa caatgcagaa gcgttgaaaa
    34021 taaactacgg ggatgcatac ccagaacgga catcaccaga tgaagaattt ccagtagatg
    34081 tgattggtca gtcagaacca gtgaaagtcg atgaacgcta cttgtcagaa attatttcag
    34141 ctcgaatgga acagattttc aacaaagcaa aagatgcatt ggaccaaatc gaagcattag
    34201 agttgccagg tggcgtcgta ttgacaggcg gagctgctag tcttcctggt gttgtggact
    34261 tggcacaaga aatcttcggc gtaaatgtaa aattgtatgt tccaaatcac atggggctga
    34321 gaaacccggt atttacaaat gtaataagta tcgtggatta ttcagcaaac ttgagtgaag
    34381 tgtatcaatt ggcaaaaatt gctgttacgg gtgaaacctc agcagctcgt caaatggtag
    34441 ttgaacaaga aacaacaaac acatatgaga gctatgaagc acctgaagaa acaatctacg
    34501 atgagccgga accaaaagag tccggagaaa atgtaaaaaa taaaatcaag ggtttcttta
    34561 caaatatttt tgattaattc atcctgggag gaataagata tggaattctc aattgataat
    34621 aatatcaatg acggcgcagt aatcaaagtc atcggagtcg gtggtggcgg tggaaatgct
    34681 gtaaaccgaa tgattgaaga aaacgttaaa ggtgttgaat tcattacagc caacacagac
    34741 gtgcaagcat taaaaaattc aaaagctgaa acagttatcc aattaggccc caaatatact
    34801 cgtggtttag gtgctggttc tcagccagaa gttggacaaa aagcggcaga agaaagtgaa
    34861 caatccttac gcgaagcttt agacggtgca gatatgattt tcatcactgc aggaatgggt
    34921 ggaggaaccg gtacaggtgc tgcaccaatc gttgcaggta tcgcgaaaga attgggcgca
    34981 ttgactgttg gtgtcgtgac acgtccattc acttttgaag gaccgaaacg tggccgtttt
    35041 gctgcagaag gtatcgctcg tttgaaagaa aatgtggata cattattaat tatctcaaat
    35101 aaccgtttat tagaagttgt agataaaaag acaccgatgc ttgaagcttt ccgtgaagca
    35161 gataatgttt tacgacaagg tgtacaaggt atctctgatt tgatcacagc gccaggttac
    35221 gttaaccttg actttgctga cgtgaaaaca gtgatggaaa accaaggtac agcattgatg
    35281 gggatcggtg tggctagtgg agaagatcgc gtagtagaag caacgaaaaa agcaatctcc
    35341 tctccattgc ttgaaacatc tattgatgga gctgaacaag tacttcttaa catcacaggc
    35401 ggtttggata tgacattgtt cgaagcacaa gatgcttcag atatcgtagc aaatgcagca
    35461 actggcgatg taaatatcat tctaggtaca tctatcaatg aagaaatggg cgatgaaatc
    35521 cgtgtgacgg tcatcgcaac aggtatcgat gaatcaaaaa aggaacgcaa gtctagccga
    35581 ccagctagac aagcacaaat gcaatcacct gctcaaaaga ctgtattaga tatggatcaa
    35641 gctaaaccaa cttcttcaga agaagaaaac agttttggcg attgggatat cagacgtgaa
    35701 caaaatgttc gtcctagagt tgatgattcc aattttgaca atatcgaaaa aaaagaattt
    35761 gatacattca accgcgaaga aacaaaatct catggcgatg atgaattaaa tacgccaccg
    35821 tttttccgtc gtaaaaaata aaggagagga agagcacatg attgctgaca acttgcagaa
    35881 aatcaagcaa gaaattcagg attcgtgtgc tcttgttcat cgtgatccga aagaagttac
    35941 attagtagct gtaacaaaat ctgttgaatc cgctcaagct gaagaattgg ttagagctgg
    36001 cgttatgaat tgtgcagaaa accgagtaga taaattatta gaaaagaaac aagatttatt
    36061 tgattttcct gaaataaagt ggcatttaat tggtaattta cagcgcagaa aggtaaaatt
    36121 gattgtaaat gaaattgatt actttcatgc gttggacagt ttaagattag cagaagaaat
    36181 ccagaagcgg ttagagaaag aattatcctg ttttattgaa gtaaatgttt ctggtgagga
    36241 atcaaaacat ggaatttcac cagatgagct tttaccgttt gtggaatctt tagctgcctg
    36301 cacaaaaatc aaggttgttg ggttgatgac tatggcgcct aaagaagctt ctgatgagca
    36361 aatccataaa gtgtttggta cgctgaaaga attgcgtgat cttgttcaag caaaaggtta
    36421 tgcccatgca ccatgtagtg aattaagtat ggggatgagt caagactttc aaattgcgat
    36481 tgaagaaggt gcaacattta tccgtgtagg aacagcgcta ttcaaagacg aataaaggag
    36541 gaacaaaaat gccgctttta aacagaggag caattgctaa ttttttcgga ttagctgatg
    36601 aggaagagta cgaataccaa gattatcctg aaacaacaaa aaaggctgta cagccaacta
    36661 aaaaaaatac aacacagaat caatcaactc attctgtatc tgctgcgaat actcatgaaa
    36721 aacaaaaaac ggcgacttca cgtcctgtaa cgcatgaaag tattcagccg gctgaaaaga
    36781 aaaaagtaat tgaactacat caatcaccta caaaaactgt agcgaaagaa agaacagctt
    36841 caagaacctc tacacaacct aaagcaaatc ttgctggaaa aatcactgta atggaaccaa
    36901 gagcttattc tgaagctatg gcgattgcta aacgaatcat tgctggagaa gcaaccttag
    36961 tgaattttca tttagtagaa gaaaaacaag cgagaagaat cgttgatttt ctaacaggaa
    37021 cagtttatgc actagatgga gatatcaaac gtgttggaga tgagattttt ctttgtacac
    37081 cgactgggat ggagattgac agttcaaccg ctcaatcttt tgcagatgcg aatttgtttg
    37141 atctgtaaga ggaggaacta cgatttatac tattgcacta ttacttcatt tacttcaacg
    37201 ggcagcctat ttctatagca ttatcttgat ggtatatgca ttgctctctt ggtttccagg
    37261 cgggtatcaa tcagggctag gacgattatt agctaagatc tgcgaaccgt atatcagttt
    37321 gttcgatcgt ctgcctttga ggattggacc cttggatttc acaattttag gggcaatcct
    37381 gatcttgaac ttagccgtac aaggtttagc acagatcgtt atatggataa ctgtccattt
    37441 agtttattga ttgaagcaac aaaatcttta ttctcagaat agataggagg ggaaaatttt
    37501 ggatgcaaat gtataccagc actttcgggc agatgaacgt acatttatcg attcggtagg
    37561 cgattggatt gaacaggttc aaggacaata tgcaccctat ctgaccgaat ttcttgaccc
    37621 aagacaatcc tatattttag aaacactgat tcgacaaagc agtgatttgc gtttcatgtt
    37681 ttatggcgga tatgaacagg cagaaagaaa aagatgtttg attttccccg attattacga
    37741 acctgtagag gaagattttg aaacagcatt gtatgaaatc cattatccta gcaaatttgc
    37801 cagcctaagt catgggaaaa tccttggaac tttagtggga acggggatca aacgctctta
    37861 ctttggcgat atcatctcgg acggtgatcg ctggcaagta tttattgcaa atgaggtgga
    37921 tcattttgtt gctgcacaag taacaaagat tggaaaggtt gctgtacgct tggaaccaat
    37981 caaatacaca gaaatgatta tgccgaaaga tagctggaca caagaaagag gaacagttag
    38041 ttccatgcga ctagacagtg tgatctctga gatttataat atttccagac agcggtccaa
    38101 gcagctgatc gaagctggta aagtaaaagt gaactggaca gaaaatacaa gacctgattt
    38161 tgtacttgaa ctattagata ttatttcagt cagaggttat ggcagagtcc agatccaaga
    38221 acttgagggg aagacgaaaa aagaaaagta cagggtactg ttcggtgtat tacgtaaata
    38281 aaataaaaag gtggtttact catggcttta actccattag atatccaaaa taagacattc
    38341 ccgacaaaaa tgcggggata taatcaagac gaagtagatg atttcttaga tttagttgtt
    38401 cgtgactatg aggaattgac tcaaagaaat cgagaattag aaaaagcagt caaacattct
    38461 gaagaaaaac ttgagtactt caatgaatta aaagatgctt tgaaccaatc aatcatcgta
    38521 gctcaagata cggctgataa agtaaaaaca agtgcaagca aggaatccga agtgatcgtt
    38581 acttctgcac aaaacaaagc agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa
    38641 ttaacaactg atgcagaaga aaaagcaaga aaaatcttga cagatgcaac agaaaaagca
    38701 agacagcttg cgacagaaac tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt
    38761 atcagcttga tgcttgagtc tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt
    38821 ttacaaccat tctccagcta cgtgactgac agccatgaag tgatcaaaga agttttagca
    38881 aaacagcttg acaatgaaaa tgatactgat gtaaactcag acacaggagt catagaagca
    38941 cctatgacgg catttgatac acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa
    39001 aacgagtaga acagaaaagc actttgttat tcttaaagcg agccgatgag agtggaagtt
    39061 cggtaagaca ctgcatggtg aaagatcctc tacttgagac ctgcttttcc gaaattagag
    39121 taagaagcag cggctgacct cgttattggt cacagagagt ttgcactaga tagatgcaac
    39181 taaatttggg tggtaccacg gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc
    39241 cggaaaacaa ataaaggagt gtatggttga atgaaaatga aagaaacctt gcaattagga
    39301 aaaacaaaat tccctatgcg tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa
    39361 tgggaagaaa atcgaatcta ccaaaaaaga caagagttga acgaaggaaa acctagcttt
    39421 gttcttcatg atggacctcc atatgccaat ggtaatatcc atttaggaca ttcattgaat
    39481 aaaatcagta aagacattat cattcgttca aaatcaatgt ctggctatcg tgcaccatat
    39541 gttccaggat gggatacaca tggattgccg attgaacaag tattggctaa taaaggcgtg
    39601 aaacgtaaag aaatgtccat ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc
    39661 caagtagata agcaacgagc agatttcaaa cgcctaggag ttgcaggaga ttgggaacat
    39721 ccttatgtta cattagatcc ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg
    39781 gctgaaaaag gctatattta caaaggattg aaaccaattt actggtctcc gtcaagtgag
    39841 tcttctttag cggaagctga aattgaatat aaagatgtaa aatctccatc tatttttgta
    39901 gctttcaatg tcaaagacgg caaagatatt cttgatgaag atacagcttt tgtcatttgg
    39961 acgacaacac catggacatt gccagcaaac caaggtattg ctgtaaatcc aaaatttacg
    40021 tatgtattgg tggaagccga tggccgtaag tttgtcgtag cgaaagattt gctagaaaca
    40081 gtccaatcag aaatcggttg ggaaaatgta acagttctaa aagaatttgc tggacaagaa
    40141 atggaatata tgactgcaag tcatccattc tatgaacgtg aatcacttgt gattttaggc
    40201 gatcatgtta ctttagacgc tggtactgga ttagtccata ctgctccagg acatggggaa
    40261 gatgactata tcgctggtag caaatataaa ctatctgtag tgtcacctgt agactcaaaa
    40321 ggattgttta cagaagaagc acctggattt gaaggtatct tttatgacaa agcaaatcct
    40381 atgatcactg atctattaaa agaaaaagga gctttgctga agctagattt ctttactcat
    40441 agttatcctc acgattggcg gacgaaaaag cctgtgatct atcgggcaac tccacaatgg
    40501 tttgcatcta tcgataaatt ccgtcagaat attttggatg aagtagaaaa agtagagtgg
    40561 gttatcccat ggggtaaaac acgtttgtat aatatgatcc gtgaccgcgg cgattgggta
    40621 atttcccgtc agcgtgcatg gggagtacca cttccgattt tctatgctga aaatggagaa
    40681 gcaatcatca caccagaaac aatcgaacat gtggcaaaat tatttgctga acatggatca
    40741 attatctggt ttgaaaaaga agcgaaagaa cttcttcctg aaggatttac ccacccaggt
    40801 tctcctaatg gcgaatttac aaaagaaaaa gatatcatgg atgtctggtt tgattctggt
    40861 tcttctcatg aagctgtttt acgtcagcga ccagaattat cattcccagc agatatgtat
    40921 ttagaaggat cagaccaata tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct
    40981 atcaatggtg tagcaccata caaatcagtt atttctcaag gatttgcttt agatggtgaa
    41041 ggccgtaaga tgagtaaatc tttaggaaac atcattgcac cagaaaaagt gatcaaacaa
    41101 tttggtgcag atatccttcg cttatgggta tctagtgtag attatgaagc agatgttcgt
    41161 gtttctatgg atatcttaag tcaagtggca gaagtatatc gtaaaatcag aaatacgatg
    41221 cgtttcttat tagcgaatac agaagatttc gatcctaaaa aagatactgt ttcgtacaat
    41281 gatttacgtt ctgtagacaa atatatgacc gttcgattga accaagtaat caaagaaatc
    41341 cgagaagaag ggtacgacaa atataacttc atgcatattt accgtacagt tatgaacttc
    41401 ttgacggttg atctatcgtc attctacctg gattttgcta aagatgtagt ttatattgaa
    41461 gcagaagatg attatcaacg tcgttgcatg caaactgttt tctatcaaac agctgtagct
    41521 ttgacaaaat tattgactcc tatcattccg catacagcag aagaaatctg gtcattcctg
    41581 aaagaagaag aggagtacgt tcagttaagc gagtttcctg gttatgaaga gtttgcaaac
    41641 caagaagaat tgatggatac ctggactgct ttcatggact tccgtgataa tgtcttaaaa
    41701 gcattggaag aagcaagaaa cagcaaattg attggtaaat ctttggaagc aaaactaact
    41761 gtttatccaa aacaacaagt tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa
    41821 ttattgatcg tttcaccaga ctactttgaa gttatggcag cagatgaaca agtgccagat
    41881 aacgctatgg tatttgatga tgttgctatt acagtggaaa aagcagacgg ggaaacttgc
    41941 gatcgctgtc gccaagttcg taaagatgta ggagtagatg aaaaacttcc tcatctatgc
    42001 ggtcgttgtg caaaaatcgt tgaagacttc tatcctgaag ctgtagctga aggattcgaa
    42061 gaaaagtaaa agggaaaagg gggtgtgaca tgttctttgt cacaccccct tttgttatca
    42121 atcgtttaat tttccacgtt cacgatacca gttatctggt tgccagatcc attcgaatcc
    42181 gtctttttca agtagttcga aagctgcttt aggtcccatt gatccagctt tgtagttggg
    42241 aaaatccggt tgtgtttggt cccaagcttt tcgaatgaca tccacgatat gccaagattg
    42301 agcgacttct tcccaatgag agaagttcgt tccatcacca ttcaacgcat ctaatagtaa
    42361 tttttcgtaa gcttctgggc tattttcaac catttctgca ctatttctaa attccaattt
    42421 gattggttcc gtttcaaatc cttgccctac ttctttccca ttcaaggata aagaaaatcc
    42481 ttcagttggc tgaatataga tcgttagaac attaggtgga agtgtcgtat cgtcacatgg
    42541 ttcatcgaca gatgttttga atacattaac aggaacttgt ttgaatacga tgttgatccg
    42601 agtccctttt tcagtcaaac gtttgcctgt acgtacatag aaaggaacac ctgaccaacg
    42661 gaagttatcg atagtgaatt tgcctgcgac gaatgtttca gtctctgatt gagcatccac
    42721 tttgtcttct tcgcgatagc ccttgaattg tacaccatcc aattcacctt gagcatattg
    42781 cccgcgaacg aagttttcta acacttcttc ttcgttataa atgcggatag ctttcaatgc
    42841 tttgatcttt tctgtacgga tttctttttc ggaaaatgct actggcggtt ccattgcaag
    42901 taatgctaca acctgtaaaa tatgattttg caccatgtct ttaagtgcgc cactatgatc
    42961 gtagtagccc ccgcgatctt ctacaccgag gttttcgcca aaagtaattt ggatattgtc
    43021 gatatagcga ttgttccatt gagcctcaaa aatattattg gcaaaacgga tagctgagat
    43081 gttttggatc atttcttttc ctaaataatg atcaatcctg aaaatatctt gttctgggaa
    43141 tacttctctg atttgctggt tcaattcaaa agcagactgg taatcagaac cgaacggttt
    43201 ttctatgatc aaacgattga agccattttt tgtcataata ttttgtgatt tcaaatgaga
    43261 aacgatcgtt ccaaaaaatt gaggtgccat agccaaataa tagacacggt taccgtttaa
    43321 ttgatagtgt ccatctaatc ggtcagacaa ttcttttaat gtctgataat gcgttgaatc
    43381 atttacatca tgggattgat agtaaaaatg gcttgcaaag gtagttgctt cttcatctga
    43441 aggattcaat gcacggattg tttctttgac aacttcacgg tagtgctcat cgctccaggg
    43501 acggcgagcg gttccgatta cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg
    43561 gaacaaagac gggtacaatt tacgctgggc taggtcacct gtaccgccga atatcgtaaa
    43621 caatacattt ttttcattag acatgtggat acactccttt atattttatc gacaaaaatc
    43681 gtgctggccg ccttaaagct gacggggatt ttctttctat cattttggat agtgatcggt
    43741 ccttcataag ctgcgatgtc aacgatctca tattcttcgt tgatattcag gtcaatagtg
    43801 actaagtaat ccaacaattc tttttcatca agcactcgtg cgatacgcac ttttgtaccg
    43861 acaggatagg actccaatgt ttgtcggcgt tcttcatgga caacttcctc ttttttagga
    43921 atcgatcctc catgaggaca atgttcagga tgattcaaat agttttctaa atgatctgca
    43981 agatgttccg aagtcacatg ttccaatact tcagcatcat catggacttc attccagcta
    44041 tagtctaaat gttcaactaa aaaaacttcc cataagcgat gtttgcgaat cagactgcta
    44101 gctttttgca gtccgcttgc agtcaattgt acaccttgat aaggggaatg ttctacaagc
    44161 tcttctttca caagtttaga aatcatttca ctgacagatg cagctgagac atctagacta
    44221 gatacgattt gtttattgtt gactttcgtt gtatctccac ctagttctaa aatgattttc
    44281 aaataatctt ctcgatttgg tgtcatattc gatcatcctt tcatggtaaa aaaccatcat
    44341 tagtaagtgt aacaaaaaaa acgttaattg actatttttc taaataaaaa acaaaggatt
    44401 tttagacgtt tttacaacaa aaaacgaaag atgatagaga agactcattc tttcgtttat
    44461 aaaggatata tatatacatt gggagggatc tatttttcag ataacaactt atatgaagtg
    44521 tctgcagatt cgtcataaag agtttgttcg ggataagaaa agataagata aaaaccatac
    44581 tgatcacgta cgactaaacg atattcatct atttctacca cagatgtgcc aagagactga
    44641 ccgttgatca aaaacgtgta atcagatgta atctgtaatt gttgtttttg ctggctatca
    44701 ctaaagaacc atgtaccttg atactttgag agtccttctg acttttccgc ttgttttttc
    44761 tttttcaagt aaataaaact tgagagaacg gtaatgagtg aaccaagaaa atagatcatt
    44821 ttttttatca ccaaatcact cctttatgag aaaataatcg catagacata ttctttcttc
    44881 tttatttcat tgtaccgaat aaaagctaaa atttctctaa gactttcgac tggttttttt
    44941 actgtttttc tacaaattat gataggatac atagtaggaa gaaaggtgac ataatttgaa
    45001 gtatcagcaa gtggaaatag ctttttttat tgaccgcccg aatcggttta tagcacattg
    45061 tatgaaaaaa gacggagaaa taattattgc gcatgtcaaa aatacaggta gaggaaaaga
    45121 ggtcctgata ccaggcgctg aagtggctat tgtatttgct cctggaccta aacggaaaac
    45181 cgcctatgat ctgattgctg taaaaaagaa gggtgactgg tttaatatcg atagtcaatt
    45241 gcctaatcgg ctggcaattg atggtatttt agacggcacg atccttttac caggattaac
    45301 tggtccattt acggtataca agcgtgaagt aacttatctt cactccaaat ttgatattta
    45361 tttagagacg gtggtcggac aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa
    45421 taaagcgatt ggtgcttttc ctgatgcgcc cacactcaga ggattgaaac atataggaga
    45481 attaacgtat gccgcacaag atggttatgc cgcttatgtc ttgtttattg ctcagttcga
    45541 acatcttcat ctggcaacga tccatgaaga aatgcaacca gcactagctg atatggttcg
    45601 acatgcgcag caaagcggag ttcaaatcct cgcctataat tgtcaagtat cggcagatga
    45661 ggtaactgtc aagcaggcca ttccatttga tgtagatatc ccttttgaag atcctataca
    45721 atagaaagaa ggtaaaataa tgatcaactt aggtattatc ggaacaaatt ggattactca
    45781 tcaatttgtc caagcagctt taagcacaaa aaaatatgaa ttgacagcgg tatattctag
    45841 acatttggaa aaagcacagg aatttggttc tcattatgaa ggggatatcg aattcgcaac
    45901 agatttgaag acttttttca atattactca tatggacacc atttatattg cttctccgaa
    45961 tagtcttcac tttagtcaag ctaagcaagc aattttggca ggaaaaaatg tgatagttga
    46021 aaagccagct ttttcgacgc caaaagaaat ggatgaaatc atccatttgg caaatgaaca
    46081 caatgtgtat ttttttgaag cagcaagaaa tatccatgaa tcaggtttta aaaagattgc
    46141 cgattactta ccattaaaag acgagataat cggtgcaaat ttcagctata tgaaatattc
    46201 ttcgcgctat gaccaagtat tagatggaga agaaccgaat atcttctcga ctcatttctc
    46261 aggtggtgca ttgatggatt taggtgttta ccttgtatat gcagctgttg cttggtttgg
    46321 aatgccaaaa gaagtccatt atttcccaag aaagatttct actggtgtag atggtcttgg
    46381 atgggggatc ttacgttatg agaattttga tgtatcgatc cagccaggta aaatcggtga
    46441 ttcttattta ccgtcagaga tttattttga ctcaggaacg ctagtgatga atggagtcaa
    46501 tgcaattgaa caagcacaat ttcatgatcg cgcgcataaa gaaatcattg atttggcggt
    46561 aacagctgca gaaaatccaa tggcagatga agcagaggat tttgccaatg tcatggctca
    46621 tccgaaagat ccagcttggg gattgttgta tgaagagtgg atcgagctag ctagaaatgt
    46681 gaaccaagtg atttatgatt taagaaaaaa tggcggcatc aaattcgatg cagataatga
    46741 ggaagatttt taatgaacga atttgtacaa gtatttcccg aaaattggca gaaaaaatat
    46801 gaggataaag ggtttatctc accttctctt attcaacgag cagtttttca gccactaagt
    46861 aataaacaaa gtatagtagc ggtttctcct acaggttcag gtaaaacact ggcttatctt
    46921 tggcctctac tactaaacgt ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct
    46981 tctcaagaac tagctattca agtagcagat gttgcacgtg aatggggaaa agacaaagag
    47041 ttgaaagtcc aatctttggt gggcggagcc aacgtcaaac gtcagattga gggtttgaag
    47101 gaaaaaccag aaatcttgat tggtacacca ggaagaatat tggaattgat gaaagcaaaa
    47161 aaaataaaag cacatcaagt gaaaacaatg gtatttgatg aagcagacca gctatttgac
    47221 gctgggaaca gccaaatcat tgatcaaatt cttcatcaag ctccaacaga gtatcagcta
    47281 gctttttttt cagcgacagc agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa
    47341 acgtttgaga caattgacgt gacagcagag gacgattctc gaaaaggact gagacattac
    47401 tttttacgtg taccgatacg aaagaaagat gactacttga gacgtctcac gcatatagaa
    47461 gattttcatg gtctgatttt ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa
    47521 ttgctgtata gaggtgtacc ggtaggaagt ctggcctctg atcagaacaa gctactccga
    47581 aaagcagcgc tagaacaatt caaaaagaaa atggtcagag cacttttgac cacagatgtg
    47641 gcggctagag gtttagatat tacaggtctt ccttatgtag tgaatgcaga agttccgtta
    47701 agtgaagaag cttatttgca tcgttcggga agaactggtc gaatgggtaa tgaaggaata
    47761 gtaattacac tcgtacaaga taataatcta agagatttaa aacggctttt acgtccatca
    47821 aatataacac ttgaagagat ttttttatac ggaggtaagc tgcaaactga gcagccaacc
    47881 aaagaagatg cagctcctgc agtaaataaa aagtatgcga aaactttttc tggaaaggag
    47941 cagaacccag aatataacaa agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa
    48001 gagagaaata aaggagcaag gcgcaaataa gcgccttttt atgctatgat tatgaaaacg
    48061 ggaacaaaat tggttgccag aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc
    48121 agctattgac agaaaaccaa caaggattga cagcaagtga tgtagctgag cgcttaacta
    48181 tcgatcgaag caatgcaagc cgatatttaa gtgaactata caaggcccat catattgtaa
    48241 aaaccgctgg ccgaccagtc gtttactctt tgccaacgga aaagtcaaaa tctgatgaag
    48301 tgcatgtcga ctcttctacg caagtgactt ttgaaacact cgttggagaa aatgattctt
    48361 taaaagtcag catacagcag gcaaaagctg cgattcttta tccaccgcgg gggttgcata
    48421 cgattatttt tggagaaact ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt
    48481 ttgcaatcga ttcagagatg ctttctgcag atgcgccatt tgtttctttc aattgtgcgg
    48541 attatgctca aaatccgcaa ctgctctttg gacacatctt tgggatcaaa aaaggggctt
    48601 acacaggagc agctcaagat agtcccggtc taatagccaa agcagacggc ggtatattgt
    48661 ttttagatga gatccaccgt ttaccgccgg aagggcagga aatgctgttt actttcattg
    48721 ataaaggtat ttaccgtccg cttggagaaa gtgcacaagt acatgaagca tctgttcaga
    48781 tcattggggc gacgactgaa tcttcagaaa gtttcttatc tacgttcaat cgacggattc
    48841 ctatggcaat caccttgccg aatcttgctt cacgttcact ggatgagcgt tatgaaatca
    48901 tctctctttt tatcaaacaa gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag
    48961 aagcgattct tgccttcatg ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag
    49021 acttgaaact agtctgtgca aaatcttttt tacattaccg tactcatcat gagaaaaagc
    49081 taatcattcg aaaagaagaa ctttcccttc aagtacaaaa aggattactg aaaatcaagg
    49141 aaataccgga acgtctcgac cgattcatgg acagcaaaag tcagtatttg acatttgaac
    49201 caggatttgc tgatgttgtc tggtcacaag atccagaacg aaacatgcag gtctataacg
    49261 atattgagga aaaagtcact tctctttccg aaaccggtgt ggagaacatt gatctagaga
    49321 ccctcatttc aaaagatgtg gatgcttatt tccagacata tgtcaaagaa ttgactaaaa
    49381 gttcgattcc caaagatctt ttgccagatg aaatctggcg gttaaccaat caattgtatg
    49441 atgtggctga aaaacggtta gaccgtattt ataatgaaaa agctcgtttt gcttttgctt
    49501 tgcatctgca aagtacattg gatcgtgtga gagaaggtca tatgatcgtc cacccagatt
    49561 tgaataacgt acggaaaaat tttaagaggg aattccaggt agcaatcgat ctttcaacga
    49621 tcattgaaga agaagaacaa gttgagattc cttttgatga aatcggcttt attagtatgt
    49681 ttctttctat caatctagga gaaagtgagc ctgttaaaga aaacaaagta gatgttctca
    49741 tattgatgca cggcagacag actgcttcaa gcatgttgga gatggcacaa gaactattag
    49801 gaacaaaagt agggaaagct tttaatatgc cactggttat ggaagttcaa acaatgtatg
    49861 aagaagtgtt acaatatgtc aaaaactatc aagagcattt gacgaatggc ctactcttgt
    49921 taacagatat gggatcatta aacaccttca gtgatttgat ttatgaagag actggcattc
    49981 ggacgaaagc tatcacgatg acaagtacca tgattgtgat agaagcgata cggatggcaa
    50041 gtgttggaag aagcttagaa gatatttatc aaaatatcca gctttccttt gaatcgatcg
    50101 tacgtgatca gttcaaagcg gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg
    50161 tgacatgctt tactggtgaa ggagtagctg ccaaattata tcagcgtatt atgccagtga
    50221 ttaatcaaaa taaggtagaa ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa
    50281 aacacatcga tgccttattg gaagaatatg agatcaaagc aattgcggga acagttgatg
    50341 ttgattatca aaatatccca tttttttcag cctatgacgt ttttgatgat gaaaaactaa
    50401 atattttaaa gcgtatagct gatgatgaga tcccgacagc taaaattgtc gattcacttg
    50461 ctggatcatt aagcgatgtc ttttctattg atgaactgat ccgttcattg caaaagacag
    50521 ttcagcagat ccagaccgat ttgtcgatta tcgtggaacc tggagtagat actggaatcg
    50581 ttatccattt agccttcatg ttagacggat tgatcaaaca agaaagccat cggcaatttg
    50641 agaaactgtc tattttctca aaaatttatc gtttggaaat ggatatcgtt cgggcaaact
    50701 tgatggcact agaacgaaaa tatcaggtaa aaattccaga agatgatatc gcatttctta
    50761 cacaaatgtt tcttgaaaat aaaataaaaa aatgaacaca ctaatcatac tgtttacaca
    50821 ctaaacatct gtgtgcgcac tataaaaaag agctaaaaag catgaaaacg ctatttcagc
    50881 tctttttttt attggcatgc ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg
    50941 ttaaaaatgg caaaaaaaac aatcatgtta gtttgttcag cagggatgag tacgagttta
    51001 ttggtcacaa aaatgcaaaa agcagcagaa gaaaaaggga tggaagccga tatttttgcg
    51061 gtctctgcct cagatgcaga taataattta gaatctaaga atgtcgatgt attattatta
    51121 ggtcctcaag tacgtttcat gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt
    51181 ccattagatg tcatcaatat gcaagactat ggcatgatga acggagaaaa agttcttgca
    51241 caagcagaaa atctaatgaa ataaatcaag tatgcagttg ggggtataac aacgtggaag
    51301 atcaacaaaa tctagaagcc atcatggggt taatcatgtt tggcggtaat gcaaagagcg
    51361 atgcaatgga agcgattgcg gctgctaaaa tcggtgattt tgagttagcg gataggaaaa
    51421 tcgctgatgc agaagaatca cttatacaag cgcatcatgc acaaacagac atgttgacac
    51481 aagaagcaca aggaaatcat atgcaagtga cattattgac tgttcacagt caagatcact
    51541 taatgacatc tattgcgttt actgatttag caaaagaaat catcgaactt tatcgtcgcg
    51601 tatacagcga ataaatacaa cttctttttt gaacggaaga agaaaaattt ttgaacggat
    51661 tttttatttt gttataagga gaactctctt tatataaaaa acttatagga ggaagtaaaa
    51721 aatgaacgga ctatcaagtt ggatggagaa atacatcctg ccggttgccg caaaaatcgg
    51781 agcgcaaaaa catttagttg ctttgcgtga tgcttttatc ggcatactgc ccgcaacaat
    51841 ggcgggatca gtcgctgtaa tgatcaatgc aattattcgt gatttaccgc aacagtttaa
    51901 cagtacctat gctgacagtt tagcagcaga caaaggaatc tttgccgtat tgaatgtcat
    51961 catcggaatc aatggttttg tctggaacgg tacactagca atcgccggat tgatctttgc
    52021 cttttcttgg gggtataatc ttgcccgagc ttatggagta aatgatctag ctggggggat
    52081 cgttggttta gctaccttga ttcaaggtat ttcctttggt ccagcattat cagcagcatt
    52141 agatgtgaat gttccttcaa atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac
    52201 gattgcagat ggcacactgt cagttggatt atggggttgg ctaaaactag atcatttgaa
    52261 tggaaacgct tattttacag ttatgatcat gggagctatt tcagtcatta ttttttgtaa
    52321 attgatgctt gccaacatta cgatcaaaat gcctgattca gtaccaccag cagtatctaa
    52381 agcatttgca gccattttac cagcgacgat cgctttatac gtcattgcta ttatcaatta
    52441 tatcgtaggt cgtgtagctg atgggcaact gatcattgat ttggtgcaaa aatatattgc
    52501 agaaccattc ctagggttat ctcaagggtt gggagcagta ttgatcgtca cagtatttgt
    52561 tcaagtattc tggttctttg gtattcatgg atcaaatgta ttggcaccag tgctagaggg
    52621 aatctgggga caagcacaac taatcaatat tgatattttc caaaaaggtt ataacggaaa
    52681 aactggtaca ccagccgtgt tagatgcaat cgaagatgga aaagcttata tgtgggtccg
    52741 cggatcgttc gatgcttttg catggtttgg cggatcaggc ggaacgatcg tcttactgat
    52801 agctatcttg attttctcta aacgtgcaga ttatctaaca gtagctaaat tatccattgg
    52861 gcctggtatc ttcaatatca acgaaccagt gatgtttggt ctgccaatcg tattgaatgc
    52921 aatcatgttt atcccattct tgattgctcc agtagtagca acagcaattg gatggacagc
    52981 tacttatctt ggcctagtcg ctccggtatc gcagcaagtc acatgggtag taccgccgtt
    53041 cttattgtca ttcttagcaa caggagcgga ttggcgggca ccgatcgtaa cattagtatg
    53101 tatggtcgtg actttcttga tttgggcacc atttgtcatc tctgcaaaca aaatggaccc
    53161 tgccgcaggc gaacaacaat aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc
    53221 tcataatgaa ttttcgagga ggaactgaaa tgattacaat tgccagtgca atgcaacaag
    53281 atgaaatcaa agaattgtta gaaggataca atgaagaagg aatgactttt acatttaaag
    53341 aaaaacaagg aatcaagctg ttctttgaaa caagtgcaga agatccggaa gttgcggcta
    53401 aaaaagcaaa ggaactaatc aaagcacagc cttggggaac agtcctttat tttcaatcaa
    53461 cagctgttta gaaaatggtt taaaaagata ggatggttga gatgataaaa aacggtttat
    53521 ttttatgtag tattggttct ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt
    53581 atgatttgta tagtatggtc acaggtcttt ttctaacagg attaggaatc tatttcttca
    53641 tgaaggggaa aaaaagagaa gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa
    53701 attaaatatt ccagcatcgt ttttttacga taaagttatg gactctgtcc ttttcgatgt
    53761 acgtaaagcg accggcaaat cagtaactag aaaacagctg aaaaatttgg agtacgtcaa
    53821 acaattctcg caaaataacc gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc
    53881 ttataagttt cgtacatcga caacgaagaa tgaattcgtc gttcattatc aaatcaaacc
    53941 attggacgat tcgacctgtg aggttcatta cacagaaaaa atggagtctt atggcatgct
    54001 tcaaaagttg aatgatatgc ttttaggaac aatcttgatg tattttaaac ggagacgttt
    54061 caaaaaaatg ctagatatga tcgaagcaac gtattaaatc aatgaaacat ggtactattt
    54121 agttattgaa aaatgaagta agctagaagg aatttttcat aggaagatag atctttcctg
    54181 cgaatcctca ctagcttttt tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag
    54241 ctaggtgtat cttcgattga ggaaatgttt gatttagcca cttatgcatt taatggggca
    54301 gatacacctg aaaggcgaga acgttttcgt acacttgcgg agaattcttg gaattacgga
    54361 ttttttgatg aggaggatcg gctaacaagt caagtcatgg cgaccccttt tccagtgaat
    54421 ttttacggac aagagtacct aatggccggc attggctatg ttgcttctta tccagaagca
    54481 aggggacaag gcgggatcaa tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt
    54541 aaagtaagct tatcttatct ggcgccattc tcctatccct tctatcggcg ctatggctat
    54601 gaacaatcat ttgataaaat cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa
    54661 aaaaaatctg gaaaaataaa gcgtatggat tttgaagatg caaaagctgc aatcagaaga
    54721 atttatgaac agatgccgga aaaccaacga gctggtctga agcgcgaaga ctggtggtat
    54781 acgtataaat tcaagcaaaa aggcaattac cagtttgctt tgcattttga tgataatgag
    54841 gaagcaacgg gatatatcgt ttatcagctt gcaacgtctt cctttattat tgcggagtgg
    54901 ggatttttgg atcatgatgc tttttgcagc cttgttcgct tcgtaagttc tcacaatggt
    54961 gcttttgaaa catttgaata ttcatgcggc tacggcggta atgatcaaaa ttatctatta
    55021 gaaaatccat ttgcttctgt aatgatcaca ccttatatga tggcgaggat cgtagatatt
    55081 tctttgtttt tggaaaatta tccatttaaa aagagacaag ctgcatttgc cctacaaata
    55141 gaagaagatc attatgcgaa atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga
    55201 aaaaatcatc tctcaaaagt agaacaaaca aaactcccaa tcgttcgagg aagtatccaa
    55261 catatcacgc agctattatt agggtatcgt aaagtggaag agttggtatt ccaagaaaga
    55321 atacaggtga caaaagaata tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca
    55381 attcttaatg actatttttg ataaaaaacg ggtgatgaca atatttttgt caccaccctt
    55441 ttgtttttca tttggcattt gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt
    55501 tgaatttttt gttttctttt gttttagggt tttctttttt gtggtcttgg attgcttctt
    55561 tcgtaatctt ttcccgattt tttttagatg aatctaagcg ttctgcgtat actttttctt
    55621 ttggcttatc attcattgga aaggacctcc ctacattatt tcattctacc tctagtttat
    55681 catctgttaa ggactagtgg aaacgttttg cctaacttat aagctgattt ttttagcaga
    55741 taagcttgca agtaaaaaac aaacaagcta caattgattt attgaaattg ttcctgtagc
    55801 tcagtaggat agagcaatcg cctcctaagc gataggtcgc tggttcaatt ccagtcagga
    55861 acgtataaat aacccctgta tcctttgcgg tacaggggtt tgttttgtat ttgtgccaaa
    55921 aatgtgccaa actagttatc atttttatat aaactatcga acacagtatt gattttttct
    55981 ttatcctgat cttcaagctc tcgaacaata tgaagataag tagacat
//
LOCUS       DAZUMT010000003        54697 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000003 DAZUMT010000000
VERSION     DAZUMT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.003"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..726)
                     /locus_tag="ACG8FM_000165"
     CDS             complement(61..726)
                     /locus_tag="ACG8FM_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS5092861.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACG8FM_000166"
     CDS             complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACG8FM_000166"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS5092862.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACG8FM_000167"
     CDS             complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACG8FM_000167"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS5092863.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACG8FM_000168"
     CDS             complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACG8FM_000168"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS5092864.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3040..3360
                     /locus_tag="ACG8FM_000169"
     CDS             3040..3360
                     /locus_tag="ACG8FM_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS5092865.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3472..4329)
                     /locus_tag="ACG8FM_000170"
     CDS             complement(3472..4329)
                     /locus_tag="ACG8FM_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5092866.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(4437..5741)
                     /locus_tag="ACG8FM_000171"
     CDS             complement(4437..5741)
                     /locus_tag="ACG8FM_000171"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5092867.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5738..6247)
                     /locus_tag="ACG8FM_000172"
     CDS             complement(5738..6247)
                     /locus_tag="ACG8FM_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092868.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6422..8557)
                     /locus_tag="ACG8FM_000173"
     CDS             complement(6422..8557)
                     /locus_tag="ACG8FM_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5092869.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(8640..10295)
                     /locus_tag="ACG8FM_000174"
     CDS             complement(8640..10295)
                     /locus_tag="ACG8FM_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5092870.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(10297..10611)
                     /locus_tag="ACG8FM_000175"
     CDS             complement(10297..10611)
                     /locus_tag="ACG8FM_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5092871.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            10781..11041
                     /locus_tag="ACG8FM_000176"
     CDS             10781..11041
                     /locus_tag="ACG8FM_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS5092872.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11055..11369
                     /locus_tag="ACG8FM_000177"
     CDS             11055..11369
                     /locus_tag="ACG8FM_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS5092873.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(12099..12653)
                     /locus_tag="ACG8FM_000178"
     CDS             complement(12099..12653)
                     /locus_tag="ACG8FM_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS5092874.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(12779..14708)
                     /locus_tag="ACG8FM_000179"
                     /pseudo
     CDS             complement(12779..14708)
                     /locus_tag="ACG8FM_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(15014..15730)
                     /locus_tag="ACG8FM_000180"
     CDS             complement(15014..15730)
                     /locus_tag="ACG8FM_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5092875.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACG8FM_000181"
     CDS             complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACG8FM_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS5092876.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACG8FM_000182"
     CDS             complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACG8FM_000182"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS5092877.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(19200..20660)
                     /locus_tag="ACG8FM_000183"
     CDS             complement(19200..20660)
                     /locus_tag="ACG8FM_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5092878.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(20650..21945)
                     /locus_tag="ACG8FM_000184"
     CDS             complement(20650..21945)
                     /locus_tag="ACG8FM_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5092879.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(22277..22954)
                     /locus_tag="ACG8FM_000185"
     CDS             complement(22277..22954)
                     /locus_tag="ACG8FM_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS5092880.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22984..24075)
                     /locus_tag="ACG8FM_000186"
     CDS             complement(22984..24075)
                     /locus_tag="ACG8FM_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS5092881.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(24176..24610)
                     /locus_tag="ACG8FM_000187"
     CDS             complement(24176..24610)
                     /locus_tag="ACG8FM_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5092882.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(25194..26651)
                     /locus_tag="ACG8FM_000188"
     CDS             complement(25194..26651)
                     /locus_tag="ACG8FM_000188"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5092883.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(26648..28543)
                     /locus_tag="ACG8FM_000189"
     CDS             complement(26648..28543)
                     /locus_tag="ACG8FM_000189"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5092884.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(28601..30376)
                     /locus_tag="ACG8FM_000190"
     CDS             complement(28601..30376)
                     /locus_tag="ACG8FM_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5092885.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(30426..31922)
                     /locus_tag="ACG8FM_000191"
     CDS             complement(30426..31922)
                     /locus_tag="ACG8FM_000191"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5092886.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(32116..33738)
                     /locus_tag="ACG8FM_000192"
     CDS             complement(32116..33738)
                     /locus_tag="ACG8FM_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5092887.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(34066..35379)
                     /locus_tag="ACG8FM_000193"
     CDS             complement(34066..35379)
                     /locus_tag="ACG8FM_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5092888.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(35624..35848)
                     /locus_tag="ACG8FM_000194"
     CDS             complement(35624..35848)
                     /locus_tag="ACG8FM_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092889.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35868..36290)
                     /locus_tag="ACG8FM_000195"
     CDS             complement(35868..36290)
                     /locus_tag="ACG8FM_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5092890.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(36432..37142)
                     /locus_tag="ACG8FM_000196"
     CDS             complement(36432..37142)
                     /locus_tag="ACG8FM_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS5092891.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(37160..37657)
                     /locus_tag="ACG8FM_000197"
     CDS             complement(37160..37657)
                     /locus_tag="ACG8FM_000197"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5092892.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(37694..38146)
                     /locus_tag="ACG8FM_000198"
     CDS             complement(37694..38146)
                     /locus_tag="ACG8FM_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5092893.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(38281..38886)
                     /locus_tag="ACG8FM_000199"
     CDS             complement(38281..38886)
                     /locus_tag="ACG8FM_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5092894.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(39336..39956)
                     /locus_tag="ACG8FM_000200"
     CDS             complement(39336..39956)
                     /locus_tag="ACG8FM_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5092895.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39958..40827)
                     /locus_tag="ACG8FM_000201"
     CDS             complement(39958..40827)
                     /locus_tag="ACG8FM_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5092896.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40850..41257)
                     /locus_tag="ACG8FM_000202"
     CDS             complement(40850..41257)
                     /locus_tag="ACG8FM_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5092897.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(41276..42565)
                     /locus_tag="ACG8FM_000203"
     CDS             complement(41276..42565)
                     /locus_tag="ACG8FM_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5092898.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(42592..42894)
                     /locus_tag="ACG8FM_000204"
     CDS             complement(42592..42894)
                     /locus_tag="ACG8FM_000204"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5092899.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42919..45012)
                     /locus_tag="ACG8FM_000205"
     CDS             complement(42919..45012)
                     /locus_tag="ACG8FM_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5092900.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(45501..46265)
                     /locus_tag="ACG8FM_000206"
     CDS             complement(45501..46265)
                     /locus_tag="ACG8FM_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS5092901.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(46376..47245)
                     /gene="mdcB"
                     /locus_tag="ACG8FM_000207"
     CDS             complement(46376..47245)
                     /gene="mdcB"
                     /locus_tag="ACG8FM_000207"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS5092902.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(47270..48346)
                     /locus_tag="ACG8FM_000208"
     CDS             complement(47270..48346)
                     /locus_tag="ACG8FM_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5092903.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(48370..48963)
                     /locus_tag="ACG8FM_000209"
     CDS             complement(48370..48963)
                     /locus_tag="ACG8FM_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS5092904.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48967..50643)
                     /gene="mdcD"
                     /locus_tag="ACG8FM_000210"
     CDS             complement(48967..50643)
                     /gene="mdcD"
                     /locus_tag="ACG8FM_000210"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS5092905.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(50633..50932)
                     /locus_tag="ACG8FM_000211"
     CDS             complement(50633..50932)
                     /locus_tag="ACG8FM_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS5092906.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(51034..52680)
                     /gene="mdcA"
                     /locus_tag="ACG8FM_000212"
     CDS             complement(51034..52680)
                     /gene="mdcA"
                     /locus_tag="ACG8FM_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS5092907.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(52687..53598)
                     /locus_tag="ACG8FM_000213"
     CDS             complement(52687..53598)
                     /locus_tag="ACG8FM_000213"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5092908.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            53718..54575
                     /locus_tag="ACG8FM_000214"
     CDS             53718..54575
                     /locus_tag="ACG8FM_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5092909.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT        15382 a        11750 c         9328 g        18237 t
ORIGIN      
        1 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
       61 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
      121 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
      181 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
      241 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
      301 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
      361 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
      421 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
      481 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
      541 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
      601 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
      661 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
      721 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
      781 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
      841 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
      901 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
      961 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     1021 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     1081 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     1141 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     1201 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     1261 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     1321 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     1381 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     1441 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     1501 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     1561 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     1621 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     1681 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     1741 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     1801 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     1861 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     1921 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     1981 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     2041 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     2101 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     2161 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     2221 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     2281 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     2341 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     2401 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     2461 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     2521 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     2581 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     2641 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     2701 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     2761 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     2821 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     2881 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     2941 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     3001 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     3061 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     3121 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     3181 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     3241 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     3301 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     3361 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     3421 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     3481 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     3541 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     3601 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     3661 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     3721 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     3781 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     3841 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     3901 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     3961 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     4021 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     4081 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     4141 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     4201 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     4261 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     4321 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     4381 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     4441 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     4501 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     4561 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     4621 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     4681 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     4741 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     4801 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     4861 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
     4921 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     4981 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     5041 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     5101 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     5161 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     5221 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     5281 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     5341 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     5401 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     5461 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
     5521 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     5581 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     5641 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
     5701 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
     5761 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
     5821 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
     5881 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
     5941 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
     6001 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
     6061 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
     6121 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
     6181 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
     6241 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
     6301 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
     6361 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
     6421 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
     6481 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
     6541 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
     6601 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
     6661 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
     6721 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
     6781 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
     6841 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
     6901 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
     6961 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
     7021 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
     7081 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
     7141 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
     7201 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
     7261 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
     7321 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
     7381 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
     7441 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
     7501 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
     7561 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
     7621 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
     7681 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
     7741 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
     7801 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
     7861 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
     7921 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
     7981 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
     8041 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
     8101 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
     8161 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
     8221 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
     8281 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
     8341 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
     8401 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
     8461 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
     8521 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
     8581 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
     8641 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
     8701 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
     8761 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
     8821 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
     8881 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
     8941 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
     9001 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
     9061 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
     9121 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
     9181 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
     9241 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
     9301 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
     9361 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
     9421 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
     9481 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
     9541 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
     9601 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
     9661 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
     9721 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
     9781 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
     9841 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
     9901 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
     9961 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    10021 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    10081 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    10141 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    10201 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    10261 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    10321 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    10381 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    10441 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    10501 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    10561 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    10621 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    10681 tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    10741 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    10801 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    10861 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    10921 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    10981 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    11041 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    11101 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    11161 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    11221 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    11281 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    11341 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    11401 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    11461 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    11521 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    11581 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    11641 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga
    11701 agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt
    11761 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa
    11821 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    11881 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    11941 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
    12001 cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt
    12061 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
    12121 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
    12181 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
    12241 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
    12301 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
    12361 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
    12421 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
    12481 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
    12541 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
    12601 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
    12661 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
    12721 atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc
    12781 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
    12841 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
    12901 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
    12961 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
    13021 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
    13081 gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg
    13141 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
    13201 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
    13261 ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    13321 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    13381 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    13441 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    13501 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    13561 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    13621 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    13681 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    13741 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    13801 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    13861 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    13921 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    13981 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    14041 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    14101 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    14161 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    14221 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    14281 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    14341 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    14401 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    14461 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    14521 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    14581 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    14641 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    14701 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    14761 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    14821 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    14881 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    14941 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    15001 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    15061 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    15121 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    15181 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    15241 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    15301 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    15361 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    15421 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    15481 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    15541 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    15601 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    15661 catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat
    15721 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    15781 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    15841 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    15901 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    15961 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    16021 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    16081 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    16141 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    16201 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    16261 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    16321 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    16381 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    16441 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    16501 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    16561 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    16621 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    16681 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    16741 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    16801 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    16861 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    16921 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    16981 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    17041 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    17101 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    17161 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    17221 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    17281 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    17341 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    17401 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    17461 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    17521 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    17581 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    17641 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    17701 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    17761 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    17821 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    17881 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    17941 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    18001 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    18061 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    18121 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    18181 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    18241 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    18301 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    18361 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    18421 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    18481 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    18541 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    18601 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    18661 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    18721 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    18781 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    18841 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    18901 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    18961 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    19021 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    19081 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    19141 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    19201 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    19261 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    19321 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    19381 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    19441 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    19501 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    19561 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    19621 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    19681 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    19741 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    19801 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    19861 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    19921 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    19981 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    20041 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    20101 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    20161 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    20221 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    20281 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    20341 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    20401 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    20461 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    20521 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    20581 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    20641 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    20701 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    20761 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    20821 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    20881 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    20941 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    21001 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    21061 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    21121 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    21181 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    21241 acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    21301 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    21361 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    21421 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    21481 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    21541 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    21601 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    21661 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    21721 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    21781 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    21841 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    21901 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    21961 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    22021 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    22081 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    22141 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    22201 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    22261 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    22321 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    22381 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    22441 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    22501 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    22561 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    22621 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    22681 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    22741 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    22801 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    22861 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    22921 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    22981 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    23041 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    23101 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    23161 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    23221 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    23281 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    23341 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    23401 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    23461 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    23521 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    23581 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    23641 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    23701 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    23761 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    23821 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    23881 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    23941 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    24001 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    24061 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    24121 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    24181 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    24241 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    24301 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    24361 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    24421 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    24481 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    24541 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    24601 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    24661 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    24721 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    24781 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    24841 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    24901 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    24961 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    25021 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    25081 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    25141 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    25201 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    25261 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    25321 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    25381 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg
    25441 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    25501 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    25561 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    25621 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    25681 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    25741 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    25801 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    25861 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    25921 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    25981 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    26041 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    26101 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    26161 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    26221 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    26281 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    26341 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    26401 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    26461 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    26521 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    26581 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    26641 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    26701 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    26761 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    26821 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    26881 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    26941 ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa
    27001 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    27061 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    27121 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    27181 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    27241 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    27301 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    27361 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    27421 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    27481 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    27541 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    27601 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    27661 ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac
    27721 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    27781 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    27841 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    27901 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    27961 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    28021 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    28081 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    28141 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    28201 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    28261 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    28321 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    28381 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    28441 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    28501 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc
    28561 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    28621 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    28681 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    28741 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    28801 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    28861 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    28921 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    28981 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    29041 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    29101 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    29161 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    29221 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    29281 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    29341 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    29401 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    29461 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    29521 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    29581 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    29641 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    29701 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    29761 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa
    29821 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    29881 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    29941 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    30001 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    30061 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    30121 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    30181 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    30241 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    30301 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    30361 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    30421 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    30481 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    30541 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    30601 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    30661 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    30721 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    30781 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    30841 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    30901 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    30961 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    31021 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    31081 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    31141 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    31201 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    31261 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    31321 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    31381 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    31441 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    31501 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    31561 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    31621 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    31681 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    31741 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    31801 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    31861 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    31921 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    31981 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    32041 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    32101 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    32161 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    32221 tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    32281 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    32341 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    32401 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    32461 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    32521 cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa
    32581 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    32641 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    32701 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    32761 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    32821 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    32881 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    32941 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    33001 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    33061 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    33121 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    33181 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    33241 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    33301 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    33361 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    33421 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    33481 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    33541 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    33601 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    33661 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    33721 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    33781 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    33841 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    33901 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    33961 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    34021 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    34081 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    34141 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    34201 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    34261 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    34321 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    34381 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    34441 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    34501 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    34561 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    34621 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    34681 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    34741 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
    34801 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    34861 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    34921 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    34981 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    35041 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    35101 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    35161 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    35221 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    35281 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    35341 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    35401 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    35461 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    35521 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    35581 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    35641 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    35701 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    35761 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    35821 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    35881 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    35941 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    36001 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    36061 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    36121 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    36181 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    36241 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    36301 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    36361 tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta
    36421 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    36481 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    36541 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    36601 agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    36661 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    36721 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    36781 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    36841 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    36901 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    36961 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    37021 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    37081 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    37141 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    37201 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    37261 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    37321 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    37381 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    37441 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    37501 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    37561 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    37621 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    37681 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    37741 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    37801 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    37861 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    37921 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    37981 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    38041 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    38101 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    38161 attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa
    38221 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    38281 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    38341 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    38401 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    38461 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    38521 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    38581 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    38641 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    38701 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    38761 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    38821 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    38881 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    38941 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    39001 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    39061 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
    39121 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    39181 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
    39241 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
    39301 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    39361 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    39421 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    39481 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    39541 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttgc
    39601 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    39661 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    39721 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    39781 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    39841 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    39901 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    39961 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    40021 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
    40081 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    40141 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    40201 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    40261 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    40321 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    40381 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    40441 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
    40501 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
    40561 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
    40621 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    40681 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
    40741 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    40801 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    40861 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    40921 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    40981 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
    41041 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
    41101 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    41161 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    41221 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    41281 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    41341 catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact agcaaagcgg
    41401 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    41461 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    41521 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    41581 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
    41641 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    41701 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
    41761 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    41821 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    41881 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    41941 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    42001 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    42061 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    42121 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    42181 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    42241 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    42301 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    42361 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
    42421 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    42481 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    42541 cgatgataaa attgataacg cccatgtttt taccctcttt tctttttgta tttagataac
    42601 tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg aaacaatatt
    42661 tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa
    42721 attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa tatcacaatg
    42781 gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc
    42841 aaaactactt cctaaacctg caccgcatac gaataaaatg ttcaatttgt tcatctttgt
    42901 tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt tgaaatcttc
    42961 gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca attcaattag
    43021 ttctttcatg atgttcagat gcgagtagga gtctactgct gccaaacaaa aaataatctt
    43081 tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag ctacgctgac
    43141 tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt gtttgccaat
    43201 cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat agcgctcttc
    43261 gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct cccaagtagt
    43321 ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca taggctggat
    43381 ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact tgccatagat
    43441 gattcgatct actcggccac cactttcttt catgacatct attatttgag tgaataactc
    43501 cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc
    43561 ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa gcggcttatt
    43621 tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat ctactaattc
    43681 gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt ctttcagatt
    43741 ctctgctaac agatttccag tagccatccc atggttacag acaacgactg atttataaac
    43801 ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga caagaaaagc
    43861 tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa caaaatgagt
    43921 gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg gattcatgac
    43981 ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga gattttcgaa
    44041 taaagcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa attggatcaa
    44101 ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat ctctagcatt
    44161 gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat tgtccaattg
    44221 aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta tttctgtatt
    44281 tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc agatcaatgt
    44341 aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact tattggaata
    44401 gatctcctgt atttttttga tttccaccaa aggaaaataa tggagaaaga ttttttgagc
    44461 cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat tctgattgat
    44521 aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca gtccttgctt
    44581 tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc gtctcatatc
    44641 ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta gatggatcgg
    44701 gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc gattcaaaaa
    44761 gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct tttccagctt
    44821 caacaaaaaa cctttgttgc gaattgtacg gatctccgga agctttttac tgctcaacca
    44881 gatattcgct tcaagaactt cctgacggat cgtccgctga ctgacattga actcacttgc
    44941 tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta tttcaattgt
    45001 tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg cttacataat
    45061 aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca aacaaaaaat
    45121 aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat tacaaaaatt
    45181 ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    45241 aactaaacta aaatagtgct tcctattttt cgtttggttg ttcttattat cgcagatgca
    45301 agcttttaaa caccatttag tggagaggtt gtgacagaga tgttcagcct tgagcgataa
    45361 gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat tgccgatagg
    45421 ctatctctgg cacaccgttt attcagaaaa aggagctgcg acaaggcttt tgtcacaact
    45481 ccgttcataa atatttcctg tcaatccagc ataatcggct gaataaaatc ggcttgccat
    45541 cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg tcgttgtgtc
    45601 ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca gcatctgaaa
    45661 tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac ttcctgaata
    45721 gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag aactttctga
    45781 tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt ttcttcttcc
    45841 caagcggatt ggatcttcat gaatatcttt tggatttcca gaaactgtgc tttcttttct
    45901 ttagccgttc ctgacatgcg ggacaatatc tccgctttca gccttttctt ttgtctttta
    45961 ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa tgcaagaaat
    46021 cccgtttgct cataactaga aggacctgta cctggaccat aatacccata agaatctctg
    46081 ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccaccaaa tgaacctctg
    46141 cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc gaatccttgg
    46201 gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata attgattttt
    46261 ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatgata gccttgctaa
    46321 aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt cttatttagt
    46381 cattgagtaa catcggacaa atgtatgtgt cggcagtcca taattcctct atcttcagca
    46441 tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg ttttcctgac
    46501 aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta gcagcatgag
    46561 caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg catgtgtcat
    46621 ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat gaatcattat
    46681 ctagctcctt agctgcttga attgcaagcc tgatatgcgg ataccctaat atcgcttctt
    46741 catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg gtttttttat
    46801 gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt attttcgata
    46861 aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat agccccattg
    46921 cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt gccttttccg
    46981 cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca tgatgccatg
    47041 ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta agaaataaag
    47101 catagtccat atcggaatgt gcacccgcat ccagtgcatc cactaatcca ggttttggac
    47161 ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc agcgagttgc tgagtcactg
    47221 ctttttttcg ttcgtcattt atcatgacac tcactccttc ttcctactat tacttgctga
    47281 aacctttttt gatagctacc caaatgatat gaagtaacca gcttaaagca aatgcacaac
    47341 ttgccgtcat tagataagct acaagaatca tgactcctga aggtaactgc agccaactat
    47401 ctttaaacaa taataacagt acttggaaaa caaatacatt ggctaaatac gtacgataag
    47461 cataaacaga caaccatttg aacaatggca attttggcga atcgtttaca atcattttgg
    47521 aagcgcctat gaagacagca aagatgatga ctaaactgta taaactttgg atcgtattca
    47581 agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc gagcaatgaa cggacagaaa
    47641 agaacgcgat aactaaggca acaggtaaga aaagataacg tactctgtcc aaatattgat
    47701 agactttttc gtgataaaca aatgcggcta ccccataaat catgaaacta gcaacaaacc
    47761 gatctaataa gtaccagctg tcatgataag gcccagtcaa tacttgtgtt tgatagaaca
    47821 gataccatgc gacatataaa atcgttgaac caatcaatac tggccataca tattttttat
    47881 tagcaaatac tttgtaaccg agccaaacaa aaaacggcat gaacaattgg atctgcagca
    47941 tcataacggt ataccataaa tgaggcgctc catcacctgt gacatatttc agtaaaaagc
    48001 ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg aaatagccaa agataagccg
    48061 tcgtccatag aagatacgga acgactagtt caaaaaactt ttctttcaaa aattcccaat
    48121 aagacaaatg ctgacttgtt tttaccatat tataaacaat tgcaaaaata aacattggtg
    48181 ctgtgtattt aacgatgacg taaaaggtac caagaaaagc tgtatttgtt gcgctgtggc
    48241 tgtttgccaa agcaaagctc aatatcgttt gcatcataac agccgttaca gctgatactt
    48301 tctggaagtc acttacgttg attttgattt ttttgttttc aaacatctct ttctctctct
    48361 ttttctttat taccaaagtt ctttggttaa tttaggtcct tcgactgttt tcaatagaat
    48421 ttttttctct ggctctctca aatactcttt tagtgaaaaa ccgccttgcc gagtcactac
    48481 ttgggtatcg aattgctcaa aaaaagcagg atgcgattga attacctgaa ttggcaattc
    48541 aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg gtttctttta ctgccggaat
    48601 cccagtcgat aattcaaatc ctaagctgcc gccgattccc catttacatc ctgataatat
    48661 gtttctagca gcatgaaatt gctgataaac aggataatcc atgattttct cttgtctata
    48721 cgattcttgt tcgatgatgt cccacggatg aatgacttgt ttgacggcat gttttgagat
    48781 ttctgttgac catctctgat tgcgggcagg tccacgtaaa ccaatcggta gtatttctgc
    48841 gtgatccgtc gacgcacgac gaactgttcc gaacatcttt tcagttagaa cgacccattc
    48901 aggcaaggtg aacccttcta tttcatgttt atcaaaaagg acaatatcat gtgcttgaat
    48961 cattttttat gcatcccatt cttcgtccat tttggcacgg actttatttg tttcgacacg
    49021 tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc agcatcgtgt catttgtatt
    49081 gcggacatct tgaatggctt cattgattgc ttcgatgact ttgctgacat tttgttctga
    49141 agcagcttgg tcggtgatat cttctaggaa atgatagatc gctcctagtt ttgtataatt
    49201 attgatatcg taagcgatag ctggaacttt ttcagcagct gcttcgattt cagcaaccga
    49261 acgttttgtc acacgtgcag cactttcttt agacatcgct tggacggtaa tttctttatt
    49321 gtttaaagca atcaagcggc ttgattgtaa tccatgtgct aaaaatgcac ctgaaatagc
    49381 atttcctgca atcaatccaa taactggatg tccagcttga cggagttttg cataagcagc
    49441 agcactatta gccaaagcca catggatacc gatcaattct tcgttatagc cgtatgcttg
    49501 gcttggtaca tcgatcacaa cgatgattgg ccgtttcact tttttgtctt tatcttcttc
    49561 gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca actaaaccaa cttcaccgtt
    49621 tcgtacacgg tacatcgggt ttttaggatc aggtacgatt gatgtcacga tggcttcttc
    49681 tatgaattta cttgatttac cataatacac agtactaata gaagaagttg gatttttcat
    49741 gccagttagt gccgcaaacc aatcgtaacc aacacttgct gcagctggtt catttccttc
    49801 tgctacttct ggtaagtcaa ttgattttgc ttgatggtta gcaattgctt cattgtaatc
    49861 gtcaatggcc attggttttg aaagatctaa agaatcaagt aatgataaat aaaatgctga
    49921 gctttcactg cgatgcgtgt cttttcgttc gtccattgct gcgatgacag cagctttgat
    49981 tgcttctaca ctatcagtca ctaattcatc aatgattttt gattctaccc gttgacgaga
    50041 tcctaatgta ttccaaatca agtctttgtc actagaatca aattctcgaa cgcctgcttc
    50101 ttgttcaata acttctggtc cattcaaacc gactctcgct ttttttgttg cgattaaata
    50161 actcattaat gctgaagtaa tcgacatacc gccaaatgat cctacacgtc ctggaatcaa
    50221 accgatgact ggtacatatt gttttagagc aatgattaca tttccgattt ctgaaataga
    50281 taacagtccg tagtttgctt cttgcaaacg aacaccacct gtatctagta caatgactgg
    50341 aaagatctca tttccttttt tattttcttc taacacatgt tcaagtgcag caacgatttt
    50401 tgcaccggaa acttcaccga taccgccacc ttggaatttt ccttccatag cgattactac
    50461 tgcttgtttg ccattgatcg tgccacggct gacaacgatc ccgtcatcac tttcagggac
    50521 gattccttgt gctggtaaac ttggcgcgat gatttgatcg aatggatcaa tcaattcacg
    50581 tccattgtca agtagagcgt tcactcgttc tctggcgttt aattctacaa aactattttt
    50641 catctgaaag ttcctccatt gcttgcgtca atcgtaggta cacaactcct ggtgtagcac
    50701 cgaagtcatt gatcacgata tctgctgaaa ttggataacg atcaaaaaat ctcgttaata
    50761 cattgttcca tacttcgccg aagccatcgc ttcctgtaca aatttttaca gtagattctg
    50821 ttttttctgt tggaaacatc aagatttcca aatctcctga accgaccact cctacatgga
    50881 ttttcttatt gatcggctgt ttcgttggat atttataagc taattgttcc atggatctct
    50941 cccctttttg taaaacagag catacacgtt ctgcttattc gtctttcttc ttccatctgg
    51001 gtaaatggcg acaaaaggtt tttctcgcca attttatgac caagttctga atttcattgg
    51061 tggttcatac aggccatctg accattcgat caaatcatcg atggtttgtg cagctaataa
    51121 tgaacgtttc gcttcactac gtttgatgcc tagatcttct ggtaatttca cgatgccggc
    51181 tgcacgcaag ctatctagtg attctttcgt gctagtcatt ccaagaggtg taacaccgcc
    51241 aattgctgca attgctgctt gtcgttcttc ttttgttttt gctttgtaca aataagcaat
    51301 tccttcttct gtcacgatat gtgtagtatc ttcgctataa atcatcactg gtgcattggc
    51361 taagcctgct tgtttttgaa cagctagtgc atctaattct tcgacaaaga ctggtttttt
    51421 atttgaacca tatgtttcaa ccatttgaac aactaatttt tggcctttcc ctaatggatc
    51481 atcattgtcg cgaagtgatt gccatgctgg tgtagaatga cgacgaccgc caggattatg
    51541 tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct tttgttacgg tagaagagtt
    51601 tccttcgtaa tctaattgca aggttgaacc gataaacata tcgatggcat attgtcctgc
    51661 agcttgtgat aatgttcggt tactgcgcat cgtgccgtct ttaccgacaa aaaagacatc
    51721 tgggcgagca gcaatatatt tttccatacc aacttcgcca ccaaaactat ggatgctttc
    51781 aacccaacct gattcgatgg ctggaatcat cgttggatgt gggttcaata cccagttctt
    51841 cgcgattttc ccttttaacc ctagtgattc accataagtt ggcagtaaca attcgatagc
    51901 tgctgtattg aatccaatac cgtgattcaa agattgtacg ccgtgttctg cgtagattcc
    51961 tttgattgcc atcattgcca ttagaatttg caattctgtg atgttttgag gatctcgtgt
    52021 gaataaagct tctaattgat aaggtttatc tgctttcacg acaacgtcta cccagtcacc
    52081 agggatatct acacgtggta aagaatcgac aacttcgtta acttgaacga tcacgatacc
    52141 atctttgaaa gctgcagctt cgatgattgt tggtgtttct tccgtattga aacctgtgta
    52201 caagttgccg tctttatctg ctttatctgc agctactaaa acaacatttg gaatcaaatc
    52261 gatgaataag cgtccataaa gttccaaata agtatggata tcacctaatt tcattgtgcc
    52321 gtcttcaatc atttgggcga cacgcaagct ttgtgcacca gcataagaga aatcgatctt
    52381 gcttgcgatt cctttttcaa aaatattcaa atgttctggt cgtgagatac tagacatgat
    52441 caaatgcaga tcattcactt tttctgcatc tactttttca agactttctg aaagaaaact
    52501 tgcttgtttt tgattgtcgc cttctaatac aactttgtcg ccgggagcaa tcagtttttc
    52561 cattacttct acgatttttt cagtttctac atacttgcca ttgagcaagt tgctgatttt
    52621 ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag ttgcggtttg gtttttccat
    52681 aattagtcat ctccattttt gatatagaaa cgtggcgtca tcaataccat gttggtctag
    52741 attgatcgta cggatccctt tatctacacc aaattttgta tgaatcagct ttgtcaaagt
    52801 attgccgggg ggaaattcaa tcgtcagatc cattcctaat tcttttgcca agtccatcat
    52861 ttggttccac tgcacaggga aagctaggtt ttccaacaaa tctttacgaa tctcttctgc
    52921 gtcctgagtg gctcttccat ccgtattttt tatatacaaa catctagcat cagatagatg
    52981 gacagactgc atatacggct tgaatttctt gaccgtatca ttcatcaaga gacagtgaga
    53041 tggcacaggg actttcagca gcttcgctgt ttgcgcttgg tgccgtttgg cttctgccaa
    53101 cgtaacggat aaaccttcga tactcccaga taacgtatgt tgcatcggac aattttcatt
    53161 tgacagataa actggatggt caggatcaaa tgtttttgct acaatttcct cagcttcgct
    53221 tctcgtcaca cctactacta cgcccattgc ataaccgctt ggataagctt ctttcattaa
    53281 gactgcgcgg tgatagacta gttccgctgc attctcaaat ctcaaagatc gacaagcaac
    53341 agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata tcaggctgaa taccgttttt
    53401 ttccatttca tctaaataaa atactgcttt tgtcagtaat gctagctgga taaaaaccgt
    53461 atcttgatag ttttcttctt tatcaactaa ccgatacccc gtcacatttt ctaaacgttc
    53521 catgtacatt tcagggatat ccgctaacat ccctaacttc tgactgcctt gtccaggaaa
    53581 taagaaacct gtactcatga gcctcgcctc ctcagcactt actttttcca ctgctatctt
    53641 agtcaatttc tgctataata aaaaatgcca aaaaaagaaa aaaccataac aaaaaggtat
    53701 ggatgggatg gataattatg aaattgcttc atctgcagta ttttaaaaaa gtattcgaaa
    53761 caaaaaatgt tacccaagca gccaaagaat tatttatttc tcaacctgcc ttgagccgtg
    53821 cgatcaaaca tttggaaaac gaattaggtg tccctttgtt ttaccatagc ggcagaaaca
    53881 ttgagacgac cgtttatgca gaagaatttt atccatatgc agtcaagaca ttagacacat
    53941 tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa gatcgttact tccgtcatat
    54001 tgtatttaga ggtagcctct gtttctattc cgaacttggt ccgaattttt atggaaaagc
    54061 atcctgatat ccaattaacg atcatgcaac atcatttacc agatgatatt accgatcctg
    54121 tactgtacat cacttcggag atgaagcctg gcatgaccaa tattcctatc atgaaagaac
    54181 ctgtttatgt cgccattcct aagaaacatc ctcttgctaa aaaagaacat ctgcaattag
    54241 aagatatcca acgcaccccc atcttgatgc tgtcaaagag caacgccttc cgtcatacac
    54301 tggatgcagc acttgaagcg aaaagtatcg aattgacgat cagttcgacg acagatgatc
    54361 ctgcgacatt acgttctatt gtcaagcaag gattagctgt tagctttttc ccaaaagttt
    54421 cttggtctta cgataaaaat gatcctttcg tcttgcggga aatcaaagat ttgcccttga
    54481 cacgaacaat ttatttatct tctagcttca cagaagatca tcctttgatc aaaatgattg
    54541 caaaaacgat gcgggaattt tatttaggaa aataaaaaaa aaagagctgg gacaaaattc
    54601 ttgtcgcagc tcttttttcg aacaaacggt gttccagaag taatttctta gaaaataagc
    54661 cgaaatttcc caaaaatttg agaagcaatt ttcgtaa
//
LOCUS       DAZUMT010000004        51536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000004 DAZUMT010000000
VERSION     DAZUMT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.004"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..268
                     /locus_tag="ACG8FM_000215"
     CDS             95..268
                     /locus_tag="ACG8FM_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092910.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            complement(334..942)
                     /locus_tag="ACG8FM_000216"
     CDS             complement(334..942)
                     /locus_tag="ACG8FM_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS5092911.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(948..1976)
                     /gene="vanB"
                     /locus_tag="ACG8FM_000217"
     CDS             complement(948..1976)
                     /gene="vanB"
                     /locus_tag="ACG8FM_000217"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS5092912.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            complement(1969..2940)
                     /locus_tag="ACG8FM_000218"
     CDS             complement(1969..2940)
                     /locus_tag="ACG8FM_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS5092913.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(2937..3764)
                     /gene="vanW-B"
                     /locus_tag="ACG8FM_000219"
     CDS             complement(2937..3764)
                     /gene="vanW-B"
                     /locus_tag="ACG8FM_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS5092914.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(3782..4588)
                     /gene="vanY"
                     /locus_tag="ACG8FM_000220"
     CDS             complement(3782..4588)
                     /gene="vanY"
                     /locus_tag="ACG8FM_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5092915.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            complement(4759..6102)
                     /gene="vanS-B"
                     /locus_tag="ACG8FM_000221"
     CDS             complement(4759..6102)
                     /gene="vanS-B"
                     /locus_tag="ACG8FM_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS5092916.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(6105..6764)
                     /gene="vanR-B"
                     /locus_tag="ACG8FM_000222"
     CDS             complement(6105..6764)
                     /gene="vanR-B"
                     /locus_tag="ACG8FM_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS5092917.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(7382..7549)
                     /locus_tag="ACG8FM_000223"
     CDS             complement(7382..7549)
                     /locus_tag="ACG8FM_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092918.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(7707..7892)
                     /locus_tag="ACG8FM_000224"
     CDS             complement(7707..7892)
                     /locus_tag="ACG8FM_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5092919.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            8037..8408
                     /locus_tag="ACG8FM_000225"
     CDS             8037..8408
                     /locus_tag="ACG8FM_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5092920.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            8666..8995
                     /gene="mobC"
                     /locus_tag="ACG8FM_000226"
     CDS             8666..8995
                     /gene="mobC"
                     /locus_tag="ACG8FM_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS5092921.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            8956..10284
                     /locus_tag="ACG8FM_000227"
     CDS             8956..10284
                     /locus_tag="ACG8FM_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5092922.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            10281..10652
                     /locus_tag="ACG8FM_000228"
     CDS             10281..10652
                     /locus_tag="ACG8FM_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS5092923.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            10835..11071
                     /locus_tag="ACG8FM_000229"
     CDS             10835..11071
                     /locus_tag="ACG8FM_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5092924.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(11371..11574)
                     /locus_tag="ACG8FM_000230"
     CDS             complement(11371..11574)
                     /locus_tag="ACG8FM_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS5092925.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(11578..12522)
                     /locus_tag="ACG8FM_000231"
     CDS             complement(11578..12522)
                     /locus_tag="ACG8FM_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092926.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(12523..16443)
                     /locus_tag="ACG8FM_000232"
     CDS             complement(12523..16443)
                     /locus_tag="ACG8FM_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS5092927.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(16592..18673)
                     /locus_tag="ACG8FM_000233"
     CDS             complement(16592..18673)
                     /locus_tag="ACG8FM_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS5092928.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(18670..19899)
                     /locus_tag="ACG8FM_000234"
     CDS             complement(18670..19899)
                     /locus_tag="ACG8FM_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFS5092929.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(19889..20140)
                     /locus_tag="ACG8FM_000235"
     CDS             complement(19889..20140)
                     /locus_tag="ACG8FM_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFS5092930.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(20163..22154)
                     /locus_tag="ACG8FM_000236"
     CDS             complement(20163..22154)
                     /locus_tag="ACG8FM_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS5092931.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(22186..24585)
                     /locus_tag="ACG8FM_000237"
     CDS             complement(22186..24585)
                     /locus_tag="ACG8FM_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFS5092932.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(24515..24937)
                     /locus_tag="ACG8FM_000238"
     CDS             complement(24515..24937)
                     /locus_tag="ACG8FM_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFS5092933.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(24951..25487)
                     /locus_tag="ACG8FM_000239"
     CDS             complement(24951..25487)
                     /locus_tag="ACG8FM_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFS5092934.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(25524..26387)
                     /locus_tag="ACG8FM_000240"
     CDS             complement(25524..26387)
                     /locus_tag="ACG8FM_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFS5092935.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(26479..26694)
                     /locus_tag="ACG8FM_000241"
     CDS             complement(26479..26694)
                     /locus_tag="ACG8FM_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFS5092936.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(26771..28579)
                     /locus_tag="ACG8FM_000242"
     CDS             complement(26771..28579)
                     /locus_tag="ACG8FM_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS5092937.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(28576..29052)
                     /locus_tag="ACG8FM_000243"
     CDS             complement(28576..29052)
                     /locus_tag="ACG8FM_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFS5092938.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(29127..29339)
                     /locus_tag="ACG8FM_000244"
     CDS             complement(29127..29339)
                     /locus_tag="ACG8FM_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5092939.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(29361..30326)
                     /locus_tag="ACG8FM_000245"
     CDS             complement(29361..30326)
                     /locus_tag="ACG8FM_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFS5092940.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            complement(30817..31338)
                     /locus_tag="ACG8FM_000246"
                     /pseudo
     CDS             complement(30817..31338)
                     /locus_tag="ACG8FM_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(31354..32052)
                     /locus_tag="ACG8FM_000247"
     CDS             complement(31354..32052)
                     /locus_tag="ACG8FM_000247"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5092941.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(32009..33670)
                     /locus_tag="ACG8FM_000248"
     CDS             complement(32009..33670)
                     /locus_tag="ACG8FM_000248"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS5092942.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            33888..35288
                     /locus_tag="ACG8FM_000249"
     CDS             33888..35288
                     /locus_tag="ACG8FM_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS5092943.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(35540..36619)
                     /locus_tag="ACG8FM_000250"
     CDS             complement(35540..36619)
                     /locus_tag="ACG8FM_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5092944.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(36705..37664)
                     /locus_tag="ACG8FM_000251"
     CDS             complement(36705..37664)
                     /locus_tag="ACG8FM_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5092945.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(37676..39187)
                     /locus_tag="ACG8FM_000252"
     CDS             complement(37676..39187)
                     /locus_tag="ACG8FM_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS5092946.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(39209..39772)
                     /locus_tag="ACG8FM_000253"
     CDS             complement(39209..39772)
                     /locus_tag="ACG8FM_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5092947.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(39778..40641)
                     /locus_tag="ACG8FM_000254"
     CDS             complement(39778..40641)
                     /locus_tag="ACG8FM_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5092948.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(40655..41551)
                     /locus_tag="ACG8FM_000255"
     CDS             complement(40655..41551)
                     /locus_tag="ACG8FM_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5092949.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(41726..43015)
                     /locus_tag="ACG8FM_000256"
     CDS             complement(41726..43015)
                     /locus_tag="ACG8FM_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5092950.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(42987..44504)
                     /locus_tag="ACG8FM_000257"
     CDS             complement(42987..44504)
                     /locus_tag="ACG8FM_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS5092951.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            44702..45592
                     /locus_tag="ACG8FM_000258"
     CDS             44702..45592
                     /locus_tag="ACG8FM_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS5092952.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(45665..46489)
                     /gene="nadE"
                     /locus_tag="ACG8FM_000259"
     CDS             complement(45665..46489)
                     /gene="nadE"
                     /locus_tag="ACG8FM_000259"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS5092953.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(46501..47973)
                     /locus_tag="ACG8FM_000260"
     CDS             complement(46501..47973)
                     /locus_tag="ACG8FM_000260"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS5092954.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(48610..48972)
                     /locus_tag="ACG8FM_000261"
     CDS             complement(48610..48972)
                     /locus_tag="ACG8FM_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5092955.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            49195..49947
                     /locus_tag="ACG8FM_000262"
     CDS             49195..49947
                     /locus_tag="ACG8FM_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5092956.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(50087..51010)
                     /locus_tag="ACG8FM_000263"
     CDS             complement(50087..51010)
                     /locus_tag="ACG8FM_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5092957.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(51025..51297)
                     /locus_tag="ACG8FM_000264"
     CDS             complement(51025..51297)
                     /locus_tag="ACG8FM_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5092958.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        12835 a        12717 c        11439 g        14545 t
ORIGIN      
        1 gaggggcaag gctttttttg agtggtggaa gtggattaga gcatggcaat agcaaaacag
       61 catagcggca tcctcctatg ggccgccaca ccttttgctg ccagaaaaga acggcggcct
      121 tgatttctca aaaccgccgc cagcaaaata caaaatgggc gtgggaaagg tctgcccaac
      181 agcggttttt ttcttttctg ccgctgggca gattgaatac tataatttgc aatgtattac
      241 aaaagatgct ttaaccactt tccagtaaaa gattgtccat gaccagcata gattttatca
      301 aatccaaaat ctgatacaaa accaatactt tcattatgaa acggcaaaat taaaataggt
      361 atcggggtat ggctcatcaa tcaacttata gtgccaccat tcaaagcgat aagactgaaa
      421 tccgctgctt tccatgattt tacgcaagca tcttcgattt tgtgcctctg catcccctat
      481 cccttttgct gtatgatgcg accgttcgtc cataaaatcg aagttacttc ccattgaaac
      541 aagttcccct gtatccaggt ggtagagcgt aagatcaatt gcgcttccac ggctatggct
      601 ggattgtgag gccacataac cctttgtaat caactcggct cgctctatat tggggtaaaa
      661 tttttctttt gtgagattgt cctccggttg cgctgcccaa cgcaggaagc agtccaccgc
      721 agttcttggc cgatatccat cccataaaag caagccatag ccaagcgcag ccgcatggat
      781 ttgtgcctcg cgcagagcaa acgccacggc ctttgtgccg atgatgcgat tcacctcata
      841 cccatccacc ggttttcctg tgaagttatc ccatgtggcg tacttggcat cccaacggac
      901 gccatgcaac atttcatcta aaaacaaaaa accattttcc atacttatca cctctttaac
      961 gccaatgtaa tcaggctgtc aatcagtgca ggaagcgtga ttcctgcggc ggccatcata
     1021 cgtgggtagc ggctgtacga tgtaaaaccg ggcagggtat tgacctcatt tagaacgatg
     1081 ccgccatcct cctgcaaaaa aagatcaaca cgggcaagcc ctctgcatcc aagcacccga
     1141 tatactttct ttgccgtttc ctgcacccga tttcgttcct cgaccggaat gtctgcggga
     1201 actgtaatca tcgcattttc tgagcctttt tccggctcgt tttcctgatg gatgcggaag
     1261 gtaccgtggc tcagccggat ttgatccact tcgccgacaa tcaaatcatc ctcgttcccc
     1321 atgaccgcac acccgacctc acagcccgaa atcgcttgct caattaagat ttttccatca
     1381 tattgtcctg ccgcttctat cgcagcgtta agttcttccg taccgtttac tttggttacg
     1441 ccaaaggacg aacctgaccg tgccggcttc acaaagacag ggtaggtaag cgcacccgcc
     1501 tccggcttgt cacctttatc aatcatttga aattcgggaa cggcgatgcc cgcatttttt
     1561 gtaagaatgt aggccagtga tttgtccatg caagctgcgg agctttgaat atcacagccc
     1621 acatagggga taccagacaa tacaaacagc ccctgtatcg caccatcctc cccgcatttg
     1681 ccatgcaaaa ccgggaaagc cacatcaata cgccgtgttt cgtattcgct ttctttcatg
     1741 acaagcagcc catgcgtttt cctatccggg gagagtatgg cggggagact gtcggcttcc
     1801 cattccgtac atggcttctt gcatagcttc catacaccgt tttttgtaat tccgatgtag
     1861 tgcggatcga atttttccgt atcaatgttc gcagcaattt ctattgcgga ttttaccgac
     1921 acatcatgtt cctccgagca accgccgaag atgattgcga cttttattct attcatgctg
     1981 taaactcctt tcaaaattga gacaattcct gattgttttt tctgtagtat cctgcagcac
     2041 acgctcagtg tagtacgccg tatggggtgt gatgatcaca ttcggcatcc ttaaaagctg
     2101 cgacaaaaag ggatggtcaa gcactttctg cgagcaatcg gtataaacaa actgatcctc
     2161 gccctccaac acatccagtg ccgcaccacc cagctttccg ctttccaacg cctccaccag
     2221 cgccccggta tcgacaagcg ctccgcgccc ggtgttgatt aaaaatgcgc cttgcttcat
     2281 ctctccaatt tgcttctgac cgatcagatg gcgggtatcc gcacaaagcg gcacatggag
     2341 cgtaacaata tcgctgtttt ttagaagctc atcaagctgg acataatctg catccatttt
     2401 tcggctgtta tcataggcca gcacatggca tccaaatccc cgcagccttt tgacgaccgc
     2461 ttgcccgata tggccggttc caataactcc cacagtcata tcccccagtt ctttcccccg
     2521 gatacgatcc aatctgaaat cttgttgcgc cacggcgtgc atggtgggtt ttgcaccccg
     2581 tatggccatc agcatcagca tcaaagcata atccgcaacg ctgtccggcg aatacgcaac
     2641 cgtgccaacc gagattccca ttctctcggc ggcagtcgta tcaatgtgat cgcagccgat
     2701 gctgcgggta gaaatgtatt ttaccccgac ctttctcagc gcaagaattg tcgcctcgga
     2761 aacctcggac ttatggccta cgctaacgca ttgattgcca gcggccaatt ttgcgttgtc
     2821 tgccgatatc gcatcgctaa tcagcgtagg gataatatga aaatccggcg ataaagcgcg
     2881 gaaagcattt gcctcatcct gttcgcatcc aaaaacagta atgcccacac tttttctcat
     2941 tggttctcct cctgaatatc cacactttcc gggaggggat aacagatttt gcatttgttt
     3001 gtataaagca gcttgcaatc tattatttcc ccggtaccta aggcaacttg catccgttta
     3061 acctgggcat attcataaat cccgccactt tcacggacat actggatact gccatttgta
     3121 attttgtata atgcttgggg ctctttgtcg gcggacacct gaccgatgat tttctcatca
     3181 tctaaggtca cccatatttg gtaggtgcag tcggtatcgt ttcgcacttt taaatcaatc
     3241 cagccctctg aaatggttgc atccacccct ttaatctcgt cactgtttgg ctctggaaac
     3301 tcctttactt cgtgacctct gcgctggata attgtcaatg gcgtatgcag gaacatccaa
     3361 aatagtaaat tgctcatctg gcacataccg ccgcccgaca tggtggtgag tttaccattg
     3421 gtcaccgtaa ggccgtcttt atagggggta tctttgtccg catggcgtac cagccaccag
     3481 aaagaaaagg gttcccccgg tcttatcaat aagccgttta aggtctttgc tgccagcttt
     3541 aagttgaaca ccttgttttc ctggtatatc atgtcaaatc cggtattgtg gttgtataat
     3601 gcgcaatccg tttcaaagag caaatgggaa agcgattttt cccctatcgt ctgcgcatag
     3661 cgacagccgt caaatctcat tcccgcataa aagcacattt ttctctgcgc ccgtctcatt
     3721 ggaagcagga acgggaaacg ctgtgtcaat ctttttctgt ccataacata acatcctttt
     3781 ctcatttttc tggttttaaa tattcctcaa ggcaaacccc ttggttgtac atctctgtgg
     3841 cagcttcgat gccaacatat cggtaatgcc acggctcgtt gctcacaccg gttatctctg
     3901 tcttgtctgg tgggtagcga cgaataaaac caaaacgata gctgttttca tccagccatc
     3961 tataaacctc gttgcctgtt gaatgaattc cgtccgcatt gatatccaca gcaagaccaa
     4021 gctgatgctc gctcgttccc ggcacggcca cccaagtttc tgcttccgct ttagcctgtg
     4081 cagaggtgta gcctttcgcc ttatattcgg caattttttc atccataatt tcttgctgtt
     4141 tttctgttgt tcggtatccg gatgcgacaa tcgggtaaac tccatcagtt cttgcggcat
     4201 caaacaaatc ttgaagatag ggagaaatcc gaatatctat ccgctcacca tttgatagtt
     4261 gctcaagctc cacatcgtac tgtgcgggga tggggttctg cctgttcact aaaatcaggc
     4321 tccattcctg ttcatctccc tgttctttgg cggtggcaag agcgttttcc tccaaattct
     4381 ccactacgtc agtgctgctg cctgtatgag ttgcagggat gggcggtatc tgtgttgcct
     4441 ccaatacgga agccagtctc cgccccaaaa acagcgtgca gactgtgaac gaaataatga
     4501 acgcacatag aaaagcccgc ttttccacgg attgtttcat atgccgtttg tgatgatccg
     4561 cattggaatg atagttgctt ttttccattt gatcgaatgc ctcctttgca ataaaactac
     4621 gatttgtggc tggtacgatt gttattatgg caagcgcatc tgtactttcc ctgtagccag
     4681 tctgtagttt tcctgtagca gcaaaaaaag accggcaggg ttatacctgt cggtcaaaat
     4741 catttaagtt ttaaatattt acaatgttga tgtgagcggt aaatccagcc agaacaaaac
     4801 gccatccgag gtgttttcca gcgcatactg gaggctcatg gcatccagcg ttttttgtac
     4861 gatggcaagt cctaaaccgc ttcgcccact ttttctgctt tgcgcctgat caatgcgata
     4921 gaatggggtg aacagccttg gaagcgcagt atcatcaatg tgaacgccca tgttcaaaac
     4981 aaaaaggcgg catttttcag caccaggctc actccatatc cgtacctcgc ctccctgcgg
     5041 cgtgttctga actgcattca atatgacatt ggatagtgcc ttttggagca gcctcggatc
     5101 ggacaggaca atttgcccgg ctggaatatc tgtgacgaac cgctggttgt ttgcctctgc
     5161 caaggtttga aaatcgggca gcaactcggc aaccgtgcgc cctatgtcca acggttcagc
     5221 tatgggtacg attctcccat cattcaggct gacaagctcc agtatttcgg aaatgatttt
     5281 gccctgccta tccatcattt tgatgcattc gcgcagatac ttagaatgat ctttgtagtc
     5341 accgatattt tcaagcattc cctccaacag aacgcttgta gccgcgatgg gcgtttttag
     5401 ctcatgagaa gctgccgcaa agaaatatcg ctgtgtttcc tccaactcat gttcccttgc
     5461 gatttcatcc tccagccttg cgatggtttc tttcagcctg acatacatgg aatgcatatc
     5521 atgagccagt gcgccaagct catcctttcg ctccagcggc ggcggtacat ctttcaggtt
     5581 tgccatttta ttcgcactgt ccgctaaggc tttgatcggc gttgtcattt gccgcgcaaa
     5641 gatatacgcg cataaaaggc tgaatacaac cattatcgca atcataacaa ttccccgaat
     5701 tgtcagccct tgataaagca atcccacacc tgccttgctt tgagcaacaa tcgaaatatt
     5761 atcatctcta tgtaccacat aaagaaagtc gggtctaaaa ctatttgatg tattggcatt
     5821 tggtgtggca tagagtacgc ttccctcttt atcttcaata taaaactcaa aggattggtt
     5881 attgtagtga aacagccctg ccacctcttg aatatcaagc ctatcgctat tctgaatcag
     5941 ttccaccaat ggctgatagg attttactgt ttgctgtaat tccatcactc tgaaataaga
     6001 cacaaattgc tgtgcaaaca gtgttgctgt tacaccgaca agcagtagca gggcaatgat
     6061 cgtataggaa aaaaccttaa tgaaaatccc ttttctttcc attattatga ttcctccaat
     6121 cggtatccta caccgcgaat ggttttgatg atattttccg gcagcttggc ccgcaaattt
     6181 ttgatatgag tgtggactgt gctgccatca ccctcaaagt catatcccca tatacgtgac
     6241 aaaatgattt catgcgttaa ggttctgcct ttgttctgca ccagcagcaa aaggatttca
     6301 aattcttttc gtgtcagggg cagctctgta ccgtcacaaa gtaccgtgaa atcctccggc
     6361 agaagtgtca gcctgccgac acggatttcc tttgccagcg caccgctgcg ccgcagcagg
     6421 gcttccaccc gctttagtaa aatctgcatc ttgaatggct ttgttacata gtcgtctgcc
     6481 tctgcatcaa acgcccggat ttggttttcg tcatccgaca gggctgtcat catcagaatg
     6541 ggagtatcat ttttcgcacg aaattcacgc aaaagttcat gcccgttcat accgggcagc
     6601 ataatatcaa gaataaccag ttgataagtg ttttcgtaaa acttggtgta tgcctcattt
     6661 ccatctgtgc aggcatccac ctgatatcct gcctcagcca gaaacgccct tactgtatta
     6721 cagatatgat catcatcctc gacaagtaga attcgtatcg acataaaaat ccctccattt
     6781 taagaagata acataacagt ctgtagtttc cctgtagttt caaagcgatt tcttttcgga
     6841 ttttaatcat ccgtcattgg gatactcgct tcttttcatg gtgtatgtat ctatattatt
     6901 ggtttaaacg aattaaatca agataataat aattaacacc ttcttaatgt ttgggatgtt
     6961 tcgattacag cggttgtcaa aacatcccga taatactggc aagcaatgga tcagaatatc
     7021 ctttacggat attctgatcc accacttgcc cgcctgcggc gttctcgttt tgcttgttgg
     7081 ggacacccca agcctcagca aaatgcgcgg cctctttttt gcgaatgcac cttgcggtat
     7141 ggcttttcac acaggcaggg cctgtgtgta ctccctccgg tcgctcttgc cgcactccgc
     7201 gcagcagatt cagtcctaat accgttcaag acagtagaaa atcagcaagt ctatattcct
     7261 catatcttca tcatccgtgt ccttgcagtt acaggaaatg taataatcgc caaatgtaaa
     7321 tcccagaata ttttcctcaa aaagccgtac ctccccggta ttaaaatcaa tcaccccata
     7381 ctcaaaggcg cattagacca gcagttatcc aatgtctgga gacttcacca taaagaatcc
     7441 agatgccggc cttttcaatt ctaccatata cggctgatac agcagcagct tcctcgtctt
     7501 tgtttcgggc attttccatt tctgtcatga taaagtttcg cttcttcata catccatgtg
     7561 taaatctttt ctttctgttt cagatgagaa tactttttat cggtctggag cagcctgcca
     7621 tctatcatct tatagttttt ccgtgacaaa ataccatgct ttctctctct tcaaaaccat
     7681 atccaaaatg cgccggtttc attatactat tcagtaaccg cctttccttt ctttgtacgc
     7741 ctgtcaatcg gcttttccgc agttttggga atgagccaga tcgtcgccat ctttacagcg
     7801 ccggggatgc gtcccgcagc acaatagtag tttatccatc gtgggggcac accccatttc
     7861 tcgcctgcct ctttcaacgt catgtagtcc atagtacacc tccacagggt acattataat
     7921 tcttttgctc gaagaataaa agaaacaaaa tgtgaaatat aaactttaag aaaggtgcat
     7981 ataccaacat tccacgggca gagtatgaaa tatacaatgt aagtgggagg tgcgatatgg
     8041 cgaatattga agattgtccc ggctttgaaa ctttcggcgc ggatgtgaaa gcggcccggc
     8101 aagcaaagcg cgtttcgcgc aaagcaatgg cagagaaaat aaatattgac tggcgctatc
     8161 ttgccaacat cgaaaacgac ggggccattc ccagtctgcc ggttgtcata cagcttatta
     8221 aagcctgcgg gcttcctgtg gaacgctact ttaacccgga gatcatgcgg gaggaaagcg
     8281 aactgcggca aagggtcagc cacaagctga aactctgccc cgaagaatac ctgccgatca
     8341 tcgagggcgc gattgacagc gccatactaa ttgaaaagcc gaagaagaat gaaacggagg
     8401 atgtgtaact ggcatcctct gttttacttt tagcgggagc tgcacagcgt agcttctatt
     8461 tttttgcact tttttctttc cagagggcta ttttgcgggt agtttggggc tttggaaacg
     8521 tcttgttagt ggagaaacag taacgtagca agcataggga gtgtagccac tcccggaaaa
     8581 acggcctgcc ccgtccctgc cgggccgcgt ccgttttgct tgttggggaa atccccaaac
     8641 ccctgaatgg aaaggacggt gaagcatggc aaacagaaag cgaaaaatcg tgctgcgcgt
     8701 ccctgtgacg ccggaggaac gggctatgat tgaacagaaa atggcgctgc tgcatacgaa
     8761 gaacttttca gcctacgccc ggaagatgct cattgacggc tatatcgtca acatggacac
     8821 cagcgacatc cgggcgcaga ccgccgagat acagaagatc ggcgtcaacg tcaatcagat
     8881 tgcaaagagg ctgaacggta tggggccggt gtacgcacag gacatcgagg atataaaggg
     8941 ggcgctggcg cagatatggc agttacaaag atacatccta tcaagtcaac gctgaaaaaa
     9001 gcccttgact acatcgagaa cccggacaaa actgacggca agctgctggt ggcctccttt
     9061 gcctgctctt atgaaacagc ggacattgaa tttgaaatgc tgctggcgca ggcataccaa
     9121 aagggcaata atctgggcca ccacctgata caatcctttg cccccggcga agccacgccg
     9181 gaacaggccc atgagatcgg gaagcagctt gccgaggaag tgctgcaagg gaaatatccc
     9241 tatgtgctga ctacccatat tgataagggc cacgtccaca atcacattat tttctgtgcc
     9301 gtggatatgg taaatcagcg caagtacatt tccaacaaac agagctatgc ctatatccgg
     9361 cgcaccagcg acaggctgtg caaggaaaac ggattgtccg tggtgaagcc cggccagagt
     9421 aagggcaaga gctatgccga gtgggacgcc cagcggaaag ggacgagctg gaaagcgaag
     9481 ctcaaagcag ccattgacgc cgctatcccg caggcaaaag attttgatga tttcctgcgg
     9541 ctcatgcagg cgcaaggcta tgagatcaag ccgggaaagt ttatttcttt ccgcgctccc
     9601 ggacaggaac gctttacccg ctgcaagaca ttgggggaag cctacaccga ggaagccatc
     9661 aaagagcgta tcaaaggccg cgttatcacc agagcgccca aagagcgcaa ggggatttct
     9721 ttgcggattg atctggaaaa caacattaag gcccagcagt cggcaggcta tgagaggtgg
     9781 gcgaagctcc acaatttgaa gcaggccgca aagactttga atttcctgac tgaacatggg
     9841 atagacacct acccggattt ggaaagcaaa gtggccgaga tcaccgccgc cagcgacgag
     9901 gccgccgcta ccctcaaagc tatggaacac cggcttgcgg atatggcggt gctgataaag
     9961 aatatttcta cctacaagca gctctggccc gtggctatgg aataccgcaa tgcagcggat
    10021 aaggcaaaat tccggcgtga gcatgaaagc acgttgattt tgtatgaagc tgcggcaaag
    10081 gccctaaagg gacagggtgt gaaaaagctc cccgatctgt acgccctgaa agccgagtac
    10141 aagcgattgg ccgaggaaaa ggaacggctg tatgagaagt acggcgaagc gaaaaagcag
    10201 atgcaggaat acggcatcat caagcagaat gtggacggca ttttgcgtat gacgccggga
    10261 aaggaatgga cgcaagagct atgagtattc cacgaatgaa ttaccctcaa tatcgcaagg
    10321 cccggaagct gacgcatgag tgctgcaact attgtgacgg caactgcctg ttgctggacg
    10381 acggcgaaga atgtatttgc gtacagagca tttcctattc gcttctgtgc cgctggttta
    10441 aggccgctgt cctcccgctg gatgcggccc tgtatgccga gatcaccaaa gaccgggacg
    10501 aagtgaaacg ctgctgtgag tgcggggcca cgttcacgcc gaagtctaac cgggccaaat
    10561 actgcgcggc ctgctccaaa cggatgcgcc gccgcaaaga ggccgagcgc cagcggaaaa
    10621 gatactggaa acctacgcac ttagagctgt aaaagccctt atatttcaag gcttttccgg
    10681 catggtcagc ggggtatgtg ggtgttttat cctatccccg ccgaagtgct gttctaaatg
    10741 cgtagaattt accagcagaa aagcgccgtt ttctctggtt ccatgcttcc gaatatggta
    10801 tcatagaaaa aatcagtctg acgggggtat caggatggac ggacaagaaa agctgctgga
    10861 ctatgaaacg atcaaagccg ccgttgccgg tgagaggtgg gccacggaaa aagtgttggc
    10921 ccattatgct gactacatca acgagctttc cacggtggag atcagacagg caaacggcaa
    10981 aaagaagaaa gtcattgacg aagatatacg gcagcgcatt tccctgaaac tgctggaagc
    11041 attgccgagc ttcccgctgg aacagagata gacggcaaga gggctgcacc ctcttacgcc
    11101 gctttgcggc tatcacctgc acacccctga aacagataca cccgctgcgc ggctatatct
    11161 gtttcagggg tctaattata gctgcaacct acgcatttaa gggattgaaa accgcccgct
    11221 gcaaggcttc tcccgcatgt ttgcaggggc aagcgaatac ttcatcctat ccccgacaat
    11281 accgggttct aaatgcgtag gaataggcaa aaagaaaaga cgccggacgc aagcgaccac
    11341 atggccgccg cgcccggcgt ctgctgttcg ttattcgtct gtgaagtgga acttctggcc
    11401 gtcatagtcg gcatccgtca tgcgttccag cttggaaagg gtctgctggg cctgcgcccg
    11461 catatcccca tccataacca gcatgacggc ggtgagcctg tttatcgttc tgcgccggtc
    11521 tgcattgtgg tacatacaga tcaggttttc ttcctccacg gtaaagtgca tcattcctta
    11581 tacctccaat tcattctttt tggttctggc cgccgccttt tttgggctgg cggcctgttt
    11641 gctgtcggat agctgcttgc ggatagaggg ccggggcctg cgaatactca catcccggcg
    11701 ctcgtcccgg ttaatcacgg catacacgcc atgcctgcct ttcatcccgg taaaacacag
    11761 ggatttatag ggcagcatgg caaagagccg gtcattatcc ttggtggaag caatcgccgc
    11821 aaagtgcggg gagatctcca ccatgaaatg cgttttattg gggctgttag gcgcggacag
    11881 atctttcagg ctgtccacaa tacgccgggc ctcggtttca atccggctgt catgcaaagc
    11941 ggtgacgtgg actttgaaat cgccggggac ggcccgcccc cggctgtgct gctccgtccg
    12001 tagcgcctcc tgcagcacag ccgggtcatg gggctgggcc tcatagtaca aaaagtttcc
    12061 caatacccgg tcagggcttc cgtcaaaacg ctggctctcc gggatgtcac gggtgccatg
    12121 ctcataaaag agccggttca cactcaccgg ctgggcggtt tcaatgacgt gccggaaatg
    12181 ctgcggatag tccagctcat agagattgcc ccggatagtg ccgccctgtg tgccggtcag
    12241 ctccaccgcg taggccagta tcttgtcacg ggtctgctcc ccgtaaaatt tccatgtgtt
    12301 gtactgtcgg gtgtctttca ggaaaacatc acgttcccgg aaacagtacg tcccggaggg
    12361 ccgggacatc cagagcaggg tcttgtctgc ggcctgatcg ctggcggcat cccgcctgat
    12421 aatgtctttg tcaatgccaa agtcactttg ataaaacgcg gtgttctgcc gcatgatctg
    12481 ttccagcgcg gccagaacgt ccacattttc aaacttctgc atttactcta cctccaatcc
    12541 ctttgatttt tcccgcgccg gtggctgctt ggcctgttcg gtcttggccg cgttgagctg
    12601 cgcccggata gagggctttt ctttctccct gcgggccgca agctgtaagg cgctgggctt
    12661 ttcgccggaa aggggctgga tgcacttgat acggtcagcg acgcggattg tggtgtcgtt
    12721 taactgccgc tgccatgccc cggccttggg cgaccagcgg aaaccgttgc ttttcagctc
    12781 gtcgcgtaca gcagcatccg gcttcccctc aaagaagatt tgcaggcggt tggccacccg
    12841 gttgatctcc acagtgccgc cgtcaaactc ccatcccaca taggccgcct ccttttggcg
    12901 ggtcaattcc tcaatgcggc cctttaaccg gcgtatctcg gcgttattgt tggagagctg
    12961 ccaggactgg aacggttttt tctcatagtg ccagcccgac gccatatccg ctttcaggtt
    13021 ttcaatgcct ctttcggtca gatgggggca tccgtccagc gtcccatgct tgcggtagta
    13081 ggcattgacg gccttcatca actcctgcga cgcttccagc ttttccagtt tcttttccag
    13141 cttggaaacg gcctgcggat cgtcggcgct gataccaccc atgccggtgc tgcggatttt
    13201 gtcaagcaag ccctgaatgt actgaaattc ctccatgttg cggtcactgg cggcgttctg
    13261 cttttctttc gcccgtaccg ggaaattgga cggcccggca atcaggacag aggggacgcg
    13321 ggcggtaatc tcatagccct tattcatgtt ctcggccagc ttgcgggcgt aggtgtcaag
    13381 gaggctgtct atcttttcgt gatactctgg ctccacacgc tttttctggc gctccgcaat
    13441 ttccacggcc tgatcgacat agtgccggta ttccgccgtg gcgctgccgg gccggtagtc
    13501 agaaaagctc atggcttcct tggcgcggcg ggcggctccc tcattgatgg gataatagcg
    13561 gacagccgtt tgcgggggcg cagcctccgg gggttcctcc tgtgtctggc cggaagcaac
    13621 tgccacatcc tcaaaagata gctgttcggg ttcctccggc tgcggttcgg gcggcgtatt
    13681 gttgataatg ccgtcaatca tgttttcgtt ctgctccgta gacagttcgg cggtacgcag
    13741 gggattttcc cgcaaaaact ccggcacttc catatagccg aagctgtcta catagtgggc
    13801 ggtgtcctgt ccattctggt gcagcaccac cacgtcggaa acggaaaggc tgtgtcccct
    13861 aaaatctgcc gggtggtcga tgttgaatgt gcggtaaata tcctccaaag acgtgctttt
    13921 atccagcggt gccgtataaa caaggtcata gttggcactg tccacggcca gccctgcggc
    13981 ctgtaaacgc tcgtatggct caaaccgata gtcgcgggtt tcctccccgc ctcgaagctg
    14041 gtagatggaa aaggtgtcct gttggggtgc ttcatagcca gccgccgcgt attcctccac
    14101 ggtcataccg gctgcggcgg cctcctgcgc gatctcggcc tgtacctgtg ctttgtagtc
    14161 ctgcaagctc tggtcgaagt cagccagttt cggcggctcc ggcaactgat actggccctg
    14221 attgagcatg gggcggcact cctgcacata agagagaagc gcccccaaat aggccgtttc
    14281 ctccggcgtc agggtttccc cggactgtaa gcggccctgc gccgtctgtt ccatttgggc
    14341 aaggctgcaa tgcagcttca tataggggac aaactcgcct aacagcatat cccgatgggc
    14401 cttgtcctgc tcccagccct ctatgccggt tgtagcgatt acatgctttt tccagttttc
    14461 gtcctgcgcg taaaattggt ggtagctctg gatatgttcg atcagggaac cgtccctatc
    14521 tccaaaatcc tgccgcccct cgtagctgtc cggctcgccc tgaaagatac agtcaatgcg
    14581 gaacgcggtt ttgtcatagc ctgcctctgc cggatatgct gcatccagac gggcaaacag
    14641 cgcgtcggcc tcatgcaacg gcatttgctg gccgtccttt agctgggggc tttcgctcca
    14701 tagcacagtc acaagcggct cggctgccgg atcaagggcc ggggcctgtt cttcctcggc
    14761 ctgcgccggg gccgctgcct ccggggtaac gtcaaccgtg acggcctcct gttcctgccg
    14821 ggcctcgcgc tcctgctgaa gctgggcaaa atggccgtcg atctcggtta tcaattcgct
    14881 ggccgtggcc cggatggttt caagggaggc tttcagctcg gataactccc tgccgctgct
    14941 ccaatcggcc acatagccaa aagaatagtc cgacgtgtcc agcccgtaat actggcagac
    15001 ggtataggcc acgctttcgg cctgtacctc gcgggtgctg cggtctggcc gggccgcctg
    15061 ttcctcggcg ggggcctccc ggtcaatggc gtggagcttg gcatgggcaa tctcatggat
    15121 ggcggtcttt agggtctgca attcgctcat gccctcgttg atggcaatgc gctgttttgc
    15181 gtagaagcag cggcccttgg ccccgtctga aatcggctca atgcttatgg gatagggtga
    15241 tgtccgttcc agcgcggcaa agaaatcctg atactgctgc acgtcgccgg taagttcact
    15301 gatatagggc atgggaaaat ccttgccctc ggtctggctg atgtcaaaga ctgtcaccac
    15361 cttaaaggcc ggaaccgtca cttcctgttc ttcggtcacg ggcttgccgt ccgcgccgat
    15421 cacgggctgc tgggtggccg ggtcgatttt ctccatctgc tgcttaacct tgaacggggc
    15481 cggggccagt atcttaatgc ctttctcgcc ccgcttcaca tggcggtcaa attcatcctg
    15541 ccacctgtta tagcccgcca caatcgaacc gccctgcatg gcgatcagca gcgtattgtt
    15601 cagactgtaa tcgtgcatct tggacatagc ggataagtaa gccatatagc ggctactttc
    15661 aaaaagctcc tgtacgccgg tttccagacg gtcagtaatc actttcatgc gttctttgct
    15721 gttcatgccg tccagcatga tcgggataac cgggggcggc gctttgtcgg ggctttcgga
    15781 tgcctccggc tgctgggcct gcggttcctc ccggtcagct tccggctggg ggtagtgcat
    15841 cacccggtat tcctccggca ccggctggcc ctcatggacg cgctgccatt cgtcgccgct
    15901 tctcaccaga tagccgtact gggtaaaaac gccttgttcc tgctgggcaa tgtggcgtcc
    15961 aaactgcgcc gtatcaatcc cggctttcca ttcctccggc atatccacca tgccggaacg
    16021 gttcaggtag tagtcgccca actgtccatc ccccctaacg tcgggaagat gcacatagta
    16081 atccacattt tcggtgtagt cgatgatctg gccgatattc tcaaagccgc cacggggatt
    16141 ttgcagggcg gcgttcagct ctgcgatctc ggcggcgtca agctggccca gccgggcggc
    16201 aaggaaattc agctcgtcca cgtcggcggc cagcaccatg tcatagggaa tggcaaggtg
    16261 tctgtcctcc gggcaggaat acccgttgat aaaaaaatcc tgcggattgt cggcagtaat
    16321 gccaacatcc cgcagggcgg cttgcagcgt ttcggttgtg gtgggcaaat ccagccagac
    16381 gccttgtttg ccctgttcca aaaactgccg gttatggact tggatagaaa atacttcgtt
    16441 cacgcggtca ctcctttctc tgttctgttt caaaaaatag ggggttgggg acttccccaa
    16501 caagcaaaaa tccccggccc ggcagggacg cggccggggg attttaccgg gagtgtagcc
    16561 acactcccta tgcttgctgt gttactgtcc tttagctcct gtgttcaatg cggtagttga
    16621 cgtacttgcc cccggtatca gccaaaagca ccgtcccgtc gtaggttttg ccggtcttta
    16681 cggaatagag gccctttacc ttgactttgc cggttttgag cagcgcagcg gcgatcttcg
    16741 gggtgaacgc tttcttgcgt tcctcaaaaa atcggtcatt cttccacatg acgaactggc
    16801 aggcccggtt tccgcagtag tagtttttct tgccctcata gacagcctca ccacagcggg
    16861 gacacgtccc gataggaaca cgctcggcct gaaacaattt ctgcttttcc tcactgatac
    16921 aggaataggt ctgcaccagc tcccgcgcct gcgtctcgat gccgtccata aattcgtcag
    16981 gatcggcctg ccccttggcg atctcggtca gccggttttc ccattcggca gtaagctggg
    17041 gagaagtaag cgcctccggc agcaccacag ccagattgat gccgtccttg gtgggaatga
    17101 gctgcttgcc ttttctctgg acaaagccca tctgcaccag cttttccaga atggcggcgc
    17161 gggtggccgg tgtgcctaat cccttgcgct cggcatcctc cggcatatcc tcggccccgg
    17221 cccgttccat agcggacaaa agcgtatctt cggtgtaggg cttgggcggc gtggtgaaat
    17281 gctcggtgac agaggcggcc acgtctgcaa aggtctggcc ctcgcaaagc tccggcagct
    17341 cccgcaccgc ctcggcgtcc tcgtcggcgt cggtctttaa ggaagaacgg aaccggcgtt
    17401 caatctcttt ccagccgggg gaaaggacgg tcttgccctt ggcggtaaac tcctgcccgg
    17461 cacaggtaaa tgtggcggtg acggcctcat agatatgcgg cccggccacg gcacataaga
    17521 gcttgcagca gatcagggac agcagctttt tttcactctc ggccagagtg ccaaagccct
    17581 gcttcacaaa ttcagcggtg gggataatgg cgtggtggtc ggacaccttt ttgctgttca
    17641 ggatgcggtc aaactgcggg gaaaggttca ggcccttggc aaacggcacc agctcccaca
    17701 ggccggacac aaggctttcc acggcgggca gcatatcgtc ggtaagatat tgactatccg
    17761 ttctcggata ggtcagcagc ttctttagat acagtgcctg ggcatagtca agggtctgct
    17821 tggcggtata gccgaaaagc cggttagcct cccgctgcaa ggtggtaagg tcatagagct
    17881 tgggcggctg ttccgtcttt ttctcccgcg tcacggaaac gcaaacggcc tgcgcttttg
    17941 cacaagccgc cttgacggtg tttgcctcgg cagcgtcgga aaagcggctg cttaccgcgt
    18001 ccgcgccgcc cgccgtgata tggacgatgt gatatttctc tttcttgaaa tcggtaatct
    18061 tggcgtccct gtccaccagc atttttaagg tcggcgtctg gacgcggccc acagtcaggt
    18121 tcttgtggta caggacggaa aaaagccggg tggcgttaat gccgatcagc cagtcggcct
    18181 tggcccggca cagggccgat tgatacagcg gatcatagtc gccgcccggt ttcaggtggg
    18241 caaagccgtc cttaatagca ctgtcctcca tagaggaaat ccaaaggcgc tcaaagggct
    18301 tttggcatcc cgccatctgg tagacgaaac ggaaaatcaa ttccccctcg cgcccggcgt
    18361 cggtggcgca cacaaggccg gtcacgtcgg ggcgctgcat cagcgaacag agaacgtcaa
    18421 actgctgttt cttgtcgtcg gggacgatgt actgccatgt ttccgggaga atgggcaaat
    18481 cctcatagcg ccattttttg aaacgctcgt catagcgtcc ggcgtcggca aaggacacca
    18541 gatgtccgat gcaccagctc accagatagc cgttcccctc caaatagccg tcgtgtttct
    18601 ccgtggcccc gatcacgccc gcaatgctgc gggccacgct gggtttttcc gcgattacaa
    18661 gtttatatgt cattcctcgt cctcctgttc actcacaaaa tcagcttccg ggtcaaactc
    18721 ggtttcgggt tcctcgtcct ccaaatcctc gtcctcccca aaatcatact cgcccaaatc
    18781 gtcgctgccg gtggtttcag ccttgggctt tttgaacttg aaatagtaaa gggccgcacc
    18841 gccgcccgcc agcgccacca gcaggaacac gatcaggccg cccgcgccgc ccttgggttc
    18901 ttcctccggg gcttcgggtt cctccggctg gggttcctgc ggctccgggc cgctgcactc
    18961 ggtcaggttg gttttacata ccgggcaatt cgtgttcaca gccccggcct tgcatttcgt
    19021 ggtacaggag cagacagcag cgggggtgac ggtttccccg tcctccatca gcgtcagcag
    19081 atcggcttca tccacctgat ttaagaaatg gacagtttct tttccctcgt cgtcccggtc
    19141 aataatcaga taaaacacat tgccgttttt ggtttccacg gtgataaact gcttgccatt
    19201 tttcgcggcg ctgccgatgt cgtcaatcag tgacaggttc ccgtccgggg acagggcggc
    19261 gctggccccg ccgccattga acagttccag cagagaagca atttcctccg ggtcaatgga
    19321 gagggaaaaa ccgccctcgc cggtagccgc tacttccttt tcgctgacgg tcatatagtc
    19381 ggaatggaca tagccgatgc gctccggcaa gagaatttta atccagtcgc cgtctgtgcc
    19441 gactacctca accgtcgtcc cattgggaag ctgggtgaac gcggtcttgt caagccccgc
    19501 gccggtgcgg acattcagcc tgccgccgtt ggtggtgacg gtgccggtct gttcaggttc
    19561 ttttttgtcg gtgccggtgg tgatctggat gccgtcgtcc gtggcggcaa tgctcacctt
    19621 ggaaccgaac agcgcggaaa acaggtcggt gatgtccttg ccggtcagag ggccggtatc
    19681 tttttcggcc tcggtttctg tcccgtccgt ggcggtgccg gtttcgtctg tttcaggctc
    19741 caaaccgccc gtagcgggtt cggttgtagc ctccggcagc ggggccggtg ccgggtcatt
    19801 gccgcctgca agggcggtaa cgggaaaggc cgtgacgcag agcatcacgg ccagaagtgc
    19861 ggggagaata cgggaaatcc gtctggtctt attcttcatg ggcattgtcc tcctgttcat
    19921 ggtaaggggt tgcggcaggg gcggcctgca ttgcgccgct gcgtaagaac gcggcaaatt
    19981 cctccggctt catgttcatg ccgcgcacaa gctggatgat ctgcaaattc tcctgttcgg
    20041 ttttctgcgt ttccagctcc cgaagctgtt tctgaaactc cgcgatctta gacttggttt
    20101 tctggatgtc cttttcgatg cggtcaagtt tggttgccat aattgaaagc tcctttctgg
    20161 cgttaaggta aacgcccgta actgtaaaaa tgggattgcc agtagtttga agtaaggttc
    20221 gtgtaggaaa tcgggttccc gcaatgcagc atcacattgt tgcccacata caggccgacg
    20281 tgggacacgc cgggggtgtc gtaggttccc ttgaaaaata caaggtcgcc gggcttggcc
    20341 tgcgaactgg aaacaggggt gcagatgtta tataggccct gcgcccccag ccgtcccacg
    20401 ttccatccac tatgattgat tacccacgaa atgaagccgc tgcaatcaaa gctggtgctg
    20461 ggagagctgc cgccccacac atagggataa ccgatatatt tttccgcctc tttgagcatg
    20521 gcggcaaagg tggcatcctc caaagcctcc gggggtacgt cgtagtaggt cggcggcgtc
    20581 agggtggagg cgttggggta ctggctgccg gaaaacagat caggccggtt tcctagcgtg
    20641 gacatataca gggaatagaa acttagcgtt tcctcgtcca tgatatagac tggcacatgg
    20701 gacaggtcga agttttccag cgtgacgtag cagatgtaat agtcatacgg cacttggtag
    20761 gtgtctgtat gggtgttgcc ctccgcgtcc gtccatgtat cggtttcggt gcggtaacgg
    20821 gtttctacca cgacgttttc tgtcaggata tattgcttgt caaagagcat ggacaaatca
    20881 ccctgtacct ccccgatggt aaatatcccg tcatggagcg ccgacagaat ggaaatcagc
    20941 acataggggt catggctgat ctcgtcaagg tcaaaatggt attcgtcata gtcatgggtg
    21001 gcctcgtagt tgtccagata gtcttgcagc tcggcttcca gctccgcgta ggcgtcctcg
    21061 gcggccaaca tatcgctatc ctcggaaaag taagaggacg ccgtgatgcc gcttccggcg
    21121 ctgccgaaca tggcggtgca ggactgtaag ccgccgatga taagcagcat caaaagcccc
    21181 aacacaagga cggccagcgc ccctttccag tggcgggcca caaaggaggc cgcccgttca
    21241 ctttctttgg ctgcggcctt ggccgctttc gtggaggccc cggctgtctt ggccgcgccc
    21301 tgtgccgccg cgcctgcggt ctggcccgct gtccgggcgg ccttggcgta ctggcgcttg
    21361 atctgctgtt tctgccagaa ccgggaaatg gggttgctgg ccgcctgtgc gatctccggg
    21421 ttatcccgca aagatttctg gtagaaatac tccgcgttgg cagacaatag cctgcgctcg
    21481 gccttttccg cgtcccagta gggtttcagc ctgtggtgcc ggatggtgct gcggatggct
    21541 tttccggcct gccgctcggc aagttcctcc cccttatggc cgccctccac acccacgttt
    21601 tcatgctcga cttcatggat tttcccgtgg ccgaatagaa aaacctcgga aacaggacgg
    21661 cctgccggat tgggtttcag ggagggaggc cccttgtcct gtttgtcaaa gtgcaggacg
    21721 gtctttgctt tgccgcttgc ctcgtcaaag acggtttcct tgaccgggac gcgcttcttg
    21781 gggatggcgg cgcgggcctt atccagctta tcggcccgtt tgtccgattt tcggatatag
    21841 ggctgtaaat ccgggtcggc ccgttcttcc tccgacagtt ggagccgggc ggatggccgg
    21901 tgcagggcag cgtccccctc ggcggccaca gccccggcct gcttcgcggc tttcttggcc
    21961 ttatggccgt catggatggc gtcggcccgt tccagcagct tatcggcggt gccgcccggc
    22021 cccggcgcgg acaaatcctg ttcggcctcc cggctggaaa tgttctccac ctcgccggtg
    22081 gcctgatttt ccagcgtcag cccgtcgcgg gtcatggcct gcgtcacttt cgccttggct
    22141 ttcagctctt tcatggtggt tcacctcctt tgtggcgtcg ccgggtcata ctcccgccac
    22201 ctcgtttaag cgggtcgtca taatcttgta aagctgggtg ttgtgcggga tggggttggt
    22261 aaagggcagc accacgtttt caaagatcaa aaggccctcg ccgggttcgg aattgtcgat
    22321 gtacttctgc tgttcggtgg acaaattcaa gcgttccgca aggatttttc tatccccgga
    22381 cgcctgattg agcagcgtaa tgaagtcgct gttttcaagg atattttcaa tttccgggga
    22441 ggaaagcagg tctttgacgt tctgggtggc acctgttgga atgccgcccc atttcctaaa
    22501 ccgcttccaa atctccgcag aataggccgc cgtctgttcc tctttcaaaa gcaaatggaa
    22561 ctcgtcggca aaataccatg tggcctttcc ttggcttcgg ttggccgtga cgcgccccca
    22621 tatctggtct tggacaatga gcatccccag ctttttgagc tgcttgccca gctccttaat
    22681 gtcaaaggac acaagccggt tgccgatgtc cacgttggta cggtggttaa atacgttcag
    22741 ggagccggac acatacaggt caagggcctg cgccacccgg tcagcctccg ggacatgctg
    22801 atctaacaac gctttgtgga ggtcggacag gatcggcata ttctccgggc gggggtctgc
    22861 cagatagggc cggtagatca cgcgcacagc ccggtctatc acggtctttt caatggcttc
    22921 cagcccgttc ttgccgccca tgacaagctc acagaacgac agcacaaagt cggatttcag
    22981 ggccagcggg ttatcgtcct ccgaataatt caggttgatg tccagcgggt tcacaaagtc
    23041 cttgctggta ggcgacagcc ggattacctg tccgtggagc cgtttgacaa gggggtaata
    23101 ttcggcctcc gggtctgaaa taaaaatatc gtcaggcgtc gttaagaaca cactgacgat
    23161 ctcggattta cagctcatgg atttgccgct gccgggggtg ccgagcttca aggcattggg
    23221 gcaacgggcc tgcttcctgt ccagcataat catgttgcgg gacttggcgt tgatgccgta
    23281 atacatggcc gcgccgccct gaaaaagctc ctgcgtcaca aaggggacga acacggccac
    23341 gctggaagtc gtaaggctgc gctgtatctt gatctgattg acgcccagcg gcaggctgct
    23401 tgcaaggccc tgttcctgct ggtagtccag ccggacaagg ggacagttat gcttttgggc
    23461 cacgcctgcg gcttggaaca cgtcgttatt cagcttttgc ttggtgtcgg ccacgttgag
    23521 gacaaggaac gtcaactgga acagccgttc attgcgggtc tgcaactttg tcagcagctt
    23581 cttggcgtcc tcgccgtagg tggcaaggtc gctgggaaga atgtccatat cgtaaccgct
    23641 gcggacggcc tttttctgct ccgcaatctt catagcgtca aggtcggtaa ttttgcgctt
    23701 gatggtcttg atcgccttgg tctgttcaat ggcctgcaca tggagattga ctaaaacacc
    23761 gctttccgta ttgaggaagt cggccagcat atcgtccgac agctccgggg acaggatttg
    23821 caggaaactt accgcgccgt atttgccgcc catgccgaat gtctttgcat tgccgaagca
    23881 cagggaggac ggggcaataa aatccttggt ggagaggccg gacggggcca gccacttcca
    23941 gtcaaaattg aatatctcgc cgtccgggtg gtacacgcca tgcagcaggt tcagccgttc
    24001 ccagccgtcc atcacatggg ctacggcccc catgctcttg aaatagccta acaggtcaaa
    24061 gccgatgcgc ttcaatctgg ctcgcgccgc tttcacgctt tcggcctcga tggtgaatgt
    24121 caggtatttg gtctttacca tgccgttgtt gccgtttgca agctgctttt gcagaatgcc
    24181 ggtgtactcg gcccggatgt cgtcgaagtc gtccccctgc ggcgcgatct caaagctctt
    24241 ggcgtactgc accgggtcaa ctttccgatt gaggaaagaa aactggatat gaatggaggc
    24301 gtccacatag ttatacaggt cgcacagttt ttcaaagata gccgtctgcg tgtccggctg
    24361 ggcaagctga tagctgatgt ccgcaaactc aatgcacttg gaaaagacgc cgggcgatac
    24421 ccggcacacg ccgtccgggt acatgacctc gtaggggatg ctctgctgcg ccgtgtgggg
    24481 cctgccgtcg cccttatatt tacggattac cgcgtcaatt tcttttcgct gggcgcgggt
    24541 cagctttgtt ttttcggttt ctcttgggat ttcctttttg gacaatggcc tttacctcct
    24601 gttccatctg gtattgccgc acaagggcgg tgtaggtgtt gtcggtctga tagggacgct
    24661 cctttggctg gatgaagcag cactggatga tctgcataat caccacttca aggggctggc
    24721 cgtgacgctc gtacatggca agcaaaaagc ccggcagcat ggcaaatacc atgagcaggg
    24781 ccgccgcgct gttggtaaga tagtcacggg tgagaaaata taaggggacg ccgatcacca
    24841 gcgccccgga aaaacagatc agttgccgtt tggtaagccc aaacacaacc ttgcttttca
    24901 catgggttaa gtccttgggg acggttacat aagccatttg gcacctcctt tcacgtcagg
    24961 ataaggtcac attccacctc gttcccccac acgtcccagc cgggggtttt ctgtctggca
    25021 aacagctcca cgcggggcaa atcgcccgcc agctctacga ttttttcacg ggtcacatcc
    25081 ggctttttgc tgtgttgttc aatcgggctg ataataaact gatggacaga ggcagaacga
    25141 cgcttcggct tgccgtgtgt tgccagcagg cagatttccg cgtttcccct tgtccagcgg
    25201 cccagcccgt aaaaccatgt aaggctttta cggttctgtt tcagccatac aaaagccacg
    25261 gtcttgaatg aaaagcccca cgctttgatt aaccgcagag cttccggcag catggggaat
    25321 gtggcccaca aaaacagcag gcagtctttg gacgccagcc gttccaccgg cagggcgcac
    25381 agctcgtcaa tgctcatggt ggaatagtga ttttccgctg cgccctgtac gtttttgcag
    25441 tcataccgcc acggcgggtc agcgtaaatg atgttgtacg ttgaaataag caagttcctc
    25501 ctgtgtatgc ttggattttg ggatcaatgc gccccgaata cggcctttga tatactgccg
    25561 gtcttgaaca ggcagaagca cagcagcacc gtatagccca tacagcccca gattgcgccg
    25621 gatatgtcgg aagcggaacc gatattctgt atcagcaccg cgtagatacc caccacaacc
    25681 atgattaaaa aggcttggaa gcccagcgcc agcagggatt tcagataccc ttggcccatg
    25741 ccgcgccatt ccgcgttgcc cattgtggca agggggatag gccccagcga cgtgacaaca
    25801 tagatttcca ccatgcggcc ataggtcaca aggaaaatgc agatggaaag gatattcatg
    25861 gtgaggccca caacaagggt ttggaaccac agccccaaaa gagcgcccac gctcatagcc
    25921 tccaattggc tttcaaggtc tggtatcagg gtgttaaaat caatggatgt ctgcccgata
    25981 atcacaccgg cgctttgatt gacgacgctt tgggtggcgt cgaatacgcc catgacgatg
    26041 ttccatgtgt tcgttacgat caggattgca aacgcggatt tgaatatcca gcggaagaac
    26101 atggagctgt caaagtcgtg catattgttc ttttccgtta tcatctggat aagctcatag
    26161 cacatgacaa gggcgagtat ggccccggct atggggacga tcacggtttc cgacaggctg
    26221 tgcagcatat tgaaaatacc gctgttccat gcctgcgggg ttcgtcccac gtcggcggcg
    26281 atttctccga ctttggcatt taccgtatcg aacagcccgg acaggttgcc cgtgataccg
    26341 tccatgagga tgccttttat ccattcttca atgatttcac taatcacggg tagcacctcc
    26401 tttcccgcgc cctcccggtc tggccgggag gacgcggagg cgattagtta agggtttgga
    26461 cagggacaaa ggccaggatc agcccagcag cccggacagc aggggaacca gagtgacacc
    26521 gatcagggcc acgccgccgc cagcgacaag ctgcttgaca ccctgcgatt ttgcccccgg
    26581 attgtcctgc ccgtatccct ccaacagatt gatgccgccc catacgcaca gagcgccgcc
    26641 cagtccgatc acgatagtct gcaaagtagt aactgcggaa ttaaaaaatg ccataaattt
    26701 acctctttct gccggtttcc ggctcataat gattttttga ttttgttgtt ggaatgaaaa
    26761 aagccgctgt ttattcagcg gcggcgtcct cgtccgacag gtcgatttca tacaggtcga
    26821 atacttcatc ctgcgttacc acttgctgcc gtttccggcg cgtgatagtc aaaaatctgt
    26881 ccacgtcaaa ggtattcttc ttgtcggcgt cggcaaggta tttgtaccgg ggatgcttgg
    26941 taatatcgaa tttctccgat aaaaacgggc gcacaccccg tagctgcaag atacacttgc
    27001 cgccgtccat cacggcaatt tcgtcttggg acatcaactc ctttcctaat ttttgatagt
    27061 tcaatccgtg ggacgtttgg cttccccggt tctcgctggt gttgaaactg tcaatcgttt
    27121 ctttccccag cgcatcggaa atctccttgg catttttgcc gcgcccacta aggaaaagca
    27181 cactatcgca gttatccgaa atgatctcgg cggcgtcctt gtaaatcgct tttagctgcg
    27241 actggctctg caaaataata gaggccgata tttcacggct tcgaatggtg gcaatgagtt
    27301 tatcgaaatt gggtatttgg ccaatgttgg caaattcatc caaaatcagg cgtacatgga
    27361 caggcagccg cccgccgtac acatcatcag cccggtcaca aagcaggttg attagctggc
    27421 tttggagcat agccagaatg aaattaaagg tcgtgtccgt atcgctcata atgagataca
    27481 gcaccgtctt ttcgtccccg atggtgtcca gctccatttc gtcgtcctcc atcagctcgc
    27541 gtacctcccg gatgtcaaag ggcgcaagtc tggcaccgca tgaaatcagg atggaagaac
    27601 gggtcttgcc tgcggacagc aggaatttct tgtactggcg caccgcaaaa tggtcggggt
    27661 ctttctgctc caaacgctca aacatgagat cgacggggct ttgaaactcc gggtcgtcct
    27721 cgcgggcttc actggcgttt atcatttcca gcagcgttga gaagttcatt tcctccgctg
    27781 gggcctcgta ccagatatag ccgatcaggg ccgaataaaa caaccgttcc gacttcaccc
    27841 aaaaatcctc cgctgacttt tccccctcgc ccttggtgtt ggaaatcagg gtatttacca
    27901 gctttagcac gtccttttcc gaatggatgt agacaaaggg gttgtacttc atggacttgg
    27961 aaaagttaat ggtattcagg attttaatgc ggtagcccgc ctgctggaac atcctgccgg
    28021 tttccagaac caaagtgcct ttcggatctg tcaccacaaa cgaagtcgga tagtccgtgg
    28081 tcggaaagca ttgcatcagg ttgggtttca cgaaaaatct cgttttccct gaaccgctgc
    28141 caccgatcac cagcacgttt ttattccgcg ctgtcttggg gtctttgggc ctgccggtca
    28201 tggtaagccg ttccgtttgg gtgagcagga tattattttc aaacttgggg tcgatgtaag
    28261 gcgctatgtc tttggccgtc ccccagcggg cgctgccata ctccatgcct ttgcggtatt
    28321 tcttcacgtt cttgctcttg cagtacacca ccagccgcac gatcacggca ccggcgatac
    28381 cgatacacag gtcaaacagg tgcaggcttg gcagcgggtt ggcaaaagcc gccgcaaaac
    28441 catccgcaaa atgcaggagc ttccccgaca ggtcagcacc agcggccagc cggtacgcct
    28501 ccccgcattt cgcaaacagg tacacaaaaa tcagatacgg gaggttcagg atcagcaatt
    28561 tctttacgtc aggcttcaca gttccagccc cctttccttg attttctcct tggcccgctg
    28621 cttggtctgg gccgccgcct tttgcttcac agcggacaaa accttgcgga tagagggccg
    28681 ctgctccttg gaaagcgtct tggcggtaaa ctcccgaaac gccagatcaa ggttatctgc
    28741 atccttggcc ttgaaaatca caacgtagtg gggcggctgg gtggtcttat cttttcgtaa
    28801 ggtaaaatcc acgccgtatt tcttggcgca tggcttgaac gctccaatgt tggcctcggt
    28861 gatctcaatg ttggagagat cagccccatg ctttttaagc tgctttaggc tctgctggcc
    28921 gtggtgcagc gtgtttttgc ccttggcccg gtgttgcagg tactttttca gcgccgcttg
    28981 cagcacttga gccgtgagct tgcccgtttt catggacagg gcaatggtct tttgagtaac
    29041 ttcttcctgc aatcgcatcc ctccttgtca gaaagtcaaa agcggcgtct aaggcggaac
    29101 caccagacgc cgcaggtaga tataggtcaa atccgcaccc gcaggccgga aaggtcgtcg
    29161 gcccggatgc cgacaagata ccagttttcg gccatttcaa tgcgggtggg cctccatttg
    29221 ccgccgatca tcacgtcgaa acactccccg caatgcaggc cgccgtaata gtcggccaga
    29281 tcaaagcgga tgtcgtaacg gtctgttcgc tcgtcaaaaa tcaatgcgcc ctgcttcata
    29341 gggtcgtcct ccttttctcg ttacaggctt tcgccctcgc cgcaggaata gtccggcagg
    29401 ccggatttag gctttcccca atcgggctgt gccatgtcgt gcgcgacaag ggccgtgtag
    29461 tagttggaaa tggtgctggg ggcgttgaac agcatagccc gcaggtattg tttgatattg
    29521 cggactttcg ttgtgttctc ccgcagacag tcaaggacaa agcggatatg ttcgccggtc
    29581 agcttcatgt acttggattt caccagctcg gcagggtagt cgtccccggc gacacggatt
    29641 gtctttcggg cggtgcagac ggtttccagc atcaggtcta cgatttcgtc aagctgttca
    29701 cggtcaatgc tcctgtcctg aatgagatag gcatactcga tgttctccaa aatgatttcc
    29761 cgataaatcc gtacagcctc atcctttcct gtccttttcc gttccggcgg cgtagccgct
    29821 gcctcgccct ccataggggc aggggtcggg gatgggatag gaatggaatg ggtactttgt
    29881 ccatctgtaa ttattttttc tttttttgat aagtcagttc tttgtacttc tttatttaat
    29941 tgcgttggat tttccgacgc cggattatcc gatgtcggat tttccaacac cgggttatcc
    30001 gatgttggat tttccaatac cgggttttcc aataccggct gttcgtagat ggtatagacc
    30061 atatcggcca ttttgccttt tgcgtcacgg ccctgctcac gccggatata tccagctttt
    30121 tcaagctccc ataccgctgt cctgatcgcg tcgatgcttt cccggttgat ataggacagt
    30181 ccttttaggg tatagtccca atcctccggc aaggacaaca tctgcgacag caggcccttg
    30241 gcctttaagg tcaggttctt attccgcagg tggtgatttg acatcaccgt atagcctttg
    30301 ttcttttcca cacggaaaac tgccatagcg ttgttcgctc ctttcactgt gaatttgacc
    30361 gctgcatagt cggtcagctt gcccggctga tagggacact ctgcaaactg gcagaattga
    30421 tatttccaat ccgggcggta aaatcggcag gtgccgcaat cctccggcgc gtccgccccg
    30481 ccgccgtcgt agtgatcgaa accgggcttt tcctgcatca gccgttcaaa cgcccggtca
    30541 gggccggaag taaatagcat ggcgtcgcct cctttctcta tttgggcata aaaaagcggc
    30601 gtcctgttct cccatgacgg gaaagaggaa cgccgcgttt acggtgtcgt attcaattta
    30661 tgatgtgccg tatttggggt ggcgggtatt agcagtatgg ttcagcatat atgcggctca
    30721 atccgaaagt agtaaattgg tagtaaattg agttctcata tcctgcgaaa tgcccgtatt
    30781 tccggggctt tcagcgataa cctaaatttt tggaataaaa ctttcattat aggctactgc
    30841 aacatccatc caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac
    30901 gattttattt tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc
    30961 ggattgattt aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata
    31021 ttgcgtagct aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt
    31081 tgctggtgag aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac
    31141 ctctttcatg atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg
    31201 ataaggcaat tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac
    31261 ttctcgcagt gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt
    31321 ttctccaata ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt
    31381 ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa
    31441 tgaattgagc tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt
    31501 cattaccgca ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac
    31561 ccctggaatt gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc
    31621 tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac
    31681 cgatccataa aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg
    31741 tgcccagatc gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga
    31801 acgatataaa acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat
    31861 atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc
    31921 gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt
    31981 atatttgacg agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag
    32041 ttgttccagc attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa
    32101 tgtcgatccc ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg
    32161 aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta
    32221 aagacttccc atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac
    32281 cttctccggc tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct
    32341 gtccaacttc tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat
    32401 tctcagattt caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga
    32461 tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc
    32521 cttcatttac tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc
    32581 aatccgtatc gccttctagc gctttgttga ataataccgt gaataattta tctctgctta
    32641 tctccatacc caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg
    32701 aagagcagtt attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt
    32761 cgatttccgg atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag
    32821 taccagccga aatgttccct gtccgtttgg ctacactgtt cgtagcgacc ataccggttc
    32881 ctgcatcccc ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga
    32941 tcagataagc ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag
    33001 gcaaaatgtg atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat
    33061 ttttcatttc ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat
    33121 ctcctacgcc tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg
    33181 tcaaaaaatt aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag
    33241 cgatactcca tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt
    33301 cttcagtgat tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa
    33361 atgctagata accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct
    33421 cgccatagat ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc
    33481 aaatatcttc taatgaatag gtccagatac cattttctag tttgttttcc cactcataac
    33541 taccagcggc aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc
    33601 caaactcaat ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta
    33661 cttccttcat aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta
    33721 gtagcgctta caatgattat tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa
    33781 gaatacaata aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca
    33841 aaatctgatc aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa
    33901 aaaaagtttc ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat
    33961 atgatattgg tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag
    34021 gtaaagcttc tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg
    34081 gctctttatc tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg
    34141 cacttatttt tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt
    34201 ttctgattgc agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc
    34261 ctgcttatat gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc
    34321 aagtgatgat tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagtgt
    34381 tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt
    34441 tatttttcgg tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct
    34501 tggaagaggc aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat
    34561 tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt
    34621 tgtttttaga aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc
    34681 aatttcaagg agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg
    34741 ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg
    34801 gttctttgct ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag
    34861 gcggtagcgt gatgggactt tcattcatcc taccagctgt tttaggaact gtactggatg
    34921 ctcatacaaa ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt
    34981 gcacatgggc tccactgaca tgggtaatca taggcgaaat attcccacta gctgtccgcg
    35041 ggcgagcatc tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc
    35101 tttttcctgt gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca
    35161 tctgcttttt aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt
    35221 tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc
    35281 attcttaaag aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag
    35341 tttccagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac
    35401 ttgtgaccag ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc
    35461 cttgagcgat gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga
    35521 ggcggtaaaa acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta
    35581 caggttcata gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc
    35641 gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc
    35701 ctaaatggct taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat
    35761 agccattttc tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga
    35821 tgccatctaa agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc
    35881 gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac
    35941 acgcgctgat aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga
    36001 acaacagacg ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca
    36061 catcatctaa acaaatataa gaggcattta actcttcata agcagcattg atcatcacca
    36121 tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac
    36181 ttttcgtagg ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact
    36241 ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc
    36301 gtaattcatt ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg
    36361 tgatgacacc gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat
    36421 ctacatatgt gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga
    36481 ttgcttgccg aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg
    36541 gaatggttgt atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa
    36601 tatcttgata ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac
    36661 gagtctgcaa ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga
    36721 acaggttcac caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg
    36781 tgccgattag gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag
    36841 atcaaaatat caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat
    36901 tgcttatttt tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt
    36961 aataaaagat tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt
    37021 gcactaccag aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca
    37081 tttactgaaa aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc
    37141 ccctttaacg tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt
    37201 tcaggatctt gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg
    37261 tctaaactga aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc
    37321 attggatcta tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt
    37381 atagttttat gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact
    37441 tcaggtgccc agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg
    37501 cttgcagcat gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag
    37561 cctggaaccg atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct
    37621 gcccgttgcc gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca
    37681 ttttctttat tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat
    37741 gtttttctat tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat
    37801 ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga
    37861 aatcatgttc aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca
    37921 gctgattgcc gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac
    37981 aatcataaac agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata
    38041 agtgttgcat cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta
    38101 ttaaaggtat tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc
    38161 gtaaaatagc cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc
    38221 aatcgatggg actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat
    38281 tccattcatc aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat
    38341 cacaagtagc aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg
    38401 tcgctgtatc atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat
    38461 caatatagtt atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac
    38521 gatcccattc agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa
    38581 ttgaatcatc cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat
    38641 aagcactctt agcccccact tgccatggac cgtctagctc attccctaaa caccataatt
    38701 taacgccaaa aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt
    38761 ttggaaaatt acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat
    38821 tcaccgtgaa tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc
    38881 caaaagaatt tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt
    38941 tcctacctac agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa
    39001 tgacacctaa ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat
    39061 cagctgtttc atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa
    39121 caaatgagcc aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta
    39181 ctttcacttc ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag
    39241 atacaagttt ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga
    39301 gcagtacaaa tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata
    39361 ctttccatgc actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca
    39421 catatatcca ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg
    39481 aaagtacgat tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta
    39541 acagactaag gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa
    39601 tgatatttcc tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag
    39661 caggcacgat agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt
    39721 aatcagaaac cacaatcaac tgtccataaa atttagtctc aactattttc atcttcctca
    39781 ctctttcatt ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac
    39841 cactagtaat ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat
    39901 gttctgccct gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt
    39961 caatatcaat ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga
    40021 taaaagagta ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa
    40081 tccatcaatg cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat
    40141 catgaagata ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag
    40201 catgccaagc aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat
    40261 cattgtcagc aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa
    40321 tgcatacccc gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat
    40381 cactgtattt ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc
    40441 tagggtatag ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt
    40501 aaaagaagaa ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat
    40561 actaatcgtt cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt
    40621 aatcgagctt tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg
    40681 acatgtacac attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca
    40741 gtgctgatgc atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa
    40801 cagctgtttc tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca
    40861 cttgcatcga accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag
    40921 gtagcgtaat gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat
    40981 acaaagaggg agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat
    41041 tcttccaaac agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt
    41101 gagggatcgg caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa
    41161 gcaacataga aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg
    41221 tcgaacgaca aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac
    41281 caacgataat ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat
    41341 taactgctcg ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg
    41401 atgtaaagaa aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga
    41461 cagtaaataa taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag
    41521 gacgattcca atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa
    41581 aatatacaat cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt
    41641 tgcaataaca cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac
    41701 agtctatcct gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg
    41761 catcgttcat gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga
    41821 tgatatcgtt attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt
    41881 ccatttgttt cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta
    41941 ctgaatccaa gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg
    42001 tattccaata tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg
    42061 gaatggcata tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg
    42121 gtaaggtagt cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc
    42181 cattgatttc aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa
    42241 tcgattctgg accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa
    42301 ggttctcagg agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat
    42361 ttgctaacat ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata
    42421 catttttact actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag
    42481 gtggttgttc agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca
    42541 tgccttgtac ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag
    42601 ccgtttcttt aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt
    42661 gtaccgctcc gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag
    42721 gtgctttttt agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga
    42781 tatccatttc aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt
    42841 gcaatatttc accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat
    42901 cagaatcaga ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta
    42961 ccaacaaaga gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat
    43021 aaacgacatc tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct
    43081 tcagctttta catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt
    43141 gcttttgtat caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca
    43201 gacaagtgaa cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt
    43261 tctttgtcat ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc
    43321 gcatagtttg ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct
    43381 tgcataaatg gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt
    43441 ggtgcaataa cgtttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc
    43501 aataaagtga ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct
    43561 agtaaaggag gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac
    43621 caaatattcc attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg
    43681 actgtatcgc acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca
    43741 agataatgag aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct
    43801 atttgatcgt acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc
    43861 cgattcgaac ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta
    43921 gccgtttcct ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga
    43981 ccgtccattt cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc
    44041 gaacgcctta aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta
    44101 cgagctgcat ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca
    44161 tgctgacacc agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa
    44221 gctaaatcca accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat
    44281 cctgacacaa aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag
    44341 tgaatgacgt cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc
    44401 ccttcatata ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact
    44461 ttgcctacac gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa
    44521 tttatacatg aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta
    44581 cttatttttt atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta
    44641 atataacttt tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata
    44701 tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac
    44761 aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa
    44821 agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa
    44881 catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat
    44941 cgatattagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc
    45001 tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga
    45061 ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg
    45121 cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga
    45181 ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa
    45241 tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac
    45301 gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa
    45361 tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa
    45421 tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca
    45481 tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa
    45541 aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc
    45601 ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt
    45661 attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct
    45721 tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat
    45781 aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc
    45841 catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt
    45901 gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac
    45961 catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata
    46021 cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga
    46081 tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg
    46141 catcaacagc tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag
    46201 cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt
    46261 gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc
    46321 ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat
    46381 attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa
    46441 ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc
    46501 ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt
    46561 atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt
    46621 atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc
    46681 aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt
    46741 aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc
    46801 ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa
    46861 atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct
    46921 tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa
    46981 tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac
    47041 atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga
    47101 agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga
    47161 tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa
    47221 ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac
    47281 taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc
    47341 ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc
    47401 agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac
    47461 tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat
    47521 gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa
    47581 cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc
    47641 atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt
    47701 ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc
    47761 ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac
    47821 cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc
    47881 agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag
    47941 cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt
    48001 catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg
    48061 aaaaagcaca tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat
    48121 cctttcttat tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg
    48181 agacacgaag aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct
    48241 actacaatcg tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca
    48301 acataatcaa tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat
    48361 ccccaaagaa acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga
    48421 caagatatac cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat
    48481 acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt
    48541 ctcgccttgt tttttattgt atacattaga tatggagcag gttaccataa agatgattat
    48601 tagtcgttat taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt
    48661 tggacgtttg aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt
    48721 gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt
    48781 gatttttttc ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag
    48841 cagttgtgtt cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa
    48901 gaattccact actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc
    48961 gattagcttc atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat
    49021 gttaccatct ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga
    49081 tttacctttt atcacttaat agtataacat aatttcatga gaaaaaaata caattttttc
    49141 ttgaccatct cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag
    49201 tacacaataa agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat
    49261 gatgagattg gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc
    49321 aaaaaagaag tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg
    49381 gatgatatcc aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag
    49441 catcagcaaa tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa
    49501 cagacacttg atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg
    49561 tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa
    49621 tacggaaaaa aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg
    49681 tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa
    49741 gaaccagaag atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttatct
    49801 tacagctggc ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc
    49861 gatgaacggt tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta
    49921 aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta
    49981 aaatgaccta ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg
    50041 tctgctccat tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa
    50101 acgtataagg tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt
    50161 ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca
    50221 aattatcgcg tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg
    50281 ctgcaaccat atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc
    50341 ctgcttgttt tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag
    50401 atggaaaaag ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac
    50461 ttcgccccaa cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt
    50521 ctgtaattga ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt
    50581 cacaaaaaac tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac
    50641 gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct
    50701 ctttttcttt ctgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg
    50761 cttcaatgat cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca
    50821 ctccaatgct tttcgttttt ccataactta actgtctggc atttgcatta gggatataat
    50881 cgacttcttt catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc
    50941 cattgattac acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa
    51001 tgtttgccac tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac
    51061 agctggaccg cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg
    51121 cccgccccaa tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac
    51181 tatattccta ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac
    51241 aaacgcataa aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc
    51301 aagccctcct tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt
    51361 gttttgccaa agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat
    51421 tgaatacatt ataatgatac tgtaaatata tctttattat tagtgaatgg atatttactt
    51481 ttaatagata aaatattata actattaaaa ataactattt tcattataat tacaca
//
LOCUS       DAZUMT010000005        51191 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000005 DAZUMT010000000
VERSION     DAZUMT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.005"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..561
                     /locus_tag="ACG8FM_000265"
     CDS             190..561
                     /locus_tag="ACG8FM_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS5092959.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(835..1527)
                     /locus_tag="ACG8FM_000266"
     CDS             complement(835..1527)
                     /locus_tag="ACG8FM_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5092960.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1530..2315)
                     /locus_tag="ACG8FM_000267"
     CDS             complement(1530..2315)
                     /locus_tag="ACG8FM_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5092961.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2552..3952
                     /gene="lacG"
                     /locus_tag="ACG8FM_000268"
     CDS             2552..3952
                     /gene="lacG"
                     /locus_tag="ACG8FM_000268"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS5092962.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3977..4294
                     /locus_tag="ACG8FM_000269"
     CDS             3977..4294
                     /locus_tag="ACG8FM_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5092963.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4307..5980
                     /locus_tag="ACG8FM_000270"
     CDS             4307..5980
                     /locus_tag="ACG8FM_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS5092964.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5994..7625
                     /locus_tag="ACG8FM_000271"
     CDS             5994..7625
                     /locus_tag="ACG8FM_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS5092965.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8116..9681)
                     /gene="guaA"
                     /locus_tag="ACG8FM_000272"
     CDS             complement(8116..9681)
                     /gene="guaA"
                     /locus_tag="ACG8FM_000272"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5092966.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9740..10663)
                     /gene="coaA"
                     /locus_tag="ACG8FM_000273"
     CDS             complement(9740..10663)
                     /gene="coaA"
                     /locus_tag="ACG8FM_000273"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS5092967.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10859..11464
                     /locus_tag="ACG8FM_000274"
     CDS             10859..11464
                     /locus_tag="ACG8FM_000274"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5092968.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11772..12788
                     /locus_tag="ACG8FM_000275"
     CDS             11772..12788
                     /locus_tag="ACG8FM_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5092969.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12929..14230
                     /locus_tag="ACG8FM_000276"
     CDS             12929..14230
                     /locus_tag="ACG8FM_000276"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS5092970.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14276..14938
                     /gene="deoC"
                     /locus_tag="ACG8FM_000277"
     CDS             14276..14938
                     /gene="deoC"
                     /locus_tag="ACG8FM_000277"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS5092971.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14959..15357
                     /locus_tag="ACG8FM_000278"
     CDS             14959..15357
                     /locus_tag="ACG8FM_000278"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS5092972.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15423..16505
                     /locus_tag="ACG8FM_000279"
     CDS             15423..16505
                     /locus_tag="ACG8FM_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5092973.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16717..18279
                     /locus_tag="ACG8FM_000280"
     CDS             16717..18279
                     /locus_tag="ACG8FM_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5092974.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18272..19405
                     /locus_tag="ACG8FM_000281"
     CDS             18272..19405
                     /locus_tag="ACG8FM_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5092975.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19405..20361
                     /locus_tag="ACG8FM_000282"
     CDS             19405..20361
                     /locus_tag="ACG8FM_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5092976.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20460..21626
                     /gene="deoB"
                     /locus_tag="ACG8FM_000283"
     CDS             20460..21626
                     /gene="deoB"
                     /locus_tag="ACG8FM_000283"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS5092977.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21650..22468
                     /locus_tag="ACG8FM_000284"
     CDS             21650..22468
                     /locus_tag="ACG8FM_000284"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5092978.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22485..23192
                     /gene="deoD"
                     /locus_tag="ACG8FM_000285"
     CDS             22485..23192
                     /gene="deoD"
                     /locus_tag="ACG8FM_000285"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5092979.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23490..24176)
                     /gene="gpmA"
                     /locus_tag="ACG8FM_000286"
     CDS             complement(23490..24176)
                     /gene="gpmA"
                     /locus_tag="ACG8FM_000286"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5092980.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24348..24929)
                     /locus_tag="ACG8FM_000287"
     CDS             complement(24348..24929)
                     /locus_tag="ACG8FM_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5092981.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25062..25745)
                     /gene="rpiA"
                     /locus_tag="ACG8FM_000288"
     CDS             complement(25062..25745)
                     /gene="rpiA"
                     /locus_tag="ACG8FM_000288"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS5092982.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26064..26477
                     /gene="rpsL"
                     /locus_tag="ACG8FM_000289"
     CDS             26064..26477
                     /gene="rpsL"
                     /locus_tag="ACG8FM_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS5092983.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26561..27031
                     /gene="rpsG"
                     /locus_tag="ACG8FM_000290"
     CDS             26561..27031
                     /gene="rpsG"
                     /locus_tag="ACG8FM_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS5092984.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27125..29212
                     /gene="fusA"
                     /locus_tag="ACG8FM_000291"
     CDS             27125..29212
                     /gene="fusA"
                     /locus_tag="ACG8FM_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5092985.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29384..30571
                     /gene="tuf"
                     /locus_tag="ACG8FM_000292"
     CDS             29384..30571
                     /gene="tuf"
                     /locus_tag="ACG8FM_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5092986.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30978..31286
                     /gene="rpsJ"
                     /locus_tag="ACG8FM_000293"
     CDS             30978..31286
                     /gene="rpsJ"
                     /locus_tag="ACG8FM_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS5092987.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31317..31946
                     /gene="rplC"
                     /locus_tag="ACG8FM_000294"
     CDS             31317..31946
                     /gene="rplC"
                     /locus_tag="ACG8FM_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS5092988.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31974..32597
                     /gene="rplD"
                     /locus_tag="ACG8FM_000295"
     CDS             31974..32597
                     /gene="rplD"
                     /locus_tag="ACG8FM_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS5092989.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32597..32887
                     /gene="rplW"
                     /locus_tag="ACG8FM_000296"
     CDS             32597..32887
                     /gene="rplW"
                     /locus_tag="ACG8FM_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS5092990.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32926..33759
                     /gene="rplB"
                     /locus_tag="ACG8FM_000297"
     CDS             32926..33759
                     /gene="rplB"
                     /locus_tag="ACG8FM_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS5092991.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33802..34080
                     /gene="rpsS"
                     /locus_tag="ACG8FM_000298"
     CDS             33802..34080
                     /gene="rpsS"
                     /locus_tag="ACG8FM_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS5092992.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34103..34450
                     /gene="rplV"
                     /locus_tag="ACG8FM_000299"
     CDS             34103..34450
                     /gene="rplV"
                     /locus_tag="ACG8FM_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS5092993.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34464..35120
                     /gene="rpsC"
                     /locus_tag="ACG8FM_000300"
     CDS             34464..35120
                     /gene="rpsC"
                     /locus_tag="ACG8FM_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS5092994.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35123..35557
                     /gene="rplP"
                     /locus_tag="ACG8FM_000301"
     CDS             35123..35557
                     /gene="rplP"
                     /locus_tag="ACG8FM_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS5092995.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35547..35735
                     /gene="rpmC"
                     /locus_tag="ACG8FM_000302"
     CDS             35547..35735
                     /gene="rpmC"
                     /locus_tag="ACG8FM_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS5092996.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35760..36026
                     /gene="rpsQ"
                     /locus_tag="ACG8FM_000303"
     CDS             35760..36026
                     /gene="rpsQ"
                     /locus_tag="ACG8FM_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS5092997.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36084..36452
                     /gene="rplN"
                     /locus_tag="ACG8FM_000304"
     CDS             36084..36452
                     /gene="rplN"
                     /locus_tag="ACG8FM_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS5092998.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36489..36797
                     /gene="rplX"
                     /locus_tag="ACG8FM_000305"
     CDS             36489..36797
                     /gene="rplX"
                     /locus_tag="ACG8FM_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS5092999.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36825..37364
                     /gene="rplE"
                     /locus_tag="ACG8FM_000306"
     CDS             36825..37364
                     /gene="rplE"
                     /locus_tag="ACG8FM_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS5093000.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37383..37568
                     /locus_tag="ACG8FM_000307"
     CDS             37383..37568
                     /locus_tag="ACG8FM_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS5093001.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37604..38002
                     /gene="rpsH"
                     /locus_tag="ACG8FM_000308"
     CDS             37604..38002
                     /gene="rpsH"
                     /locus_tag="ACG8FM_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS5093002.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38034..38570
                     /gene="rplF"
                     /locus_tag="ACG8FM_000309"
     CDS             38034..38570
                     /gene="rplF"
                     /locus_tag="ACG8FM_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS5093003.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38731..39087
                     /gene="rplR"
                     /locus_tag="ACG8FM_000310"
     CDS             38731..39087
                     /gene="rplR"
                     /locus_tag="ACG8FM_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS5093004.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39108..39608
                     /gene="rpsE"
                     /locus_tag="ACG8FM_000311"
     CDS             39108..39608
                     /gene="rpsE"
                     /locus_tag="ACG8FM_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS5093005.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39623..39802
                     /gene="rpmD"
                     /locus_tag="ACG8FM_000312"
     CDS             39623..39802
                     /gene="rpmD"
                     /locus_tag="ACG8FM_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS5093006.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39846..40286
                     /gene="rplO"
                     /locus_tag="ACG8FM_000313"
     CDS             39846..40286
                     /gene="rplO"
                     /locus_tag="ACG8FM_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS5093007.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40286..41581
                     /gene="secY"
                     /locus_tag="ACG8FM_000314"
     CDS             40286..41581
                     /gene="secY"
                     /locus_tag="ACG8FM_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS5093008.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41643..42290
                     /locus_tag="ACG8FM_000315"
     CDS             41643..42290
                     /locus_tag="ACG8FM_000315"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS5093009.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42481..42699
                     /gene="infA"
                     /locus_tag="ACG8FM_000316"
     CDS             42481..42699
                     /gene="infA"
                     /locus_tag="ACG8FM_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS5093010.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42732..42848
                     /gene="rpmJ"
                     /locus_tag="ACG8FM_000317"
     CDS             42732..42848
                     /gene="rpmJ"
                     /locus_tag="ACG8FM_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS5093011.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42866..43231
                     /gene="rpsM"
                     /locus_tag="ACG8FM_000318"
     CDS             42866..43231
                     /gene="rpsM"
                     /locus_tag="ACG8FM_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS5093012.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43259..43648
                     /gene="rpsK"
                     /locus_tag="ACG8FM_000319"
     CDS             43259..43648
                     /gene="rpsK"
                     /locus_tag="ACG8FM_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS5093013.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43728..44666
                     /locus_tag="ACG8FM_000320"
     CDS             43728..44666
                     /locus_tag="ACG8FM_000320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS5093014.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44694..45074
                     /gene="rplQ"
                     /locus_tag="ACG8FM_000321"
     CDS             44694..45074
                     /gene="rplQ"
                     /locus_tag="ACG8FM_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS5093015.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45346..46185
                     /locus_tag="ACG8FM_000322"
     CDS             45346..46185
                     /locus_tag="ACG8FM_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5093016.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46161..47030
                     /locus_tag="ACG8FM_000323"
     CDS             46161..47030
                     /locus_tag="ACG8FM_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5093017.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47020..47817
                     /locus_tag="ACG8FM_000324"
     CDS             47020..47817
                     /locus_tag="ACG8FM_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5093018.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48020..48766
                     /gene="truA"
                     /locus_tag="ACG8FM_000325"
     CDS             48020..48766
                     /gene="truA"
                     /locus_tag="ACG8FM_000325"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5093019.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49430..49624
                     /locus_tag="ACG8FM_000326"
                     /pseudo
     CDS             49430..49624
                     /locus_tag="ACG8FM_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49812..50588)
                     /locus_tag="ACG8FM_000327"
     CDS             complement(49812..50588)
                     /locus_tag="ACG8FM_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5093020.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50711..51118
                     /locus_tag="ACG8FM_000328"
     CDS             50711..51118
                     /locus_tag="ACG8FM_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFS5093021.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        16266 a         9299 c        11088 g        14538 t
ORIGIN      
        1 gattctataa tatttttgat ttatatagga aattaaaaat attatagaat caacactcag
       61 agaacgggtt ggtttttaat agatttaata agtactgata atgaaatttc tcctggttgg
      121 gaaaacgaag ttggttaccc tgtagaagaa ttttttcaag attactttgc ggagtatgcc
      181 gataggataa tgagagatta cgtaacagat aaaactcaag aagtcatcaa tcaatcccag
      241 tcattaaatg aattatctga taaggcgaaa gaagaagcag ttgccattat ttcttcaggg
      301 attagtgact atattgatat gagtggtgaa atcaattatg gcgataaaga caaaattgta
      361 cgcatgataa aagcttttat caaaactttt gatgtgaagg cagatcttaa gtttaatgat
      421 acttacctag taggaaaatt aattaatatc cttgggacag agaaagatac ggaaacgttt
      481 atttcctctt tgtctatgtt gtttacagat aagcattttt caactcagct tggtggagag
      541 gaactagtgg aaatctttta accattacgc ccagcattaa taaaagtata tcaaaaaagt
      601 actcctgatg atttttaaga ttggtttgag aaataatgtg aaaacactat ttttaatgtt
      661 aattatagat agagatgaac gcgtcatatg gttaaaagaa tgaaaaagtc aaagatacac
      721 tgctatattt cactaaggat atagcagtgt atctttcatg tcgtttcact ggcagttttt
      781 tataaaagga ggtgtgcatt tcagttgctt ttctttggta gttagttgaa tcgtttaaga
      841 attaacggat gtggcagaca ggtgctctgt ccaaaaacca gtaaaatctt tgatggttcc
      901 atctttattt agctgtgcct gttcaaatac tgttttgttt ttaccgaatg gatcaattgc
      961 aataattgtt ccgatccccg cacgtttggc agcaagtaat cctgagtaag catcttcgat
     1021 aacaagacaa tcagccggag ctaagctcag tttgtctgcc gctttaagaa aaatatccgg
     1081 ttgcggtttc ccagggaaag agccgtcgtc atagacaatc ttgtcccagt caaaccagcg
     1141 ggctaaatcg aaaatatcaa aataaaaagc aacattctct ttgacagtag ccgtggcaat
     1201 cgttaatgga atctgttggt cagtcagctg gtcaagagtc gcttcaagcc cttttgtcaa
     1261 ctgaagctcg ttttcatttt gcaagcaaag catacgataa tagctttctt tttcttctga
     1321 caatacggcg atctcagagg cggtcaaatg ctctgaaatg aaatgtttca aaatctccgt
     1381 attcgtccga ccatgaatat tttttaaaat atcctcgtct gataaaccat cttttgtatg
     1441 atcatgtatc atatgcagcc aagcagcttc atgcagatga gagtccaaaa acattgttcc
     1501 gttgaaatca aatataatcc ctttcatagt tacgctccta tagtggtatt ttcggttgaa
     1561 tcagcaattt gcagatcgac atattgtgca tagtgctctt gaacctcccg cgagatctgg
     1621 cgatccgtaa tgagcagatc gaaatcatgc aaattataaa acacataaaa atcatctcga
     1681 ttgaattttt tattatcaac gagtagatat ttcttacggg aattatttag agcgatctgc
     1741 tgtgcttctc cttcttcggt actgtaagta gagatcgctt cgttgtgaac gccatttgaa
     1801 ctgataaatg cttttgtaaa ctttagtctt cttaaattgt cgttggtgat cggtccgaca
     1861 aatgcgccgg tatttttccg gaaatcaccg ccaatcagta gaatttctgg tccatctgct
     1921 tgagcaagaa tatcaaaaac tggataatta tttgtaatga tccgaatttt cttgttcagt
     1981 aaatgcgtcg ccaatagttc gattgttgta cctggtccta agaaaatcgt ttctccctcg
     2041 tgaatcaagg aggctgctgt ggcagcaatt tctttttttt cttgaatctg cagagtggct
     2101 ttttcagtgt gagaaagttc ttgatctaaa gtgtaggaaa gactttgagc ccctccatgg
     2161 acacgcagca gcttgcccga tttttctaat tcatctaaat ctctgcgaac ggtcatatca
     2221 gaaacatcta attgattgat gatttcatta acggtaagga ttcctttttt attgaccatt
     2281 tctacgatag taagcaatct ttcttttttc aacattgtct tcacctctaa tttaaggata
     2341 atctatcata taaacggaca acagtcaatc taattcgaac aaaagtaaac atttagggtg
     2401 gtttttatgt ttgttacaat agattttatt gctgttttct ggtttttatg attatttgtg
     2461 tttgtttttg ttgacaaatg ttttggatgc gtttacaatg caatcagtta gaaagagcga
     2521 aaactgatta tagatttgag gagagatgta catgcttaaa ttaccagaag attttatttt
     2581 tggcggagcc acagcagcct atcaggttga aggagccact aaggaaggtg gcaaaggcgc
     2641 tgttgcttgg gatgactttt tagaggaaca agggcgattc agtcccgatc cggcaagtga
     2701 tttttatcat caatatgcaa aggacattga attgtgtgaa cgttttggtg taaatggtct
     2761 aagattatcc attgcttgga gccgcatttt tcctgatgga gctgggaaac caaatccaga
     2821 aggaatcgct ttttatcatc gtgtgttcga agaatgtaag aaaagaaacg taacgccgtt
     2881 tgtgacgttg catcattttg atacccctaa acgtttgttt gaccatggag attttttgaa
     2941 tcgagaaaca atagaagcat ttgtttccta tgccattttt tgttttcatg aattcaaaga
     3001 agtcaaggtg tggagtacct ttaatgaaat ctatccagtt gcaaccaatc aatatttgtt
     3061 aggtgttttc ccaccaggaa tcaaatatga ttttacaaag atcgtcgcct gtcttcataa
     3121 tatgatggtt gctcatgccc gtgtagtgaa ctatttcaaa gaaaacgaac ttccgggtga
     3181 aattggtgta gttcactcat tagagacgaa atatgcggca acagacgcgc cggaagataa
     3241 gcatgctgcc tttttagacg atgcattgtc gatccgtttc ttattagatg ctacctattt
     3301 aggctattat tcaactgaaa ccttgactgc tcttgatgag atatgtgaag caaatcaggc
     3361 cagctatcat tttcctgaag aagatttcgt tgaattgaaa aaagcatcca cgcgtaatga
     3421 ctacttaggg atcaatcact accaatgcca ttttgtcaaa gcttacgatg gagaaaatgc
     3481 aatccatcat aacggtacgg gtgaaaaagg gacctctgtt tataaagtga agggaatcgg
     3541 agaacgtatt tataaagaag gaattcctcg aactgattgg gactggctga tttatccaga
     3601 aggtctatat gacttgttgc tgcgaattaa aagcgattac ccacattaca acaaaatcta
     3661 tatcactgag aacgggatgg ggtataaaga tcagtttgaa gatggcatta ttatggatca
     3721 gccgagaatc gactatttga gagtctattt ggaatcctta agcaaggcga taacagccgg
     3781 ggtaaatgtg aaggggtatt ttttgtggtc gcttatggat cttttctcat ggacaaatgg
     3841 gtataacaaa aggtatggct tgttttatgt tgattttgaa acccagaagc gttacccaaa
     3901 agaatcagct tactggtaca agttggtcag tgaaaccaaa acgattattt aataagtgat
     3961 aatggaggaa gaaaagatga ataaagagga attacaaatg cttggttttg agatcgttgc
     4021 atattcaggc gacgctcgca gtacattatt gaaattattg aaagaagtaa gacaaggaaa
     4081 ctttgaacat gttgattcgg cgttgaaaga agccgacgaa aatttaaccc ttgcacataa
     4141 ttcgcaaaca aaaattttag cggaagaagc atctgggaaa gagatggaga tgggattcat
     4201 ttttatccat gggcaggatc atttaatgac cactttattg ctgagagatc tgattcaaga
     4261 ctttattgta ctgtacagac agaatcaagg ataggagaag aacgctatgg atggaatcat
     4321 taaacaaatc gaaaaaggga aaccattttt tgaaaaaatc tcacgtaata gttatttagg
     4381 agccgttcgt gacggatttt taacagcgat gccagctatt ttattttcaa gcattttcat
     4441 tttgattgct gcggttcctg agatttttgg ttaccaatgg cctgaatctg tctccacatg
     4501 gctatggaaa gtctataatt attcgatggg tgttgtaggg ttactcgttt cagcaaccac
     4561 tgctcgctgc ttggctgatt cgatgaatcg tcgcttaccg aaaaacaaag taatcaacac
     4621 cacctctgtg atgctggcct ctgtggtaag tttcctgttg ttaagcgtct ctccgattga
     4681 aggtggattt tcaacggaat atatggggac aaagggacta ctggcttcct ttgtcgctgc
     4741 atttttaaca gttaatttat ataagttttg tgtgctgaaa gatattacca ttaaaatgcc
     4801 taaagaagtt cctggaacga tttcgcaaac cttccgagat atttttcctt tctctttttc
     4861 agtatttgcg gccgttttaa ttgatttagg cattcgacaa atcttccaaa tctcatttgc
     4921 agaagcaatc attacggtgc tgcagccgct atttacagca gctgacgggt acttaggaat
     4981 cgctattatc tggggagcga tggcgctgtt ttggtttgtc ggtgttcatg gaccgtcgat
     5041 cgtggagccg gcaattgcgg caattatcta tgccaatgtg gaaaccaatc tgcaattgtt
     5101 caattccggt gaacaagctt ctaaagtact gacggtgggt ttagggaatt ttgtaggaac
     5161 tatgggagga actggggcaa cattggttgt tccttttatc ttcctactct ttgctaagtc
     5221 taagcagttg aaggcagttg ggaaagcttc ctttattcca gtaagtttcg ctgtcaacga
     5281 accattgcta tttgcggcac ccatgatttt gaacccttat ttttttattc catttttgtt
     5341 aacacctgtt gtaaacgttt gcctgttcaa gttttttgtc gatgtattgg gcatgaacag
     5401 ctttatgtac gttttgcctt gggcaactcc agcaccgatc ggtttggttc taggaacggg
     5461 catgggtatc ttagcttttg ttctagcagc ggttctaatc gtcgttgact tcctgatcta
     5521 tctaccattt tgcaaagcat acgatgctgt cttagtagag aaagaagcac aaaaagcaga
     5581 ggtgatcgca accgaggaag gtgcttctgc cgatgtacgt gaggaagtac ctgtggcagc
     5641 gagtcaagtt atcagcgatg caaataaaga aatcaatgtg ttggtacttt gtgctggcgc
     5701 agggacaagt gcgatgttag ctaatgcttt aaaaaaaggt gcatctgaat cgaaagcacc
     5761 gatcagtgca atggcgggtg cttatggttc tcattacgaa atgctgcagg attttgatat
     5821 ggtggtctta gcaccgcaag tcaattctta ctatgatgat atcaaagaag acaccgatcg
     5881 cttaggaatc aagctggttg cgacaaaagg agcagaatat atcggattaa cgagagatcc
     5941 tgaaaaagct gttcagttta ttctgaacca atttcaataa aggaggagcc ataatgaaaa
     6001 tgggaatttg gttgcgtaaa gctgttttat ttgcagctgt gttaggggct ttgggggctg
     6061 tttcaaaaac tgttgaagca tctgccagcg aatttattcg cggagcagat atttcgatcc
     6121 tgtcagatat ggaaaaatct ggtgcaaaat attatgaaga cgatatttca aaagatgcct
     6181 tagagatttt aaaaaataat ggtgtcaact atgttcgact gcgtttgtgg caggatcctt
     6241 ataatagtaa tggagaaagt tatggagcag ggacaaatga tctggaaacg acgattgacc
     6301 ttgcaaaacg agcaaaaaat ctgggactta aggtgctgct ggattttcac tacagtgatt
     6361 tttgggttga tcctgggaaa cagaatcttc ctaaagcatg gcaaggtctg acctttgaag
     6421 aaatgaatac ggcattgtac gactatacaa agaacgttct gtcggaaatg aagcagctgg
     6481 acgtttatcc tgatatggtt cagatcggca atgaactgaa tagcggaatg ctctggcctt
     6541 atggcaagtc ttggggagaa ggcgggggag aatttgatcg attggctgct tttttgaagt
     6601 ctggtatcca agctgttcga gacactcaac ctaaaacaac tccagtaatg ctccacttag
     6661 ctgatggtgg cgatacagga gcatttactt ggtggtttga tgaaatcacg agtcgaggtg
     6721 tgtcatttga tttaattgga gtgtcgtatt atccgtattg gcacggttca atgaatgaac
     6781 ttcaaaccaa tatgaatgcg atcagccaac ggtatggtaa agaagtgatc gtagttgaaa
     6841 cagcttatgg gcatacaaca gcgaatgccg atactatgcc aaatgctttt ggtgaagccg
     6901 aagcagctgc ggggggatat gaaccaacac cgacaggaca agcagagtat ttgctggatc
     6961 ttgccgatcg gattcaggca gtaccgaata atcgcggggc gggcttcttc tattgggaac
     7021 ctctttggta caacggaaat gtgagttggg caacacaagc aggtatgaat tatctcagtg
     7081 ttcaatccac tatgggaaac gaatgggaca accaagcgat gtttgatttt gctggaaacg
     7141 ccttgccaag cttgcgtgcg tttaaacaag caggggcgca aataaatcta gttaaaaatg
     7201 caagtttcga aagcgatgga ccaacgacca gtccttccaa ttggcaaacc tggtttcaac
     7261 aaggaaccgc taagggtacg atcaaaacgg aagcgggcac tttttttggg gactacaaac
     7321 taactttttg gcaggatcaa gcatacgagg catcagctta tcaaaatgtt agtggcataa
     7381 gcaaaggaac ttacacattg agtgcctatg taatgtctgg gggagagttt gatactaatc
     7441 agttatatat caaaaattat ggtggagcgg aactaaatac tgcggttgtt aaaacaagtg
     7501 aatggacaaa aattacaatc aaagacattc caattacaac aggtgtttgt gaaattgggg
     7561 tttatagcaa ggcttcagga aatgcttgga cgaacgttga ttttgtttca ttggtgaaac
     7621 aatagactgc ggatcgtttc gattcaagat cggaggttcc cttcagccac atcgttagcg
     7681 tagaaggcag gaatcgaacc tgcacctcgc ctttgccaaa aataaccaga aacgatttcc
     7741 agtcattttt accaaaacga gtaaagcacc agacttcatc tacactttgt attatgctaa
     7801 agagagttca acgatttcgt aagaagcaat gaaaaaattg caaaaagttg tgcagataac
     7861 ataaagtttg tttaagaaag ggtaacctta gaattccgtc gtgaatatga tttgagcact
     7921 tagatggata gaaaaaagtt ctcggaaaac agttcgtttt tcgagaactt ctttttatga
     7981 taagaatgaa acaaattaac ttatattata gtctcttttg gtagggaacc atcttatttt
     8041 taatgaaaat caagcatttt agctattgaa gaatgttgat ttatagggat tccccctcca
     8101 tattatgttg gctctttatt cccactccac tgttgctggt ggtttagaag tgatgtcata
     8161 cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt tttgcaagac
     8221 ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta cagcacggat
     8281 accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc ggatccctgg
     8341 aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga tttcttcacg
     8401 caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt cgccaagaac
     8461 acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat caggcatgcc
     8521 taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa
     8581 ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga tcgtctgtgc
     8641 tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta ggaaagagac
     8701 gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga attcattccc
     8761 aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta agaagcgatc
     8821 tcttgcattg actttgatga tgttcaagcc gaattttccg cctagacttt ccattacttg
     8881 ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca attggtcacc
     8941 gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg aaagaccaag
     9001 caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat cgataaagtt
     9061 atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc gcagcaattc
     9121 gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa aattacgtgt
     9181 tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt caaagccttc
     9241 aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt ttggtgtttc
     9301 tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat attcgcggtt
     9361 tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac catagcaaat
     9421 ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg cattctcatc
     9481 atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg cttttacttc
     9541 ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac gaatacgtct
     9601 tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt caacgttcgt
     9661 catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta tctcaaaata
     9721 gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg atatgatttt
     9781 cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat tcctgtaaat
     9841 ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct tcacgatctc
     9901 cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc agtaacgcac
     9961 caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca tagacagaaa
    10021 aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc gtattgatcc
    10081 cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga atgatatcat
    10141 acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt acacggttca
    10201 agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg tccatgattc
    10261 cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc agctgaacgt
    10321 ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt gtactttttc
    10381 caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg tatttatgaa
    10441 gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga taaatcagat
    10501 gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca ttcaaacttt
    10561 tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa aattctcgcc
    10621 attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc tcctgcgttc
    10681 tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact taagagagat
    10741 tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt cttgacttgc
    10801 gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga tggaagacat
    10861 gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac aatgggcttt
    10921 tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt cacgtgaaac
    10981 agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt tacctgatga
    11041 aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt tggcatttgc
    11101 gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc tagcgcaagg
    11161 aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata tctatgaagc
    11221 agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa
    11281 agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg gtacactgac
    11341 aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt
    11401 tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa gtgtgaaaga
    11461 ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt
    11521 acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa
    11581 aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag atacagaact
    11641 tgtatagaag actgccaagt cggtagataa aggaggtttt tatcaaaaaa catactttcc
    11701 ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt agatagaagg
    11761 gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc gtatcagtaa
    11821 caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag acaagaaaaa
    11881 aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct tcaaatgtga
    11941 tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat ttcttcttct
    12001 ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg atcatgttat
    12061 gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt tcccaccgct
    12121 cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat gcattagaca
    12181 gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat cgtcgggata
    12241 acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg ctgtttattg
    12301 aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat caattagaag
    12361 aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt aatgggaaat
    12421 ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa aaattactca
    12481 aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc ggatgctatc
    12541 aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata ggattcgatg
    12601 gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag ccaagttttg
    12661 atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct caaagaaaag
    12721 ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa
    12781 agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt tagtgctttt
    12841 tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat aatgtataat
    12901 accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg aaaaaaagag
    12961 ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact atacgaatgg
    13021 aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc aagacatgac
    13081 aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg tcatcgattt
    13141 gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg gtgacacgac
    13201 cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta aaatgtctgg
    13261 acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc ccggatttca
    13321 gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag tagcggtaat
    13381 tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc gtgacgtcac
    13441 agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc
    13501 aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca tgaagaacat
    13561 cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg ctaaccgaga
    13621 aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg gaaatagtct
    13681 agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc tagtagaaat
    13741 gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga caaccgatga
    13801 agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa aattcaaaga
    13861 aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac gaattttgac
    13921 tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc
    13981 aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca aagaagaaga
    14041 tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg
    14101 tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca ttgaattatt
    14161 atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc atgacatcat
    14221 cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa aagaaatgga
    14281 actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag aagctgtgat
    14341 gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca atccagcatg
    14401 ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta cagtaatcgg
    14461 gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag atgccatcaa
    14521 taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat caaaggatga
    14581 tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt
    14641 caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag cttgcgaaat
    14701 agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa caggcggagc
    14761 aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc
    14821 ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg gtgctacaag
    14881 aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag gctattaatt
    14941 ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac agctattgaa
    15001 gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg tctcgtaacg
    15061 gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg attaacgaat
    15121 tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac atttacgcat
    15181 ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg tcgacaagta
    15241 atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa tggtgtgacg
    15301 aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga tctataactc
    15361 atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga ggctttacag
    15421 aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca gggttatttt
    15481 taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc tcatcagaca
    15541 caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca ttcaaccagt
    15601 cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga ggaaatgatg
    15661 gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt gaccaagcat
    15721 tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca gcgatccaag
    15781 aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc atcgatggat
    15841 tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt gcaggtgttg
    15901 cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta aaaggtgaag
    15961 taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg aaagaattag
    16021 gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca gataaaggac
    16081 gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat gcttcaggcg
    16141 gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag
    16201 tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg
    16261 aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta gtagaagatt
    16321 tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt tatggattga
    16381 aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa aaagcagttg
    16441 atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact cctgaagaaa
    16501 actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg gtttgattag
    16561 aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg gaacaactcc
    16621 ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt agtcaatcgt
    16681 tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata
    16741 gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa catcaattta
    16801 caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg caaatctaca
    16861 ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt gatgcgtggg
    16921 aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg gatggtccat
    16981 cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt gggaagcgag
    17041 cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca aaaagtttct
    17101 gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc agttggaatg
    17161 gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact gatttttgat
    17221 gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat catgaaggaa
    17281 ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga aatcaaagca
    17341 gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa
    17401 gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc attcaaaacg
    17461 atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa
    17521 gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta
    17581 cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc tttgactggt
    17641 ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac taataaaaaa
    17701 ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca taaatacggg
    17761 ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca tcaaaaacct
    17821 tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag agaattgatc
    17881 gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt atcaggcgga
    17941 aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt
    18001 gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa acgtctgatc
    18061 gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga agaaatttta
    18121 aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat cgttgatccg
    18181 aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt agaagaagca
    18241 cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa aattccgtaa
    18301 tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga tattgatggc
    18361 tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag cccttggttc
    18421 gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta cagcattagg
    18481 tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac aagcactttg
    18541 cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa aagttgtttt
    18601 gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg ctgttccagg
    18661 tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga tgaactatat
    18721 cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct atcgctcaag
    18781 tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat ggttaacaca
    18841 agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg cgcttatttt
    18901 cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg ttggattgaa
    18961 tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt tgtcgatggt
    19021 catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa
    19081 cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg tttccttgct
    19141 tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta ttttgaagat
    19201 cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca atgtatcgat
    19261 tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt
    19321 cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac caacgaatca
    19381 aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt gatccttact
    19441 tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac tttttctgaa
    19501 cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc ttttagttcc
    19561 atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg gatcgctatt
    19621 ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc aacagtcagt
    19681 ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc tgctttaggt
    19741 gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac gcagaatctg
    19801 gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat tttctttaaa
    19861 aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc
    19921 ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca agctgccgac
    19981 acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc tggcttgtta
    20041 ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc agctggtacg
    20101 atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg gaatccttta
    20161 ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat cattggcgca
    20221 caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc atatttgctg
    20281 acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc aaacgggaag
    20341 aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac gggaagtgtt
    20401 tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa ggaataatca
    20461 tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa gcaccagatg
    20521 ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa gaagcaggat
    20581 tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg
    20641 aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct gtagggaaag
    20701 atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca tttcgtgtgt
    20761 ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct ggccgtaaag
    20821 tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt gaacaccaaa
    20881 tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag atcgcggcac
    20941 atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt cgtgatataa
    21001 caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg ggtgaaccag
    21061 gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc ggacacacag
    21121 tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag atcaacgata
    21181 ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg gatggcgttg
    21241 accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc aatttagtag
    21301 attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag
    21361 aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac ttgctaatga
    21421 ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg cgagaatatg
    21481 taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct caaggatatt
    21541 attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact gaaaatggcg
    21601 aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa tgaccaagtt
    21661 aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa aagaaattga
    21721 ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg aagatgctgt
    21781 aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg ggcatgcagg
    21841 aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag ggagattcca
    21901 ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga tggccggact
    21961 aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat catttgtacc
    22021 aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc ctttgatcgg
    22081 tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt atacgcctga
    22141 ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta aagaaggcgt
    22201 atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa tgtctcggac
    22261 aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag cagctcacag
    22321 cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta tgcaagcgaa
    22381 tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt ttaaagcatt
    22441 ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt gtgcatattg
    22501 aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct ttgcgagcta
    22561 aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta cgcggtatgt
    22621 taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa
    22681 tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta aagaaattga
    22741 ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat ttagtactag
    22801 cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc
    22861 ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa gaaaagggct
    22921 atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat gatttgacag
    22981 agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca gcggctcttt
    23041 attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt agcgaccatt
    23101 tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat gaaatgatcg
    23161 aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct gccctgcatt
    23221 aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg ctggacttat
    23281 tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct gccgtttaac
    23341 tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact
    23401 tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact gtgacaaatc
    23461 gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc aactgttaag
    23521 tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc catgatgtct
    23581 tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc atgtgcagct
    23641 actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca gaatggcaaa
    23701 gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt gtctaacatt
    23761 gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa tggaggaaga
    23821 gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc agctgtttct
    23881 tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca tgatttgatt
    23941 tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt gattgcacga
    24001 gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat tttgcgtccg
    24061 ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca accagtaaat
    24121 tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt tggcatgttt
    24181 agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt tattgtacaa
    24241 ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa acaagtagag
    24301 aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta gatgcataat
    24361 tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac tgaaacaccg
    24421 cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg tcggattttt
    24481 ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt acgagcttca
    24541 tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc taggttttcc
    24601 attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc aagactaatc
    24661 aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg cgtagatttg
    24721 cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc agcgatcact
    24781 gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc atggaaaatt
    24841 tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac gtcatttatg
    24901 gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg attttctaac
    24961 tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt ttcgcaaatt
    25021 ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg acggattgcg
    25081 tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat gtttaggaaa
    25141 agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct ttctggctgt
    25201 tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt taagacttct
    25261 ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat atgaagtagc
    25321 tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag tttatcgact
    25381 aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac tattttctcg
    25441 aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat ttcatcagca
    25501 ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg tattcctaat
    25561 ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg taaaccttct
    25621 tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc ggtcccgaga
    25681 cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa ttgttttaaa
    25741 ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac tattttagcg
    25801 tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt caaaaaaaat
    25861 tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg ctttatctac
    25921 aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa ggatgcagac
    25981 attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac tagacgcaaa
    26041 tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt aaacctcgta
    26101 aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac agctttaaga
    26161 aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt gtgggtacaa
    26221 tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt ttgtcaaact
    26281 taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa cacagtgttg
    26341 tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat atcgttcgcg
    26401 gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa tacggaacta
    26461 aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta ctatatagaa
    26521 gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta aaggtcctgt
    26581 tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa ctcgcttgat
    26641 caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct ataatgcctt
    26701 tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac aagcaatgaa
    26761 aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa actaccaagt
    26821 accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg tagtaaacta
    26881 cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa tcatggatgc
    26941 tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa tggctgatgc
    27001 caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg gcttttataa
    27061 gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag gagaaacaag
    27121 gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca tggcccacat
    27181 cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta aaatccataa
    27241 aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag aacaagaacg
    27301 tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc gaatcaacat
    27361 catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat tgcgtgtact
    27421 tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa ctgaaacagt
    27481 ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca aaatggacaa
    27541 gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc aagcaaacgc
    27601 tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta tcgaccttgt
    27661 aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag aaacagaaat
    27721 tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg aagctgtagc
    27781 tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca ctgaagctga
    27841 gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag tactatgtgg
    27901 ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg attacttgcc
    27961 atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg aagaagtaga
    28021 acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta tgactgaccc
    28081 attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat caggttcata
    28141 cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac agatgcatgc
    28201 caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg ctgttggttt
    28261 gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta ttcttgaatc
    28321 catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa aagctgacca
    28381 agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt tccgtgctga
    28441 aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc acttagatat
    28501 catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg cgcctcaagt
    28561 atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg tacgtcagtc
    28621 tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg aagaaggtgc
    28681 tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat acattccagc
    28741 tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct atccattagt
    28801 agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa atgaaactgc
    28861 cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta atccagtaat
    28921 ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag gtgatgttat
    28981 gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg ctggcggcca
    29041 acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta caactcttcg
    29101 ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg aagatgtacc
    29161 aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat aatttcttaa
    29221 aaccggatag tcctgcaaag agattatcca aagagactat atttttaata tagtttcgtg
    29281 taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat atacctatca
    29341 gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa aagaaaaatt
    29401 tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc atggtaaaac
    29461 tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc aagctatggc
    29521 ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa tctcaacagc
    29581 tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc caggacacgc
    29641 ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta tcttggtagt
    29701 ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt ctcgtcaagt
    29761 tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg acgaagaatt
    29821 actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat tccctggtga
    29881 cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg cttcatacga
    29941 agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc cagaacgtga
    30001 caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg gacgtggtac
    30061 tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag ttgaagttgt
    30121 tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt tccgtaaatt
    30181 gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg ttgcacgtga
    30241 agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc atacaaaatt
    30301 ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc cattcttcac
    30361 taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg ttgaattacc
    30421 agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg aattaatcca
    30481 cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac gtacagttgg
    30541 tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt aaagcaacat
    30601 ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga gcttttttat
    30661 ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca cgaaataaaa
    30721 gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca agaaaaagcc
    30781 ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta gtgaattgca
    30841 cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca cgcccggacg
    30901 ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt ttcaaaatag
    30961 gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa gcgtatgaac
    31021 accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga actggagcta
    31081 gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc atccgtgcga
    31141 ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa cgtcttatcg
    31201 acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac ttaccatctg
    31261 gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt gtactcatga
    31321 ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa tctggtgaat
    31381 taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg aaaacaatgg
    31441 aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa gttttatcaa
    31501 acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc ttcattcgtg
    31561 aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca gtagatgttt
    31621 tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga ttccaagggg
    31681 ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt taccatcgtc
    31741 gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa cgtcttgctg
    31801 gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt gtcgatgcag
    31861 aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct ttaattacta
    31921 tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa ggaatgccga
    31981 atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt aacgaagaaa
    32041 tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg caacgtgcgt
    32101 cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc ggcggacgta
    32161 aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc tcaccacaat
    32221 ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac aaacttccta
    32281 aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct gaaaacaact
    32341 tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc aaacaagttc
    32401 ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt aacgactttg
    32461 ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat aatgtgagtg
    32521 tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt actcaaattg
    32581 aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga tcactgaaaa
    32641 atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc gcgcaaacaa
    32701 aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa acgtgaacat
    32761 cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata caaaaaaacg
    32821 tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat tcgaagctgc
    32881 tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc gattaaaaag
    32941 tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc tgaaatcaca
    33001 acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg acgtaacaac
    33061 aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata tcgcgtgatc
    33121 gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga atatgatcca
    33181 aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc ttacatctta
    33241 gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc agatatcaaa
    33301 gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca caacattgaa
    33361 atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc tcaagtactt
    33421 ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg tatgatctta
    33481 gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt aatcaacatc
    33541 ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg tagcgtaatg
    33601 aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg acgtaaagca
    33661 ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa aaaagctaaa
    33721 tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc atgtgtcgca
    33781 cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt tgtcgatgag
    33841 catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt tatcaaaact
    33901 tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc agtttatgat
    33961 ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa attaggtgaa
    34021 tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac gaaacgctaa
    34081 tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag caactgcaaa
    34141 aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca gaggtaaaag
    34201 tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg gaatcattga
    34261 aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag atgttgaaaa
    34321 cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt tccgtcctcg
    34381 tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg tagtcgtatc
    34441 agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga atgcgtgtag
    34501 gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct gagttcttac
    34561 acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct gctgtatcaa
    34621 cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca gctaaaccag
    34681 gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa ttaaacaaat
    34741 taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat ctagatgcaa
    34801 aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc cgtcgcgctc
    34861 aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa actcaagtat
    34921 ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa ggaactgttc
    34981 ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat acaacatacg
    35041 gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact aaaaaaaaca
    35101 ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc gtcgtgaatt
    35161 ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg gtgaatacgg
    35221 tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg ctcgtatcgc
    35281 aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc ctcacaaatc
    35341 ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac ctgaaggatg
    35401 ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc ctgaagaagt
    35461 agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta agatcgtaaa
    35521 acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa ttaaccactg
    35581 ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt agattccaat
    35641 tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa tcgattgcac
    35701 gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc attaagcgta
    35761 tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca gacaaaatgg
    35821 ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat ggcaaacgca
    35881 tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa gttggcgaca
    35941 tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt ttgttggaag
    36001 tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga ttgaatgcaa
    36061 aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa gtcgctgata
    36121 actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt cgtaaaactg
    36181 ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt ggggttgtca
    36241 aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct cgtcgtacag
    36301 acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat gataaaagcc
    36361 ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac aacttcatga
    36421 agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa aggaggtgcg
    36481 aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga aaagacaaaa
    36541 acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt gtcgaaggtg
    36601 taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt ggaattgttg
    36661 agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact ggtgtagctg
    36721 gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa aaaaccggtg
    36781 aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac cgcctaaaag
    36841 aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat agctctgtta
    36901 tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat gcggtatcaa
    36961 acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt caaaaaccaa
    37021 tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga atgccaattg
    37081 gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa ttggtttctg
    37141 tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt gacggtcgtg
    37201 gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat tatgatttag
    37261 tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct gatgaagaat
    37321 ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag gaggcgaact
    37381 acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt tcaacacaag
    37441 cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag ttccatcttt
    37501 gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg aagaaagcta
    37561 gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca tgacagatcc
    37621 aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg agtctttaga
    37681 agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg aaggtttcgt
    37741 tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt tcctaaaatt
    37801 tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac caggtttgcg
    37861 tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta tcgcaatcat
    37921 ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata tcggcggcga
    37981 agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt taagtgagcc
    38041 gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa gacggaaaca
    38101 acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca gatatcaaaa
    38161 tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa gaaatgaaaa
    38221 caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc agcgaaggtt
    38281 tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa ggatcaaaac
    38341 ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct ggagtaactg
    38401 ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa gaagttggcg
    38461 aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt aaaggtattc
    38521 gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa tcgtttagaa
    38581 aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac cttcaaaaaa
    38641 atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg cttgaaatag
    38701 tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa gaacaaaaca
    38761 cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga gcgcccacgc
    38821 ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga cgtagcgggt
    38881 gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac aaaaacagaa
    38941 gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg cattaaagta
    39001 gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt agctgaagct
    39061 gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg gtttatatcg
    39121 atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta acaaaagttg
    39181 ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac aaaaacggac
    39241 acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt aaagcaattg
    39301 aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc ccacacgaag
    39361 taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa ggttctgggg
    39421 ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca gatatcactt
    39481 ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt gaaggtttga
    39541 aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt gaagaattaa
    39601 tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac gcagtattat
    39661 cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa agattaacag
    39721 ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg tttctcacct
    39781 agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa ggaggtgcca
    39841 aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta cgtaaccgtg
    39901 ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa aaaggacaaa
    39961 aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca ccattgttcc
    40021 gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca gtcattaatc
    40081 ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct ttagttgaag
    40141 ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg gaactaaaca
    40201 agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca attgaagcgg
    40261 ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt ttaaagtaaa
    40321 agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc gcctaggtgc
    40381 acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt ctttgccatt
    40441 tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca ttttctcaat
    40501 gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa tggacatcgt
    40561 gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac tgaatcaagc
    40621 gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca ctgctggatt
    40681 caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt acgtaacgat
    40741 cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac agatcacaga
    40801 aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt ctagattgcc
    40861 tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt cagacatatg
    40921 gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg tgacattcgt
    40981 aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac gcgtagctgg
    41041 cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta ttccagttat
    41101 ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga gcggtagatt
    41161 cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata cagcacctgg
    41221 tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct ttgttcaagt
    41281 aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac caagcgtacg
    41341 ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa gtacagttgg
    41401 tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg tttgggatct
    41461 tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg tagctttaga
    41521 aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat tcattgagta
    41581 agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta ggagggaaaa
    41641 caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa gctgaaaaaa
    41701 tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc gcagcgatgc
    41761 aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt gcattagttc
    41821 ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat acagagaaag
    41881 gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg gacgcaatgc
    41941 taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt gaagaaatct
    42001 tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact taccataaaa
    42061 ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat gaattctatc
    42121 aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac atcaaaaata
    42181 gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt gatggtgctc
    42241 gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag cacgattgcc
    42301 cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat gatagaatat
    42361 gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt gaggtgggaa
    42421 ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga ggtactgtgc
    42481 gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt gccgaatgca
    42541 atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc aggtaaaatt
    42601 cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt gtctccatac
    42661 gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt aaaactacag
    42721 gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat tgtaaagtaa
    42781 ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat aaacaacgtc
    42841 aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata ttcctcgtga
    42901 taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta ctgctaaaaa
    42961 gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa cgaatgaaca
    43021 aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc ttcgtcgtga
    43081 agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa tccgtcatcg
    43141 ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc gtaaaggccc
    43201 agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt aaaacttcat
    43261 ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag caggtattgc
    43321 acatatccat tctacattca ataacactat catcatgatc actgatactc acggaaatgc
    43381 attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat caactccttt
    43441 tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg gattaaagac
    43501 tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc gttcattaca
    43561 agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc ataatggtgc
    43621 ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt ttatcagtaa
    43681 cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg attgagttcg
    43741 aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc atcgttgaac
    43801 ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt ttattatctt
    43861 ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat gaattctcta
    43921 ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa ggcttagcgc
    43981 tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga cctgctacag
    44041 taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa gatatgtaca
    44101 tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa cctggacgtg
    44161 gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt cttccagtcg
    44221 attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca cgtgttggac
    44281 accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca attgaaccgt
    44341 tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt tttgtgaact
    44401 taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca caaaaagaaa
    44461 aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac aattgtttga
    44521 aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct gaaatgatca
    44581 aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg cacgacttag
    44641 gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc aacgtgagtt
    44701 atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat ttaacaactg
    44761 atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa gttcgctcaa
    44821 ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt cgtcaagctg
    44881 cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac atcgtcattg
    44941 aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa cgtcaaggcg
    45001 gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca atggttgtct
    45061 tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt ttagagtgtt
    45121 atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag cgaatatcgc
    45181 ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc tgtcatgaca
    45241 gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa ttttgtcttt
    45301 ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga gccaattatt
    45361 gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc attgaaagat
    45421 gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa tggttcagga
    45481 aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg gatcgtgaaa
    45541 gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat ggttggaatg
    45601 gtttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga cgttgctttt
    45661 ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa agatgcactt
    45721 gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc aggagggcaa
    45781 aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat tattttagat
    45841 gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac gatcaagaag
    45901 atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga tgaagcagct
    45961 aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg tacaccaaaa
    46021 gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc atttcctgaa
    46081 aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata tatgacagaa
    46141 gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc acttatcagc
    46201 cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc aaagaaaaca
    46261 gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt cagcatttga
    46321 atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat attcagccgg
    46381 atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc tttcagtttc
    46441 cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt cctaaaaact
    46501 ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa caagttgggc
    46561 tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa atgcgtagag
    46621 tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat gaaccaacgg
    46681 caggacttga tccccagggt agaaaagaaa tgatggagat gttttggcgt cttcataagg
    46741 aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc aattttgcag
    46801 attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg caagaggtct
    46861 ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc accgagtttg
    46921 cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca gctgaagaat
    46981 tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa attgatattt
    47041 ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc taaattatta
    47101 gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac gtaccttttg
    47161 ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa attttttatc
    47221 cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca ggtcttgttt
    47281 acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga atttggtgtg
    47341 atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc gactctactc
    47401 acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct tcgtccattg
    47461 aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc tcttcgtttt
    47521 gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc tcgcggtgtt
    47581 gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt gctgattcct
    47641 ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga agcaagaggc
    47701 tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa tagagacacg
    47761 atggttatca ttgcttttgt attattaact gtgctactga tctttttgag aggataaacg
    47821 ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag gatgcgagca
    47881 gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct ctttctttta
    47941 tctgaaaaga aagaacgaaa aaattttata aaaaaatcat tattccagat aaccttttag
    48001 gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcatacg acggaacaaa
    48061 ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag tagaaaaaac
    48121 attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa ggacagatgc
    48181 cggagtacat gcgattgggc aagtaatcca ttttgattat ccagaggagc gtccgttaga
    48241 acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa gacaagtaga
    48301 aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt atcaatttcg
    48361 agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt attttcctta
    48421 ccctcttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga ctcacgattt
    48481 cacctctttt tgtgcatcag gaagctcgat agaggataaa gtacgtacga tctacgaggc
    48541 taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg gaaatggctt
    48601 cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca atggccgatt
    48661 gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag caggacctac
    48721 agcacatcct gaaggacttt atttgtatga agtgaaatat gaataagagg ttgtgacaga
    48781 agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat tacttctcaa aattttgcaa
    48841 aatttcggct tattttcgaa ggagctactt ctggaacacc gtgaatcaga ggttgtgaaa
    48901 aagctgccct gcatcaagta ataagaacaa ccaaacaaaa atagctcgtc atatttttcg
    48961 tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt
    49021 gaaatcagcg ttttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    49081 tgccgtttgt tgagctaata ccgaaggtca gctgattgaa caccgtttaa gaaagaggtc
    49141 gtgagcctgt catttaactc tgagtcacaa gtaacaattt caaaaaacag cttctcatct
    49201 ttgatgaata tcgtgacttg tggacgaggc gttgataagt gaatcatctt tttgttacct
    49261 attttttctg taaccaaaaa acgatggaac gttgttttga ttgcccttaa tgaggacagt
    49321 cggaacaggt ttgtcatttt tatatttaaa tggcacaaat ttttctaatt atttgtcatt
    49381 tagcgctgaa aatttttgct atttatctaa aaaagtgata actacttaag atgaagcaga
    49441 atatttacgc catacggcat acaaccgcga aaaatataaa aaaaagaaaa cgattgaacg
    49501 cgtttttgca gtcgcaaagg aaaagcatgg aatgcgctac atcaagtatc gagacttgga
    49561 aaacaatcag gtgtatacgc tgattgtttt tgctgcaatg aatctgaaaa agttgtccac
    49621 ttggcagttg gaacatatgt cttcatatct tttattatac aaaaatagca aaagcaccag
    49681 tctacactct tagtaaagag gaaactgatc cttttgtcta cattcttaaa gtacttaaac
    49741 taagtacttt attttactct ttaggattga tacagtagca tctatccctt tattaataat
    49801 cttttatttt tttaaataaa gatgttgtct ccatcttcag gcactaaaac tttttcggaa
    49861 attccttttt ctttattgaa agactttaat tcttctcttg acagtcccca atggtttaca
    49921 gcttccatat gaacggcaat aattgtggaa tctggtattg ctttatgcaa ttcatataaa
    49981 tcgtctttcc ccataataag agattctcca actaaaaaat gattatctcc agcatttgct
    50041 actatgattt ctggattata ctgatcaata gtttgtttaa cagcttcata ccaaactgta
    50101 tctcctgtaa agtatagtgt cttctcagtc gaatgtttga aaacaatacc gcaaacatta
    50161 cctacataat tcaagatttc tcctctgcca tgtttgcctt ttgttttaac caattgtata
    50221 gattcaaaaa ctgtgtcttc tcgtagtacc tccacatttt taaaaccata tagtcgaatt
    50281 tcattcgcat cattttcatc ttgcacaaat aattttattt ctttaggtag gacctctttc
    50341 gctatatcgt cccaatgatc tagatgtaaa tgtgttacaa taacagcatc aataccgttg
    50401 ataatatctt cgactggcat tggcaaatca actagaggat tcatttgatc ttttcttggt
    50461 gatggaaaag gacctttcga aataaatggt ggaagtgttc ctttttccgc taacattgga
    50521 tcaattaaaa attttttacc tccatagtct aagatagctg ttgcattacg aatgtgatgt
    50581 atattcataa acgttgtctc ctttttgttg atatgaaata tttttagtat aattcaattc
    50641 catacaataa ataaaccgaa caaaaaaagt cttttactat ttttacttaa tttatgaaag
    50701 gttgtttacc atgttagacc aaacagatat aaatatcatt gaggaactat caaaaaatag
    50761 tcgaatcaca atgaaagaac ttggagaaaa agtacatttg actggacccg ctgtctcggc
    50821 gagagtgaca aaactagagg aaagtggagt tattgaaaat tatacagtaa aattaaatat
    50881 ggaaaagcta gggttttcga ttcacgcttt tttaattata attacgcaaa atttaaatca
    50941 taaaccctat ttatcattta ttaaaaatca tgaaaaattc ttgattagaa attataagat
    51001 tagtggaggg ggctgttatc ttttggaatg tagatttact tcaaaaaaat caatgaacag
    51061 ctttttggaa cagcttaatg aatatgcgaa ttataaatta tctattgtta tagattaata
    51121 ataaaactag cagatggtaa aaatactaat atgcgagaca cacattcaac cataactata
    51181 taacttatca a
//
LOCUS       DAZUMT010000006        50000 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000006 DAZUMT010000000
VERSION     DAZUMT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.006"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..1722)
                     /locus_tag="ACG8FM_000329"
     CDS             complement(22..1722)
                     /locus_tag="ACG8FM_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093022.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2229..3389
                     /locus_tag="ACG8FM_000330"
     CDS             2229..3389
                     /locus_tag="ACG8FM_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5093023.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3405..3848
                     /locus_tag="ACG8FM_000331"
     CDS             3405..3848
                     /locus_tag="ACG8FM_000331"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5093024.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4331..4861)
                     /locus_tag="ACG8FM_000332"
     CDS             complement(4331..4861)
                     /locus_tag="ACG8FM_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS5093025.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4966..5517
                     /locus_tag="ACG8FM_000333"
     CDS             4966..5517
                     /locus_tag="ACG8FM_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5093026.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5670..6143)
                     /locus_tag="ACG8FM_000334"
     CDS             complement(5670..6143)
                     /locus_tag="ACG8FM_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5093027.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6281..7753
                     /locus_tag="ACG8FM_000335"
     CDS             6281..7753
                     /locus_tag="ACG8FM_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5093028.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7750..8460
                     /locus_tag="ACG8FM_000336"
     CDS             7750..8460
                     /locus_tag="ACG8FM_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS5093029.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8812..9096
                     /locus_tag="ACG8FM_000337"
     CDS             8812..9096
                     /locus_tag="ACG8FM_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS5093030.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9413..9796)
                     /locus_tag="ACG8FM_000338"
     CDS             complement(9413..9796)
                     /locus_tag="ACG8FM_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS5093031.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(9798..10382)
                     /locus_tag="ACG8FM_000339"
     CDS             complement(9798..10382)
                     /locus_tag="ACG8FM_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS5093032.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10429..11904)
                     /locus_tag="ACG8FM_000340"
     CDS             complement(10429..11904)
                     /locus_tag="ACG8FM_000340"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS5093033.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12085..12396
                     /locus_tag="ACG8FM_000341"
     CDS             12085..12396
                     /locus_tag="ACG8FM_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093034.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12511..14847)
                     /locus_tag="ACG8FM_000342"
     CDS             complement(12511..14847)
                     /locus_tag="ACG8FM_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS5093035.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            14989..15657
                     /locus_tag="ACG8FM_000343"
     CDS             14989..15657
                     /locus_tag="ACG8FM_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5093036.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15660..16703
                     /locus_tag="ACG8FM_000344"
     CDS             15660..16703
                     /locus_tag="ACG8FM_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5093037.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16940..17821)
                     /locus_tag="ACG8FM_000345"
     CDS             complement(16940..17821)
                     /locus_tag="ACG8FM_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5093038.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            17980..18708
                     /locus_tag="ACG8FM_000346"
     CDS             17980..18708
                     /locus_tag="ACG8FM_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS5093039.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18864..19181
                     /locus_tag="ACG8FM_000347"
     CDS             18864..19181
                     /locus_tag="ACG8FM_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5093040.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19174..19782
                     /locus_tag="ACG8FM_000348"
     CDS             19174..19782
                     /locus_tag="ACG8FM_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS5093041.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19837..20628
                     /locus_tag="ACG8FM_000349"
     CDS             19837..20628
                     /locus_tag="ACG8FM_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS5093042.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20766..21254
                     /locus_tag="ACG8FM_000350"
     CDS             20766..21254
                     /locus_tag="ACG8FM_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5093043.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            21305..22177
                     /locus_tag="ACG8FM_000351"
     CDS             21305..22177
                     /locus_tag="ACG8FM_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5093044.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22353..22715
                     /locus_tag="ACG8FM_000352"
     CDS             22353..22715
                     /locus_tag="ACG8FM_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5093045.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22724..23476
                     /locus_tag="ACG8FM_000353"
     CDS             22724..23476
                     /locus_tag="ACG8FM_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093046.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23490..25292
                     /locus_tag="ACG8FM_000354"
     CDS             23490..25292
                     /locus_tag="ACG8FM_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093047.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25569..26609
                     /locus_tag="ACG8FM_000355"
     CDS             25569..26609
                     /locus_tag="ACG8FM_000355"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5093048.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26661..28805)
                     /locus_tag="ACG8FM_000356"
     CDS             complement(26661..28805)
                     /locus_tag="ACG8FM_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5093049.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(29162..30535)
                     /locus_tag="ACG8FM_000357"
     CDS             complement(29162..30535)
                     /locus_tag="ACG8FM_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5093050.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACG8FM_000358"
     CDS             complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACG8FM_000358"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS5093051.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACG8FM_000359"
     CDS             complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACG8FM_000359"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS5093052.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(31983..32996)
                     /locus_tag="ACG8FM_000360"
     CDS             complement(31983..32996)
                     /locus_tag="ACG8FM_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS5093053.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(33077..33676)
                     /locus_tag="ACG8FM_000361"
     CDS             complement(33077..33676)
                     /locus_tag="ACG8FM_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5093054.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            33862..34563
                     /locus_tag="ACG8FM_000362"
     CDS             33862..34563
                     /locus_tag="ACG8FM_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093055.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            34573..37284
                     /locus_tag="ACG8FM_000363"
     CDS             34573..37284
                     /locus_tag="ACG8FM_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS5093056.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            37735..39363
                     /gene="treC"
                     /locus_tag="ACG8FM_000364"
     CDS             37735..39363
                     /gene="treC"
                     /locus_tag="ACG8FM_000364"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS5093057.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(39423..40604)
                     /locus_tag="ACG8FM_000365"
     CDS             complement(39423..40604)
                     /locus_tag="ACG8FM_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5093058.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            40856..42379
                     /locus_tag="ACG8FM_000366"
     CDS             40856..42379
                     /locus_tag="ACG8FM_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5093059.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(42448..43176)
                     /locus_tag="ACG8FM_000367"
     CDS             complement(42448..43176)
                     /locus_tag="ACG8FM_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5093060.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREATLANGETIPASIRGLKKLIS"
     gene            complement(43189..44469)
                     /locus_tag="ACG8FM_000368"
     CDS             complement(43189..44469)
                     /locus_tag="ACG8FM_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5093061.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(44610..45212)
                     /locus_tag="ACG8FM_000369"
     CDS             complement(44610..45212)
                     /locus_tag="ACG8FM_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5093062.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            45457..45663
                     /locus_tag="ACG8FM_000370"
     CDS             45457..45663
                     /locus_tag="ACG8FM_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093063.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            45975..46694
                     /locus_tag="ACG8FM_000371"
     CDS             45975..46694
                     /locus_tag="ACG8FM_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5093064.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(46775..47458)
                     /locus_tag="ACG8FM_000372"
     CDS             complement(46775..47458)
                     /locus_tag="ACG8FM_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS5093065.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            47677..49863
                     /locus_tag="ACG8FM_000373"
     CDS             47677..49863
                     /locus_tag="ACG8FM_000373"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS5093066.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        16261 a         8849 c         9976 g        14914 t
ORIGIN      
        1 ggatgtattt atcccttttg cctaagcacg taaaagcttg tatctatgat aaataaataa
       61 acatagcaag gtaaaccagc caatcaaagt aagagaaaga atgaaataga aatagattgg
      121 cgtttgataa tgaagcgtga gctcgttgat tcctttttgc tgcttcacga ttggggtccc
      181 gatatctgtt acttcataat cagtcaatgt ttttccattt tgctgtagca cagtccgatc
      241 gtagacgata acagggatat gaacagtttt gtccccagtg tttttccact tgattgtcaa
      301 aagaccgcct ggatgtgttt ttataaaagt tgtttcgttt tctagtattt cttcgttata
      361 ccggtcgtat ttattgtctt ttgtctcctt atagattggc agatagtcag gtgtggtttt
      421 ttggaaaacg aacaagctgg tagacatatc tttgctgaag aatgttttcc ttgcttcttc
      481 cggcgtatcg aatagataag tatgtcgact gacaaatgtt tcattttccc acttttccag
      541 atgctggtat aagttctgga tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa
      601 aagacggaac cacttttgtt cagaccactg gttcagtgaa agggcaaggt aaagtagaag
      661 caatagcgta aatggcacga aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac
      721 atttttttcg atcaatgtcg tccatggaat caagctagta gaaagaaaaa agaaaacaag
      781 acttgtgtac aacacgattt tttggaataa ggtgttcttt tgttgaaaga aaataaaaag
      841 caaaaaacct gataatatca atataggaaa ggccttaggg taatggagcc aataactgcc
      901 atccagatct atcgcctttt gactcatttc tctgttaaca aagggaggaa gaatcttatt
      961 cccactgtaa acaatagcta atcctgccca aatatttgct gtcagaacaa aataaagcaa
     1021 gaccgactta atcagttgaa gcagtgcttt tcctttttca gtctggttaa aaaagtaata
     1081 aagataaaaa gggatataca tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt
     1141 cataagtccc actgagacag cgactttgat aacagggaac tttttatgaa ggacgaaatc
     1201 agcaataggg atcatacata aaggataaaa acaagtcccc cagctagtga atccttggtt
     1261 gatagaccag tattgaatag aatacgtact catgaataaa acggctactg gtacacttac
     1321 ccaatttcga acattgacat atctcagtaa aatcagcata gaaaatcctg aaataagata
     1381 aagaatgaaa tttgaaagaa tctgataatg aaaccagcta cctgcaagca acacgatcaa
     1441 tccttgtaaa taagcgaaaa atgggccgta aacggcattc acgatcctgc cactttgctg
     1501 gaatccataa agggaaggaa aataatggaa atccccatat ttaatttgtt gcgcggcatc
     1561 atagaaccga ttgtaatgga atggggcatc agcacccaaa attagtttat gtgtgaataa
     1621 atgaggcatg agaaatgcca aagcaatgaa aaaaattgct aaaaatgggc catatttttt
     1681 caattgtcga actcctttgc aaactttaga taaattcatc atatatccta tgcgagaggt
     1741 aggcaagaaa ttagaaaatt catgaaaata tttatgaatt ttacaagaaa aacataagtg
     1801 aaagttctaa ggagagagga tgcgacataa gttttgttgc atctccttgt tttgaaacag
     1861 aatttttctt aatactcgaa acaaagtagc tttttcatga cctctcggta aacggtgttc
     1921 aatcagctgc ttctcggtaa taagctcact agcctagaaa atatgagaag ctattttctg
     1981 actagcgctc ttattactca aagcataacg cagttttcac aacctcttcc ttaaacggtg
     2041 ttcaaaagct tgcatctgcg gtaataagcc caacccaaca aaaaatatga gtagctattt
     2101 ttttgggctg tacttattac ttgatgcagg acagcttttt cacaacctca tcggctttca
     2161 ttttctattt ttttcctgaa cccatataat aaaagaaaga acaaaaaaac ttgcaaggga
     2221 ggacacatgt gaagcagagt aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt
     2281 tattaatatg tatcgtcgta gggttactga tcgctttata tccattttat gtggattctt
     2341 taaatagttt tattgatcaa aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa
     2401 ttgaagtgca gcgaaagaaa atggaggaga aaaaccaacg attaactgac caaggattca
     2461 atcctggtac tgatcctttc aatgaaaaga acagaaatga aagtacaaca tccagccaat
     2521 tagaggaatg gctgatcgga agtatcaata tacctaagat ccaattaaat atttcacttt
     2581 atgatcggct caatggtagg atccttgaaa atggagcagg cgtcttgcaa ggaacctctt
     2641 tcccgcttgg tggaaattcg acgcattcag tgatctctgc tcacagcggt ttacctaatc
     2701 gacggttatt taccgaatta gatcgtttag aaaacggaga cacgtttatt ttgactgttt
     2761 tgggggagaa attggcttat caagttaaaa atatacaagt ggtactgcca gatgacacgt
     2821 ctgttctaac gatttcagaa ggagaagact tagttacctt gcttacatgt acgccgtata
     2881 tgatcaacac gcatcgtttg ctagtgacag gacatcggat tccttataac gaatcggtaa
     2941 aaaaagaaga aggaaaagga aatcaggaaa gaaccatgcg gcaatggttt attcttgcag
     3001 gaacgataat agctgtagtc atactgttag tgtttatcgg acgactaatc tatcagtata
     3061 gattaagtaa aaaagtgctt gatttttcat ttattattag tgattcgtca gggaaaccgg
     3121 taaatggtag ttcattcatt ttgaaacata agaaaaaaac acttactaga aatggtgtgc
     3181 cgttttcagt acaaagcgac catcatggga aagtcaagct ggatcaattg cctggtggaa
     3241 cttatcgaat cgtttcagct gatgatccta aagttgctgt ttcttttggt attagaaaag
     3301 taaaacaaga gaagatatac ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa
     3361 atggttttta ttttaaaatc aatgaataag taaggagaga aaaaatgatc cgcacaacaa
     3421 caaaattgtc tgccaaagaa ctggaatctg ttctatcgat ttggctgcat gctaacttgg
     3481 attctcatga attcattcca tcaacttatt gggagaatca agtagaatct gttaggaaag
     3541 ctttaccaga tgcagtgatt tacttagcag aaatagatca agaaattatt ggttttgctg
     3601 gggtgattga tcagtttatc gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa
     3661 tcggtacgca actaatgaaa aaaataaaag aaaccaataa aacactgact ttgacagtct
     3721 atcaaaaaaa tcaatcagct attcatttct atctttcaca aggttttaca attcaaacac
     3781 agcaaaaaga tgaagcagta aatgaggagg aacttgtgat gaaatggaat caaaacgctg
     3841 atttttgatg aacagaataa cgaaataatg ctcccagcct aattttttgc tgggagcatt
     3901 atttcatttt gaagtcacta atttaactgt gtgaatatgc agttgttaca atctttgata
     3961 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     4021 tatttttcca tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttgat
     4081 aaacggtgtt caaacggctg cttctcggta ataagcccac tagcctagaa aatatgaaaa
     4141 gctattttct aactagcgtt cttattactc aaagcataac gctgatttca caacctcttg
     4201 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaacatgagg
     4261 aactattttt ccgtttcttt ctctaatact caggtcagaa cgctgatttc acaacctctc
     4321 tttcacaacc ttattttact cgtttataat tttcatcgaa gtattttccg ctacggatat
     4381 catctggcat tccatcgtat ggttcttcgc taataacatc ttcattgtct atacctgcat
     4441 cacccatata agtgatcgta gcaaattcag gaacaagtaa ttttggataa gtttcatatt
     4501 tttcacgagc tgcttccatt tctgcgatat gttcgttttg gaaagtaaca gtgatttctg
     4561 gatgttcttc aacccattta gggaaaaaga tcattccgtg aagtttcttt tcatttggca
     4621 taaagtccaa tgccacgtct gtccctgttg gctctgtcca agtgattttg aatacgccgt
     4681 ccgtcaattt aacgatatca gcttcttggt cttttaccca acggcctttg accattccgc
     4741 catggatacg gtaatcaaca gtatggtcat ttttggcata ccattcatat tcccaaccat
     4801 tgtcatacgt atagataaag tgagttccaa taaaatcttc caattcttta aattctttca
     4861 tgtttaagca tctccttata aattaaaaca tgtaataaac tacatgtttt cgattactca
     4921 tttatgatag aatggattta aagatttgtc aaggagggaa aactgatggc aagaaaaaat
     4981 acacttcgat atattttgtt agggttatta agtaagaaga agatgacagg atatgaattg
     5041 aatcaatcgt tcaaaaatga aataggagaa ttttggcaag caaaacatag tcagatttat
     5101 cctgaacttg caaaaatgga agagcaagga atcattcagc atcaagtaga gatcacaggt
     5161 gaaaaattgg aaaagaaagt ttatgaatta acagaagaag ggaaggaaca attggcagag
     5221 tggattcata cacccaccaa tgaactaccg gttaatcgag acgaatttgt tctgaaattg
     5281 tatttcgtca aagacgtgaa tgatccggca ttagtagaaa ttattcagca gcagcgtgac
     5341 cttcatgaag aaaaacgact tcatctatta gagcgaaaaa aaacgatctt tcctactgaa
     5401 aaagaaaaac atgaaaatta cggacactac ctgattttag cgcatgcgat caatcgggaa
     5461 acagaatatg caaattggct aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa
     5521 aatatccgaa cagtgatgga gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg
     5581 gatattctta tgttcaaaca ggcctttttt caagtattta tgtagcatct acttattaaa
     5641 aagcttggta cttaggagat tagtggcgtt tagagttctt ttaacaactg ttcgtagtca
     5701 gctttttcat cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg
     5761 atgtacatct tagcattctc ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg
     5821 tcgatcaatt cgatatcttt gattgctttt gaaaaaactt tacggacatg gtgcattttc
     5881 ttttggcgga tttctgcctt ttctgcagct tttgacaaag taaatccgtc atctaaatat
     5941 tttttgatca gttctacttt tatgactgta ggtaaacgat attttctagg acaattcgca
     6001 tcagcttcga tcgaacgaat gaatcccttt tgttcccaat accttaattg acgtggagac
     6061 acacctacga tatcacttaa ttcactgatg ccaacaacca attgatcatt ttccaacaaa
     6121 cgtttaaggg aagccccaac catttttgtc actccttttg tcctcttgct tcccttaatt
     6181 atatccgtat taacttatgt gtcaagtagg ttgacaaata gcgaatttag gactaaagta
     6241 ttctagtagt ttcagaaaaa attcagaaag aaggagaaat atgagtaaca accaacccgt
     6301 agatatttac ggaaaaccat ataatcggac acttttagtt gttgtcttat taattggtac
     6361 attttgtacc gttttgaatc agaccttatt gacgacagct tttcctacat tgatgaaagc
     6421 tttcgatatt tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg
     6481 gatcatgatc ccaataacag catggctgat caataaattc agttcaagaa atttatatct
     6541 ttctgccatg acgatctttt tgatcggaac gattacttgc tttacagcac ctaattttag
     6601 tacattacta atcggtcgtt tgatccaagc ttgcggtgtg ggagtatcga tgccattatt
     6661 gcaaaatatc atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc
     6721 agggatcgtt attggacttg ctcctgcttt aggccctaca ttatctggtt atatcattga
     6781 taactaccat tggcgtgact tgtttggtat ggtactgcca atcgttgttc ttgtcttggt
     6841 tttagcattc ttcctaatga aaagtgttat tccattgtca aatcctagca ttgatatttt
     6901 atccgctatt ttatcaacga ttggattcgg tagtttactt tacggatttt ctagtgtagg
     6961 agatgacggc tggggaagct tgaaagtaat tggtttctta gttgttggtg tgattttcat
     7021 cgccttgttc tcatggcgtc aattacattt ggaacatcca ttcttggaac ttcgtgtatt
     7081 caaatcatct acattcacga ttgcagctgt tttagctggt gtaacaaata tggcgatggt
     7141 aggggcagaa atggtcgtac cattgtatat tcaaaatatt cgtggagaat cagcattcca
     7201 ttctggcttg atgttgttgc cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg
     7261 tgcgattttc gataaatacg gcgcgaaacg tttagccatc acagggatgt tcattttgac
     7321 agcggcaact gcaccatttg ctttcttgac aaaaacgact ccagttttat atatcgtgat
     7381 cctttatgcg atccgtatgt ttgggatctc catggtcatg atgccggtaa cgacttctgg
     7441 tatgaatgca ttgccaaata acttgatcag tcatgggaca gcggtaaata atacattccg
     7501 tcaaatcgca agctcgattg gtacagctat tttgatcagt gtgttgacga atgtgacaaa
     7561 agcaaatatc ccaagcaaat ctgtattgga taatacgcca ttagcttata aagatcaggc
     7621 aatcaatgcc acactgtcag gttaccatgc cgcgtttatc gtagcgatca tctttgggat
     7681 tcttggcttt attattgcct ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg
     7741 ggataatgca tgattattgg agttttgatt gtcagcttgc ttgcttttgc tttaacgaac
     7801 gtgttcgcta aaaaaacatg gcaaaccttt ttatctttga ttttcggact aatcttcgtc
     7861 gcttcactaa gtttgatcgt cgctaatttg tccaatcact ttggaatgga aaaagtaacg
     7921 gaaacaaaaa cagaaaaaat tgtttccagt gctgattccc aaggagcaga tatgcttttg
     7981 tataaagctt tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa
     8041 gaaaaaccaa aagcaactgg tacggataac gaaacaaata aagtagaaaa gacagacggc
     8101 gatgctgaaa aagtgacgaa aactacttat tgggaataca aaaacgacat gtataaattc
     8161 tggttcaata ttgctgataa caatcacgaa tatgacagcc gagtgaatac attcaaaatc
     8221 ccagagacat gggtagaatt aagtacggac caagcagcca agttagctga attagtgaag
     8281 aaacagcaaa gtacgatgga aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa
     8341 gcaatgactg aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac gaaagcctta
     8401 gctgctgaat tccaacaaca agcattcgca aaattagtga aagaagcaaa aggcgaataa
     8461 tattttgaac accatgtagg aggttgtgaa aaagctgctc tgcatcaagc agtaagaaca
     8521 atcagataaa aatagcactc catatttttc atctgattgg gcttagtgcc gtagatgcaa
     8581 gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactct gagtcacaag
     8641 gagcaatttc aaaaaacagc ttctcatgtt tgttgactat tgtgacttgt gaccgaggag
     8701 ttggcaggag aacaccgttt attcgaaaaa aggaactgcg acaagacttt tgtcacagtt
     8761 ccttcttttt tttgctatgc tatactagat gtaaaaaata ggagaaataa aatgagggaa
     8821 atttattctt ttgaatcaaa aatctatgct tccgaagtcg gcaagggcgg ggcatatatt
     8881 atttttccgt atgatataag aaaagaattc ggtgctggaa gagttaaagt ccaagcaact
     8941 tttgacaatg tgccgtatga aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt
     9001 tcagtctgct acatccttgg actacgaaaa gatattcgta aaaacatcgg aaaagaaatc
     9061 ggtgatattg tagcagttac agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt
     9121 cacaacctct tgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg
     9181 caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg aagcggcttt
     9241 atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg
     9301 gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca taacgctgat
     9361 ttcacaacct cttctttcat aatggttttt caccatcttt ataataaact ggttatttga
     9421 tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct tctgacatcg
     9481 gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg gaagtgatcg
     9541 tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct aagacatcgt
     9601 ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct gtatcacgag
     9661 aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg atcaataagt
     9721 ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt acttcaacta
     9781 tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt tacaatttca
     9841 aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt ttgaggctga
     9901 ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag
     9961 ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa ctttcgttgc
    10021 tccttcattg ttacatcagc agcacgtgca atcaggttgt cgcctaaacc aacagagata
    10081 cctgcgactg ttttcatact tgccggtacg atgatcattc catcggtcaa aaatgatccg
    10141 ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag gctttcgaat
    10201 tcagttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg actgatgatt
    10261 ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat ggcataaatg
    10321 gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc caattgttcc
    10381 attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt atttcaaata
    10441 tttttcccaa tcttctactt cttggaattt tgcacgtaca aaatgttctt tcatatcgaa
    10501 aggaactgta ccgtcaaaga tagtttttgc acttatccca cggaaacgaa tagagccagg
    10561 attatattct ggtaattctg atgggtccaa tggatggttc ctcattcctg ggatcacgat
    10621 gatatcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa
    10681 aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc
    10741 aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac ggatttgcat
    10801 gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta cttttcctgg
    10861 aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg ccatagatac
    10921 gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc ggtgagtaac
    10981 tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc caggaaattc
    11041 tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat ttggcatgat
    11101 ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg ctataccatc
    11161 aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat tgtagccaag
    11221 tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta aaccaatatt
    11281 gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg
    11341 gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa ccatacgatg
    11401 gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac ccattgtgat
    11461 gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa
    11521 gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt
    11581 cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc
    11641 atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt tcacacgtgt
    11701 gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag tcggacgttt
    11761 tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt caggatcgat
    11821 ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga tgacttttct
    11881 taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta cctatctata
    11941 gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc tacaatggaa
    12001 tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcactaa aagaaagcgc
    12061 atacttagtg ttggaggaag gaaagtgaaa aaaatggagg agtcatttcc taaagcagta
    12121 aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga agtaaaatac
    12181 gtgaaatctc attggacaga agagataacc gatgccttgc aatttggaaa aaaaggaaga
    12241 ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac tgaagtaacg
    12301 attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc agaagagttg
    12361 tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa aaacagcgcg
    12421 aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca gcgctcttat
    12481 gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc
    12541 ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata tagtagagac
    12601 gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta gatccgtcat
    12661 gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca cacctaatat
    12721 cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt
    12781 acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca cagatgtata
    12841 agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa aagcaataag
    12901 gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc cagctaaatc
    12961 agtataaatg attttatctt catctgattt gcctttattg taatcatcta gataatccaa
    13021 gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag gcaagctgtc
    13081 tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt cagtagttaa
    13141 gacatttaca ccactgtctc tttgagcttt gacgatttct gattctttgt tttcgttgac
    13201 gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat tcattgtaga
    13261 agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat tctgatacat
    13321 cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat agtaagtatt
    13381 gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc
    13441 tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat tgccgtctac
    13501 gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag aataattgtc
    13561 ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc
    13621 tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat tttgattcga
    13681 gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc ctgtagtacg
    13741 ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat cgacgtatgt
    13801 ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt
    13861 tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac gagtctggtt
    13921 agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt gcgtgttatc
    13981 gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga tgatcccaat
    14041 actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat tgttgaagga
    14101 aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt gatcatcttt
    14161 tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa attcaatgat
    14221 tcgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca ctaatttttc
    14281 tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat ctaacgctcc
    14341 ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag cgatggccac
    14401 tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat ttgtcaagcc
    14461 gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa caccacttaa
    14521 tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt tgtaactttg
    14581 aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct taaagtcttt
    14641 agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc caccaatcac
    14701 attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga attctttttg
    14761 tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga ctttataata
    14821 tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa atattcggta
    14881 tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta actgaagatg
    14941 aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat gaatcgtatt
    15001 ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt ggaacgagcg
    15061 ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact agaagcgaca
    15121 cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta tgacttgcta
    15181 gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc aaaggccgat
    15241 tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac aaaacccatc
    15301 gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa gatcaattac
    15361 agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac agtgttacaa
    15421 gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa attactgtct
    15481 tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg attagatacg
    15541 aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg ttttgaaaag
    15601 acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt tatttaaaga
    15661 tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc ttcatttcga
    15721 tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga cattctatct
    15781 cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt gcggggttca
    15841 gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg catccaatca
    15901 gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt cgaatggatg
    15961 aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt atggtccaag
    16021 aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc catgaattca
    16081 aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca aatctctctg
    16141 acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag ctttcacaat
    16201 taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga
    16261 aagagtatcg attagatgaa caaatcagag aagtaatcgt tttttttcag cctaaatggg
    16321 agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca ggcaatgaag
    16381 agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac aatcaaatca
    16441 atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg gaagtgaccg
    16501 attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa ttttatcaag
    16561 gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt aaaaaaattc
    16621 tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga acgacagcaa
    16681 tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc tattgaaggt
    16741 ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg ttcttttggc
    16801 ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca ttgaaacaag
    16861 gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg ggtggataga
    16921 tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa aatgtatctg
    16981 aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct gttgttggag
    17041 aaatagggag aatcacagta ttttctttta atagattctt gactgttttt tccataacaa
    17101 tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt gctcctttat
    17161 aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt tgatacaagt
    17221 tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga tccaatttct
    17281 ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct gtttcagtag
    17341 gcaaccactc aatcgaactt ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac
    17401 tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc aactggactg
    17461 taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta taattgatca
    17521 ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag gtttcaagca
    17581 cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc aataagcttc
    17641 cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg tctaattctg
    17701 tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt cgatcagcag
    17761 ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta tggatatcca
    17821 tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg aattggtgta
    17881 atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag aaatttttct
    17941 gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg agttttgatc
    18001 aatgtaggtt ccgttttgtt tggcggacta attggcggat tagtaggaaa taaattaagt
    18061 gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg gatgggaatc
    18121 tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt gataggaaca
    18181 gcgattggtt tgattgttca tttaggtaat gggataaata agggggctgc gctgatgcaa
    18241 gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga tgaatttatc
    18301 agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat ctatggaagt
    18361 ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt ccttgatttt
    18421 ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat agcaattccc
    18481 caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc attgactaca
    18541 ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc taccggtttt
    18601 agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat gatcgttatc
    18661 atgccactga gctggatgtg gacaaattgg attatgccac tgttataata aaaatcaaag
    18721 agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt tttgtatttg
    18781 acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat aaaatattac
    18841 acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt cttgatggta
    18901 ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca aaaatcgtac
    18961 aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga ttgaaaaaag
    19021 aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga tattactcgt
    19081 tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt gtttttcgag
    19141 attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa tgaagttgtg
    19201 gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta ttatgaggaa
    19261 tattttttag attctggaaa aacattagat cagatcattg atgaatatgg tacaccaaag
    19321 cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc agaaaacgag
    19381 ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat cggtttattt
    19441 gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg gggactattg
    19501 gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat cttaggtgtt
    19561 ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct gagtggatta
    19621 ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc gccactgttg
    19681 ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg gatgggacga
    19741 cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac acataaattg
    19801 agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg cctcctagca
    19861 ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg tcctcggttg
    19921 atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca
    19981 gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac gacttatgat
    20041 aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa
    20101 aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac tattgttgtc
    20161 cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc aattgatatc
    20221 aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt atcgttcaat
    20281 gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg attagatgag
    20341 gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct agaagacagt
    20401 ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt gcatgagatg
    20461 gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa agactaccag
    20521 ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac tgctaaagac
    20581 acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc ctgtactcaa
    20641 ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt ttcatcgctt
    20701 aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac agggtgaaag
    20761 agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg gaaagattac
    20821 atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa aaagtgatgg
    20881 ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat gaagttttca
    20941 aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt atcgatacag
    21001 gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt cgggaagatt
    21061 tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt acagaagaaa
    21121 agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg acacaggcaa
    21181 gcggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg gcgcgtattc
    21241 gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa ggataaagga gcgcaggaag
    21301 tagcatgaga gaaaaaagag cattttatat caatggttat gcagggattg tcggattggt
    21361 cgtattagct ttagttggat tgtttttctt ctatttaggt atgtggcaag cgaaagtttg
    21421 ggcacttttt ttaagtattt ttctttggtt gatcacgtta ttgttgttaa gttcggcaac
    21481 tgtagtcagt cctaatcaag caaaagtaat tttgtttttt ggacaatatt taggtacgat
    21541 tcgagaaaat ggctttttcc ttacgatacc tttggcacaa aaaatgactg tttcgttgaa
    21601 agttcggaat ttcaatagtt ctgttttaaa agtcaatgat ttggatggaa accccatcga
    21661 aatctcagca gtagtagtct ttaaagtcgt tgatacagca aaggcattat tcgatgtagc
    21721 gtattatcaa gattttgtag aaatccaaag tgagacagcc attcggcata tcgctagcca
    21781 atacccgtat gatacattca atgagaatga tttgacgctt cgaggaaaca cgacggcagt
    21841 gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca gtagcaggtg tcgaagtaat
    21901 cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc gctagtgcca tgctccaacg
    21961 tcaacaagca aaagccattt tgtctgctcg acaaaccatt gttgaaggag ctgtcacaat
    22021 cactcaaatg gccttagagc aaatcgaaga tggacaagaa atcaatttca ccgatgatcg
    22081 gaaagtccaa ttgatcaata atttacttgt ctcaatcatt acagacaaag gaacgcaacc
    22141 tgttatcaat acaggcgaca tgaaagaccg taagtgacat actttttgta agataggaag
    22201 aaggagttta ggacggatta ttgtcttgaa ctcttttttt agttgtttta aagaaaatga
    22261 caaaattgaa agaagaatga tagttgattc gaaggcagaa ttgttcgctt ttttctataa
    22321 tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg
    22381 ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga ttacgtagtt atgggtggag
    22441 aagaatacta tgtacaaatt acgacagatg gtacaaaaac agtttcaaaa tcagactctg
    22501 gacaagaaac agtcaactat agatatatcc tgcctggcta tacgaaagag ggtaaggaaa
    22561 aagaactgga cttcaaggga caattgactc gaccgcttcg taaaggagca tatttaaaaa
    22621 tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc
    22681 cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga
    22741 agtacagaat gtaacaaaag tatacggaaa aaacaatgag aaaaagacgc aagcattaag
    22801 cggcatcagc ttcgatgtag aaaaaggcga atttattggg atcatgggcg cttctggttc
    22861 tggtaagtcg acacttttga atattttatc cacattagat aaaccaacag atgggcatat
    22921 ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat caactagcag attttcgtgc
    22981 gaatgagatt ggctttattt tccaagattt caatttatta gaaagcttga cagcacaaga
    23041 aaatatcgct gttcctcttt ctttacaagg cgtcagacca aaagaaatca agcaacgtgt
    23101 ccaaaaaatt gcagaacgtt tatccatctc tcatattttg gaaagctatc cttctgagat
    23161 ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat
    23221 tctattaggg gatgagccta caggtgcgct ggattccaaa agtgcccggg atcttttaga
    23281 tacgatggac gaattgaata caaaagatca tgtatcgatt cttcttgtga cgcatgatcc
    23341 gttttctgca agttattgcc aacgtatttt attcatcaag gatggcggta tccatcaaga
    23401 agtgaaacgt ggagatcaaa gccgtgattc cttctatcga gaaatactat ctattcttgg
    23461 aaacttggaa cagtaggggg agattgacta tgctttggaa attatcactt actgggatca
    23521 agagccgttt aagagactac ttggtgttat tctcaggatt agttatggca tctgctattt
    23581 tttacatgtt tgaatcaatg gcaagcaacg aagctttttt gaaaagtaat acgatcgttg
    23641 cggctacagt tatcattttc cgattcggtt ctgttttact agggatcatc acattcgttt
    23701 atattttata tgcaaattcc tttttgatga cgatgcggca aaaagactat gcaatgttta
    23761 tgatgctggg agcaaaaggc agaaaaatcg cacagatgat tttttctgaa acattcttag
    23821 tagggatcgc agctacttta gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc
    23881 accgattatt agtggatcag ttagatctca atatttcgca ttttacacct tttagtattc
    23941 acgggttgtt gatcactgtt ttgttttttg ctgttttgtt tttattagct gctgttgtca
    24001 atgctttttc gattgtcaaa aaaccgattt tagcattgat tcgtgcggat cagacaccaa
    24061 caagaatcaa gcagcataaa ttcttgtttt tccttgaagt agtactggga attgcttttc
    24121 taggctgcgg ttactatatg atgaaacacg tggggaccta tcaagtcttt ggtatagggg
    24181 tagccttagt tacgatcgta ttaggaactt atttcatttt ccattctgtg atcattttct
    24241 ttttatcatt actaaagaaa tcagaacaga tttcattgaa aaaattgaac aatttcacat
    24301 tgtcacagtt aaactttcgt attcgtgaat atacacaaat gctctcaatg gtcgctatgc
    24361 tgtttgcctt ggcactgggc gctttgacgg taggattagg ttttaaaaat gaaattgcag
    24421 acttgacaga aaacaattct ggatatgatc tagtgatgaa taatcctcgg gcagaagatc
    24481 agcaaaaaat caaagaactt tcaccaacac tttctgcttc ttatactcaa aaagaagatg
    24541 cgggaacgat ttactataat gcaagtgaat ttgcggctca accattgatc atgataaaac
    24601 atgaagatgg aacaccgccg aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg
    24661 aactgactgt acaagaggaa cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa
    24721 tcaaggtagt cagtcaatca gaatttgatc aactgaactt gccacaatcc actttacagc
    24781 tagttcaggt aaaagacttc caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa
    24841 ataaaatgaa caatccatct ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt
    24901 atgaagtgta taatggtcta ttctctggtt ttgaatttat gggattcttc ctaggtttag
    24961 ctttcttgac catgcttgct agctgtttga tgttcaagat cttgtcagga agcaagagtg
    25021 acattgctcg ttatgaaatg cttgaaaaga taggaacacg acgcgggttg cttaaacaat
    25081 cgatccgtag agaagtaggc gtgttattct tagcaccagg aatcttaggt gtagtccatg
    25141 tcttattcgg attacaaatg tttggtctat tcatgcaaaa tccatacaaa gatatttggg
    25201 ttccgtttac gatattcttc gtattgtatt tcatctacta tgtactgacg acatggttat
    25261 atactggaat cgtattgaaa aagagattgt gaaaaagctg tcctgcatca agtaataaga
    25321 acagccaagc aaaaatagct cctcatattt ttcgtttggt tgggcttatt accgcagatg
    25381 caagcttttg aacaccgttt gttcggaaaa agaggttgtg acaatatttt tgtcacaacc
    25441 tttttttcac ggtcttttat tagaatatag taaatttttt ctcgttactc acggaataag
    25501 ctgttaagaa aacggtttcc atgtatactg gaatcagtgc aaatgaaaga aaagagggtg
    25561 caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa ctatcacttg attacgatca
    25621 cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc
    25681 aaacaataat cgataatgaa aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat
    25741 atctagaaaa agacccttat atcggtgctt caatcggacg tatggctgga cggattacga
    25801 atggcacatt caaattagat gatgaaacgt tcgaagtaaa aacagacccg gacactggtc
    25861 attgtttaca tggtggtgca cctagctttg aagccaagca gtggcattat acagtcatca
    25921 atggtgaaaa cgaagcatcg gtcattttct atcagactag cccagatatg gaaaacggat
    25981 ttcctggaac tttagatgta gaagtacggt atacgctgac gaacgatgat acatggcgag
    26041 tgacggtcca agggatcagt gaccgcaaaa cagtattcaa tcctacaaat catgtctatt
    26101 tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc
    26161 gattatttgc tacgctcaat aaagatacaa cgcctacagg agaagtagtt tcagtaagtg
    26221 gcacagcatt tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc agtgattttc
    26281 gccaatcaat gatagtgggc ggcatcgatc atccattttt gttgaaaaaa caatctggtt
    26341 tactaaatgc tgcggaattg acaagcccag atgaaaaagt atcgatccaa gtaaaaacag
    26401 atgcttcagg aatcgtgatt tttacagcaa attttggaga agatggaccg gaaatgcgcg
    26461 ggaataaatt agtaaatcat ggcggtatca catttgagac tcaagaactg ccaggagcgg
    26521 agcgacttga aagttttgga gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa
    26581 cggaatacaa aataaatata aagaaataga ataaattaaa aagggagtgt gacagtgtca
    26641 catccccttt ttaatttatt ctatttcttt aaaggcagat caagccagaa tgatttgtct
    26701 aaacttaaca aggttagtac aggaacagtt gcagctttaa ctgtacgagt agttgtttct
    26761 ggtatctgtc ttgccgtaaa attgttccac aaatcccaga ttgaagcgac acggaagatg
    26821 gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta atcctgtgat tggcacgcca
    26881 tctgttgtcc atttgattgg cacatcgaat cgcttagaca tattgaggat ttcagcgttt
    26941 gcttcttcag ttgttgctgc ttcttttttc ggttcatcat attttgtcag gtcgtacgct
    27001 tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg tagcataacg tccttccaaa
    27061 tatttggttg cttctttgta gtcttttgtt tccgacttcc aagttggttt gtagaaatca
    27121 ggattgccag tcagtccatt tttgatcaag tcggcatagt cttccaaact ttccttgtaa
    27181 gaaggatatt tacggaattg tgcgcggatt tggtagcttt gtccggaact atcttgttca
    27241 agtgtattga aggtcacgct gcttccttta tatgatcctt tgataccaaa aaggttatga
    27301 tttggttcgc ttgataataa gctgtttcca gatccacttt caaggatagc ttgagccatc
    27361 atgactgatg cgtagattcc ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt
    27421 ttcaagaact gatcagttgt ttggttttga ctgaaatgga acgagccatc taatccatct
    27481 aaaatagatg agccgcctgt cagcgaacct gaagcagcag gtaaacctgc taagtttact
    27541 cggacagcaa aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt atctcctggt
    27601 tgaggcgcat ggataaattg tccgttccca atatagatac ctacatggtg agtaacaccg
    27661 gaagaaggtc cccagaagac gaggtcgcca ggttgggcct gacttactgg aatctgcgta
    27721 cctgcatatt gctgttcgcc tgtccagcca ccgatataat gaccggttgc tttcatatat
    27781 acgtaataag tcaaaccaga acagtcaaaa gtatttggac cttttgctcc ccatacatat
    27841 ggttttccaa gatgcttgaa tgcttctcga acgattgctt gttggacatt cgttgtattt
    27901 gtaggcaata cagcatcaat tggtgatttt acaacaaaat cagaagaagc agaagatgga
    27961 ttctctatac cgctttgatt tgctgtattt ggacgagatg aagcattgtt cgttccgcca
    28021 ttcgatgaac cattgtttgg atggtttggt tctctctgag tatttccatt gttattcgat
    28081 ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg aattattgtt attattgttt
    28141 gaattatttg aaccattatt attgttgtta ttacccacat ctggtttgtt tggcgtttcc
    28201 ggatctggtg tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc
    28261 ggtccagctt taaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt
    28321 gcatcataat ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca
    28381 gctggcgtaa tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt
    28441 tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc
    28501 ttaccaatca aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct
    28561 gatggatcag ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt
    28621 ccagttgggt cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc
    28681 gtatctgttg tgactgtttg atcagctatt tgattatcta cggcaaatac ggtattggtc
    28741 gacaaaacag acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt
    28801 ttcataataa actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag
    28861 acattttttg gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg
    28921 tattctaaat aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt
    28981 tcattttatt gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta
    29041 tgcatatgta aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat
    29101 cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta
    29161 actattcata agacattagg tcttcatcaa acccgctaac gagagtagtg atcattgcta
    29221 cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag
    29281 gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta
    29341 cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg
    29401 caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac
    29461 cggagatcga acgggttttt gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag
    29521 ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga
    29581 tattactgat catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc
    29641 aaccaccagc gatgattgcg ccactgatcg gtccgttaat atccatcatc caagtaattg
    29701 cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag
    29761 gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat
    29821 attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga
    29881 cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc
    29941 cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg
    30001 caatatatcc attcacttta aacgttttgg cagcagaaaa agcaagaaag attggcagaa
    30061 aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa
    30121 gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca
    30181 gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta
    30241 gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca
    30301 atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag
    30361 ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa
    30421 gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca
    30481 gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa
    30541 gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa
    30601 tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt
    30661 atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc
    30721 cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa
    30781 acttggaaga ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat
    30841 gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat
    30901 atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta
    30961 gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg
    31021 cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc
    31081 tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac
    31141 ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc
    31201 ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc
    31261 tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc
    31321 atttggtata atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg
    31381 tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt
    31441 cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt
    31501 gttttttagg acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact
    31561 attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc
    31621 agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc
    31681 gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac
    31741 tgcttggttg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc
    31801 gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac
    31861 tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaattat
    31921 tttttaaaac gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa
    31981 agctatttca agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct
    32041 tcccattctt tttctgggaa ttggatccga tctaacaata acttggcaga tgtttgtccc
    32101 aattcgaaga ttggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct
    32161 atattatcat agccaataat gctgtagtct tcagggatgc tttttccagc ttcctctaat
    32221 ccgcgatata atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa
    32281 tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg agaactgtgt aggtaagagg
    32341 atcagttggt cgtgagggat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga
    32401 gcgtggacat tttccggtgg attttcagga tagacaaggg cgatcgtttg atgtcctaga
    32461 ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca
    32521 ctaaatcctt cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa
    32581 ttttcgttga ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct
    32641 cgacgtgtga gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat
    32701 aatatcgtga cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg
    32761 aagaatggat tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc
    32821 atttgtctag caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc
    32881 tgaacagttt tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg
    32941 attgaaacgc cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc
    33001 atactcctat ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa
    33061 tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc
    33121 gtactcgtgc agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac
    33181 gatcactgca agcatcttca gcaagcacga tatggtaccc gtgttgaaag gcatctaatg
    33241 cagtagcata tacaccgttt gttgtcgcta caccgcttaa aatgatagtt tcgattccac
    33301 gccggcgaag ttgaagatca agagaagtac caaaaaaagc acttggattg tattttgtga
    33361 tagtcaacat attatctgta tctttcaaag aatccttcaa aagtaaatca gtgaattctg
    33421 gaggtacaga agcagtcatt ccacccatat cagtttgttg actcaaataa gggaaagtgg
    33481 agatgtctac gttcactaaa actttcaagg cagcggtacg tttcaatgtt cttactaaca
    33541 aatcattgtt tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg ttatttacga
    33601 tctctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt
    33661 cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat
    33721 cataaatcgt ttatttgtca actaaattaa aggattcctt tctttagtct agagaaattc
    33781 agtggtaaaa taaataagtg tgagaaaacg cattcgatgc atagatatag cgagaaatat
    33841 cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac
    33901 aaaatgggag aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc
    33961 gaattagtga ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta
    34021 ggtgggatgg atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa
    34081 ttttcagata aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc
    34141 tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct
    34201 cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat
    34261 aactttccgt cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta
    34321 gctaaaaatc ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg
    34381 ggcaagcaag tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg
    34441 atcattacac ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat
    34501 gctaaagtaa ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg
    34561 tagggggatt ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa
    34621 tctaaaggac gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta
    34681 ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca
    34741 ggactttctg atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa
    34801 gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact
    34861 ggcaaaaacg aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta
    34921 caattaactg atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg
    34981 gaagatgaag ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa
    35041 aaagagtata ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga
    35101 ggatacgcga acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt
    35161 tttaaaacag atatttattc tgtcttatat cttgatttta caaacgtaaa gaatttgaat
    35221 acgtacagca aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat
    35281 ttacaagatc gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta
    35341 gaaccggcac agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg
    35401 ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa
    35461 gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa
    35521 gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga
    35581 aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa
    35641 aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt
    35701 tttcctgttt tctttttctt tattgccgca ttgatcacct ttacgacaat gacacgtatg
    35761 gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa
    35821 atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag ggataggtat tgttttagga
    35881 acgatattag gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat
    35941 gatattggga gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca
    36001 ttcttcattg ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg
    36061 cctgctgcat tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac
    36121 atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc
    36181 cgatataagt caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg
    36241 gttgcaggcg tgggattgaa agattctcta agttctgttt cagccaaaca atttggaccg
    36301 atcattgact atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa
    36361 gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa
    36421 accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt
    36481 aaaacagctg atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc
    36541 ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag aactatttga cttatctaca
    36601 ggagatcata tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa
    36661 atcgtgaaaa actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt
    36721 actgatcaat cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa
    36781 gaagaagaaa tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc
    36841 atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta
    36901 atctttgtcg tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc
    36961 aatatttccg agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa
    37021 gaagtcacga tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga
    37081 ggatttggtg taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat
    37141 gtcatctttc cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc
    37201 gtatttacgg ttattgtgat gattgttacc catttcaaat taaagcatat cgatatgatt
    37261 gatgcgttga aatcgaatga ataaaaacag aggttgtgaa atcagcggtt tgacctgagt
    37321 attagagaaa caaacggcaa aatagttttt catattttgc cgttttttga gctaataccg
    37381 aaggacagct gattgaacac catgaatcag aggttgtaaa aaagccgtcc tgcatcgagc
    37441 aaaaagaata ctcaaataaa attagctcct catgttttac atttgagaag actgattgcc
    37501 gtagataaaa gcttttgaac accatttata gaagaaacaa gaatattttg ctattttaaa
    37561 caagaggata gacaaataca tgtctgatct cttgtttttt attgggcaac agacctctta
    37621 gaatgtataa agataaattt tacaaacgct ttcggttcag ttataataat aaatgataac
    37681 ttgtatatat aagttgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca
    37741 tttaaagaca aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat
    37801 ggaattggag atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc
    37861 gacatgattt ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc
    37921 tctgattaca cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata
    37981 gccgaagcaa agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg
    38041 acagaacatg aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc
    38101 attttaagag aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca
    38161 tttggagata cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac
    38221 tggcggaacc aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa
    38281 ggggtaaaag gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa
    38341 aataataaag aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat
    38401 ctaaaagaat tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa
    38461 atgagctcta cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta
    38521 tcaatggtct ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct
    38581 actcctccat ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg
    38641 agtgagggta acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc
    38701 aatcgatttg tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg
    38761 atccatttga accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac
    38821 ccagactatt catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta
    38881 ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc
    38941 gataactctc gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg
    39001 aaaccttggt taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct
    39061 tctgaagatt ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca
    39121 atcgttgcac aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt
    39181 cttcgccaat ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca
    39241 aggatcgcca tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat
    39301 actgttcttc atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat
    39361 taaaacaaaa acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg
    39421 ttttatttaa aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca
    39481 taaaatttat tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact
    39541 aacgtataga taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca
    39601 agtaaaacga ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga
    39661 atcaccaagt caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc
    39721 ataaaagtag agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca
    39781 atatatggaa tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat
    39841 ccgatcagta agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc
    39901 ccctttagaa atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta
    39961 cgaaatgctg gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa
    40021 aggaaaaaag cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa
    40081 aaaatgttgc tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata
    40141 tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt
    40201 ttctccaaaa attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg
    40261 atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat
    40321 aaaaataaaa taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc
    40381 aaacgtttga tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc
    40441 aagatagtag cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa
    40501 taaatgatca gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct
    40561 aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta
    40621 tctataactt cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca
    40681 aaaatatcgt caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac
    40741 agttctttta accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg
    40801 cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa
    40861 acgattgata ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc
    40921 aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat
    40981 gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc
    41041 agggactgat tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac
    41101 agatatcttt gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac
    41161 attacaagaa ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg
    41221 aagtgaagaa ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct
    41281 agacccacat gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga
    41341 tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta
    41401 taccgagaaa cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca
    41461 aagatctttt gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa
    41521 tcaagtatcg attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga
    41581 actgaaacac tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa
    41641 agatagtatt gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc
    41701 tttggaagga ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga
    41761 agcaaacctc gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt
    41821 gacccccaaa aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga
    41881 tcgctctctt tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg
    41941 gacttttgat cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga
    42001 atataaagaa tattatacga agggctatca aacagatatt gcttcaatca agatcagtga
    42061 gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt
    42121 agggaaagaa atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc
    42181 agaagataag gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc
    42241 ggctgactcc gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa
    42301 agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca
    42361 ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt
    42421 tagtcggtcc aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga
    42481 ggctgggatg gtctcgccgt ttgccaacgt ggcttctctg gtttttttat taatcgaccg
    42541 cacattttgt gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt
    42601 ctcgatttct ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag
    42661 tttatgtggc gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt
    42721 gatctcatca ttaaactcta ttttgaagat atctttttgg atttgggaca tttgaaaatc
    42781 ttcgaaaatt ttgctcagga tatgctcgac attttctttt aaatcataaa gatcttcttt
    42841 gacgatataa tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca tttccgtatg
    42901 ggaagtcaca tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc
    42961 tacaccatcc atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga
    43021 agttctgatc ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata
    43081 actgtctaag ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg
    43141 ttttggatcg tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg
    43201 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
    43261 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
    43321 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
    43381 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
    43441 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
    43501 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
    43561 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
    43621 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
    43681 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
    43741 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
    43801 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
    43861 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
    43921 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
    43981 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
    44041 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
    44101 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
    44161 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
    44221 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
    44281 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
    44341 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
    44401 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
    44461 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
    44521 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
    44581 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
    44641 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
    44701 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
    44761 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
    44821 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
    44881 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
    44941 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
    45001 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
    45061 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
    45121 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
    45181 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
    45241 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
    45301 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
    45361 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
    45421 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
    45481 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
    45541 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
    45601 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
    45661 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
    45721 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
    45781 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
    45841 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
    45901 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
    45961 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
    46021 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
    46081 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
    46141 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
    46201 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
    46261 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
    46321 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
    46381 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
    46441 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
    46501 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
    46561 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
    46621 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
    46681 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
    46741 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
    46801 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
    46861 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
    46921 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
    46981 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
    47041 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
    47101 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
    47161 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
    47221 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
    47281 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
    47341 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
    47401 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
    47461 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
    47521 aaaggaaaaa ttttcagaaa actaaaatca tatcgcttcc cattttcatt tttagaaagg
    47581 tctgttagta taaagaaaaa atagacaacg aaactttaaa taaccaactt gaaataaaca
    47641 aattcaaaga agaaagatgg gaaggaaatc aatcagttga atgaaaaaga aattctccga
    47701 ttacaaaaag aattgaaacg ctgggagaac aagtataaaa agcttcaaca agtctttcgg
    47761 ctacaagaag aaatcattgc tacctatgaa gcggcttatg gcgaagcacc cggacttatt
    47821 gaggaagaag tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa aatgatggag
    47881 aaacgttcct ataatcgctt caatcaagaa caaaaagcag ctatcacata tgatatgaag
    47941 aaacatcttc gaatcatagc aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa
    48001 gctgtttatc tgatgacgca aaaacaagta gacgaagaac gcatactaat gatcactttt
    48061 acaaggaacg ctgcaaatga actaaaaaaa cgagtagata atttcagcca gagaaaaacc
    48121 gctgtccata tcggaacatt ccatagtatc ttttttagga tatacaatga aatttgtcga
    48181 aaatttcctg aagtagcaat gcaaggaata caaggagact tttcaaagga tactgcacaa
    48241 aaagtgaatg ctgttttgca acagctgata cgtaaatata atctttatat atttgatcaa
    48301 tatggagaaa aaacgattgc ttctcggtta gactattggc agaatatgaa cctttcagta
    48361 gaagaaatgg ttcagttagt taaagaaaaa tatgattcca ttgataagaa tgcacgacag
    48421 ccgatatcag aacgactgta tggattgctc acggaactcc aagaacaaaa aagaagccag
    48481 caattgctag agttcaatga tgtactccaa aatctgaaaa gcgctttgga gaatgaggag
    48541 atccagcatt atatcggcca aaaatatgat tatctattca ttgatgaatt tcaagatact
    48601 aatccacttc aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat caagttgatt
    48661 gttgttggag atgatgatca gagtatctat ggattcagag gatcagagcc agcatacatc
    48721 aagaactttg aaaaagaata tcctaccaaa actcttttct tattaactaa ttatcgttct
    48781 cgggcagcta ttgtccaagg agcaaataga ttgatcagct ataataaaaa tgatcggata
    48841 ccaaaggcga tgattccagc acaaaaagaa gcaggtgtta tggaagccta tttgttttct
    48901 gatactaaag cagaaagtca gtggctaatc aaacaaattc agacatttat tcaaaaaaat
    48961 gggaaatata aggaatcaat catattatac cgatcagctt cacaaacgca gcagcttgtc
    49021 caatctctgc tgaaaacgaa tattcctttt gtactggaag cagatagtcc atatgaaggt
    49081 atctttggta tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca
    49141 ctgaatctgc aaaagaaaaa tcaggcctac cagcaattgc tccggcagct gatggcagac
    49201 tgctacctga aaaaaagtga atgcgatcaa tacttttctc ctaaatatca aaatatagcc
    49261 gttgcagatt atattttgtc gataagcccc aatttaaaat cgaaatctgc agaaatccga
    49321 gagtttgagg aagcgttgcg taaaacagac cagcatcaag aaatctattc gcttgttcaa
    49381 agctatcttc atctgccacg tatagctaag gaaatcgacc agagtgatca agaatggata
    49441 cgagaagaag taaagcagca tgctactttc agaagactcc aagccttatc gcaagaaacc
    49501 caagcgacag ctaaagaatt aaaaacccgc ttgattgctt acagacaagg aaaacttgat
    49561 gcactatgta tccagagcat tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg
    49621 attggttgta acgaaggaag tttgccttat aacggagcga cagaaaagaa agtaatcgac
    49681 tttaatgaaa taaaagcaga accagtaacg acgatagaag aagaacgaag actattttat
    49741 gttgcgatga ctagagcaag aaatcgtctg tatctatgtg tacctcaaat gaaaaattca
    49801 agaaaattga aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag
    49861 tgatagaaga gagggtgtga cagaagtggt cagtttcaag aaagaagaag gaatgtacga
    49921 aaattgcttc tcaaattttt gtgaaatttc agcttatttt cgaagaagct atttctggaa
    49981 cacagtttat tcggaaaagg
//
LOCUS       DAZUMT010000007        45928 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000007 DAZUMT010000000
VERSION     DAZUMT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.007"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(241..609)
                     /locus_tag="ACG8FM_000374"
     CDS             complement(241..609)
                     /locus_tag="ACG8FM_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS5093067.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            759..1241
                     /locus_tag="ACG8FM_000375"
     CDS             759..1241
                     /locus_tag="ACG8FM_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS5093068.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1278..2288
                     /locus_tag="ACG8FM_000376"
     CDS             1278..2288
                     /locus_tag="ACG8FM_000376"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS5093069.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2403..3116)
                     /locus_tag="ACG8FM_000377"
     CDS             complement(2403..3116)
                     /locus_tag="ACG8FM_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093070.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3289..3896)
                     /locus_tag="ACG8FM_000378"
                     /pseudo
     CDS             complement(3289..3896)
                     /locus_tag="ACG8FM_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4380..4607
                     /locus_tag="ACG8FM_000379"
     CDS             4380..4607
                     /locus_tag="ACG8FM_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS5093071.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4623..5789
                     /gene="hemW"
                     /locus_tag="ACG8FM_000380"
     CDS             4623..5789
                     /gene="hemW"
                     /locus_tag="ACG8FM_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS5093072.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6023..7066
                     /gene="hrcA"
                     /locus_tag="ACG8FM_000381"
     CDS             6023..7066
                     /gene="hrcA"
                     /locus_tag="ACG8FM_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS5093073.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7160..7723
                     /gene="grpE"
                     /locus_tag="ACG8FM_000382"
     CDS             7160..7723
                     /gene="grpE"
                     /locus_tag="ACG8FM_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS5093074.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7774..9603
                     /gene="dnaK"
                     /locus_tag="ACG8FM_000383"
     CDS             7774..9603
                     /gene="dnaK"
                     /locus_tag="ACG8FM_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS5093075.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9754..10920
                     /gene="dnaJ"
                     /locus_tag="ACG8FM_000384"
     CDS             9754..10920
                     /gene="dnaJ"
                     /locus_tag="ACG8FM_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS5093076.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11002..11631)
                     /locus_tag="ACG8FM_000385"
     CDS             complement(11002..11631)
                     /locus_tag="ACG8FM_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093077.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11643..11816
                     /locus_tag="ACG8FM_000386"
     CDS             11643..11816
                     /locus_tag="ACG8FM_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093078.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11861..12598)
                     /locus_tag="ACG8FM_000387"
     CDS             complement(11861..12598)
                     /locus_tag="ACG8FM_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5093079.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12600..14102)
                     /locus_tag="ACG8FM_000388"
     CDS             complement(12600..14102)
                     /locus_tag="ACG8FM_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS5093080.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14361..16355
                     /gene="uvrB"
                     /locus_tag="ACG8FM_000389"
     CDS             14361..16355
                     /gene="uvrB"
                     /locus_tag="ACG8FM_000389"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS5093081.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            16365..19181
                     /gene="uvrA"
                     /locus_tag="ACG8FM_000390"
     CDS             16365..19181
                     /gene="uvrA"
                     /locus_tag="ACG8FM_000390"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS5093082.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19444..20709
                     /locus_tag="ACG8FM_000391"
     CDS             19444..20709
                     /locus_tag="ACG8FM_000391"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS5093083.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20713..21444
                     /locus_tag="ACG8FM_000392"
     CDS             20713..21444
                     /locus_tag="ACG8FM_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS5093084.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21579..22463
                     /gene="rapZ"
                     /locus_tag="ACG8FM_000393"
     CDS             21579..22463
                     /gene="rapZ"
                     /locus_tag="ACG8FM_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS5093085.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22460..23458
                     /gene="yvcK"
                     /locus_tag="ACG8FM_000394"
     CDS             22460..23458
                     /gene="yvcK"
                     /locus_tag="ACG8FM_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS5093086.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23492..24427
                     /gene="whiA"
                     /locus_tag="ACG8FM_000395"
     CDS             23492..24427
                     /gene="whiA"
                     /locus_tag="ACG8FM_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS5093087.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24995..25651
                     /locus_tag="ACG8FM_000396"
     CDS             24995..25651
                     /locus_tag="ACG8FM_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093088.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25830..26395
                     /locus_tag="ACG8FM_000397"
                     /pseudo
     CDS             25830..26395
                     /locus_tag="ACG8FM_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27094..27615)
                     /locus_tag="ACG8FM_000398"
     CDS             complement(27094..27615)
                     /locus_tag="ACG8FM_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS5093089.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            27813..31493
                     /gene="nifJ"
                     /locus_tag="ACG8FM_000399"
     CDS             27813..31493
                     /gene="nifJ"
                     /locus_tag="ACG8FM_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS5093090.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            31682..32569
                     /gene="cdaA"
                     /locus_tag="ACG8FM_000400"
     CDS             31682..32569
                     /gene="cdaA"
                     /locus_tag="ACG8FM_000400"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS5093091.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            32566..33735
                     /locus_tag="ACG8FM_000401"
     CDS             32566..33735
                     /locus_tag="ACG8FM_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS5093092.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            33759..35114
                     /gene="glmM"
                     /locus_tag="ACG8FM_000402"
     CDS             33759..35114
                     /gene="glmM"
                     /locus_tag="ACG8FM_000402"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS5093093.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(35186..36991)
                     /gene="glmS"
                     /locus_tag="ACG8FM_000403"
     CDS             complement(35186..36991)
                     /gene="glmS"
                     /locus_tag="ACG8FM_000403"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS5093094.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            37401..37691
                     /locus_tag="ACG8FM_000404"
     CDS             37401..37691
                     /locus_tag="ACG8FM_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093095.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            37704..38729
                     /gene="sppA"
                     /locus_tag="ACG8FM_000405"
     CDS             37704..38729
                     /gene="sppA"
                     /locus_tag="ACG8FM_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5093096.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            38732..39391
                     /locus_tag="ACG8FM_000406"
     CDS             38732..39391
                     /locus_tag="ACG8FM_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5093097.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            39548..40480
                     /locus_tag="ACG8FM_000407"
     CDS             39548..40480
                     /locus_tag="ACG8FM_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093098.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            40634..41923
                     /gene="tig"
                     /locus_tag="ACG8FM_000408"
     CDS             40634..41923
                     /gene="tig"
                     /locus_tag="ACG8FM_000408"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS5093099.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            42170..43420
                     /gene="clpX"
                     /locus_tag="ACG8FM_000409"
     CDS             42170..43420
                     /gene="clpX"
                     /locus_tag="ACG8FM_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS5093100.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            43503..44090
                     /gene="yihA"
                     /locus_tag="ACG8FM_000410"
     CDS             43503..44090
                     /gene="yihA"
                     /locus_tag="ACG8FM_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS5093101.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(44164..44319)
                     /locus_tag="ACG8FM_000411"
     CDS             complement(44164..44319)
                     /locus_tag="ACG8FM_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS5093102.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            44455..45783
                     /locus_tag="ACG8FM_000412"
     CDS             44455..45783
                     /locus_tag="ACG8FM_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093103.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        15141 a         7893 c         9852 g        13042 t
ORIGIN      
        1 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtaga
       61 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
      121 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
      181 aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
      241 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
      301 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
      361 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
      421 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
      481 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
      541 aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga tcggctgaaa
      601 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
      661 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
      721 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
      781 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
      841 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
      901 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
      961 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     1021 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     1081 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     1141 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     1201 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     1261 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     1321 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     1381 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     1441 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     1501 aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag attctcgatt
     1561 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     1621 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact aaccttattg
     1681 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     1741 gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg tggccccatg
     1801 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     1861 gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg atcaggcatt
     1921 taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     1981 cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga agatcacttg
     2041 ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     2101 cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg tcaaaggaat
     2161 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     2221 ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     2281 ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat ctaaatagaa
     2341 aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac atattagaaa
     2401 gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg atttcctgta
     2461 ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct aatgaacgct
     2521 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     2581 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     2641 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     2701 gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa agtattacct
     2761 ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     2821 tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga aatatttgtc
     2881 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     2941 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     3001 tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     3061 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     3121 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     3181 accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga aaaagtgtga
     3241 caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt aattgaagag
     3301 aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc aagtttgtat
     3361 agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg aagaatcaag
     3421 tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc tttcacagct
     3481 tgttgttcta taaaatcttc tttattctga taattttccg attctaacca aggaaattgc
     3541 gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata atcaactgcc
     3601 ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat ttgatataaa
     3661 tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc aactttttca
     3721 ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga agtgacctct
     3781 gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt gctagtaata
     3841 taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt tttcattttc
     3901 aagctctcct ttactatcta taacaaggac aacagtttta acctacagac attcattaaa
     3961 attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat acagatagtc
     4021 aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt taccagcatt
     4081 ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg agtacaatat
     4141 taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca tatcgggcac
     4201 tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat gaaagaaaaa
     4261 atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa tacgtttagt
     4321 atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag gaggaatctg
     4381 tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc agctttgcga
     4441 ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat aagcaagcat
     4501 ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag ttggtgcttg
     4561 aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga gaaagaattt
     4621 caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt catatcccat
     4681 tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa gggcagccag
     4741 tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa agtcatccga
     4801 cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta tctgctaaac
     4861 aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat ggtaatgagt
     4921 ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta atgaaaaact
     4981 atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta ttgaaaaaaa
     5041 ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta gaagcagaaa
     5101 attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca ctagaaggat
     5161 atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct ttgtactcat
     5221 tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga ctcgaactgc
     5281 ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg gaaaagcatg
     5341 gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct caacataacc
     5401 tcatgtactg gaacaatgcc cactattttg ggtttggagc tggtgctagc ggttatcttg
     5461 gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca ttacgagcaa
     5521 atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata gaagaagaaa
     5581 tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat gatcgctatc
     5641 aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa gaagggttac
     5701 ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg gggaacaatg
     5761 tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt atctgaattt
     5821 aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat aactggattt
     5881 acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg gagtctttct
     5941 cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta gaatatatga
     6001 gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt tacgtctgat
     6061 catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga tgtctgaggg
     6121 agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag atgaaggatt
     6181 gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct atcgttatta
     6241 cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc tgattcgtca
     6301 gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg ctgagatatt
     6361 atctgagctg acaagctata cgactttttc attgggacct gaaataaaag accgccgact
     6421 gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg tcgtaacaga
     6481 tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa gtcaggaact
     6541 agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga tgacggtgta
     6601 ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa cgacagaagg
     6661 gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg tttatgtcag
     6721 tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt tcaaatcgat
     6781 gttttctttt atgaaagact cagacggact tacacaaatg atcactccga tggatagcaa
     6841 tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata tgagtatgat
     6901 tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt tagggcctac
     6961 tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag aactagcagc
     7021 taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg aataacgaag
     7081 ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc tgactgaata
     7141 atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag aaatgacaga
     7201 tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta tttcagaagc
     7261 tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa tggaagataa
     7321 atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa atgagcgtga
     7381 gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat caattgacaa
     7441 cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt tgaaaaaagg
     7501 cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa ttgaaaaaat
     7561 ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa ctgttccagc
     7621 gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct acaaattaca
     7681 tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg gatataaaac
     7741 ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg tattgactta
     7801 ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat catcgctaac
     7861 ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga aatccaagta
     7921 ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc aatcaaacgc
     7981 catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac accacaagaa
     8041 atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt aggtgaaaaa
     8101 gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg tcaagcaaca
     8161 aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga accaactgca
     8221 gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt agtattcgac
     8281 cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt ctttgatgta
     8341 ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa aatcatcgac
     8401 tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga caaaatggct
     8461 ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg cgtatcaagc
     8521 acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca tttggaaatg
     8581 acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg tacaaaagta
     8641 ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga cgaagttatt
     8701 ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa agaaacagga
     8761 aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc tgctatccaa
     8821 ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac accattgtca
     8881 ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa tacaacgatt
     8941 ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc agtagacatc
     9001 catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg aagattccaa
     9061 ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac attcgatatt
     9121 gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa agaacaaaaa
     9181 atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat ggtaaaagat
     9241 gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct acgtaatgat
     9301 gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg caaagtggac
     9361 gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc tgttgaagca
     9421 aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt tcaaaacttg
     9481 actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc agaagcagct
     9541 caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg cgacgataaa
     9601 taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt tttcttttga
     9661 aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata acaagcgacg
     9721 tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga ttattatgaa
     9781 gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta tcgtaagctt
     9841 tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa attcaaagaa
     9901 gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata tgaccaatac
     9961 gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg atttggtgga
    10021 ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga ctctttcttt
    10081 ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga tttgcaatat
    10141 acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat caagtataat
    10201 cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac acaaccaact
    10261 acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac accacttgga
    10321 cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga aatcaaagaa
    10381 ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt taaagtgaat
    10441 gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg agaagctgga
    10501 gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga aagtgatatc
    10561 tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt acaagcagct
    10621 ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa gatccctgct
    10681 ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa gttacgtggt
    10741 ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa tctaaatgaa
    10801 caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt tgaagaacag
    10861 caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa gcggaagtag
    10921 tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca atcatatgtt
    10981 cgcctggttt ttttgtagtt gtcatgtttg taccaacaaa aaagccagct ttagaatatg
    11041 ataatcgtca tagtttctag gggtataacc agtgagagaa tggaacttgt tcaatcgata
    11101 ttgcaaggtg tttttatgaa taaataattc ttctgagcaa tttttgatgc ttccgttatt
    11161 tttactgaag catgccaaag tcttcttgaa caaatgtact tcttcttctg tcaggctttt
    11221 caaaatacgt tttacatata atgttcgttg atctgacggg attgctggta aaacgatccc
    11281 gaggtctaaa tcttcaaaaa agcggatctc gttcacagat tgatggaaag ctatccattc
    11341 gcaaacttgt ttggcatgaa ggaaagattg cggtgtctct tgctctgtat aacaaagcga
    11401 cccaattcca aagtttagct gtaactgata cattttatta aactttgtct ggattttatc
    11461 aagcaatgat agcaatgagg ctctggaaat ccgattgact aggataatga tttgttgtct
    11521 atgtacagtg agcaaagcct gtccgtaata agagaagacc ctttcgagca actccaataa
    11581 aggattgatc cgttcttgac acaattgctg attgtcagaa agttgtccca caactacata
    11641 atatggagaa gaaaaatcaa gctcaattaa gtgagggtgg tatcgactat gtacttgata
    11701 tcatcaatga accgatggta ctttcttatg cgatggaaca ggtagagcga ttgcttgttt
    11761 gtgcaggaaa acaagcgatg cggctgatac gattaggtga gaaaataaga agataaaaat
    11821 acacggtccc ttcatggcga ccgtgcattt ttggttttta ttaaatattt aatactttat
    11881 tcaagaaatc ttttgtccgt tcattttgcg gattttcaaa gatttgttga ggcgttccat
    11941 cttctaaaaa gtttccgtcg tcaatgaaca tgacgcgatt ggccacttct ttagcgaacc
    12001 ccatttcgtg cgtaacgata accatcgtca taccttgttc tgctaacttt ttcatgacgt
    12061 tcaagacatc accgaccatt tcaggatcga gtgcagaagt aggctcatca aaaagcatga
    12121 tgtcagggtt catcgctaga gcacgagcaa tagccacacg ctgtttttgt ccaccagaaa
    12181 gcatttcagg atacatatct tttttatctt ctagaccaac tgtctgcagt aatttgattg
    12241 cttttttctc tgcttcttct ctagattctt tcttcaggtc catcggtgct aaaatgatgt
    12301 tttccaaaat agaaagatga gggaataggt taaagtgttg gaataccatt ccgatatgtt
    12361 gtctcaccaa gttgatatct gtgcttttat ccatcaagtg ggcgccgtca ataataattt
    12421 caccgtcaga aggttcttct aaacgattca agcaacgcaa gaatgtactt tttcctgaac
    12481 ctgaagggcc aatgatacag acgacatctc cttcgttgat ggatacattg atgtcgttta
    12541 atactgtgtt atcgccgtat tttttgacta aatgctcaac taagattttt tctgccatct
    12601 tacttcacct tcttttctaa aacttttgct aattttgtca atgctgtgat caaaatcaag
    12661 tacatgatcg caatgatcaa gtaaacatac gtactttgtg ttgtacgtgc aacaatgatc
    12721 ttaccagttt gcagcaattc gactacaccg attgcggaaa tgatcgttgt gtcttttaat
    12781 gagataacga actgattgac gaatgatggg atcatgatgc gtatcgcttg cggcaaaatg
    12841 attttttgca ttgtccgatt ataagaaaga cctaaacttc tggaagcttc catttgtccg
    12901 acaggaacgg cgttgatccc gcctcgaaca atctctgcga tataagcact tgcgttaaga
    12961 gtcaaggtga taacaccagc aaggaagtca ggaatcgtaa atccaacgat accaggcaac
    13021 ccaaagaaaa taaagaacgc aagaaccatc attggaattc ctcggatgat atcgacataa
    13081 atactagcaa ttgtgcgaag agcgcgtatt ggtgctacac tgaataatcc aaaaatcaca
    13141 ccgattacta tagccaaagc aaaggagatc aaagttagga gaattgttct ccaaagtcct
    13201 ttcaacaatg cttgatagtt gttttggata agtccaagaa atgttgattc atcagcagtg
    13261 gaagactcac tggaagtttc tgaaccatcc gctacatatt ctttaataat cttatcgtat
    13321 tcgcctgttc tttttaattc tttcaatcct tcattgaaca tattaagaag ctcaggattt
    13381 tgtccttttt taacggcaaa tgcatatttt ccgccagatt cttttttgat cggtgtttcc
    13441 aattcttgtc cttgtttgat cgcataaccg attaccggat aatcatccat cattgcatcg
    13501 attgcgccaa cttttaaccc gtcgtacaat tgatcagcag catcaaaata tttgatagaa
    13561 tagccatatt cagattcatg gtcgcttaaa aattcagcac tttcggtacc aacttttgct
    13621 ccgactgttt ttcctttcaa atcttcataa gacttgatcg aagtatctcc ttttttgaca
    13681 gctaattgga tcccgctatc aaagtaagaa tcagaaaaat catagctttt ctttcgttca
    13741 tctgttacag tcattccggc aatcattccg tctgcttgtc ctgattccaa agcttgcaaa
    13801 gcagcagaga aaccacggta gtccattcgg atgttaaatc cttgcagttt ggcgatgttt
    13861 tccatcaatt ttacatcgat gccttcgtac tcgccctttg cgttttgaaa ttcaaatgga
    13921 gcaaaagcag agtcactggc aattacgtat acgtcttttt tgggctctat tttttgcatt
    13981 gtctcgcttg tgcttgactc tgccgttgta gtgttgtcag cttgtgcaac atgaccaaac
    14041 gcaagtgtca caagtcctgt cattaagaaa acaaatgagt gaaataaatg ctttcttttc
    14101 ataatcaacc tcctaaattt ccaatatttt attattatag gcgaaaagtg ctgaatcaga
    14161 caagactgta agctcgaaaa aatgaaaaaa atgcgagttt ctaacatttt atgttataaa
    14221 acataacatg tataataaga taaaaaagtg gcgaaagtat gttcggtgtg gtataatttt
    14281 ttagtaagga atgcctttgc tttccttttc tttttattgg tcgtaaaatg aaaaggaatg
    14341 aatgaaaagg aggacgaaga atgattgaga gagatacctc ccgacacttt gagcttgtct
    14401 cgaaatacca accggctggt gatcagcctg aagcgattaa ccaattagtg gatggcgtag
    14461 tcggcgggaa aaaagcacaa atcttattag gtgcgactgg aacagggaaa acatatacga
    14521 tttccaatct tattgagaaa gtgaataaac caacactgat tattgcccat aacaaaacat
    14581 tggctggtca gctgtacggg gaattcaagg aatttttccc taacaatgct gttgaatatt
    14641 ttgtttctta ttatgattat tatcagcctg aagcttatgt gccttcaagc gatacgtata
    14701 tcgaaaaaga ttcaagtgta aatgatgaga tcgacaaatt acgacattcg gctactagtt
    14761 cattattaga aagaaatgat gtgatcgtca ttgcatccgt ttcatgtatt tttggattag
    14821 gctcgccttt tgagtaccaa aaacaagtag tttcgattcg acagggagca gaactagatc
    14881 gcaatcagtt gattcgtgac ttagtcagta tccaattcga aagaaatgat atagattttc
    14941 agcgcggtcg ttttcgagtg cgcggtgatg tcgtggagat ttttccggct tcaagagatg
    15001 aacgtgcctt acgtgtggag ttttttggag atgaggtcga acgtatccga gaagtcaatg
    15061 cactgacagg tgaagtttta ggagaaacgg aacacgtagc aatcttcccg gcgactcact
    15121 ttgtgacgaa tgatgaacat atggagcatg ccgtagcgaa tatcaaagcg gaattagaac
    15181 agcgtttaac tgtcttgcga aatgaaaaca aacttctgga agctcaacgt ttagaacagc
    15241 gaaccaatta cgatattgaa atgatgttag aaatgggcta cacgtcggga attgaaaatt
    15301 attctcggca tatggacgga cgtaaagaag gtgaaccgcc ttatacgcta ttggatttct
    15361 ttcctgaaga ttttttgatc gtggcggacg aatcgcacgt aaccatgcct caaatcagag
    15421 gaatgtataa tggtgaccga gcgagaaaac agatgctagt agattacggt ttccgtttgc
    15481 catctgcttt agacaatcgg ccacttcggt tagaagagtt tgaaaaacat gtcaatcaga
    15541 tcatttatgt ctctgcgacg ccaggaccat atgaacatga acaaacagat accgttatcc
    15601 aacaaatcat ccgtccgaca gggctgcttg acccagtgat cgaagttcgt ccaatcatgg
    15661 gacaaatcga tgatctagta ggcgaaataa atgaacgagt cgaaaaagac cagcgggtgt
    15721 ttgtaacgac attgacgaaa aaaatggcag aagacctcac agattatttc aaagaattag
    15781 ggatcaaagt caaatatttg catagtgata tcaaaacgct ggaacgaaca gaaatcattc
    15841 gagatcttcg attaggtgaa tttgatgtgt tagttggaat caatttgctt cgtgaaggat
    15901 tagacgtacc tgaagtttcc cttgttgcta tcttggatgc ggataaagaa ggctttttac
    15961 gtagtgaacg ttccttagtc caaacgattg gacgagctgc tcggaatgaa gaaggtaaag
    16021 taatcatgta cgcagacaaa gtaacagatt ctatgcgtct agcgatggat gaaacgtcaa
    16081 gacgtcgtac gattcagcag aagtacaatg aagaacatgg gatcgttcca aaaacgatca
    16141 ttaaagaaat ccgagatttg atttctatta cgaaagaatc ggaagatgat acaaaagaag
    16201 cggtacaagt ctcgtatgaa gaaatgacga aagaagaaaa agatacgtta ttgatgaaat
    16261 tagaaaaaga aatgaaaaat gcggcgaaag cattggattt tgaaacagcc gctaatgtgc
    16321 gagatatgat tttagaatta aaagcttcaa agtaggagga caacatggct aacgataaaa
    16381 ttacgattca cggtgcacgt gcccataact tgaaaaatat agatgtaacg attccacgag
    16441 ataaattcgt ggtcgttaca ggtttgtccg gttctggaaa aagttcccta gcatttgata
    16501 cgctgtacgc tgaagggcag cggagatacg tagagagcct ttctgcttat gctcgtcagt
    16561 ttttaggaca aatggataag ccggacgtag atagcatcga cggattgagt ccggcgattt
    16621 ctattgatca gaaaacaaca agtaaaaatc ctcggtcgac agtaggtact gtgacggaaa
    16681 tcaatgacta tttacgcttg ttgtttgcac gtgtaggaca tccgatttgt cccaacgatc
    16741 atatcgagat taccagtcaa tcagtagatc aaatggcaga tcaagtaatg gctttgcctg
    16801 agcgcacaaa gatccaagtg ttggcaccga tcgtggtaaa gaaaaaagga caacataaaa
    16861 aaatattcga acgaatccaa aaagagggat acgtccgcgt tcgagtcgat ggtgagatct
    16921 atgatttaag tgaagtgcct gagctggaga aaaacaaaaa acatgatatc gcgatcgtca
    16981 tcgaccggat cattgtgaaa gaagggattc gttctcgtct atttgattct ttagaggcgg
    17041 ctctgcgatt ggcagacggc tatgctctgg ttgacgtgat cggtgaagaa gaaatgttgt
    17101 ttagcgaaca ctatgcctgt ccttattgcg gatttactgt tggcgaacta gaaccaagac
    17161 tgttctcatt caatgcgcct tttggagctt gtccagactg tgatggttta ggtgtcaaat
    17221 tagaagtcga tcaagattta gtgattccag atagaacgaa gacacttagt caaggtgcta
    17281 tcgttccatg gaatccaatc agctctcagt attatcctca aatgttagcg caggcttgtg
    17341 aaagtttcgg tatcgattta gatacaccat tcgaagaatt accggaagag catcaggata
    17401 tcatcttgaa tgggacagat gatcttttcc atttccatta tgaaaatgat tttggtggcg
    17461 ttcgcgatgt agaaacgact ttcgaagggg tattgaagaa tatcgagcgt cgttatcatg
    17521 atacgaacag tgatttcacg cgtgagcaaa tgcgtcttta tatgacagaa ttaacttgtc
    17581 agacatgtaa aggttatcga ttgaatcccc aagctctatc tgttcaaatc aatggaacga
    17641 atatcgggca aacaaatgag ttgtctattc aaaaagcagt agatttcttc tcaaacttaa
    17701 cgttatctga acaagagaaa gtaattgcca aaccgatttt aaaagaagta aatgacagac
    17761 tttcattctt ggaaaatgtg ggactagact atctaacttt gagtcgcgct tccggaacat
    17821 tatctggggg agaagcgcag cgaatcagat tggctacaca aattggatcg aatctttctg
    17881 gagttttgta tattttagat gaaccatcga tcggattgca tcaacgcgac aacgatcgac
    17941 tgattggctc attaaagaaa atgcgtgatc ttggcaacac cctcgttgta gtcgaacatg
    18001 atgaagatac gatgtgggca gcagattatc tgattgacgt cggacctggg gccggagaac
    18061 aaggcggaca aatcgtggct gcaggcactc ctgaagaagt agctaatgat gaaaattctt
    18121 tgacagggaa atatctttca gggaaaaaat caatccctgt accaaaagaa cgccgtaaag
    18181 gtaatgggaa agcaatcaag atcactggtg cttcagaaaa caatctgaaa aatattagtg
    18241 ttgaatttcc attaggagaa tttgtagcag tcacaggtgt gtctggttct ggaaagagta
    18301 cattggtcaa tagtatctta aagaaatcgt tagcgcaaaa attaaataag aattctgcta
    18361 agccaggtaa attcaagaca atttccggct acgaaagtat cgaaaagatc atcgatatcg
    18421 atcaaagccc aatcggccgg acgccgagaa gtaatccagc gacttataca agtgtatttg
    18481 atgatatccg tgggttattt gctcaaacga acgaggcaaa aatgcggggt tataagaaag
    18541 ggcgttttag tttcaacgta aaaggcggtc gttgtgaagc ttgtcgcggg gatggaatta
    18601 ttaagatcga aatgcacttt ttgcctgatg tctatgttcc ttgtgaagta tgtcatggca
    18661 aacgatataa ctctgaaaca ttagaagtgc attacaaagg aaaaagcatt gctgatattt
    18721 tggaaatgac agtagaagat gctgtagaat tcttcaagca cattccaaag attcatcgca
    18781 aactgcaaac gattgttgat gttggcttag gttatgtgac gatggggcaa ccagcaacga
    18841 cattgtccgg tggtgaggca caacggatga aacttgccag tgaattgcac aaaatctcta
    18901 atggaaagaa tttctatata ctagatgaac caacgacagg acttcatagc gatgacatcg
    18961 cccgcttgtt gcatgtatta caaagattag tagatgctgg taacacagtt ttagtgattg
    19021 aacacaatct agatgtaatc aaaacagcag attatatcat tgatttagga ccagaaggtg
    19081 gagaaggtgg aggaacgatc cttgcgactg gaacaccaga agaaatcatt aacgtaaaag
    19141 aaagttatac aggtcactat ttgaaaaaaa taatggtata aagaggatag atagctgaga
    19201 tagttaaaaa aacataaaaa aatgaatttt tgcttgtttt attaatatca gtgttctaga
    19261 atcattacat ggtagttctt tctacactga ttggtataga caaaaacttc ctataagttc
    19321 aatccgttca aaaaagaatt gagcaatcgg aggagtttcc tttgattgct tttttcttta
    19381 tggtatgata ttccttgtgt agttggaaaa gaaaaacaag agattatgag taagtgagag
    19441 ataatgatga acagtattga aaatgaagaa tttatcccta tattattagg aagcgacatg
    19501 aacgtttatg gtatggcacg ttcttttaat gaagcatacg gcaaaatctg ccaagcatat
    19561 gcttcggatc agttagcccc tacccgctac agtaaaattg tcaatgtaga agttattcca
    19621 ggatttgata aagaccctgt gtttatcgag acgatgcttc gtttggcaaa agaaagatat
    19681 tctgataaga gtaaaaaata tcttttgatt gcttgtggtg atggatatgc agaattgatc
    19741 tcccaacata aacaagagct gtcagaatat tttatctgtc catatattga ttattcttta
    19801 tttgaaagac tgatcaataa agtaagcttt tatgaagttt gtgaagaata tgatcttccg
    19861 tatcctaaaa cattgatcgt cagagaagaa atgttagtaa atggacattt ggaacaggaa
    19921 ttgccttttg aattcccagt tgctttgaaa cctgcaaaca gtgtggagta tctttctgtc
    19981 caatttgaag gaagaaaaaa agcttttatt ttagagacta gagaagagtt cgatttaatt
    20041 ttaggccgga tatacgaagc cggttataaa agtgaaatga ttgtacaaga ttttatcccg
    20101 ggtgatgata gtaatatgcg tgtgctaaat gcgtatgtag atgaagatca tcaagttcgg
    20161 atgatgtgtc ttggtcatcc actgttagaa gatcctacac cagcatcgat tggtaattac
    20221 gttgtgatca tgccagatta taacgaaaaa atctatcaaa ccattaaggc atttttggaa
    20281 aaaatcgaat atacaggctt tgccaatttc gatatgaagt atgatcctag agatggagaa
    20341 tataaattat ttgaaatcaa tttgagacaa ggtcgtagca gtttctttgt gacattgaac
    20401 ggcttgaatc tggctcgttt cgtcacagaa gatcgagtgt ttaacaaacc ctttgttgaa
    20461 acgacctatg gaacgaatca atcagataaa gcaagattat ggatgggtgt gcccaaaaaa
    20521 atctttttag aatatgctcg ggaaaatgaa gacaaaaaac tagctgagca aatgatcaaa
    20581 gaaaatcgtt acggtactac tgtgttttat gaaaaagacc ggtcaatcaa gcgctggttg
    20641 ttgatgaaat atatgttcca taactatatt ccaagattca aaaagtattt tcatgtgaaa
    20701 gaaggctagt tcatggagaa ttttttcagt attttaggcg gaatgggcac gatggcgaca
    20761 gaaagttttg ttcgcttgat caaccaccga acgaaagcta caaaagatca agaatatttg
    20821 aattatgtct tattcaatca tgcaacagtt cctgatcgta cagcttatat tttagatcga
    20881 tcagaagaaa atccaatgcc gtttctgctg gatgatattg agaaacaaaa tttattgcag
    20941 ccgaacttca ttgtgttgac ttgtaacacc gcacactact tttttgaaga attacaagcg
    21001 gcaacggata ttcctatcct tcatatgcca agagaagcag caaatgaact tgtccgtcag
    21061 catacgacag ggagagtagc gattctagga acagaaggaa gtatgaaagc tggaatctat
    21121 gaacgcgaag tcaaaaatct tggattcgaa acagtgattc ctgatactgt tcttcaagaa
    21181 aaaatcaatt atctgatcta tcatgagatc aaggaatcgg attatttgaa tcaagagcta
    21241 tattacgaaa tcttagaaga agcggtagag cgcctaaact gtgaaaaagt tatcttaggt
    21301 tgcacagaat tgtcattgat gcatgaattt gctgaagata accattatcc agtgatcgat
    21361 gcccaatcta ttttagcaga tcggacgatt gaacgagcgc tagctgaaag aagcaaggca
    21421 ttggatacag catcggaaaa gtaaaaaaat tggatttggg gcaaatatcc taaaagatat
    21481 ttggtccaaa tctttttgta aaagaaaaag aaaactggta atgatacgcc cggaatgata
    21541 taattttact atgcacaatg tgagaggagt tcgtaattat gacagataat ttgcaattag
    21601 tcatcataac aggtatgagc ggtgcaggga aaacagtagc tatccaaagt tttgaagata
    21661 tgggctattt ttgtatcgat aatatgccgc ctcgattgat tccaaaattc tgggaattaa
    21721 tcaaagaatc aggcaaagtc acaaaaatgg ctttaggagt ggatttacgt tctcgttcct
    21781 tctttgaaga aatccaaaac atgctgatcg aaatcgaaaa tacgaacttc atcgacactt
    21841 ctatcttatt tttagatgct tcagacgaag aactagtggc tcgctacaaa gaaacaagac
    21901 gagcacatcc aatggcgatg gacggtttga tcactgaagg gatacgtaaa gaacgggcga
    21961 tactagacga cttgaaagcc caagcttccg tgatcattga tacgacgaca ttgacaccac
    22021 gacaattacg tgaaaaaatc aatgagtcat tcaaatcaaa gggagaccaa ggatttcgga
    22081 tcgaatttgt atcatttggt tttaaatacg ggttgccgat cgattcggat attgtgatgg
    22141 atgtgcgttt tttgcccaac cctcattata tcccggaact aagaccattg accggaaaag
    22201 atcagccagt ctatgattat gtgatgtcct ttgatgaaac gcaatcattc taccataaat
    22261 ttttggatct tctggaaact gtattgcctg gatacattaa agaaggaaaa agcagcttga
    22321 cagttgcaat cggctgtaca ggcggtcaac accgttctgt ggcattgact gaacgtttgg
    22381 caaatgcttt agggaaaaac tataaagtca atgtgacaca tcgggacaag gataaaagga
    22441 aagagacggt gaaccggtcg tgaaaatgaa aacctaccgg ataagaaaac caaagatcgt
    22501 agtggtcggc ggtggcactg gtctgccagt tatcctgaaa agtttgcgaa atcaaggagc
    22561 agatatcaca gcagtagtga ctgttgcaga tgatggggga agcagcggtg caatcagaga
    22621 atcgatcgct atggctcctc ctggtgactt gagaaatgtc ttagtggcgt tatcggatat
    22681 gccacaattc tatgaagata ttttccaata ccgtttcaaa aaggaagatc agtttttagc
    22741 caatcatacg atcggtaatc tgatcattgc cgcagtttcc gagatgcggg gaagcactta
    22801 tgaggcgatt caattattag caaaaatgat gcatgtagat ggacatgttt atccttcttc
    22861 agaaactcct ttgaccttgc atgctgtctt taaagacgga acgactgcag taggagaatc
    22921 taaaatcgcg atcgaccgta aaaccattga ccgtgtgtat gtctgcaaca caaatgataa
    22981 aagtgaagcc aaagcggctc gaaaagtcgt ttcagcaatt atggacgccg acatggtcgt
    23041 tttgggaccc ggaagtttat tcaccagtat ccttccaaat ttagtgatac cagaaatcgg
    23101 cgaagcaatg ctcaagacaa ctgctgaaac cgtctatatc tgtaatatca tgacccaaaa
    23161 aggggaaacg gaacatttca cagatgccga tcatatacgt gttttacaca agcatttgaa
    23221 tcagcctttt atcgatacag tattggtcaa tacggaaaaa gtgcctgaag attatatgga
    23281 cccagagatc tatgatgagt atctagtaca agtcgcacac gacttcaacg gattgagaaa
    23341 tgaaggatgt cgagtgattt ccaccgactt tttggaattg aaagatggtg gtgtcttcca
    23401 cgatggagac aaagtagtag aagaattgtt ccgtttagtt ttcggttcaa aatattaagc
    23461 aatttattgt tggagaaagg aggagaatag gatgtcattt gctgcagatg tgaagaaaga
    23521 actaactggt ttggaagttc atcgagaaca tgcgaaagcg gaattagctg ctttgctgcg
    23581 aatgaacggc tctttaggta ttgtgaacca gcagtttatt ttgaacgtcc aaacagaaaa
    23641 tgctgcaatt gccagacgta tctattctct tttgaaagat cattatcagg tacgaagtga
    23701 gctactcgtt cgtaaaaaaa tgaaattaaa aaagaacaat gtctatatcg tccggttgaa
    23761 gcaaggcaca aaagatgtgt tgatggatct tgatatcatg gatggtatga tgttcaatgg
    23821 acacgtttcg gacgagatta tgggaaattc gcaaaaaatg cgttcttact tacgtggtgc
    23881 ttttatggcc tcaggttcag tcaataatcc ggaaacgagc cgttatcatt tggaaatatt
    23941 ttctatttat gaacagcata accaagacat ttgcgatatg ttaaattatt acggtctaca
    24001 tgctcgaacg cttgaacggc gcaatggcta tatttcttat ttaaaaggag cagaaaaaat
    24061 tgctgatttc ttaacattga ttggtgcaac caactcaatg ctccgatttg aagatgttcg
    24121 aatcgtacgg gatatgagaa attcggtcaa tcgtctagtc aattgtgaga cagcaaatct
    24181 gaacaagacg attgatgcgg cttctaaaca aatcgaaaac atcgaattga tcgaagcaaa
    24241 ggtcggtcta catgcccttc ctgaaaaact tcaggaaatc gcagaacttc ggcttcaaca
    24301 tccagaggtc agcttgaaag aattagggga aatgattccc tcaggtgcga tatcaaaatc
    24361 gggaattaat caccgaattc gcaaaataaa tgaatttgcg gaaaaactgc gtgaagaagc
    24421 agtttaatcc aaagaaaaaa ctgatacatg aagcaggaga tgcgaatgta tcagtttttt
    24481 ttaaaagtta tacttgcttt tcttccgtca aactataatt ccatctttac tggagtaaca
    24541 taatttggaa aggcttctat tgtttttaat acaatcgtat aaaaagcggg gagacaactg
    24601 aatgaagatt ttagttttgt acataaattt ttgcttaata tagaagtcct gatatagata
    24661 agatcaagta tttatctata actggcctga ttaattaaaa aactggaagg aactgctcga
    24721 tcatccttga gaactgctct tcttacttct ggtcaagatt ctcgtggttg gtggataagg
    24781 atggttgata agttcattgg attagctagt cgaaaacaac caggtgtaat taatgtagaa
    24841 gttttaacaa ggggatggag tgatttattc agtgataatc ataagttcat aaatagtaat
    24901 tgagatgtat ggttggaata cgttatacct gaacttgaac taccaagatt tggtttagaa
    24961 tttgagaatc gatgtttata aagggagggc aaaaatgcgt ttttttacaa cttttactat
    25021 cattatgatt gcagtacttt ttatttttct agatatagca aagcgaaata cagcattttt
    25081 attatatcgg gttttacttc gagcaggtct cataacattt atatcgatcg ttggattttt
    25141 tctattcaca gttattgtat ttatttggag aactccagca ccaccattgc cagagattac
    25201 atatggagaa tttcctttta gacttgagta tgaattgaat gaagaattac atgtgataga
    25261 agacacgtta atagttgaat ttgatggatt tggaatgaac gaaggcatag ggagatatcg
    25321 tagatggaca agtcgtttag ccagcggaga agatttagtg ttgctgttag aagttagtga
    25381 taataagcaa atattttatt ttccaggacc tgctaattat tacatgggag ataggttgaa
    25441 tggttataat catacttttc ctagtgcttc tttcattgaa agggaaagag gcattactag
    25501 aagggatatt ttacatgaca aagaattatt agaacaattt ggtccgcttg atcaaaatac
    25561 aataaatgaa gaagaattgt taaatcaata taatataagg ctagtgaatt gggaaatatc
    25621 tgaaccgatt gttaacaatt ttggtgatta gaaaacaatt acgataaata gaaacaagcc
    25681 aaatcatcag caagtaaaag ctgatgattt ggcctttctt atacgagtag taggaattca
    25741 tagcgaatat tattacgatc agtagatatt ttgatattaa aggagtatta cgaactagta
    25801 tgtgagaatg tagaggtgat ttgcgaataa tgagaaattt atcaaataga aacaaaatat
    25861 taatcattat tgttgttata gctgtgtttc atctgggaac gaatgcggtt ttaagtagaa
    25921 taatattggg tcctaagcca ccaagacctg cgattacccg aggggagttt gattttagat
    25981 tagaatatga agtagatggg gaaaggattg tgatagaaga tacaattgtt gcattatttg
    26041 atggattttc tgcagatgca ggtagcatgg catggtatcg cacgtggcgt ttgcatttgg
    26101 cgagtgacaa gagggcacaa caattattgt tacatgagtt agatgatggt cgaaaaatat
    26161 attatcattt tggaggagct ggattttata tggggaagtt agtagcagtg taactgtaac
    26221 aaggaaagat ttgccaggtc ttggcattcg aaactccata ttttatatcg agcgtgggag
    26281 agatacatct attgggaaca ggaaagggtt aactcttgaa gagttatata atgactttgg
    26341 aatccgtctt gttagttggg aacacaatcc accaatagaa aatcgctttg aataatcgaa
    26401 atctattaat ccatttcatc agccagtgaa tgttgtaatg gaacaatttg gtttagtgtt
    26461 caaaaccatt gaaatactag gctgtgatca gcaaaaatgt atctgaataa aaaagagagc
    26521 aactattaaa tgagaggagt taggagttgt tagctttata cgaatcaaag tggcaaatac
    26581 agatgcgtat gcatttgtgt ttgccatctt gcgtttggta gacgagatag gtataagcta
    26641 aagaaacaac ttgtttaaaa taaacgacct agctcaacaa acgaccatct aatcaactaa
    26701 cagcatacat aaaagcaact atttttgaag tagaattgaa atcgggataa cttaaaaagt
    26761 gattgggata ctagtgtgaa tcaccgtaca taaatattac tcgttaaggg aatttgagaa
    26821 attttgtatg aaaatttaag aattagttag tatgcgcaag tcaaaaatat gaataaatta
    26881 tttggtggag ataatttgct cagcttgtca taagaaatga gataatctgg cagatgcaag
    26941 cttttggaca ccatttattc ggaataagga ggctgggaca gaagcgttta actccaagaa
    27001 ataagaagga gttgcttctg cttccgccgt ttataggtgt ttagagcgtg agacaaaagt
    27061 gttctttact tttgtcctac gctccttatt tttttatttt gtttggatca attcttttaa
    27121 ttcctctatt gaaatagatt catcaaagat ttctttaccc aagatgacag tcggaacaaa
    27181 tcgaatatgt gcttgctcag cttcgttgat gacagcttgg ctcagctgtt cgtctttttg
    27241 ttctgtcagc tttagtttat ctactgcaaa atccgctact tcttggagag aaagatgttt
    27301 ccactcgtct tgagtatcga atatttgttt gatctctttg atcgcttttt gaccatcgct
    27361 gattgtgaga taccggtgca tcacgtttcc tcgctgcaag ctttcttttt ctttatcgaa
    27421 caatttgatc actcgctgca attgcccgct ttggactgct tcttctaata gctcataaga
    27481 ttcttcaaac cattgtcggc aatacggaca agataggttg atgaattcga ccattttttt
    27541 aggagcattc gatgaaccat aaaggattcc tttttgtgta tttgttttcg ttgcatcaat
    27601 cactgatata tccatgttaa aacacctctt tcgcttatag tttatacatg tctgacaaaa
    27661 aaaggcaacg atttagactg tttgtctaaa aattagatga ccaattcaaa tagacatatg
    27721 tgaaatattt ttataaataa aagaaaaaat ggaatgtttt acgttataat ggaacagata
    27781 ataagagaga atatgaacaa ggatgatgaa tcatgcgaaa aatgaaaaca atggatggca
    27841 atacggcagc cgcatatatt tcttatgcat ttaccgagat tgctgcaata tatccaataa
    27901 cacccagttc gaccatggct gaattagtag atcaatggtc agcagcagga aaagaaaatg
    27961 tattcggtca accagtaaaa gtagtagaaa tgcaatccga agcaggagca gctggtgtgg
    28021 tccatggatc attgaaaact ggtgcactga caactacata taccgcttct caagggctgc
    28081 ttttgatgat accgaatatg tacaagattg ctggggaact actgccaagt gttttccatg
    28141 ttgctagtcg ggcagtgacg acaaatgcgt tgaatatttt cggagatcac ggagacgtga
    28201 tggctgcaag acaaacagga tttgctatgt tagctgaaag cagtgtacaa gaagtgatgg
    28261 atttagcacc tgttgcccat cttgcttcta ttgaaggaga agtacctttt ttgaatttct
    28321 ttgatggatt ccgtacgagt cacgagattc aaaaaatcga agttttggaa tatgaagaat
    28381 tagctcctct gatcaatcag gagaaattag cttcatttcg taaacgaggg atgaatccca
    28441 atcatccgac tgttagtggt acgaaccaga atccagacat ccattttcaa caacgggaaa
    28501 cgatcaaccc ctattatgat aaattacctg aaatcgttca aaaatatatg aaagagatca
    28561 accgattacg aggaacttct tatgacttgg tgacttatta cggagcagac gatgcggaag
    28621 aagtcattgt ttctatggga tcagttgcac agacgattga acaaacgatt gattacttga
    28681 atgcaaacgg acggaaaaca ggttttttga atatccatct ttatcgtcct tttccagtgg
    28741 aaacattcgt gcaaaagttg cctcatacag taaaaacaat tgctgttctt gatcgaagta
    28801 aagaaccagg ggcaggtgga gaacctctct tattagacgt tcaaagtgcg ttatatgatt
    28861 ctgaacttcg cccggctgtt attggtggtc gctacggatt ggggtctaaa gatgtgactc
    28921 ccgaccaaat ctcagcagtt tttgatgaat tgaaaaaaga cccaagcata cgcaaaaaac
    28981 ggtttaccat agggatcgtg gatgatgtga cttatcaatc tttggaacca aaggaaagtc
    29041 tggatttgac agaaccgcaa acattccagg ccaagttttg gggatttgga tctgacggaa
    29101 cagttggagc aaataaatca gctatcaaga ttattggaga tcacaccgat aaatatgcgc
    29161 aaggctattt ttattatgat tcgaaaaaat ctggcggtct gactgtctct catcttcgtt
    29221 ttggagatac gccgatacgt tcagcgtatc ttgtcgagca tgcggacctt gttgcctgtc
    29281 acacaccagc ttacctgcat agctatgact tggtcaaagg attgaagcca ggtggcattt
    29341 ttttattgaa tactctttgg agcgacgaac aattagaaac gcatctgccc ttaaaattaa
    29401 aacgttactt ggctgaaaat aacattagat tctatacgat caatgccatg agattggccc
    29461 aagaagtcgg tttgggacgc cggatcaata cggcaatgga aacggctttt tttaaattag
    29521 ctgacatcat tccttttgat gaggtgcttc cgctgctaaa agaagaagcc ctgaaaagct
    29581 atggacataa atcaatggct atcgtagaaa aaaacataca agcaatcgat cggacgaccg
    29641 aactgcttca tcaagtcgcg atcccagacc attggagaac gttggagatg cctgaaaaaa
    29701 tcaataaaag gaacactggt tatatacaag aaatacttga accagtgaat gcacaggaag
    29761 gaaatcaact ctctgttggt acacttgtag caaatggaat gacaactggc gaaatgcctt
    29821 taggaacggc ttctatggaa aagcggggcg tcgctttaga agtacctgag tggattagtg
    29881 atcgctgtac gatgtgtaat gaatgtgcct ttgtttgtcc tcatgctgcc atccgtccat
    29941 ttttagcaga tgaagaggaa atggaagaag cgccagaagg atttatcgtg agagaaatgc
    30001 gaggcgcaga cggcgtaaaa taccgtatcc aagtttcagt ggaagactgt acaggttgtg
    30061 gtctgtgtgt agaggcttgt cccgcaaaag aaaaagcttt agtgatgaag ccttatgaag
    30121 aacagaaaga acaagctgtg aattgggctt ttgcaatgac attaagacaa aaagcaaatc
    30181 cagtcaaagg aaagaatact gtcttgtcta cacaattcaa tcaaccgctt cttgaattct
    30241 cgggcgcttg ctcaggctgt ggcgaaacac cttatgtaaa attattgaca caaatgttcg
    30301 gtgatcgaat gatgatcgcg aatgcgacag gctgttcttc tatttggggc ggtgcttctc
    30361 ctgcttctcc ttatacgacc aatgaatgtg gtcaagggcc tgcatggtca aattctttat
    30421 tggaagataa tgctgaattt ggttatggta tgcttctagc ggcacagact cgtcgcgagg
    30481 ctttggcaag ttcaatcaaa gaagtattgc caaaagtatc agaagaacta cgagaattga
    30541 tggaagactg gttggtacac atgtttgaga gcgaaggaac gcagcaaaga gcagcaaaac
    30601 tgcaagctgc gatcgaaagc gaacgatcaa ttgtccctga actggaggat atatacaaga
    30661 gaaaggatct attcgtaaaa aacagccaat ggatgattgg tggagacgga tgggcttacg
    30721 acattggttt tggcgggatc gatcatgtac ttgccagcgg agcagacgtg aatattcttg
    30781 ttttggataa cgaagtctat tccaatactg gcggtcaaac ctcaaaagca acaccagctt
    30841 cggcaatcgc taaattttcg gctagtggaa agtatgcttc aaagaaagat ctaggaatga
    30901 tggcgatgac atatggaaat gtttatgtgg cgcaaattgc ttcaggtgcc aatcaaatgc
    30961 agacgataaa ggcttttgaa gaagcagaaa agtatcctgg accttcttta atcattgcct
    31021 atacgccttg tatcacacat ggtttgcttg gtggaatgaa agaatcgctt aaagaagcaa
    31081 aagaagctgt ttcttctggc tattggtcat tatatcgata taaccctgca atggtcgaaa
    31141 aaaacaagaa cccaatgact ttggactaca aaaaacctga tttctctgct atgcctgatt
    31201 tcatgaggaa acaagttcgc ttttcctcgc tagagaacgc aaatccaaat cttgcaaaag
    31261 ctttatttga aaagactatc caagacgcta aaacacgttt ttacaactat gctgcactga
    31321 cgggagaatt agaaaaaatc aagaaacgac ttgaaccaga ggaatctatc gatgtacccg
    31381 ttaagactag aaaagaaaaa acgacggacc ttgaagcaga agaaagaaga gctgccagag
    31441 cagaacgacg taaaaaaatg gaacaagcta caaaagaaaa aaaggaaaaa taacagaaaa
    31501 aatataatga ataattctct gaagtttaaa aatatttatt gcagagaaaa ataaaacgca
    31561 tacttgcaat tttcctgaaa attatgcgtt tttctcaaga aaaatacgag cagatcagcc
    31621 tctaaaatta aaacatatga tatactagag aggaaatctc aaaagagaag agaggctttt
    31681 tatgtcattt caaatcgggg aactttttaa tttggactat tggcagcaaa tgttttccgg
    31741 tgacttgttc tctctgaatt ttttggtgaa catacttgat attctggttg tctggtatct
    31801 tgtatacaaa ctgatccaac tgctgcgagg cacaaaagcg attcagcttc tgaaaggtgt
    31861 agctattttt attgttatcc gaatcgtagc ggaaatcatt ggtctgcaca ccttgtcttg
    31921 gctgatgaac caagtcatta cgtacggggt gatagctgct atcgtgattt ttcagccgga
    31981 aatccggaga ggactggaac atcttggacg cagttcgctt ttcaaacaat caaaaagcga
    32041 acagcaggaa gatgagaaga tgatcttgtc ttttgacaaa gccatccaat atatgtccaa
    32101 gcgaaaaatc ggtgcgctaa ttactatcga acggcatact ggattagatg agtatattga
    32161 gacaggaatt gcattagatg cagatatcac tggagagctg ctgatcaata tctttattcc
    32221 taatacaccg ctgcatgacg gtgcagttat tgtaaaagaa ggaaagatcg cagtagcaag
    32281 cgcctatctt cctctatcag aaagtatgtt gattccaaaa gaattcggga ctcgacaccg
    32341 ggcagctgtc ggtataagcg aagtcagcga cgcgattacc atcgttgtct ctgaagaaac
    32401 tggggatgtc agcattacat tagataatga actgatggct ggcttatccc agcaagaata
    32461 tttggcaatt ttgcgtagag aactgattgc tgaagagaaa acaaaaaaca agaaaaacgt
    32521 gattcaatct ttcctagacg gtatgactaa gggaggggat cgcaaatgat ctcaaaagag
    32581 aaacggacaa gcattcttta cagttttatt gcattgctgt tcagtattgc gctgtacttc
    32641 aatgcaaatg gccagtccgt tccaaatacg ctttctggaa atgaagcgta taatcaaaca
    32701 gttactgggg tgccaatcaa gatttcttat gattcaaagc gctactatat ccatggatat
    32761 gaaaatgcag tgactgtcaa actttccagt gcaaaccgag ttcagttgaa tgcagaggca
    32821 aacgaagata cacggatgtt ccgcgtcaca gcagatatca ctaagctggg cgaaggaaca
    32881 catgaggttc cgctaaaaat tcaaaatttg agcagtgctg tcacagcaaa gatcgaacca
    32941 tcgactatta cagtaacggt tgagaaaaag gtaataaaag atttcgatgt ggaaacgttg
    33001 attccttcaa cgttcacacc atctggatat gaacttgatg atacttccgt tagtcctaaa
    33061 aaagtctcta tcactacagg tgacaaaaca ttggcagaaa tcaaacgagt agtagctgat
    33121 gtggacgctt cgatggtcac agatgatggg attaatagtg aggtcccaat acaggcgctg
    33181 aatgcagcag gggaagtatt aagtatcgtc tctgatccag tgcaagtaac agtgagagct
    33241 gattcgatca agccaagcaa gtcggtacga ctttatggga tacagcaagg aacaccagct
    33301 gccggggtag aaagctatga tttcagtttt tcaaaactgg aagcagaagt aagcggctct
    33361 agcgatttgc ttgcatcgat cggtgacagt attgctgtac cgatcaatgt ctcaggtatc
    33421 aatcatcgaa caactcgtaa aattgaaata ccagtagaag aggggttaag tatcaagccc
    33481 gaatcagtta gtgtggagat tacacccgtg ttgcaaacgt caagcagtag ctctgcttcg
    33541 acgagcagtc ggacacaaga aaatacaagt acgagtagaa gtatttcatc tgctgattca
    33601 ggaacggaac catcgacaac aaccatttct tccagttcgg aatctgggac aagcagctcg
    33661 accacaagta cagaagaaac atcagaaaca agcgaatcaa cacaaatgag cagaacaaat
    33721 tcctcagaaa attaagttaa aggagtaatt ttacaactat gggtaaatat tttggaacag
    33781 acggcgtacg cggtgaagcc aacaaggaac ttacgccaga acttgcattt aagctgggac
    33841 ggtgcggagg ttatgtccta agccagcatg aagaagaagg aaaacgccca cgcgtgttag
    33901 tagggcggga tacaagaatc tcaggtcaaa tgttagaaga agcgctgatt tcaggacttt
    33961 tatcggtggg tatcgaagtg ttccagctag gtgtgatctc tacaccaggt gttgcttatc
    34021 tgacacgttt gcaaaaagca agcgcagggg tcatgatttc tgcttctcat aatccagcac
    34081 aagataatgg aatcaaattt ttcggtgcag atggttttaa actagtagat gaccaagagc
    34141 tagaaattga agcattatta gatgctgaag tggatgaatt gcctcgtcca tcagctgaag
    34201 gattgggaac tgtagaagaa tttccagaag gcttattgaa atattctcaa ttccttgtac
    34261 aaacgatcaa cggtgacctt tccggtttga ccatttgtgt cgatgcagca aacggagcaa
    34321 cttcaacaag tgttaatcgt ttgtttgccg atcttgaaac agatttttat accatgggca
    34381 caaatccaga cgggttaaat atcaatgatg gcgtaggctc tacccatcca gaacgtttag
    34441 ctgagatggt cgttgaaaaa ggagcagatg ctggactagc tttcgatggc gatggcgatc
    34501 gtattattgc ggtggatgaa cttggaaaca ttgtcgacgg tgataaaatc atgtttatct
    34561 gtgctaaata cttggcagaa aaaaatcgat tgaaaaaaga tacgatcgtt acgactgtca
    34621 tgagtaactt aggattccac aaagcggtgg aaggaatcgg tttgaaagat gtcgttacac
    34681 aagttggtga tcgttacgtt gtagaagaaa tgcgcaaaaa cgattataac tttggcggcg
    34741 agcagtctgg gcacatgatt ttcttagatt ttaacacaac tggagatggg atgctttcag
    34801 gaattcagtt actgaatatc atgaaacaga caggtaagaa attatcagag cttgcagcag
    34861 aggtaacaat ctatccgcaa aaattagtga atatccgagt aagtgataaa aacggtgcga
    34921 tggatgttcc ggcaatcaaa aaagtgattg aagaggcaga agctgaaatg ggatcagaag
    34981 gaagaatcct agttcgtcct tccggtacag aaccattact tcgagtgatg gcagaagcgc
    35041 ctactgatga gaaagttaat tactatgtcg ataaaatcgc ggcagtagtc aaagaagaaa
    35101 tcggactgga ttaagagata ttttcataaa aaataaaaga acaaggacca gtgtcagatg
    35161 tctgacactg gtccttgttc ttttattatt ctactgtcac tgatttcgct aaattacgtg
    35221 gtttgtcaac gtcgaatccc cgtaatagcg ttgcgtaata tgcaagtaat tgagtcggta
    35281 cgactgtggt tagagtagtt agataagggt ggacagttgg tagcaccagc tggtcacttt
    35341 ccttttctaa tccctctgag acaataacta gtgttttagc gccacggctt tcgacttctt
    35401 tcaggtttcc tcgagtatgc gcaccagtca cttcttcact gatgattcca atcactggtg
    35461 ttccttcttc gatcaaagca atcgttccat gtttcaattc gccagcggca aaaccttctg
    35521 cctggatata actgatttct ttcaatttca aagcagcttc aagagaaacg ttgtagtcag
    35581 cgccacgtcc aatgtaaaag gcatttcttg tatcgctgag atatacttct gccaactctt
    35641 ctaaataatc tttttgggcg atgaccgatt ccatcgcatt agcaactaaa ctcaattcat
    35701 gagtgatatc gaatacaaga gaagtttcaa attctttttt atcaccgata gctttggcta
    35761 ataaagttag tacagctatt tgtgcagtat aagctttcgt tgatgctact gcgatttcgg
    35821 gtccagcatg caatagcagt gtaaaggatg cttcccgcga aagagtagaa ccagcaacat
    35881 ttgtaatcgt aagtgaaggg tagccgagac ggttgatttt taccagaact tggcggctat
    35941 ctgctgtttc gccactttga ctcaagaaaa taaagaatgg tttttttgaa agaagaggtg
    36001 tgttatagcc aaattcactg gctaaatgaa cctcaacagg gatctgtgtc aatttttcca
    36061 gagtctgttt acctgctaac cccgcatggt aactagttcc gcaagcaacg atatagattc
    36121 gatcgctatc agacaatgta tcgaccagct tttgatcaag agagacacga ttgtcttctg
    36181 ttacgtattt ttgagcgata cgacgcatga taataggctg ttcgtcgatt tctttcagca
    36241 tataaaaagg ataggtacct ttctcgatat ctgaagaatc tacttgtgct tcgaatgaag
    36301 aacgttctac gatgtcacct gcaaaatttt gaataataac tttttctgat gtgacggtaa
    36361 ccatctcttc atcttcaatc tcaacgaatt ccttcgtatg agcaagcata gccattgcat
    36421 cgctggcaat cacattgaat ccatcgccga gtccgattaa tagtggactt ttatttttag
    36481 cgacgtaaat aacatcaggt gctgtccgat caatcaaagc gaaagcgtaa gaacctttga
    36541 tgacccgcaa agcttttaaa aacgcctctt gcgttgtagt atccttcgcg aaagtttcaa
    36601 ttagatgagc gataatctca gtatcggtct caccaataaa gtggtcattt gctaaataag
    36661 caattttaag ttcttcaaag ttttcgatta cgccattgtg aacgagcacg aagcgtccac
    36721 tttgggaagt atgtggatgg gcgttttctt cagaaggttg cccgtgagtt gcccagcgtg
    36781 tatgaccaat acctatattt cctgtagtat caatatttat tttgtttttc aaattttgaa
    36841 ttctgccttg tgctttgacc agataatcag ttgtgccatc tgagacaaag ataccagctg
    36901 aatcataacc acggtattct aatttttcta gaccgtcaat caatcctggt gttacatttt
    36961 ttaatccaat cattccaaca attccgcaca taaaaaacat cctactttct ataatgaatt
    37021 cgtatgaatg cttccagctg caggaaaatc cttttgtttt atctttgcag atattttttg
    37081 tggattttct gtagagtgca gtccctaggg ccatccgccg aatctttcga taaaccctct
    37141 cctcgtcatc tgtttacaca gacctggcgc tatcctaaaa ggtataacta tcctcctctt
    37201 ttgtttttta tctggaaaca tttaaaatca taaccttttg cgaatgttct tgtcaacaaa
    37261 aaaagaataa aaaataaatt ggtatatatc aaatgctttg tcaaatgctc tttttatgtc
    37321 aaaaaataat gggtttttat ctatcatctt tttcaggagt tcggtacaat agaaagtaat
    37381 cggatagagg aggagtaaaa atggattcaa aaattgagat catgacactg ggaatgttaa
    37441 aaaaacaatt gtccaaattc gaagcatcag ctggtgtatc agatgatacg aaaatatttt
    37501 tagatactgg ctgggatagc atccaagaaa tcgcacctga tgccttggaa gttgctgaag
    37561 ctcgtgagtt tacggtagaa gatgaattga caaaagagtc gttttccggg tatgcgcggg
    37621 aagaaaaagc agagcggttc gacacttctg agcagtcaga aactgtgatc gtcataaaaa
    37681 acttgtatta gaaaggagaa agaatgaata agagacgttg ggcagcagtt ctgattgctg
    37741 caggattatt tgtggtttcg ttagtttcag ctagtttgac taagcctcag gaaaacgatc
    37801 aaattgaggg attgaacagt tggttatatg gggatgaaga actagcttca aatgtgttgg
    37861 aagagggaga cagtacgcaa aaaatcgtaa aattatcagt agatggaacg attgctgcgg
    37921 gaggggcttc aggtttattt gcttctgaag gttacaatca taaaaatttt atggaacaac
    37981 tgaaggcgat tgaagaagat ccaacagtca aaggtatctt tttagaagta aattctcctg
    38041 gtggcggtgt ctacgaaagt gcggaaatcg ctaagaaatt ggatacgatt cgtaaagaac
    38101 atgatatccc aatgtatgtc agcatgaaaa acatggcagc tagcggtggc tactatattt
    38161 ctgctcaagc agataaaatc tttgcaacag aagaaacagt gaccggctca attggtgtga
    38221 tcatgtcagg attgaattat tcaggacttc tagaaaaact gggtgtggaa gatacaacag
    38281 ttaaaagcgg agcgttgaaa gatatgggtt caggaacgcg accagagaca aaagaagaag
    38341 aagcggtgct tcaagcatat atcgacagcg catatagtcg ttttgtagca atcgtcagcg
    38401 agggacggaa taaatctgaa gaagaagtca aaaaaattgc tgatggaagg atttatgatg
    38461 gtgtccaagc aaaagaagta ggtttagtcg atgaattggg atttcctgaa gatgctttaa
    38521 aagctatgcg aaaggaacaa cagcttgaag atgcggaact agtagaatat tcttccacgc
    38581 catctgggtt tgctagtaca tggttaggaa gtaaactagc agaattccag ggattgaagc
    38641 cttctgagac tagtcagatc ttatccgttc ttgagaatct agggacagcg gaatcaccaa
    38701 aagcaatgta ttattacgga ggtgagtaag catgtcagag ctttcaaatg aaaataagga
    38761 acaagaaaaa gataagcaac agcagcctgc tgtaggacct gactccaact tttcccaaaa
    38821 ggtactgagc tcttttgaaa gagagccttt gacgcccgaa gaaatcaaaa agaaacaacg
    38881 taaatggaaa aagtatacgg acacaagaaa tgaaccgcca aaagcaaatg attttcctaa
    38941 gtatttctat gcaggttttt ttatccgaat gtttgctttc ttggtcgatc ttttgtgtat
    39001 cggagctatt acgcgtttaa cacttggtat tgccaaaaat ctaggttggc ttaatttttc
    39061 agattcttat ctgagtatct acggattcac agccttactt atttatctag cgtattttat
    39121 tttattgacg aaattcaatc atggtcaaac gattgggaaa atgatctttg gtattcgagt
    39181 catcagcttt gatgaagaag aacttagctg gtcaaccgtt ttagttagag aaggtgcttg
    39241 ccggttcatt ctaaaatttc ctttgctgat gttagggtat cttccagcaa tctttaatca
    39301 gaaaaagcaa catctaggcg attttttcac taatacaagt gttgttacga tcaatctgat
    39361 caaagcattc aaccaggaag tcaacagctg atgagcaata aaaagcagta acagttgaaa
    39421 gcaccttcta aaagtcagat ctaaaatcta gcttttagag ggtgcttcag ttatttttaa
    39481 ttgtcatgct ttttttgttt tgctactatt gaacatgagc tgaacatgag caagaggagg
    39541 acactgcgtg aagccgattg attttccaga aaaatatgag aatttaatgc gagtagcaca
    39601 acaagcacta gcaaaccagc aatatcagca ggcaaaagaa ttatttcaac gagcttacga
    39661 gttgaaagaa agctttgagg ctaatagcct gcttgttttt tgtctttacg agttggacga
    39721 gaaaaaagaa gcattgaagc aagcactctt gcatgaaaaa cagtatcttc aaaatgagga
    39781 gtttgctgaa ttttattttg acttgttaat cagtacacaa gattttttat atgctagaaa
    39841 attgatagca agtacagatt tttatgaagc tttcgagcag cgggtcattg aaaaaatcca
    39901 atttgcagaa gaattatccg gacaaatgga gaggcagaag atcaaggcac tccacaataa
    39961 atcggaggaa cttccttcgt tggaacctgc acgtcagctt gcgtctattg atgagatcga
    40021 acagcttcct tatcatgagt ttatccaaac agcgagtaaa cttattgtat cgccagaggt
    40081 ccatatttta gcaagggcaa aattactgga gacacttagg caattgaacg aatgccatcc
    40141 tgttttctat ttgacgattg aagaaaagtt agtcaaagtc ataccaaaag atctgccaaa
    40201 gccgcagcag caggcaagtt accagcaatt atgtgtattt tcagatcgtt acggtaatga
    40261 agatgcgctg cttagttccg tgttaaatga agaattcacg ttgcaatctg caattgttta
    40321 tcctgtctat gatacataca tcgaagaccc gaagcgctgg tttcaactga ctgtagaggc
    40381 gtgtacagga aaaacgatgt atgaaggtac agaagatgaa aaacaagatt ttttaaaaaa
    40441 aagagaaaaa attttgcagc aaatgatctt cttccattag attttatcag tgaaaaagat
    40501 aaaaatgtta tgaaaaacaa ggattctatc cattcaatat gtttacaatt tgaaagggtt
    40561 agtgtactat ttaaaggtat gcatgaaacg tttttgatgt ataaaatgaa tttctaaact
    40621 cggagggaat taaatgactg caacatttga aaaaaaaggc acgaatgatg gtgttttaaa
    40681 atttacgatt gcgcaagacg aaatccaaaa aggattaaca actgcgttta ataaagtgaa
    40741 aaaaagcttg aacgtacctg gattccgtaa agggaaagtt tctcgtcaag tattcaaccg
    40801 tatgtacggt gaagaagctt tatatgaaga tgcattgaat gctgtattac cagaagctta
    40861 tgaagctgct gtcaaagaag ctggcatcga tcctgtttct caaccaaaaa tcgatgttga
    40921 aagcatggaa aaaggtcaag actgggtaat cactgctgaa gtaacagtga aaccagaagt
    40981 aaaattagga caatataaag atctagttgt tgaaaaacaa gaccgtgaag taacagatgc
    41041 tgacgtggat gcacgtatca aacgtgaaca agaagcacaa gctgaattag ttatcaaaga
    41101 agatgctgca gctgaaaatg gcgatacagt agtaatcgac ttcgaaggct ttgtagatgg
    41161 cgaagcgttt gatggcggaa aaggcgaaaa ttactcactt gaattaggtt caggctcatt
    41221 cattccaggt tttgaagatc aattagttgg tcataaatca ggtgaaagtc tagatgtaaa
    41281 tgtaactttc ccagaagatt atcaagcaga agatcttgct ggaaaagaag cagtcttcaa
    41341 agtgacaatc catgaagtaa aagcaaaaga agtgcctgaa ttagatgacg aattcgcaaa
    41401 agatgttgat gacgaagtat caacattaga cgaattgaaa gaaaaatatc gcaaagaatt
    41461 agtagaaaca aaagaaaaag cagcggacga tgcaaaagat gaagcagcga tccgtatggc
    41521 tgttgaaaac gcagaaatcg ttgaattgcc acacgtgatg gttcatgatg aagtacatcg
    41581 ttcaatggac gaattcttga acaacatgca acgtcaaggt atctctcctg aaatgtacta
    41641 tcaattaact ggttctacag aagaagactt gcataaacaa ttcgaaggtg aagcagaaat
    41701 gcgcacaaga acaaatcttg tgatcgaagc aattgcagct gctgaaaatc ttcaagcaac
    41761 agaagaagaa atcaatgcag aaatccaaga attatcagaa acatacaaca tgccaatcga
    41821 acaaatcaaa cgcgtgttga cagaagatat gctggaacat gatgtgacaa tgaaaaaagc
    41881 aatcgaattg atcacaggta cagcgacaga agcgaaagaa tagaaatcaa gtaataaatc
    41941 atttctgaag aagctacttc tagaaaattg tttattcgaa ataagggact gtgacacgat
    42001 tttgtcacag ttccttattt tttgttaaga gtaaaaaatg caaaggtata ggtcagttga
    42061 tcctttgtta aacacttttt ctttttacta aactatgtta tgatagtaaa agactgtaaa
    42121 gtctttgata gtttcttgca tactttttgc aagttaaggg gtgaagacca tgtatgacaa
    42181 tccaagtagc aatgagaccg tacgctgttc tttttgcggg aaaacccaag aagaagtgaa
    42241 aaagatcgta gctggtccag gggtctacat ctgtaatgaa tgtatcgacc tatgtaaaga
    42301 aatcattgat gaagaatttt atgaagaagc tgtacgcgaa ttcactgaag tccctaaacc
    42361 actagaaatt cttgaagtat tgaataacta cgtgattgga caagatcgcg caaaacgctc
    42421 gttagctgtt gccgtataca atcactataa acgtgtcaat cagcaagcac aagaaaacac
    42481 agatgatgtt gaactgcaaa aaagcaatat ctgcttgatc ggacctacag ggtctggtaa
    42541 aacattttta gcacaaacat tagcaaaaac attgaatgta ccttttgcaa tcgcagatgc
    42601 gacaagtttg acagaagctg gttatgtggg ggaagatgta gaaaacattt tgcttaaatt
    42661 gcttcaatct gctgattaca atgtggaacg tgcagaaaaa gggatcatct atattgatga
    42721 gatcgataaa attgcgcgta aaagcgaaaa tgtatctatt actcgtgacg tttccggcga
    42781 aggtgtccaa caagccttgc tgaaaattct agaaggaact gttgcaagcg tgccacctca
    42841 gggaggacgt aaacatccac atcaagaatt tattcagatt gatacgacga acatattgtt
    42901 catcgtaggt ggtgcatttg atggcatcga aacgattgtg aagaatcgta tgggcgaaaa
    42961 gacgattgga tttggtacac aaaaccaaaa actttctgaa gacgaaagtg tcatgcagca
    43021 tatcattcct gaagatttac taaaattcgg attgatccct gagtttatcg gacgattacc
    43081 tgttatggca gctttagaga aattgacaac agatgatctg gtacggatct tgactgagcc
    43141 gaaaaatgcg ctagtcaaac aatatcaaaa acttctttct ctagataata ccgaacttga
    43201 attcgaacct gaagcgttga gagccattgc gaaaaaagca atcgaacgca ataccggtgc
    43261 tcgaggactt cgttccatca ttgaagaaat catgatggat gtgatgtttg atatcccttc
    43321 aaatgaaaac atcgaaaaag tgattatcac aaaagaagca gctgaattgt ctggtgagcc
    43381 aacagtaatc tataaagatt cagagaaaaa agcgggttga tgaaaagagg ctgccgtaaa
    43441 agtcattttg gcttttgcga cagccgcttg ctttattcat caccttgtta aggagagaga
    43501 ccatgaaagt ccatcaagct gaaattatca taagtgcagt ggcaccaaaa caatatccag
    43561 atacacaatt gccagaaata gctctggcag gacgatcaaa tgtagggaaa tcttctttta
    43621 tcaacacatt gattgatcga aagaatttag cccgtacttc cggtaagcca ggaaagaccc
    43681 agacattgaa tttttatttg atcgagaacg cattacattt tgtcgatgtt ccgggatatg
    43741 ggtatgcaaa agtatccaaa actgagcgag caaaatgggg aaagatgatc gagacatatt
    43801 taacgactag agaccagcta cgtgcggtcg tttctttagt agatttgcgt cataagcctt
    43861 ctcaagaaga tatccagatg tatcaatttt tgaagtatta tgatatccct gtaattgttg
    43921 ttgccactaa ggcagataag atccctcgtg gtaaatggaa caagcatgag agcgaaatca
    43981 aaaaagcgtt ggattttgat cgcaatgaca cgtttattct cttttcttcc gttacaaaac
    44041 agggaaaaga agaagcgtgg gcagcaatcg aacaagcaat tgaatcataa aaagaataaa
    44101 aaagacttac cagtcaaact ctttcataaa agtttggctg gtaagtcttt ttttgagctt
    44161 aacttacttt tgtttcttgt cttttttctt ttcattttgt ggctgtggtt ttagaaatcg
    44221 atcgatctct tcttttggtg caactgtttt ttccggatcg acagcaaatg attcgaaaag
    44281 tttatctaaa ttatctgtac gttcaaattt caaccccatt gtaaacacct ctttgtttct
    44341 ttttacttag tctatcatgt aatcgtccaa attggtttct aagacataaa gttttagttt
    44401 ttctttatcg ttggttataa ttaaattata aaaagatttt aaagaggtga gaaaatgatt
    44461 ggcgaagata aattattgat gactatccag ctcacagact taaatgaaga agaaaaaaaa
    44521 caggaaataa gcgattgggc tattttgaca cgtctgttga tccagccaac atttatccgc
    44581 tctttcacac aacaagcgga tccgtatgat cttcgaaagc ttcaagaaaa aataatgcta
    44641 gcatcggtaa gagacgaaag ttggttagtt gtaggaaatg acgagaacga atgtagtttt
    44701 cgcttggaag ataatcaatt attgataaaa aatgtattgt ctatctctgt tttcaaagaa
    44761 aagcaatttt tgattcgaga cttcattcag aaaaaaatga ttgctcatgg ggtgttcgcc
    44821 tatatgcgag cttatagcga gtttatctat cacaatacaa aacagatctc tcaacggcta
    44881 ttgtttgaga aaaaggatga aatcgaacat ttacctaaaa tgaaacaaca aaacggggaa
    44941 gtagtagtcg attgcaacca atttccagga tacgatcttt tttaccaagg tttatgtttc
    45001 acttcgtgtt gggaaatgta ttatagtcgc tattatcatc aaatcatccc taaaccgatt
    45061 tttctcgaca tccaacaagt agaaaaagtg aaggaattag aaaacgaagt catatgtatc
    45121 cagctttatc gagacccctt taactggcaa aagccaaaca atcaaatgtt tcaatcctat
    45181 tttagggacc agcttgggtt tgatcatctt gcttgggaca atggtgtcgg tttattgaaa
    45241 ccgccattcg ttgaatacat ttacactgac catacgatcc aatctgtcca gtatcagaat
    45301 gcacaaatgc aacctgttcc taaaaagaat gcttcatttt ttgtaacaaa aagttatgat
    45361 attcaacaag gagattataa agaaaggcgt gttcgcggaa cactaaatgc tcaggcgtat
    45421 tttccttggg ttgacgagaa tcgctcgcgc atgatgtgct ataaagttat cgatccgact
    45481 gttgctttgg ataatggaat cgaagcttat tgttattata ttagggaata tctagaagta
    45541 gaggtaacag atgaaaaata tcaagagtat cttttatcct taagaatcta tgttccttca
    45601 gaatccctgt cagaattacc gttaaaggaa atcaaacata gactctctga tgttaccttc
    45661 aaacgaataa agaaaaagcg gcgaagtatc caattcgatg tcaagaaagg tgagcagcat
    45721 ctgcgtgtgc aatttttaga ttaccgagaa ttagaacaat tgattacttt acaaaaaata
    45781 tgaggtgaag aaaatggata aacaactcta ttatcgaata atggttccgg catcattatt
    45841 atccgtctgt ctcttttttg ttcctaattt tcctattcga tcagtcatcg gttttttggt
    45901 catgtttggc gggatgggac tttattat
//
LOCUS       DAZUMT010000008        44927 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000008 DAZUMT010000000
VERSION     DAZUMT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.008"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8FM_000413"
     CDS             212..604
                     /locus_tag="ACG8FM_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5093104.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8FM_000414"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8FM_000414"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS5093105.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8FM_000415"
     CDS             2698..2943
                     /locus_tag="ACG8FM_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093106.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8FM_000416"
     CDS             3165..4775
                     /locus_tag="ACG8FM_000416"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS5093107.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8FM_000417"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8FM_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093108.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8FM_000418"
     CDS             5067..5936
                     /locus_tag="ACG8FM_000418"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS5093109.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8FM_000419"
     CDS             6101..7372
                     /locus_tag="ACG8FM_000419"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5093110.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8FM_000420"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8FM_000420"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS5093111.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8FM_000421"
     CDS             8763..9026
                     /locus_tag="ACG8FM_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS5093112.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8FM_000422"
     CDS             9471..9920
                     /locus_tag="ACG8FM_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5093113.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8FM_000423"
     CDS             9977..11395
                     /locus_tag="ACG8FM_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5093114.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8FM_000424"
     CDS             11395..12255
                     /locus_tag="ACG8FM_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5093115.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8FM_000425"
     CDS             12429..13202
                     /locus_tag="ACG8FM_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5093116.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8FM_000426"
     CDS             13349..13714
                     /locus_tag="ACG8FM_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS5093117.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8FM_000427"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8FM_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5093118.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8FM_000428"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8FM_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093119.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8FM_000429"
     CDS             15560..16216
                     /locus_tag="ACG8FM_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5093120.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8FM_000430"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8FM_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS5093121.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8FM_000431"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8FM_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS5093122.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8FM_000432"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8FM_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5093123.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8FM_000433"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8FM_000433"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS5093124.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8FM_000434"
     CDS             21888..22202
                     /locus_tag="ACG8FM_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS5093125.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8FM_000435"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8FM_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS5093126.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8FM_000436"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8FM_000436"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS5093127.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8FM_000437"
     CDS             23546..23875
                     /locus_tag="ACG8FM_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS5093128.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8FM_000438"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8FM_000438"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS5093129.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8FM_000439"
     CDS             24863..25687
                     /locus_tag="ACG8FM_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS5093130.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8FM_000440"
     CDS             25680..26027
                     /locus_tag="ACG8FM_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS5093131.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8FM_000441"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8FM_000441"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS5093132.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8FM_000442"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8FM_000442"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS5093133.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8FM_000443"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8FM_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS5093134.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8FM_000444"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8FM_000444"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS5093135.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8FM_000445"
     CDS             31684..32451
                     /locus_tag="ACG8FM_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093136.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8FM_000446"
     CDS             32441..34483
                     /locus_tag="ACG8FM_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093137.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8FM_000447"
     CDS             34764..34913
                     /locus_tag="ACG8FM_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS5093138.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8FM_000448"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8FM_000448"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS5093139.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8FM_000449"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8FM_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS5093140.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8FM_000450"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8FM_000450"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS5093141.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8FM_000451"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8FM_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS5093142.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8FM_000452"
     CDS             39330..40412
                     /locus_tag="ACG8FM_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS5093143.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8FM_000453"
     CDS             40412..41215
                     /locus_tag="ACG8FM_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5093144.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8FM_000454"
     CDS             41285..42025
                     /locus_tag="ACG8FM_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5093145.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8FM_000455"
     CDS             42036..43040
                     /locus_tag="ACG8FM_000455"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS5093146.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44927)
                     /locus_tag="ACG8FM_000456"
     CDS             complement(43085..>44927)
                     /locus_tag="ACG8FM_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093147.1"
                     /translation="SFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPA
                     DSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLI
                     KGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYA
                     GLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLI
                     LLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKW
                     YGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRF
                     YVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTD
                     KDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNY
                     VEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDL
                     AQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITE
                     ELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14448 a         7834 c         9347 g        13298 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagac
//
LOCUS       DAZUMT010000009        44862 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000009 DAZUMT010000000
VERSION     DAZUMT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.009"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1013)
                     /locus_tag="ACG8FM_000457"
     CDS             complement(3..1013)
                     /locus_tag="ACG8FM_000457"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5093148.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1193..1537)
                     /locus_tag="ACG8FM_000458"
     CDS             complement(1193..1537)
                     /locus_tag="ACG8FM_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5093149.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1530..1964)
                     /locus_tag="ACG8FM_000459"
     CDS             complement(1530..1964)
                     /locus_tag="ACG8FM_000459"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS5093150.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2102..2839
                     /locus_tag="ACG8FM_000460"
     CDS             2102..2839
                     /locus_tag="ACG8FM_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093151.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2832..4043
                     /locus_tag="ACG8FM_000461"
     CDS             2832..4043
                     /locus_tag="ACG8FM_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093152.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4247..5029
                     /locus_tag="ACG8FM_000462"
     CDS             4247..5029
                     /locus_tag="ACG8FM_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS5093153.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5099..5761
                     /gene="trmB"
                     /locus_tag="ACG8FM_000463"
     CDS             5099..5761
                     /gene="trmB"
                     /locus_tag="ACG8FM_000463"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS5093154.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5859..7613)
                     /gene="ade"
                     /locus_tag="ACG8FM_000464"
     CDS             complement(5859..7613)
                     /gene="ade"
                     /locus_tag="ACG8FM_000464"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS5093155.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7745..8674
                     /gene="comGA"
                     /locus_tag="ACG8FM_000465"
     CDS             7745..8674
                     /gene="comGA"
                     /locus_tag="ACG8FM_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS5093156.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8631..9680
                     /gene="comGB"
                     /locus_tag="ACG8FM_000466"
     CDS             8631..9680
                     /gene="comGB"
                     /locus_tag="ACG8FM_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS5093157.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9677..9955
                     /gene="comGC"
                     /locus_tag="ACG8FM_000467"
     CDS             9677..9955
                     /gene="comGC"
                     /locus_tag="ACG8FM_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS5093158.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9930..10400
                     /gene="comGD"
                     /locus_tag="ACG8FM_000468"
     CDS             9930..10400
                     /gene="comGD"
                     /locus_tag="ACG8FM_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS5093159.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10372..10719
                     /locus_tag="ACG8FM_000469"
     CDS             10372..10719
                     /locus_tag="ACG8FM_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093160.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10682..11107
                     /gene="comGF"
                     /locus_tag="ACG8FM_000470"
     CDS             10682..11107
                     /gene="comGF"
                     /locus_tag="ACG8FM_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS5093161.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11079..11438
                     /gene="comGG"
                     /locus_tag="ACG8FM_000471"
     CDS             11079..11438
                     /gene="comGG"
                     /locus_tag="ACG8FM_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS5093162.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11610..12617
                     /locus_tag="ACG8FM_000472"
     CDS             11610..12617
                     /locus_tag="ACG8FM_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5093163.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12642..13826
                     /locus_tag="ACG8FM_000473"
     CDS             12642..13826
                     /locus_tag="ACG8FM_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS5093164.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14263..15846)
                     /gene="abc-f"
                     /locus_tag="ACG8FM_000474"
     CDS             complement(14263..15846)
                     /gene="abc-f"
                     /locus_tag="ACG8FM_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS5093165.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15951..16079)
                     /locus_tag="ACG8FM_000475"
     CDS             complement(15951..16079)
                     /locus_tag="ACG8FM_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093166.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16424..16567)
                     /locus_tag="ACG8FM_000476"
     CDS             complement(16424..16567)
                     /locus_tag="ACG8FM_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS5093167.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16790..17269)
                     /locus_tag="ACG8FM_000477"
     CDS             complement(16790..17269)
                     /locus_tag="ACG8FM_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5093168.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17342..17626)
                     /locus_tag="ACG8FM_000478"
     CDS             complement(17342..17626)
                     /locus_tag="ACG8FM_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093169.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17704..17898)
                     /gene="ssrS"
                     /locus_tag="ACG8FM_000479"
     ncRNA           complement(17704..17898)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8FM_000479"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17970..19256)
                     /locus_tag="ACG8FM_000480"
     CDS             complement(17970..19256)
                     /locus_tag="ACG8FM_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS5093170.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19394..20065
                     /locus_tag="ACG8FM_000481"
     CDS             19394..20065
                     /locus_tag="ACG8FM_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS5093171.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20031..20519
                     /locus_tag="ACG8FM_000482"
     CDS             20031..20519
                     /locus_tag="ACG8FM_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS5093172.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20532..21479
                     /gene="prmA"
                     /locus_tag="ACG8FM_000483"
     CDS             20532..21479
                     /gene="prmA"
                     /locus_tag="ACG8FM_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS5093173.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21480..22244
                     /locus_tag="ACG8FM_000484"
     CDS             21480..22244
                     /locus_tag="ACG8FM_000484"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS5093174.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22477..24690
                     /locus_tag="ACG8FM_000485"
     CDS             22477..24690
                     /locus_tag="ACG8FM_000485"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS5093175.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24701..25147
                     /gene="dtd"
                     /locus_tag="ACG8FM_000486"
     CDS             24701..25147
                     /gene="dtd"
                     /locus_tag="ACG8FM_000486"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS5093176.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25318..26577
                     /locus_tag="ACG8FM_000487"
     CDS             25318..26577
                     /locus_tag="ACG8FM_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS5093177.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26666..27613)
                     /locus_tag="ACG8FM_000488"
     CDS             complement(26666..27613)
                     /locus_tag="ACG8FM_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5093178.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28007..29299
                     /gene="hisS"
                     /locus_tag="ACG8FM_000489"
     CDS             28007..29299
                     /gene="hisS"
                     /locus_tag="ACG8FM_000489"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS5093179.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29314..31083
                     /gene="aspS"
                     /locus_tag="ACG8FM_000490"
     CDS             29314..31083
                     /gene="aspS"
                     /locus_tag="ACG8FM_000490"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS5093180.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31192..32067
                     /locus_tag="ACG8FM_000491"
     CDS             31192..32067
                     /locus_tag="ACG8FM_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5093181.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32333..34216
                     /locus_tag="ACG8FM_000492"
     CDS             32333..34216
                     /locus_tag="ACG8FM_000492"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5093182.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34291..34575)
                     /locus_tag="ACG8FM_000493"
     CDS             complement(34291..34575)
                     /locus_tag="ACG8FM_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5093183.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34565..35299)
                     /locus_tag="ACG8FM_000494"
     CDS             complement(34565..35299)
                     /locus_tag="ACG8FM_000494"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS5093184.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35428..36051
                     /locus_tag="ACG8FM_000495"
     CDS             35428..36051
                     /locus_tag="ACG8FM_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS5093185.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36243..37364
                     /locus_tag="ACG8FM_000496"
     CDS             36243..37364
                     /locus_tag="ACG8FM_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5093186.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37371..40499
                     /locus_tag="ACG8FM_000497"
     CDS             37371..40499
                     /locus_tag="ACG8FM_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5093187.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40518..43712
                     /locus_tag="ACG8FM_000498"
     CDS             40518..43712
                     /locus_tag="ACG8FM_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS5093188.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43858..44565)
                     /locus_tag="ACG8FM_000499"
     CDS             complement(43858..44565)
                     /locus_tag="ACG8FM_000499"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5093189.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14394 a         7964 c         9409 g        13095 t
ORIGIN      
        1 ttttattttg atgaatcagt tgtttcttca gttgaagatt cttttgttga atcagttgat
       61 tttgtgtcag aactctttgt tgaagctgta gtttcacttg agtctttcgt acttgatgaa
      121 gttgttgtga atgcactcaa cacattttca aaggcatcat ctttgatttt tacatttgca
      181 tcttttaatt cttcaccgat tactttagtt gtgaatgcat tgtcacttag tttagtttca
      241 gtagcgatat cttttagttg atctttgtat ttatccatgt cattgccttt attttggttt
      301 ttcaccattt tgacaacgta gtattctgtc gcataagaag taggatttgt tgtagtaata
      361 acatcggaga tctcaccgtc tttcaatttg aaagctgctt cttttacttc tgcaggaata
      421 gttgttgtcg ttgagtcgaa tttaacttta ccgccatctt cttttgtctc tgtatcagtt
      481 gatttgtctt tggctaattt agagaaatca tctccatcat ccgctgattt tttcacatct
      541 tttgcttcat cttcacttga taatttgatg atttgtgctt ctacttctgg atggaaagat
      601 ttccaagcag ttttcaaatc gtcatctgta atatctacgt gagctttcaa gccagcttca
      661 aaagctaaat tgttacgaat atattcttta taagtatctt tcgtatatcc agcagcttct
      721 aattgtgatt caaaagtatc acctaatgat ttcgcttgtt tgtcgtattc tgcatccact
      781 tgcttatcgg ttactttatc gccatatttg ttattgaata ctttataaat gatcatccgt
      841 tgtactaagg attggttcga tgattcaagt tttgcttcat catagaaatc agatacagtg
      901 atcgttccgc ctttcatcgt tgcgatatct ttattagtat cgcctgaaca agctgctaga
      961 gttaaaacag ccaacgcact agtagctgcc aaaataattg attttttttt cattaaaatt
     1021 tgcactcctg taattagtta ttatttttat atattaacta taccatatcc catttccttt
     1081 aaaaacgttg tcgtggtagt ttcaaacaaa attcaaaatt tcatcacatt cctaggaaat
     1141 acgcaaactt ttctcactta tatagttaaa tctgttgaga aaataatcat tattatattt
     1201 gttcaggata ctctggcaga tcagctttta gtttttctac ttgttcattg atttgtgtga
     1261 tacgtggttc ggcttggaat ttaaaatctt cgatatcttt tttgattgct tcttggaaag
     1321 caggaatcac agattcagct gtttcttttg tttcgatcaa tgcgctttta aaatgattca
     1381 ggctgttatt taaatcattt gtcagagttg ttgcttcttc caattcttcg atcagtttct
     1441 ttcttgtttc attcccgctc ctgggcgcca ataacagacc tccgacagtg ccagcagcag
     1501 ctccaaataa cagtcctttg aaaaagtttt taatcatcgc aggacacctg ctttacgatt
     1561 gtttcttgaa tagcttttag ttcttccgat ccataactat cttgatgatt gacaaagtga
     1621 atagaaaaat catcctcttt gctgtagcga gggactaaat ggatatgtga atgaaaaaca
     1681 gattgatagg ctagttcacg gttgttattc aagatattaa gacctttcat ttctggaaaa
     1741 gctttttcca atgcttgagc aatcttaggc acacgcgaaa aaacctctcc tgcaagttcg
     1801 ggatcatatt caaaaatatc tgctacatga cgtttaggca caactaatgt gtgtcctttc
     1861 gttgcttgag agatgtctaa aaaagcatag accttgtcgt cttcatatat tttatagctt
     1921 gggatttctt gattgatgat tttgcaaaaa atacagtttt ccatataaaa catcctttct
     1981 ataaagaata aatacttcag cttaacttta ccataagtta aagctattgt aaggaaaaaa
     2041 acaaaatacc gtcattttct tcgtggtata ataaataaaa ataaatggcg gaggaaaaag
     2101 aatgagttta attatcgatc atgtcacagg ggggtacgga catctccctg tgttaaaaga
     2161 tatcagtttc gatgtacagc ctggagagat agtagggttg attggactga acggagcagg
     2221 gaaaagtacg accatcaagc acattatcgg tctgctggaa gcttcaaaag gaacgataga
     2281 aatcaatcat ctatccttaa agaaaaatac ggaagaatat cgcaaacaaa ttgggtatat
     2341 tccagagact ccttctttat atgaagaatt gacattgaga gaacatatcg aagtcactgc
     2401 gatggcctac gatataccta tggatatagc aatgaaaaga gcagaaaagt tgttggagac
     2461 ttttcggtta tctaatcgtc ttgactggtt cccagctaat ttttcaaagg gaatgaaaca
     2521 gaaagtgatg atcttatgcg cctttttgat cgagccttct ttatatatca tcgacgaacc
     2581 atttttaggc ttggatccac tagctatcaa tgcattgctt gaattgatgg tagaaatgaa
     2641 aaaagaagga gccggtattt tgatgtctac gcatatttta gctactgcag aaaaatattg
     2701 tgatcgtttt gtggtcttac ataacggaga gatacgagca gacggttctc ttgagatgtt
     2761 gagaaaagaa tttcagctgc cggattcttc tttggacgaa atctatattg ctttaacaaa
     2821 ggagcaggca catgagtagt ttttttcaaa aacgtttagc aagacatcaa aagaaaatga
     2881 tgaaatatat gcgttatgta ttgaacgatc attttgtttt agtttgtctt ttcttgattg
     2941 gcggtgtggg attatattat tctaattggt tgaaaaccct gcagccaccg tttgcaattg
     3001 gagggctcat aatctctgta ttttggatgc tctgtttatt cgtgggtaag atggctactt
     3061 ttgcagaggc agcagatatt gtttttttac ttcctaaaga aaaagaaatg caaacctatt
     3121 tggaacgagc ttttcgctat tcttgcattt ttccgttgat ctttttgata ttggcatgcg
     3181 gattggccat gccattagtg gtggttagta cggagcaatc cttttcgatg tttttcgtct
     3241 atctgatcat attatggagt ttgaaattca gtcatctgca aatcaagaga gcagaattgt
     3301 ttcgactaga tcaacaagtt attcgttatt ggaaattagg ctggtttttt tcttctctga
     3361 tctttttgtt tctatcattt tattggctaa tatgggcagg aatagcaggt gcattagtac
     3421 aggcaagtct atattactgg tttttgtgga agcaacaaga aaaaggattg gattgggaga
     3481 aaatggtgca tgtagaagaa cataggttgc atcagatcta tcaatttatc aatttattca
     3541 cagatgttcc tgaaattacg gcaaagatca aaagaagacg ctatttggat cctatattga
     3601 ctcatattaa aagagaatca aaaaacactt atctttactt gtttgcaaga agattggcaa
     3661 gaggttctga ttttagtggt ctttatttcc gattagtaat tataggtggg ttattgattg
     3721 ccggagtagt cgacttttat tttagtctga ttatcggttt cttattttta tatttgatcg
     3781 gtttccaact gttaccttta gcgaaccagt tccgctatat ggttctcgtt cagctttatc
     3841 caatccataa gaatcagaag aagcaggcga ttcagtatct actcacatgg gtattaatta
     3901 tcgcgtcggt tatttttggt ttgattgctg cagtcgtatt agatgggcag cagcgatggg
     3961 ttccgctagc ggcttatttg attgttacta tgctctttgt taaactatat gtaccaactc
     4021 gtttgaagaa gatgtcagac tgaaaacaaa gctgtgtagg gtaaaaatga gaatgaaaaa
     4081 tagagcgttg acagtaagaa tgctgttcgt tctatttttt ttgagaaaag ctggtttttt
     4141 gaatggcaat taaatgaaaa agaacgtata tgtgcttgac gtgtagtgtt caggcgacta
     4201 gaataataag tactgcaaaa caaagagtaa gaggacggga gaatgcatga tggagtttca
     4261 gttagaccaa gagtggcggt tacgtcccat caaaggcgac acaggaaaaa catacatagg
     4321 catgcgtgat gaggacaaag tctttattaa acgaaacacg actccgatgt tggctgctct
     4381 ttcaagggaa ggaattacac caaaacttgt ttggacaaaa cgaacgggga atggcgatac
     4441 tttgacagca caagagtggc tggaaggtcg cttgctgact cctaatgaga ttggtcaaag
     4501 aaacgacgtg attgatgttt tgtaccattt acatcattca aattctctta aaaccatgct
     4561 gacccgtatc ggtggtcgtg tcatgacgcc ggagaagatg ctgcaaaaat atgaaaaaga
     4621 attaccggaa gaactccaga aaaatcaatt tctgcaaatt gtccatcgtt ttcttcgtga
     4681 gcagatcccg tcaatagata aggaaaactt atcagtagta cacggagatg taaaccatcg
     4741 taattggtta gtatgtaaaa attatttata tctagtcgat tgggattcaa tcatgtttgc
     4801 agaccctgcg gtggatattg caacgatttt aggaaattat gtgccactgt ctagctggaa
     4861 tcagtggctg atcagttatg gcatacgccc gactgacgaa atgcttgaaa aatttcattg
     4921 gtatgcggcc atgaatttgc tccaagaaat caccagatat tgcaaacgtg aagatcatcg
     4981 acggatgaac gaagaaattt tacaattaaa acgaattttc agtggctgag gtttggccca
     5041 aactctgcgg agatttggcc agaccttttt aattagagga aaggaatgga gataaataat
     5101 gcgtgtccga aatcgtccgg gagcagcgga aatgttagcg gctcacccga attttgtaat
     5161 tagtgatcca acattgtgga agggaaaatg gaatgaattg tttgaaaacg atcatccaat
     5221 ccacattgag attggtatgg ggaaaggcca gttcatcact ggaatggcaa aagcccatcc
     5281 tgaaatcaat tatattggag tagaaatgca ggtcagtgtt gtttcgattg cactagacaa
     5341 gttgatcgaa caaccgcttc ctaacctgaa acttctacat gtggatggat cagcgttgac
     5401 agagtatttc gctgacagcg aagtagatca aatctatctg aatttttccg atccatggcc
     5461 aaaaaaacgt catgaaaaac ggcgattgac atataagact tttcttgctg tagatgaaca
     5521 gattcttcgt ccaaatggag aaatccattt caaaacagat aatcaaggat tgtttgagta
     5581 ttctctggca agtttttctc aatatggcat gatcctcaaa caggtatggt tagatttaca
     5641 tcaaagtcaa tttgaaggca atattatgac ggagtatgaa gaaaaatttt cttcaaaagg
     5701 tcaacgtatt tatcgagtag aagctcaatt tcaagataag accaaaaaga aaagtctgtg
     5761 aaactaactt tctgctttca taaaaacatt aatatccata aataaatccc agaaaatgaa
     5821 cattacagat gtaatgtatt ttctgggatt tatttgattt aaaatgtatt tatttcatcg
     5881 gtttttccac aaggtggaat caattggaag cgagaaaatg aaaataatcc tttgtcggtg
     5941 atcttcaatt cgggtatgac agataatgtt aagaaagaaa gtgtcaaaaa aggatcaaag
     6001 gttttagctg ctcctagttg atgtgcttta gttattaatt cattcaactg ttcatagacg
     6061 atcgaaaaag gttcctgtgt catgattccg cctataggaa gagaaacaca agctatttcc
     6121 tgctgattgt tgacaacggt catcccgcca cctttttgaa tcaaaagatt agcagcatac
     6181 agcatatcct cgtcgtttgt cccaacgaca actagattat gcgagtcatg ggcaatcgtt
     6241 gtcgctatcg ctccttgttt cagctgaaat cctttcacta tgcctaatcc aatgtttccc
     6301 gtttgatggt gacgctcgat caccgcaatc tttaattggt ctttttcgat tgaaggaatg
     6361 aatcgttcgc cttgtcggtc cactttttct accaaatctt tagtcactag actattcggg
     6421 ataatttcga tcacatgggc ttgtgaagaa gaaagaagca gctctaacga atgtggattt
     6481 aatggttgag cgtttagttt tggcagacaa cacgtatatg aatttgaatg atcagcaaat
     6541 aaatggtctt ggagttgccc agattgaaca actaatgttc ccttatagaa aacttcatga
     6601 attggcagct tggtaagatc gtctgttaag aagaaatcag cttgataccc aggggcaatc
     6661 gcacctagtg ttttcaaatc aaaacattct gctgcattca atgttgccat ctgaatggct
     6721 tgtaatggat cgattccaag tgcaatagct tgacggacga tatgatcgat tgatccttct
     6781 tttactaaat cattgatcag tttatcatcg gtgacaaaga agcatcttct tgaattttca
     6841 ggtgtgatcg ccggtagtag cgatgccaga tcttttgcaa cggtcccttc tcgaaccatt
     6901 aaatacatac caagatccaa acgttcttta gcttcttttg cagttgtagc ttcatgatcc
     6961 gtccgaatac ctgctgcagg ataaacattt aactcttcca tgccgatacc agctgcatgc
     7021 ccgtcaattt ttttcttatg ctggtgcatc aattggatct tttctaggat gtctgtttca
     7081 ttatttgcta ctgcttgata gttcatgact tcagctaagc ctaaaacttc gggtttttgt
     7141 aaaaaagggg ctaatttttt tgcatttaaa tttgcaccat tggtctcaaa aggggttaca
     7201 ggtacacaag aaggtagcat aaaaaagata ttcatgagtg tttgtcttgc atcttctatc
     7261 ataaactcaa gacctgtact cccagcaatg tttcctattt catggggatc tgtcactaca
     7321 gtagtaactc catgaagcaa ggatactttt gaaaattcgc ttggtgataa aagtgagctt
     7381 tcgatatgca catggccatc gatgaatcct ggcactagat attgcccatt agcattatgt
     7441 acgctttttc cttcgtattc accgattcct acgatcattc cttcgcagat tgctacatct
     7501 ttttccatga tttctttcgt aaagacgttg acgatcttag catgcttgat cactaggtca
     7561 gcaggctttt ttttacttgc tacttcgata tatttcctta atgttgtttc caatggagtg
     7621 cctcccaaga taaattttta taagtataac ataaattcgg tttttgccaa tatttttttg
     7681 taaattcatt ttatttgaaa caaaaagatc aaaaagacgt atgttttttg tggaggggag
     7741 aataatggaa ataaaacgat tatcggatca aatgataagt gaaagttttg ccaaacatgc
     7801 ccaagacgta tatatattgc cagcttcaga agtattttac atctattttc gctatggaaa
     7861 aaaacgaaca ttctggaaga aagtctcttc ggcagatgga cagcaactga tctcaagatt
     7921 taagtatttg ggacagatgg atatcggaga aaaaagaaaa gctcagttag gtgcatttac
     7981 ttattcttta gaaaaacagg aagttcgtct gcggttgtct tctgtaggag attaccttca
     8041 acgagagagt ttagtgattc ggctattata cggtactttt gaaacacccc atcgttgctt
     8101 tagttctcga gattttgcaa ctattttaca gaaaaccgtc agacgtggat tatacctttt
     8161 cagcggacca gtcggatcag gcaagacttc tctaatgtat cggttggctt tggaagaaaa
     8221 attgcaagtg attactatcg aagatcctgt agagatagaa gaaccgtttt ttctgcagct
     8281 acagatcaat ccgaaaatag cacagactta tgatacactt ttgagactcg cattacggca
     8341 tcgtccagac ttgctaatca ttggcgagat aagagatgaa tcaactgcac atgctgcggt
     8401 aagagcagca ctaacagggc accgggtttt tgcaaccgta catgcacgca atcttgaagg
     8461 tacattggtc agaatccatg aactgacaaa gaaaaaagaa gagctatctg aatgtttagc
     8521 tggtgtcatt tatcaagaac ttttggaaga tgccaatcac catccttctg ttttatggag
     8581 ttatcaattt atggaggaag aatcagtaaa aaaatcatgg gaggaaagtt atgaagaagc
     8641 tcaggaaaat atttggcagt cagaactatt ctaaaggatt aacaagaagc cagcaaaacc
     8701 agtttgcaca aaccatggga gaactgcttt tatctggttt tagtctgcaa gaatcccttt
     8761 atttattact cacgatccgt tcgtttccac ctgttatttt ggcaaatatc caacatcagc
     8821 taaaggaagg gcaagcgttt agtgaggtac tttcgcaaat aggatatgga gcagagcaac
     8881 tggtacagat cgagttagct gaacgtcatg ggaatctgcc gacaactttg ttggaaattg
     8941 ccaaacagtt tcggcttgtt gaaggatttc gcaatgattt gaaaaagctg cttgcatatc
     9001 cgatgctatt gctcaccttt ttgctaggta tcttgtttag tatgcggttg ttcgttttgc
     9061 ctcagcttat tggttcagga atggtcatgc aagagcattg gggtattcag ctgatacaag
     9121 tctttcattg gtatgttcta ctattcatcc tagcggttac tctattggct ttactagtaa
     9181 ggtatcgtct gatgaagttg tctcctgttg atcagagtga atggataagc agacaattat
     9241 tgattggacc gatatattcc ttgtatcaat cggcttattt gtcactagag attggaaagc
     9301 tttttcatga aggtttcgaa ttaaggcaaa tcatagtctg cttaaaggaa acgaaacaag
     9361 aaagtctgat tcagcatctt gcctctaagt tgattcaagg attggaggca ggtatgtcat
     9421 tggctgagca gttccagaat tatcgttttc tatcagaaga attcagtaaa attgttttac
     9481 agggagaggc aaagggaagt ttaggtaaag aactgatttt ttacagcgaa tggacaagaa
     9541 aacagctttt caaacgtctt catcactgga tgcattttat tcagccaatc atttttttgg
     9601 gcgtagcggc tttgatttta ttagtttatg ccgctgtttt attacctgtt tacggaagca
     9661 tagaggaggt tttgccatga aaaagagaaa aatatcttat tatagcggtt ttacattgat
     9721 cgaaatgcta atcgtcttac tgatcatcag tgtattagtg ttgttgttcg tccctaattt
     9781 gtcgcgttat aggaatcatg tagaccaaga aagtcgtgaa gcgatcatcc agttggtaga
     9841 cacacaaaaa gagctctatg cgcttcaaaa taatggaaga gttcctactg tggaggaatt
     9901 gttgaatgaa ggctatatca aacgagaaca tgcagaaatc tatcagcggc cttgaaggta
     9961 aggttttagc atcaggatat acgttgtttg aatcactctt actgatttta ttacttacaa
    10021 cgatcctttc ttttccagtt ctcgcatttt ctgcatggaa gaatgaattg gcagtctatc
    10081 aatttttcgg ccaatttgaa aaacgaatct attctacgca aaaaatcgct attgtgaatc
    10141 agatccaaac aggcttttat tggaatagtg aagagaatca aatcattttt catgttccga
    10201 atccagataa aataaattgg gaagtattag atattcctga tgagattacc ttgaaacgcc
    10261 atgcttctat tacttttgct gctagaaccg ggaatgaaag ttcactaaaa gcttatcaat
    10321 tttattggga agaaaaagag cggacgatta cgtatcaatt ccagataggg agtggtcgct
    10381 atacaaaaag aattgaataa aacaggattt gttttgttgg agtcccttac agcgcttatg
    10441 atcgcttctg tctgtgtcta cttagtgagc tttggccagc tgtttttgct tcaggcagaa
    10501 aagaaaagtg gggaggaact gctatttatt cgaatgattt atgaagatat caagacgcag
    10561 cggatgtatc acaaaagacc agagcgaagg gaagaaatga tcccatacga tatccaaatt
    10621 gtcgataata atggagtaat cgaaaaagtg tttgtcaata tggagaaaga agcgatcagt
    10681 attgaaaaaa caagtaattc cagcatttac gctgattgaa tgtctaattg gactgatgat
    10741 attgagcagt ctattgctca cattcaatct tttgattcag caaaccatcc aagtaagtga
    10801 atttttgcag cgtaaagatc aaaaagagtg gctaatattt ttagcacaat tagaagaaga
    10861 actcaagagc agtcacaatg tctctgtgaa aaatcagcaa ctgcactaca cgatcgagga
    10921 taaacagtat atcatcgaaa gatatcaggc aatgatacgt aaaagaagaa cttccggcgg
    10981 acatcaacct atgttaacaa gtatcactga gcttcaatta ttggaaaaag aacacacaat
    11041 ttctttttat gtacactttg aaaatggaga agatggatat gccatatgga caaaaaacga
    11101 tcagtagaaa atgttataag ggctctattt tattttctgt cttgctttta tttcttttat
    11161 gtacgttttt attgatggca gcattagaaa gctaccgttt gtcaatggat cttaatatac
    11221 gaatcaaaaa ttattatata gcaaaaataa tgaagcagat ggctttatcg gaacagtcta
    11281 cgcttgccga aagttcggaa ggagtttttt actacacaac tggatctgta acatatcagt
    11341 gggtacaaca gtccctattc ttagaggtgg aagtctcacc gtttatcttt cggttcacag
    11401 aagaagtaaa aaatgataca gatactagta cagaatgatg gaatgttcgt gaaaacgccc
    11461 tcttttctgt tataacactg tttttaataa aaaaatgttt gccgtttcac aaataaaaaa
    11521 cagtgaaaac gattgaaatt ttgtcaattg ttcgatagaa ttaaacaggt gaaaaaattg
    11581 ttataatata tctagaaatg aggtgcacca tgttaccaga aaaaatggaa caagggttta
    11641 atcaaaacct ggaagctatt cagcttttac agaatgcatt gggaacttcc tttttagaag
    11701 cctatgtcga aaatgcagaa aatttaatag atgattatca agtacgtgtg gtggatggag
    11761 tgccgacaaa agagacaacg caacggatca cagctcttta tgaagagttg aagaaacttt
    11821 ctttcgagcc agaagaatgg cgcagactgt ctcaattact tttattgaag ggcagccaga
    11881 cagaacattt gcaggcgaac catcaactca ctcctgatag tatcggtttt ttatttgttt
    11941 ttcttattga acaactatac acaaataaaa aagcaccggt taagattctg gatattgcgg
    12001 caggaatggg caacttgtta ttaacagttc ttttaaacct atcgaatgcg gggtaccaaa
    12061 cagaaggaat tggtgtggat attgatgata cattgttggc tgtcgcagct tctacaagtg
    12121 atttgacaca agcaaatgtt caatactttc atcaagacgg gttgcaggaa ttattaatag
    12181 atcctgtcga ttttgcaatc agcgatttgc caatcggtta ttatccgaat gacgaaaaag
    12241 caaaagagtt tttaacaagt accgaagaag gccatagtta tgcacatcat ttattgctag
    12301 agcaatcaat gaaatatgtg aagccggatg gttttggttt gtttttgatg ccgagtggat
    12361 ttttagaaac ggatcaaagt gaagaaatca aaaaatggtt taaagaagaa ggttatcttc
    12421 aaggaatgat tcaattgcca gatgaattgt tccgtaataa acaatcgcaa aaaagtattt
    12481 tgatcttaca gaaaaaagga ccacaagcac aacaagtaaa agaagtcttg cttgtgaaac
    12541 ttgcttcatt aaaagaacca gagaaggtga cggaattttt caacgagttt aaaaactgga
    12601 aatcatctgc tctgtaatca ttcaacgaga aggagagtca tatgtctaaa acaattgcaa
    12661 tcaatgctgg gagttcaagt ttgaaatggc aattatatca aatgccagaa gaaaccgtag
    12721 ttgcgaaagg gatcgtcgaa cgtatcggtt tacaagattc tatttttacc atcaagtacg
    12781 gcgataatca aaaattcgaa caaatcatgg atatcgaaaa ccacgaagta gcagtaaaaa
    12841 tgctgttaga tcagttgatt gaactgcaaa ttttaagctc ttatgatgaa atcaccggag
    12901 tagggcaccg tgttgttcac ggaggagaaa cttatgggga ttcagtagtg atcgatgaga
    12961 aagtaatgga ccgcattgct gaattagcag aatttgctcc tttgcataat cctgcaaact
    13021 taatggggat caaagcgttt aaaaagattt tacctgacat tttgagtgtg gctgtattcg
    13081 atacatcttt ccactcaaca atgccaaagc aaaattattt atatagctta ccggtgtctt
    13141 attatgaaga tttcaaagta agaaaatatg gcttccatgg cacaagtcat cgttatgtgt
    13201 cggaacgtgc ggcaaaatta ttgaacaaac cgatcgaaga actaaaaatc atcacttgcc
    13261 acttaggaaa tggtgtatcg atcacagcag tcgatggagg taaatccatg gatacttcta
    13321 tgggattcac accattagct ggggtgacaa tgggtacacg ttcaggtgat atcgatccag
    13381 ctgttctccc atatttaatg gaaaaattag agattgatat tgatgaaatg atcaatgttt
    13441 tgaacaagaa atctggtctg ctaggcctaa caggattgtc tagtgatatg cgtgatcttg
    13501 aaaagaacta tgaaaaagaa catattcgtt tagcttatga cattttcgtg gaccgcatcc
    13561 gaaaatacat cggcggttat gtgacaacga tgaatggtgt tgatgcaatt gtctttactg
    13621 ctggtatcgg cgagaatgac ggacatgtac gtagtgaaat catcaaaggt atgtcttggt
    13681 ttggttgtga aatcgatcca gcgttgaatg aattgcgtgg tgaagaagta gatatctcta
    13741 caaaagaatc aaaagtaaga gtattagtga tcccaacaga tgaggaacta atgattgccc
    13801 gtgatgtgga gcgtctgcgc ggctaagcca aagcattcgt taggataaaa gatgaaacgc
    13861 atgcaagagg gaattcttgc atgtgttttt tttgcggaga agaccgcaaa atggtaattt
    13921 aacttcaatt tcccttcttt tttacaggat cttcttaggg aattttgctt ggaaaagtgg
    13981 tcaaaaggga aaagactggc attttattta ttgattttca ggaaaatccc aatattttat
    14041 tttcattcat ctttataagg gacattacat atgtaaaaag aaaaaacatt tttagatcag
    14101 cctcgtgggc tgtcgacttt attcttcgct ttatgtttag ctagaaaacc gatccgaaac
    14161 attcattgat cgaataataa aaacttgatt ttccacctgt actttgcctg aatcgtgaaa
    14221 gcacaggtat gaaaatcaag ttttttgttg cgaaaaaacg tcctagtcaa tcagttggcc
    14281 gagcggctgg cggctgatct gctcctgatc aataacgagt gcttcttgga agtgttgctg
    14341 caaaaagtag cggtcatggc tgactgctag gagcgttcct tgatagtctt gcagcatttc
    14401 ttcaatttct tctcgagcat agctgtcgag gtgattggtt ggttcatcca aaatcaggac
    14461 gttgattttt tctaaaaaga gtttcagcaa cgccaaacgg attttttcac caccggataa
    14521 atctttgatc cgtttaccca catcgctttg ataaaagcca cttttggcta attgccgtcg
    14581 agcgacttct tcgtttccag tcaatgtgat ggcgtaagcc agcaaacggc tatcgggatc
    14641 ggcaaactgc agctgctgcg gcaaataacc gatttgcaag ctactaccta atttgatggt
    14701 gccaccgtaa ggcttttcct caccgagtaa taatttgagt aaggttgatt ttcctgcacc
    14761 attgtcgccg atgatggcta aatgatctcc acgatagact gcaaaactgc tgtctttgaa
    14821 tagtacttga tcagcgtact gctggtgcaa gttttgaatg atgaaaactt ctttgccaga
    14881 ttttccacca ttagacaagc tttgcagccg atttttcggg ggaggtggcg gtttgacgag
    14941 tgtcagtttc gccaggcggc gttccagttc tttggctttt ttgaagaagg actcattgtc
    15001 tccttcatgt gcccactgac gaaaacgacg aatcatgact ttcaaccgct ggacttcttt
    15061 ttgctgcagt tcgtaatttt ttgtcagctc agccaagcga gcttctttca agactcgaaa
    15121 acggctgtag tttccgggat actcaatcaa tgcaccttct tccagttccc atgtttttgt
    15181 ggtgacttga tcgagaaatt gccgatcatg agaaacgacc agaaccgatt tttttgtatt
    15241 ctgtaaatag ttttccaacc agcgaatact gttaagatct aaatgattgg tcggttcatc
    15301 gagtaaaagt aaatcggcat cggctgctaa aattttggct aaagcgactt taactcgttc
    15361 gcctccgcta agttctgtta accgtgcttc ctgacggtgt ggaatcccta agccttgcaa
    15421 gatcgctgca atccggtctt ctaatacgta accgcctagt tcttcaaagc gatgctgcag
    15481 ctcaccgtaa cgggccagac acttctctaa gtttgtatct ggttctgtca tcaatgcttc
    15541 gtagcgactc atttgctgtt tgaggtcctg tatctcagcg gcagaatgga gcaagtagtg
    15601 gttgacggtc gcttcggaaa ctgtaagttc ttgttcgacc gtcccgatct ttaagccttt
    15661 tttgcgactg ataacaccgc tgtcgacacc ttctctgccg gtcaagattt gtaaaaaggt
    15721 tgatttgcca gttccgttga taccgactaa ggcaattttt tcaccagctt caatttgaca
    15781 ggataaatcc tcaaataatg gattcccgcc gattgttttt ttaatagaaa ttgcttgtat
    15841 gatcatgatc tctctccttg agaccactca aaaaggccat ggttatccat ggcctgtgtt
    15901 atttaaactc agattatcat gagaaaaata ccttgagatg gccattgatt ttacgtgaat
    15961 tcaactgttt tggacaaact tgtcccgtca acagcaactg taaaaccaat tgcatggcta
    16021 aatgcgatat ggtatcgttg gtaattttca ttacttgtta ttttagtcat gcgcatcata
    16081 gtattttccc tccttcgtgt agttgtcttt tccagtgtac ctgaaagtgt cagaaagttc
    16141 aactagcgct gacatttact taatatttat cgatgtgtaa ttctgtcaga gagctttttt
    16201 tatctgactg agggtaatgt ctcgtggacc acatcattgt attctgagct aaaccagcgt
    16261 tcaggcgaaa tcatatggtg tttgataaaa aactttgtct acatgacttc cttttgccat
    16321 ttgcttaata ccgttgcatt aaacagctct tctctttgga aatctcacca acatcgcgtt
    16381 caggagtatc gttgtggttg caatggcaag cggtagcctt tcatcaattc ttgaatgatt
    16441 taccgaatgt agcacttgcc ttctcgcggt acccgaaccc gaaataaaat tttagtttgg
    16501 cgataaaatc ggtatccgcc atcgatactt catatttttt cgtatcagat tcatcggaaa
    16561 cttccacatt gacgaaccaa taagctttgg catgcttagg tcgttttaaa ccacaccagg
    16621 gcgactaatc gccctggtgt tcactttttt ttattttact tgaactcatt gactgttttc
    16681 cctttatttt aaacattata aagaccgatg agtgcttgcc gcgtcttttg tttttatcac
    16741 ggttttttgt gcaaaaaaac ctgccgatag ttggcaggtc gtctacgtgt taagctttgg
    16801 tgtggtcttt agctggcaat tggttttcta aatcagttcg gacaacaagc acatcgcaag
    16861 ctgcgtttct aataacatat tcagaaacag aaccaataaa taaacgttct acagcattca
    16921 atcctgtggc tcctaacata atcaaatcta cttgattatc ttctgggatt tctctggcaa
    16981 taatttgttt tggtgagcca tattctacga cagaggttac attattcaag ccagctttct
    17041 tcgcattgct ttcataatct gccaatgttt gctttgccat ttcagtagct tgttctgcaa
    17101 gcattccatc aaaggatgaa acggtttgga aagaacgtgt gtcaatgaca tgtgctaata
    17161 gaagttctcc gttatttcgg atagctacat tgaccgcctt tttaaaagct aattcagctt
    17221 catcagaacc atctacggca accatgattt ttttgtattg ttgtaacata agcattcact
    17281 cctcaagtca atcgtattgt actcttttat tgtagaacac tttagggaaa aagagaatta
    17341 tttactgctt tggttcagca attttttttg caaatcggat actgaaggtg gcagaaacaa
    17401 taattacgat cagtaaaaag accaaggcaa taaagcgttg attgaaaaaa gctgttaaaa
    17461 tacccattac gcctaaaaca gcatatgcag ctccgtaaaa ttgtaggaat ccttgatttt
    17521 tatcattttt gttgatcaat acaaaaaaga tatcctgttt attaaataaa taatagctaa
    17581 cgaataataa tactaaagtg gcgataatca ttaaaatcct aatcatgaac aaatcctcca
    17641 aaataagcaa caaatagaaa aagcaatttg ccaagagcaa attgctttat tctttaaaag
    17701 atggtaatct ccgttaatgc cgttgtttgt cctgtagtat tgatcccgcg aattcaagca
    17761 ggtgggtccc atggtcgtag cttcgaacta ccaaatgata aggcatgtta ccagcagtga
    17821 cacaagacgg gtcccaagat atcaataaaa atgttcggtc aacacacaaa gaggacaatt
    17881 cgaacatcac agatttccca cctctatagt agcatatgaa tttaaaaaga gcaatgagag
    17941 agctcgcaga aacagaaccg ttttcttgtc tagttttttg gaggatgctt tttccactct
    18001 tttatacggt aatactgttg acccagagcc tgttcatatt tccctgtatc ttttggctga
    18061 taatactgag cattcttcag tttatctggt aaatattgct gattgaccca agcctgatca
    18121 aaatgatgag gatactgata acccacgcca cggttgagtg atttcgcacc tttgtaatgg
    18181 ctatctctca aatgatcagg aacatctcca gctttgccct ctcgaatatc agcaagtgct
    18241 gcatctaatg ccatgtaggc agaattggat tttggcgaca agcaaagatc gacgacgacg
    18301 tctgctagtg ggatacgtgc ctcaggcaat cccaattttt cagcagctaa tacagcgttc
    18361 actgtacgag ccgcagcagc aggattacct aaacctatat cttcataacc gataaccatc
    18421 agacgccggc aaatacttgc cagatcacct gcctcaacta aacgggcgag ataatgaagt
    18481 gcagcatcca catcacttcc acgaatcgat ttttgaaaag ccgaaataac gtcataatga
    18541 gcatctccat ttttgtcatg ggtaagagct tttctttgga cacattcttc aatgatcgac
    18601 agtgtaagat gaatccttcc ttctttatct gaaggagtcg atagtgtagc cagttccagc
    18661 ccattcaatg cactccgcaa atcgccgttc gttgcacgcg aaaggtgaag aagagcatct
    18721 tcgtctaact ggattgcttg ttgtccaagg ccgcgttctt tatcccttaa agcatgttcg
    18781 actgccaact ggatgtcttg ttctgttaat ggtttgactt caaagatctg tgtacggctg
    18841 cggatcgctg gattgatggt aatataagga ttctcagtag tcgctccgat caagatgatt
    18901 cggccacttt ctaaatgagg aagcagaaaa tcctgttttg ttttatctaa tcggtgtact
    18961 tcatccaata agaggatcac tgtaccactc atttttgctt cttccgctac aacttgtaga
    19021 tccttttttg aatctgtagc ggcgtttagc atgcgaaatg cataattagt agagccggcg
    19081 atcgcacttg cgatactcgt ttttccagta ccaggaggtc catatagaat catggaagag
    19141 agcatgcgag catcgaccat gcggcgtatg atttttcctt ccccgactag atgctgctga
    19201 ccaacgactt catcgatcgt tcgaggtctc atgcgataag ccaatggctg ttgcatagga
    19261 ttttcctcct tcttgttttt acgtccctta attatatcat atttagcaag atgcctagaa
    19321 agaacagcct tctgttgttt tgttttcata gcttatccaa ctaatattgt gtatgataaa
    19381 gtaggtgaga aaaatgacgg ataaacaaaa gatagatgaa tttttttgga ataaaacgac
    19441 gccagaagtg gcacgggatt tattggggat gtatttggag catgacacac cagaaggaag
    19501 attagctgga tatattgtag acgcggaagc atacctaggt ccagaagacg aggccgccca
    19561 tagctatggt ttaagaagaa caccgcgtgt gcgggcaatg tatgaaaaac caggtacgat
    19621 ctacctttac acgatgcata ctcatcggat tttgaacatc attactcagc cagaagggat
    19681 tcctcaagga gtaatgatac gtgcaatcga acccgccgca atgattgatc aaatgagtaa
    19741 aaatcgtggc ggaaaaactg gtccagatat cagcaacggt ccgggaaaat tagtggaagc
    19801 attagcaatt cctcaagaac tttatggtca gtctatagca gatagttcgt tacgcttagt
    19861 ttttgagaaa aaaaagacac caaaaaagat caatgctttg cctagaatcg gtatcccaaa
    19921 caaaggtgta tggacagaaa agccgttgcg ttttgttgtg tcgggaaatc cttatatctc
    19981 tttacaaaga aaaaaccaaa tcgagaaaga ttggggttgg aggaaagaaa atgaaaaaga
    20041 aggatctatt aacatattta gatgaaataa ttgaaaaaca agtgacagac tatgatgtag
    20101 ctttagattg ggatacaaaa aatcatacga tcgagatcgt tgttcgcttg tttgcagaaa
    20161 ataaagcaca atttgagatt gatgatgcag aagggatcgt ttcgcaagaa ccaattatcg
    20221 aatttgaaga cggcgtactt ctttttaatc ctcagaaatc tgtttttgat gaacgcaatt
    20281 atctagcagt aatcccttat gaaggaaaaa aagggttggc aaaatcagta gctgattctt
    20341 tagtggaata tctaaatgaa gtattagctc aagggcaaag cgatttgctt gattttttgg
    20401 atgaagacga tgacgaagct gtattcgagc ttcactggga taatcagcaa ctagcagaat
    20461 tagtagaagt aaaacagcaa aatgaagcag atacttattt accgtaccca agttattaag
    20521 gagtgacaga tatgaaatgg aatgaagtaa aagtagaaac tgaaagtgaa gcagtcgaag
    20581 cagttgctaa tattttgatg gaggcagggg cgagtggtgt cgcgattgaa gattctttag
    20641 atgttgaaca ttttcagtcg gatcaatatg gtgagatttt agataaaaat gattttacat
    20701 cgctgaaaga aggcgcacta gtcatggctt attttccgga aacgactttc ttaccggaaa
    20761 tcttgccttt tatcaaagat cgtatcacac aattacctga gtttggtcta gctattggaa
    20821 aaaataaagt aacagttagt gaagtagagg aaagcaactg ggcaacagcc tggaagaaat
    20881 actatcatcc ggtgcgtatt acgcgaatgc tgacggttgc gccaagctgg gaaaactacc
    20941 agacagaaga tcctctagaa aaaattatca cactagatcc tggtatggca tttggcacag
    21001 gcactcatcc cacgacttcc ttgacattga cagcactaga aacagtcttg cgtggtggtg
    21061 aaacattgct agatgtggga actgggtcgg gcgtgctaag tattgcagca aaatatcttg
    21121 gtgcaaaaga tgtctatgcc tttgatttag acgaagtagc cgttcgttcg gcgaaagaaa
    21181 atatggatat gaatgaaata gccaaagatg tccatgtatc agctaacgat cttttgacag
    21241 gaatcgagat tgaaagtgat gtaatcgttg cgaacatttt agcagatatc atcttattga
    21301 tgatcccaga tgcttggcgt ttactgaagc aaacaggcac gctgatcgtt tcaggaatca
    21361 ttgaagagaa gaagcaaatg gttctggatg ccatgttaga acaagggttt atcgtggatc
    21421 aaattttcca gcaaaaagac tggtatgcaa ttattttgaa aaaaccagag gtggaataga
    21481 tgcagcggta ttttttaaaa gaatcatatg aatcaaaaga ctattttgtt ttagaagggg
    21541 aaaactatca ccacattgta cgggttatga ggatggaacc cgaggatgaa gtttttttgg
    21601 cattcaatga tcaaatagcc attgttgcag aaatcacgga gattactgaa gaggctgttt
    21661 atctgaaaga aattaaaaaa gaagaaaatc aaaaggaact gccaagttcg attaccatag
    21721 cgagtggcta tcctaaaggg gaaaagctgg attggatcgt tcagaaatcc actgaattag
    21781 gagcgcaagc ttttcttggt ttcccggcat cagcctctgt cgttaaatgg gatgcaaaaa
    21841 aacgtatgaa acgacaacaa cggcttgaaa agattgctca agaggcagcg gaacaatccc
    21901 atcggcagat cgtgccatca gtagagttat tggaaacgaa acaagattta tttgagcggc
    21961 tagatgatta tgacgtggtc ttggttgctt atgaggaatc agcaaagcaa ggcgaaaaat
    22021 caaatgcagc caaaattctt agtgaaacaa aaccagatag tcgaatctta gctgtctttg
    22081 gaccagaagg tggattatcc cgcgatgaag tcgattattt tgtagaacac aaagggatac
    22141 tttgtggatt aggaccaaga atccttcgta cagaaacagc acctctttat ttattaagtg
    22201 ctgctagcta tcaatgggaa ttaatgaatc cttctttcag ctaacgttct tcattaataa
    22261 aagagtatcg acagaaagaa ttgaaaaaat agtcaacaat ttgtataata gaaacaatta
    22321 tttatacaat ggatacgaaa aatcgtttga aaaagctttt aaaaagcgaa catttccgta
    22381 aaaaaacgta taactgcacg gattttggga caattatcaa acagatattt gtcccgattc
    22441 ccgttttacc gaaaaaagaa agggggatcg attcctgtga caaaagaaga aattttaacg
    22501 ggacccggtg tcataaagct cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt
    22561 gagaaagcat gcgaatacgc gactgctgca catgacggac agtttagaaa atcaggcgaa
    22621 ccctacatta ttcatcctat ccaagtcgca ggtatattag cagatttaaa aatggatccc
    22681 catacagtgg ctacaggctt cttacatgat gttgttgaag atacagaaat cactttagaa
    22741 gatctgagag aagaatttgg cgatgacgtt gctatgttag tggacggcgt aaccaaatta
    22801 gggaaaataa aatataaatc ccacgaagag cagctagcag aaaaccaccg aaagatgctg
    22861 ctagcaatgg ctcaagattt acgagttatc atggtcaaat tagctgatag actacataac
    22921 atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa ggatcgctca ggaaacttta
    22981 gaaatctatg cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg ggaattagaa
    23041 gatacagcac ttcgttatct aaatccaaaa cagtattacc gtatcgtcca cttgatgcag
    23101 acgaagagag aagaacgtga aaaatacgta agtggtactg ttgaagatat tcgaatagcg
    23161 acagaagagc tggggatttt tgcagaaatc tatggacggc caaaacacat ttattcaatc
    23221 tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt gctagctatt
    23281 cgagtgatcg tagattcaat caaggattgt tatgctgtat taggagcaat ccacacaaaa
    23341 tggaaaccaa tgcccggcag atttaaggat tatattgcca tgccaaaagc taatatgtac
    23401 caatctttgc atactaccgt gatcggtccg gcaggaaatc cggtagaaat tcaaatccga
    23461 acacaggaaa tgcatgaaat cgctgaattc ggggttgctg cacactgggc ctataaagaa
    23521 ggaaaaaatg aaaaagtaga accagatggt atgacgaaac aattaagctg gttccatgag
    23581 atactcgaac ttcaagacga aagctatgat gcttctgaat ttatggaagg cgtaaaagga
    23641 gatatcttta gtgataaagt ctacgtcttc acaccaaaag gagacgttac tgagttacca
    23701 aaaggatccg gaccattgga ctttgcatac agtatccata cagatatcgg taacaaaacc
    23761 actggtgcaa aagtaaatgg caaaatggtg cagcttgatt acaaattgaa aaacggagat
    23821 atcattgaga tcatgacttc tccaaattca tttggcccaa gtcgcgactg gttgaaatta
    23881 gttgctacta gcaaagcaag aaataagatc aaacgtttct tcaaagccca agatcgagaa
    23941 gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct aggatttacg
    24001 cctaaagata ttttgacgaa gaacaaactg caagaagcac tcgatcgttt taattatcaa
    24061 acagaagatg atctctatgc agctgtaggg tatggagaag ttagcccctt gacgatggcc
    24121 aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca
    24181 gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt
    24241 ggcgttgtca ttcaaggggt agaaaactta ctaattcgta tcagtcgctg ctgtaatccg
    24301 attcctggtg atgatatcgt tggttatatc actaaaggca gagggatatc cattcatcgt
    24361 cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc
    24421 gaatgggaag atacatcgaa tacacgaaaa gagtatgatg cagatttgga aatttacggc
    24481 tataatcgtt caggcttatt gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat
    24541 ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg cgacgatcca tttgacggtt
    24601 ggtatccaga atttatccca cctaaagagt atcgtggata aaatcaaagc agtacctgat
    24661 gtctacagtg tacgccggac gaatggatag gaggacagat atgagagcag ttgttcaacg
    24721 ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg gctacggcct
    24781 acttgtttta ttaggcattc atgaaaccga tacgcaaaaa gatgtggact atctggtaaa
    24841 aaaaattgca caaatgcgta tttttgaaga tgaacaagga aaaatgaata ttagtgtaga
    24901 agatgtggga ggacaaattt taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa
    24961 aggaaatcga ccaagctttg tggaagccgc acgaccagaa acagctattc ctttatacga
    25021 atcattcaat gaaggaatca gaagtcgcgg catcacagtc gctaccggac aattcggagc
    25081 agatatgtcg gtttctctcg tcaatgatgg acctgtaacg atcattattg attctaaaaa
    25141 caaataaagt aatctggttt tcactagtaa aaactatgaa acacgtcgct taagttagac
    25201 tgataaactc taacttaagt ggcttttttg tctgcaaaaa gcgcttcttt tgtcagcagt
    25261 cggaaaaaaa gttaagctta aattatacaa gttatttttt ataggaggtt gctgaagatg
    25321 aaagaagtac cagtagtaga tatcaaaatc acagatttaa aaaaattgta tcaaaaagga
    25381 tacaacatgt tggaggagtt acgccatgaa gcggacgctc ccgtcgtcaa agcaaagatt
    25441 ttcaataaag aagctatcac gatctacgga tcatcagcag caaaagtatt ttatgatcca
    25501 aggaatttca aacgaaaagg tgctatgcca aaattagtat tgaagacact ttttggtcaa
    25561 ggtggagtac agactttgga tggagctgct catcaccatc ggaaaaatat ttttatggat
    25621 ctaatgacac cggaaagaat ggaagattat catcgtatct tagataagaa tctgacacaa
    25681 gcacttgaag cacagcatgg tcaatttgaa ctatttgatt taagtaagat ggttttcttt
    25741 acatctattt gcgagtgggc aggtatcaat ttatcagcga tttctaaaga tgaggtcgaa
    25801 aaattagcag aatatcaaat ctctatgatc agcggaacgt ttacttctcc gattgatcac
    25861 ataaaaggtg tcgagaatcg caaaaaatca gaaaaatggg cgcaaggctt gattgaagaa
    25921 gctcgacaaa atcctgtggc tggaaaagaa aatgtggcat tatatgcttt tgccaatgcc
    25981 acagatttag atggtcagct tttacctctg gaagtggcag ctgttgaatt attgaacatc
    26041 atccgtccaa cggttgctct aactgtgtgg gcggcattga tgggacatgc acttttcagt
    26101 agaccagatt tgtatcagca attaaaaaat gacttttcga cgttacaaga tccgtttatt
    26161 caagaaatga gacgctatta tcctttcttc ccaatgctac ctgcgatttc gctgaaagaa
    26221 gtagaagtag atggttatcg tataccagaa ggcagttggg ttattttaga tttatatgga
    26281 acagaccatg atgaacgaac agttgaagcg ccagattcct ttatgatcaa acggtatgta
    26341 gggaaagcaa aagatatttc ctataaagaa gaatatgaga tgattgcaca aggcggggga
    26401 aattttcgac aaatgcatcg ctgtgcagga gaatggatca ctcttcatag tctacgtgtg
    26461 ttttccgatc agctagtaaa taagtttgag ttctctgtac ctgaacaaga ttggacgatt
    26521 ccattcaatc agtttccgac ttatccgaat agccgagcgc tactttacaa gaattagatt
    26581 agcataaact tatcgaaaaa aaggaagatc aaacagtgct gatcttcctt ttttcatatt
    26641 actataaatt gataaggtca attgatcaaa atccacgaaa tgcttcatca atggtttgat
    26701 aatcttcatt ggataaatgc aaatctaaag cttggacatt gtcagatact tgctctggac
    26761 gcttagctcc aggtataact acagacactc gtggattctt catgtaccaa gctaaaacaa
    26821 gttgcgcagg agtggcttgg tatcttttag caatggggcg aagtacatcg acagcggtta
    26881 aaatttctct gaaacgttct ccttggaaat ctggatttct ttttcgagga tctccttcac
    26941 caaattgttt ttcctcacca agttcatatt ttcctgtcag caaaccggaa gcaagaggaa
    27001 aatagggaac aaaactgatt ttgttttttt caagataagg gaataattct ttttcagcct
    27061 gccgatggat caaactgtat ttatcttcta ctacatcgac atatccatct gcattcgctt
    27121 ctttcaattg ttctaacgtg aagtttgaca caccgactgc tcggattttt cctgcttctt
    27181 ttaattcgtg caatgtagca actgattcat tttttggtgt gctttcatcc ggaaagtgga
    27241 tataaaaaat gtcgatgtaa tcagtttgca gacgttttag tgcatcttcc accgattgtt
    27301 tgagaaactc aggagaatta tcaaatgttc gccctttatt tggtacatga gcagcttttg
    27361 tagcgatgac gacacgtgag cgatcatatt ctttttcctt cagtacctcg ccaatcagtt
    27421 cttcggaacg gccgtttccg taagcataag cggtatcgat caaattgatc ccgctattca
    27481 aagctgtacg aactacttgt tttcctgtct catcttccaa accgtcaaaa agattatgtc
    27541 ctccaacggc atttgctcct aatccaagct gttctgcgta gacttgtgaa tgtccgattc
    27601 ttatttctgc catagctgat tcctcctttt tgtctagtct aaaacaaaat accaaaaagc
    27661 tgaactaaaa cgtttttgtg aggggaaata gtcagatatc tcttgacttt gagactggtt
    27721 tttagtaggt tataagtaat atcgaatgtc aatgaaagag cgagtaagtt tttgaaactt
    27781 tacaagagag gatcatcgag gctgaaaatg atctaaagtt tcattagcct aaagacactc
    27841 tggagatgga cgagtgaaaa gccgtcccgc gtacgagcgt tattcgtaag aggaaagaga
    27901 aatctttcaa aattaggtgg taccgtgtat gaatttcatt cacccttgtt catgatgaac
    27961 aagggtgatt tttttattta catagctaag aaagggagag aaaactatga gtttccaaag
    28021 accaaaagga acaaatgata ttttgccagg cgaatcagaa aaatggcagt ttgtcgaaga
    28081 aacagcacgt ttattattca gagattatca gtataatgaa atgcgtacac cgatttttga
    28141 acactatgaa gtgatatccc gaagtgtagg ggatacaacg gatatcgttt caaaagagat
    28201 gtatgatttt tatgacaaag gggaacgcca tgtgacgctt cgaccagaag gtacagcacc
    28261 aatcgttcgt tcttttgttg agcataaatt gttcggccca gagtatgcga aaccgttcaa
    28321 aactttttat atgggaccaa tgtttcgcta cgaacgccct caaaaaggac ggttacgtca
    28381 attccatcaa atcggagtag aagcatttgg atcagaaaac cctgcaactg atgtggagag
    28441 catggtgatg gcacttgatt tcttcaaaca gttggggatc acgcaaatcc ggctagtcat
    28501 caattcatta ggtgataaag aaacgcgtca agcctatcgt caagcattga tcgactatct
    28561 tttacctttt gaatcagagc tgagtgaaga ctctaaacgc cgccttcatg aaaatccatt
    28621 acgcgtattg gacagtaaag acaaacgaga tcaaaaattt gtggcagaag ctccatctat
    28681 cttagattat ctaagcgagc ctgctcaaaa acattttgat accgttgttt ccatgctgga
    28741 tgcattggct gttccttatg aaatcgacag taatatggtt cgcggactag attattacac
    28801 acatacgatt tttgaaatca tgagtgacgc accaaaaatg ggggcacaag caacgatttg
    28861 cgcaggcgga cgttatgaca atctagtgga agaactaggt ggtccttcaa cacctggctt
    28921 cggatttgca atgggtatcg agcgattact cttgactatg gaggcagagg aagtagttat
    28981 ccctgcattt aatgagttgg atgcttacgt ggtcgcactc ggagacgaaa caaatatcga
    29041 agcattgaaa gtggtccaag cgattcgaaa ctttggtttt tcagctgacc gcgacttcat
    29101 gaaccgtaaa gcaaaagcgc aatttaagac agcagataaa gcgaatgcca aattagtatt
    29161 ggtgatcggt gaagatgagc ttgctagtca aacgattaat gtcaaagcaa tggcaagccg
    29221 aaaagaaaaa agttttccat tagaagagat atatgaacat tttgatgaag tctatgatga
    29281 aatgacttta ttcgagtagg aggaaaaaaa taaatggcac aaagaacgat ctattgcgga
    29341 ttagtttccg aacaacaatt agaacaaaca gttacattaa aaggatgggt acaaaaaaga
    29401 cgagaccttg ggggagtaat ctttatcgac ctacgtgatc gtgaaggaat cgttcaaatc
    29461 gtctttaatc ctgaaatcaa caaagaagca tgggaaatcg cggataaatg tcgcagtgaa
    29521 tacgtgatcg aagtgactgg ggaagttaga agacgcggag aaaatgcagt aaatcctaaa
    29581 atggccactg gtgaattcga agtgattgca agtgaaatcg tgattttgaa tcaagcaaaa
    29641 accccgccat ttcaaattga agatgatcaa gcaatcagtg acgaaatacg tttgaaatat
    29701 cgttatttgg atctgcgtcg tccaaaaatg actaaaaaca tcaaattaag aaatgcgaca
    29761 acaaaagcga ttcgtcatta tttagatgat aatgactttc tcgatatcga aacaccttac
    29821 ctagggaaat ctacaccaga aggcgcaaga gactacctag ttccttcacg tgtccatgct
    29881 ggacatttct atgcgttgcc gcaatctcct caattgttta aacaattgct gatgaatgct
    29941 ggattcgacc gttattatca aatcgttcgt tgtttccgcg atgaagattt acgtggagac
    30001 cgtcagcctg aatttacgca agtagatatt gaaacaacgt tcttaacagc agaagaaatc
    30061 caaacttaca cagagggatt gattgcgaag gtaatgaaag aagttcgcgg tatcgaagtc
    30121 acattgccat ttcctcgtat gacctatgat gaagcaatgg ctcgctacgg cagcgacaaa
    30181 ccggatacac gttttgatat ggaattgatc gatctaagcg atacagtcaa agaagtagaa
    30241 ttcaaagtct tccaaatggc tcttgaaaac ggcggtgtcg tgaaagcatt gaatgctaaa
    30301 ggcgctgcag atcgctactc acgaaaagac atggatcagt taggtcaata tgtaggacaa
    30361 ttcggagcta aggggcttgc atggctgaaa gtagaagaag acggattgaa aggtccaatt
    30421 gctaaattca tgggagaagc aacagaagca atcattaaag caacagatgc aaaaccaggt
    30481 gacttgctga tgtttggcgc agataaaagt gaaatcgtcg ctgctgcatt aggtgctatc
    30541 cggacacgtt taggaaaaga attaggacta attgatgaat cgaaattcaa tttcttatgg
    30601 gtaactgatt ggccacaatt tgaattttca gaagaagaag gtcgatatgt ctctgctcac
    30661 catccattca cgatgccaaa agaagaagat atcccgttat tggctgaaga ccctgcaaaa
    30721 gtctatgcag aagcttatga tatcgtcttg aacggttatg aattaggcgg aggttctatc
    30781 cgtattcata ctcgtgagct tcaagaaaaa gtactagaaa ctctaggctt tacaaaagaa
    30841 caaatggaag aacaatttgg tttcttgtta acagctttag attacggatt ccctccacat
    30901 ggcggtattg cattaggatt ggaccgtttt gcaatgttat tagccggaga agataatatt
    30961 cgtgaagtca ttgcctttcc taaaaacgga aaagcagcag atgtgatgag tgatgcacca
    31021 agtatggttt cgcctttaca attgttcgaa ttgaatattg atgtaacgaa tatagaagaa
    31081 tagccgataa aaaggtaaaa aggatttgtc gaatacgctt cttttgacaa ccttgattta
    31141 attgaaaaac actttataat gaagccataa catataaagc ggggcttttc catgaaaaaa
    31201 acattcgaaa attgggctta tcatgattac acgaccaagc tttcggtatc tatcgtttat
    31261 gcgttgatcg catcagtcgc attgaatttc ttttatcagc caggacatat ttattcaagt
    31321 ggtattactg gtctagctca gattttgacg actttgtcaa aatcactgat aggattcgaa
    31381 gtaccggtat caacaacttt gtatttactg aatatacctt tgtttgtatt ggcgtggtta
    31441 aaaatcggga agaagtttac aattttcact tttatcacag tcactttgac ctccctttgt
    31501 atccatattt taccggaaac agtcctatct accgatccaa tcatctgtgc gttgtttggg
    31561 ggggctgtga tgggactagg tattgggcta gcgttgagaa acggcctatc atcgggcggt
    31621 ctagatatcg tgagtatcac gattcgtaaa aaaacaggaa agtcgattgg ttctatttcg
    31681 atctacttca atgcattgat cgtttttgtt gccggaattc tatttggctg gcaatatatg
    31741 ttttacagta cgttgtctat tttcgtcagt ggaaaagtaa ctgatgtcgt ttatactaaa
    31801 caaaagaaga tgcaggtaat gatcgtaacg aaacatccag aaaaagtagt aaatgaaatc
    31861 caaaactgta tgcgtagagg gatcacgatc atcaatgaag cagaaggtgc ttataagcat
    31921 gataagcaga cagtattgat gacgatcgtt acacgatacg aactgcctct acttagagaa
    31981 gcaatgaaag cttctgatcc acaagctttt gtaagtattg cagaaaacgt ccagatactt
    32041 ggtcgtttct atgaggatgg catttagatc gagtttgaca aaaattcatg aaatactgct
    32101 gtagacagac gttcgtttgt ctgcagtttt tttctaccat agtagaatca gcaaagaaaa
    32161 actgcttttt tatttgaaat tttcttaaag ttgaaaaaaa gcatgtttat ttttttaaat
    32221 ttatgggatg atagaaaaaa atagtagaga aaggacacga ttctacttgc tattttcact
    32281 taatacccgt atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa
    32341 acaaaaaagg ttaaaaaaca gccgctacat cactgggttt gatgggctcc gttcattagc
    32401 tgtggtgggt gttatcttct atcaccttct gccaactagt atgaaaggcg gctatttggg
    32461 cgttccgatt tttttcgtcg tatctggtta tttgatcacg gatctgctgc ggcaggaatg
    32521 ggaacaaaat ggaaaaatca atatttggca attttatgtc agaagaatga agagactcta
    32581 tccggcactt gtttttatgc ttgtgacagc ttcggcttat atcactttgt ttcagcgagg
    32641 gctgttaaac aatcttcgag gagttgtggt cagcagctta ctgtatgtca acaactggtg
    32701 gcaaatcaat aacggtttat cttatttcga tcgatttgct aatgagtcac cgtttacgca
    32761 tatctggtct ttagcagtag aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt
    32821 gttgatggta tttgttcgta aaagaaaatg gatcttttat actgtgttag gtacatcctt
    32881 cctctccgcc atcttgatga tgatcttgta tacaccagga ggcgatccaa cccgtgttta
    32941 ttacggtacc gacactcgtc tgttttctat atggttggga agtgcgcttg cgtttgtttg
    33001 gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc
    33061 tggaggaatc tcgcttttgg cactgattct ttgcttcttc tttttagatg atcattacaa
    33121 tttcatctat tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc tagttgcagt
    33181 gacagcacac cccggagcaa gtttgaatcg atggctgaca aatccagtat ttacttggat
    33241 tggcaaacga agctatggca tttatctgta tcagtttcca gttatgattt tttatgaagc
    33301 aaaggtgact gatattgccg atcatgtttt gttgcatact ttagtggaaa tcacattgat
    33361 tctaatcatc agtgaacttt cttatcgata tgtggagcgt ccgctagcac gttttgatta
    33421 tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa
    33481 accatgggtt gttccaggca cattacttgt agcaattgca ctcgttggtt tcgttacggc
    33541 accgaaaaac gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa
    33601 aaaattagca gaagaaacca gaaacaattc agaaaacacc actagttcgg ttgaccaagc
    33661 gatccttgat aagtacgagt tgtccaaggc acaagggaat aaggcacaaa aattagaact
    33721 aacggcattt ggtgattcag ttatgttgga tgctgcaacg gatctacaag aactttatcc
    33781 aaaagtagtg gtagatggag atgtcggacg acaattatat gccagtgtac cgtacattga
    33841 aaaattaaag gaacaagatt tgttgaaaga cactgttctg attggactag gaacgaatgg
    33901 ctcattctcg gaagctcagt ttgatagtgt gatggatgca atcggtgatc gaaaagtata
    33961 ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga
    34021 acagatgacg aaaaaatacg acaatatgac gctgattgat tggtacagct acagtaatga
    34081 ccatagtgac tggttttatg atgatcaagt ccatccaaat cctgaaggga tgacacatta
    34141 cattcactta gtcagcgaag ccttgctagg aggaagttca agtacgaaca gcagtgagac
    34201 aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa
    34261 gtttttttat atttaccgtg tttttaacat tcatatagat tcaggaagca aaacggttga
    34321 gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt tttttgaatg cagcttctgc
    34381 ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa caggtcttct
    34441 tttttgaaga cgagtatatt tagcaccagt accattgtta tgttcctcta atcgtctaga
    34501 aagatcggtt gtatatccac cataaaatgt ctgatcttta cagaataata cgtaaaaata
    34561 atgttcattt gccatagagc atctcacgaa tctctgcagt gtattctcca tccatattgt
    34621 aagtgatcaa tggtggagcg actttgaatc cgtctaattt gccttctttg attccttcaa
    34681 ctaacaaaat atttgcctct tttccaggtt ttgggtaaac aaattggatc cttttaggtg
    34741 caattcgata gtcttccatg acctgaatga tttctaagaa ccgatcagga cgatggacca
    34801 tagtaaagcg tccatttgtt tttagtagct tacttgaaat atcgatcact tcttctaacg
    34861 tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt ttatttgttg
    34921 gcagcccttt gaaataaggt ggattacaga cgactaagtc acaagaatta tgtcgaacga
    34981 cagataagct gtctttcaga tcgatggtgt ggacagtcat ttgttcattt aaatggttaa
    35041 gcgcgatact acgtcttgcc atgtctgcta gacgaggttg aagttcgata gcgtcgatag
    35101 ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa
    35161 caatcttccc ttttttaggt acacgggaaa agtgagcaag cagcacagca tctaaagaaa
    35221 aagaaaacac tttggaactt tggataatct tgatatcttc tgcgtataat tggtcgattc
    35281 gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt ctgtattata
    35341 ctatttttat taaaaagaac caaggtggct tgtcaaacct ctatttttta tgcatactat
    35401 gaccataaga agggaaagga agagactatg ttttatcgat ttatgcgcgg ggtagttcga
    35461 gtactattag ccttagttaa tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa
    35521 gatgagaatt acatactagt tgctccccat cgtacttggt gggacccatt atatctggcg
    35581 gtagctgcag cacccaagga attcagcttt atggcaaaag aagaactgtt caaaaatcca
    35641 gttctgcgat ttatattaac tcgatccaat gcttttccag ttaaaagaga aaatccagga
    35701 ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag tttgatcatg
    35761 tttcccagtg ggacaagaca tgctacagaa cttaaaggcg gaatggcatt gatttccaga
    35821 atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc cgttgacact tggggatctg
    35881 ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag
    35941 aaaacggata aagaaggtat ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa
    36001 ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata aaatgataag
    36061 tttatgcgtg ggcggtcttt tgaatctact gtcctgttca gttcagactt tttcaatgaa
    36121 aacgagggag agacgtcaag tctgccctct ttttgcgttc caatatgtaa aatagaagac
    36181 aggtgtcatg taatggatca aagtgttata ataattagaa ggacattttt tgaggtgatt
    36241 gaatgcgttt tttacataca gccgattggc atatcggaaa aaaactacaa ggttatgact
    36301 tgattgaaga ccagcgtcat gtactatctt ctatactaga tatcgcgaaa gaagaaaaag
    36361 tagaggcaat cgtgattgcc ggagacttat atgataggag tgtcccagct gttgatgccg
    36421 tagaattata caatgaattg atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg
    36481 ctatatcagg aaatcacgat agcagtacga gactttcagc gggtacgcca tggttcagtt
    36541 attcgaacta ttttttgcga acacagctag cagaagcatt tgaaccagtc gagtttaaaa
    36601 acgtacaatt tttctttctt ccttactttg aaccaattgc agcgagatta tattttgaag
    36661 atgacaatat ccgaacgatc aagcaagcgg tagccaaggt gatagataaa atgcaggagc
    36721 tatttgattc agaaaaaaaa caagtattga ttagccattt ttttgtaatg ggaagtgtaa
    36781 aatctgattc ggaaacgaaa atcgaagtag gcgggttgga ttcgatccct ggtgaactac
    36841 ttacaccgtt tgattatgta gcactaggac atttgcataa tcaagctgct ttgagacagg
    36901 ataatgctcg ctatagcggt tccccattga aattctcttt atctgagatc aaccaaaaaa
    36961 aaggcgtatg gatcgttgac ctgacaacag gcctggagtt gaattttagg gagatcgaac
    37021 ccctgcatga tattgaagaa ataacagcga gtttcaaaga attgctggat cctgcctttt
    37081 atcagtccgt caatcgggag aattacatct atatctatct cactgataga acgatcatcc
    37141 cgaatatgat gaaccagctt cggcaaatat atcctcgtat tttggggatt gaacgggcaa
    37201 acggtcgagc agaacaaaca aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa
    37261 aattagcaga agcatttttt agtgaaatga cggaagagca gctaactgca aaccaagaaa
    37321 aatggctcgg agaaacgttg caagaaatca tggaaatgga atgatcaaga atgaaaccga
    37381 aaaaattaca acttaaaaat tttggtcctt ttatcaatga gaccgtagat ttttcacgtt
    37441 tgacagaagc accgcttttt ttgatcagcg ggaagacagg agctggcaaa acgacgattt
    37501 ttgatgggat caccttcgca ctttttggtg aaacttccgg aagacttcgc tctggaaaag
    37561 agatgcgttc tttgtttgct acaccagacg aagaaacaag cgtgactttt tcttttgaac
    37621 atcagcagat gagctatgaa atcgaacgaa aaccagaaca agttttagca aaacgaaaag
    37681 gaaacggaac aaaaaaacag gcagccaaag taacgttgac catttttgat gggaaaggaa
    37741 aagaaataaa acaaatcaca aaacgtacag aagcagatca gttgatcaga gaactgatga
    37801 atctcgatgc gaaacagttt tctcaaatcg ttttactacc ccaaggagaa ttccgtaatt
    37861 ttttggtttc atcaagcagt gataaagaga ctgttttacg tcatttattc ggtactcaat
    37921 ttttccaaca gttcaatgag cgattaaaag aaaaagcaaa acaacagcaa caaaaattgg
    37981 atcatttaga gcaagagctg catcttctgc aaaaaaggtt tgttttagtc aaagaagagg
    38041 aaccagcagc agggtttgaa gccgttttag cccaatggac aaaccatcag gcaatactcg
    38101 aaaaacaaat cacggaagaa aaagaagctc taacggaact tcaggaaaaa caaaagcaac
    38161 tagaaaattc ctactatcgt tttgaaagct tgaaaaaaag ttatgaagaa aaaacgatat
    38221 taataaacaa gcaaaaggaa ttagctgctc aaaaagacga aatagaggaa aagaaacgat
    38281 ggattgggta ttatgaattt acgcaacgat tagtcgaacc aatccagcgc ctacaagaaa
    38341 taaaagttga aaaaatagaa ctgtcacaaa aaagtagtga gcaaacaaag aaactagcga
    38401 tgactacgca gaattatgaa gagtggcaaa aaaatgaagc aaaccgcgta cgccgacaaa
    38461 caaaaatcaa agaagctgct caaaaactgc aaaacaaaca gatgatggtg ccaattgtcg
    38521 aatccttacg tagtaaaaaa caagacgcag aacaattaac aatttcattg gataaagaag
    38581 aattgaatgc cgagaagctg tatcaaaatc tcgctatcta tcaggaaaga actcgaattc
    38641 ttcaagaaaa tttactggat caggaaaaac tgcaagaaga tcgtcttgct tttggacagt
    38701 taaaatatct agatgaaaag cagcaagaaa tagaaaaaca aaaagcaaaa gacactgggt
    38761 cgctcgcaca gataagagaa gaactcaaaa aagcaactga agaaataaca aaaaatagtc
    38821 agctattgaa agaacagaca gaacaagcag aacagctgaa aagccaatgg gcaaagcagc
    38881 aaattgctcg tcttcagctt tttttgcgtc caggggagcc atgtcttgtt tgtggatcaa
    38941 cggaacaccc catacaacaa ttaaaacacg aacagccaaa taaggaagat atcttaaaag
    39001 tagaacaaca actggcagaa gctgagtctg ctgtagaaag aactcagcaa atcattgcca
    39061 aaagtgaagc acatgaaaat aatcttcaac aacaattatc agatttagaa caaaaaatca
    39121 tggcaacgaa tatacaattg gctgaacaag aagaacactt gcaacaggct ttgaaaaagg
    39181 catttcctga gataacagaa caaaaaacaa aaacaactct tgtagtaatc gaacaatctt
    39241 tagatagacg aaaaagcaag atggccaatg caagaataga gctggaagaa ataagacaac
    39301 aagcagaaaa gctcgaacaa caattagctg tctacaaaca gcagcagaca gaaaaaaata
    39361 atcagctcca gcaactgcaa ggagaaatca aaagtttaga acaacaattg caagatacgg
    39421 atagtaactg tttagaagag gagatacgac gacttgaaaa acagttagaa gaagactcga
    39481 atcaactagc tgttgagcag aaagcaggag tgcaattgtt gcaagatctt catacgcttc
    39541 ggacacagct tgatcttcaa catgaacaaa tagaaaaaat agatgaaaaa atagaaaagg
    39601 aacaagaaag aatagctaaa aagttagaaa gccagtcgta tttttcaaca attgaagaaa
    39661 tctgtgcatt tgcacaacag caagcacaat ataaagaaaa ccaacgtaca gttacttctt
    39721 atcatgaaga gcaattagtg acagctgacc ggctgcgtca attagaaaat atccagctaa
    39781 ctgagacatt cccagataca gaagagatca gccaacagtt agaagaaata agacgtatga
    39841 tcgaaataaa gcaagaaaag atatacggtt tacaagaaca aaaaattagt aatcaaaaaa
    39901 tctacgatga atgtctttcg atttatcaca caagccagac gaaattagat gagcttacgc
    39961 aattacagca gctttcgcaa acattcaacg gagaaaatcc taagaagact agtttggaac
    40021 ggtatgtgct tcaggtttat cttcaagaag tgttacaagt agcaaatgat catctccaac
    40081 gtttaacgaa aaatcgttat caatttgagc tagcggatac cattggcagt tatcgaggga
    40141 aaaccggatt agaaatcaat atctatgatg atcaggcagg tatgtctcga agtgcgcata
    40201 ccctgtctgg cggtgagagt tttattgcag cactttcttt agcattagct cttgccgatg
    40261 tgatccagac acaagctggt ggtgtagcaa tcgatgcatt gtttattgat gaaggatttg
    40321 ggtcattaga tgaagaagct ttggaaatgg caatggaagc tttagaaacg atcgaaagtg
    40381 aaggaagaat gatcggcatc attagtcatg taagagaatt gaaagaacga gtattacaac
    40441 aaataagaat tgatacagaa ggtagcggac aaagcaaaat acgctatcaa cttggataaa
    40501 aagggagatt ggtaactatg aaatggagta ttccagaaaa agtcattgca cgtggacgtc
    40561 aatacttgca agaaggccga gtgttatcgg ttgtccctga ccaagaagaa aacgtgtggc
    40621 atgcagaagt cttgggcagt gaattgtata tggttgattt ggatggtaca gcaaaagaga
    40681 tagattactg ccaatgtccc tattgggagg aacataaata ttgtaagcat acagttgctg
    40741 tggaacttta tttacggtcg aaaaatctat ctcgtatcat gaaaaaagac cagccaatgc
    40801 tagaaccggt aaaagcaaca agtgagggag aaatcattgc aaaaggtttt tctcgtctaa
    40861 aaacacaaaa caatcctcaa gcggtgcaac ctttgattat tgaataccaa gtagaaacga
    40921 ttgagactaa tcagtatcac ccagagcttt ctattttggc actttacttg cgtatcggtt
    40981 cattggcatc accgaaaaaa acatatatcg taaaaaacat ctatgatttt ttacaagctt
    41041 acacagaaaa agaaacttac actgttaata aacagtacca gtttcgtcta gataaacggg
    41101 cgtttcaaaa agaagatcaa aaaatactca cgcatcttgc aggaagtgct cagacgcatc
    41161 aattattagg tacgaacggc ttacaagtca aaggaaaact agataaaaga tatctgcttc
    41221 tcccagtaga acaagcccgc ttcctgttag agcagatgaa tcagactaca cgtttgcatc
    41281 tgcagataaa tgaacaaaac gatcaaggaa tacaattttc cgatcaaaac aaacctttat
    41341 cattcaaagt ggaaaaattt ggagaagatt atttgctgca agcattgaat gattttgatt
    41401 ttttctttat gcattatcag tggggagtta tccaagggac attttattct ctgacgaaat
    41461 atcaacaaac ggtttatcaa acttggaaac aattgattcg ccgattggag aaaccagaag
    41521 tagtgttcaa gaaaaaagaa ttaccgtctt tgtttaaaga gattatccct ctgctgtcag
    41581 agatcggcga cgtagctatt gcccctgcag tagaagagga agtagcagat atccccgtcg
    41641 aatttatttt ctttttccga aaagcaaaag gaaaaatcca agctcgagtc gatttcgtct
    41701 atggtgaagt catctattcg actgacccga agcatgaggt ctcgacagaa caggcttttc
    41761 aggtgttacg tgacacagta gaagagaaac gagtacttga tctattcaga atgtatcgct
    41821 accagaaaaa tgaaactggt tatgaacgtc cattgccaag cggggaagaa ctatatgcct
    41881 ttttccgcac agaactatct gtcttccgtc agtttggtga agtcagactt ggaagaaaat
    41941 tgcgagagtt gtatttagat gcgcataaac atcaaccgaa aatcgaagta accgagaccg
    42001 gttcatggct cgatattcga ttcgatgtga cagggataga ggagcaagaa atcgatcatg
    42061 tcctgcaaag cttgctgaga aatgatgctt tttatacatt agaaaatggt caggttcttt
    42121 cttttgattc agaagaattt caacaaacaa gccaagtgtt gcaacaatta cgggaatcga
    42181 tacgtacaga agaaggaacg atccatgtac ctaaaaatca aggattgatc attcaaaatc
    42241 aattggaaaa aagcaatgct acttttagtg aatcgttcca aacgatggta caagatctca
    42301 ttcatccaga aagataccag gcacagcttc ccaaaggatt gaatgcaacg atgagagatt
    42361 atcaaaagca aggttttcga tggttgaaga tgttaggtca ttatcagttt ggcggtattt
    42421 tagccgatga aatgggactt gggaaaacac tacagacaat tgccttttta ctttcggaaa
    42481 aagaagaacg gaaaagcttc tctgctttga tcgtagcgcc tgcgagctta atttacaact
    42541 ggcaggcaga ggtgagaaaa ttcgcccctt ctttaagtat ccaagtaatc aatgggaata
    42601 aaaaagaacg tgaagaatta ctggctaaag acacagatat ccgagtgact tcttatgcca
    42661 gcttaagaca ggatctagct aattatcagt cgcaaaaaat tgattatttg attcttgatg
    42721 aggcacaaat ggtcaaaaat agcagtacga aaactgcaca agcattgaga gaattagctg
    42781 tacctcagcg gtttgcactg agtgggacac caatcgagaa taatttagaa gaattgtggt
    42841 cattgtttgc aacgattatg ccaggatttt tcccgactaa aacaaaattc agggaacttg
    42901 ccgcagaaga aattgcgcaa atgattcgac cgtttatttt acggcgagat aaacaaactg
    42961 tgttgaaaga cttgccggaa aaaacagaga tgaatctata ttctgcactt acagaagaac
    43021 aaaaaactgt ttatttagct tacttgcgtc aaatgcgtga agaaatcaca tcaatggatt
    43081 ctgaagcatt caagaaaaac cggatcggga tacttgctgg tctgacaaga ctgcgacaaa
    43141 tttgctgcga tcctcgtctg tttattgaag attaccaagg cggttccggc aagctcgaac
    43201 aagtaaaaga tctactggta gctgccaaag agaacaaacg aagagttttg ctgttttctc
    43261 aatttacagg gatgctgact atcttgcaag aagagttagc cgagctaggg atctcgacat
    43321 tttatttaag aggcagtacg aaaccacaag atcggttaag tatggtagac gcatttaatg
    43381 ctggcgaaaa agatgttttt ctgatttcct taaaggctgg gggaactgga ttgaatctca
    43441 cgggagcaga tacggtcatc ttgtatgacc tttggtggaa tcctgccatt gaagaacaag
    43501 ctgctggacg tgcacatcgg atcggacaaa aaaatgtggt agaagtgtgg cgtatgatct
    43561 ctgaaggaac gattgaggaa cgcatggatt cactccagca agaaaaacgc gaactgttcc
    43621 aaaaagtgat ccaaggtaat gaagaacagt tgacgaaact gactgaagaa gacatccgca
    43681 tgattttaag tgtaggagaa atcgaggagt aagagactcg aaaaagcgtt cagtaatggg
    43741 taagaaagcc tttctaaaga agcgattgtt tatttgattt aaaaaatagc aaggaggctg
    43801 ggattccatt ttatcccagc ttccttgcta tcattttttt atttctattt ttaaaggtta
    43861 tacaaagatt gtcttcattg tctctaacag ctgatcaaaa gaagaaatct cgatatcgca
    43921 aattggagtc tgttcaattt tgcgttctcg atggttgaac caaagcgctt gccagttagc
    43981 actttttgca cctagaacat cattgtcgta attatcgcca acgtataagg tctcttctgg
    44041 aagcatatga aattctttct cagccagctg aaaaatctct ttttctggtt tttggaaacc
    44101 tgttgcttga gaaatgagca tattgcggga aggaatccaa ttgttcaatt gaagttgctt
    44161 cagttttttt gtctgatggt cagtcggccc atttgtgata attcctaatg ggacattctt
    44221 ttccttcaag aaatcgaggg tttttttgac ctcctcatgc aagcagatat tatcaagttc
    44281 ttcctcgtaa attttttgaa ataagaggcc atcttcttcc gtaatatgag ggtagtctaa
    44341 atcttttagt gattggctga tccgatgtgc tcgcatatat tctagtgtcc aatctcctgc
    44401 cattactttt gggaagtttt catcactatg atggcggaac cggatatata gtggatgcat
    44461 atctgcatct gaaacaagcg gaacgactct tttgaccgcg tttcgaaatg gctgctgctg
    44521 atcatacatc gtatcatcaa catcaaacac aactgattta atcaaaattt tacacgcctt
    44581 tcggttttca ttgatttttt cacaaatcct tgactattgt aacagaaaaa attgtctaaa
    44641 aaagctgata aataaaggtt tttcttacta tatttctatt tccttgacag aataaggttt
    44701 ttatatttca gtttttttca ctaaaaatcg tgaaacacgt caataagttt gatgaacaaa
    44761 ttaatcatta tggtagaagt cgattgattt attttaatgc aatctctgtg gaattttcga
    44821 aagatttatt tttttaatac agtctcaata taactattca aa
//
LOCUS       DAZUMT010000010        43672 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000010 DAZUMT010000000
VERSION     DAZUMT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.010"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..907)
                     /locus_tag="ACG8FM_000500"
     CDS             complement(32..907)
                     /locus_tag="ACG8FM_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5093190.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1055..1387
                     /locus_tag="ACG8FM_000501"
     CDS             1055..1387
                     /locus_tag="ACG8FM_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5093191.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1499..2707
                     /locus_tag="ACG8FM_000502"
     CDS             1499..2707
                     /locus_tag="ACG8FM_000502"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS5093192.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2861..3316
                     /locus_tag="ACG8FM_000503"
     CDS             2861..3316
                     /locus_tag="ACG8FM_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093193.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3678..3953
                     /locus_tag="ACG8FM_000504"
     CDS             3678..3953
                     /locus_tag="ACG8FM_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093194.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3966..6044
                     /locus_tag="ACG8FM_000505"
     CDS             3966..6044
                     /locus_tag="ACG8FM_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5093195.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6207..8090
                     /locus_tag="ACG8FM_000506"
     CDS             6207..8090
                     /locus_tag="ACG8FM_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5093196.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            8102..9049
                     /locus_tag="ACG8FM_000507"
     CDS             8102..9049
                     /locus_tag="ACG8FM_000507"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5093197.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9069..9596
                     /locus_tag="ACG8FM_000508"
     CDS             9069..9596
                     /locus_tag="ACG8FM_000508"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5093198.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9659..10312)
                     /locus_tag="ACG8FM_000509"
     CDS             complement(9659..10312)
                     /locus_tag="ACG8FM_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS5093199.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10444..11286
                     /locus_tag="ACG8FM_000510"
     CDS             10444..11286
                     /locus_tag="ACG8FM_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5093200.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11444..12340
                     /locus_tag="ACG8FM_000511"
     CDS             11444..12340
                     /locus_tag="ACG8FM_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5093201.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12343..13056
                     /locus_tag="ACG8FM_000512"
     CDS             12343..13056
                     /locus_tag="ACG8FM_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS5093202.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13076..13594
                     /gene="msrA"
                     /locus_tag="ACG8FM_000513"
     CDS             13076..13594
                     /gene="msrA"
                     /locus_tag="ACG8FM_000513"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5093203.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            13591..13818
                     /locus_tag="ACG8FM_000514"
     CDS             13591..13818
                     /locus_tag="ACG8FM_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS5093204.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            13970..14521
                     /gene="lepB"
                     /locus_tag="ACG8FM_000515"
     CDS             13970..14521
                     /gene="lepB"
                     /locus_tag="ACG8FM_000515"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5093205.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(14604..15146)
                     /locus_tag="ACG8FM_000516"
     CDS             complement(14604..15146)
                     /locus_tag="ACG8FM_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5093206.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(15383..15781)
                     /locus_tag="ACG8FM_000517"
     CDS             complement(15383..15781)
                     /locus_tag="ACG8FM_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5093207.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            16041..16901
                     /gene="ylqF"
                     /locus_tag="ACG8FM_000518"
     CDS             16041..16901
                     /gene="ylqF"
                     /locus_tag="ACG8FM_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS5093208.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            16894..17661
                     /locus_tag="ACG8FM_000519"
     CDS             16894..17661
                     /locus_tag="ACG8FM_000519"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS5093209.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            17717..18574
                     /gene="dprA"
                     /locus_tag="ACG8FM_000520"
     CDS             17717..18574
                     /gene="dprA"
                     /locus_tag="ACG8FM_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS5093210.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            18696..20774
                     /gene="topA"
                     /locus_tag="ACG8FM_000521"
     CDS             18696..20774
                     /gene="topA"
                     /locus_tag="ACG8FM_000521"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS5093211.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            20743..22119
                     /gene="trmFO"
                     /locus_tag="ACG8FM_000522"
     CDS             20743..22119
                     /gene="trmFO"
                     /locus_tag="ACG8FM_000522"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS5093212.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            22317..23222
                     /gene="xerC"
                     /locus_tag="ACG8FM_000523"
     CDS             22317..23222
                     /gene="xerC"
                     /locus_tag="ACG8FM_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS5093213.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            23259..23807
                     /gene="hslV"
                     /locus_tag="ACG8FM_000524"
     CDS             23259..23807
                     /gene="hslV"
                     /locus_tag="ACG8FM_000524"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS5093214.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            23821..25221
                     /gene="hslU"
                     /locus_tag="ACG8FM_000525"
     CDS             23821..25221
                     /gene="hslU"
                     /locus_tag="ACG8FM_000525"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS5093215.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            25239..26030
                     /gene="codY"
                     /locus_tag="ACG8FM_000526"
     CDS             25239..26030
                     /gene="codY"
                     /locus_tag="ACG8FM_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS5093216.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            26112..26987
                     /locus_tag="ACG8FM_000527"
     CDS             26112..26987
                     /locus_tag="ACG8FM_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS5093217.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27119..27757)
                     /gene="plsY"
                     /locus_tag="ACG8FM_000528"
     CDS             complement(27119..27757)
                     /gene="plsY"
                     /locus_tag="ACG8FM_000528"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS5093218.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            27884..28318
                     /locus_tag="ACG8FM_000529"
     CDS             27884..28318
                     /locus_tag="ACG8FM_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS5093219.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            28484..30529
                     /gene="parE"
                     /locus_tag="ACG8FM_000530"
     CDS             28484..30529
                     /gene="parE"
                     /locus_tag="ACG8FM_000530"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS5093220.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            30541..32991
                     /gene="parC"
                     /locus_tag="ACG8FM_000531"
     CDS             30541..32991
                     /gene="parC"
                     /locus_tag="ACG8FM_000531"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS5093221.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            33268..35499
                     /gene="pflB"
                     /locus_tag="ACG8FM_000532"
     CDS             33268..35499
                     /gene="pflB"
                     /locus_tag="ACG8FM_000532"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS5093222.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            35743..36504
                     /gene="pflA"
                     /locus_tag="ACG8FM_000533"
     CDS             35743..36504
                     /gene="pflA"
                     /locus_tag="ACG8FM_000533"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS5093223.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            36589..37515
                     /locus_tag="ACG8FM_000534"
     CDS             36589..37515
                     /locus_tag="ACG8FM_000534"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS5093224.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            37697..38590
                     /locus_tag="ACG8FM_000535"
     CDS             37697..38590
                     /locus_tag="ACG8FM_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS5093225.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            38724..38849
                     /locus_tag="ACG8FM_000536"
     CDS             38724..38849
                     /locus_tag="ACG8FM_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS5093226.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(38983..39615)
                     /locus_tag="ACG8FM_000537"
     CDS             complement(38983..39615)
                     /locus_tag="ACG8FM_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093227.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(39763..40305)
                     /locus_tag="ACG8FM_000538"
     CDS             complement(39763..40305)
                     /locus_tag="ACG8FM_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS5093228.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            40440..40829
                     /locus_tag="ACG8FM_000539"
     CDS             40440..40829
                     /locus_tag="ACG8FM_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093229.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            40833..41597
                     /locus_tag="ACG8FM_000540"
     CDS             40833..41597
                     /locus_tag="ACG8FM_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5093230.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            41726..42673
                     /locus_tag="ACG8FM_000541"
     CDS             41726..42673
                     /locus_tag="ACG8FM_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5093231.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            42803..43543
                     /locus_tag="ACG8FM_000542"
     CDS             42803..43543
                     /locus_tag="ACG8FM_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093232.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT        15201 a         7502 c         9138 g        11831 t
ORIGIN      
        1 ctttcctcat agcctatctt tgtctttttt cttatagttt tttcagtaat cttgtctgtg
       61 gcttcaaata agtaaagcct tgcccttcca cttcatgcac attgatgatt gaaaggaaag
      121 ctttttcgtc aatctcgtgg acgatcctct tagcttcagt taattcacta gagctaacga
      181 ccatatagat gatttttttt gaattaccag agaatgcgcc ttctccagaa aggaaagtca
      241 cacctctttg cagaccggtc atcagtagtg gtgcaatctc ttcgcttttg ttggagacga
      301 caagtagacc ttttgcagaa tatccgccat ctaagatcga atcaatcaca cgactgaaaa
      361 catacgaaac aatcaacgta tacatcatac gtttcaagtc gatataggtt aatgaagcaa
      421 gtaagacgat gatatcaaat gccaataggg aacgccccat agtgatgccg taattttgtt
      481 ctaaaatccg agcgataata tcactgccgc ctgttgttcc acccatgcgg tagacgatcc
      541 cgcttcctac acctgcaact aaacccgcaa gtagagaaac gatcagtaga tcatgctgta
      601 aattgatttc tagtggaacc ctttgccaga tccataaaaa gaatgacaaa gaaagtgtac
      661 ctagaacagt ataggccaag gaacgtttac cgaggatctt tcctcctagt aaaatcaatg
      721 ggatgttgat taataaagtc gtataagctg gatcaatacc aaataaagct cttaagatca
      781 aggtgatccc tgagactccc ccttctgcta aatgattagc gatgttcaag taaacaagac
      841 caaatgcata aatacttgtt cctataatga taaaaagaac ttcttttact gtttttcgtt
      901 tgaccatatg aattccagct ttcaattatg tattttcaca gaatttccat ttagaaattc
      961 cttcctaaat gtagcatgga aattcgattt tctcaattgc tcttatctag gatttgcgtt
     1021 aaagttttgg taagatagtt ttggagggaa aaagatggat aaaaagcgtt ctttatacag
     1081 tatgcagcag gaagtggatg aatatatcca gcaattcaag gtggggtatt tctctccatt
     1141 agctcagatg gctcgattaa ctgaagaagt aggtgaattg gcaagagaag tcaaccacac
     1201 ttatggagaa aaaagcaaaa aggcttctga accagttaac tcggtagcag aagaacttgg
     1261 tgatgtttta ttcgttacga tgatcatggc taattcattg aatatcgact taactgaagt
     1321 ttttgaaaaa aacatggaaa aattcaatca aagagaccat aatcgctttg agcgtaagga
     1381 cgagtaaaca agatgaagtg gtcctggcct tgaagactct gaaacggact tgtcaaacag
     1441 tcaccatgca actgagtctt tatctcagga caagaaacac gcaggaaagg ttggaaaaat
     1501 gaaattaaaa caattacctc tggaatacca aaaggcaatt ccagttctga aaaaattaga
     1561 gaatgcagga tatgaagctt attttgtagg tggaagtgtt cgtgatatct tgctgaacca
     1621 gccaatccat gatgtggata tcgcaacaag cgcatttcca gaagaaatca aacagctttt
     1681 tccaaaaacg atagatattg ggatcgaaca tggaactgtg ttggtattag aaaatgatga
     1741 acaatatgag atcaccacat ttcggacgga atcaacttat caggactacc gtagaccaga
     1801 tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa agaagagatt ttacgatcaa
     1861 tgcgtttgct ttgaaagaag atggagaaat catcgatttg ttcgatggtc tgcaagatct
     1921 agaagaacag atcttgcgag ctgtaggcaa tcctcatgag cggttccatg aagatgcctt
     1981 acgaatgatg cgcgggttgc gttttgtcag tcaactaggg ttcaaattag aggaagaaac
     2041 cttctacgcc atcaaagaaa accacgagct attaggtaaa atatctgtag aacgtataaa
     2101 tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc gcgggaatcc atgcatttat
     2161 tgaaacagag tgctacattt gctgtcccgg tttgaaagaa gaaggcgaag cacttttacg
     2221 ttttgctgaa ttaccatccg tcccattgaa agaagagagc caagcatggg cattattgat
     2281 cagccaactg gatctaaaag aaaatgagat tcgttccttt ttgaaatcgt ggaagtgttc
     2341 taaccaaatg attaaagacg ttcaagcact tgtccaagga ctacgacaac gagaaactgg
     2401 agctttggaa ccattcgaat tgtacttctt aggcaaaaaa catgcactgc aagtagaaga
     2461 attgatgatc tattttgacg aaacacctca gatggatgcc gtaggagaat cctatgatcg
     2521 tttacctatc aagtcattaa gcgaattagc agtagatggt aaaatcttgc tagaagaaac
     2581 tggaaaaaaa cctggtaagt gggttagtga agctttgcaa ttagctgaaa aagccgtcgt
     2641 agaacaaaga ataaaaaatg ataaaaaaca ggttttggat tttttatcga aagaaaaatt
     2701 gatttaaaaa acactagact gtatctgctc agctttgttg gctacttaaa gtgggcagat
     2761 acgtcattag aagaaaaaac ataagatgtg accacttaca taaatttgtg ctaaactgag
     2821 gtagtacaag aataaattac atcataaagg agtcttttta atgagtaaag aaatgacagc
     2881 gctaaaattt tattttagaa atggagaaac ctggacgatc aaccgccgtc atatcggcga
     2941 tctttggatc aaacaaatca caacaagttt tggacgaatc aatggcagtg aatttgttga
     3001 aatccatccg tgtgctggat ttaaaatcga aatcttccat gaaggggacg cagttgcgac
     3061 acatgatatc aacctaggcg gactggaaat gggcatgttt aatcgcgcat tgaaatacga
     3121 agatatcgag cgaatggaaa tcctttaccg aaatggcaca cctgatcttg tgtacttccc
     3181 atatctagat aaaggaacag aaggattaga caatcaatat caatcaacaa aaatcagcga
     3241 aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa cgtgtagaag atgtatacgg
     3301 ggaatttttc gaataaatcg atttttacgc aaaaatgagg ctgtgaaaga gatgtgtctt
     3361 attactactg ataggctgtc tcttccacac cgtttattca gaataaggga ctgtgacaag
     3421 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
     3481 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
     3541 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
     3601 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
     3661 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
     3721 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
     3781 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
     3841 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
     3901 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
     3961 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
     4021 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
     4081 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
     4141 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
     4201 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
     4261 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
     4321 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
     4381 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     4441 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     4501 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     4561 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     4621 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     4681 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     4741 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     4801 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     4861 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     4921 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     4981 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     5041 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     5101 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     5161 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     5221 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     5281 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     5341 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     5401 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     5461 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     5521 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     5581 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     5641 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     5701 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     5761 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     5821 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     5881 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     5941 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     6001 ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc
     6061 aagttttata aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg
     6121 agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac
     6181 aatatccaag ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa
     6241 cttatggtga aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta
     6301 tcgggttgat tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta
     6361 tcgatagcag cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt
     6421 atctttctca agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag
     6481 gaaacagccc cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg
     6541 aaaacggaga agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata
     6601 aagaagatgc ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca
     6661 gtgaattaga taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg
     6721 cgcaagtgtt gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag
     6781 attatgaagc gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga
     6841 tggttaccca cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat
     6901 ttggaaaatt atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac
     6961 gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag
     7021 cttggatgag ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt
     7081 tcgaagatct aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc
     7141 tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa
     7201 atgaccaaac actgctagaa catttagatt tattgattca atctcgggaa agactaggta
     7261 ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc
     7321 ccctggatag cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag
     7381 aaaatgaaga aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag
     7441 aagcaaaaac agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat
     7501 ttccaagatt tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt
     7561 tatttttgtt gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca
     7621 atgatttgga catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg
     7681 ctgtgatcac tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag
     7741 tattccaagg aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga
     7801 agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac
     7861 ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg
     7921 agattgcaga acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag
     7981 ataatttcac tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg
     8041 aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag
     8101 gatggaacaa gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga
     8161 agacagaaca ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca
     8221 aaaagggttt cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt
     8281 gctttggttt ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat
     8341 ttgggatgaa tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat
     8401 gactgatttt ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca
     8461 gagcaagctt ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga
     8521 tcttggacac atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat
     8581 cgatcaaatc aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat
     8641 cgtttctgca tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat
     8701 gtttcaattt tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga
     8761 tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc
     8821 acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta
     8881 tatcaatcat atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac
     8941 tttagttttg aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt
     9001 agaaggatat gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg
     9061 atgagaaaat gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag
     9121 accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa
     9181 atacactagt gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc
     9241 gtcagacgat tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc
     9301 atagtgtgga agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg
     9361 gagggtcagc tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca
     9421 tccatcatgt attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac
     9481 tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa
     9541 cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa
     9601 gctatggata gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt
     9661 aagtaaagaa taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc
     9721 aaacaacatg catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag
     9781 aaagcccgcc tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa
     9841 cagcagccac gacacagagc caccccataa aaatatagat gatcgtagat acttttgata
     9901 ctctctcccg tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc
     9961 agattaatac aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag
    10021 tataacttcc tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt
    10081 ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca
    10141 tcgatccata gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta
    10201 ctaaaagaac cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat
    10261 taaacacttc atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg
    10321 ctcctttact aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa
    10381 attgtcttac atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa
    10441 ataatgacaa atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc
    10501 gatgctttag gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc
    10561 gatgatgacc aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg
    10621 cccaaaacaa gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa
    10681 gacggcagtg aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca
    10741 gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact
    10801 gaccaaggat tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca
    10861 gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga
    10921 atatccacac tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg
    10981 tctagtgttt tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta
    11041 actaaggggc gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg
    11101 aaagaattgc caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg
    11161 caagaattca aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat
    11221 acgaatccgg ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta
    11281 gattagtcga aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc
    11341 tttataaaat taggaaaata attgttttct tttttcataa agactacctt tttgataaaa
    11401 tttttgataa cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg
    11461 acatttgatc ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc
    11521 tgcttctata ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc
    11581 aaataaaaaa gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg
    11641 agatcaaacc aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta
    11701 taatttgacc gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat
    11761 cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat
    11821 cacactgact gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt
    11881 aacggtcaat tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt
    11941 ggaagaattt cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc
    12001 attttatttg aactttcctg agatcactga catgcagaca attgttaaca attggaacaa
    12061 aggaacagaa acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt
    12121 gctttatcaa ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac
    12181 tagcacgaca gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt
    12241 ccatccaaat aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga
    12301 gacacaaaac caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac
    12361 gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt
    12421 ggggtagtac ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca
    12481 gaactcccag aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat
    12541 tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat
    12601 agtgatattg aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa
    12661 gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga
    12721 aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat
    12781 gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat
    12841 gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa
    12901 gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat
    12961 acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc
    13021 aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga
    13081 acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc
    13141 tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga
    13201 acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac
    13261 attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc
    13321 tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga
    13381 agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga
    13441 acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca
    13501 tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc
    13561 ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg
    13621 acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa
    13681 gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg
    13741 aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa
    13801 aataatcatg acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca
    13861 atagcctaat tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt
    13921 ttcttataat aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa
    13981 acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct
    14041 tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga
    14101 aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt
    14161 tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct
    14221 tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa
    14281 ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga
    14341 ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact
    14401 tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga
    14461 tattctaggt aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg
    14521 agaaacaata aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca
    14581 gtttactacc ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa
    14641 gttttgataa atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg
    14701 aatctaccta gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa
    14761 ttgataaaaa ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg
    14821 tttatctttt tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata
    14881 agaaaagcag cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac
    14941 atttcaagca aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa
    15001 aatccgaatg tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca
    15061 agggctaata aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa
    15121 acgtcttttg caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg
    15181 ataccataaa atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa
    15241 caattaaaaa aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat
    15301 aaaaaaaata gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa
    15361 aaacgtcaga ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt
    15421 ctagatcttg gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt
    15481 ggaaagtacc accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt
    15541 caacactaaa cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt
    15601 ttataaattt tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata
    15661 ccattgaacc agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat
    15721 aaaaagcaac ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca
    15781 tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca
    15841 aaaatttaga ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga
    15901 tgtgatagaa tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat
    15961 aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta
    16021 tgaaaggaat ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc
    16081 gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac
    16141 gattaccttt atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc
    16201 ttgttttatt gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat
    16261 attttcgtaa acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta
    16321 aaggtctagt acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc
    16381 gggggatcaa gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat
    16441 ctacgttgat gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg
    16501 taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag
    16561 ggattttatg gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg
    16621 cgatcaaaga ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg
    16681 cccgatatta ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa
    16741 aattggcacc ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg
    16801 atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac
    16861 tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa
    16921 gaacgattga aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat
    16981 ccacgcaaag gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca
    17041 ttaattaagg aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt
    17101 caccaatgga ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg
    17161 gcagctgcag ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa
    17221 ttatcagaga aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc
    17281 gggatcggga tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa
    17341 ttagcgatga tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat
    17401 gcgatgacct tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct
    17461 gtgtcgattg ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa
    17521 tatgacgtgc tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa
    17581 cacttagaag gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct
    17641 gtaaaaaaat atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac
    17701 gaaaggcggt tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa
    17761 aggaatcagt agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa
    17821 agaaatcgga tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt
    17881 tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt
    17941 tatcacttgc tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat
    18001 tgtactgttt tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg
    18061 tggaagacac acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat
    18121 agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca
    18181 agcagcaatc tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt
    18241 ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag
    18301 tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat
    18361 cgcagctttg tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat
    18421 aacagcacag caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt
    18481 aacagaacat tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc
    18541 gggtgaagat atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt
    18601 gacaaacgtt ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag
    18661 taggaacacg acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg
    18721 tagaatcacc tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa
    18781 tggccagtgt tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa
    18841 acaattatga accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga
    18901 aagccgcagc aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag
    18961 aagcgattgc ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg
    19021 tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga
    19081 tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt
    19141 attcgatcag tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc
    19201 aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag
    19261 aagaatattg gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt
    19321 tctggggaat cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca
    19381 ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa
    19441 gaaaccctgc gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa
    19501 actttcgaac aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg
    19561 gtaaacaagg aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg
    19621 atacagcaaa agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta
    19681 gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc
    19741 gtccaacgag tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat
    19801 tgaaacttta tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt
    19861 tagatacaat gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa
    19921 aaatcaaatt caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag
    19981 agaaagaaaa tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg
    20041 aaccaaaaca acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg
    20101 cactagaaga aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc
    20161 aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa
    20221 ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag
    20281 aaatggaagg agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg
    20341 atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa
    20401 ttcaaatcaa agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga
    20461 taaaattagg gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata
    20521 cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca
    20581 ttgaacgaaa atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg
    20641 aattcacttc ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg
    20701 tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag
    20761 acatccaaaa ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca
    20821 gggcttgccg gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta
    20881 tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta
    20941 gtatgttcca attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa
    21001 gaaatgcgcc gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct
    21061 ggtggtgcct tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa
    21121 gagcatccat tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc
    21181 gtagtagcaa caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat
    21241 ggttcagatg gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc
    21301 gatatggata aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac
    21361 tgtccgatga cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg
    21421 gcagaactta aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta
    21481 atggcacagc gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa
    21541 gatcctaaaa ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca
    21601 gcttctttat acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt
    21661 gttttccgga tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac
    21721 cgaaacagct ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc
    21781 gaagatctgt tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg
    21841 agcggactac ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag
    21901 tttccacaag aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa
    21961 catttccaac cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga
    22021 gacaaaaaag caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata
    22081 aaaacagaat tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg
    22141 agacggatag tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg
    22201 aggaattgcg ccaattgtga acaaacttag aaatattggt taaattctga caatttattt
    22261 gtctttttat aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga
    22321 cagaagccac gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag
    22381 aaaaaacgaa agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat
    22441 ctggagacgc tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat
    22501 tagctgatca tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac
    22561 gttcgtttta tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg
    22621 ttcatctcaa aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag
    22681 cactttttga cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg
    22741 aagtccttta cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca
    22801 ttgattttga ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc
    22861 ctctaggttc ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat
    22921 tgatgacaaa atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa
    22981 tcacgcctac tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata
    23041 gtgatatcca tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg
    23101 cagatttgcg gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct
    23161 atgcccatgt aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt
    23221 aaaaataagg aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt
    23281 caaccactat ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag
    23341 taacaatggg tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca
    23401 atggagaagt tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa
    23461 aatttgaagg caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag
    23521 ctcaagaatg gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga
    23581 atgataaaga aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta
    23641 tcttagctat cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact
    23701 atgctcaaaa cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag
    23761 atatctgtat ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat
    23821 atgtatgaat taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta
    23881 ggacagcacg cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg
    23941 cagttagatg aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct
    24001 acaggggtcg gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt
    24061 gtcaaagtcg aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca
    24121 atggttcggg atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt
    24181 gtatacacaa gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt
    24241 atcaaaaaag aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat
    24301 gctagtcagc aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat
    24361 cgccgtacag tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt
    24421 gaagttgaag accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg
    24481 atcgacctaa atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta
    24541 acagtcaaag aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat
    24601 gctgatatcc atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat
    24661 gaaatcgata agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc
    24721 caaagagaca tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc
    24781 caaacagatc acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat
    24841 ctaattccag aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct
    24901 gaagattttg tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg
    24961 ttgatcggta cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct
    25021 caaatcgctt tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc
    25081 attttggaaa aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa
    25141 ataacgatca cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat
    25201 ttaagtcgct atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa
    25261 cccgcaagat aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc
    25321 cttacgataa aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag
    25381 gaagtgacgg agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca
    25441 aaacaatgtt tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga
    25501 tgaaaactga agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa
    25561 gagagaaata tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt
    25621 tgggtacgat tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag
    25681 cagaatatgg agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg
    25741 aagcagatgt acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg
    25801 aattgaaggc tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg
    25861 cttctaatat tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa
    25921 aattggaatc agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa
    25981 aagtattgaa ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg
    26041 actggggcag atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg
    26101 gaggaatggc aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac
    26161 acggtgcgga attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag
    26221 gtgatccagc gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa
    26281 aagatgatca gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc
    26341 gggatatgga ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga
    26401 gtacgccgga gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat
    26461 taggaggaga cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt
    26521 tcttttctat cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg
    26581 aagatttcta tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc
    26641 cattcatcga tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc
    26701 gtgaactttt tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca
    26761 ttcgaagtga aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag
    26821 ggatctggtc tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa
    26881 tcgctgacac ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag
    26941 gtgcccatga aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg
    27001 agaagttagc agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata
    27061 agggagtgtg acatcgattt gtcacactcc cgttttatct ctttggaatg aaaaagtatc
    27121 aggatttttt cttgctgcta ttcaaaccaa acgggattcg actttcagta tggtttctga
    27181 tacgcttgat attgtcttta tgccgataaa agataaagga agaaactaaa aaggcaatga
    27241 tcgtcagtaa ccaattgggc ttagccaaaa tcacaggagc tacataagga aggacgatcg
    27301 tagataaagt gatcaatact gcactgatca tactgctcaa actcaccata cttgtacaaa
    27361 ataaactaac gataaagatg atacaagaat aaacaaaaaa tacaggatta aatcctaaaa
    27421 gcattcctgc acttgtcgca acagcttttc ctcctttgaa cccagcaaaa atcggaaatg
    27481 tatggccaag aatggcgcat accccaaacc aaagagggtt aatcccagaa aggtggaaga
    27541 tcatcggcaa acaagtagcc aatgtacctt ttaaaatatc cataagcagg acaacaatcc
    27601 cggctgtttt gcctaataca cgaaatgtat tggtcgttcc agtattaccg ctcccgtgtt
    27661 gtcggatatc ttttttgaaa aacaatttgc caatccagac accggaagga atagaaccta
    27721 aaagatatgc taaaataagt aataagatca atttcatttt ttaccctctt tcaagttgaa
    27781 aactatcctt tattttagca tgaataggta acaggaacaa ttgaaaaaat gaaagataca
    27841 agtataatag atggaataaa aagaaaagca aggggtggca gaaatgtctg taaaaaatcc
    27901 aacacaagaa acgattttca actatttgga agaagcaaaa aatattgccg tcgtaggctt
    27961 gagcaataaa ccagaacgaa caagttacat ggttgcacag gtattgcaag acaatggata
    28021 taaaatcata ccagtaaatc cattattggc tggtgaagaa attctaggcg agaaagtcta
    28081 tgcgaatttg acagaaatcg atgatccaat cgatatcgta gatgtattta gacggagtga
    28141 atttttacct gaaatagcag gtgatttttt gaaaactgat gctaaagtgt tctgggcaca
    28201 attagggatt gaaaacaaag aagcagctga gatgctaaga aaagcaggac gtacagatat
    28261 cgtaatggat cgttgtatta agattgaact tggtaagttg aaagaaaacc aaaaataaaa
    28321 atgaccacaa agaagagatg atcgatagag gggaatgtcg aaaaagcggt taggagataa
    28381 ttaaaattga tttctcgtct accctctttt ttttcacgac cttttctagt atgataatcg
    28441 tggtgcggtt ttgccgcaaa gtattattga aggagcgttt gccttggcaa aaaaagtaaa
    28501 caacgaatac aatgactcgt ccatccaagt attggaagga ttagaggcag ttagaaaaag
    28561 accagggatg tacattggat ccactgacag ccgaggcttg catcatttag tctatgaaat
    28621 cgtcgataat gcagttgacg aagcattatc aggttatggc agtgagattg atgtgaccat
    28681 ccatgaagac aatagtataa ctgttgcaga tagcggacgt gggatgcctg taggaatgca
    28741 tgcttccgga ataccaacag ttgaagtgat ttttactgtg ctgcatgctg gcggaaagtt
    28801 tggtcaagga gggtacaaaa cttctggtgg actgcacggt gtaggagcca gtgtggtaaa
    28861 tgccttatcg aaatggctaa ttgtaacgat cgttagagat ggcgtagagt atcagcagaa
    28921 atttaaaaac ggcggtaaac ctgacggtac tttgaaaaag ataggaaaaa caaaaaaagc
    28981 aaacgggacg actgtccatt ttttaccaga tgatacaata ttcagtacaa cgaaattttc
    29041 atatgaaatt ttagccgaac ggttaagaga atctgccttt cttctaaaag gcgttaaaat
    29101 ctctctttct gatcttcgtg gagaagaacc agtcaaggaa atttttcatt acgaagaagg
    29161 aatcaaagaa tttgttgatt atctgaatga agaaaaagat acattgacac cagttgttta
    29221 tttttcagga gaaaaagaag gtatcgaagt agaggtcgct tatcaataca atgacggtta
    29281 ttcagaaaat gttttatctt ttgtcaataa cgttcggaca aaagatgggg gaacccatga
    29341 agctggtatg aaagcggcga tgacgaaatc ttataatgaa tatgcgagaa aagttggttt
    29401 gctgaaagaa cgggacaaga atctggaagg tagtgatttc agagaagggt tagctgctgt
    29461 actttctatc cgtgtacctg aaaatcttct gcaatttgaa gggcaaacaa aagaaaaact
    29521 tggtacgcca gttgctcgta ccgtagtaga taacgtcatc agtgaacaaa tgggctttta
    29581 tttgcaggaa aatagcgaaa tgagccagat gttggtccgt aaagcaatca aagccagaga
    29641 agcgagagaa gcagcacgta aagccagaga agaaagccga aatgggaaga agcgtaaaaa
    29701 aggcgaatct cttctttcag gaaaattgac acccgcacaa tcccgaaacc ctaagaaaaa
    29761 tgaattgtat ttagtcgaag gggattctgc tggcggatct gctaaacaag gaagagacag
    29821 aaagttccaa gcaattctac ctttacgagg aaaagtcatc aataccgaaa aagctaaaat
    29881 gcaagatatc ttgaaaaatg aagaaatcaa tacgatgatc tatacgattg gtgcaggagt
    29941 cggtcctgag ttttctatcg aagactgtaa ttacgacaaa gtaatcatca tgaccgatgc
    30001 ggatacagac ggcgcccata ttcaagtatt actgcttact ttcttttatc gttatatgaa
    30061 accgctgatt gaagctggga aagtatatat cgcgttaccg cctttatata aagtatccaa
    30121 aggacaagga aagaagcaag tcattgaata tgcgtggaca gatgacgaat tggcagcaat
    30181 gattaaaaaa gtcggcaaag gatatatgct gcagcgatat aaaggacttg gagagatgaa
    30241 tgcggaacaa ttgtgggaaa caacgatgga tccgacttcc cgtacgttga tccgcgtccg
    30301 tatcgatgat gctgcgcaag ctgaacgtcg agtaaccaca ctgatgggtg acaaagtaga
    30361 acctcgaaga aaatggatcg aaaatcacgt gcaatttacg ctcgaagaag atggcagtat
    30421 cttagataaa aaagaggata ctgaaatctc tccttcagtt tctaatgatt tattagatga
    30481 agaacgagca gataaaaatg aaaacaatca gttatttgaa gtagaatagg aggtcacctg
    30541 atggaaaatc gacaagaagt acaagaatta actttagaag aagtgatggg tgaccgcttc
    30601 ggacgttatt ccaaatatat tatccaagaa cgtgcactgc ctgatatccg tgatggattg
    30661 aagccagtgc aacgccggat attatttgcc atgaacaaag atggcaatac gtatgacaaa
    30721 ggattccgta aatcagcaaa atctgtcgga aatatcatgg ggaattatca tcctcatggt
    30781 gacatcagta tttatgaagc gatggtccgc atgagccaag actggaagtt gcgggaagta
    30841 ctgatcgaaa tgcacgggaa taacgggagt atggacggtg atccacctgc tgcgatgcgt
    30901 tatactgaag ccagattatc caagttaagt ggagagatgc tagcagatat cgagaaagaa
    30961 acagtcgatc tcgtgtggaa ttttgatgat acagaaaaag aaccaacagt ccttccagct
    31021 cgatacccaa accttctcgt caatggatcg acaggtatat ctgctggata tgcaacagaa
    31081 atcccgactc ataacttagc tgaagtgatc gatggcacaa tctatatgat cgatcatcca
    31141 caagcgagcc ttgaaaaatt gatggaatat attcctggac ctgactttcc aacaggaggg
    31201 atcttgcaag gaaaagatga aatcaaaaaa gcttatgaaa caggacgagg aaaagttatt
    31261 cttcgttcga aaacaaagat agaggcaatg aagggtaata aacagcaaat cgtgatcaca
    31321 gaaatcccat atgaagtaaa taaagcgacc ttggtgaaaa aaatggatga gatccgtttg
    31381 aataagaaaa tcgatggaat agctgaagta cgagatgaaa gtgatagaac aggtctgcaa
    31441 attgtcgtcg aattgaaaaa agacgccaat gctcaaggaa ttttgaatta tttgttcaaa
    31501 aacactgagc tacagatcaa ttacaatttc aatatggtcg ctatcgacca tatgactccg
    31561 catcaagttg gactgaaaga cattttaaga agttatatcg agcatcgaaa gcaagtcata
    31621 acaaaacgta gtcaattcga tttagccaaa gctcaaaaaa gacagcatat cgtagaagga
    31681 ttgatgaaag ctttatcgat tctggatgaa gtaatcgcaa cgattcgtga aagtaaagac
    31741 aaaaaagacg ccaaaaagaa tttagtcgac gtctttcaat ttacagaaga acaagcagaa
    31801 gcaattgtca cattgcaatt ataccgtttg acgaacacgg atatcacaga gcttcaaaag
    31861 gaatccgaaa gcttgattgc gcaaatcaca gagttgaata aaatcctttc aaatgacaaa
    31921 gagcttttct ctgttatgaa aaaagaattg cgagaagtga aaaaaaatta ttcttccgct
    31981 cgtctaacga caatcgaaga cgaaatcgaa gaaatcaaaa ttgatacgca agtcttagtg
    32041 gcacaagaag acgtaattgt cagtgtgacg cgagaaggct atgtgaagcg aacaagcctt
    32101 cgttcttata gtgcttccaa accagaagaa atcgggatgc gagaaggcga ctacttgcta
    32161 tactcaggtg aattgagtac attggaccat gttcttctga tcacaaataa agcaaatgtg
    32221 atctaccgcc ctgtccatga actacccgat ttgaaatgga aagatgctgg tgagcacatt
    32281 tctcaaacaa tctccaatct gtctgtagac gaatcgattt tagctgtttt cccatatcaa
    32341 aaaattcaag cagagaaaac atttgtattc atcagcaaga atggattgat caagcaaaca
    32401 cgaatgacag agttcgaacc atggcgcacc tataagagcc gtccgctcag ctgtatgaag
    32461 ctaaaggcgg aagaagatga actgattgct gtgtatctga aagaagatca gccaggctta
    32521 gatgttttct tagtaacgca tcaaggaatg ggtttgcgct atcctttaac agatgttccc
    32581 gttgttggag caaaggcagc tggagtgaag tctatgaatt tgaaagatgg agataggatt
    32641 atcaacgggc tgcttgtgtt agcagaaggt gatacgccga tcgtaatcgt tactcagcga
    32701 ggagctgtta aacgcatgct tgctcaagaa atcagccaaa cttcacgtgc gaagcgggga
    32761 gtgacggtca tgcgtgagct gaaaaaaaat ccgcaccgta ttatctatat gtcggaagga
    32821 agatcaaaag aaataacctt gatgaatcaa aaagggcagc agttaacgat tgacccaact
    32881 gatttcccta taggtgatcg aacatcgaat ggatcatttg cgatggatga gaaaaaaggt
    32941 ggagaagtca tcgaagttat cgatgctcct gagattttca tcgagtcgta actgttgtag
    33001 tattttaatg aagagacgcc atctgtatta atacaggtgg cgtctctttt tcattcattt
    33061 ttaataaatc cttatacgtt tgcaaaaaaa gatgaaagtg ttcacaatgc ttaaatgttg
    33121 gtatgtcaac gttgatttaa atatatgtaa ataaaacata aaaattgtta aataaaaaac
    33181 aagaaaaccc ttgcaaagat agggtgctgt gttatactaa tcacgtaaaa actgttacat
    33241 aaaacagaaa tttaggaggt ccatacaatg gagcaatgga atggatttaa aggtaccaaa
    33301 tggaaagaag gcgtagacac tagagacttt attcaaaata actacactga atatcgcggt
    33361 gatgacagct ttttggaagg cccaacagaa gcaacagaaa aattatggga tgaattgcaa
    33421 aagttaaatg atatccaatt cgagcgtaac ggtgtctatg atatggacac agaaatcgta
    33481 tcaactatta cttcacacga tgcaggctat ttggataaag atttggaaaa aatcgtcggt
    33541 ctacagacag acaaaccttt gaaacaagca ttcatgccgt tcggcggaat caatatggct
    33601 aacaatgcgc taaaaagcaa tggctataca ccaaacgatg aattgacaca tattttcact
    33661 gactggcgca aaacacataa tcaaggtgta tttgacgctt atactgctga aatgcgtgct
    33721 gctcgtaaaa acaaaatcat tacagggctt ccagacgctt acggtcgcgg acgtatcatc
    33781 ggtgactatc gtcgtgtggc attgtatgga atcgactatt tgatggaaca aaagaaaaaa
    33841 gatcatgata tgactggtta tcatacaatg agtgaagaag tgattcgttt acgtgaagaa
    33901 atcactgaac aatatcgtgc attagcgcaa atgaaagaaa tggctgcgaa atatggctat
    33961 gatatttcta aaccagctgc aaatgcaaaa gaagctgtcc aatggctata cttcggatat
    34021 ttagcagcaa tcaaatcaca aaatggtgct gcgatgtcaa tcggacgtat ctcagcattc
    34081 cttgatatct atatccaacg tgatttggat aatggagtaa tcacagaaaa agaagcacaa
    34141 gaattgatcg accacttagt aatgaaatta cgtatggtca aattcgcgcg tacacctgaa
    34201 tataaccaat tattctcagg attccctatc tgggctactt tatccattgc aggtatggga
    34261 ttagatggtc gttcattagt tacaaagaat gatttccgtc tgcttcatac attaacaaat
    34321 atgggacctt caccagaacc aaacttgact gttttgtatt ctgaacacct tccagaagga
    34381 ttcagaacat atgctagcag aatcgctatc gaaagttctt cgattcaatt cgaaaatgat
    34441 aatcagcttc gtgaacaatg gggttctgat gattgtgcga ttgcttgctg tgtatcagga
    34501 acagttgtcg gaaaagacat gcaattcttt ggcgctcgtg ctaacttggc taaaacacta
    34561 ttatatgcca tcaatggcgg agtcgatgaa gtgactggcg cccaagttgg accagaatat
    34621 cgtccaatca catcagaaat actagacttt gaagaagtga aacataactt catgaatatg
    34681 atggattggt tagcagaact atatgtcaat acgttgaaca tcattcatta tatgcatgat
    34741 aaatattctt atgaagcagc tcagctggct ttgatggatt ctgacttgca acgcacattt
    34801 gctacaggta ttgctgggat ctctcatgcg gcagacagct tgtcagcgat caaatatgca
    34861 aaagtaaaag caatccgtaa tgaaaaagga atagcagttg attttgaagt ggatggcgac
    34921 ttccctaaat acggaaatga tgatgaccgt gcagatgaat tagtttcatg gatcttggaa
    34981 tacttcatgg gacaaatcaa acgtcataaa acttaccgca actcaaaacc aacaacttca
    35041 ttattgacga ttacttcaaa tgttgtttac ggtaaaaata caggaaatac tcctgatggc
    35101 cgtgaagctg gaaaaccact tgcgccagga gcaaatccaa gttacaatgc ggaacaaagt
    35161 gggttattag ctagcctgaa ctcaacagct aaaatgaatt acggctgtgc attggatgga
    35221 atttcaaata cccaaacaat caatccttca ggtcttggaa aagaacaaga tacaagaatc
    35281 gaaaacttgc gtaatgtctt agatggatac ttctcaaaag gagcttatca tttgaatgtc
    35341 aatgtattca gtaatgattt attgcgtgat gcaatggaaa atccaatgaa atatccgaac
    35401 ttgacgatcc gggtatctgg ttatgcagtt aaattccgtg acttgacaag agaacaacaa
    35461 ttagatgtct tgatgagaac ttcacacgac agaatgtaaa aagttcgtga aaaaagtttt
    35521 tcgacttagc gtcgaaagga cgttgaatag ccatcgcatc aaaatcgaat aatcagtatg
    35581 cttatttgaa agaaacgatt tacaggggca ggacttgtat gttccgtccc tgtaaatttt
    35641 acacataacg aagcggcaat tgcgaaaaat tttgttcaga aagcttcgct tcgacagcta
    35701 acttgtactt aaaatgaaat caaagaaaag atgtgaactt aaatggaaga aaaaacaatc
    35761 ggctatgttc attctatcga aactttcggt tcagtagacg gacctggact gcgctttgtc
    35821 gtgtttatgc aaggatgccg catgcgttgc caattttgtc acaatccaga tacttggaat
    35881 attggcggag ggaaagaata cacagcagat gaacttttag ataaagcaga acgcttcaga
    35941 ccttactggg gagataaagg tggtattact gttagtggtg gagaacctct attgcagatc
    36001 gattttttga tcgaactatt taaaaaagca aaagagcgga agatgcatac tacattggat
    36061 acatgcggaa aaccttttac gtatgaagaa cctttctttt cacgttttca agaattgatg
    36121 aagtatacag atttactgtt atttgacatc aaacacatcg ataatgaaga gcataaaaaa
    36181 ctaacgcatt gggacaatga aaatatttta gaaatggctc aatatttgtc aaaaatcaac
    36241 aaaccggttt ggattcgcca tgtactagtt cctgagcgaa gtgattatga tgaatatttg
    36301 atccgactag acaactttat tcaaaccttg tcaaatgtag acagagtaga gattcttcct
    36361 tatcatacga tgggaaaata caagtgggaa acacttggtt tgaaatatcc attagaaggg
    36421 atcgaaccac ctacaaaaga acgtgtcgaa aatgctaaac gattgcttca tacagaagag
    36481 tatacaaaat atttaacgcg ataggtcgca tcggaaagaa gataagtatt gcttctttcc
    36541 gtttttttgc tattattaaa taggagaaat cacaaaggag cgaatgacat gtcaaaaatt
    36601 ttagtttttg gtcaccaaaa tccagataca gatgcaatcg gggcagccat tgcgttttca
    36661 cacttgcaaa atgaattagg taaagaaaca gaagcagttg ctctaggtac acctaatgaa
    36721 gaaacacaat acgcattaga ccactttcag ctatctgcac cacgagtagt agagtcagct
    36781 aaagcagaaa atgcagatgt catgcttgtg gatcataatg aattccaaca aagtatttca
    36841 gatatcagcg aagtaaatat tttagctgta gtagaccacc atcgtattgc aaactttgaa
    36901 acagctaatc cattgtacta tcgtgcagaa ccagttggat gtacaagtac gattatttta
    36961 aaactatata aagaaaacca agtaacaatt cctaaagaaa tcgcaggaat catgctctca
    37021 gctatcattt cagatacatt gttgttcaaa tcaccaacat gtacgcctga agatgaagca
    37081 gctgcaaaag aacttgcagc aatcgctgaa gtcgatctaa acacttatgg acttgaaatg
    37141 ttgaaagcag gaacaaatct aagtgataaa tcagctgcgg ttcttcttga tttggatgca
    37201 aaaagtttcc caatgggcag ttccaacgtt cgtatcgctc aagtcaatac agtggacctt
    37261 aatgaagtga tggatcgaca agcagagcta gaagcagcaa tggaagatga aatgaaagca
    37321 aatggctacg atctgttcgt attagttgtc acaaacatct tagacagtga ttcagaatta
    37381 cttgttgctg gtaaaggaaa agaaaaagta gaagaagctt tccaaacaac tttgaccaac
    37441 aaccgtgctt tgcttaaagg tgtcgtttca cgtaaaaaac aagtcgtacc tcaattgacg
    37501 gctgtattta gctaatcaaa tggtagtatg aagcattcaa aatgaatgga taagcgattg
    37561 atataaaaag aaacatagaa catactaaag gccgaagaaa tggaggagaa taacagtcaa
    37621 ttatgactct tgttcttcct cctttttacg taaaagcctt ttaagtaaaa taaaacggat
    37681 agaaaagaga acatatatga actattattc aaaaaataag gaaaactttt atcaaaaact
    37741 gaccgatgat ccgctttttt cttcattgac cgattatcta tatgaacatc gagaacaaga
    37801 aacgattcta agagaattga aaaaagaatt ttcgcaaaat aaattttccc attttcttga
    37861 tttattgatc gatgcaggat taatcaagag ggaagaacga agatatcatt tgaactttcc
    37921 aatatttgat cctaaagatt acttgcaaca agccacttcg gctgctgaaa ccatagctga
    37981 acaattgaag agactctctg ttgatgaaca aaaattagcg atgggagaag ttatttgggc
    38041 gtattgtttc gaagatgaac ggaaagaagc gtatttttat ggagtaagaa acagccgaga
    38101 gacagaattg ctgaggacaa ctgcaggaaa tgagaagtac cgttttatca cactttcctc
    38161 tatagaacac ttcccgctta ctttggctaa ttactttttt gtacagaaga atcagcttcc
    38221 tgtaacgaag gcattcaaag aattagccga acttattgga gacgtaaatg aatcgtactt
    38281 ttttgatcaa atagaagtta tcgttgacag gatacgtaag aataaatata aaaatcgccg
    38341 gcctagtatt ttccatcagt ctctacttgt gacgaatact ataaaggaag aagaatcatt
    38401 cacattagaa ttacctattg tcgaaaaaaa caattttgag attgaattac caacattgga
    38461 tccttcattg acaatggagg aaatggcctt tcttaaacga caaatctttt ctgagctgag
    38521 caagaaattt ataccgcatg cattttctta tatcaaagaa tatggaacag ttttagtgag
    38581 taaaacctaa gagagcagta agcaaaaatt atactgactc ttgcaggagg agcctttctc
    38641 ttcctttatg gttaaaagaa ttatttgagt attttgccta tctaccacct attagattca
    38701 ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg
    38761 aagtagtgat aagaaaagtt agatttttaa ataatcaata ttatgattca gtcaagtatg
    38821 gcattttatg ggaagaacta gctgattaga ttaggaagtc catttgataa tccagaatca
    38881 ctaagaaacg aatggcgagt cattaataat ttatccaatc ttgggttatt cttttagatt
    38941 gaagtgttta aaactaaaaa agcatttatg aagtcgtaat agtcaggctt caaaatgctt
    39001 tttattttgt ttgttttttc tttgccacat aaatacacct agactgacga tgaagagcat
    39061 atccaacaga tttcctgcga cttggatcgt cgaagggttg gtcagtggca ttaaagcaag
    39121 gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc gaaagaagag
    39181 cggacatttc aattggctaa aaatgagtga aaaaataaaa taagtaaaaa tggataaaat
    39241 cacgactaaa ccattcatga taaagaagaa aagcgtattg tgattaaacg aataaaaaag
    39301 aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt
    39361 accaggatgt cgatgaaaaa aataggcacc gaaataaaca gaaaacagta agccgcaaag
    39421 agaagacaaa atcaaaaaaa acggattttc ttcggcttct gtgatatcag aatgatccaa
    39481 aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg aataacatag
    39541 aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct
    39601 acgttgaatt tccataatat ctccaaatta aattgataag tatataaagt ataataacaa
    39661 attgatgagt atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt
    39721 taaaatttaa tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt
    39781 tctcactttt ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa
    39841 agcacgctct tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga
    39901 aagtatttcg agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc
    39961 ataaccgtgt tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa
    40021 aatcgttgtg accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact
    40081 gatacctact gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc
    40141 taaaacaagg gataagccga cgatataaat cgtgatctcg accatactga caagtggagc
    40201 aatcagtcga tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc
    40261 aaaattaatg aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt
    40321 catttatata acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac
    40381 atacaaaaaa atgataaaat gattataata gaattataaa gataggttag gtgattaaaa
    40441 tgaaaacggt caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat
    40501 acttaggtgt ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt
    40561 cgctgacaga tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata
    40621 ttttcggtag cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat
    40681 taggagtggt gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta
    40741 tattatttgg tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat
    40801 caggaaattt aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg
    40861 tccatcatat tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg
    40921 ttgtcatcta ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa
    40981 aaattgcgca gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca
    41041 aacagccggt ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt
    41101 ttgaatttga cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg
    41161 cagttggcgg ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc
    41221 atatagtagc gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa
    41281 tcgtaaggcg cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt
    41341 catgggttaa gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt
    41401 tcttttggca tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag
    41461 aaaaaaatcg gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc
    41521 gacatatggt acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga
    41581 taaaaaagta taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt
    41641 tttattttgg gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg
    41701 acaacatgtt agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa
    41761 tgtttcaaaa atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt
    41821 gaaagagtta gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa
    41881 agcacttgtc acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac
    41941 aactgagccc tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca
    42001 ttattctctc caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat
    42061 catcacaggt gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt
    42121 cttgtttggg gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac
    42181 agcaaaagca acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat
    42241 cgaagtggac gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga
    42301 agttgatctt cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata
    42361 tatgacagaa aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg
    42421 gctagcacta ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt
    42481 tggtgtgatt ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac
    42541 gatcaagcaa gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat
    42601 agaagaaaaa ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga
    42661 gacaacgaat taagtcttat tactatatat atatttatga aattcatcgc tattttctta
    42721 aaataatcag cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc
    42781 atttttgaga aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc
    42841 gtacgatgat gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg
    42901 acaagaagat cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca
    42961 tttcacgaac acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa
    43021 gtcagctccg cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg
    43081 ccattaccaa tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg
    43141 ttctgtcaga caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt
    43201 tgacacgaag gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat
    43261 cccagaccct agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac
    43321 ggatgactct tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg
    43381 tgagccgtct gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga
    43441 agaaaaaaaa tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga
    43501 tgatcaagga aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat
    43561 attcggctac agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa
    43621 agtttatatc gtatcctcaa atttttttga gtttaagcga attttttctc cc
//
LOCUS       DAZUMT010000011        43054 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000011 DAZUMT010000000
VERSION     DAZUMT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.011"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..3396
                     /locus_tag="ACG8FM_000543"
     CDS             118..3396
                     /locus_tag="ACG8FM_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS5093233.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3937..5844)
                     /locus_tag="ACG8FM_000544"
     CDS             complement(3937..5844)
                     /locus_tag="ACG8FM_000544"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS5093234.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5974..6849
                     /locus_tag="ACG8FM_000545"
     CDS             5974..6849
                     /locus_tag="ACG8FM_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS5093235.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6929..7903
                     /locus_tag="ACG8FM_000546"
     CDS             6929..7903
                     /locus_tag="ACG8FM_000546"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5093236.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8083..9228
                     /locus_tag="ACG8FM_000547"
     CDS             8083..9228
                     /locus_tag="ACG8FM_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5093237.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9365..9709
                     /locus_tag="ACG8FM_000548"
     CDS             9365..9709
                     /locus_tag="ACG8FM_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS5093238.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9980..11266
                     /gene="efmA"
                     /locus_tag="ACG8FM_000549"
     CDS             9980..11266
                     /gene="efmA"
                     /locus_tag="ACG8FM_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS5093239.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11404..12003)
                     /locus_tag="ACG8FM_000550"
     CDS             complement(11404..12003)
                     /locus_tag="ACG8FM_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5093240.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12407..13528
                     /gene="mnmA"
                     /locus_tag="ACG8FM_000551"
     CDS             12407..13528
                     /gene="mnmA"
                     /locus_tag="ACG8FM_000551"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS5093241.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13774..15195
                     /gene="arcD"
                     /locus_tag="ACG8FM_000552"
     CDS             13774..15195
                     /gene="arcD"
                     /locus_tag="ACG8FM_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS5093242.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15209..16327
                     /locus_tag="ACG8FM_000553"
     CDS             15209..16327
                     /locus_tag="ACG8FM_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS5093243.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16387..17679)
                     /locus_tag="ACG8FM_000554"
     CDS             complement(16387..17679)
                     /locus_tag="ACG8FM_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5093244.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17829..18389
                     /locus_tag="ACG8FM_000555"
     CDS             17829..18389
                     /locus_tag="ACG8FM_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5093245.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18518..18895
                     /locus_tag="ACG8FM_000556"
     CDS             18518..18895
                     /locus_tag="ACG8FM_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS5093246.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18828..20900
                     /locus_tag="ACG8FM_000557"
     CDS             18828..20900
                     /locus_tag="ACG8FM_000557"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5093247.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21111..22058)
                     /locus_tag="ACG8FM_000558"
     CDS             complement(21111..22058)
                     /locus_tag="ACG8FM_000558"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5093248.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22304..22768
                     /locus_tag="ACG8FM_000559"
     CDS             22304..22768
                     /locus_tag="ACG8FM_000559"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS5093249.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22830..23873)
                     /locus_tag="ACG8FM_000560"
     CDS             complement(22830..23873)
                     /locus_tag="ACG8FM_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS5093250.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24250..25215)
                     /locus_tag="ACG8FM_000561"
     CDS             complement(24250..25215)
                     /locus_tag="ACG8FM_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS5093251.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25449..26294
                     /locus_tag="ACG8FM_000562"
     CDS             25449..26294
                     /locus_tag="ACG8FM_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5093252.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26485..27672
                     /locus_tag="ACG8FM_000563"
     CDS             26485..27672
                     /locus_tag="ACG8FM_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5093253.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27678..28034
                     /locus_tag="ACG8FM_000564"
     CDS             27678..28034
                     /locus_tag="ACG8FM_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS5093254.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28036..28374
                     /locus_tag="ACG8FM_000565"
     CDS             28036..28374
                     /locus_tag="ACG8FM_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS5093255.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28450..28706
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28769..29803
                     /locus_tag="ACG8FM_000566"
     CDS             28769..29803
                     /locus_tag="ACG8FM_000566"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5093256.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29796..30482
                     /locus_tag="ACG8FM_000567"
     CDS             29796..30482
                     /locus_tag="ACG8FM_000567"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5093257.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30506..31354
                     /locus_tag="ACG8FM_000568"
     CDS             30506..31354
                     /locus_tag="ACG8FM_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5093258.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31551..32324
                     /gene="sufC"
                     /locus_tag="ACG8FM_000569"
     CDS             31551..32324
                     /gene="sufC"
                     /locus_tag="ACG8FM_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS5093259.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32337..33623
                     /gene="sufD"
                     /locus_tag="ACG8FM_000570"
     CDS             32337..33623
                     /gene="sufD"
                     /locus_tag="ACG8FM_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS5093260.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33620..34855
                     /locus_tag="ACG8FM_000571"
     CDS             33620..34855
                     /locus_tag="ACG8FM_000571"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS5093261.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34842..35312
                     /gene="sufU"
                     /locus_tag="ACG8FM_000572"
     CDS             34842..35312
                     /gene="sufU"
                     /locus_tag="ACG8FM_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS5093262.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35317..36708
                     /gene="sufB"
                     /locus_tag="ACG8FM_000573"
     CDS             35317..36708
                     /gene="sufB"
                     /locus_tag="ACG8FM_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS5093263.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36803..37945)
                     /locus_tag="ACG8FM_000574"
     CDS             complement(36803..37945)
                     /locus_tag="ACG8FM_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5093264.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37984..38718)
                     /locus_tag="ACG8FM_000575"
     CDS             complement(37984..38718)
                     /locus_tag="ACG8FM_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5093265.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38950..39225
                     /locus_tag="ACG8FM_000576"
     CDS             38950..39225
                     /locus_tag="ACG8FM_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093266.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            39323..41320
                     /locus_tag="ACG8FM_000577"
     CDS             39323..41320
                     /locus_tag="ACG8FM_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093267.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(41396..41731)
                     /locus_tag="ACG8FM_000578"
     CDS             complement(41396..41731)
                     /locus_tag="ACG8FM_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5093268.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            41859..42073
                     /locus_tag="ACG8FM_000579"
                     /pseudo
     CDS             41859..42073
                     /locus_tag="ACG8FM_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            42110..42472
                     /locus_tag="ACG8FM_000580"
     CDS             42110..42472
                     /locus_tag="ACG8FM_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS5093269.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            42518..42844
                     /locus_tag="ACG8FM_000581"
     CDS             42518..42844
                     /locus_tag="ACG8FM_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093270.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        14116 a         7684 c         9022 g        12232 t
ORIGIN      
        1 tatatttttt ctttttataa aatacactta ttataatttt gttttttatt gacagtaaaa
       61 ataaattagg taatacttta agtaaacgct ttctaatttg ttacgggagg aatacacttg
      121 ttaaaaaaac gtataaaata cagtgcgcta atgatgaatc tccttatgct cagtaccccg
      181 gttttaattt ctgtcgttga agttgctgct aacgaacaac agtctgtgaa cgaagaaaat
      241 gcacacgtga acattgaagg gatgtcaaca atggaggaaa taccaagaac tagtgaagat
      301 atggatacgg aagaaggaaa tgatcctatg atgaaagaat cagaaaatga aaagacaaaa
      361 aagcaatctg aagaagcaaa tgaagagtgg aaaggggttg ttttcggaga aagcacaagt
      421 gctagtaggt atgccattga accattagaa gacggcgtgc ggctctcttc cacgaataac
      481 ggcggaaaat tccagtcaag cggatcagat ggaatgacct attattatca agcaatcccc
      541 aaagacatca actttaggat gcgggcgacg atcgaaatag aatcttggac ctatacgaat
      601 gcccaagaag gatttgcttt aatggtaaga gacgcagtac caaaaaatca tttgtttggg
      661 caggcatttt attcaaattc atttgccata ctaggcagtc ggatcgaata tgtctgggac
      721 tcggatagac aagaagtggt caacacgagc ggcagcaaat atacgatgcg acttggattg
      781 gggacacggg caatcacagg aatatcatct gaagatccgc aagctgcgcc agctcctggg
      841 agtgtctcag tggaaacaac acctttagag acttcagcag ggttcttgga aaaagaaaca
      901 ggttcttata atatattcgg aaacggtcat gggaatctgt ttccggccac gaactcgatt
      961 actaaactgg acgtagagat gaaaaaaacg aatactggtt tagaggcata ttattacgat
     1021 cctgaaacag gagaagaaat ggcttctgac atcatgtatg actgggaaaa attatacgct
     1081 tcagatgaat cggttattta tgcaggattt gctgcagcaa ggaatatgac gatcaaggta
     1141 gaaggaatcg aagttgctta ctcagatcct gcgacagatc ctcctgggca agaaagacct
     1201 actcgcttga ttgatccgat ttacactgtg acctctagta atcattcagg aaaccaagat
     1261 catactatgt cctttagagc aaatgctgac ggattattga caattaaaaa taaagcaaca
     1321 ggagaggtgc ttaagaacgc aaaaaacttg gctatcactg cgaatcggga atttgattat
     1381 cagacaaaac tgatggaagg aacgaacgaa tttactttca gttttacgcc agataaaaac
     1441 tatccgccag aagaatacgt agagatgact agttatgaaa caaaagagat tcttcataca
     1501 gtggactacc gtaaacctaa atattcgaca gtttatgtaa caccagaagg gtcagatgca
     1561 ggaaacggaa gcagacaaaa tccattatct ctgacacaag cattgagata cgcatatgct
     1621 gggcagacaa tcgtcatggc gcctggaact tattccttcg aaagaggatt gacgattcca
     1681 aaaggcgtta acgggacaag agaaaacccg attcagttga ttgcagagaa aaatccagaa
     1741 aacaagcgtc ctgtacttga ttttaaagga actggaaatg ggatgacact gttcagtcac
     1801 tactggggtt tacgagggtt tgacattaca aatagtgcag ctatgcaaaa aggattacag
     1861 atttctggaa atcataattt gatcgaagag attcatgctt atcggaatgg aaatacagga
     1921 atccaaatca gcggtagctc aaatgatcct ttcgaagcat ggcccgcgca taacttagtc
     1981 aagaactgta catcttttga aaatgccgat ccaggatttg aagatgccga tggatttgcg
     2041 gccaaactga caataggaga aggaaatgta tttgacggtt gtatcgcgta tcataatgca
     2101 gatgacggtt gggacctttt tgctaaaaat gaaacaggtt cgatcggaaa agtaatcatc
     2161 aaaaactcag ttgcttatcg aaacggctgg gtaccaggaa ttgagggaga aggtaacggt
     2221 aatggattca aaatgggcgg ttcttcttta acgggccctc atgaattgat caatagtttg
     2281 tctttcgaaa atcttgcgaa aggaatcgat tccaatagcg gaacagatat tatcgtcaat
     2341 agcagtacaa gttttaataa cggttcatat aatgtggctc tttatacctc ttcagcagga
     2401 aacacagact accatgcttc tggtatctta tctttccgaa cagagaatct tgagatgaga
     2461 gagcagatcc gcccattgaa tcaaaacgaa gatcaaatct accactcgtt gaactactat
     2521 tggaatgaac gagagaaaca atcagaaaat actcttggac ataccatatc aaaagattgg
     2581 atcgagtctc taaatacatg ttcgaaagaa ggtcagcagc ctgttacgcg ctatgaaaat
     2641 ggaagtatcc atgttcatgg actgatgcag attaaaccgg ggaatcgtca aacagaaaag
     2701 gaaagagtgg atggtcttcc attatctgta ggtgctattt ttgacggaga aacctcaccg
     2761 tcttcggaat atccagagta tgcaatcgta caagaaccaa atcagacaaa accaaatgaa
     2821 caaaataatc cgattattat acaagaagca aatggtgtgg cggagaatgc agaaggaaat
     2881 atcattgaac tgaagccttt tacagttttt gtgtcaactt tcacaccaaa tcaaggaacg
     2941 cctgtctttg aagatatgcg ttatttccca gaaaagatcc aaatcgttga aacacagtca
     3001 agtgctcaat tttctaatca taccgctatt ttttcagcac cttcaaaagg agaatatacg
     3061 ctagaagtca cttatcgttc ggaaatattt gatggggaaa aatggtctgc tcatacagaa
     3121 tctgttaccc agcaaaaagc gatcactgtg cgagaaactg ccgatgtgcc cccgggaaat
     3181 gacaaaccag aaagagaaaa tccaggaaat gaatcaccaa atggaaaacc aaatggaaaa
     3241 ccttctcatc cggataggac agcaaaagaa agtgaaaaaa atggcactca aggactccca
     3301 aaaacgggag aatttaataa cccgttgcta gcattagctg gaggaatttt gttaatcggt
     3361 gtagttgttt atgtcatgaa acagcgaaaa aaataagtgg aatttctcct tttatgatgt
     3421 aaaacaattg actgaggaaa gtaaaaatag aaggaaatga gaccaataga tgtctcactt
     3481 ccttctatct ttttatagat gaggttctcc ttgctgcttt tgattggctg ttcttattac
     3541 ttgatgcagg acagcttttt cacaatctct ccactaaacg gtgttcaatc agctgaccct
     3601 cggtattagc tcaacaaacg gcaaaatatg aggaactatt tttccgtttt tttctctaat
     3661 actcaggtca ggacgctgat ttcacaacct ctcgataaac ggtgttcaaa agccagtttc
     3721 tcggtagtaa gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttctta
     3781 ctacttgaaa ctaagcagct ttttcacaac ctctaataca tggtattcca gaagtaactt
     3841 cttcgaaaaa taagccgaaa tgtcacaaaa atttgagaag cgattttccc aaattccttc
     3901 ttatttcttg aagctgacca cttctggcac aacctcttac cagattcgca ctcttttttc
     3961 tggcgctaga tacatagcgt cttgttccgt gataccaaat gtttcataga attcgtcaag
     4021 gttttttggt tgcacgtttg ctcggagttt agcaggggca tggacatcga tttgcagtaa
     4081 aagttgttgg tattcttttt ttgcttttgt acgccaaatc gttgcccaat tgacaaagaa
     4141 ttcctcgata gaaagatctg attccgtttt tgctgcttcc aatgcacagc ttagaccgcc
     4201 tgcatcagca atattttccg aaacggttaa ttttccgttt acttttccgt cagcaaaagg
     4261 cagaccatcg aattcttcga tcattgcttg tgctttgttt tgaaattgtg taagatcttc
     4321 ttctgtccac cagttgttca agttgccata ttcgtcaaat aaagcgccgt tattatcaaa
     4381 tgcatgggaa atctcatgag cgataacagc tccgattcca ccataattag cagagcttga
     4441 ttgttctaga ctgtaaaatg gtgcttgaag gatcgctgcc ggaaagacga tcacattccg
     4501 aaatggatga taataagcat tgaccgtgtc agcactcatt tcccattctg aacgatccac
     4561 tggtttgttc catttgctga aacgatcttt taatgtgagt ttgctgaaaa caagagtatt
     4621 tgaaagcaaa gtaccaccct gatctgcagg tactgttttg aactggtcga ataatgctgg
     4681 gatcttatcg gggtagccta cttggatacc taagttattt aattttatga cggctttttc
     4741 ttttgttgca tccccaagcc agtcattttc ttctagacgt tttttataaa cggcgatcat
     4801 ttttttcacc atttgttcta cgtctttttt agcagcttcg ccaaaatatt tttttccgta
     4861 gtaatcgcca actacttgat cgaattgtcc agaggcaagg taatacgctg cttttttctg
     4921 aggcattgct tcgtctgttc cagacaatac gcgagaatag attccgctga cttggcggaa
     4981 ttcttctgat agataaccac taagagaacg tactgtttta atgatcatcc agcttttgaa
     5041 taacggaaaa tgagcttctg tcaggatgtc attcaaatga tcgaagtaca ctggatcagt
     5101 tacgatcact ttatctggag ttgtcccaat caaacccgtg acaagttttt tcaagtccag
     5161 tttatctgtg tatttaataa atgtatcaaa agattgcgga ttatacattt tgctgtaatc
     5221 tgcgttttct tctgcacttt ttacatgagg agcaatcaat ttatcaaatt gcactgcttg
     5281 ttccgtgatt gcttctgcct gacttttttc tttgccggca agctctaata actgaacagc
     5341 catatcaaag aaaacgccta ttagttgtgc accgttggga tggtctgatg cataatacgt
     5401 cttatctgga aggaataaag aaggcggata agcaaagaag gcgttcgttt gcgcattctt
     5461 catatctgca tctacatcca gattaaatgg caatggtaat gtatccaaca gccattctgg
     5521 aaattgctta ttcaattcgt cgaaagactg caatgattca actttttcga ttataggttg
     5581 aagcggttga gcatgcaact catttcttcg gtcataatcg tttgccagtt gataatatgc
     5641 aaggaaatgt ttcatttttt ctgaagacac tttttctgga tgggcaagca gatcgtctgt
     5701 ttctcccatc aaaagttggt cgatcccgtc aactaaatct tggaaaccgc ctgtagctgg
     5761 tttatctgca gggatttttg ctgttttttg ccattcttca ttgactgctt catagaaatc
     5821 ttgtttcaat cgttctttgt tcattcatcg atcgctcctt ttttgtttcg tgtgcccatt
     5881 atcttacaaa atgaaggaat taacaaatag aacataaaaa attgaaaatt cgaacaatca
     5941 aactgatttg tttttttcaa tccttcactt ataatgaaaa aaaggaggcg atcccaactg
     6001 aatcaagtag tggataaacc tttatatttc aaagtagata aaaaaattaa aaaattagcc
     6061 agcactcagc agctgcagtc aagaaaaagt aaattgcttt ttttagctct ggtttttgaa
     6121 gatcaaagtt atgtgattat cgatcaatca ggacatccgg tagaatattc tccagcaaaa
     6181 tatacttatc aagagggtct ttcttgtaaa aaatggaagc tgatcaatga agagccaatc
     6241 gagttatcta gatggatcaa cagaaaagaa gatattcccg ttttgatcga agaaaaaagg
     6301 tcaggaaagg aactcgcaaa ttgctgggtg ggattgccag aagaacgttt tttgaggtat
     6361 aagaaatggg ctaccccctc aggctatctt tgtggtactt acgcggcagc agttctcctt
     6421 gcctattatc aagactatcg taaggggtgg atgcttccaa atgagattcg caagaaaaat
     6481 acggctgatt cgttggtttt gacgaaggca ttgcgtagcc agatccaacc actaggactt
     6541 cctacgattc cttttcaggt tagtacaggg atttctagtt ttttaaagaa gaatggcaat
     6601 catgagcgtg cacgagcaac tcttctaggt tcatggcagc gagctacaaa acgtatacgt
     6661 gaaggcaaac ctgtgatgat tggtattttg aaagtacttg gcagtacata tggcaatcat
     6721 tgggtgacag cgtacgctta ttttgaaact gaaacaggag aacgttatta caaagtacac
     6781 gacaactggg gcgattatca taaagtcatt cctgccagtt ggagtaacgg aacagtttca
     6841 cttccttaag aatttgctga aattaacaga aaatcttgga aaaatatgtt aaactatcga
     6901 agaatagaga agttttgggg aggactacat gagtaataag tatcaggatg acacgttgaa
     6961 gatttttagt ttgaatggca accgtccatt agctgaaaaa atcgcgaaag tgttcggaac
     7021 agaattaggt aaaagcgtcg tgaagcaatt tagtgatggc gagatttcca tcaatatcga
     7081 agaaagtatt cgaggggatc atgtgtatat cgttcaatca acgaatgcac ctgtcaatga
     7141 ttactatatg gaactactga tcatgatcga tgcaatgaag cgagcaagtg cgaaaacgat
     7201 caatgtcgta ttgccttact atgggtatgc taggcaagac cgcacggcga aacctcatga
     7261 accgatcact gcgaaattga tcgctaactt gatcgaagaa gcaggggcaa ctcgagtact
     7321 gactttagat ctgcacacgg ttcaagtaca aggattcttt gatattcctg tagataattt
     7381 atttacgatg ccgttgtttg ctcattatta ccgtcaatta ggtctgacgg gagatgacat
     7441 cgtagttgtt tcgccaaaaa acagcggtgt tcagcgtgca cgtagtcttt cagaatattt
     7501 gaacagcaca ttagccatcg tagatcatgc agatgatgct tcaactgagg gtggttatgt
     7561 gatcggtgat gttcaaggaa agacatgtat catggtagat gatatcttga acacaggagc
     7621 aacgcttgca cgagcagcaa atgtattaaa agaaaacggc gcaaaagaag tatatgcctg
     7681 tgcgtcacat ggattattgt ccgatcatgc gaaaagtatt ttagacaatg caccaatcaa
     7741 agatatttgt atcactgatt ctgttttcac agaagaaaat cgtcatccag aaaacttaac
     7801 aatcgttaca tgttcagaat taatgggtga agcggtcaaa cgcatccatg aaaatacacc
     7861 gatgagtccg ttattccgtc tggaagaaaa agaattcgaa taaacaaaaa aaagagggaa
     7921 agaagttgtg acagaagtga gcagcttcaa ggagctgcga caagactttt gtcacagctc
     7981 cttttttcta tgcccatgaa acgtcctcta gacaaaaatg agctcttctt tggtttgttc
     8041 gctattttat gctagaatgt gcctagcaat aatttggaag ggatgaatga agtgaaaact
     8101 atttatctgg atcatgcggc aactaccccc atgcatccac aagtaattga agcgatgacg
     8161 gcaatcatgc aagacacgtt tggaaatcct tcaagcatac atggatttgg tcgccacgcg
     8221 catgaaaaat tggaaacagc aagacaaatc attgcagaca gtctgcaagc aaaacctcac
     8281 gagatcatct ttaacagtgg gggaacagaa ggggataata cagctatctt agaaacagcg
     8341 ttctcgcgtc aaaaagaagg cagacatcta atcactacag ctatcgaaca tcctgctgtt
     8401 cttaatacca tgaaatattt agagacaaag gggtttgacg ttacatatct gcctgtagat
     8461 gaaaaaggga atctgtcgat cgaagaggtc aaaaaagcgt tacgagaaga tacgatcttg
     8521 gtctctgtga tgtacgggaa caatgaaatc ggaaatttga tgccaatcaa agaaatcggt
     8581 gaacttttag cggatcatcc agcttatttc catacagatg cggtacaagc gtacggaaac
     8641 caaacgatcc atccagaaga attaggaatc gatttattaa gtatctctgc tcataaaatc
     8701 aatgggccaa aaggcgtcgg tttcttatat aaacgagacg gagtaaatat ccctgcattg
     8761 cttttaggcg gggagcagga agaaaagcgg cgtgcaggta cagaaaattt agcagggatc
     8821 tggggtatgg caaaagcagt tgaactgctg tcacctgaag aacgtgaagc acgtaacaac
     8881 aaatataaag gattccagca attgattctt gatacattag aagcaaacga aatcccttat
     8941 caagtaaatg gtgatttaac gaataaactc cctcatgtgt tgaatcttcg cttaattggc
     9001 gaagcaaatg atttgctact gatgaagctt gatcttaatg gggtcgctat ttctactggt
     9061 tcagcttgta cagcaggaaa tatcgagcct tcacacgtat tagaagcaat gtatggagaa
     9121 gatacaccga tactaaaaga atccatccgt atcagctttg gtttgggaaa taccgaagac
     9181 gacatccagt atttccttga tcaattagtt cgtgctgtca aaaaataact agtgaagtca
     9241 ttcttatatg aacagatgtc tgagagaacg ccttcatttt ttagggaact taactgaaaa
     9301 acctagtaaa aatcaggata aacttttata ataaagagaa actaatttta tcgaggtgaa
     9361 aatcatggca tttgaaacaa cagcaacggt actgggcgca ccaattagct atcgtttgca
     9421 tcctgatgca aaaagatata cattgcgcga taatggattt acagaaacaa acggaggcaa
     9481 ttatcaattg atccgaccat tagatgccac gccgcaaagc aaagaaggat tcaaattaaa
     9541 aatcactgta tcaaaagata ttcaaacatt gaaaatgtcg atcactacag caaatggatt
     9601 gaaagcagtc aatattttca aagatccaaa acagaaaatg agccaagata aattttattt
     9661 tctgatggat gggatgatta gccgtgggtt attcgaaaaa gtcgaatgat ctgtggatat
     9721 aaaaagacaa ctagttttga aacggcctat tgttagatga ctaactttgt aaggacgacg
     9781 caaattagtt gtctttttta tttttaaatt taacacatgt tgttattttt aatattttgc
     9841 tgatttattg tttctaagta aagaaattta aattgaaacg attgttattt agggaaaaag
     9901 gtattttttg aaaaaagaat cccgtataat gattgttgtg caaacgctat acaaaatgaa
     9961 aaagaaaaga gggatgttta tggaaaatga acagtcggtc gttttaacga attggaagag
    10021 aaattatttg ttctttttat cgggacagtt tttgtcagga atcactagta tggtcgtcca
    10081 atatgcgatc atctggtatt tgacaagaga aaccggttcg gcgacaattt tgagttttgc
    10141 tactttacta ggaatgattc ccatggtttt actaagccca tttgtaggac cgctagtgga
    10201 tcgctgggat aaaaaagctc ttttgattgt cacggatatt attgtagcga ttttcgcgct
    10261 tattttagca gtagtcggaa cgatttcaga atccttcccg atttggctag tttttgtgtc
    10321 tttgtttatg cgttcggtcg cacaaacatt ccaaatgcca acgatccagt cgattatgcc
    10381 gacaatcgtt ccatcttctc acataacaag gacgaatgga cagttaggaa tggtccaatc
    10441 agcgaatttc atcattgcgc cagctctagg tgcggctttg ttttcagtcg ttcctgtcaa
    10501 ttaccttatt ttattagatg tattgggggc agtttttggt gtcggcttat tgatttttgt
    10561 gaagatccca aaagtttctc ctgaaatatt ggaagttccg cttactattt ttaaagatgc
    10621 aaaatttggg ctgcagcaat tgatggataa taaagggtta tggtacataa cgatcaatgg
    10681 tgcatttgtg atgctgttgt tcatgccagc aatcagtctg tatccattga tgacgctaga
    10741 ttactttggt ggttccgttg gacaggcggg agcagtggaa gtagtctacg cagtcggaat
    10801 gctcttaggc ggtgcactga tcagttttat aggaacatgg aaagaccgaa tgaaacctat
    10861 tattatagcc tacatcatta tgggcctgac gatcggtgca agcggattgg ttccaaacga
    10921 tagtcaaggg ttcttgtact ttcttatctt gaatgcagga gcaggttgtg caacgccata
    10981 tttcaataca ctgctgatgg caatgatcca gcagagttat gaatcgaatg ttttaggtcg
    11041 tgtcttaggg aacttcaact ctttgatgaa cctggcaggt ccaattggat tattatttgc
    11101 aggaccatta gctgatcgtt taggggtaga aaaaatgttc ttgttttccg gaatcgggat
    11161 tttattatgc ggaatcgttt tatttttaac gtcagctgcc cgaaattatg ataaagaatt
    11221 acagaaaaaa ctggtaaaag aacaccatga gcaaaaagac gaatagtatg gaatggagtt
    11281 gtgacagaag tcttgttaca actccatttc tgatcctaat gttcctgtgg caataagcct
    11341 actcaaatga aaaatatgag taattatctt tatttgttta atacttgata tagggcagtt
    11401 ttttcacgac ctatcgtact tcctctttgc ttcatagatt tctttgattt gttgctcaga
    11461 taacggagtg ccaaatggta gtttttcctt taagagttct ctttccggtg cgtctacgcc
    11521 tacattgaca tcgttctcga taggtacgct gtaatgatgg atatgtccat gaagattgcg
    11581 gatagtgtcg ttaggcccga gcaacatcgg ataatgagtc aagtaatatt gttgatggtc
    11641 gaatttaacg atggcaccta catcataaaa ataaaatttg ggacttccgt cagtaagcct
    11701 aggatcattt ttttctaaat aacggaaaaa agcgcgactg tcatggttcc ctttgatcaa
    11761 gtggattgtt ccttgcagtt gagtgaccag ttccaaaatt tctgcatttg ctttttcata
    11821 ttgcggatgc atagcaaggt cgcctagatg ataaacatgg tcggtttctt ttattactgc
    11881 attccagcta gtgatcaact gttggttcat ttcatccaca tttgcaaaga gccgcggagc
    11941 aaagtcgttt tttcctaata agcgttcatg gaaaaagtgc atatcactga taaagtaatt
    12001 catgtatctt ttgcctccct tttttcttag agtaaacaaa atcacttatc tgagcaactc
    12061 ataacatcta aaaagaaaag gttgtaaatg agacgtgcag ctttaagcga taagaagcaa
    12121 ttttgaaaaa tagtttttca caatttataa aattattcct tattgccgaa aggctggccc
    12181 tttttatgag tatgccaatt ttttatatcg aatgctcaaa agatgagtga ttttttggtt
    12241 gactatcatg tttgtgtact gaataggtcc agtagaacag tatttttacg acttgataat
    12301 taagggatga aattttcaga aatatgacgt ataatgtaaa atactgaaaa gttgcgacct
    12361 tcaactcgta gatgtttcag aatctaattg aaatgatggt gatatcatga cagacaacag
    12421 caaaacacgc gttgtcgtag ggatgagcgg cggtgtcgac tcatctgtaa cagcactttt
    12481 gttaaaggaa caaggatacg acgtgatcgg aatattcatg aaaaattggg atgacacgga
    12541 cgaaaacggt gtatgcacgg ccacggaaga ttataaggat gtagcgaaag tagctgctca
    12601 aatcgggatt ccttattact ctgtgaattt tgaaaaagaa tattgggatc gtgtatttga
    12661 atatttccta gccgaatacc gcgcaggacg tacacctaac ccagacgtga tgtgcaacaa
    12721 agaaatcaaa ttcaaagcat tcttggatta tgcaatggat cttggcgccg attatgtagc
    12781 tactggacat tatgcgcaag tcactcgcga tgaaaacggc gtggtccaca tgcttcgtgg
    12841 agtagacaat aataaagatc aaacttactt ccttagccaa ttatcacaag aacagctttc
    12901 aaaaacgatg ttccctctag gcggcatgga aaaatcagaa gtacgggcaa ttgctgaacg
    12961 cgcaggcctt gcgactgcta agaaaaaaga ttcaacggga atctgcttta ttggtgaaaa
    13021 gaacttcaaa caatttttga gcaactatct gccagcaaaa aaaggaaata tggtcactct
    13081 tgatggtgaa gtcaaaggac aacatgctgg cttgatgtat tatacgattg gtcaacgtca
    13141 aggactaggg atcggtggcg gaggagattc gcaagaaccc tggtttgttg taggaaaaga
    13201 tctagcgaca aacacattat atgtggggca agggttccat catcctgcat tgtatgccac
    13261 aagcttggat gcaagtgaaa tccactttac gacgaatgaa ccaatgccaa aagaattcaa
    13321 gtgtacagcg aaattccgtt atcgtcagca agacgttcct gtgacagttc gcttattgga
    13381 tgataatcgt gcagaagtcg tattcgacga acctgtacgt gcaatcactc ctggacaagc
    13441 agttgtattt tatgatggaa tggaatgtct gggtggtggt ttgatcgacc acgcatatca
    13501 agaaacaaaa gttttacaat acgtttaagc gatattaaat gaatcaaaac agctatggct
    13561 aacaaaagaa acaccttttc ttttggatag catagctgtt tttttgcaat aaaattaatt
    13621 tttattcatt ttatacttga aatacttgtg taaaaagttc ataatatcca ataatatgaa
    13681 taaagttgct ttttgttaag caagagcagt tgcaaaatag aagggaatct tttgcaagtg
    13741 atttgttcaa ttttataagt gagggtgatg gaaatggaaa agaaatcgac gggtattagc
    13801 aaatggggac tggttgctct ggttgtcagt tcgtcaatcg gaagcggtgt gtttgggatc
    13861 acaagtgatc tggctagttc tgcagctcca gggccagcga tcctttcatg gataatcgtt
    13921 gggattggta ttttagcttt ggtattatcg ctgaatcatt taggagaaaa gcgtccagat
    13981 ctagatggtg gtattttcgg ttacgcggaa gcttcatttg gtaaattagg tggcttcatc
    14041 agtggttggg gttactggtt gtctgcgtgg ctgggaaatg ttgcttttgc aacgatgctg
    14101 atgagcgcca tgggagaatt tttcccgata ttcaaaggtg gacaaaatgt tccttctatt
    14161 ttatttgcaa gtatttttat ttggggtctg actttattag taaacaatgg agttgaagga
    14221 gcgagcgtca tcaatacaat cgtgacgatc tgtaaattag tgccattatt cctattttta
    14281 atatttgtat ttatcgcatt taaagtgaat atattcactg ctgatttttg gggtaacgta
    14341 tctgataatc tagtgaatgg aacagggcag caaggaacga tctggttgca gattaaaggt
    14401 tgtttgatgg ttatgatgtg ggtctttgta gggatcgaag gcgcaagtgt tttagccaac
    14461 cgcgcgaaga aacgttcaga agcccagcaa gcatcgatca ttggtctgct ttgtttattg
    14521 tttatctata tccttgcttc gatcttgcca tacggcgtct tatctcaaga agagttagca
    14581 actatttcac aacctgctat ggcaaatatt ttaaaaggaa tcgtgggaaa ttggggagca
    14641 gcgctgatca atatcggctt gattatttcg atcattggtt cttggttgtc ttggacgatg
    14701 ctcccagcag aaacaacgat gttgatggct cgagacggtc tgttgccgaa aggttgggga
    14761 aaaacgaata aaaaaaatgc cccaacttat tcgttagttg ttactgcaat cttgactaat
    14821 ctattcttat tgactttctt agttactgat tatgcctatc aatttgccta ttctttatgt
    14881 acagcggcaa ttttgatttg ttatctactt gtcgggatct atcaagtcca gttttcttta
    14941 aaagaaggaa tcaaaaaaca agtagtgatc ggtgggattg ctgtagtttt tgaattgatg
    15001 ggcatcattt tagcaggatt ttcttatgtc cttttatgta gtattgccta tcttcctggt
    15061 ttttgcttct actggatcgc ttgtcgtgaa accaaacatc aaattacggg aaaagaaaaa
    15121 gtaatgattg ggctgatttg tgtggccgca ctgctttcaa tcgggttgct tgctgtaggc
    15181 tggatcaaga tataaggggg aaagaaacat gaaaatcgca aatttcggtg tagaagaatg
    15241 gctaaataaa tgggaaaaga aagcggtata tgatattgca caaagctcga tcgaagcatt
    15301 gacattagac gaactcgttg gcttagatgg gactagcgta agtaaatttt tcgaaaaacg
    15361 caaaaacgaa ccattggact atggctggat cgaaggatca gatgttttca aacaagaagt
    15421 cgcttctctt tatcaaaccg tcgatccgga aaacatctta caaacgaatg gagcgactgg
    15481 tgctaatctt ttagctctat acgcgttagt ggaagcagga gaccatgttg tttcgatgct
    15541 tcctacttat cagcaattat atgacatccc caaatcttta ggtgccgctg ttgactttgt
    15601 tcatttgaag gaagaagaag attggcagtt tgatttagaa cagctagaag ccttagtcaa
    15661 acctgaaaca aaaatcatct gtttgaatag tgcaaacaat cctacaggca cattgctaga
    15721 tcggaaaact ttagaaaaaa tcgcagaaat tgctcgtaca gtagatgcat atgtattgat
    15781 agatgaagta tatgcgcctt taacggataa aggtgaattc ttgtcgatcg tagatgtcta
    15841 tgaaaaaggt atagcaacca attccctatc caaaacctac tccattccag gtattcgtat
    15901 tggctggaca gctactggac ctgagttggc agaagttttt cgaaagtatc gagactacac
    15961 gatgatttgc ggcggagtcc tgtcagatga tcttgctgta catgcattga agaataaaga
    16021 aaaaatattg gcgcgaaaca aaaagatcat tacagagaac cttgtgattt taaaacaatg
    16081 ggtagccaat gaaccgaaag ttgaacttgt cgctcccaat tacgtctcta cttctttcat
    16141 taaattagcc atcgaagaag atgaccagac attttgtatc aatttattag aagaaacagg
    16201 agtactatta gtaccaggaa gtgcctttga tttaccgaag catgcccgac taggttactg
    16261 ttgtaaaaaa gaaaccttgg aaaagggatt attcctctta tctgagttct tgaaaaaaca
    16321 ggcctaaaca aaaacactgc aaggacagtc tgtgacaact gttcaagcag tgtttttcat
    16381 ttacgcttat tgtctatgag aaggagagtg ccctgaagct atggagactc ctgaagcttt
    16441 ttcttctgca gtttcttgct gatggatctc ttgtgtattg tgtcgtacaa gcaagaagtt
    16501 gaccaaaaca agaagagaag tcgaaagaaa gacgccgcga taaccaaatg cactggaaac
    16561 actggagccg atcataggcc ctactacgtt accagttgct tggaaagatt ggttataact
    16621 gaagatccgt ccagcagctt gatgaggcga atattttgtg atcaatgctt ggacagcagg
    16681 gagcagacaa gcatcagaga taccaatcaa aaaacgtaag gcagctaatt gccaaacatt
    16741 cgtgacgaat gccattggga tataaaccag aatcgcaaaa cccaaaccaa tcgcaagtat
    16801 tctttcactc ccaatcttgt cacctaagcg accaaatctt ggagcggcta tcaaagtggc
    16861 aatcccaggt atcgaagcaa tgattccgct gatcaacgta acattcccat gtccatgcag
    16921 caattggcga atgtacagac tgatgattgg actaatggaa ttgttggatg cttggatgat
    16981 catcgttgtg acgaacatac cgatgacgac gtgtggatat ttcaagtctt tgaaaatctg
    17041 tttggcagaa accatgtttt cttttttcac tgggacaaat ttttcatgga cgaaaaacag
    17101 actcaacaag aagacgaaaa ataagatgat ccctgtaatg aaaaaggtag ggcgatagcc
    17161 aaacgctgaa gcagagatcc cgccaagaag cggtccaagt aatgttcctg taacagaacc
    17221 agttgctaag gttccaagaa cttgaccact tttttctttt ggcgtcccag ttgcgacaag
    17281 ggcagtggca ttactgatat aaccagaaaa aatcccttga agcagacgca atgcgactaa
    17341 ttgatacaca ctagtcacgc aacccatcaa agaaatgacg attgccattc ctaatgaagc
    17401 acgcaacagc atcaattttc ttcctttttg gtcggctaat tttccccacc aaggagagat
    17461 aagcgtagtc accaagaaag tagatgaaaa agtcaatccg ctccaaaagt tcaattgtga
    17521 cgtactataa ttacctaacg tatctatgta aagtgacata aaaggcatga ctaaactaaa
    17581 tccgatacca gccataaatg ttccaaacca taagacaaac aagttttgtt cccaagtttc
    17641 tcttttccta aataattttt cctttattct tgcagccaat tagactcctt ctttctgaaa
    17701 attttcatct acagctcttt attataaact acaatatgta gtttatcaat gaaagtctct
    17761 tttttctctt tctttttcgt ggtaaactga aagagtgaag gatttatttt atgagaggaa
    17821 acatggaaat gaaaaaaaga aatctgtctc aagagaaaat catcgactgc tttcgagaac
    17881 tagcagaaga gatggatgtt cagcaaataa cctttcagca tttagccaaa cgcttggata
    17941 tcaaggctcc ctcgctgtat aaccatttca aaaatattcg tgatgtcaaa acagcgctaa
    18001 cagcgaaact tctgcaggaa cttaatgacc agctgcgacg ggctcttgtg ggaaaaagtg
    18061 gtcaggaagc actgcagatc tatgcagaaa cttatcgatc ctttgctttt tcaaatcaag
    18121 cagtgtatga gttactgatc agtgtaccgc ataccaatga agctgtttta ttagatggaa
    18181 tccatgaaac gaatcagatc attttgcaga tatttgaggc ttttcaatta acgaaagaag
    18241 aaaaagttca tcgaagcagg gaacttcgta gtatgatcca cggttatctt tccttacgtt
    18301 ttttaggata ctttacaaaa gaaccaacaa tcgatccaga agacagctat ttttggatga
    18361 tcaataactt tatcgctact ttgccttaaa caatcagatt catttgtaac aattttaaga
    18421 tgttcattaa atgacccttt tataagagaa aattcttgca actttcttga aaagtgacta
    18481 agatgagtct agaaaagctg aaagaaggag cacgagtatg tatcaagtaa ttaccatgtt
    18541 cggggacaat gagccttggt ggttttttga agactgggaa gaagatattg aagaagaaga
    18601 aacattcgag tctttcgaag aagcacgaca agcgtacgaa aaacaatgga acgaaatcca
    18661 tcaaaattat gaatatatca atgcaaaaag taactttctc agcgcctttt ggaatgacgg
    18721 agatgagcgc tggtgtgaag agtgtgacga tgatttacag caatacaagg gacttgcgtt
    18781 gttgaaaaat catcagccca tcacagtaga aagcagaaag gaattttatg aaacaactaa
    18841 ttctagcgga aaaaccaagc gttgcgaaag acctaagcaa ggtgcttggt gctaatcaaa
    18901 agcacaaaaa ttattatgaa ggacccaagg tgatcgttac ttgggccctt ggtcatttat
    18961 taggattgaa aatgccggaa gatctgaata aggaatggca gagctggcaa atggacacgt
    19021 tgccaatgat ccctaaaaaa ctaggaatca aaccacttcc taaaacagga catcaattaa
    19081 aagcaatcaa gcagctggct cagcgaaaag atgtctcaga agcagtcatt gcgacagatg
    19141 ccggaagaga aggggaactt gttgctcgct ggatcttaga gtatgtgcat ttcaataaac
    19201 cagtgaagcg gttatggatc tcttctcaaa cagataaagc aatcaaaact ggattcaagc
    19261 aactaagacc tgcgaaagaa tatgatgcat tgtatgaatc tgctcttgcc cgggcaaaag
    19321 cggactggct ggtaggattg aatgtgacca gagccttaac tgtgaagtac gcggataatt
    19381 tatcagcagg ccgtgtgcag acaccgacac tagcattagt gagagaccaa gaaaagaaaa
    19441 tcgagcagtt ccgtccacaa acgtatttca cgatcacgtt gcaagctgga aaagaacaag
    19501 ccaaaatgct tcaaaaaaat caatttgctt taaaaacaca agaagaagca caaaaacttg
    19561 ttcaattgat cagtacaagc aaaggaactg tcacagaagt ccaagaaaaa ttaaaaacgg
    19621 aatctgcgcc attgccttat gatttaacag agatccaaag agaagccaat caacgctatg
    19681 gctattctgc aaagaaaaca ttaagtcttg tacaaagttt gtatgagacc cataagatcg
    19741 ttacttatcc gcgtacagac agcaaatatc tgacaaatga catgaaaagt acgatgaaag
    19801 aacgattgca agcagtcagc gactttgctc ctgaggtcaa gcagtatttg aaaaacggag
    19861 ccatcgtcca acagacaaaa gtattccaag acagtaaagt gaccgatcac catgcacttt
    19921 tgccaactga aaacagagca cgttatgaaa aattaagtaa tgaagagcga aaaatctatc
    19981 aaatgattgt ctctcgtttt ttaggactgt ttgctcaacc ccataaagtc agccaaacaa
    20041 aagttacggt tgaatttggt aaagaacgat tcatttttcg gcaaaatcgt gtcgtccaag
    20101 caggatggaa aggcgaaact gaaactgaga cggaaacagt caaatgggaa aaaggaatga
    20161 gaatcaatcc cgatttcgcc atcaaaaaag aactatctgc accgcctaaa ccattgtcag
    20221 aagcaagctt gcttggtcaa atggaaaaat acagtctggg aaccccagct acgcgtgcag
    20281 agattatcga gaaactaatc aaatctgaac tgatggaacg tacccctcaa ggactgcaag
    20341 tatctcctaa aggtaagcaa ttattagtat tggtaaatcc ctctttagta acaccagatt
    20401 tgacagaaag ctgggaaaaa gaccttgaag ctattgctgc aggaaagaaa caagcaggaa
    20461 ccttcttgaa tggaatcgaa aaagaaacaa aacgcttggt gaatgagatc aaatcaagca
    20521 aacaagaata tcaagatttc tcgattaccc aaaagaaatg cccagaatgt ggggccaatc
    20581 tgcgcgagaa aaacacacgt gacggcaaaa tctatgtctg caccaattca gattgcaact
    20641 accgcagaag aaaagatcca aaagtctcta atcatcgatg cccgcaatgt caccggaaaa
    20701 tggaaatcat cgaaggtaaa aacggtgctt atttccgttg taaatacgat ggaacgacag
    20761 aaaaattgct tgataaaaaa gagcagaaga agaaaatgac caaacatgaa gaacgacgct
    20821 tgctgaaaaa atatgcacaa gcagaagaac cagaagaaag cccattagca gcagcactaa
    20881 aagcagcaat gaagaagtaa taagatatga aagaggttgt gatagaagtg gtcagcttca
    20941 agaaataaga aggaatttac gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt
    21001 tccgaagaag ctacttctgg aacaccattt attcggaaaa aaggactgtg acaagacttt
    21061 tgtcacagtc ctttttcatt taaaaaaatg tttacttcat ttgacgtata ttattggtga
    21121 ggcttcaatt catttggctt cactttttct gtttctctta tgatataaac aggacgctgt
    21181 ttcgtttcta agaagatttt cccaatatat ttcccgatga ttccaagaca taacaattgg
    21241 attccaccaa tgaacaggaa gatcgagacc atggaaggcc atcctgatgt aggatcacca
    21301 aacaacaatg cccgcaaaat aatgaacaaa attgcaatac cagaaccgac acaagacaaa
    21361 gcgccgataa atgaagcaat cgttaacggt gcatccgaga agttcacgat accatcaatc
    21421 gagtagttga ataatttcca gaaggaccaa gatgtttcgc cagccacacg ttcgcggttt
    21481 tcataagaaa tatattctgt tttaaatccg acccagctaa ataatccttt tgaaaatcgg
    21541 ttgtattctg ttaattctag gacggcatcc accatttgac gagtcatcaa gcggaaatca
    21601 cgagcgccat cgaccatttc tgtatcgctg attttattga tcaactggta gaattttttc
    21661 gcaaagaaac tgcgtatcgc aggctcgcct tcacgagtcg aacgccgggt gccgacacaa
    21721 tcgatatcag aagtagtgat ccggttgatc atctcaggaa gcagttcggg cggatcttgc
    21781 agatcaacat ccatcacggt cactaaatct cctgtagaag cttgcaatcc agcgtacaga
    21841 gccgcttctt tcccaaaatt tctggaaaat gagacaaagc ggacataagg ataagctgag
    21901 gctagcttct tgattgctgc taacgtaccg tcttttgatc cgtcgtcaat aaacaaatat
    21961 tcgaactcat gggcaccttt gaatttttca acctcatcaa aaaaaagagg aacagttttt
    22021 tcttcattaa aacatgggac aacaattgat actttcatcg tttctatcct ttcaatcgta
    22081 tgtttatata tggtttacat tcaaaattta ataaacctta aataacacct ttaaaatttg
    22141 attttacgta aaagaacagg tattcttttt gtaaactaac gtatttagtt taccatgttt
    22201 cctcggttta tcaaactttt aagaacaatt tttattagtt ggcaagtagt tttggataga
    22261 gaaaaaggag gaagttccag tctattgact aggagcaaac aaaatgaaaa tacacacacc
    22321 tatcggcttt ttatggttag atgcagatga atccggattg actgctgtat cttttcagcc
    22381 aatttcagaa agcgaatcag cgaatcagcg aattttaacg cgcacacaag aacagtttga
    22441 agaatatttt gcgggaaaaa gaaaagaatt tgatgttcct ttatctataa agagaggtac
    22501 gccttttcag caaaatgtct ggaaagcttt gtcgaccatt ccttatggag aaatccgaac
    22561 gtataaagaa gtagccgttg ctgttgatag tccgaaagct gtgcgagcga ttgggcaggc
    22621 aaatcgaacc aatccattgc caatcgttat tccttgtcat cgagtgatcg ggcaaaatgg
    22681 aaaactgaca ggatatatgg gaaacgcaat agatggggtt gaaatcaaac gccaattgtt
    22741 gttgttagaa ggatatctgc aaaattaaaa acagttgtga ttcggacatt gttccatgcg
    22801 agaatcacaa ctgttttttc atgttcattc taaactttga tcagccaaat aaatagtaaa
    22861 gccatcactg tttggagaag cccgacagct aatccataag ataagaagcg tgtaccttct
    22921 ttcatgaatt ttgcaaagtc taaacgcaga ccaattgccg ctaaagcagt gatctcaaac
    22981 cagctgctga taaaatgtgc cgattggtca ataactacag gtaaagaaaa caagctgttg
    23041 accaaacaaa agatcacaaa ccctaataca taccaaggaa gcggtaattt tcgttttttc
    23101 tgcgtatcag ttggcgtttc aaacagagct ggttgtttct tgtgcatgaa tttttcaaac
    23161 agatagacaa caacaacgag catcataata cgtagaatct taaatagcat ggcgaattcg
    23221 acaacagaat tgtcaagtaa gcttgcccca gcgactactt gaccaacaga ttggagtgta
    23281 ccacccaaaa gtgcactttt ttcaatgaga tttccaccaa acaactgtaa tccaagaaaa
    23341 gggagagtca acatcatcac agtacccagt agattgacta gtgtgatgat ctgtcctttt
    23401 tcttcctctt tcgcatggat ggaaggggcg attgaagcaa tagcagaaga accacaaaca
    23461 gcgtttcctc cagccatcat caaagaagaa gtatcgctga attgcagttt tttaccaatc
    23521 aagtaagcac cgatgatagt gactgacatt tgcagtaaca caaaaacaat tccttttaca
    23581 ccaatttgag caatcgtttg agctgtgaca gttgcaccta gtaaaaaaac agagaattct
    23641 aatagtttgc tttcagcaaa ttttgtgccg attgctagaa caggctgttt aaaaaaagta
    23701 tttcctaata aaatacccaa taaaatagca atagtcgctc cacctaactg tggcagataa
    23761 atagcaagaa gcctgctgaa ggcagcgatg gcaaaagaaa gcagtaaacc gggtaagatt
    23821 tcgcggaccg ctgaatcctt tacagatttt attggatgtg acaatgattt catttgatcg
    23881 gttcctttcg tcaataaata ctaaaagcat actcgaacat agtagcacta aaccagtatt
    23941 ttgacaaaag aacaagaggt cgtgacaaaa gtcttgtacg acctcttgtt tcgaataaac
    24001 ggtgttccag aagtagcttc tacggaaata agccgaaatt tcacaaaaat ttgagaagca
    24061 attttcgtaa attccttctt atttcttgaa gctgaccact tcttttacga cctcttggta
    24121 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccagtacaaa aacatgaaca
    24181 gctgtttttg tttggccgtt cttattactt gatgcagaac agctttttca caacctattg
    24241 aatcgattat taagcgattt ttttagaaac aaattttcca tgtttgctat gttctttaat
    24301 taagaaacgt acgtaatgag caagaacgta gaagacaact acacttgcaa acagacattc
    24361 cgcaactttt aagaaatcca atacgctact atagatacct aaactgttga agtaatgaac
    24421 agtagtaaat atagctgttg cagagataac aagcgggaat gtaaaggctg catagcttgg
    24481 ataaaaacga agcttgaaca aacgtggaag catagacagc acaacgaaat acaatacttg
    24541 agataaagcg aacaatgcta cgacaagata aaaattaggt tcagcaaagt ttttaagata
    24601 acctgttagg caaagagaac caggtgctgc aacgatcgta atcagcggac gtgtcggatg
    24661 agccatattt cttacgaaaa agacacgttt gatgatgatc ggcaataaga acgcatagca
    24721 gatcaatgaa atccaaaaga aaatttttcc aataagtggg aagaagttcc cggcagttac
    24781 agtgatgacc ccaaatccaa cgtaaatgat aaaccagctt ggataaattg cttcaattga
    24841 aagatcagct ttgataacat ggtgataatt gaaataaaag acaaggatca cttggaagat
    24901 aacagcgcac agccaaatga atttgacaat tggtgcaata ctttcatatg aaacaaaata
    24961 agtacaaata accatgagtg acatcgtaaa agttggtgaa acggaggcga tgattggatc
    25021 atgcaacgtt tgtttcgtgt gttcgaataa gataaacaat ttaacaaaaa tgaatatcat
    25081 aagaatcata gagataccgc cagttagatt gcctaaagca acatggtgat agtctttaaa
    25141 cagatttcca agggaagcta acccaaggat caacccgcag atcgggatcg gaataatttt
    25201 caaaaagttc ttcatatata atattctcct ttttatttaa tagattttag taccaagatg
    25261 aacgaataca ttgtaaaatg aataggaatg tatgattacg agcaagcaga cagctgattg
    25321 tcttcatcta agttactagt tggatcagca actgttttgt tgtacatttc tattatagat
    25381 ggttttttgt atatttaaat caaatagttt ttatagtaat tatcataaaa agttatggaa
    25441 gtggtgccat gtttaagtta ttaacgactt ttcgagcagt atatgaaaca agaaattttt
    25501 ccagagcagc agaagtactg tttttatctc aaccagcagt ctcgaatcaa atcaaacagt
    25561 tggagcggga attgaatatc gatctttttc aacgtaacgg aagaaaagag atgcttccaa
    25621 ctaagcaagc cgacttatta tatgaacgga cactggtttt gttagaagaa tgggaagaaa
    25681 ctcagcaaaa attgctcaat gaacaaaatg aagaagaagt atgtcggatc gttgcttccc
    25741 acacctttgc tgtctatctt ttgcctcagt tgatgaaaca tctgatcaaa aggtttccta
    25801 aagtgcattt tgagatcgaa atcacgaatt ctcatgaatc attagaacaa gtagcgaaac
    25861 atgaatccga ttttggtttt atcgaaaagc cattagagac ttccggcgta caaagatact
    25921 ctttgatgga agaccagcta gtcatcgctg gggacccaaa aagcaaattg tggcttgtaa
    25981 gagaagcaaa ctctggtgtt tttcattaca cgcagcgtta ttttgaagag atgaatatcc
    26041 aagataagaa gatgatcgtg aaaagtaacg atgtaattgt tgcgttgctg aaggaaggaa
    26101 tcggtcgctc gattttgtct aagcgctctg ttccagaaaa tgtgccaatg actccgttga
    26161 atttccatcg atatttttat ttcatccaac gaaccaatct ggtttctgat cagctgctag
    26221 agattgccga agagatcctt cgatacaatg aaagcacaga aacttctcac agcgaggaga
    26281 atctcagctg gtagataatt gcatgtatca aaaaaagttc tatgatacac taaagataag
    26341 cagtaggctt ttgtactgca tttgctcaag caactgcaca aacggaaagt taatttttcc
    26401 caaatgtcta tggaaagact ttccattatc taactggacc gctataataa tcagcagtat
    26461 ggtttttaag aaaagggatg aaacatggaa aaaaaattaa aagcttcgaa cgataatcgt
    26521 attgattatg gcgtgatttt accagtcttt ctgttatgtt tgattggtct gctctcatta
    26581 tatgtagcac ttagccatga tccgaacaat ccagcagtct tgaaaggcgt agggatgcaa
    26641 gttgtctggt atttagttgg agcggcagca atcgttgtca tcatgcattt gaattccaaa
    26701 tggatctgga aactgactcc ttacctttat ggtttggggt tagtggtaat gggactcttg
    26761 ctcaaattct atgatcagaa tttagctgct tcaacagggt ctaaaaactg gtttcgattc
    26821 ggtgcgttta ctttccagcc agcagagttg atgaagattg cgtacatctt gatgatggca
    26881 ctgatcgtaa ctaaacataa tacgcaagta aaagaacgga cgatgaaatc ggacttttgg
    26941 ctgattggga agctcttgat cgtgacaata ccggttttag cattgatcat ggcccaagat
    27001 gactttggaa cgatgcttgt ctttcttgcg atctttggcg ggattttcct gatgtctgga
    27061 atctcttggc gcatcatcgc acctgtagta atcttggcag tagtcgttgg agcaggaacg
    27121 atcttcttgg ttacgactga aggcggaaga gacttgcttt ataaagttgg attcaaatct
    27181 tatcagtttg cacgtattga ttcatggctg gaccctttcc atgatacgtc aggaatgagt
    27241 tatcagccgg cccaaggact tttggcaatt ggtaccggtg gcttgtttgg taaaggattc
    27301 aacgtaagca atatctatgt gcctgttcgt gagtccgata tgatttttac cgttatcggt
    27361 gaaaacttcg gctttatcgg tggtgcattc gtcattttct tgtattttat tttaatctat
    27421 cgtatgattc gggtgtgttt cgatacgaat aatgaatttt acgcctatat cgcaagcggt
    27481 ttgatcatga tgctgctgtt ccatgtcttt gagaatattg gagcaaatat cggattactt
    27541 cctttaacag ggatcccatt gcctttcatt agtcagggag gatcctcgat tttagggaac
    27601 atgatcggta tcgggttgat attatcgatg agataccaaa atgaagcacc tgcaagatct
    27661 tcaaggaggt aagaaaattg tacacctttt actggtaccc gaaatgttca acatgcaaaa
    27721 aagccaaagc gtggttagac cagcatggcg ttagttatca aaccgttgat atgatcaaag
    27781 aagtaccgaa ggcctcaacg attgagactt ggctagaaac gagtcaagta ccaatccgtc
    27841 gcctgttcaa cacaagcggg atgaaatatc gggaacttgg actgaaagat caagtaggcg
    27901 agtttacggc ttcaaaagcg agccaagtct tatcgacaga tggtatgctg atcaagcgac
    27961 cactgatctt acgagaggac cagcttgttg caattggatt caatgaaaaa atttatgaag
    28021 gagaactgat ataaaatggc aggcaagcat ttgaagaaaa aggacaacct ttggatctta
    28081 tttaatggaa aagaatactg tgttggtttg acaaatgaag cgcaagaaga acttggagac
    28141 atcacgtttg ccaatctgcc aaaagcagga caatcgctaa aaaaagatga acctctgatc
    28201 gaagttgaag cggaaaaagc ggtcaatgaa tttgccagcc cgctaacggg tacagtttct
    28261 tctgttaatg ataaaatcgg tgaagatatc aatatcttga atgattcaga tgaaatgaat
    28321 gcgtggatct taagtttcaa agatgttgat ccaaaagaat ttgacgaatt ataaaatcag
    28381 aaaatcactt gaaaaaagtt gacactgatt cttccagttg ctataatcat aagcaatcct
    28441 atatgaaacg agaaagcttt gaaaagaaga gtacatgtca cggacgtttg aagagagccc
    28501 tgtttgctga aaatgggtaa cgaaaatgag atggaagatg gtcttagagc agagtaagcg
    28561 agcgtaagca aacttactac gggagtgccc gttacagcgc gacagtatac ggaaagaagc
    28621 taagtccaag tactggaaga ggctgttatt gcgaagtaac ggtaaatcaa ggtggtaaca
    28681 cggaagttca agctttcgtc cttttgctta aggcaagagg gcgaaggctt ttttgtttga
    28741 aaaattataa gagaaagatg tgaatggcat gtcattaatc caactaacga atgtcaaaaa
    28801 gacattcaat gggaaaaacg gaaacatcca agccgtcaat gacgtttcac taaacgttga
    28861 aaaaggggat atttatggaa tcgtcggcta ttctggcgca ggaaaaagta cattagtccg
    28921 tctcctgaat ggacttgagc ttcccacgag cggcgaagtg atcgtaaacg atcaagatat
    28981 tacaaaatta aaaaacaaag aattacgtac attccgaaag aaaatcggta tgatcttcca
    29041 gcatttcaac ctgttatggt caagaactgt acttgaaaat atccagctgc cgctggaatt
    29101 agctggcgta ccaaagagca aacgtaaaga acgggcagaa gaactgttgc gtttagtcgg
    29161 gttagaagga cgcggacaag catatccttc acaactttcc ggcggacaga aacaacgcgt
    29221 cggtatcgca cgagcattgg caaatgatcc tgagatcctg ctttgtgatg aggctactag
    29281 cgcattggac ccgcaaacaa cagaagaagt attagactta ttgttagcga tcaataagaa
    29341 attgaatttg accatcgtct tgatcacaca tgaaatgaat gtgatccgca aaatctgcaa
    29401 caaagtcgct gtgatggagt tggggaaagt cgtagaagaa ggagacgtat tgactgtctt
    29461 cagacaaccg aaaaaagaag taaccaaacg ctttgtacaa caagacatcg aaccagaagc
    29521 tgattcgaca gaactattag ctgaactcat caaagaaaat ccaagcggcc gtttggcaac
    29581 tttgaccttt agcgaaagaa atgccaacga gccagtcatt tcccaagcga ttcgacgcta
    29641 taacatcgat gtcaacgttg tgtatggaaa gatcaaacaa gtgaaagaag gttcctttgg
    29701 ttcattgact gtcttgatga ctggtgaaac caaagaatta gatcaagcag aagtgttcat
    29761 caaagaacaa ggagtaggaa ttgaggtgat ccatcgtgga taaaagcttt tcagaaacat
    29821 atctagattt taaacaaatc aacccggaaa cgatgaatca ggctatcctt gatacactat
    29881 atatgacatt aatctcaatg gtcttagtga cgatcatcgg attagttttg ggattgatcc
    29941 tttactctat tggacgcaaa aacaaaccgt atgctcgaat catttatgga atcgtatcga
    30001 tcatcagtaa tattttccgg tcaacgccat ttatgatcct gatggttttg atcattccat
    30061 ttactaaagc aatcgtaggc actatgctag gtgcgcaagc agccattcca gctcttgttt
    30121 tatccgcagc accattttat gcccgtctag tagagattgc ttttcgtgaa gtcagccctg
    30181 gtgtactgga agcagcagat gcaatgggag caagttactg ggaaatcatc tggaaggtct
    30241 tgatacctga aagtattccc gcattgattt ctggattaac agttacaacg atctcgatga
    30301 tcggatttac tgcgatggcc ggcgcgatcg gcgcaggtgg acttggaggt cttgcttggc
    30361 aggaaggcta ccagcgtggt aatctaacag tgacattcgt tgcgacttta attattttac
    30421 taatcgtatt tatcgttcaa ggaatcggtg atttcttaac taagaaaaca gacaaaagat
    30481 aataggatat agggggatgt aggatatgaa aaagaaaatt tttggatttg cagcagcgct
    30541 tttgctgact gtaggattag cagcatgcgg taacaacagc gacagcaaag attcagcaaa
    30601 taaagcagac gacacgacat tgaaagtcgg cgcttcaccg acaccgcacg cagagatttt
    30661 ggaacatgtg aaaccattat tgaaagatga aggaatcgat cttgaaatcg tcaaattcga
    30721 tgactatgta ttaccgaacc aatctttgga agaaggcgac attgatgcca attacttcca
    30781 gcatattcct tatttgaaca aagaaatcaa agaaaaagga tatgactttg tcaatgccgg
    30841 tgctgtccac atcgaaccaa tgggcttgta ctctaaaaaa gtcaaagatg tttccgaatt
    30901 aaaagatggt gcaacaatca ttacttcaaa ttctgaatca gactggggac gcatcatcac
    30961 aattctacaa gatgctggct tagtcaaagt caaagatgga gtagatctgg aaacagcaac
    31021 attcgatgat attgaagaaa accctaagaa cttgaagttc aaccatacga tcgacccatc
    31081 acttttagct tctacgtacc aaaatgatga aggtgaccta gttgcaatca acgcaaactt
    31141 cgcttacggc gcagggttga atccattaga agatgccgta ttgcttgaaa aggacaactc
    31201 tccttacgtg aatattattg cgactcgtaa aggtgatgaa gacagtgaca aaatcaaaaa
    31261 actgattgaa gtgcttcacc gcaaagaagt ccaagaatgg atcacagaca aatggggcgg
    31321 ctctgttaaa ccagtaaagg ctgatgcaaa ataaggtatg gaacaaaaaa gatgagaaat
    31381 aagtctgtaa tggcttattt ctcatctttt ttatacctat tctaatttag aaatcaaaat
    31441 tgtttaataa taaataaaag atatttgatt ttagtaagga aggtcattgc gcttcattct
    31501 caattagtct aaaatgagtg agaacgaaag agaatttggg ggtataatgc atgtctgtct
    31561 tagaaataaa aaatctacat gtgtcgatcg aggaaaaaga aatccttaaa ggcgtaaacc
    31621 taacaatgaa aaccggagaa atccatgcca tcatgggacc taacggcaca ggaaaatcta
    31681 ccttatctgc cgctatcatg ggcaatccaa actatgaagt aacagaaggc gaaattttgt
    31741 ttgacgggaa aaatgtttta gacttggaag tagatgaacg tgcgcgactt ggcttatttt
    31801 tagctatgca atatccaagt gagataccag gtatcacgaa tgcggaattc atgcgtgcag
    31861 cgatcaatgc gaaacgtgat gaaaacgaca aaatttctgt tatggcattt ttgaaaaagt
    31921 tagatgaaaa aatggcttta ctaaatatgc cagaagaaat ggctgaacgt tatctgaacg
    31981 aaggtttttc tggcggagaa aagaaacgga atgagatcct gcaattgttg atgttagaac
    32041 ctacatttgc aattttagac gaaatcgatt caggtctgga tatcgatgct ttgaaagtcg
    32101 ttgctaaagg agtaaatgaa atgcgcggag acaatttcgg tgcattgatc atcacccact
    32161 atcaacgatt attgaactat atcacacctg atgtcgttca catcatgatg gaaggccgag
    32221 tagtcatgac agggaatgct gacctagcta aacgtctgga agcagaaggc tacgctggta
    32281 tcagtaaaga attaggaatc gactataaag aagaagaagc gtaaggggga gcaagaatga
    32341 aagaaaaaaa ctggctcgat tatcttgacg cggtaaatga tttttcttta agtaaaggcg
    32401 aacctgattg gatgagaacc ttccgtcaag acgcactagc gaaagcagat gaactgccat
    32461 tgccgcatat cgatcgggtg aaattccatc gctggtcgtt atttgatgtg aaagaaacac
    32521 aaaccatcag tgaaacaggc acgattccgg catttgatgc aatgaaagac aatcctgtgc
    32581 ttgtccaaca aggttcatgg acgatttttg aacaactgcc tgtggaatta gcagaaaaag
    32641 gagtcatttt taccgacttg ttcactgcaa tgatcgaata tccagaacta gtccaagaat
    32701 attatatgaa aaaagcagta aacatgaacg aagaccaatt gactgcactc catgtagctt
    32761 ttatgaacag cggtattttc ctgtatgtgc ctaaaaatgt cgtgatcgat gaaccactcg
    32821 agtccttatt cattcaagat ggcgcatccg atgagcactt cttcaagcat gtgttgatcg
    32881 tggcagatga acatagtgaa ttttcttatc tggaacgttt ccagacaacc aaagaacaag
    32941 tagcaaaatc atcaggtaat atcatcgtgg aagtcatcgc taaagctggt tcaaaaatca
    33001 aatattcagc cgtagatcaa ttaggagaaa atattacttc ttatatgaat cgccgtgggc
    33061 acattttacg agatgcttct gtcgactggg caattggtgt catgaatgac ggtcatgtga
    33121 tcgctgactt tgactcagat ttagcaggag aaggtgcaca cgcagaagtc aaaatcgtgg
    33181 caatcagctc gggcagacaa atccaaggaa tcgatacacg agtaaccaat aaagcccctc
    33241 atacgatcgg gcatattttg caacacggag tgatccgaga aaaaggaaca ttgaccttta
    33301 atggtatcgg ccacatcttg aaaggggcaa aaggtgcaga tgcccaacaa gaaagtcgtg
    33361 tcttgatgct ttctgataaa gcacgtggtg atgcgaatcc tattcttttg atcgatgaaa
    33421 acgaagtaac tgctggacat gctgccagtg taggacgagt tgatccagaa gaaatgtact
    33481 atctgatgag tagaggattg cataaagaag aagcagagcg cttagtgatt cgaggattcc
    33541 tcggttctgt cttaacagca atccctgtag aacaagttcg gaaagaatta gtcgaagtaa
    33601 tcgaagggaa gttgaatgga tgatcgatgc taaaaagcta cgcaatgatt ttcctatctt
    33661 atttcaagac gtcaatgatg aacctttagt ttatctggat aatgcagcga cgacccaaaa
    33721 gccaaaacaa gtactccagg cattagcgta ctattacgaa cacgataacg ccaatgttca
    33781 tcgcggtgtc catacgttag cagaacgcgc gacccatgat tatgaacaag caagagaaaa
    33841 agtacgggcg ttcatccatg caaaagaaac agcagaagtg ctgtttactc gtggtacgac
    33901 gacaagcttg aattgggtag ccagaacctt cggcgaccgc tatatcaaag caggagacga
    33961 gatagtgctc tcttacatgg aacatcattc taatgttatc ccgtggcagc agttggcaaa
    34021 acgaacaggt gctgtcttga aatatatcga actaacagaa gaaggcttcc tggatatgga
    34081 ggatgcagcg aaaaagatta cggacaaaac caaaatcgtc tccattgctc atgtatccaa
    34141 tgtcttaggt gtcttcaatc ctgttcaaga attagctgaa cttgtccatg aaaacggcgg
    34201 ggtcatcgta gtagatggtg ctcaatctgc cccgcacatg gccattgatg tccaagcact
    34261 agactgtgat ttctttgctt tcagcggaca caaaatgtgc ggaccaacag gtattggtgt
    34321 tttatatggt aaacgaaaat ggttagaaga aatggaacct gttgaatttg gtggcgaaat
    34381 gatcgatttt gtcgaattat acgacagtac atggaaagaa ctaccttgga aattcgaagc
    34441 agggacacca aatatcgccg gcgctatcgc attaggccat gcaatcgatt atttgcaaaa
    34501 actcggtatg gacaatatcc atcgatatga agaagaacta gccgcttatg tgttgccaaa
    34561 attacaagca attgatggat tgactgttta tggtccacaa gacccgaatt ttcggacagg
    34621 agtcgttgca ttcaatttgg atgggttaca tccccatgat gtcgcgacag ctttagacat
    34681 ggaaggggta gccgtcagag caggtcatca ttgtgcgcaa ccgctattga agtaccttga
    34741 agtatcagcg actgctcgcg cgagctttta tttctacaac acaacgcaag acgccgaccg
    34801 tttagttgaa gcaatcatgg caacaaagga gtttttccaa catgggacta tctaaattag
    34861 acaatttata ccgtcaagtg atcttggatc attcttccca tccgcatcat cgtggaagct
    34921 tgactgactc gacaagcaag atcgaattga acaaccccac atgtggagat gtgatcgaat
    34981 tgcaagtcga tgtaaaagac ggaatcatca aaaatatcgc ctttgacggc agtggctgct
    35041 ccatcagtac ggcaagtgcc tcgatgatga cagacgccgt catcggaaaa tcaatagaag
    35101 aagcagaaca tttaactgta gactttctac agcttgtcca aggaaaagaa gtcaaagatc
    35161 ccgaccaatt aggcgatgct gctatgcttg gtggtgtagc taaatttccc gctcggatca
    35221 aatgtgcgac acttgcctgg aaagcactag gaaaagcaat ggaaaacgat ggtaccgcag
    35281 ctttccatca ttaccacaag gaagaggagt gagaacatgg gcgtaccaga attagaagaa
    35341 tataaattcg ggtttcacga tgatgtcgaa ccggttttca gtacaggaga aggcttgaca
    35401 gaagaagtga tccgtgagat gtcaagaatc aaaggcgaac cagaatggat gcttgaattc
    35461 cgtctgaaat ctctagaaac atttaacaaa atgccaatgc aaacatgggg ccccgacctt
    35521 tcagacatcg atttcgatgc catcaaatat taccaaaaac caagtgacaa acccgctcgt
    35581 gactgggaag acgtaccaga aaaaatcaaa gatacatttg aaaagattgg tataccagaa
    35641 gctgaacgag cttatctagc cggtgcttcc gcacaatatg aatcagaagt cgtgtaccat
    35701 aatatgaaag atgaattcga aaagttagga atcgtcttta ctgacactga ctctgctttg
    35761 aaagaatacc ctgaattatt caaagagtac ttttcaaaac ttgtaccgcc aaccgacaac
    35821 aaattagcag cgctgaattc agccgtatgg tcaggtggga cctttattta cgtgccaaaa
    35881 ggcgtcaaag tcgatgttcc attacaaacg tacttccgga tcaatgcgga aaacacgggg
    35941 caattcgaac gtacgctgat cattgttgac gaaggagcaa gcatccacta tgtcgaaggt
    36001 tgtacggcac ctacttattc aagtaacagc ctgcatgccg ccatcgtaga aatctttaca
    36061 agaaaagatg cgtattgccg ttacacaacg atccaaaact ggtcagacaa tgtgtacaac
    36121 ttagtaacca aacgagcaaa agcatacgaa ggcgcaaccg ttgaatggat cgacggaaac
    36181 ctaggtgcaa aaacaacgat gaaataccca agtgtttact tagatggaaa aggtgcacga
    36241 ggcaccatgc tctccatcgc ctttgcagga gcaaaccaaa tccaagatac gggtgcaaaa
    36301 atgatccaca atgcaccaaa tacatctagc tctatcgtct ctaaatcaat ttcaaaagac
    36361 ggcggagaag taaactatcg aggacaagta acctttgcca aaaacagtgc gggttcgatc
    36421 tctcatatcg aatgtgacac gatcatcatg gacgaatggt cgaaatccga tacgatccca
    36481 ttcaatgaga tccacaacag ccaagtctct ttagaacacg aagccaaagt ctcaaaaatc
    36541 tcagaagaac aactctacta tctgatgagc cgaggcctga ccgaatctga agcaactgaa
    36601 atgatcgtca tgggctttgt tgaaccattc accaaagaac ttccaatgga atatgcagtc
    36661 gaactcaatc gattgatcag ttatgagatg gaaggcagtg ttgggtaaac ctgttatagg
    36721 agctagaacc cttgatatat aagggttcta gcttttttgt tttgcctgta tactagaaat
    36781 gaccagattt tgaccagctc ttttactgat gagccatcat atcagcaaag tcttgacctg
    36841 ttttcttagg tgcattattt gtaatatgtg catagatgtc taatgtcact ttactatttt
    36901 catgccctaa tcgttgttga actaccttga cagattctcc tgcttctaat aataaactag
    36961 catgtgtatg tctaaaccca tgaggtgtaa tacgaggcaa gttatatttt ttcaaaatat
    37021 atcttaacca atcattaaca gtgtttggaa tgtatagagt gtttttaact gttgtaaaaa
    37081 catgttgctc tttagagcta gtattgtatc caagttttaa atactcttct ttttgctgta
    37141 atttccagtt gttgaggata gaaagagtct cagtatctaa agagataact cgtctagaag
    37201 ctcttgtttt cggagattga attataactt taccaaattc atctacagca agagttttat
    37261 tgacatgtag ttctttatta aacgtgtcta tgtctttcca ctgtaaagcg agtacttcac
    37321 tctttctcat acctgtgtat gccaataatc tgaagaaggc gtattgtttc atgttaccaa
    37381 aatctttaaa agcatccagt aaattattaa gctcctcttt tgagtaaaat ttacgatttt
    37441 tctcatactc tttttttcgt ggaagaagtg ttttttccat aggattattg tcaatatatt
    37501 gcattgcaac cccgtagcgt aaaatttgtg cagtctgttt tctcatataa tcgtaacttt
    37561 catatttact gtgccattca tctaccactt tttgacaata tgggacagag atattcgata
    37621 gtttaagctt accaaatgct ggaagtattt gattatttgc gtatcttact gagattgcga
    37681 ttgttgaagc tttaacactg gtacgctgat gagttatcca ttcattatac aggtcttcaa
    37741 atgtcatatc aggtgctttc tttttaacac cgtataatat ttgatgtctg tactcgtcat
    37801 aatctttttg agcttcttgt ttgctactaa aacctcttct cgtagtattt attttttcac
    37861 cagttcgtgg atcggtggca atgtgtcctt tatacatcca cgccacttga ccgtttttct
    37921 ttttgtactt tgatattttt gccatgaaat catctcctct tagtttacaa ggagtactta
    37981 ccactagatg gtatcacctc ccttcatttt tttcttagtg tattttttcc caattgatga
    38041 aatcgtttgt tctagcttgt ttatattatc agtaatttct ctcacagctt cttcgggata
    38101 agacatgtct ttcaattgaa taggtagcca ttcatctacc attctttcta gtagttcttc
    38161 tatactgcta gtgaggaaaa gaatgttatt gtcaaattct tctacaatag cattgatgaa
    38221 taattcttct atttttaaga tattgaaaag actaattttt tcatttttaa ctttattaca
    38281 agtcttgtta gcaataaatt cttgcatctc tggttctaat ttggaatatc tatccagcca
    38341 tgtatctaaa ttagagtgta ctggtgggaa ctgttgacga agatgctgga acaggttttt
    38401 atctggaaat gttataccat ctagtacaat gctatctcta taaagaataa ttcccaaaat
    38461 atattcttct acattgccac aaactaactc ctctatactc atatttccca aatcagcaat
    38521 tcgggataat cgctctggta ttggtaaata ggttcccttc tcccattcgt agatagtttt
    38581 tttgtcaatt aattgaccgt tgttaatcat aagaccaaat gctggttgtg ttaatttttt
    38641 actctttctt atagctttta tttttgttcc gagtatcttt tttcgttcta aataatttat
    38701 tggtggacgt tttatcatat tgaccacacc tcctaaaatt agtatacctc gaaaaagggg
    38761 tgttgtaaat aaattttaca aagatattga cacctctttt ttatcgaggt attatgtgta
    38821 tgtcgatgaa ctggtaatca ttttttgttt gattaatcgg ttaataacaa aatactttta
    38881 ttttgagcaa aaaatacgaa gataattctt agaaaatttg ttcaagcaat cacgaaagga
    38941 gggggaaata tgtctctaca aatcattgat agcattgttt ctatacaagt tgaacaacaa
    39001 gtgaaagaga taattacagt gctaaagcct atgctagaag acattatttc tggagtcact
    39061 ctagagtata tggactatca gcaggcaagt gaatatctag gtgtatcagt aggaacgatt
    39121 agaaaatatg tttctcaata cgggttacct gttataaaaa ttgatacggt cattagattt
    39181 aaaaaatctg atattgatag tttcatggaa cggtataaag gttgagtaaa caaataagac
    39241 taaaacatat agtaataaag agtttgtttt tattatctgt atgttttagt ctttaacaga
    39301 attttataag gaggaaacac aaatggaaac atcaatgatt aatattccaa tttatgtaga
    39361 taaagaaaaa ttattagaga agccagttac aaaaaaagaa aaaactgatg taagagatag
    39421 gtgtttagat aactatagag aagttacaag agatgagttt gtagagatac taaattcatc
    39481 acaatcattt attccaagca agacaaagaa taaaggcaat gacaaggaga gttttgtgga
    39541 aaccagagta attattctgg atgtcgataa cactgttaaa gatgagaagg ggaaggtagt
    39601 agatttgagt aaagatgata gtagatactt aagcatagaa aaggttttat ctatcaattt
    39661 agttcataat tctgcgtttg cgatacaaaa aagtattcgg tactctgaaa atctagaaaa
    39721 atacaagatt gtctttctat taaaagaagc aattactgac tatggtaaaa tggttgctgt
    39781 ttacgaatac ttgaaaaaga acatccccgg gtgtgatgac aatgtaaatg cttcgaatag
    39841 aatgtttttt ggaggatata agtcagatgc agtgataata attaatgaag acaacatgtt
    39901 agatattact gagcttccga ttgattttac catgacatgt tctaaaaatt gtcaaagtga
    39961 ttcagggaga gagtatgaac tagtcaatga aaccgatttt gttaagttga taaaaaatga
    40021 cgacaaagaa gaaatgaaac agtggtttaa agactgcctg tttgattcct ccgacatgtc
    40081 ttttaatgag atttatgaaa gattgttaac cattgatatg aataaattac ttaagtcaag
    40141 taaaaactta agatgtctgt ttcacaacga tcataatccc tctgctagta tttttaccag
    40201 taaaactggg cattccattt tttattgtca ttccagtaat tgtgatatca gtgaaaattt
    40261 tattggagtt gtgatgctaa ttttaaagaa agacagtcgg gtagaaacat ttaattggct
    40321 attagagaac ctagacttgt atcctacaca ttttcgaaaa ttgaaagagg aatcaaaagt
    40381 actttttgac actcttgaat caatgaaaga caaacttttc aggacaataa ggaattatct
    40441 agaggaaatg cgacgtgtct atgatgtctt gctttctgat gttaattttt ttgatgatag
    40501 taaggaaagt gtgacttgta ttttgtcggg ggaacaatta gctaaaaaaa tgagttttta
    40561 ctatggtaaa acttatgaca tagataaatg taataagtta ctatcactta tgacttttat
    40621 aggattgata aaaaaattag atgatgaaga tgttcctttg aagatgctcg aatacctaaa
    40681 taggttaaag gaagagaaag cgttaggaaa ctttaatcat aagagaagca atgtctatcg
    40741 attagacgtt tttaatgcaa cttctgtgat tcaaaaattg aatgaagata tccttccact
    40801 tctggaaaaa tttcatttta catatcaata tttttcttat gaatgggtta agatatgttt
    40861 taacgaaaaa gaggctaaaa gagtatttcc tcaaaattcc cgatcagaac ttagcaagga
    40921 aaaacaattg attttagatg aagcacatga agttttacag ggcttatcgt ggcaaggaac
    40981 atatctaatg acagagaaag aacttactaa cactgtttgt gaacgtctac gtaatgttgg
    41041 gaataacagc gtggaaaatg ctttaaaaga aaacagaggc tactttgtta aacaaggatt
    41101 cactcttgaa cgagcttcta aggatattaa gagactatac aatgtgaaag caagtagtag
    41161 ttttttagtc tacctagcaa aaggagcagg gatagatgaa gtaataaaag aattgatatt
    41221 atcaggagtt gaattagttc ctctttatca atttacaagt gtaacaagag atgatggacg
    41281 gagagtaagg aagagaaaag acccaattgt attaaactag acaggttatc catagctctt
    41341 aatcttattt catctcaata cacccaccgt taaagttcgg tgggttattt atttttcaat
    41401 ctttcaatag ctgattgcgt tcattttctt gcctttttaa tagcttcata tagtcaattg
    41461 ttcgaactgc atcttcagtg ctaagagaac gatagagagc caaaaactct tcctcataat
    41521 ctacgacaac attgtctaga aaatggctta caggggttcc tagggctcta gaaagagcgg
    41581 ttaagctatc gaggttaggc tctgttacag aattttccca tcttgctact gcctgttgtg
    41641 tagagaagat tttttcagct aattcttttt gtgtccaccc gagagctttt cgcttttctc
    41701 taatgacctt tgcatattgt ttctgtttca ttaatacacc cccatctcag tatagtacaa
    41761 caagttgttg tatttgtaat ggtatttgaa gtttttttat attttatgag ttgaaataca
    41821 tcatattgtt gtatataata aaacaggagg ggagaatcat gaaaaaagta agtaatctca
    41881 gccgatacag aaaagaaaaa ggactttctc aaacggagtt agcaaaaaaa tgaatgttac
    41941 acagcaatgt gtaagttcgt ggcaaacagg gagaacgatt cctaaaccct atcaaatgaa
    42001 gatgctttct gagatattaa gtgtaccaat gaatgagctg ttttctgatg tatttaataa
    42061 agtaaacagt taactaataa acaattgaat gtttttagga ggagaagaca tgtattgtag
    42121 aaaatgtggc aaagaaataa cagatgaaag taagtattgc cagttttgcg gagcactaca
    42181 agaaacagat gagacaactg gagcagacgc agtaaagaaa agtactgagc agacaattaa
    42241 tgaaatgaag gaacaaatcg tacatggaca agagacaggg aagctgtatt tgattattgg
    42301 ttggatttct atggtagtag cgttagcatt tattcctgtt gtatttggtg ctgttgctgt
    42361 tattatgggc tacttatatc gagataaaga tgaacaaatg ggtacgattt tgatgatagc
    42421 aggggtagct ggagcaatct ttggtgtact tttagggatg tcaatgtact gataagaggg
    42481 ataagagcaa attattttgg agataggaga taaaaagttg aataaagtaa atatgaacaa
    42541 gaagtttatc attatagtta tcgttgtctt ggctattata ggtatcattt tttcaaaaat
    42601 gacaggtaca tctggattag agaagtcatc gaaaccattg gtaacacaga ttttacaaga
    42661 acagtacaac ttaaatcgat catgtgatga tgtgacgatt actcatgaaa atggagataa
    42721 cacataccgg gctaaagcta tattagacaa tggaagtgct atcaacataa atattgagta
    42781 ctatccaaag aaagaccgta tatatgtgga gataccatat gctgaggtgt taatgttaaa
    42841 ttgatttaaa tgtattatgc ataccgtcca aacaggaact cccaaaggtg taaattcact
    42901 agggagtttt tatagtttcc tataaattaa atactaatac aggtgacaaa aaaatattac
    42961 aatgattgtc gggaattttt atgttattca ctgttataac aatataaaag aaatgaaaaa
    43021 gaaaaaatta ttatggatat gtttggtatt ttta
//
LOCUS       DAZUMT010000012        40890 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000012 DAZUMT010000000
VERSION     DAZUMT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.012"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..1352
                     /locus_tag="ACG8FM_000582"
     CDS             117..1352
                     /locus_tag="ACG8FM_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS5093271.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFAVLKRKINS
                     YQNPESHKTKLYCGAESFY"
     gene            1325..1405
                     /locus_tag="ACG8FM_000583"
     CDS             1325..1405
                     /locus_tag="ACG8FM_000583"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093272.1"
                     /translation="MLWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1531..2610)
                     /locus_tag="ACG8FM_000584"
     CDS             complement(1531..2610)
                     /locus_tag="ACG8FM_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5093273.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2713..3153)
                     /locus_tag="ACG8FM_000585"
     CDS             complement(2713..3153)
                     /locus_tag="ACG8FM_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5093274.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3210..3620)
                     /locus_tag="ACG8FM_000586"
     CDS             complement(3210..3620)
                     /locus_tag="ACG8FM_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5093275.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3800..4234)
                     /locus_tag="ACG8FM_000587"
     CDS             complement(3800..4234)
                     /locus_tag="ACG8FM_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5093276.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4334..5182)
                     /locus_tag="ACG8FM_000588"
     CDS             complement(4334..5182)
                     /locus_tag="ACG8FM_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5093277.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5571..7337
                     /locus_tag="ACG8FM_000589"
     CDS             5571..7337
                     /locus_tag="ACG8FM_000589"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS5093278.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7439..8698
                     /locus_tag="ACG8FM_000590"
     CDS             7439..8698
                     /locus_tag="ACG8FM_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5093279.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8845..10146
                     /locus_tag="ACG8FM_000591"
     CDS             8845..10146
                     /locus_tag="ACG8FM_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5093280.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10149..11000
                     /locus_tag="ACG8FM_000592"
     CDS             10149..11000
                     /locus_tag="ACG8FM_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5093281.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11013..11828
                     /locus_tag="ACG8FM_000593"
     CDS             11013..11828
                     /locus_tag="ACG8FM_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS5093282.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11979..13145
                     /locus_tag="ACG8FM_000594"
     CDS             11979..13145
                     /locus_tag="ACG8FM_000594"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5093283.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13375..14022
                     /locus_tag="ACG8FM_000595"
     CDS             13375..14022
                     /locus_tag="ACG8FM_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5093284.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14106..15050
                     /locus_tag="ACG8FM_000596"
     CDS             14106..15050
                     /locus_tag="ACG8FM_000596"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS5093285.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15185..16636
                     /locus_tag="ACG8FM_000597"
     CDS             15185..16636
                     /locus_tag="ACG8FM_000597"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS5093286.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16657..17493
                     /locus_tag="ACG8FM_000598"
     CDS             16657..17493
                     /locus_tag="ACG8FM_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5093287.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17493..18152
                     /locus_tag="ACG8FM_000599"
     CDS             17493..18152
                     /locus_tag="ACG8FM_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5093288.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18278..18967
                     /locus_tag="ACG8FM_000600"
     CDS             18278..18967
                     /locus_tag="ACG8FM_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093289.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18973..19929
                     /locus_tag="ACG8FM_000601"
     CDS             18973..19929
                     /locus_tag="ACG8FM_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS5093290.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19973..20485
                     /locus_tag="ACG8FM_000602"
     CDS             19973..20485
                     /locus_tag="ACG8FM_000602"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5093291.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20741..21289)
                     /locus_tag="ACG8FM_000603"
     CDS             complement(20741..21289)
                     /locus_tag="ACG8FM_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5093292.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21378..22559)
                     /locus_tag="ACG8FM_000604"
     CDS             complement(21378..22559)
                     /locus_tag="ACG8FM_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS5093293.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGLGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22795..24303)
                     /locus_tag="ACG8FM_000605"
     CDS             complement(22795..24303)
                     /locus_tag="ACG8FM_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS5093294.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24462..24653
                     /locus_tag="ACG8FM_000606"
     CDS             24462..24653
                     /locus_tag="ACG8FM_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093295.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24856..25773
                     /locus_tag="ACG8FM_000607"
     CDS             24856..25773
                     /locus_tag="ACG8FM_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS5093296.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25766..26839
                     /locus_tag="ACG8FM_000608"
     CDS             25766..26839
                     /locus_tag="ACG8FM_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093297.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26987..27505
                     /locus_tag="ACG8FM_000609"
     CDS             26987..27505
                     /locus_tag="ACG8FM_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093298.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27486..27878
                     /locus_tag="ACG8FM_000610"
     CDS             27486..27878
                     /locus_tag="ACG8FM_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS5093299.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27954..28889
                     /locus_tag="ACG8FM_000611"
     CDS             27954..28889
                     /locus_tag="ACG8FM_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5093300.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29022..29801
                     /locus_tag="ACG8FM_000612"
     CDS             29022..29801
                     /locus_tag="ACG8FM_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5093301.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29794..30111
                     /locus_tag="ACG8FM_000613"
     CDS             29794..30111
                     /locus_tag="ACG8FM_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5093302.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30355..31965
                     /locus_tag="ACG8FM_000614"
     CDS             30355..31965
                     /locus_tag="ACG8FM_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5093303.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32122..32970
                     /locus_tag="ACG8FM_000615"
     CDS             32122..32970
                     /locus_tag="ACG8FM_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5093304.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33201..33680
                     /locus_tag="ACG8FM_000616"
     CDS             33201..33680
                     /locus_tag="ACG8FM_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS5093305.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            33713..35941
                     /locus_tag="ACG8FM_000617"
     CDS             33713..35941
                     /locus_tag="ACG8FM_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5093306.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            36070..37713
                     /locus_tag="ACG8FM_000618"
     CDS             36070..37713
                     /locus_tag="ACG8FM_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5093307.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(37786..38331)
                     /locus_tag="ACG8FM_000619"
     CDS             complement(37786..38331)
                     /locus_tag="ACG8FM_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS5093308.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(38354..38839)
                     /locus_tag="ACG8FM_000620"
     CDS             complement(38354..38839)
                     /locus_tag="ACG8FM_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5093309.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(38829..39641)
                     /locus_tag="ACG8FM_000621"
     CDS             complement(38829..39641)
                     /locus_tag="ACG8FM_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS5093310.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(39780..>40890)
                     /locus_tag="ACG8FM_000622"
     CDS             complement(39780..>40890)
                     /locus_tag="ACG8FM_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS5093311.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13596 a         7419 c         7995 g        11880 t
ORIGIN      
        1 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
       61 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
      121 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
      181 agacagtcca cacagaggag atagaaacag ccaaatggac agaaaccaag tgggccaacg
      241 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
      301 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcaggagac ttagcaccta
      361 tactacaatc agctgttaca tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg
      421 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
      481 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
      541 atcatcattt aacgccgtat agtcagcggc agacaattga gcaagattca gctatcgact
      601 caggaggaga tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag
      661 taccgcctac agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac
      721 ccgatacaga aactcaacca gagccagaca atgaatcaaa acctgaaata acccctggag
      781 aaaaaccaga tgtagaccca gaagaaaaac cagatgtgac tccagaacca gatacggatt
      841 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
      901 aaaaaccaga ggtagcccca gaagaaaaac cagatgtaac tccagaacca gatacggatt
      961 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
     1021 aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg
     1081 caagagacca aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa
     1141 aagagagtaa aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa
     1201 aatcacgaat atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa
     1261 cctttgctgt cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa
     1321 agttatactg tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag
     1381 atttaaacaa aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat
     1441 aaaaagaaat atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc
     1501 gttcatactt tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg
     1561 gttcgggatc tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac
     1621 aatcaagccc caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa
     1681 aatcggcagt tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag
     1741 atggacaatc aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata
     1801 agctatgaaa gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa
     1861 aatgaaaatc atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat
     1921 tgtagtgagc aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa
     1981 tgtattgaca aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact
     2041 ttttaaaaac aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact
     2101 taagatgaat gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag
     2161 cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg
     2221 gatatattgt aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga
     2281 ctggatcatg tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata
     2341 gataaggtaa acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac
     2401 aaatcgctga atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa
     2461 aatggttgta atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt
     2521 gagacggcgt attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc
     2581 caaataacaa aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag
     2641 caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt
     2701 catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt
     2761 cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg
     2821 tggaatacct tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac
     2881 gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt
     2941 tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga
     3001 agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag
     3061 cggcttgagg aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa
     3121 acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag
     3181 tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct
     3241 atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct
     3301 tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag
     3361 ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata
     3421 tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca
     3481 aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat
     3541 tcgaatgtac gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc
     3601 gtagaataca tttttttcat aaaattacca cctttaatta tattttatac aattgaattt
     3661 tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc
     3721 acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa
     3781 aataatctca tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa
     3841 ttacttcacg atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg
     3901 agttagtcac accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc
     3961 gtaatgcttt attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat
     4021 ttggatgttc agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga
     4081 atctcgtacc atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct
     4141 caatcagttg tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat
     4201 ttaatacaaa atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga
     4261 acagttacta gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga
     4321 tttacttacc aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta
     4381 cgctgctgac aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg
     4441 aaaattcatg atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc
     4501 cttttgcctc tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt
     4561 cttccaaata atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga
     4621 tataagtcat gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta
     4681 ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg
     4741 aagtattcaa gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag
     4801 aagagttgtt tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga
     4861 cagacaacaa ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa
     4921 aaaccaagct tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta
     4981 gtaccaacat aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag
     5041 cttccagtaa tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa
     5101 acatattgat agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata
     5161 taaattgtct cgtttctttc atttattcac ttccttaatt cattaataat actcttatct
     5221 tattataact tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct
     5281 aaattattat gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt
     5341 ttactttaga aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac
     5401 aggaagtaga ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat
     5461 aaaaattcgg tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt
     5521 ctctatcact tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag
     5581 ctgcaattta tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc
     5641 gtattcggct tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc
     5701 catataccat aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa
     5761 gtaccaatgt tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt
     5821 atgcgttcca tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt
     5881 ttccttatcg agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc
     5941 atcaaatcga tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt
     6001 ttcccgaaag atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg
     6061 gaagtaaaga tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc
     6121 atttggatta cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag
     6181 ccacatccaa tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg
     6241 ataaaaaact tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt
     6301 tcgatgctgt attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa
     6361 acggagaaaa atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt
     6421 atcagatgac tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta
     6481 ctccattcat gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata
     6541 tcgcaactta ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg
     6601 aagtcgatca tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca
     6661 tctatatcct tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc
     6721 atgcagtgat gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa
     6781 tcacagcttc acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg
     6841 tgaacgaagg aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt
     6901 gccaaggtaa caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt
     6961 ctccttgtat ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc
     7021 gcaaatgtat gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag
     7081 aacttgtttc tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc
     7141 taatagtaaa tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca
     7201 tctatgttta ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga
     7261 ttcttagtca aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat
     7321 ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga
     7381 tttgtgccca ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat
     7441 gaaaaaaaga agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg
     7501 attagcggct tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac
     7561 gttaacagtt tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt
     7621 cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga
     7681 atcattagcg ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga
     7741 ccggatcggt gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc
     7801 agcttacgat gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc
     7861 tgcagtaatc gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc
     7921 aacgtttaaa gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc
     7981 aggaaaaaat acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat
     8041 tgcaggttac ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt
     8101 gaataatgca ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg
     8161 gccaaaggga atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc
     8221 caataaaact gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa
     8281 tatcaattat ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt
     8341 tggcggagga aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa
     8401 atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga
     8461 aatccctgca aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac
     8521 agctgtgatt gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga
     8581 agtttggaca ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa
     8641 agaatccgca gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt
     8701 aaaatgataa ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa
     8761 gagcgagatg gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt
     8821 agcaagttta gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa
     8881 aaaatcacgc tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt
     8941 aaaggtatcg tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga
     9001 tttcatgcat tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta
     9061 tttattttga ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac
     9121 ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc
     9181 aatcggacga tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg
     9241 acacttcccg cttatcttct catgattttt acgatcattt tcccagtcct agttacattg
     9301 ttcactgcat ttactaatta tgacttcaat catattccac cagccagttt aattgattgg
     9361 gtaggggcta aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt
     9421 gcagtctttt cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt
     9481 ggtattctca cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt
     9541 atcattttct tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat
     9601 atattcaacg atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt
     9661 ccttttgtag atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca
     9721 attatcttag tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc
     9781 ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg
     9841 cagcgctttt caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt
     9901 acacagtata cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt
     9961 cccggaagcg taggcggagg agctggttct actgatattc ttatttcctg gatctataaa
    10021 cttacgactg gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc
    10081 gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg
    10141 gattaacaat gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt
    10201 ttacttactt ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag
    10261 caagtactgc cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact
    10321 ggactttagg aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa
    10381 atacattgat catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg
    10441 gctatactta tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag
    10501 tgattcagat ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat
    10561 tagaagcgct cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa
    10621 tgaatacttg gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg
    10681 ctaaacttga tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca
    10741 aaccgatgct cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt
    10801 tagctaagtt cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat
    10861 ttatttctga tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg
    10921 cactaccgat ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat
    10981 ctggcggaac aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt
    11041 ccgcattacg tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat
    11101 ttttcttgat atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg
    11161 ccaatcagat ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg
    11221 aacaaggaac ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc
    11281 aacaaacgat agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac
    11341 aatccgatca tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg
    11401 ataaatctgg tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg
    11461 tacgtacgcc cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc
    11521 caacaatcat tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc
    11581 tcattttatt cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca
    11641 ttcatacatt caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg
    11701 cagcagcagt tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa
    11761 cgataactgt cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg
    11821 tcgaatgaca agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag
    11881 tagtttttga aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt
    11941 attaacgtaa tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca
    12001 atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa
    12061 cgaaacaata cggagatacg gacactcctc cccaaatcaa aatcaatatc catgaagaaa
    12121 tcccaatgaa agagattaca agtaacgctt ctttggcact aggagaagcc tatatggatc
    12181 atcgaatcga gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa
    12241 acagcttttt acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga
    12301 aacaaaaaaa caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac
    12361 tttggctaga tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt
    12421 tggaacaagc acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag
    12481 gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag
    12541 agtataatgt caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca
    12601 aaagaataaa agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg
    12661 aactaaaagg cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt
    12721 ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag
    12781 ctttgatcca tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat
    12841 atattttccc tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa
    12901 acgatttgca actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac
    12961 attggacaaa gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct
    13021 tctatcgaat gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata
    13081 tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg
    13141 cataattgag gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg
    13201 ctccagaagt gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt
    13261 tctttatatt ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt
    13321 ttcttcgtta tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa
    13381 aaaactgtta tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat
    13441 accaatcaag gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta
    13501 tcggactcct caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa
    13561 gcaagtactc agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat
    13621 aaatacccag atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc
    13681 tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat
    13741 gaaaaaatgg agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag
    13801 aaaaaagaag atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc
    13861 gccgtcgaca aagtaggaag cggtacggca acagattggg atttggataa agatttaggt
    13921 actacttatt gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat
    13981 agccaaacag gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca
    14041 agagttgatt tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga
    14101 tttacttgag tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg
    14161 catctggtgt ccactgcatg actacccagg aattagatga actggctcat tcagaagcag
    14221 gagcatttat cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct
    14281 attttgatgt tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt
    14341 attatctaga gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat
    14401 ttttttccat tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa
    14461 aaagtgggtt taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac
    14521 cacaactagc ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct
    14581 tttctaagcc gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga
    14641 tggctgatat cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca
    14701 atggtttgta tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg
    14761 gcggcattgg cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata
    14821 cacgtttgaa accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg
    14881 catttgaaca tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag
    14941 aagggccgga aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag
    15001 gatatacgtc aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg
    15061 aaggtcatga ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc
    15121 aagcgtactc attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga
    15181 taatatgaat gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca
    15241 agaactagtt acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt
    15301 tgtcgcgctt gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa
    15361 agggccattg gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg
    15421 gctagctact tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta
    15481 tgtgcggcaa gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt
    15541 tggtttcaaa aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct
    15601 tgaatgtaca ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt
    15661 tcctttagct ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg
    15721 attgattggg ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg
    15781 atggcaagga gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt
    15841 attttatggg atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg
    15901 gcaaacacat ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca
    15961 ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa
    16021 agaagggcat gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc
    16081 ttattatcag atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat
    16141 ccagcgagct gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg
    16201 tcagaagctc tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat
    16261 tgtcatggaa aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac
    16321 cgcccctaaa atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat
    16381 cagtgattgt tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt
    16441 gaagatcaca ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc
    16501 tactcatttg acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag
    16561 agaagatttg ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc
    16621 aaatccatgg aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct
    16681 ctaataacta gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa
    16741 gataacagct ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca
    16801 ctgtttcccg catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg
    16861 gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat
    16921 ggacaaatca cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc
    16981 acaaaagaaa gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt
    17041 atgatcgtac gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa
    17101 aaagcagctg gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa
    17161 ttagtcacat atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga
    17221 acagatgtta ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat
    17281 atccctgtaa aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg
    17341 aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa
    17401 gatgggacga caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg
    17461 aaagaaacga tggctgagtc ggtgagtgag tgatgtactt tcctctaatt gatttttccc
    17521 tcatgtggac ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct
    17581 tcgtgtcgtt tcttttagga aatctggtag ggttgctttt gacagtttta ggtcttttgg
    17641 attggctgcc tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg
    17701 cactagtcct gctgtttctg ttatattttg ggttacctta tcaattaagt gccttaacgg
    17761 cttcagtcat ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat
    17821 cactagcagg cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct
    17881 ttggggtatt gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca
    17941 gtaacgtagc tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag
    18001 aaatatttca aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata
    18061 ttttagttgc actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt
    18121 tagaaaaacg ttctatgcat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg
    18181 caccatttct attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt
    18241 aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga
    18301 aatagaatcc tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt
    18361 cacgatgcct gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg
    18421 aagccgagaa tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc
    18481 caaactgtca tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga
    18541 agtaaaaact gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga
    18601 agaggagatg cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct
    18661 tgctgtaccg gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag
    18721 aaaagcaaag caaatggagc gaggcgtaaa atattttcca acagatcaat tgtacaaaga
    18781 attgaaaaga cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt
    18841 tttagaggaa atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca
    18901 gcatgattat gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt
    18961 ggagtgaaaa atatgtacta tccttattta agaggaaaac aatttgacct attagcatta
    19021 aaagaagcac tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta
    19081 cgtgattccg ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatccaatc
    19141 gctgtgatcc aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt
    19201 tggacagtca aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc
    19261 agtgaagtac tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa
    19321 gatacagagg tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact
    19381 agtcgctttc gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg
    19441 agaaagcatg ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt
    19501 tttttccacg cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat
    19561 ttcgataaag ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac
    19621 ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca
    19681 ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa
    19741 ctcttgatta cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct
    19801 ggactgggag tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg
    19861 ctagatcatc caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg
    19921 atccaatgat tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat
    19981 tcggcaaaca aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga
    20041 tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac
    20101 gattttgaac gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc
    20161 tggaacagct gcattagtct caagcgtgga tcccgtttac acaaaaatag atggtagttg
    20221 gaaagaatca actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg
    20281 aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa
    20341 tatccaagat atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta
    20401 ttctgctgga ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa
    20461 agcatatcaa ataatcatca attgactata aaatcatctg agcaaagata aagacattaa
    20521 cactactgaa aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc
    20581 aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt
    20641 ttacgaaata gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc
    20701 acgacctctc ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt
    20761 ttattttatc tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga
    20821 tagctttcaa acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa
    20881 tctgttcaga tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg
    20941 taataaagat aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg
    21001 ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg
    21061 aaccatattt atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct
    21121 gattgaatgt attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg
    21181 gtgatcgatt cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg
    21241 aagtttttcc agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc
    21301 ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa
    21361 ctttgctttt ttgtatatta taccagcaag ccagtcacaa tagcacttaa aaagctgact
    21421 aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca
    21481 tttaatccct tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg
    21541 aggaagacag atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat
    21601 tctgtcatag caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc
    21661 actgcttctt tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg
    21721 atctcttgga aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa
    21781 gcaacaaaac cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg
    21841 tattctccca acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct
    21901 tctttttctt ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag
    21961 ttcaaaacaa gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg
    22021 atcgacatag aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat
    22081 aggaagggta attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa
    22141 gctactccat tataagattc caatttgccc aacccgttga ttttacttaa tcctagacca
    22201 acagctttca taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa
    22261 ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca
    22321 ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt
    22381 gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca
    22441 agctgtaaaa cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg
    22501 atcaaaaaag caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa
    22561 taatcactcc tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc
    22621 tttagtgaaa ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata
    22681 aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg
    22741 aaactatcga ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg
    22801 gtaatataga ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt
    22861 caatgtttgt tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag
    22921 ttcagctagt ttatcataag caacttgagc gattggattc gttactgcac tgattgataa
    22981 agcgatcaag acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt
    23041 caatccttgt attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg
    23101 ggctaaatgt tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt
    23161 acgcccggta atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt
    23221 tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg
    23281 gtcttcagga cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc
    23341 gtcgatcaat ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg
    23401 tttagaggct tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc
    23461 catgtcagta ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt
    23521 gattacagga aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc
    23581 aaagtggaag gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc
    23641 aatgttcagc ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta
    23701 accagctgca tgtccttttt ggctttcatg atacagttgg ttcaaggaag tagcgagctt
    23761 tccacttcct gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc
    23821 attttttcca aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat
    23881 ttctgagtgt ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt
    23941 ggcaggctgt ccactatagc gagtgataac gacactgttg attaataagc cataaccacg
    24001 taattcatcg attaaccgca agatatccat atcataagta atcccataat caccacggat
    24061 cttattacgt tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa
    24121 tttttgcagc aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg
    24181 cttgtcgtca actagcttcc caccaaattc taaatataat ttgtcatagt ggttcactcg
    24241 ttccaaaata tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt
    24301 cactaggttc cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact
    24361 ggaagagcga aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag
    24421 ttattcattg gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa
    24481 agaaaaacgc atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg
    24541 aagatcccga attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc
    24601 gtgatgagca tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa
    24661 gataaaggtc atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa
    24721 aaggctgtac caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata
    24781 acaatttttt acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa
    24841 gaggaggcaa gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta
    24901 gaggagacga atgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa
    24961 gaaaaagcaa gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct
    25021 gtccgcgaag aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa
    25081 ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat
    25141 gatcgttttt tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg
    25201 agctattaca aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt
    25261 ctgccagaaa tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg
    25321 gagtcaacgg actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag
    25381 acagaaattg aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg
    25441 aacgacaata aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag
    25501 aagaagaagc aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct
    25561 gaaacaatcc agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt
    25621 gcagccatcg tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga
    25681 ttcggtcaag caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat
    25741 caatctaatg aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa
    25801 tgaagaacaa acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa
    25861 tcctctggat gacacagaag aaatccctat gctagaggaa acaaaaatac acgaaaaaga
    25921 aaaagtaact ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga
    25981 aaatcccggg tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga
    26041 aacagcggaa gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt
    26101 tccacaatta ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat
    26161 cggaaagaca agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat
    26221 tggtctggaa cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa
    26281 ttatacgttg aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca
    26341 acgataccaa gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa
    26401 tgtccaagat gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc
    26461 tgcagggcag atcacgatat tcaatcacaa attgaatcaa ttattagccc atgcaagaac
    26521 acttggtttt accgttaatc agacattact agacaatacg aagccacttc gtttgaatct
    26581 tcagagcgaa tttgaattga cgaatgaagt attggatgat atttttatcg tggcaatgaa
    26641 aaatccatcc tacgatatgg aaaagatcaa cgaaactact tatcaagtac gattagataa
    26701 aggacagtct ttaacgataa caacaaacga aaaagatcaa acagtcttgt cgatctcatc
    26761 tggagactac ccacgatctg tcattgattt ctttatcaat tacgaattct tggtcccatt
    26821 aatggtgaga aagtcctgat tggataagtt ggattaccag atgtcattca gataattctg
    26881 ttccaaagga cttgcggcag ttatccagaa taattgtcac aagtcttttc tgtatgtact
    26941 aaatggacgt gaataacttg ctgatgaagg agggatttca ttaggaatgt ccacaaataa
    27001 aatagcacag cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt
    27061 tattccgaat ttccagccaa tgactgctat ttttttattt atagtacttt acttaagctt
    27121 aaaagatgcg ttaattgtca tgagtctttc tattgcgatc agcagttttt attttggtgt
    27181 tggaccatgg gtcatcggcc aatggatcag ctatactgtt gttctgagtt tgttttcaat
    27241 cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta aagggagtct gtttttttct
    27301 ggcgggcatg gtttacggaa taggtatgac cgtttttgat acgctgttat atcgtttgcc
    27361 tcaaccttgg atctattacc ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa
    27421 tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc attcaggagg
    27481 aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag tgtcagtata ctcattttga
    27541 caagttgcag tacaacctat acacaatcgg cttccaacca aaaggaggaa gtaaaaacat
    27601 cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt
    27661 tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg aaattttgaa gtcaaagaag
    27721 ataacggctt catcacttcg cttgaagggc actcacagga tgagaaaaat caactctatt
    27781 ggatgtttac tgtggacggg aaaagtgcta ctactggtgc caaagagatc aaattgaatg
    27841 atggacaatc ggtcattttc aatttgtcta aactataagt ttcataaaaa actgttaaag
    27901 ggcgaatatt gccataattc agtgagttca gttgatgaaa gaaggaaaat aacatggaaa
    27961 attttaaaaa aagcggaatg ttttccgtaa ttgctgctct aggggctaat attctagttg
    28021 ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc cgccatgcta aacgaaagca
    28081 tccacagtat cgttgattgc agcaatcaga ttttgctgtt attcggtgat aaacaagcca
    28141 ataaaggaca gagcgagttg catcaatttg gtgaaggacg agcaaaatac tttttcagta
    28201 caatcgtcgc aatgatgctt ttcttcggcg gtggggcact tggggtgatg gaagcttttg
    28261 aaaagttgac gcatccatcc catgaagtgg gtaatccgat cattgttata gcaatactgt
    28321 tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat
    28381 tgaatactga aaaactttcc acgatgcgct ttttgcgcga aagtcgacac agtgaaatac
    28441 tgattatttt tacggaagac ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa
    28501 caatattgac ttatgcaacc gggattgctg catttgatgc gatcagcgga ttgctgatcg
    28561 gtttcttgct gatgggagcc gccattttcc tagcaaaaga attttatagt ctgatcatcg
    28621 gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga
    28681 tcagtcggct gatcgatata aaaacagttc atttaagtcc aacggagatt ttagttgcag
    28741 caaaagcaga tatcgttggt aacaaagaga atgaagctta ctcggtcatc aatgatattg
    28801 aaaaaaatat gcgtcagtct cttcctgaca agaagatgta tatatacatt gaaacagata
    28861 aatatgaccc aaattattct cgtaaataaa acgtttattc atgaattgaa gcaaaaatga
    28921 gaaaaatata atcttgtttt taaataaaac ggctacaatt tagcgaattt atgttacaat
    28981 gagtccgctg tttatttgtt gtaattaaag gagatatttt aatgaacaaa aagaaaagtg
    29041 cgccatggct tcaagctttg catgcagtga tgccactttg tataagctat ataccagtag
    29101 gattagcttg cggagtattg ttgcaaaaag ttggatttga tcctctctta tcaggactgt
    29161 tatcgattct ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg cttacgacgc
    29221 aagcatcatt tgcaacgaca ttattgatgg ttttcttttt agaattacgc tatacattat
    29281 taggagcaag tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt
    29341 ctcaatcact gaatgatgaa aactatgcgg tgaactactt aaagttttca actgaccctt
    29401 cctggaataa aagaaaggct ttatatgtca attggttctc aatgactgca tgggccggta
    29461 gcaatatgat cggtaatttt ttcggctcgg tgattcgagt agatgctgac cttgtccatt
    29521 ttgctttgac ggccatgttc attttcatgt ttatcatgca gatgaaaagc gcactactga
    29581 tctttacagg tttattctca ggcgtattag gggtcgtatt catggtatta ttccaaaata
    29641 ctttcggatt gattgcagca acagtcattg cttcatttgc cggatttatg ctggagaagg
    29701 cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg taaagatgtc aatgagccgc
    29761 tcttcaactt cccggaacag gaggccaatc accatgacta aactatattt gctactttta
    29821 gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct ttattttact
    29881 catcgaaaag tgccatcctg gttcagtgac tggatgaaat atgtaccggt ggcattattt
    29941 gcggcgttag cttttaaaga tgttttcatt actcatgaac atctggatat tgcttggaat
    30001 atcaaaatag ctgcaatgat tctagtagca ggcgtggctt acaaaacacg ttcgatggcg
    30061 ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt ctatgcttta aactgtttta
    30121 ttttattaaa aacagagatt gcgacataag ttgttcaaac tgatctatcc gaactgcatt
    30181 tgcaacagtc aattctggat gcaagtttat tcgtagggat aaaaaagatt agtgttggct
    30241 tatgatgcaa gctctttttt attttaggaa ttagagaggg atttgtcctt ctatgatttt
    30301 ttgctataat agggaagatg tttattttat gaaagaatag ttgagggaat ttaaatgaat
    30361 aaaaaattga tgcaaggaac attttggcta acctttgcta atcttctatg taaagtttta
    30421 ggggtagtct atttgattcc ctggctaagt atgatgggga acaatcaaga tggtatgctt
    30481 gctacgacat tgtataatgt tggctatctt ccttatggat tgttcctgat gcttggtaca
    30541 gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac
    30601 gctgcttgtc ggctgatttt tagaagtacg atcaatatca tgtttgtcat cggtatcgcc
    30661 tctgctgcac tgatgtattt atttgctcct ttactagccg gaatcagtcc gattgctaac
    30721 gttaataatg ggatactagc tatcagaagt ttatgcccat ctttgattgc tatcccaatt
    30781 cttagtgcaa tgagaggata tttccaagga aaaaatgatc tgcggcctta tggaacttct
    30841 ttgattatcg aacaggctgt gcgagtgatc gttatcttgg ctggtactta ttacttgcgc
    30901 gtattgactg acggaaccat attagaagct gtattgatca gtacagtcgc ttctttcttc
    30961 ggtgggttag ctgcgattat ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa
    31021 cttaaagatt ttttgatttc cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc
    31081 tctatcattc gggaaacact accgtttatt tttgtcggct cagtcattac tatcgttcaa
    31141 atgatcgacc aagtaacgat gaaaccactg cttcactttt tccgtccaga gatcgcagat
    31201 cagcaactgg aattcttatt cagccgtgct tctgtcaatc caaataaatt gacattgatc
    31261 ctgatctcca tggtaggaac agtagctata agcagcttgc caatattaag cacgatgaag
    31321 aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata gtttttcgat tgcgttattg
    31381 attcttcttc cttcattgac tggaatgtct ttattagctg gaccactata cacattgttt
    31441 tttggctatg atcctgaaag tgtcggatat ttccaaatgg ctttgctagc ttctttgttt
    31501 ttctctttat tcacgatcct atctacgatg cttcaatctt tgaatcacca tcgttttgcg
    31561 atccgattaa ctgtagaagc aattatcctg aaagtcatct tccaagtaat cggattagga
    31621 ttagttggcg gatacgggat gagcttatca aatggcttag cattcggtat tgtctttgtt
    31681 cgcggctatc actttatgtg caaagaatac cgaatagcac ctttagcaaa gatcagcaat
    31741 tttttcttaa aaactttccg ctccaccctg attatgcttg cagtatgttt tgccgttttc
    31801 tttttgctga atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg
    31861 gctgtaggaa gtattggcgg cttagtattt ttattttctc aattcggaaa aaatgggttt
    31921 cgaatgttga aaaatgttaa acatcatagc agaagcaaag aataggttaa ctaatcgttt
    31981 tttgctgtca cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt tctattttcc
    32041 aattttagtt tcatatctaa tcttacttat gatatagtta taaggtctgg aaaaattctt
    32101 tgaaacgagg aaattaaaca catgaaactt gcagtagtta cagatagttc ggcatatctg
    32161 ccggaacgta tcaagaacca tcccgactta ttcgttattc caatcccttt gattatggat
    32221 gggaaaattt ataacgaagg aatcgatatc gaagcggatg aatattatgg acttttaaaa
    32281 aacagtaaag attttcctac tacttcacaa cctgtcgtag gggaagtttt gggtttatat
    32341 gaggaattga aagagaaagg gtatgacacg attttaagta tccatctttc ttcagggatt
    32401 tctggctttg tcaacacatt atttgcgatg aaagatgaca tcagcggagt aacggtcatt
    32461 ccttatgact ctaagatcac tagtatgccg atgggacaca tggtagaagc tgccttagac
    32521 ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac atatcgatcg aatccgagat
    32581 aatacctacg cttatctgat tgtagatgat ttgaacaatt tagtacgagg aggacgtttg
    32641 acaaatggtg cagcgctgat tggcagctta ttaaaaatca aaccgatcct gacattttct
    32701 gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct
    32761 gaagaaatta ttggtagaag aaataacgaa atacaaccgc cagtcaaact ttacgtgata
    32821 catgcaaata atttagaagt agcgattgcg gagaaagaga aattgcagaa aaaatatcca
    32881 aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg gtacacattt aggtgaaaaa
    32941 gccatcggca ttgccatttc agcgcaataa ttccttttat tcaaaaaaca taccaaaaat
    33001 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
    33061 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
    33121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
    33181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
    33241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatacggat gcaaaagggc
    33301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
    33361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
    33421 tagatttagc gatcttagct gcacaaggaa gtttatactt ccaagatgct tatcgttatg
    33481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
    33541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
    33601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
    33661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
    33721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
    33781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
    33841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
    33901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
    33961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
    34021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
    34081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
    34141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
    34201 cccttctgaa atcgttcgag cgattgaaaa aggatattta ttagagattc aagagccgac
    34261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
    34321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
    34381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
    34441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
    34501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
    34561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
    34621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
    34681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
    34741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
    34801 tacaaaagga agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
    34861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
    34921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
    34981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
    35041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
    35101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
    35161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
    35221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
    35281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
    35341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
    35401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
    35461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
    35521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
    35581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
    35641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
    35701 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
    35761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
    35821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
    35881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
    35941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
    36001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
    36061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
    36121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
    36181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
    36241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
    36301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
    36361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
    36421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
    36481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
    36541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
    36601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
    36661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
    36721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
    36781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
    36841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
    36901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacttgca
    36961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
    37021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
    37081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
    37141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
    37201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
    37261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
    37321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
    37381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
    37441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
    37501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
    37561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
    37621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
    37681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
    37741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
    37801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
    37861 tttcaaacga ggtctgacag gtacttggac atgtttgata tgcataccga ttgatgtgtc
    37921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
    37981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
    38041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
    38101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
    38161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
    38221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
    38281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
    38341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
    38401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
    38461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
    38521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
    38581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
    38641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
    38701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
    38761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
    38821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
    38881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
    38941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
    39001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
    39061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
    39121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
    39181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
    39241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
    39301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
    39361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
    39421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
    39481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
    39541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
    39601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
    39661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
    39721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
    39781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
    39841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
    39901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
    39961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
    40021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
    40081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
    40141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
    40201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
    40261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
    40321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
    40381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
    40441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
    40501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
    40561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
    40621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
    40681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
    40741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
    40801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
    40861 gcaggttgtt ccattaaata aagaatagga
//
LOCUS       DAZUMT010000013        39783 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000013 DAZUMT010000000
VERSION     DAZUMT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.013"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..574)
                     /locus_tag="ACG8FM_000623"
     CDS             complement(29..574)
                     /locus_tag="ACG8FM_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5093312.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="ACG8FM_000624"
     CDS             complement(586..1278)
                     /locus_tag="ACG8FM_000624"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS5093313.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACG8FM_000625"
     CDS             complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACG8FM_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS5093314.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="ACG8FM_000626"
     CDS             complement(1594..2157)
                     /locus_tag="ACG8FM_000626"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5093315.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="ACG8FM_000627"
     CDS             2385..3047
                     /locus_tag="ACG8FM_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS5093316.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="ACG8FM_000628"
     CDS             3171..4367
                     /locus_tag="ACG8FM_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS5093317.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="ACG8FM_000629"
     CDS             complement(4468..5844)
                     /locus_tag="ACG8FM_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5093318.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6747)
                     /locus_tag="ACG8FM_000630"
     CDS             complement(5881..6747)
                     /locus_tag="ACG8FM_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5093319.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6836..7861)
                     /locus_tag="ACG8FM_000631"
     CDS             complement(6836..7861)
                     /locus_tag="ACG8FM_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5093320.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7879..8553)
                     /locus_tag="ACG8FM_000632"
     CDS             complement(7879..8553)
                     /locus_tag="ACG8FM_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5093321.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8737..9630)
                     /locus_tag="ACG8FM_000633"
     CDS             complement(8737..9630)
                     /locus_tag="ACG8FM_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5093322.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9704..11401)
                     /locus_tag="ACG8FM_000634"
     CDS             complement(9704..11401)
                     /locus_tag="ACG8FM_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5093323.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11394..12707)
                     /locus_tag="ACG8FM_000635"
     CDS             complement(11394..12707)
                     /locus_tag="ACG8FM_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5093324.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12707..16648)
                     /locus_tag="ACG8FM_000636"
     CDS             complement(12707..16648)
                     /locus_tag="ACG8FM_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5093325.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16757..17215)
                     /locus_tag="ACG8FM_000637"
     CDS             complement(16757..17215)
                     /locus_tag="ACG8FM_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5093326.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17513..18031)
                     /locus_tag="ACG8FM_000638"
     CDS             complement(17513..18031)
                     /locus_tag="ACG8FM_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5093327.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18033..18650)
                     /locus_tag="ACG8FM_000639"
     CDS             complement(18033..18650)
                     /locus_tag="ACG8FM_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5093328.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18647..19501)
                     /locus_tag="ACG8FM_000640"
     CDS             complement(18647..19501)
                     /locus_tag="ACG8FM_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5093329.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19498..20409)
                     /locus_tag="ACG8FM_000641"
     CDS             complement(19498..20409)
                     /locus_tag="ACG8FM_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5093330.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20450..21784)
                     /locus_tag="ACG8FM_000642"
     CDS             complement(20450..21784)
                     /locus_tag="ACG8FM_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5093331.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21992..22822
                     /locus_tag="ACG8FM_000643"
     CDS             21992..22822
                     /locus_tag="ACG8FM_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS5093332.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23066..23315)
                     /locus_tag="ACG8FM_000644"
                     /pseudo
     CDS             complement(23066..23315)
                     /locus_tag="ACG8FM_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23506..23700)
                     /locus_tag="ACG8FM_000645"
     CDS             complement(23506..23700)
                     /locus_tag="ACG8FM_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093333.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24205..24390)
                     /locus_tag="ACG8FM_000646"
     CDS             complement(24205..24390)
                     /locus_tag="ACG8FM_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093334.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25240..25581)
                     /locus_tag="ACG8FM_000647"
     CDS             complement(25240..25581)
                     /locus_tag="ACG8FM_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5093335.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25581..25811)
                     /locus_tag="ACG8FM_000648"
     CDS             complement(25581..25811)
                     /locus_tag="ACG8FM_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5093336.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26098..26436
                     /locus_tag="ACG8FM_000649"
     CDS             26098..26436
                     /locus_tag="ACG8FM_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093337.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26641..27203)
                     /locus_tag="ACG8FM_000650"
                     /pseudo
     CDS             complement(26641..27203)
                     /locus_tag="ACG8FM_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27935..28516)
                     /locus_tag="ACG8FM_000651"
     CDS             complement(27935..28516)
                     /locus_tag="ACG8FM_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5093338.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28699..29325)
                     /locus_tag="ACG8FM_000652"
     CDS             complement(28699..29325)
                     /locus_tag="ACG8FM_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5093339.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29347..30678)
                     /locus_tag="ACG8FM_000653"
     CDS             complement(29347..30678)
                     /locus_tag="ACG8FM_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS5093340.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30833..31234)
                     /locus_tag="ACG8FM_000654"
     CDS             complement(30833..31234)
                     /locus_tag="ACG8FM_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5093341.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31320..31526)
                     /locus_tag="ACG8FM_000655"
     CDS             complement(31320..31526)
                     /locus_tag="ACG8FM_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093342.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31621..33123)
                     /locus_tag="ACG8FM_000656"
     CDS             complement(31621..33123)
                     /locus_tag="ACG8FM_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS5093343.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33141..33596)
                     /gene="argR"
                     /locus_tag="ACG8FM_000657"
     CDS             complement(33141..33596)
                     /gene="argR"
                     /locus_tag="ACG8FM_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5093344.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33694..34311)
                     /locus_tag="ACG8FM_000658"
     CDS             complement(33694..34311)
                     /locus_tag="ACG8FM_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5093345.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34532..35905
                     /locus_tag="ACG8FM_000659"
     CDS             34532..35905
                     /locus_tag="ACG8FM_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5093346.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35980..37092
                     /locus_tag="ACG8FM_000660"
     CDS             35980..37092
                     /locus_tag="ACG8FM_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5093347.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37164..38801)
                     /locus_tag="ACG8FM_000661"
     CDS             complement(37164..38801)
                     /locus_tag="ACG8FM_000661"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS5093348.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38969..39577)
                     /locus_tag="ACG8FM_000662"
     CDS             complement(38969..39577)
                     /locus_tag="ACG8FM_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5093349.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11436 a         8452 c         6901 g        12994 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggaa tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaaaatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac
     5941 aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa
     6001 agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt
     6061 gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt
     6121 ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat
     6181 tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat
     6241 ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc
     6301 aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc
     6361 accgcttaca agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca
     6421 ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa
     6481 tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac
     6541 actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact
     6601 aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg
     6661 ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat
     6721 agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa
     6781 cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac
     6841 gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt
     6901 cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa
     6961 gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat
     7021 caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc
     7081 tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt
     7141 tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa
     7201 cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct
     7261 gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc
     7321 gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg
     7381 ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa
     7441 ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat
     7501 ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca
     7561 gctgattgat atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat
     7621 tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca
     7681 agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa
     7741 ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa
     7801 taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca
     7861 tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata
     7921 tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt
     7981 attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa
     8041 gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc
     8101 aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg
     8161 gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat
     8221 ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt
     8281 catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt
     8341 cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc
     8401 tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt
     8461 cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat
     8521 ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc
     8581 tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt
     8641 agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag
     8701 cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg
     8761 tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat
     8821 acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga
     8881 agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac
     8941 ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt
     9001 atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc
     9061 atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt
     9121 tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg
     9181 aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc
     9241 aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc
     9301 gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac
     9361 ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac
     9421 tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc
     9481 gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac
     9541 tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt
     9601 ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc
     9661 agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca
     9721 ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga
     9781 atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc
     9841 tttctggccc tggattttct actcctacga tctgaatttc ttcattatag cttccataag
     9901 agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca
     9961 tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc
    10021 catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg
    10081 attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt
    10141 cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt
    10201 caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa
    10261 attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta
    10321 gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta
    10381 atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa
    10441 acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg
    10501 ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt
    10561 tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag
    10621 gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt
    10681 cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca
    10741 tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg
    10801 tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca
    10861 tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga
    10921 aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata
    10981 ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa
    11041 accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt
    11101 cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt
    11161 caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc
    11221 cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca
    11281 cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc
    11341 ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca
    11401 tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct
    11461 agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat
    11521 tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc
    11581 tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct
    11641 aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc
    11701 caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc
    11761 acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta
    11821 acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg
    11881 tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc taaataatat
    11941 ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt
    12001 tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca
    12061 tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg gtgataaatt
    12121 ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct
    12181 ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt
    12241 gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc
    12301 gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc
    12361 gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca
    12421 tgataataat cggtcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt
    12481 gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg
    12541 tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc
    12601 acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc
    12661 ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct
    12721 cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg
    12781 cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag
    12841 gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta
    12901 actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt
    12961 ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc
    13021 gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca
    13081 cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt
    13141 ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct
    13201 caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt
    13261 ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt
    13321 catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact
    13381 taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg
    13441 cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta
    13501 aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac
    13561 gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag
    13621 aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta
    13681 attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata
    13741 aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct
    13801 tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat
    13861 caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg
    13921 tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt
    13981 tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc
    14041 ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt
    14101 ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac
    14161 tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca
    14221 gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa
    14281 gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc
    14341 tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg
    14401 ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa cgtggtggtc
    14461 cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg
    14521 gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt
    14581 cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct
    14641 cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg
    14701 aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct
    14761 gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt
    14821 ttacttggta cttgatcctg accatttccc cttcacctaa agaaatcgga gaaaatttat
    14881 aagcttggtt ttcccgtccg acatacgttg tcggagtcgc cactacttct ggtggggctt
    14941 gtccatgacg ttcttcatcc catatttctg gctcttggta cttcatgccg ttttctcctg
    15001 gatctagatg gtaaaactga atatctgccg atctgccagc tccaaatggt gagacattaa
    15061 aaccagtttc atcataaagt gtggcttgct ggatcgttct cttgaaggta gaagagatat
    15121 cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc
    15181 catcttcttc taaatcagca atatttttca atacatttcg tccaatagca tctagtcgtt
    15241 gtctcccgct aacgtttcca tcgattccta aaccaatgga gaagatactt ggcgggttct
    15301 cttgatactt gtttttcagc caatgatgat aagcaactgt gttgatcgca gatgactttt
    15361 cttgcgaagt aggactgcca ttcgtattgt tattagaaga accatcacca acccagtgag
    15421 tcgctttatt atctccttct tttacctctc cataacgata gtgtacattt acttcatctt
    15481 ctggacggtt aaagtcagat gcataggtaa ctggatgagt aaaccctgca ttagaagtat
    15541 tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca atcttatctt
    15601 ccatcacagt ccagttgctc caagctccat tagtgctcgt acgataactt gactgaatcg
    15661 gtgcataact gaaggtcggt gtcccatctc ccacaacgat taggattttt tctggatttc
    15721 tattttctcc gccattgtct ttatacaatg tttcatagcc atttttaagc cctagtgtaa
    15781 gcggagttcc tccgattggt gtacgattta atgctgtatt ctcacttccc gtcaaacgat
    15841 tgatatcatt tgtcaatgga aagatgtccg aactgatttg ctcatggtta ttgatagctg
    15901 tactattatg gtaaaaattc accataccga ttcgaatatc cagactcggg tcagagagca
    15961 gatctttact catttcgttt actgcctcaa tcaacgcagc atttttagtt tgactattgg
    16021 tcccagcggt taattcactc atactcgctg acttatctag aacaaaaacg atatcgattg
    16081 gactgatttc ctttttctcg cccccgatga catctagata gacattgtaa agggttgttg
    16141 catcttcagg atgtagatca gatgctgtgc tgcttggcag aacaggttct acccattttt
    16201 tgatgtacgc cccgttataa tccaagtaac tgttttcaaa attccgttca tttccccaaa
    16261 gctgaacggt ttgagctgga acgtttctat cttcttcgcc tgcagttgat ccattattag
    16321 tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt ataatcacta tcatcgatca
    16381 agttctctcc gtcatatggc gagcctttgt ctccttgata gcgttcattg ctttgtgtcc
    16441 aatttcctaa cacttttgta tagtcattcg ttgtaaactt cgggtagcga tgctgttcgt
    16501 tttcaacttc tccgctagga tagatatatt cataggggtc ttgcgtagct gttggcccaa
    16561 gacgctccaa aataccgttg aataactggc gttgaatgcg ctggagtgct tgaccgctgg
    16621 gtacgattgg ttcgtccagc ttttccatct cattttcttc gctacgttga atttcagcgg
    16681 aatcttctgt atcagtttca ttttcttgta attcttggtt atctattctt tcatcaacca
    16741 ccacttgaag aggtagttac ccttcgttca catcggggat cttttctgtt tcaaaaatca
    16801 atgtgccggt ttctgcttct tttgaataaa cttttccgac ataccattct tgaccatcga
    16861 tcgtctcgac ttttaggtct tctccatctt catcatagga ggatagacct gttccacgaa
    16921 cgttttttac ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt attctacctt
    16981 cattggcact ttcctttttt tcaaaagcca gatgccattc aagggtttct tctttttcaa
    17041 tatacttccc ttcaattttt gcatagtcct catctaagaa tacctcatcc tcctttgttt
    17101 ctgttgactc agcagctaca tccagggtcg tcatatgata gactgactgg aaatatggtc
    17161 caaatagcaa actgacagtt actaactgca aaagcttcaa ttttaaccct ttcacatcct
    17221 tatactcctt tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca atgcccatct
    17281 aattataatt taatttaatt ttaattttaa atcaaatgat atccttttta gttaaaaaac
    17341 accaattata caacatttat tattaaaatt aaattataaa attttataac gttctcattt
    17401 aacaaaactt aagttattaa gacaaatgaa taaacaacta cgctttcctc gaaacaaaaa
    17461 aataaagcac aaatgcgcat ttacgcactc atgctttatt cgtacaatta attcagataa
    17521 cggcagcgca taggatgcca ataacacttc ctaaaatagc tcctcctaaa atgtcagtag
    17581 gataatgtaa aagtaaaaaa atcctcgaat aagcgatcaa acaagcaaat aaaaaggcta
    17641 atattcctgc aactggactt aattcgaaca acgcagctac tgataaaaag gctagcgctg
    17701 tatgtcctga aggaaaagaa gaagtcttcg gtttacttgt gtaaaggaca agccgacgat
    17761 ttttttcata aggtcttttc cgtcttgtcg tttttttgat aaaaagcaaa ccaacaaaaa
    17821 aatagatcaa actgccgata agaatggcta gaccatattc tctagtatca ggtatcaaga
    17881 ctaaaacagc agcaaggagc aaccaaataa aaccaccatt agagatatta ctgataaatt
    17941 tcaaaaaatt gatttttcga ctatccgttt tccgttgctt ggcgaacttg ttgataatac
    18001 tatcatcgat tttagcaatc aaacgcaaca tgttattcct cctttttaag aaccttttct
    18061 tgcgtacgat ggataaaaag caggacttga aaataaaaca agaaactgaa acctaataaa
    18121 gcaaaataga ttggtcctgc aaaccagaat gcgacaagaa ggatcaacgt gcctgccaaa
    18181 tagaaaagcg cgcttttgaa atcattaaaa aacaagaaaa atgcagtaat ccaagaagct
    18241 tctttttgtt ccagttgaag caagattttg taactaaaga gaagcgcgct taatagaagc
    18301 aaaaacaacg tagtatacat caatgtatag ccaattcccc caccccaata gcggaaaatc
    18361 tgataatcga tcaatagaag aagaaaataa acacttgcta aactatcttg ctttccataa
    18421 agagagaagt tctcacgaaa ctttttccag aatagtgaac cgagcttttt ctcttcattt
    18481 tttttgtaat gataaatcat atacagaact gttcgacttg cagttcctaa tgtgaaaaca
    18541 ggcaagacgc acaggaacca tagtagctgt aaatggataa gacgtggaat ccattcaatg
    18601 atttctaaga aataatcatt ggctttttta ccaatcgatc gattcatcat ccttttgaac
    18661 ctcctgtcaa atccatccct tcaatgaaat aacgttgtgc aaaaaagaac agcaaaatcg
    18721 tcgggatcaa gacaagtgtt gctcctgcca tcagataatt ccattgtgtc gttgcttggt
    18781 tctgaaaaac ttgcaagcca atctgtaacg tgtatttttc ttgatcttgg atatataaca
    18841 gaggacctaa gaaatcattc caagctgcgt tgaaggaatt gatcgcaatg gtaatcaagg
    18901 caggtttcgt caatggaagc atcaaatgac tccaaatata caaatgattc gctccgtcga
    18961 tttcagcagc ttcgatcaat tcattattga tcgataagta aaattgacgc atcagaaata
    19021 tattgaacgc attgccgaag aacgatggaa cgatcagggg caaataagta cctacccagc
    19081 cgatcttgga gaataataca tattgaggga tcatcgttac aaaccccggg atcatcatcg
    19141 tagaaagaac caaagcaaac atcagctttt tcccaggaaa atcaagcttc gcaaatccat
    19201 aagcaataaa agaatttgaa aggacattac cgatcactac tagcacagtg atgaataatg
    19261 tatttcttgc atagcgccaa aaaggaaagg cggcaatcgt atccagatag ttttcccata
    19321 cgattttttc tgggaaaaag gtcggaggat aacgaacgat ttcttccatt ggtttcaatg
    19381 aagtaaacac catccaaagc aatggaatga tcaaaatgat tcctattact gtcatgagga
    19441 cataacttag tatttttttt gtttgccgat tcacactggt ctttttgaaa taatccatca
    19501 tttgccgtca cctccagaat aatagaccca cttaggtgca agcttcaaat tgacaaacgt
    19561 cagaaccaac acgatcagaa acaggatcat tgacatagcc atcgcataac ccatatcaaa
    19621 agctacgaac gccttgttcc acatatgcag attataaaaa agcaaagaat ttgcaggtcc
    19681 gcctgttcca tttttcgtca tgacataagc ttcttgaaaa atttggaaag aaccgatcgt
    19741 agaggtgatc acatcgtaaa aaataatagg agtgattaat ggcaaagtaa tacttttgaa
    19801 ctttccccaa acgcctgctc catcgatttc tgctgattca tataaagatc taggaacatt
    19861 ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa ctccatattt tcatcaaaat
    19921 caacgcaggt ttcgtccatt gtggatcaaa cagccaagct ggtccaggga tatggaacca
    19981 gcccagaaat gtgttgatca atcctgcaga tggagaaagc agctgcatcc ataagatgta
    20041 gactgctact cctgataaga ctgccggtaa atagaaagct gtacggaata cactgattcc
    20101 tttgattttt tgattgagta ctgtggctaa aaacacactg aaaatcgtcg tcagcggtac
    20161 attgaagatg acgaaataaa cggtgttgta taacgatttc caaaataaat catcgtgaac
    20221 gaacattctt ttgaaatttg ctagaccaat gaaattcatc ttggatgtca aattataatc
    20281 ggtgaaacta ccaataactg aaaaaagcat aggccctagc ataaatacca agaacccgat
    20341 aataaaaggc gagataaata aataggccca tcttgcttct tttttctttc ggatagataa
    20401 acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc aaaaatggat tattgatttt
    20461 gcttgactaa gttttctact gctttttgtg catctgccaa tccttctttt ggtgattttg
    20521 ttcctaaaag aatctggtca atctgttcat tcactaatgt cgtgtaatcc ggagcatcta
    20581 aaggaactgg tgtgatcacc gtatttttaa agttttcgtc cgccatttga tagatcattt
    20641 ttcctttatc cgttaggttg tcattattca ctaaattctc attggctgtc ttgctcgcca
    20701 tgatatcaaa acttttttcg ccgaattttt cttgtacttc tggagaagaa agatatttga
    20761 tgaattcata agaggcctca ggatcttttg ctccgtatgg tacttccaag acgaatccgc
    20821 caccccacga ccaatggcct gaaccttctt ctttttctgg gagctgcatc acgccaaaat
    20881 caaaatcatc cggtgcattt tccatcaaac tagagtaata attgatattt tgggcacgca
    20941 tggctactaa tccggaaata aatggatcag atacaccaga cccaaattcg gcttctaatt
    21001 tattgatcgt atttttccca taataatctt gccagtcttg gatccactcc aaggcttcca
    21061 ctttattttt agtatcaatc ttgacttttt catctttgtc aaaccaacta gtcccgttat
    21121 ctgcgtttag cgcccataca tcagccccta aattccataa tggataaaaa ccgatgcgtg
    21181 taaattcttt tccttctttt ttatctagtt tacgtgctgt ttcttctaat tcatcccaag
    21241 ttgtcggtag atcttgttca ctgattccag cttctttaaa taacttttta ttataaaaaa
    21301 tgacttgtgt atctgtattg aatgggatcg cataaggatc accttcatat tctactgtat
    21361 cccaaagctg cggataaaaa tccttagaag tgtctttttc tacatatttg gagagattag
    21421 ttgcttgctg tgcttctgct ctttgggcaa cggaatttat gtcctgtaca ataacatcgg
    21481 gtgcatttcc agctgttatt gcagataaag atttagtcca gatgtcaccc catggctgat
    21541 acttataatt tacatggatc tcgttttgtg attgattgaa atcctcaatg atttcttcga
    21601 tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa atcaatattt tttcttccat
    21661 cacttgtttt ttcagaagaa tttgctgcac ttgaactggc atcgcttgca ttacttgatc
    21721 cgcatcctcc taataaaaac aatgctgaac ttgccagcaa ccctgtaaac aataattttt
    21781 tcatatttta cctccaaatt ttaatgtaac cgatgccggt tttaatataa cgatatcatt
    21841 ttgttctgaa aataacaatt tataaggtat gattctaaaa aaattattaa tgataccaat
    21901 actttatata attcgcgagc gctttctttg aattttttaa tctttcgtag cagaatgaga
    21961 atgtattaaa aatagattag gaggaatttt gatgtttaga caccagaaag aattgcagtt
    22021 tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa tatctgcagg agctgatcgg
    22081 cggccaatat ggcgagatga gtgtagctat gcagtacctg ttccaaggct ggagctgccg
    22141 aggagaagaa aaatataaag atatgctgat ggacattgca acagaagaaa tcggccacgt
    22201 ggaaatgttg gcgacaatgg ttgctcgttt attagagggt gcgcctgttg aagatcaaga
    22261 agcagcgatg caaaaagatc caacaatcgg tgccattatc tctggaatga atccacagca
    22321 cgcgatcgta gctggcttag gacctcgtcc tgctgacagc gtgggcaatc catggagcgg
    22381 tgcctatatc attgcgagtg gcaacttgtt agcagacttt agagctaatt taaatgccga
    22441 atcacaagga agacttcaag tagcacgtat ttacgaaatg atcgatgatc gtggcgtaaa
    22501 agatatgctg tctgttctcc ttgcaagaga tagctatcat caaaatctat gggaatgtgc
    22561 agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc ccaagtactt tcccgcgtga
    22621 caaagaacga aaagacattg cttatgactt ctataacttc tcagagggag accaaagtag
    22681 tgaaggttct tgggctagtg gcaaagcact ggatggcgaa ggcgaatatc aatggttgaa
    22741 agaacctaag atcgaaggcg ctgcaccaaa attagaacca gtaacaccaa aaatgtatgg
    22801 gacacctcca ttcaagaaat aaatatctac ccgtagaaac acccgttttt tgttccacac
    22861 gctttgtgag caaaactgac taaagtcatt aatcataacc acccttcaaa agggtggttt
    22921 tctctagagc tataagctca aatcaccggc cagcgcctaa agacgctggc tttcatattt
    22981 tatgttcaag ccatattgcc tttgccagtt ttgcccacct cttaaaaggt ttatgtacta
    23041 ctccttagcc cttaaaagga tcgtcttact ctcgtacact cagcttatct aacgcactat
    23101 catgtttttc ttgttcttga atgtactttt tgatggtcgc ttcattttgt cctacttttg
    23161 tcacatagta tccttccacc caaaagtgtc gatacccaaa tttatatttt agatttgcac
    23221 aatctcttga tgatctcctg aatattggct cgatattgat tataaattat ttttcttcta
    23281 tactttggtg tgaatacaat gtggtattta cacatctact ttgtacgggc taaactattt
    23341 ccctttattg ctccacccgc atagcaagtg gttttgtatt tcaatcgctc ggcgcgctca
    23401 attaggtcta aagacaataa atctaaaaac ccgctgcttc actgacagaa acaacgggtt
    23461 ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat ctattttatt tttttgattt
    23521 tttataaatg gaaaatgctt caagaagtcc aagaaataaa gccgtataaa aacaagaacc
    23581 tatgaaatct ttatttatta gatattcaac agaaatcccg ataacactag ccaacaatat
    23641 atttatcaga agtgtaaaat aaggatgctt tttatagaat agaactagtg ttgttttcat
    23701 agactctcct ttcttcatat ttccaattaa tcacaaattc acggcattac tagaaaaatt
    23761 aaaggggcgc agaagtggaa aaatactctt cgactttccc tacaaaccag gccatcttgt
    23821 gtttaataac tataccaaat ctaaggtacc tctaaatcta aattccattt gtggcggtga
    23881 cctccaattt ttaactcttc tatctcccat ccagtataca taaccttgat acttcgacca
    23941 cttatatatc gaacacacaa atacctatcc attgcaggag tactacctaa cttagttgct
    24001 acttgcatct aaataacagc tgaaaagata ggacttactc ttagtaaagt atctgaacta
    24061 ttaattttta aactatcatt tttatcctca actacatgca catctgttgc aacttcactt
    24121 tctgtctcat ccagataaat gattaaggaa tcgggaattg caatatcttc agctgacgct
    24181 ggaatagttg agctaaatta gtcatcatat ctttattata aaataaccaa cagcaaaaaa
    24241 tacaatgaaa aacaaacttg gaactaatgt atcattaata aatgttgaaa aattcagcaa
    24301 ctcattactg tatgatttta gcagcccaac caacatatac cctataaaag ctaataagat
    24361 ggttaggaat cctttagaat ttttactcat tcatagacac ttccttaaat aacttttatt
    24421 atagaaggta ttgtaaataa taatattgac aaaaagtgac tcaaaataaa aaaaacctta
    24481 cattatactg gaatgaactg tattgatagt gtacctaaaa gctctactga cataagatcg
    24541 tgtaatcggt aaagtaattt tattaattat acgggatgta atcaattaga gaataaacta
    24601 atcataaaag gtacttttac attcataatt gtcgctttga gattctactt tggttcacta
    24661 tctgacccaa ttgcacattt tcgatcatta caagtattgg tgtcgttaga acaactaaat
    24721 aaaaactagc ggtagatttt tcagaatcta tgctaatttc tgtatggatg ttccagtgat
    24781 ggcaaatttc tcttttcatt gtttttcttg tcgcatgata tgcctttttg tgttattcag
    24841 ttaaactata gaaattcttt cctagacaga tcttgatccc aaattttacg tttttgagca
    24901 tcgatatagt ttaaaatgac attactcata tctaaagagt ttagattttt taggactaaa
    24961 actgtcagtt gttctttttc ttctttctgt taaagtattg tcgcgacttt tcgtatacgt
    25021 taagatatgt cgtgaaaaaa caagttttgt gacacaaaaa aacgaatgac ttgaggaaat
    25081 cacctcaatc attcgtttaa aatgctatat attctttagt ttaagatacg gaaattaatg
    25141 ccatcgacca cactagtaga atgaacgaca tttacttggc ctttttttaa tttcgttatg
    25201 tttagcatat aaaaatatta tagagtttac ttacaatgct taaaacatat actgtataat
    25261 ctgatcaatt ttagccatgt cttgcaatgg taagttttct atttttttga gttttcgttc
    25321 cttaaaatca agagatttta attgtgtaat aattacttgt ccttttatat ctagctcttc
    25381 aggtaatgag taacgtgtcg gtaaattctt tatagtggaa gtaattggac atatgaccga
    25441 aaacattgtt ttacggttaa attcataacg agagacgact agtcctggtc gtcttttttg
    25501 aatttctttt cctacggagg gatcaaaatc aatccataca atatcacctt ttttaggaat
    25561 ataaattctt tcttctgaca ctataaaatt tctctccctt caggagttaa atcttcccat
    25621 tcgtctttct cataatactc ggcctcttcc ccaccactaa atggatcttc aatttttgga
    25681 actaacgtaa ttgttccatc atcagaatac acaacgatat attcttggtt ttctgaaggt
    25741 ttctttccgt tatcagaagg taaagttacc actacagaac ttccttgtaa tctagatttt
    25801 gtaacaagca ttatcatcac tcccttaata aaagtatact atataacact aagtaacgca
    25861 aaattcccct ttgtaaacta tctagaaaat tgttttgttt aaagtatatc ttattggata
    25921 taaatagaaa taaattgtag ccagttcatt ttttctttaa ctaaaaaaat aatccaagaa
    25981 acattgaacc tatggatcca aaagctaccc aaatactata gctaaccccc cgctaccttc
    26041 aattttgcag ataaaacgct ttgggagaaa atttcaaaga caacaggcca tcctatcatg
    26101 cccgattata aacaaaaagc tggctattcc ttttgctctg acaaatttca attgggaaag
    26161 ttcacgaaac tagcaggtga agaagtttca actgttcgga tcaaagaatg cccgcttcaa
    26221 atagaaacat tggttcaacg aatttcaact agacaagact ttgctattgt agaacgtgag
    26281 atcgtagcta tttttgtaga agaagggatt ttacatgatc caactcatat caatgtggat
    26341 aaatggcaac ctcttatcta taaattcaga gaatatacaa ctgccaacca gtcgttaact
    26401 ttgaatttta gattccaaga attcaaagag tcataatgat aaacatcgtt aggtataaat
    26461 aaaaaagact cgcagcacga gtaaaaaaca aatagaagct atacattaga aaggtcgact
    26521 gtgaccctat agatgcagta tatgcagctt atgagcattt cttctgttct ctttgttttt
    26581 gggatctgtg ctgaagtctt tttcgttttt tgccgctctt tctttgaaaa atcgattatg
    26641 ttataccttc tccattacta attcaggcat ctgctgagta atgatttttc gcgcaaaaga
    26701 aatatctgtt acccaaggtt cgatttgttc ttcagtcaag acaagcggca ttcggtcatg
    26761 gattggtgcg actgtgcggt taggttcagt agtcattatg attgattctg ccattttttc
    26821 atcctgatgg ctacgataaa aaccgccaat atacactatt ttttccttat ttgaaaacat
    26881 aaatttgtct ttttcttcat tccactcatt gggaaaacac aacgattttg caaaaatggt
    26941 tgatgaaagg tctttttttc ttctactgtc tctgcacggg cattaatcat caattgtcct
    27001 tttttaaatc cggtaaatcc ccacttcatg atttccggag tgaggatgtt ttcttcctct
    27061 tttcttaacg tcacaacaag atttgacgga aaaacttctc cagctgctaa ttctgctgcc
    27121 tgcttcggtt gtttcgtctg ggcttctcga taataattct gaaggagctc tgactctaga
    27181 tcaaaaaaat aacgaccaca catcctaatc cctcccaatc tttttttcta tcatacactg
    27241 caacaagaag ttcgcaccaa caaaacgctt tcaatttttg tagaactaaa aaagttgtga
    27301 caagtgacaa actctccagt gaatggtatt ctcctgccaa ctcctcggtc acaagtcaca
    27361 atattcaaca aacatgagaa gctgcttttt gaaattgttc cttgtgactc ggagtgaaac
    27421 ggcaggctta cgacctctgg gtaaacggtg ttcaaaagcc agtttctcgg tattaagcca
    27481 aatttatgca aaaatgtaga aagctatttt cacaaaattt ttcttaatac tcgaaactaa
    27541 gcgacttttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    27601 gcccaaccaa acgaaaaata tgagcagcta tttttatttg gctgttctta ttacttgatg
    27661 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctaa ccttcggtat
    27721 tagctcaaaa aacggcaaaa tatgaggaac tattttgccg tttgtttctc taatactcag
    27781 gtcaaagcgc tgatttcaca acctctaata cacggtgttc cagaagtagc ttcttcgaaa
    27841 aataagccga aatttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct
    27901 tgaagctgac cacttctgtc acaacctcta attattaaag aaccttttgc gaagttaaca
    27961 atctttcttt gatcaattga aaattctgcc taagtagtga tagatcctgt gtgactttca
    28021 acaataagat acttccctgg atttcagcaa tcaacgcttt ggcttgacgg tctgcttctt
    28081 catcggacag tttttcttca gaaagtgcca ggatattttg tttcgtatac ttgacccatt
    28141 gattgactaa ttctagtagc aacagacggt attcttcatc cactgtactc aactcaataa
    28201 tcaaattacc tacaggacag ccatgataag tgatctgtgt ctcatgatca gcgaaaatcc
    28261 agtccaacat ttcttctaat gcttcttctg gctgttttcc agcatttaag atgtcttcga
    28321 acaattctgt ctgccatacg tccatatgcg catggatcac cgctagacaa acttcttttt
    28381 ttgaagaaaa gtaataataa aattgacctt ttcctgtatc agaagctgct agaatatctc
    28441 ggattgacgt ttcatgatag ccttttttat aaataagatc tttggtcgct tccaagatct
    28501 tttcctttgt tttcaatgat attctccttt ttcatttctc atagtcctat attatacagt
    28561 aaattcatca ttttttcttc ttaatcgttt aaaaaagact gtgccaaatt atctgacaca
    28621 gccctcacta taaaactgtt ctcatatagc tagatgttca ttgattcaag aactgcgagg
    28681 acaaaagcaa ttcctatctt atgatagttg ctctaatact tcctctgttg tcatcacttg
    28741 atctgcaata aagccaaagt tcgtcaacgc tgcatccatt gcttcttggc caggagcacc
    28801 aattgcatct gtcgctacca caacattgaa tccactctct accaattcac gcatatggga
    28861 atcgctacac aagtttccgt tcattccacc aagaattact gtgtcgattc cattttttct
    28921 caattggagc gccaaatcat tgctttctgg tccaaagatt ttatgaggag agctgatgat
    28981 cgtattttca tccaaataag gtttcaattc ttcgataaaa tcggctcctg aaccttcttc
    29041 cactgattca tattgacttt ttctagcaaa catctgctgc tcaagcatca tatgttctcc
    29101 tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa taatgaggag aaataaagag
    29161 tttgtatcct ttttcttttg cttgttttac catttcaatc atattttcga tcgtattgta
    29221 tttttctaag atatcttttg tcaaaccaaa gccaccgcct gctggtttca agaactcgac
    29281 ttgcgggtca gtgagaacta ctgctgtatt tttattattg aacatggtgg attctccatt
    29341 cgttatttat tttaatacat catttagagc ttcactcatt gtcggatgag aatagatttg
    29401 atctctcaat aacgtataag gaagttttcc attcatagct agcgaaatca agttgatgac
    29461 ttcatgagaa tcttccgcat agatacttgc ccctagaatc aagtcagttt cagggtcgac
    29521 taaaactttg aatacacctt taggatcttc taatacttgt gctttaggga caccgcttgt
    29581 catatacttg aataatttgt aattgacgcc ttgtgctttg gcttgttttt ctgttaaacc
    29641 tacattggat aaagtcggag tgataaatac actgtaaggc acagctttcc ggttattcgt
    29701 tgttcgttta ttctcgccct tcaactgatc cataatgatt cgataatcat ctagagagat
    29761 ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc caaatgttgt ctgcatctgt
    29821 acgaaggtat tcatccacag cgattgctcc gcgatctgtt agtttaacat ctgtattttc
    29881 taatccaagt gatgctgtac ttggttttct tccagttgca gccaaaactt ttgacgcttg
    29941 gattgttact tcttggccat cttctgtaaa ggtgacggca gctgttgatt cttggtctgc
    30001 tacttgatct acagatacac ctagatggaa ttcgattccc gcatttgtca tgtcttctaa
    30061 aatcatttgc gcgatatctt catcttcacg tggaagaaat tcagaatggg catcaagaac
    30121 gacaacttta gatccgtatt cattgaacat tgatgcaaat tccaagccaa tatatccagc
    30181 cccgatgatc actaattttt ccggtaattg ttcctgatcc atcgcttgcg tagaatcaat
    30241 caagtagcga cttgtattca atcctttgat tggtaaaatg attggttcag aaccagtatt
    30301 gatgaaaatc cgttcgcctt caacaatcac tttttcacca ttattcgtca cttcgatttg
    30361 atggttagaa agaaaacgtg ctgtaccgtc catcactgtc ccagttgcct catcgctgat
    30421 catatgataa tttttggcgc gaagcatgcc cgtcaatttt tctttccgag caacagcctc
    30481 tgtaaagtcg attcctcttt ctccattgaa aatcaatgat ttagaaggaa tacagccgat
    30541 gttgatacat gttccgccat acatacgtgt tgacttttca acgactaaaa ccgattcacc
    30601 ttttgaagct aacgccttcg ctaaagtctt accgcccttt ccaaaaccga tcacaatatt
    30661 ttgatatttt tccatcattt ttccctcgct tcttttttac ctaccgattg gtacaaaaat
    30721 atgataaccg ataattgtct attctccaaa tcatctgctt tatttgtcaa aaaaaaccag
    30781 acaaaaagcg gtcctttcac gtttttagtc tggttcgatt tcctttattc ttctaaacaa
    30841 tcgttacttc tacatcgata tttcctctag ttgctttgga gtaagggcag aattcatgcg
    30901 catcttctgc aattttttgg gcttcttctt cgctgatgcc atcgatatgc acttctaata
    30961 ctacaccgat ctgatagccg ccatcatcta aattataaag agagacacgt gcttttactg
    31021 tggactctgc tgtcattcct gcttctttga tcatatgttg taccgcccca ttgaaacaag
    31081 aagcataacc tgctgcaaat aattgttctg gatttgttcc ttctgcatgg attcctggag
    31141 gagtgatttt catatgcatc gagccatttg gtgcctcgac ttctccattg cgcccgccat
    31201 ggttgatcat tgctgtttcg tatgcttttt tcattgtact gctcctttcg attgaccatt
    31261 tgctacttgg ctataattat agtgtatgac aagtaaggcg ctttctacaa aaattctgct
    31321 tattcgaata ccagaatgcc gtcttctact atttttccaa aaaacaaaaa aatactactc
    31381 gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa tcttttctac ataatctttt
    31441 atatgattga ttttaagata catatatacc agaaagttga ccccataaac caatactgtt
    31501 aaaaaaataa ccatattcgt taccattttt tcctcctttt gctggtctcc ctaccagcac
    31561 tcgatttaac cataccactt tttttgccgt ccaagcaact agcctttcaa aaagactggt
    31621 ttattctgct gttaatgatt caagatcaga aaaatgtccg aataattttg ctaattttac
    31681 taacgccaac ttgttctttc cgaattcatc attttctatc tgtaatacta gtaacggctc
    31741 atgtaagctt aaacggatca aaaaccagcc gtttccgtat ggctcttcta gatcatatcg
    31801 aataccttct tcattttccg ggtcgaagtt catccctttt tcttcttcca acagtcgctt
    31861 catttcttct aatatcttgt ttccttgttc gcgtggttca tcgctgaaca attttaatcg
    31921 gatttcttgt acttctacgg gctgtttcaa ctctttgatt tgttcatcta atgtgatacc
    31981 ttttcttttt aattctggca aaagcatcag gatctttgcg atgacatatg caccatcatc
    32041 cagaaaatag ttttccttga atgcagcgtg tccgcttgtt tcgatggcta gctgtgtatc
    32101 tttcccaaga cggttaagtt ctaatgcttt attgattaca tttcgatagc cggaaatata
    32161 acgaacttgg atgcctcctt tttcttcaat gaatctttgt agatgagaag aagtagggga
    32221 attcgtcacg attcttgttc ctggatgctc ttttaatacg attgtactta atactgcgat
    32281 caaattattt cgattgatca ctgttccaga agcactgaca actgcagaac gatccacatc
    32341 tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa acagcctgct tgatactttc
    32401 catcgcttct ttattatcag gatttgggat atgattagga aatcggccat ctggttccaa
    32461 aaattgggaa cctgttgaat ccgcacctaa gctattcaat actttttcag caaagaatcc
    32521 accagcacca tttccggcat caacaatgat tttccaacca tttagtggtt tgctttctag
    32581 tgttccaatc ccctttttaa tttttgtgac taaatcttgc gcataaggtg tcaaaaggtc
    32641 tgctttttct acgcttccag tttgttcgct ttttattttt tgctgctgtg ataggataaa
    32701 agcaatatct tctttttctg ctcctccttc tgaggtaaag aattttattc cattataata
    32761 atagggaaga tggctagcag tcagcataac cgcagcatcg cacgagaact gttcaaattg
    32821 agtggacata aacatagcag gcgtagtagc taattcgaaa tcgatgacat gtatcccttg
    32881 ttctttcaga ctttggatca acgcctcttt cagtgctggt ccgcttatcc tactgtcatg
    32941 accaatccca atcgtccatt ttttatttgg atcggttgat ttcttctttt tctttatcca
    33001 gtttgctagt ccccagccga tcttttttac catgtctgtc gtcaagttta cttcatatcc
    33061 ttcaactggt aatgcgattc ctcgaatatc cgatccattt tgcagcgctt tcatatcttt
    33121 catataaaag tcctcctcat ttattccctc attttttcca gcttttctgc aaatcgctgt
    33181 gcttcttctt ctgagcggca aataataatg aatgtatcta caccggccat cgttccgaca
    33241 acttcagggt aagccacttc atctagccaa ttcgttacca catcggcacc atttcgttca
    33301 gtcaatacga taaccatgaa ctgaacagac tggatacgca aaacctcgcg tctaattgct
    33361 gtacgcaaac gttcttcatt taaagaaaca gatggctggt taaataatgt atagcgtact
    33421 attttgtttt cattttgact ttttactaac ttcatttctc gaatgtctcg agaaatcgtt
    33481 gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa gcagctcttc ctgtgtccca
    33541 ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc gctgtttctt tttcatatta
    33601 gacacctctc aatgttgatt atagcaaaag ataagtaata tttcttcata ttttggtatt
    33661 ttttactttt attttttgtt ttcattcagg ttgctaacct ttttctattt taacacgcaa
    33721 aatcgtcttc attttttcag gtgctgattt tcttggatcg ctgatgtaaa tctctttgtg
    33781 ttctttagaa gctctttcat agccttcttt tgcgatgaat gcttctaatt tctcaaacgt
    33841 tgctggttct tcggcatagg gcccgacgtg taagatttgg gcacaaagac cttcttccat
    33901 ctttttgaat actacttgtt ctttcaagtc ttccggaagt ttgctagaac gctgaatggc
    33961 ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg atcatcaatt gataagaaaa
    34021 atgttctttt ttcatcactg gttcatgcaa atacttttct tgcagtcccc attgtccttc
    34081 cagtggatag acagtataca tctgataatc aggaatcagc caattttttt taggcgacat
    34141 gcgtattgca tagctgactg catatagaca ggctactctt cgtttaaagt cttcctgatt
    34201 cgggtcgccg atccctctga tgctaaagaa tgatttttta ggaacctcta gtatttgcgg
    34261 gctttttttc atactatagc tcttgtcttt acgccattca attttttcca tttttcttcc
    34321 tccgcgaaat tccatttagt tatttattat catacctctt tttgagtgac ttggctaatc
    34381 gtgttgacac tatttttcag tttgtgctag aataagtgaa gtaaataaat cgtacagata
    34441 acacgtttcg gcgtgtcgag agagaggcgg tttctatttt tataatagaa atcactctct
    34501 ttttttacga aattttttag aggtgatgta aatgacaaaa caaaaacagc tgcttttagt
    34561 acctgcagta attgcaacag ggatgatgtc ttttgcgggc gtattgatcg agacggccat
    34621 gaatgtaact ttcccaactc ttatcagaca gtttgattta acgactgcac aagtccaatg
    34681 ggtaacaacg atttatcttt tgatgatttc gatcattgtt ccgctgtcta cttatttgaa
    34741 tcgcaatttt agtctcaaaa aactcttttt agcttcaaat ctgcttttct tggcaggtat
    34801 aatgatcgat ttcttctctc ctaccttcat ggtcctattg tttggtcgat tgctccaagg
    34861 tgcagcgaca ggtatcgctc ttccgttgat gttccatatt atcttgacat ttgcccccct
    34921 caatcgacga ggcgcgatga tgggtgtcgg tactttgaca acggccattg ctccagcaat
    34981 tggccccact tatggaggca ttatgacttc acattttaca tggaatcata tcttcctgtt
    35041 tcttgtgccc gttttgtttc tttctttgat tatcggctgg tttgctattc ccgagatggc
    35101 agtacaaaaa agtggttatt tggattggat aagcgtttta ggaatttgtc ttttgtttag
    35161 tggaagtcta acatttttga gtacgttggg aacagtttcc agctggattg ctttaatcat
    35221 cggcggtatt ggctggttcc ttttttacta tcgaagtaaa aaagtggagc atccactgat
    35281 ccgactgtct attctttcaa ataaaacatt ccgcttgttt ttgattggct tcttagttta
    35341 tcagttttta ttacttggtg tatcttttgt cttgccgaat ttccttcaga tcgttcaagg
    35401 aagtagtcct tttgtcgctg gattagcgat gcttcccggc gcagcggttg gtgctttgct
    35461 ttctcctttt tccgggaaaa tgctggatca atatggtccg aagaagccaa tttttgctgg
    35521 gctgattctt tctttgattg gttggctagc attattccta ctcataggac acgcttcagt
    35581 gggtgtattg attgcttgtc atgtgtttta tatgatcggt atcggattat cttacagcaa
    35641 tatgatgacc accggaatga atgcactgaa tgaagaacta caaggagacg ggaacgcaat
    35701 gttcaatacc ttgcagcagt tttctggcgc tgtggctact tcattagtag cggtcattat
    35761 caattacgtc cagcatcata cttcccataa ctatgaagtg tctactacat taggatcaaa
    35821 agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc tttgctcgat tcgcctatta
    35881 tctgttcttt gctaaaaaag cgtagattcc atatacggca ggtgttgcat ctgccgtttt
    35941 tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta tggaaaaaat tgccattatc
    36001 ggtggcggga tcattggtat gaccctagcc agttacttag atacatcaaa attcaatgtt
    36061 gctttatttg atgaagggac aggacaggca actaaagcta gcgctgggat tatctcccct
    36121 tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg ctaaagacgg cgctgctttt
    36181 ttccctaaac ttataaaaga tttccaactt gatcaaacga tttacgcaca atcagggaca
    36241 cttttgcttc gacctgaaaa agatttagct gaattggctg ttcttgccga gaaacgaaaa
    36301 gaagaagcgc cagaaattgg aaacatcgaa tggctttcac ctgaacagac atctcaagca
    36361 cttccgttat tgaaaccagc agtttcactg cgtatcagtg gtggtggtcg tctggatgga
    36421 aaagcatatt tagaacatct tggtcagcgt ttagcacaaa aacaaatgga gcagatccag
    36481 gcaaaagtca ctttgcataa gaagagcgat tcttggctca tcaagggacc gtttggagaa
    36541 tacacagctg atcgtgtaat tttgacacct ggcccggcac ttaagccttt attacaagaa
    36601 ttaggctatg ctgcagatat acgtccgcaa aaagggcagc tcttagcatt tcagacatct
    36661 tataagatga gtcaagactg gcctgttgct atgcttgacg gagaagctga tttcattcct
    36721 ttcatggatg gaaagatcct tttaggtgct acccatgaaa acgatcaagg ctgggatttg
    36781 gctcctacta gcgaagctta taagggattg actaagagta cagaacagtt cttgactgat
    36841 ccagatcaga taaaaaactg gccatacgag ttccgtgtag gtacccgtgc ttatacgtca
    36901 gatttttccc catttttcgg cgtactgcca gatgatccaa gcttactggc tgctagcggt
    36961 ctaggctctt ctggcttgac cacaggtcct tatatcggct atctattagc caacttctta
    37021 aatactggtc attggcctac ggaagattat caaaaggaca tctccaccta tattacgaaa
    37081 aatccatcat agaaaaacag ctttttcaag taaggatccc gagcgatatt gcgcatggat
    37141 tacttcttga aaaagctgtt attttagttc aatttctgaa tcaaattctc caaactttct
    37201 tttgcttcat ctggcaagag atcttttcct atctgagctt caatgaatcg taaacgaata
    37261 tcaagtcgtt gtttcaccag atttttatag gacgtatcct caaaagaaac aggaaaatct
    37321 ggtataggta aatagtggat gtcttcaaat ggaccaaacg acttgaattc tgccgtttgt
    37381 tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt tgattttttt atcatcgaaa
    37441 tatccagtat tcaggacata gcaggctgtt tgctgtttca aaaacaactc tcgaaaagct
    37501 tgatagtctt ccctaagagg atagacacgg aaaggattag caaatggttc aatgactagc
    37561 tcatctagct gtctttttgt agtgatattt tccgcgtctg atcgttttgt tgctaatgtt
    37621 gcaccaaaag acgctgctaa aactgggtca ttgatcttga taattggcgg aagcgaatca
    37681 tctttcatga tccaaaaaac agcatcgatc ttttcgctaa gatgatcgac tcggtcgggt
    37741 gtcgcataac gtgatttgac tgtccgccca tttccgtttc gtatatctcc tgttactagg
    37801 actttttctc ctttacttgt acatgtcacg cccacattct gacaagtaag gaaagaagaa
    37861 atggccggat catcagacgg atagtcttgt gttttatcaa agtaagaagg ttccaaggca
    37921 gtagcaaaac cattttttcg agaaataacg aaagcatcgt catgcaagat cgaaacagag
    37981 tgaccttccg tatgttttgc taaggtaatc gttgattttc cagaaccgga caacccaaat
    38041 acagccatcg tataggtctg gtcattacta gcatattgtt tcatcccacc gtggcaggca
    38101 ataaagccat tgcgatttcc caatgcccaa gcaagcgtca aagtcgcttt ttttaattct
    38161 ccaaagtaac gtaatcctag cacagccgct gtattatgga gagcatcaaa caaaatgagt
    38221 ccatttggaa atgtttcatg tgtccattcg ggatcacaaa agatataaag atctccctca
    38281 tcaaaggaaa cagacttttg ataagtctct aagatcttcg aattcgctgg ctgaaaattc
    38341 agcaaataag aatacaaatt gttctcaaat ccttctggaa tcagcaaatg actttgcaca
    38401 gaaaattctt cagataggcc aacgattact tttgtatggt aaaactggcg cttgcgactt
    38461 tgatacacag cttctcttaa aattttctcg tatttgcttt gttccttgtc taagtcctca
    38521 aagatccgcc gagcacttgc tgttcttccc acaatgcttc catcattgaa tactagtgtc
    38581 ctaacatcag ctggtaaatc tagttcttca gcatgtgaga ttgttaaatc tgtgatgatc
    38641 gttcctggac tcccttttgc tagttgataa gctgtcttcc tatcaaaaac ttcatgaaca
    38701 ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac tggataacag tggatttcca
    38761 cttgtgattt cgtttaattc gagactctct tttgtactca tggctcctcc tcattcccta
    38821 aacaaaaaaa cgttttcttt attatacatt attcgagttt ttatttgtac tgctgcgctg
    38881 tgggaagcaa acgagttatt tttcacacaa aaaaaccgag aacttgaata aacaagtttc
    38941 tcggtttcat cattttcctt ttatcggctt agtaccagcc gtttgctaac cagaatgttt
    39001 tagcagcatc ccatgaacca taacgagatg ttacatattg ttctgctaca cgttcttgat
    39061 tggcagctga atagtcgccg tttaagtatg aagcatctaa ttgataacgg ccgatgtaac
    39121 gtccgttagt agctgtgtat gaaccgcctg attctttttg tgcgatccat tcttttgcag
    39181 agcttgaagt tggcgctgtt gcagcagctg gggcagcagg agctacttca actgtttcaa
    39241 caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt tgtagatgct gtattttctg
    39301 ttgttttgtt ttctgttgtt gctgaagaat catttgaagt tgggattgtt aattgttcac
    39361 caacataaat gcggttgatg tttgcgatcg aatttttttc agcaattgca tcgatcattg
    39421 agttgtctcc tgcaaatttt tgtgagatct tagataatga atcacctgat tttactgtat
    39481 aaacttcatc agcgtgagca tttgtagttc ctacaaacat tgcagtagca ccagcagcaa
    39541 aagttgtacc taaaacaatt ttcttaaatg aattcatatt aaactccttt ttttctccgt
    39601 tttttattct ttcgacgcta ttaactttat catttagaaa tatttcatag gtaaaagaaa
    39661 catgacattt aatgacaaaa taacgaatta tttaatattt gtaacattta aacaaatatt
    39721 tttttgatac aaaaccataa aagccttgtt gtataagcaa agtaatcgtt tttctatgtt
    39781 ttt
//
LOCUS       DAZUMT010000014        37244 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000014 DAZUMT010000000
VERSION     DAZUMT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.014"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..839
                     /locus_tag="ACG8FM_000663"
     CDS             <1..839
                     /locus_tag="ACG8FM_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5093350.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1192..2040)
                     /locus_tag="ACG8FM_000664"
                     /pseudo
     CDS             complement(1192..2040)
                     /locus_tag="ACG8FM_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2193..3305
                     /locus_tag="ACG8FM_000665"
     CDS             2193..3305
                     /locus_tag="ACG8FM_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5093351.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="ACG8FM_000666"
     CDS             complement(3383..3676)
                     /locus_tag="ACG8FM_000666"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS5093352.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="ACG8FM_000667"
     CDS             3844..4158
                     /locus_tag="ACG8FM_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS5093353.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="ACG8FM_000668"
     CDS             4179..6158
                     /locus_tag="ACG8FM_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5093354.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="ACG8FM_000669"
     CDS             6171..6497
                     /locus_tag="ACG8FM_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS5093355.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="ACG8FM_000670"
     CDS             6513..7031
                     /locus_tag="ACG8FM_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093356.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="ACG8FM_000671"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="ACG8FM_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5093357.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="ACG8FM_000672"
     CDS             complement(8467..9972)
                     /locus_tag="ACG8FM_000672"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS5093358.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="ACG8FM_000673"
     CDS             complement(10064..10654)
                     /locus_tag="ACG8FM_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5093359.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="ACG8FM_000674"
     CDS             10833..12479
                     /locus_tag="ACG8FM_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS5093360.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="ACG8FM_000675"
     CDS             12528..13247
                     /locus_tag="ACG8FM_000675"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5093361.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="ACG8FM_000676"
     CDS             13726..14673
                     /locus_tag="ACG8FM_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093362.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="ACG8FM_000677"
     CDS             14685..15605
                     /locus_tag="ACG8FM_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5093363.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="ACG8FM_000678"
     CDS             15646..17103
                     /locus_tag="ACG8FM_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5093364.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="ACG8FM_000679"
     CDS             17448..18062
                     /locus_tag="ACG8FM_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5093365.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="ACG8FM_000680"
     CDS             18055..19779
                     /locus_tag="ACG8FM_000680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5093366.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="ACG8FM_000681"
     CDS             19776..21227
                     /locus_tag="ACG8FM_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5093367.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21699..23852)
                     /locus_tag="ACG8FM_000682"
     CDS             complement(21699..23852)
                     /locus_tag="ACG8FM_000682"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS5093368.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23854..24900)
                     /locus_tag="ACG8FM_000683"
     CDS             complement(23854..24900)
                     /locus_tag="ACG8FM_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5093369.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25050..26339
                     /locus_tag="ACG8FM_000684"
     CDS             25050..26339
                     /locus_tag="ACG8FM_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS5093370.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26352..29054
                     /locus_tag="ACG8FM_000685"
     CDS             26352..29054
                     /locus_tag="ACG8FM_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5093371.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29039..29896
                     /locus_tag="ACG8FM_000686"
     CDS             29039..29896
                     /locus_tag="ACG8FM_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5093372.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29967..30824)
                     /locus_tag="ACG8FM_000687"
     CDS             complement(29967..30824)
                     /locus_tag="ACG8FM_000687"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5093373.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31035..33206
                     /locus_tag="ACG8FM_000688"
     CDS             31035..33206
                     /locus_tag="ACG8FM_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5093374.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33274..33615
                     /locus_tag="ACG8FM_000689"
     CDS             33274..33615
                     /locus_tag="ACG8FM_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5093375.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33891..35645)
                     /locus_tag="ACG8FM_000690"
     CDS             complement(33891..35645)
                     /locus_tag="ACG8FM_000690"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093376.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35656..36492)
                     /locus_tag="ACG8FM_000691"
     CDS             complement(35656..36492)
                     /locus_tag="ACG8FM_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5093377.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36494..37177)
                     /locus_tag="ACG8FM_000692"
     CDS             complement(36494..37177)
                     /locus_tag="ACG8FM_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS5093378.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12013 a         6553 c         7638 g        11040 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tctacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctgaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaaa aaaagccaat
    21241 gtaatgaaat aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact
    21301 tgaggcaagg cagctttttc atgacctctc gataaacgga gttcaaaagc cagtttctcg
    21361 gtattaagcc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttaata
    21421 ctcgaaacta aacagctttt tcacgacctc tccactaaat ggtgttcaat cagctgaccc
    21481 tcggtattag ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa
    21541 tactcaggtc agaacgctga tttcacaacc tctcaataaa cggtgttcaa aagcttgcat
    21601 ctgcggtaat aagaccaacc aaacgaaaaa tatgaggatc tatttttgct tggctgttct
    21661 tattacttga tgcaggacag ctttttcaca acctcttgtt aattatagtt tgataaggaa
    21721 aaaggcaaat cttttgcttg atatttctta gggtgaggga tgatccctaa ttggtaagag
    21781 atcgtgccgc ctttcagcaa atcttgatgg gtaaaatata atttcgtata ttctctgtcc
    21841 atccgtttga ttttatggat aaattgttgc tgcggccgat taggcgttgc ttcaatcgtc
    21901 aattcctttc catttgacaa aagcaaaaca gccttgtcaa ttaatggcat accgataaca
    21961 tattcaccgg ttccaggcgt gacaggataa aatcccagac tattgaagat ataccaagcg
    22021 gccatacttc cattgtcctc atctccaggg taacccattg gagaatcgtc gaaggtctga
    22081 gtcatcaact gtttaagcag tggctgtgcc atttcaggtt gtcctatata actgaacaag
    22141 taaggatagt gaaaacttgg ctggtttgaa atagctaatt gtccaaattc tatcgcggcc
    22201 atctcactca tttcgtggat ctcaaaacca tatcctcctg ggtcaaacgt tgggggctga
    22261 ttgcacagct caatgagttt ttctttatat ctctcactgc caccatattt tccgatcagt
    22321 cctgaaaaat cgtgataaac agcaaagctg ctttgccaag cacttccttc tgcataatct
    22381 tgtccccaac gattagctga aaatggttct ctgaacttgc catctttatc ttttgaacgc
    22441 ataaatcctg actcatagtc aaagatattt tgatagttca atgattgctg ctgatatttc
    22501 ttcgtcattt cctcttcttc tagtaccttg gcaacttggc taatacaaaa atcgctataa
    22561 gcatagtcta atgtgtgatt gactgattcg tggtagtgat taggaacata gccatatgtc
    22621 aagtaatctt ctgttccttg gcgtccataa tttggatgtt tgctttgaac cgtagcacct
    22681 ttttccattg ccgttagaaa ttcaggcatc aagtctcgtc ggatatcttt tacagcagca
    22741 tctgcaatga ccgcatcgat caatgtccca ggcataagtc cacgttcatc aggagacagc
    22801 cattttggca aatagccact ttctctatag ctgttcaaaa aaccttccag catttcttcg
    22861 tatttttcta cagcaatcaa agaataaaga ggataaaccg ttttatacgt atcccaaaac
    22921 ccattatttg tatagagaac accaggtttt atagatcgac tagtcgtatc atagtggatt
    22981 ttttgcccac taggatcaat ttcataaaat gtttgtggaa ataaaaacac ccgatataaa
    23041 ttatgataaa acgtccgcaa ttgttgttga tcatggtgtg tgagctgtat acgatcaaaa
    23101 taggactgcc attgttcttg actgtgagca aggtaagtct ctttgctttg gttttgttct
    23161 agttccaggt tataagctgc ttgttccgag ctgataaaag atgtcccaaa tcggatagtt
    23221 tgtttttttg tctgttcaaa atacaacggg atcgagtcgc tatttttgtt ggaaacagct
    23281 aactcatcaa ttgttaaagg atattcaaat ctcaatataa aataaaaaga aaaatcagga
    23341 tcttctgaac cagcaaaatg tgtgattttt ccttccactg tgtaaggatc tataacaaag
    23401 agctgatggc gtcccggaaa ggataaaagt aaaccgctgt catttttgct gtaatcaata
    23461 gataagattc cgccatacat gctaggtatc aatgtagaac agatgccata acgtaattga
    23521 cagatcgaca gatgagtcgg gcaaaatata ctttcttctg ggcggtaaga actttgagca
    23581 tggaaaaggg tgttctcctg tagttttcca ttgatcggcg taaacagcat atgactgaaa
    23641 tcacccatcc aaggacttgg ttgatgggta agacggtagc cttgaaaaac gcggtcatca
    23701 ggatgaaacc accagctgcc gttttgatcc gtagtttgcg gtgcaaaata attcatccca
    23761 aaaggaacgc cagtataagg ctggcagttc ccattagaga aatttggctg attcgctgtt
    23821 gcatgtctag tatctatttg accgatattc attttatttc tcctttgttg attgtctttt
    23881 gatgaatgaa actggaaatt tactatcgga tggtcgatct tttgtctcta accaagtgac
    23941 caaagcatct cccgcaagct tacctaactg ttcaaaatct tgtgcgatgg ttgtcagtgg
    24001 cgggtcaaca agagctgctg cttggatatc atcaaatccg ataattgaaa aatcttcagg
    24061 gatatgataa ccatgctgtc tcaagagatt catggtctgg atagcgacaa catcgttttc
    24121 acaaacaaaa gcagttactt catacttagt gatataagaa agcaattcat ctaatgtcgc
    24181 gcttttgtca tccagtgaag tgtggaaagt caaaccagct ttgtccaatg cttcaaggta
    24241 accaaggtaa cgctgtcgaa cggattgtgg atgagtctgc gtaccaaaca aataagcgat
    24301 ttttgaatgt ccctgttcga tcaaataaga ggcagcaagc gcaccgcctt ggaaattatc
    24361 ggaaagaacg gtaggaaaag gtaactcata gattttttta tctaatatga cgacagggaa
    24421 ttctttcaat gaaagctcaa aaagtaagtc aagatgttgt tctgtatcaa gagcataata
    24481 aatcaatccg tcaaattcat tgattagctc ttttgctgtt ttattttgta gaaagtcaag
    24541 cgtcgtcatc atgacatcaa tctgtttatc ttgcatcaca gggttcaatc ccttgctaaa
    24601 atcaccaaca gacaaatcag cgacatatgg aagaagaaac agaatccgtc gtgtttttgt
    24661 aggagaagca agaatctgtt ccggagaagc ggatttaacg aaactgcctt tcccgcgaac
    24721 gcgataaata agtcctaatt gctctaattc agtcaatgca cgtttggaag tgattctgct
    24781 tactttatag gtacgtgaaa gttctttttc agttggtagt tggctgtcta caggtaattc
    24841 accagttaaa atagccgttt gcaaatcatt aaatattgtc tggtacaatg gttgagtcat
    24901 ataaacacct cttaatgata tatcatattc aaataatacc ataatggatg aaaaagtaaa
    24961 taaaaagata ttttttgaaa gcgtagacat aattggctgt atgaagtata atctttaaag
    25021 atatatcaaa tagaaagaag gttttattag tggtttataa agatatacca aaatcggtgc
    25081 agaaatttat ggaagatatc acacaaaaat gtggggatat ccatgcggac tgggcaaaga
    25141 attttacagc agcatttgcc aatactctgt tgacaacagt caaaaagcaa aatgacggaa
    25201 ccaccttttt attgacggga gacattccag cgatgtggtt aagagactct actgcacaag
    25261 ttcgtcctta tttagtgatt gcaaaagaag atgaggaatt agctgacatg atcagcgggt
    25321 tagtcaaaaa gcaatttttt tatatcaata tcgatccata tgcgaatgcc ttcaacgaag
    25381 aagctaacgg tgctggccac caaacagatc atacaaaaat gaacgattgg atctgggaaa
    25441 gaaaatatga aattgattct ttatgctacc caatacagtt ggcctatctt ctttacaaaa
    25501 atacaggaaa aactgatcaa ttcaatcaat cttttattga aggtatcaaa aaaatcttgg
    25561 acgtgttcaa aacagagcaa gatcatgaac actctcctta tacatttgta cgagacacga
    25621 caagaaaaga agatacgctg atcaatgacg ggcgaggtgc aaaaacagct tttacaggaa
    25681 tgacatggtc tggtttccgt cctagcgatg atgcttgccg ttatggatat ctggtaccat
    25741 ccaatatgtt tgcggtggtc gtgttaggtt atgtggaaga aatttttcaa acgatactcg
    25801 atgataaaga aattgtacag caaacacaga aattgaagaa agagatccaa gaggggattc
    25861 agaatcacgg acgaacggtt aatcaaaaag ggcaaacgat ttatgcatat gaagtagatg
    25921 gaatgggaaa cgccacagtc atggatgaca gtaatgtgcc caatctgatc gccgcacctt
    25981 acttgggata ttgttctgaa caagaggaag attatctgca aacaagaaga acattgttga
    26041 gtaaagaaaa tccatatttc tatgaaggaa agtacgcaaa aggaattgga tcctcccata
    26101 cgccagaaaa atacgtttgg ccgattgcat tagctatgga aggaatgaca acgtcagaca
    26161 aatctgaaaa agagcggata ttgaatttac ttgtagcaac tgacgcagga acacacctta
    26221 tgcacgaagg attcgatgtg aacaatcctg aaaattatac gagagaatgg ttttcttggg
    26281 caaatatgat gttttgcgaa ttagtcatgg attactttga tattagagta gaaaagtagg
    26341 aggtatcgta aatgaaaaag aaagtatata tcatttctca tagccactgg gatcgtgaat
    26401 ggtacatggc ttacgaacag catcacatgc gactgattga attgatagac gatctactag
    26461 agctatttga agttgatccg tccttcaaca gttttcattt ggatgggcaa acgattattt
    26521 tagatgacta tcttcaagta aggccagaaa aaagacaggc aatccaacaa gcaatcaatg
    26581 aaggaaaatt gagaattgga ccattttata tcttgcaaga tgattttctt atcagctcgg
    26641 aatcgaacgt gagaaatatg ctgattggca tggaagaaag ccgtaaatgg ggaacccctg
    26701 tcatgttagg ctattttccc gatacatttg gaaatatggg acagacgcct caattaatga
    26761 aacaagctgg tatttcagca gcagcgtttg gccgtggtgt aaaaccaatt ggctttgata
    26821 atcaagtttt ggaagcagaa aactattctt cccaatactc agagatgtgg tggaaaggcc
    26881 ctgatcaaac agcaatcttt ggtctgctat ttgccaattg gtatagcaac gggaatgaga
    26941 ttccagtaga aaaagaagca gctcttgcct tttggaagca aaaattagcg gatgcagaac
    27001 aatatgcctc tacgaaccac ttattgatga tgaatggtgt tgatcatcaa ccggtacaaa
    27061 aagatatatc aaaagcaatc catctcgcaa atgaactgtt tccagattac gagtttattc
    27121 attctaattt tactgactat ctggaagcag ttcaaaaaga tgtgccagaa gacttaggct
    27181 cagtagaagg agaattgaca agtcaagaaa cggatggctg gtatacttta gccaatacag
    27241 catctgctcg ggtttactta aaacaatgga ataccaaggt ccagcgacag ctagaaaata
    27301 ttacagaacc acttgcgacg atggcttatg aagttagtgg aaactatccc catgatcaat
    27361 tagattatgc ttggaagaca ttaatgcaaa atcatcctca cgacagtatt tgcggttgtt
    27421 cagtcgattc tgtccatcgg gaaatgatac ctcgttttga aaaagcagat gaagttggaa
    27481 agtatcttgc acaagattcc ttagaacagc tgaccgcagc aatcgatacg accggattcc
    27541 caaaagacag ctttccattt gtcattgtaa atactgcagg aatggataaa actggtgaag
    27601 cagaaataac catcgaatta gaaagaaaaa gattcgctga aggaatacct gaacaactat
    27661 atcaagagtt agaaaatttg cctaaacgta agtatcatgt agaaacaaaa agtggggcca
    27721 ctataccagc tattttatct gaagaaactg tccaatttgg ttatgatttg ccgaaagacc
    27781 gtttccgtgt gccatatatg gcaagaatga tcaaagtgac tttaccatta gaaaacatgc
    27841 cagcattttc ttgggaaaca ttcgcgcttg tagaagggga agcgaaagca gaagaaaagg
    27901 aaacaatgat tcatcagagt ggacgaatca tcgaaaacgg ccctctacat ttgacgattg
    27961 aaaaaaatgg taccattaca atggaagatc gcaaaaacaa acgaaagttg aataatctgc
    28021 atatttttga agatatcggc gatataggaa atgagtatat ctttaaacaa cctttctgtg
    28081 atcagccaat cctatccagt aacaaagaaa actctgaagt aaaagtatta gtagatacac
    28141 cggaaatagc aaaaataagt attctacagg aaatggaaat ccctgtttct gcagacgagc
    28201 gtttagaaaa agaacagcag atggttgtgg aatttcgtta tcgaaaagca gaacgatcca
    28261 aagaaaaacg aatccttcaa ataaaaacaa tcatgacaat cagaaaagat agtaaaaaaa
    28321 tcgactttga aacgactctc gacaatcaga tgaaagatca tcgcttacgt gtcttgtttc
    28381 ctacaaagtt acatgtggaa catcacgaag cagatagtat ttttgaagtg gtgaagcgcc
    28441 caaaccatgt gtctaaaagt tgggaaaacc ccacgaatcc gcaacatcag caagcatttg
    28501 tcaatattca tgatgaagag tacggtgtaa cagtcggaaa ctttggtttg aatgaatatg
    28561 aagtaacaga agacggacaa atcgcagtga cgctacttcg cagtgtggga gaactaggcg
    28621 attggggata ttttccaaca ccagaagcac agtgtctagg tgagcatcgg ttcaattaca
    28681 gcattgaact acatggacca gaagaaaaat tctctactta tttgcatgca tacgcagcac
    28741 agattccttt ttccactcaa caaatcaaac atcatgaagg aacattgatt tcaaaacaac
    28801 aatatttaac gataaaaagt gaaacgtttg caattacagc tttgaaacgc agcaagttta
    28861 gtgataaagt agtagtaaga ggtttcaata tgtctagtca tttagaaaaa ttggaaataa
    28921 ctaaagacaa tggaaaaact gtcatattga atctgcttga agaaccaacg aagcaagcgg
    28981 ttgtcccaat aatccaacct tatgagattc ggacaatcgg attcgaggag gaaaacgaat
    29041 gtatggcgga atagaagcag gcggaacaaa atttgtgtgt gcggtttcag atggtggaga
    29101 aattttagaa aaattaagta tccctaccac aacaccagaa gaaacattgg atcaggtatt
    29161 tgaatttttt gatcgttatg aattgaaggg aatcgggatt ggctcattcg gcccgattgg
    29221 tatcgacaaa acaaaagata attacggata cgttttggca actcccaaaa aagggtgggc
    29281 acaattcgat tttcttggca caatcaagga acgctacaat attcctgctg cttggacaac
    29341 ggatgtcaat gctgctgcat atggggaatt gaagcaagga gcagcacaag gaaaaaatag
    29401 ttgtatttac cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca atgaagaaat
    29461 tttttcaggg atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc atccagatga
    29521 ccattatgaa ggtacttgtc cttatcataa agattgttta gaaggattag cagcaggacc
    29581 gtctattgag gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc atcctgtttg
    29641 ggctattcag gcttattata ttgctcaagc actgatcaac tatacgttga cgttagcacc
    29701 tgaaaagatt attttaggcg gtggggtaat gaatcaggat catctccttc aaaaaatccg
    29761 tcagcaattt gttgaattga tgggtggtta tatggaaaca cctcaagtaa gtgaatatat
    29821 cgttcgttgg ggactgccga atgaaagtgg gattatcggt agtttgctgt tagcagaaca
    29881 cgaacatcaa aagtaagcag acaaattaca caaaaagaac agagtcttat cattatctgc
    29941 tgataagact ctgttctttt ttatttctat tcccataatc caggtaaagc tttaccgact
    30001 attttttctt gagtaaatgg atggatcatg tgcagttcta atgcatggag catcagtcgt
    30061 tcagctgttc tattttggta gagagggtct ccaacgatcg gatgtccctt atgagctaaa
    30121 tgaactctga tctggtgtgt acgtccggtt tccaaaacac aatagacttg tgtttgttca
    30181 gaaagtactt tgttgacagt gacatgtgtc aaggcgctct gaccattttt atcatctacg
    30241 accctttttc tccgatcatg gcgatctcgt ccaattttat tggtgatggt catatctttg
    30301 gcaatttctc cccatacgat ggcttgatat ctccgataga tcattttttg ttccaacatt
    30361 cttccaagta tcggcaagac aaatggattt tttgcaaata aaacggcacc gcttgtttct
    30421 ttgtccaagc ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata agcagctaaa
    30481 tgattgagta aggtactgtt ttctgtcggt tcatttggat gagtcttcat tcctgcaggc
    30541 ttatccacta taatcaaatg ctcatcttca tataaaacac ggatgtgctt cgcatctcct
    30601 aattgaactt cttgataatt ataatctgtt tcttcgaata acaatgtgat ctggtcattg
    30661 ccctttactt catgatggaa caaagcaggt tgttgattga tccaaacgtt tttacgtata
    30721 cgcaaaaagt gtcgaacctt tctaggaact aaccattcct tttcaagcaa gtcgcgaatt
    30781 gttgtttcag gatggttcat aggtaaagtg atcgtgattt tcacaagtgt actcctttca
    30841 gtgcaattgc agactaattg taccattttt aaaacatatt taagaaaaaa cttacatttt
    30901 tctgttcatt cttatgttac tctacttgca ctaacgtaaa aaaacatatt gaactacaac
    30961 ttaagcagga agaaaacact ctttcactat ggtaaaataa gacgattgaa tcaaatagac
    31021 aggagaagaa gcaaatggat tttcaaagca ttttagaaaa gataaaagct gctttgatca
    31081 gtttttggcg ttggatcaga ccatatctcg gacaattcca tcgatggcga aagcgcatct
    31141 ggaaaaaata tcatgtgaac aaattgattt tgctgattgg tttgatctgt gtcttagtga
    31201 caagtatcta cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta aaatcaggat
    31261 taagccaatc gacagtcatt tatgataaga aaaacgaaga agctggcaca ctgtacgccc
    31321 aaaaaggaac atatgtagaa ttaaacgcga tttctcccta tatccaagat gcagtaatct
    31381 cgacagaaga taggaatttt tacgagcatc acgggtttga tatcaaaggg attgcccgtg
    31441 cagcagtccg aatggtcttg tctggcggaa catcaggggg cggaggaagt acgatcactc
    31501 agcagcttgc caaaaatgcg tatctgacat tagaccaaac gtttgatcga aaagcgaagg
    31561 aattattttt ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc ttgaccatgt
    31621 atcttaacaa tgcctatttt ggcaacgggg tatggggtgt ccaagacgct tccagaaagt
    31681 attttggagt agatgcaacg aatgttacgc tgtctgaggc tgctactttg gcaggaatgc
    31741 tgaaaggtcc tggtatttat aatccgatcg atcacccaaa aaatgcgaat gatcggaaaa
    31801 acacagtact aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa gaacaagcag
    31861 aaagccagac agatatcagc gcgtatctta atgacacata cgataattca gattctggtt
    31921 atcgttatcc ttattatttt gatgcagtga tcgatgaagc agttaacgag tatggtcttg
    31981 atgaagaaga tgtcatgaat aaaggctata aaatctatac agctttagat caggattatc
    32041 aaaagcaatt agaagctact tatcaaaaca gtgcattatt cccagcaaat gcttcagatg
    32101 gtgccatggt ccaatctgct tcagtagctt tagatccgaa tacaggtggt gtacaagcac
    32161 tagtaggacg aagaggagat catgtcttta gaggattcag ctttgctaca cagatgcgtc
    32221 gttcaccagg atcaacgatc aaaccattaa gcgtatatac accagcactg gaagctggat
    32281 acaagccttc cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg cataattttg
    32341 acgggacata ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt ttgaatttgc
    32401 ctgctgtatg gcttttgcat gagatcggtt tacaaaaagg atatgataaa gcagaagaat
    32461 tcggcctgcc tttagcaaaa tctgataagt attatggctt agcccttggt ggactggaaa
    32521 aaggagtatc tccattgatc atggcgggtg cttacagtgc atttgccaat gatggtcaga
    32581 tgtatacgcc gcatcttatc acgaaaatcg ttgattcgac tggagcggtg atcgtcgata
    32641 acacaaacaa aaaaccaaaa caggtcatat caaaggaaac agccgatgaa atgaccagca
    32701 tgcttcttgg tacattttca aatggaacag cggctgctgc gaatcctgca ggttatacga
    32761 tagcagggaa aacaggaacc acagaaacta atttcgatgc cacaaaagca aatgaccaat
    32821 ggatgatcgg ttacacgcca gacgtagtga tttcgacatg gatgggttac gaagaatcca
    32881 gcaagctgca ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc ttcaaatcag
    32941 cggcagaagg catccttccg tatacttcaa aaacaggatt tagtgtagca gatgcctatg
    33001 ccacaggagg acaagtggta gctgcagatc aagttggaaa cacaggaaat tctaatggag
    33061 agtctggaaa ttggcaagat aacttgaatc aatatggtga acaagcaaaa gaagggctga
    33121 aaaacttcgg cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg gctaaagacc
    33181 tctggcggaa atatcaagga gagtagcccc tttgaaaacg aataacccca tgttacaata
    33241 gagagtaact actaaaagaa aagagggcat aacatgtcaa acatctacga tagcgcgaat
    33301 caaatcgaac gtgagatccg tgaattacca gaattcaaag cgttgaaaga agcgtacgag
    33361 aaagtacaag caaacgaaga agcacacaaa ctgttcaaag acttccaagc gatgcaaatc
    33421 gaattacaag aaaaacaaat gagtggacaa gagttcagcg acgaagatgc aatgaaagca
    33481 caagaaatgg ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat gcaaaaagag
    33541 caaggcttca gtacaatcat caatgacttg aaccgtatca tcatgacacc agttcgcgac
    33601 ctttacagcg aataaaacta ttttatgaaa caacagaggg tgtgaaagag acgttcggcc
    33661 ttgagcgatg acaaaattgc ttcttattgc cgataggctg tctcttgaac accatttacc
    33721 aagaggtcgt gagcctaccg tttaactctg agttacaagg aacaatttca aaaaacagct
    33781 tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta
    33841 ttcagaaata ggagctacga caaaagtctt gtcatagctc ctattttttg tcattcattt
    33901 ttcaagcggt agaaatagcc tttttgagct aacagttttt cgtaagtacc tatttcaacg
    33961 atttttcctt catcgatcac aacgatttta tctgcctctt tgatcgttga aagccgatga
    34021 gccacaataa aagtagtggg tgtttctgtc aggtaattca gggcttgctg gatttttttc
    34081 tctgactggt tatctaaagc tgaggttgct tcatctagca aaatgatttt tggctgacgt
    34141 atcaacgctc gtgcaatgga gatacattgc ttttggccac cagacagctt gttgccatgt
    34201 tcgcctacta aggtatccag accatcagga agctggtcaa tcagtgaaga aagttgcgca
    34261 gcttcgatga cttcatctaa acgttctttt gatacgtttt tcaagccata agtgatgttc
    34321 tctttgatcg aagcggagaa caacaaagtg gtttgaggaa caacggctaa ataatttcgc
    34381 acacttcgca tatccagttc atccaaaggc tgatcatcta gaaaaatttt tcctgaagtc
    34441 ggctggataa atccaatcaa taagttaagg agtgtagatt ttccagagcc agatggccca
    34501 acaaaagcaa ttgtttctcc agctttcacc tctagagaaa aatgatcgat aatcggtttg
    34561 agtgagtccg gataaagaaa agaaacatcc tcaaaacgaa aatttccatc caaatgttgg
    34621 atttttttct taccgtgata agtttctaca tcgccagcaa tcaacagttc gccaatagaa
    34681 gtgatagatt ccatgccttt tgaaatcgta ggaatcagcg tgatcattcc agaaacggaa
    34741 ttgacgatcg tcgtgaaata cgtctgatac atcacgacat cgccgggttg gatgcgccct
    34801 tgtaaagcta atatcccagt gaaactcaaa caaatcagct gaaatatctg aaaactagcc
    34861 cagctgactg atccgaaaac agattgcagg atatctaagt ggaatccttt ttcatagatt
    34921 tgagtaaaat gcttatttaa gcgagttgtt tcgtgttttt ctaacccatg agctcgagca
    34981 atcggtgcta attcgatcac ttcgatcact tttgcagaag tctcttccat ttcttgcctg
    35041 aagtctgagt tggactgtct gatcctttta cggaaaaaga gagtaacgaa tgcggcgaga
    35101 ggaacagtca ataagaaaaa catgaaaaca acaggacttt tataaagcgt gatacctaaa
    35161 gctacgaata aattgacagc gatatttaat ccgctgacaa atacttgaga ggacaacgtt
    35221 tggatagctt ctacatcacg gatgatcttt gactggattc gtccagattg caattctttt
    35281 tgataaggaa tagatagttc ttggattttc ttgataaggg tcgctcttag tccagcttct
    35341 acttgacgga tcgaagtgct atggtatttt acatgaaaat aattcgtcaa tatattttga
    35401 ccaatcagac aagccatcaa aaaagcgttg aaataaatgg cctgcaactg agaagaatct
    35461 ttcgatgaag ctgcattgat tacattggca atagcgatcg gcattaacca agatggaaga
    35521 tgtttgataa taaaaaagag tcctgacatg gcaaaacgcc aataatttcc ctgataaaga
    35581 tgcagcaatg tctgaaaagc atttgcatga tatttttgat aacttttctg tattcgttcc
    35641 tccataatct tctccttaac aatcttggta aataaactgc aagtgttcta ttgcttcagg
    35701 aacataaatc tcaggagtag cttcaagagt ggcgtacatt agaggacgct catatttgat
    35761 ataatgcagt accgggcgga aatctaaaca gcccttgcca acaggtacca tgcggatctt
    35821 tccattttcc atgatatagt ctttgagatg aagcgctgta atataaccat gtaaattgtc
    35881 caaggcacct cggatcactt tttcctgatc agcatgattt tctttatgca tcaaattcgc
    35941 acaatctaaa atgattttta aatttggtga agctacttca tcgattaaac gtttagctaa
    36001 ctgatagtta tagattggat gattgatccc tgcttcaaca gcaacagtta cgccaaaatt
    36061 ttctgcatga gcgactaatt caatgataga atttctggcg atctgataag cttcttcagt
    36121 aaaattttct tttgtgtaac cgacagagac actaccggtt tccgtagcaa tcatcttggc
    36181 ttgataggca gagcacagag caatttgctg tttgaaagtt gccaatgctt gtgcacgaac
    36241 ttcttgattt ttgctggata ggttgatata gcatcctaaa atggaaagtt cgatattgtt
    36301 tttttggaag tattcaccaa aataggcagc actcccaagt gtcatgtcag ttaatttaag
    36361 ttgttcagga aatgcttttt gtggtgcaaa ttggatatgg gacaagtgaa gctgtgatac
    36421 tttttgaacg atttcttcac gatttcgaac atctaaatcg tgggcacgga tgcctaaatt
    36481 taatggaaac atctcaaact cctttttgta aataattagc aagaggcaga cgggattctt
    36541 ttatcaattc aacaattatt cgggcaactt tatcagcgcc ttctggacta aagtgggtat
    36601 tgtcttttag tccttctggg taatttttac aagtatcagg cataagatgc aggtagaatt
    36661 tttttgtctc ttcttttgag aaggtctgga acaattcttg tgatcttgag aacacatcta
    36721 aaagaggaat atggtgtttc tcagcaaatg cacgcatcgc ttttggatag tctccaagta
    36781 tatctggatt caatgttccg ttttctaagt aatctcgtcg cgtcacagat gtcagtaaga
    36841 ccatttggac gttcgctttt tttgcgcatt caaaaaatat ctgcagattt tttaaatagc
    36901 taccgaaggg aagcgtacct ctttcttctt ggattttctg gtcgttatgt ccaaattgta
    36961 caaataagaa atcattcggc tgaaactgtg aagctagacg ggctaacctt ccttcctgga
    37021 taaatgattt ggtgctgcgt ccattctttg cttggttatt gatctggatc tcgggtgaaa
    37081 aataagcaga gattttttct ccccaacctg tctcgggacg cttgttttca tctttttctg
    37141 ccgcagtaga atcaccggca atatggatag taggcatgtt atctcttctt tcataaaggt
    37201 ttataggcaa tgaagcagaa cagcaagaga agaagacgga gcaa
//
LOCUS       DAZUMT010000015        34954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000015 DAZUMT010000000
VERSION     DAZUMT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.015"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACG8FM_000693"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACG8FM_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS5093379.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACG8FM_000694"
     CDS             complement(2017..2919)
                     /locus_tag="ACG8FM_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5093380.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACG8FM_000695"
     CDS             complement(2916..3608)
                     /locus_tag="ACG8FM_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS5093381.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACG8FM_000696"
     CDS             complement(3651..4292)
                     /locus_tag="ACG8FM_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS5093382.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="ACG8FM_000697"
     CDS             complement(4330..4644)
                     /locus_tag="ACG8FM_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093383.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACG8FM_000698"
     CDS             complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACG8FM_000698"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS5093384.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACG8FM_000699"
     CDS             complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACG8FM_000699"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS5093385.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5981..6913)
                     /locus_tag="ACG8FM_000700"
     CDS             complement(5981..6913)
                     /locus_tag="ACG8FM_000700"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS5093386.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="ACG8FM_000701"
     CDS             complement(6910..7686)
                     /locus_tag="ACG8FM_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS5093387.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACG8FM_000702"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACG8FM_000702"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS5093388.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /locus_tag="ACG8FM_000703"
     CDS             complement(10930..12006)
                     /locus_tag="ACG8FM_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS5093389.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="ACG8FM_000704"
     CDS             complement(12011..13291)
                     /locus_tag="ACG8FM_000704"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS5093390.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="ACG8FM_000705"
     CDS             complement(13310..14236)
                     /locus_tag="ACG8FM_000705"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS5093391.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="ACG8FM_000706"
     CDS             complement(14249..15532)
                     /locus_tag="ACG8FM_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS5093392.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACG8FM_000707"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACG8FM_000707"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS5093393.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="ACG8FM_000708"
     CDS             complement(16320..17231)
                     /locus_tag="ACG8FM_000708"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5093394.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACG8FM_000709"
     CDS             complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACG8FM_000709"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS5093395.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="ACG8FM_000710"
     CDS             complement(17697..18395)
                     /locus_tag="ACG8FM_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS5093396.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="ACG8FM_000711"
     CDS             complement(18382..18768)
                     /locus_tag="ACG8FM_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093397.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="ACG8FM_000712"
     CDS             complement(18915..20585)
                     /locus_tag="ACG8FM_000712"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS5093398.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="ACG8FM_000713"
     CDS             20768..21001
                     /locus_tag="ACG8FM_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5093399.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="ACG8FM_000714"
     CDS             complement(21100..21531)
                     /locus_tag="ACG8FM_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS5093400.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..22023
                     /locus_tag="ACG8FM_000715"
     CDS             21610..22023
                     /locus_tag="ACG8FM_000715"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS5093401.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22061..23209)
                     /locus_tag="ACG8FM_000716"
     CDS             complement(22061..23209)
                     /locus_tag="ACG8FM_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5093402.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23331..23927)
                     /locus_tag="ACG8FM_000717"
     CDS             complement(23331..23927)
                     /locus_tag="ACG8FM_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5093403.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACG8FM_000718"
     CDS             complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACG8FM_000718"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS5093404.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27157..27588
                     /locus_tag="ACG8FM_000719"
     CDS             27157..27588
                     /locus_tag="ACG8FM_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5093405.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27633..28982)
                     /locus_tag="ACG8FM_000720"
     CDS             complement(27633..28982)
                     /locus_tag="ACG8FM_000720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5093406.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29052..30215)
                     /locus_tag="ACG8FM_000721"
     CDS             complement(29052..30215)
                     /locus_tag="ACG8FM_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5093407.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30344..30667
                     /locus_tag="ACG8FM_000722"
     CDS             30344..30667
                     /locus_tag="ACG8FM_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093408.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30850..32199)
                     /locus_tag="ACG8FM_000723"
     CDS             complement(30850..32199)
                     /locus_tag="ACG8FM_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5093409.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32268..33221)
                     /locus_tag="ACG8FM_000724"
     CDS             complement(32268..33221)
                     /locus_tag="ACG8FM_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS5093410.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33323..34513)
                     /locus_tag="ACG8FM_000725"
     CDS             complement(33323..34513)
                     /locus_tag="ACG8FM_000725"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS5093411.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34713..>34954)
                     /locus_tag="ACG8FM_000726"
     CDS             complement(34713..>34954)
                     /locus_tag="ACG8FM_000726"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093412.1"
                     /translation="KNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFL
                     LVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9948 a         7640 c         6119 g        11247 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    22021 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    22081 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    22141 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    22201 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    22261 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    22321 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    22381 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    22441 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    22501 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    22561 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    22621 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    22681 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    22741 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    22801 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    22861 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    22921 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    22981 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    23041 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    23101 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    23161 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    23221 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    23281 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    23341 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    23401 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    23461 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    23521 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    23581 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    23641 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    23701 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    23761 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    23821 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    23881 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    23941 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    24001 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    24061 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    24121 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    24181 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    24241 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    24301 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    24361 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    24421 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    24481 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    24541 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    24601 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    24661 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    24721 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    24781 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    24841 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    24901 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    24961 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    25021 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    25081 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    25141 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    25201 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    25261 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    25321 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    25381 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    25441 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    25501 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    25561 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    25621 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    25681 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    25741 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    25801 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    25861 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    25921 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    25981 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    26041 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    26101 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    26161 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    26221 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    26281 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    26341 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    26401 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    26461 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    26521 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    26581 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    26641 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    26701 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    26761 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    26821 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    26881 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    26941 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    27001 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    27061 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    27121 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    27181 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    27241 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    27301 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    27361 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    27421 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    27481 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    27541 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    27601 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    27661 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    27721 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    27781 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    27841 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    27901 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    27961 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    28021 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    28081 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    28141 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    28201 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    28261 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    28321 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    28381 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    28441 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    28501 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    28561 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    28621 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    28681 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    28741 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    28801 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    28861 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    28921 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    28981 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    29041 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    29101 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    29161 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    29221 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    29281 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    29341 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    29401 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    29461 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    29521 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    29581 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    29641 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    29701 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    29761 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    29821 aaataatatc ttaaataaat gccaaacggt atgagtaata aaacattaaa agctggttcc
    29881 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    29941 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    30001 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    30061 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    30121 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    30181 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    30241 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    30301 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    30361 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    30421 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    30481 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    30541 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    30601 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    30661 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    30721 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    30781 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    30841 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    30901 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    30961 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtcgaaacaa
    31021 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    31081 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    31141 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    31201 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc ataacataag ggagacgcat
    31261 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    31321 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    31381 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    31441 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    31501 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    31561 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    31621 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    31681 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    31741 gatcaacagg atcgatacag cagctgtaac agctacggga acaatcattg actgggaaag
    31801 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    31861 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    31921 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    31981 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    32041 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    32101 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    32161 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    32221 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    32281 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    32341 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    32401 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    32461 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    32521 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    32581 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    32641 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    32701 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    32761 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    32821 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    32881 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    32941 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    33001 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    33061 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    33121 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    33181 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    33241 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    33301 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    33361 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    33421 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    33481 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    33541 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    33601 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    33661 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    33721 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    33781 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    33841 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    33901 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    33961 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    34021 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    34081 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    34141 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    34201 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    34261 aaccattgat cgttcggact gctcctgctc ctacaccata agtttcggtt gcttcaatac
    34321 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    34381 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    34441 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    34501 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    34561 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    34621 atgagaatta tttaaccaca ttttttattt acagtatctt tttactttca aatgacttat
    34681 atagtatgaa caattacctt ttagataatg atctatttta ctctacctgg gcagataaat
    34741 acaacacaac cattgataga agccctttcg ttaaaatcaa ttgtattctg aatatttaat
    34801 ttatttattg catagaccaa taaaaataca taaaatttcc atcttcgttt atcagcaatt
    34861 tttaatttta ctctctgaat atttcttaaa agttttactt gttgaattcg aaaaaaggac
    34921 tgtgacaaaa gtcttgtcac agtcctattc ttcc
//
LOCUS       DAZUMT010000016        34688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000016 DAZUMT010000000
VERSION     DAZUMT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.016"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG8FM_000727"
     CDS             591..1280
                     /locus_tag="ACG8FM_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5093413.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG8FM_000728"
     CDS             1267..2475
                     /locus_tag="ACG8FM_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5093414.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG8FM_000729"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG8FM_000729"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS5093415.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8FM_000730"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8FM_000730"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS5093416.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG8FM_000731"
     CDS             complement(6578..7273)
                     /locus_tag="ACG8FM_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS5093417.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8FM_000732"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8FM_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS5093418.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG8FM_000733"
     CDS             complement(8472..8663)
                     /locus_tag="ACG8FM_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS5093419.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG8FM_000734"
     CDS             complement(8684..9574)
                     /locus_tag="ACG8FM_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS5093420.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG8FM_000735"
     CDS             complement(9561..10328)
                     /locus_tag="ACG8FM_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5093421.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8FM_000736"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8FM_000736"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS5093422.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8FM_000737"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8FM_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS5093423.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8FM_000738"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8FM_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS5093424.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG8FM_000739"
     CDS             14899..15282
                     /locus_tag="ACG8FM_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093425.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8FM_000740"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8FM_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS5093426.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG8FM_000741"
     CDS             complement(16226..17044)
                     /locus_tag="ACG8FM_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS5093427.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8FM_000742"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8FM_000742"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5093428.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8FM_000743"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8FM_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS5093429.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18173..19507
                     /gene="dnaA"
                     /locus_tag="ACG8FM_000744"
     CDS             18173..19507
                     /gene="dnaA"
                     /locus_tag="ACG8FM_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS5093430.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19705..20835
                     /gene="dnaN"
                     /locus_tag="ACG8FM_000745"
     CDS             19705..20835
                     /gene="dnaN"
                     /locus_tag="ACG8FM_000745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS5093431.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21064..21306
                     /gene="yaaA"
                     /locus_tag="ACG8FM_000746"
     CDS             21064..21306
                     /gene="yaaA"
                     /locus_tag="ACG8FM_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS5093432.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21293..22417
                     /gene="recF"
                     /locus_tag="ACG8FM_000747"
     CDS             21293..22417
                     /gene="recF"
                     /locus_tag="ACG8FM_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS5093433.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22414..24360
                     /gene="gyrB"
                     /locus_tag="ACG8FM_000748"
     CDS             22414..24360
                     /gene="gyrB"
                     /locus_tag="ACG8FM_000748"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS5093434.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24380..26851
                     /gene="gyrA"
                     /locus_tag="ACG8FM_000749"
     CDS             24380..26851
                     /gene="gyrA"
                     /locus_tag="ACG8FM_000749"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS5093435.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27087..27386
                     /gene="rpsF"
                     /locus_tag="ACG8FM_000750"
     CDS             27087..27386
                     /gene="rpsF"
                     /locus_tag="ACG8FM_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS5093436.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27436..27948
                     /gene="ssb"
                     /locus_tag="ACG8FM_000751"
     CDS             27436..27948
                     /gene="ssb"
                     /locus_tag="ACG8FM_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5093437.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27974..28210
                     /gene="rpsR"
                     /locus_tag="ACG8FM_000752"
     CDS             27974..28210
                     /gene="rpsR"
                     /locus_tag="ACG8FM_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS5093438.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28353..30326
                     /locus_tag="ACG8FM_000753"
     CDS             28353..30326
                     /locus_tag="ACG8FM_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS5093439.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30332..30784
                     /gene="rplI"
                     /locus_tag="ACG8FM_000754"
     CDS             30332..30784
                     /gene="rplI"
                     /locus_tag="ACG8FM_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS5093440.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31058..32425
                     /gene="dnaB"
                     /locus_tag="ACG8FM_000755"
     CDS             31058..32425
                     /gene="dnaB"
                     /locus_tag="ACG8FM_000755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS5093441.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33205..34497
                     /locus_tag="ACG8FM_000756"
     CDS             33205..34497
                     /locus_tag="ACG8FM_000756"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS5093442.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10563 a         6737 c         6861 g        10527 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgcggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gagtcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa tggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggtgatttc ctgtgtattc cgtctcctaa ctattggtat atctatattt
    17941 tttatccaca gatgcaagag gaaatctatt gatttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    18061 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    18121 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    18181 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18241 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18301 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18361 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18421 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18481 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18541 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18601 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18661 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18721 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18781 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18841 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18901 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgcctcgttt ctcgctttgc
    18961 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    19021 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    19081 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    19141 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    19201 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19261 ttatcacatc caattgaaag atatcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19321 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19381 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19441 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19501 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19561 aaaagttatg cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19621 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19681 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19741 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19801 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19861 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19921 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19981 tttactttag aagttttaga aaataaacaa gtagcgatta cttctggaaa agcgaatttt
    20041 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    20101 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    20161 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    20221 ttattggctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20281 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20341 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20401 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatcctga taccaatcgt
    20461 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20521 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20581 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20641 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20701 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20761 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20821 gtacgtacaa attaacaatg attttcattg ttcttgaaaa tttaattgaa aagaaataga
    20881 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20941 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    21001 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    21061 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    21121 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    21181 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21241 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21301 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21361 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21421 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21481 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21541 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacaaaaaaa
    21601 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21661 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21721 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21781 tttaaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21841 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21901 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21961 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    22021 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    22081 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    22141 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    22201 taaagaagag acgggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22261 tgatagtcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22321 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22381 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22441 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22501 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22561 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22621 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22681 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22741 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22801 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22861 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22921 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22981 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    23041 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    23101 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    23161 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    23221 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23281 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23341 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23401 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23461 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23521 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23581 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23641 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23701 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23761 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23821 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23881 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23941 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    24001 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    24061 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    24121 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    24181 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24241 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24301 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24361 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24421 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24481 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24541 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24601 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24661 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24721 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24781 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24841 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24901 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24961 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    25021 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    25081 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    25141 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    25201 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25261 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25321 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25381 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25441 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25501 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25561 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25621 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25681 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25741 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25801 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25861 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25921 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25981 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    26041 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    26101 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    26161 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    26221 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26281 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26341 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26401 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26461 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26521 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26581 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26641 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26701 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26761 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26821 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26881 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26941 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    27001 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    27061 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    27121 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    27181 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27241 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27301 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27361 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27421 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27481 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27541 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27601 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27661 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27721 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27781 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27841 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27901 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27961 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    28021 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    28081 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    28141 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    28201 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28261 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28321 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28381 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28441 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28501 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28561 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28621 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28681 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28741 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28801 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28861 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28921 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28981 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    29041 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    29101 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    29161 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    29221 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29281 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29341 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29401 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29461 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29521 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29581 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29641 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29701 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29761 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29821 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29881 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29941 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    30001 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    30061 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    30121 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    30181 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30241 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30301 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30361 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30421 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30481 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30541 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30601 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30661 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30721 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30781 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30841 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30901 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30961 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    31021 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    31081 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    31141 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    31201 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31261 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31321 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31381 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31441 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31501 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31561 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31621 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31681 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31741 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31801 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31861 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31921 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31981 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    32041 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    32101 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    32161 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    32221 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32281 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32341 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32401 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32461 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32521 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32581 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32641 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32701 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32761 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32821 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32881 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32941 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    33001 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    33061 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    33121 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    33181 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33241 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33301 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33361 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33421 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33481 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33541 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33601 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33661 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33721 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33781 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33841 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33901 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33961 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    34021 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    34081 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    34141 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    34201 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34261 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34321 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34381 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34441 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34501 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34561 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34621 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34681 tttccgaa
//
LOCUS       DAZUMT010000017        34556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000017 DAZUMT010000000
VERSION     DAZUMT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.017"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..863)
                     /locus_tag="ACG8FM_000757"
     CDS             complement(<1..863)
                     /locus_tag="ACG8FM_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5093443.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRK"
     gene            1016..2476
                     /gene="rhaB"
                     /locus_tag="ACG8FM_000758"
     CDS             1016..2476
                     /gene="rhaB"
                     /locus_tag="ACG8FM_000758"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS5093444.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            2473..3729
                     /gene="rhaA"
                     /locus_tag="ACG8FM_000759"
     CDS             2473..3729
                     /gene="rhaA"
                     /locus_tag="ACG8FM_000759"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS5093445.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            3743..4588
                     /gene="rhaD"
                     /locus_tag="ACG8FM_000760"
     CDS             3743..4588
                     /gene="rhaD"
                     /locus_tag="ACG8FM_000760"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS5093446.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            4590..4901
                     /gene="rhaM"
                     /locus_tag="ACG8FM_000761"
     CDS             4590..4901
                     /gene="rhaM"
                     /locus_tag="ACG8FM_000761"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS5093447.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            4983..6134
                     /gene="fucO"
                     /locus_tag="ACG8FM_000762"
     CDS             4983..6134
                     /gene="fucO"
                     /locus_tag="ACG8FM_000762"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS5093448.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            6261..7019
                     /locus_tag="ACG8FM_000763"
     CDS             6261..7019
                     /locus_tag="ACG8FM_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS5093449.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            7119..7763
                     /locus_tag="ACG8FM_000764"
     CDS             7119..7763
                     /locus_tag="ACG8FM_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5093450.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            7770..8777
                     /locus_tag="ACG8FM_000765"
     CDS             7770..8777
                     /locus_tag="ACG8FM_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5093451.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            8793..9635
                     /gene="kduI"
                     /locus_tag="ACG8FM_000766"
     CDS             8793..9635
                     /gene="kduI"
                     /locus_tag="ACG8FM_000766"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS5093452.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            9655..10458
                     /locus_tag="ACG8FM_000767"
     CDS             9655..10458
                     /locus_tag="ACG8FM_000767"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS5093453.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(10707..11771)
                     /gene="fni"
                     /locus_tag="ACG8FM_000768"
     CDS             complement(10707..11771)
                     /gene="fni"
                     /locus_tag="ACG8FM_000768"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS5093454.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(11768..12853)
                     /locus_tag="ACG8FM_000769"
     CDS             complement(11768..12853)
                     /locus_tag="ACG8FM_000769"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS5093455.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(12866..13843)
                     /gene="mvaD"
                     /locus_tag="ACG8FM_000770"
     CDS             complement(12866..13843)
                     /gene="mvaD"
                     /locus_tag="ACG8FM_000770"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS5093456.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(13836..14780)
                     /gene="mvk"
                     /locus_tag="ACG8FM_000771"
     CDS             complement(13836..14780)
                     /gene="mvk"
                     /locus_tag="ACG8FM_000771"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS5093457.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            15096..15755
                     /locus_tag="ACG8FM_000772"
     CDS             15096..15755
                     /locus_tag="ACG8FM_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5093458.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            15840..16745
                     /locus_tag="ACG8FM_000773"
     CDS             15840..16745
                     /locus_tag="ACG8FM_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5093459.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(16747..17379)
                     /locus_tag="ACG8FM_000774"
     CDS             complement(16747..17379)
                     /locus_tag="ACG8FM_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5093460.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    17530..17655
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17699..18223
                     /gene="infC"
                     /locus_tag="ACG8FM_000775"
     CDS             17699..18223
                     /gene="infC"
                     /locus_tag="ACG8FM_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS5093461.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            18295..18495
                     /gene="rpmI"
                     /locus_tag="ACG8FM_000776"
     CDS             18295..18495
                     /gene="rpmI"
                     /locus_tag="ACG8FM_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS5093462.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            18548..18907
                     /gene="rplT"
                     /locus_tag="ACG8FM_000777"
     CDS             18548..18907
                     /gene="rplT"
                     /locus_tag="ACG8FM_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS5093463.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            19159..20496
                     /locus_tag="ACG8FM_000778"
     CDS             19159..20496
                     /locus_tag="ACG8FM_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS5093464.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            20544..22673
                     /locus_tag="ACG8FM_000779"
     CDS             20544..22673
                     /locus_tag="ACG8FM_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS5093465.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            22648..23337
                     /locus_tag="ACG8FM_000780"
     CDS             22648..23337
                     /locus_tag="ACG8FM_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093466.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(23745..24358)
                     /locus_tag="ACG8FM_000781"
                     /pseudo
     CDS             complement(23745..24358)
                     /locus_tag="ACG8FM_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(24494..24688)
                     /locus_tag="ACG8FM_000782"
     CDS             complement(24494..24688)
                     /locus_tag="ACG8FM_000782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093467.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(24738..25178)
                     /locus_tag="ACG8FM_000783"
     CDS             complement(24738..25178)
                     /locus_tag="ACG8FM_000783"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS5093468.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(25182..26027)
                     /locus_tag="ACG8FM_000784"
     CDS             complement(25182..26027)
                     /locus_tag="ACG8FM_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS5093469.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            26176..27594
                     /gene="pepV"
                     /locus_tag="ACG8FM_000785"
     CDS             26176..27594
                     /gene="pepV"
                     /locus_tag="ACG8FM_000785"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS5093470.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(27651..28598)
                     /locus_tag="ACG8FM_000786"
     CDS             complement(27651..28598)
                     /locus_tag="ACG8FM_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5093471.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(28821..29171)
                     /locus_tag="ACG8FM_000787"
     CDS             complement(28821..29171)
                     /locus_tag="ACG8FM_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5093472.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            29371..30450
                     /gene="pepA"
                     /locus_tag="ACG8FM_000788"
     CDS             29371..30450
                     /gene="pepA"
                     /locus_tag="ACG8FM_000788"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS5093473.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            30594..30914
                     /locus_tag="ACG8FM_000789"
     CDS             30594..30914
                     /locus_tag="ACG8FM_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5093474.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            30936..31400
                     /locus_tag="ACG8FM_000790"
     CDS             30936..31400
                     /locus_tag="ACG8FM_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5093475.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            31605..32210
                     /gene="ytpR"
                     /locus_tag="ACG8FM_000791"
     CDS             31605..32210
                     /gene="ytpR"
                     /locus_tag="ACG8FM_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS5093476.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(32249..33538)
                     /locus_tag="ACG8FM_000792"
     CDS             complement(32249..33538)
                     /locus_tag="ACG8FM_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS5093477.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            33966..34445
                     /gene="rlmH"
                     /locus_tag="ACG8FM_000793"
     CDS             33966..34445
                     /gene="rlmH"
                     /locus_tag="ACG8FM_000793"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS5093478.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        10901 a         6392 c         7336 g         9927 t
ORIGIN      
        1 aatttacgaa agtctctcgg cgatagtcct gtttttgctt tgaacaggcg gctaaagtaa
       61 gcaaaattct caatccctat ttcttcggaa atcattcgaa ccgtaaattc tgtataccga
      121 agaaggtctt tagcgtaaga aagttttttg ctgatcaaat aatcgattgg tggagaaccg
      181 ataaatttgg aaaaatcttt gctcagctga tatttattca ataagaattg ttgttctaat
      241 tgatcaagtg taatcgtttc tttgaaatgt tcgtctaagt attgcttcag tttgtaaacg
      301 taggcgggaa tatcctcttc ttcgaaatct agctgatact tttgcaaaat cagttcattc
      361 agcaactcgt ggatagctac agaggactgg aaatcggtac gggcattcgt gccttgttgc
      421 atgtggatca attctgtgat caaatggtgg atcttgtttt ctgatggatc ccctgttgaa
      481 tggaaggtgt tatggccgtc ttttgtgaac tcctgataaa atgattggac gttctgacca
      541 ttcaaatgga tccaatccat ttcccatggt tcttcgctga tcgtttgata acgctgatat
      601 tcttggcagt caataaaaaa taagtctcca gcttttagct gttgctgatt atcattatat
      661 gtaagcgaac ctttgccaga caaagtaaac tttatcaagt aagaaggtaa atgtgctcgt
      721 tcagtatagt aagggattgg tgctttgaag taaccgattt cttggatata aaaaaataaa
      781 ttcctgcttc tttgtgtagg cgtcaagatc actctttttg agtcttccga ccatgagtgg
      841 ttggcttttt cccagtaatc catgtaaaca ctcccaactt gtcaagataa tgcaattata
      901 taccaaaata gtgtgaagca aaatataaga ttctgtatta tgatagcatt gttaataaat
      961 gataacgctt tattaataaa gtgctaatca acaataaagt aaaagaggtg gagaaatgaa
     1021 tccaacattt gttgccatcg atatcggtgc atcaagcgga cgactaatga aaagtacgag
     1081 ggatgcaaaa ggttctttga tcttggaaga agtacaccgg ttcaaaaacg gctttcggaa
     1141 caaagacggt cacgatcgct ggaagatcga ttatcttatc aaagagattt tgattggatt
     1201 tgaaaaatta aagcagacag ggattgatcg cctttttgcg gggatcgata catgggcagt
     1261 cgattactgt ctgattgatg aacaagggga tcggataaat gatccgattg cttatcggga
     1321 caagcgtacc aatggagcag tagaacagtt ttctgaaatc tattctctag ataagttgta
     1381 tgagcagaca gggattcaga tccagccttt caataccttg ttccaattgt ttgctgaaga
     1441 aaaagaaaat ttggcaaaag cacataaatt gttactgata cctgattatt tagggtatgt
     1501 ctttactggt aacatggtga cggagaaaac taatgcatct actatgcagc tgctgaatgc
     1561 agggacaaaa gaatgggaaa tagaacttct tgaaaaaatc ggtgtctcga aggaattatt
     1621 tccaccattg acagagccgg gaacgatact aggagatctg caaaaagaac aattttcaaa
     1681 atatgatttg ccagatgtga cctttatcac agtagcaagt catgatacgg cgtcagctgt
     1741 attaggaacg ccaggtcaag gagaacgttg gggttatatc agcagtggga catggtcgct
     1801 tttagggatt gaaacaaaga tcgccaatgt ttctcgcaat gctttttctg aaaattatac
     1861 gaatgaatgg ggtgcccata acacggtgcg ttttttgaaa aacatcatgg ggatgtggtt
     1921 gatccaggaa gttgcccgaa tgcaagagta cgagtacagt tatgcggaat tggcagagct
     1981 tgctgcaaaa gaaaaaccgc tgcagcaagt gatggaaatc aatgaccctc gatttttgaa
     2041 tcccccaaat atgatagaag agattcagca atactgtttt gaaacagagc agccagtccc
     2101 tgtctcatcg ggagctatcg ctcgttgtgt gtatgataat cttgctcttt gttacgcaca
     2161 agagctgatc aaagtagaac acttaactgg aacgaaattg gaaaagctgc acatcgtagg
     2221 gggcggatcg aataatcgtc tactgaatca attgacagca gattatgccg gaatcattgt
     2281 agaagcagga cctagtgaag ccacagcaat cgggaatgtt gccatgcaaa tgctcagtac
     2341 aggtcagttt tcgaatatag aacaagcaag aaatgtaatc aaacagtcgt ttccatgtga
     2401 aactttttac ccagatgaaa ataagcaaaa cacgatggaa tggtacgtga ataaaatcaa
     2461 aggagtaatc gtatgaacac gattgaaaga aattacgaac aagcaaaaga aaaatatgca
     2521 actatcggtg tagatacaga tgctgtacta gaaaagatac aggacatcaa aatttctatg
     2581 cattgctggc aaggagatga tgtcaaaggt ttcttgacac cggatggcga attgaccggt
     2641 ggaatcatgg cgacagggaa ttttccagga gccgcacgaa cacctgaaga gcttagacaa
     2701 gatctggaga aggcttattc gttgataccg ggcaaacaca aattgaacct tcatgctatc
     2761 tatttggata cagaagaatc tgtcgatcta aatgaaattg agccgaaaca ttttgaaaaa
     2821 tgggtagagt gggcaaaaaa agaagaaatc ggtcttgact tcaatccgac tttcttttcg
     2881 catccaatga tgaaagacgg ctttacattg gctcatccag ataaagaagt tcgtgaatac
     2941 tggatcgaac acgggaaaag aagccgcaaa atcgcggagt actttgggaa agaaactggg
     3001 caaacaagta tcaataactt ttgggtacct gatgggatga aagataatcc gattgatcgt
     3061 tttaccccac gggaacgttt aatggaatca ctcgatgaaa tttttacaga agagttagat
     3121 gaacggtatg tacaagatgc agtagaaagc aaactatttg gtttaggcgc tgaagcttat
     3181 acagtcggtt cccatgaatt ttatatgggt tacgggttga ctcgtgacaa acttgtttgt
     3241 ctagatgctg gacattttca tccaacagaa gtgatttcca ataaattatc ttcattagct
     3301 ttattcagca aaggaatcat gttgcatgtt tcccgtccag tgcgttggga ttcagaccat
     3361 gtggttttga tggatgatga attgcaggaa atcgctaaag aattagtccg taacgaattg
     3421 ttggaaaaaa caaatatcgg tctggatttc tttgatgcaa cgatcaaccg tatcgctgct
     3481 tgggtcatcg gtacgagaaa tacacaaaaa gcactgctga aagctatgct ggaaccagtt
     3541 gagcgattga aagaaatgga gctggctttt gactttactt caagaatggc ttatacggaa
     3601 gaattgaaag atttcccgta tgccgatgta tggaattatt tctgttacaa aaatcaagtt
     3661 ccagttggtt tggattggct ggaagaagtc caggaatatc aaaaagacgt gttggaatta
     3721 agaaaatagg agtgtggcta tattgaaaaa aatagatatt ttacaagcgc cttatgtgaa
     3781 agaaatgatc gaaacgacgt cgaatcttta tcgcttggga tgggatgaaa gaaatggcgg
     3841 gaatatttct tatttactaa aagaagaaga gatcacaccg ttcttggatc ctgaaaaagt
     3901 gatccgccgc atctcgatga tttttgatgc cagccgttta gcaggatgct attttattgt
     3961 aactggttca ggaagatatt ttaaaaatgt agcgagcgaa cctgccgaga atcttggaat
     4021 cgttcgagtt gctgaagatg gcaaaacact ggaacttttg tgggggctgg aaaacgaatg
     4081 ccttcctaca agcgagttgc caagtcattt catgagtcat atcgcccgtt tagaagtaga
     4141 cccagataat cggatcgtta tgcattgcca tgctagtcat ctattagcga tgagttttac
     4201 gcatgaatta gacgaacgtt cctttagtcg tacgctatgg caaatgtgta cggaatgctt
     4261 agttgttttt ccagaaggaa tttccatcat tccgtggatg gttccgggaa caaatgagat
     4321 tggggaagca acagctgaaa aaatgaaaga aactcgattg gttttatggc cacaacatgg
     4381 aatctacggg tcaggaaaag acatggatga agtctttggt ttgatcgaga cagcagaaaa
     4441 ggcagcagaa gtctatacgt acgtcaaagc gcaaggacca atcttgcaaa caatcacgga
     4501 cgaaaacctg tggcatttag cagatgcgtt tggtgtcact ccaaaagcag gttatttgga
     4561 ggaagttcat acgaaagcag gtgtctgaca tggtgaaaaa agctttttgc atgaaagttt
     4621 acccagaaaa acaagaagaa tacaaaaaaa gacatgatga actttggcca gaaatgcgta
     4681 cagcacttcg tgaacacggg gtcatcgact attcgatcta tcttgatccg agtacaagtt
     4741 tgttatttgg ttacttagtg atcgaagatg aaaagttatg ggaaaaaatg gcaaatacat
     4801 ctatcaatca aaaatggtgg aagtacatgg aagatatcat ggagacaaat ccagatgcct
     4861 ctccagtcag ttgggaattg atgccagtct ttgaactgta gcaacaagca actgggcaac
     4921 cggttgcttg gggttctttc gtttttattg tgaaaaaaaa tacaaattag gaggaataga
     4981 aaatgagtaa tcgaatgatt ttaaatgaaa catcgtattt tggaaaagga gcgattgagt
     5041 cgatcgtccc tgaatttcag cagcgaggat tcggcaaagc agctgtcatt actgacaaag
     5101 gattgatcga gcacggtata gcaacaaaag taacagaact tctagataaa gcagcaatcg
     5161 cttatgcttt atacgacggg atcgtaccta atccaacgat ccaaaatgta aaagatggag
     5221 tggcttttgt taaagaggca gaggcagact gtttgatcgc aatcggtgga ggttcgccaa
     5281 tcgatacggc aaaagccatt gggattattt tgacgaatcc agagttttca gacgtcatta
     5341 gtttagaagg tgttgctgat acgaaaaatc catgtttacc tatattagct ataccaacga
     5401 cttctggaac agcagcagaa gtaacgatca actacgtcat cacagatgaa gtaaatcacc
     5461 gaaaatttgt ttgtgttgat cctcatgata tcccaatcgt cgcgttcatc gacagcgata
     5521 tgatgatggg aatgccgaaa aaattggctg catcaacagg tatggatgct atgacgcatg
     5581 ctattgaagg gtttatcaca aaaggcgctt gggaaatgac tgatatgctg catttaaaag
     5641 caatcgaaat catcggccat tctttggaag tttctgtaga tggcgatcaa aatgggcgag
     5701 aaaaaatggc attaggacag tatattgcgg gaatgggctt ctcaaatgtc ggtttaggac
     5761 tggtacacgg gatggctcac ccgttaagtg catggtacaa cattcctcat ggcgtagcgt
     5821 gtgcagcact tttgccaact gtcatgaaat ataacaaaga atatactgga gaaaagtacc
     5881 gtgagatcgc tcttgttcta gggattaaag gagcagctga aatgtcttta gaagatgtaa
     5941 gagaagcggc ttgtggtgag atcgatcggt tgagcaaagc ggttggaatc ccagaaacca
     6001 tttctgaatt aggtgtaaaa gaagcagata ttccagcaat tgcagaagat gctttgcgag
     6061 atgtatgcac accaggaaat ccaagagaaa cgacagtaga agaaatcatt gctttatatc
     6121 aaagtttgat gtaaaaacga agcggatctt caacattaaa ttgcctgtaa agagaaatgg
     6181 tagtttatat ctggatatct cttttaaatt gatggtattt ccgctataat ttttcttata
     6241 aagaaatagg agaatattat atggaaaata aaaagcctta tggaactgta ttgatcaaag
     6301 cctctaaaat actagactat ttagcggaaa accaagatgt ctctttgcag acgatcgcaa
     6361 agggtgtggg aatgacctct tctacagtct tgaaaatact agatactttg ttgatgatcg
     6421 gctacgtaaa taaaaattca gagaaaaact atcgtttagg tgcaaagctt attcgctacg
     6481 cgaataaaaa cattgaacaa atcgatttgg tagagttgac tttaccgttt ttagaacagc
     6541 tgcagcaaaa gatcgatgag actatccact taggggtatt ggacaacaat gagatcttgt
     6601 acgtgaacaa actagatcca aaacatcaaa cgatccgcat gtcttcaaaa atcggaatca
     6661 cccgaccatt atacaattct gcgatgggca aagccgtatt agcagaattc agtgaagagc
     6721 agtacactga ttatcttgaa aagcatccgt tgatccctta cacagaatat acgatcacca
     6781 atgcattacg gttgaaaaag gaaatccaaa cggtaaaaga aacccacatc gcatttgacg
     6841 atgaagaaat cgaacgtgat attttttgta taggagcctc aatcgtgaaa aacgaacaaa
     6901 tcattggtgc atttagtgta agtatgccga aatatcgttt aacagatgag acaaaagaac
     6961 aaataatcag tgccctcaag caaacaaaaa gcgaaatcga gcaaagaatt caaaaataat
     7021 gaaaaaagcg tttgcaattt ttcttttctg ccgtataata tgggtataga tttccaagtt
     7081 ataaaataaa atttctaaat aggaaatagg tggagaagat gaaaaaaatt gatattttat
     7141 ctcgattgaa aaacgccgga gtgattgcgg tggtacgcgg aaaaagcaaa gaagaagctt
     7201 tgaatgcttg ccacgcaatc atcaaaggtg gtctaactgg tattgagttg acttttacag
     7261 tccctcaagc agatcaagta atcaaagaac ttttgtcatt ctacaaagac caaccagaaa
     7321 tagtgattgg cgcaggcaca gtattagacg ctgtcacagc tcgcttggct atcttagctg
     7381 gcgcagagta tattgttagc ccttcctttg atcaagaaac ggcagagatg tgcaatctgt
     7441 atcaaattcc ttacttacca ggatgtatga ctattaccga gatcaaaaca gcgctgaaaa
     7501 gcggggtaga tatcgtcaaa ctatttcctg gaagcgctta tggaccaagt atcatttctg
     7561 ccttcaaggc gccgatgcct caagtcaata tcatgccaac tggcggtgtg agtctggaca
     7621 atatgaaaga gtggttcgat gctggtgtcg taacagtcgg agtaggtggg aaccttttag
     7681 cacccgcagc tacaggagat tttgacaaag taacggaagt cgcgcaacag tatgcagcaa
     7741 aaatgaaaga aatcaagagg tagctgctta tgggaaaagt agtgacactc ggagaaatca
     7801 tgttgcgatt gtccacagag cagggaaccc gtatcgattc aagtgaacga ttcgctgccc
     7861 attatggcgg aggggaagca aacgtagcaa tttctttagc taattacgga catgaagcga
     7921 tttttgctac aaaagtacct gataacgcat tgggaaaagc agtcaaaaag cacttgcaat
     7981 gttatggagt agatacgaaa caggtattat ctggtggatc aagactaggt acttattata
     8041 tggaaactgg tagtggcgaa cgcgccgcct cagtgatcta tgatcgtgcc ggatcaagtt
     8101 ttgctgaaat ggatcatttg gaatggtcgc tagaggagtt attccaagga atagatatat
     8161 ttcatgtttc aggaatcacc ccagctttgt ctaaaaaatg gcaaaaaatg acgaaacagg
     8221 tgatagaagc tgcaaaaaaa gctggctgcc tgatcagctt cgatatcaac tatcgtggaa
     8281 aattatggac acagcaagaa gccagcgaag taatccatca gcttttacct ttagtagata
     8341 tctgctcagc tggaaaaatg gatgctcttt atttgttagg tatcgagaaa gcgccagaag
     8401 cagaagaaca acctctaatt tattattatc aaaaaatgca ggaaaagttc ccgaatattc
     8461 aggtgtttta ttcgaccaag cgtaccgttc attcagcaag tgaaaatgaa ttgaagggga
     8521 cattgtggat gaaccatgaa tatgtggaat cccaagtcca tagaatcacc ccaatcgtgg
     8581 atcgagtagg tggcggagat gcttttgcag gtggcgtgct ccatgggatt ttatccaata
     8641 tgtctccgca agaaatcatt gattttgcta cggcagcttc tgccttgaag catacgatcc
     8701 atggggactg caaccaattt agtcaagaag aagtcgcaca atttttagca tgcggctctg
     8761 gaaaaatcat tcgatagaag gagtagaaga aaatgcaaga aatggaaaca cgttatacac
     8821 acagtccaga agatatccgt cattattcaa cggaacaatt gagaaaagaa tttttagtag
     8881 aaaaggtatt tgaaccaggg aaaatcagtt tgacttatac gcacaacgat cgaatgatct
     8941 ttggtggagt gacacctact gaaaaagaat tagaaatcac attagacaaa gaactaggtg
     9001 ttgattactt cttagagcgt cgtgaactag gtgtgatcaa tatcggtgga ccaggattca
     9061 tcgagatcga tgggcaaaaa gaagaaatga aaaaacaaga cggatactat atcggaaaag
     9121 aaacacgaca tgttgtattt tcttctcaag atgctgaaaa cccagctaaa ttctatatta
     9181 gttctgttcc agctcatcat aaatatccaa atgtaaaaat cagtatcgac caagtaaaac
     9241 caatggagac tggtgaaggc ctgacactga atgaacgtaa aatctaccaa tacatccatc
     9301 caaatgtatg cgaaagctgt caattacaaa tgggttatac gattttagaa ccaggaagcg
     9361 cttggaatac gatgccttgt catacacatg aacgtcgtat ggaagcttat gtctactttg
     9421 attatgccaa tgaagataca cgtgtgttcc acatgatggg caaaccagat gaaacaaaac
     9481 atttagttgt agacaatgaa caagccatta tttcgccaag ctggtcgatc cattcaggtg
     9541 taggtacaag caattactca ttcatctggg caatgtgcgg agaaaatatc acctatacag
     9601 acatggatat ggtgcaaatg gatcaattaa aataagcata agggagagaa aatgatggaa
     9661 tttagtatgg atatgtttcg tttagatgga aaagtagcat tagtcaccgg tgctgtctat
     9721 ggtattggat ttgagattgc acgctcttta gctacagcag gagcaaaaat cgtatttaat
     9781 aacttgaatc aagaatcagt tgatgaaggt atagcgcatt ataaagaagc aggcatcgat
     9841 gcaaaaggtt atgtctgcga tgtgaccgat gaagaagcgg ttcaagcgat ggttcaacag
     9901 atcaaagaag atgtaggttc agttgatatt ctagtcaaca atgcagggat catcaaacgg
     9961 acaccgatga tcgaaatgga tgcggcagac ttccgccaag taatcgacgt ggatttaaat
    10021 gccccattca tcgtttcaaa agcagttatt ccggatatga tcgaaaaagg cggcggtaaa
    10081 atcatcaata tctgctccat gatgagtgaa ttaggacgcg aaacggtcag tgcttatgct
    10141 gcggcaaaag gcggattaaa aatgctgacg aaaaacatcg cttctgaata cggtcaatac
    10201 aatatccaat gtaatggaat tggaccaggt tatattgcta caccgcaaac tgctccatta
    10261 cgcgaaacac aagaaaacgg tgaacgtcat ccattcgatc aatttatcgt tggacgtacg
    10321 ccagcagcac gctggggcga gccaatcgat ttggctggac catctgtctt cttggcttct
    10381 agcgcatctg atttcgttaa cggccatgtc ttgtatgtgg acggaggcat acttgcctat
    10441 atcggaaaac aaccttaaaa gagctttagt acactgttta gtggagaggt tgtgaaatca
    10501 gcggattgaa cgaataccga aggacagctg attaaacatc atttattcga aaaaagaggc
    10561 tgggacagaa gcatttaact ccaagaaata agaaggaatt cacgaaaatt gcttttcaaa
    10621 tttttgtgaa tttcagctta tttccgaagg agttgctttt acttccgccg tttgtacgtg
    10681 tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctcttttc tgtgcgtctt
    10741 ccatacttca ctaagtcgat ccctcttgct tcgcaccaat ccttcactgg tccggaaaag
    10801 atcagagatt gttggtgtag ggccgcggta ttcgttgcgc cgaccatagt ataaagcaga
    10861 cgaagctctt cttgccattg cttcatcagt ataatagttt cttctacgcc gtgattcatc
    10921 agatgcgtca gtacggttcc tgaagtaccc acggcatttg cgccgagaca aagggcttta
    10981 aatatgtcat aggcattgcg gatacctccg gaggctagga tttccataga agtgtctgct
    11041 tcatttgctt caagcaatga agagactgtc gattgccccc agtcagctaa ataactcagt
    11101 tcccgcttgg agcgacgggc gttttcgatc tgggtgaagc ttgttccgct tcgaccgctg
    11161 atatcaactg tatgaactcc taaagcagct agatcattga gcgtttctct tgtcatgcca
    11221 aaaccgactt cttttacgat gagggggacg tcaatggctg tttgagtttc ttgaataagt
    11281 actttccaat tggtaaaatc acggtctccc tcaggcataa ccaactcttg aggtgcattc
    11341 aaatgaatct gtaaggcatc tgcgtgaaac aatcggatag cttctttggc tctttcgaca
    11401 gaagtccctg caccgatgtt ggcaatgatt ttcccgtgag gatattcttg tcgcataatc
    11461 gtataagtat cggctaaaga agggactttc aaggcagcgc tgactgaacc agtggcaatc
    11521 atcaaatctg cttctcttgc gacgattgca agatcttggt tgattttttt tgttttttcg
    11581 cttccgccag tcatcgcatt gatataaaat gggctgctta gtgtaaaccc aactgcctgt
    11641 gtacttagat cgacatcaga aactgctatt tgcggcaaag ggttatggat gatccgtacg
    11701 aagtcaaatt cgtttttttg tttatcatga aaggctttag ctagtgaaac atgttcgtct
    11761 tttcgattca tcggttttcc tttctttgat cgctatagac atgtaacggc agtggagtga
    11821 tttctgcttt ttcccatgca ctcattaaag gaagaatgcc agatttctgg tcaacaatta
    11881 cgattccgca atcgccccca cctgcaccag aagattttgc ggctccttca tactgttcag
    11941 ctaaattaca caatttgttc aaagcaggcg tttcgatgac gaccccagtg attgcagata
    12001 aatcatgcag taattgtcgg ttttttcgaa tcatctgttg gatcaacgtt acattatttt
    12061 ctttaaaccc tttgatcatt tcgttgacac attctgtact attttttaag aaaagctgat
    12121 aagccaccat tttatcttct ctcgaacggt gaacttgatc gaccaaatca gaagtagagg
    12181 cagggctacc cgtccaacca atcaataaac gtaaatcttc aggagcaatc aatggctcaa
    12241 tggatagacc tggccaatcc agggcaagta actcactgat agaatgctga gtttcttgtt
    12301 cttggagcca aggatggtcg aaggttgaga aagcgatcca gccaccataa cagctagcag
    12361 cgatgtcgcc gcaggaacca ttatcttgaa ctgctaaatt ggctagtgct gcaatcttga
    12421 aaatctccaa ctgagataaa ttcaacgcgt aaaaaacatt caaggctttg accgttgcaa
    12481 cggtcacggc accgctagaa cccaatccat atttccggcc atttgaactg tctaactcac
    12541 ttgttacttt cagatcataa aaggataaaa gaatgttttt ttcttgtgca tacttttcag
    12601 tcaagcgaat agcagcaagg atataatgaa aaggattttc ccgaatatct aaaactaatt
    12661 ctccgttgcg tcttgtccaa cgtacaggca tcccactata ttgtgcagat tggatacttc
    12721 cgacttttcg tgcagattct acagtgactg tcacgaattg atcgactgca gcgataactg
    12781 ctggatggcc tgtttcaaca actgcatatt ctccggcaat atagagtttg cctggtgcag
    12841 atacttcaat cataaaaatc tccctttatt caataatcgc aattcctgga ccagcatgtg
    12901 cagtaattac ttgttctttg ctaaataatt tggtaaatgt ttcttgtacc tcagataaat
    12961 ggctgttttc cactaaaact ttgacattag ggccagcatc catcgtaaag tagcatggaa
    13021 tgccttgctt tctcaattgt cgcaccgcat ccatcgcttt taagctgtca ggagaccagt
    13081 aagtgaatgg aggctgggca gctaaagtcg ttccatgcat ttttagtccg tttctttcca
    13141 tcgtttctcc gagaagttgg aaatcttttg ttttgattgc ttgtttcaat tgatatagat
    13201 ccccttcaac ggaatctaac cagccttgat aaaaactgga tgtttcgact gtccgacgca
    13261 tcccatttct gctggacact tcttttttct ggtcgttgat caaaacgaaa accattgcta
    13321 aatcgtcttc gaaagaatcg gaagggactg gcttagcgta agaacttaag tcgtcatggc
    13381 ctttttccca ttcgacgaaa ccaccgaaaa tacttcggca agctgaacca gacccgcgtc
    13441 gagcaaatct tgaaagagag agatcatcta atccaagctt aagagcagta ttgcaagcgc
    13501 ctgctaaagc agctagcccg ctggcagagg aggctaatcc agctgcggta ggaacgaaat
    13561 tctgactgat tactgaagct tttagtgaga ggccggcttc tttacgaaaa aggtcaagaa
    13621 actgactgac tttttttgtt gctttttcgt cttgcaaggt gccatctaga taaaattcat
    13681 ctaccatata gctatcagaa aagatgactt ctgtctctgt ataaaatgca tccaatgtta
    13741 acgaaaggct gttgttcatt ggaaggatga gttcttcatt tttcttaccc caatatttga
    13801 ttagagcaat gttggtatat gcgcgtgctt tgcctttaaa cataggtatg tactcctaat
    13861 ccttgtatcc aagtttcagc agcacccgca tcttcaagtg cttgactgat ttcttgggct
    13921 tttgtttttg tttgtgctaa tgcaatcata cagccaccgc gtccaccgcc agttaatttg
    13981 gcacccaatg ccccggtatc ttttgctgtt tggatcaata gattaagaaa atcattgctg
    14041 attgtcagct tttccagaag tgattgtgat tcatccattg tctgtgccaa cgtttctggt
    14101 gaattttcaa tgattgcttg ttttgcttgc ttcgtcaagt atcctaattt ttggatcatt
    14161 tgtccagttt catgaggctg ggtttcaaag aggtgagcga catctttgac ggcttctctt
    14221 gtttgtcctt tgattcctgt atcagcgaca atcaaaaaag catcgatgtt caaagaaaag
    14281 tagtcgaaag gatgcccttt tgtaaaataa atcggctgca agctgctagt agcggctgca
    14341 tcgattccac taggattacc gtgggcgatt ttttccgaaa gctggacatt ttctaatagt
    14401 atttcacgag acaaaggaaa tgctaagtaa tcataaaaag cacgagtgac tgctgtggcg
    14461 actgcagcgc ttgatcccat tccacgttca gcaggaatcg tgctttcaat ggtcagttgc
    14521 aagttttttt ggatgtcatg ctgttttttt aagtgaatga ataatttttt gatatttttt
    14581 aatgcatgag gagcttcggc taaatttcct gaatagtagg aagaaaccag ttgatcgtcc
    14641 attgcatcca gctcttcaag gaatgcggtg acttttgttg catagaaagg aaaggcaatc
    14701 gctggttctc cataaacaac cgcgtgctcg cccatcaata tgatctttcc gcttgattct
    14761 ccttggccat agtttgccat aatctaactc cttctcacgt aaaagtgatt tttaagcaaa
    14821 aaataaagcg cccaaaacga gttgcgctgc ttcacgaatt tttttgtcca ctaagggtct
    14881 caaaaaacat tcctccttta ttttactaaa accatctcag aagtaaaact aagaatacgt
    14941 tttctaaaga aaaaacactt ttttcttaag aattttttga ctttttttct tctttgacta
    15001 gaaaaaaata caattgacac tactagatat tgggggtata tttaaaaagg ttggagttaa
    15061 acacaatatt tagtgatgaa gtgagctgga ggtaaatgag cgtgatcgtt ttagcaggta
    15121 caattggtgc cggaaaatca agtttaacag aaatgatggc tgaacacttt gatagtcagg
    15181 cattttatga atcgattgat gataatgaag tcttgccctt attttatgca aaccctgaac
    15241 agtatgcttt tttgctgcaa atttattttt tgaataaacg ttttgcaagc atcaaacaag
    15301 caatgaaaga tgacaataat gtattggacc gttcgattta tgaagattca ctgcttttcc
    15361 atctaaatgc agatctagga agagcaacag aaacagaagt tcgtgtgtat gatgaactgc
    15421 tggaaaacat gatggaagaa cttccctatg ctgcccataa aaaacatccg gatttattag
    15481 ttcatattcg tgtatctttt gatacgatgt tggagcgtat cgaaaaacgt ggtcgttcgt
    15541 atgaacaatt gtcctttgat ccaagtctat acgattatta caaagaattg aacagacgtt
    15601 acgatcaatg gtatgaagaa tacaaagaaa gtccaaaaat ccaaatcgat ggagaccgct
    15661 ataattttgt ggaagatcct caagcaaaag aagaagtctt gaaaatgatc gaagaaaaat
    15721 tagcagagat tcgccaaacc aaagtagcca tttgatttac agttgctcaa ataaagggta
    15781 aacctttatt tgagcgactt ttttctttat actggaaaca aatgtcagga ggatagagta
    15841 tgcgggaaaa acactatttt aatcaagacc gtaatttttg gatcaatgta cagcaaaacg
    15901 atgtgctagc tgttaaaaac ttaaaagaag aatatggcat tagtgacgag atgctgactt
    15961 attctctaga taaaaatgaa cgggcgcgcg tagagtacga ttcttttgat gaagcgttac
    16021 tgcttgtatc aaatgttccg caccagcaaa aagtcgataa tcattatgaa actagtccta
    16081 tcgcttttat tttaaaagaa gacggtttgt ttacatttac cacgccgaat accgagtacg
    16141 tgattcgact aattcgatcg cttttggaac gaatgccaga catgagtgta tacagtttat
    16201 tgtttcgtac attgtttttg atttctgatt ccttttttcc tttaatcgag gaagtgaata
    16261 gtgaacgtca gcggcttaat ctgaaattaa gagaaaaaac aacgaacaag aatctgctgc
    16321 aactttcaga tttagagatc ggtttggttt acttagttac aggaacaaaa caaaatgtag
    16381 tacttttaga acaaatcaag gcattggcta tttatcgcaa gttgtcagaa aaggaaaaag
    16441 aagagttgga cgatgcgctg atcgaagcac gacaagcagt tgaaatgacg aacttagctt
    16501 cgcagatttt agaccagctc tctggcactt ataataattt gctgaataac aacttgaatg
    16561 acacgatgaa atttttgacg gtctggtcgc tgatcttgac cgttccaact attgtgacag
    16621 gattcttcgg aatgaatctc cagttaccct ttacccattc cgttttcgga tggggaatcg
    16681 ccttgatcat cagtttggtc ttatctattt ggatgcttat tgcactgtgg cggcggattc
    16741 gttgatctag tctttgatct gcaaaccaag ggcttgggcg atcacaagtg cttgctcttg
    16801 gttgacgatt agtcccgcca aagaatcttg agataaagcg attgctgtga agaaattagt
    16861 cgtaaagtct aatcccttca gagaagttcg aaaccagttc gaaccggtca actgattgtc
    16921 agacaaaaaa aggtttttcc aagtgacctc ataaaattcg ctgtcttcta attgacaatg
    16981 atcgaaggag acagctttca agtttgtact actaaaggaa gccatcgaag caatacaatc
    17041 agtgaaagta cagtctcgaa gataactttc ggcaaaattc gtacctgtta atttacattg
    17101 atgaaacacg acttggtgga aactgccacc aatccactcc agattggaaa aatcacattt
    17161 ttcaaaaatg acattactgc attcaaaatg acgtaaaact gttttttgca tcgtaagttt
    17221 ttggatatga ctctctctta ataccatatt tttggcatcg agatagcttt ggtcaccccc
    17281 gttgatcaga agaccagaga agatctcctc atcttctaaa taaaactctc cctttgtaag
    17341 aagaggaaga gaaggtggaa taggttcgat ttttttcatg tgggttcacc tcgtttttct
    17401 ttagtatagc aaagaagcgg gaatcactgc tagaaagtct gctaatctaa tcattttcag
    17461 aaaaggaaga caaactgtat gaaaaacatt gacaaaaaga ttagtttttg ctatactatg
    17521 atggttgaga aataaaagca gaagcacccg cttctcgcct tagttgataa tgtcactggg
    17581 ctagatagtt ttcactggtt ctattaggtg aagtacgcgc gggttctgta agagaactcg
    17641 tttttttatt gccgtttatt cggtaagctt ttctttctct cataatattt ggaggtgaat
    17701 gaccatagca aaggatatga tggttaacga cggcattcgt gcacgagagt tacgattgat
    17761 cggttcagat ggtgagcaat taggagttaa gacaaaagca gaagcattga atattgcaga
    17821 acaagcaaac ttggatcttg tgttagttgc ccctggtgcg aaaccaccag ttgcgcgaat
    17881 catggactac ggtaaatacc gtttcgaaca acaaaagaaa gaccgcgaag cgcgtaaaaa
    17941 acaaaaagtg atcaatgtaa aagaagttcg cttaagtcct acgatcgact tgaatgactt
    18001 caatacaaaa cttcgtaatg cacgtaagtt cttggaaaag ggcgataaag tgaaagcttc
    18061 tatccgtttc aaaggccgtg ccattaccca taaagaaatt ggtcaaaaag tcttgaatcg
    18121 cttagctgaa gaaactgcgg atattgcaac agtggaacaa aaagcgaaaa tggacggacg
    18181 cagcatgttc ttgacgctgg caccgaaaaa cgacagtaag taatttgaat gactaactgg
    18241 ctgttggaca gccgatagtc ggataacgaa agattttgag gaggaaatat agtcatgcca
    18301 aaacaaaaaa cacaccgcgg atcagcaaaa cgtttcaaac gtactggtaa aggcggattg
    18361 aaacgcttcc gcgcgtttac aagtcaccgt ttccacggca aaacaaaaaa acaacgccgt
    18421 caattgcgta aagcaagcat ggtttctagt ggcgacttca aacgtattcg ccaacaacta
    18481 gcaaaaatga aataagaagt acaaataaca gaatctgaag atttattttt attaggagga
    18541 attatacatg gcacgtgtta aaggtggaac agtaactcgt aaacgtcgta aaagagtgct
    18601 aaaattagca aaaggttact acggttcaaa acacacttta tttaaaacag caaaagaaca
    18661 agtaatgaat tcatacaact acgcatatcg cgatcgtcgt caaaagaaac gcgacttccg
    18721 taaattgtgg attgctcgta tcaacgcagc agctcgtatg aatggcttga gctactcaaa
    18781 attaatgcac ggtttgaaag ttgctgaaat cgacatcaac cgtaaaatgt tagctgactt
    18841 ggctgttaac gatgcagcag catttactgc attggctgac caagcaaaag aagctttggc
    18901 taaataagct aaacaattat aaagaggaaa gaagataacg aaaagttatg ttctttcctc
    18961 tttttttatt tacaatcctc catcaaatga aacaggacaa taacgtataa gtctaaaata
    19021 gcataattaa ggttttttaa ttgaaaactg aacatttatg gtcacccttt aatgaaagcg
    19081 ctaactacat aagttagaat gagtttgact gatatattag tttgcttatc agaaaaacaa
    19141 aatggacgga ggaataacat gagtgatatt attattggta ttttgttagt tatctcattc
    19201 tttttcatgg tctggtactg tgtaaaaggc tacaacttga tggtcggctt tgccatcatg
    19261 gctactgtat ggatgggatt agcgctagtc ggaaacacgt tttcaccaaa cccggcgatg
    19321 gaagggcaag gagtaattga tgtgttgacg catatctata cgacggggcc agctgagtac
    19381 gccaaatcga tcttggtaaa tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat
    19441 agcggaatcg cagctaccct gattcgaaag gtagttgaac ttggagggga caagccaaga
    19501 atcacgatga gcttactatg tattgtaacc gctgttatat tcatgtcgat gacaggaatc
    19561 ggaccagtga tttctattgc agttattgtg ttgccaatct tgatgtcatt aggtatttca
    19621 gtaccggttg cactgttctc atttatgggc tcgattatgg ctggaatctt tgctaatatc
    19681 gtcaatttca aacaatatca aacgatttat gctggtttca atccagcggc tgaaagctat
    19741 acttataatg actatttcca aattggtatg attgggatga ttgtaagctt agttgtggta
    19801 ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct atgcaatggc agcaaacgta
    19861 ccagctgaag gtggcgacgc accaatgatc tcatggttag ccgtattatt accagtttta
    19921 ggggtcgttc ttttagatct tccaatcatc ttaggtttta ttctagcagg tatctgggca
    19981 ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa tctgtcgtca gtttgcgaaa
    20041 ttatttacag atggcgcagt agatgttgca ccaatggttg gtttcttgat gaccttagcg
    20101 atgttcaata attcagctgc gtatgcttct ccttattttt cagctatttt tggagattgg
    20161 ataccgcaat caccattagt cttggcaatc gtatttgcta tattgacacc actaggattt
    20221 ttccgcggtc caatgaatct agtcggttca ggctcagcta ttttagctgt cgtattagct
    20281 gttaatccaa cgatgtcgcc agcctttttg ttcccgttat ttgcaatcac aacgattgct
    20341 cctcagcatt tagatattac acaatcttgg gttgcttggg gattaggtta tacaaaagta
    20401 acttctcgag aatatatgaa gaaatccatc ccaactggtt ggatcatcgg agcgatctta
    20461 tgcctgatca cgttcttatt gtacgggaac gcctagtaaa caaaacgtcg ataaaaacga
    20521 ttagatgaat ggaggaaaag aaaatgaaac gttttgtgcg aatgggaatt gatgttggcg
    20581 gtacacatac gaaagcagtc gcaatcgata atgcgacgca cgaaatcatc ggaaaatctt
    20641 cagtgaaaac aacccacgat gatgtccgtg gagtagccgc aggtgtcgtt cagtcttttc
    20701 aaaattgttt gcgggaaaat aatatcagtc cagaagatgt tgtgtttgtt gcacattcta
    20761 ccacacaagc gactaatgca ctgattgaag gagacgtagc aaaagtcggc gtaatcggga
    20821 tggcaaaagg tggattagaa ggttttttag caaaacgaca aactagattg aacgatatcg
    20881 atttagggaa taagaaaaaa atagagattg tcaatgcatt cttacctgtc aaacacttaa
    20941 atgtagaccg tgtttcagaa acgatttctt ctttagaaag agaaagagcg gaggtcttgg
    21001 tttcttctat ggcatttgga gtagataatg gggaaccaga acgagttgtt tatgaagcag
    21061 catcggtaaa atcgatccct acaacgatgg cttctgacat cacaaaatta tatggattga
    21121 caagaagaac tcggacagca gcaatcaatg catctatttt accaaaaatg ctagatacgg
    21181 caacctctac agaagattcc gtacgagaag caggggtcaa tgtttctttg atgattatgc
    21241 gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa acgacctgtt ttaaccatgc
    21301 tttccggtcc agcagcatca gtcatgggct ctttgatgta cttgagagcg tctaatggtg
    21361 tttattttga agtaggcgga acaacaacca atatcggggt cattaaaaat ggacgtccag
    21421 caatcgatta ttcaattgta ggtggccacc caacatatat ctcttcatta gatgttcggg
    21481 ttctaggtgt ggcaggcggt tcgatggtac gggctaatca gtcaggaatc atcgatgtcg
    21541 gtccacgttc tgcacatatt gccggtctag attatgcagt atttacggag acagaaaaaa
    21601 tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg agaccctgct gattacgtca
    21661 aggtagtgat ggaagacgga gaagaagtga ctattacaaa tacatgtgca gccaatgtcc
    21721 ttggattagt gcaagaagaa catttttctt atggaaatgt cccatctgca cgaaaagcca
    21781 tccaagcatt agctgattat tgtcatacga cggttgaaga catcgcagaa cagatcatgg
    21841 aaaaatccta tgctaagatt gaaccggtga ttttagaatt ggcagacaag tatcacttgg
    21901 agaaagatca gatttctctt gtaggtgtag ggggcggcgc tgcctcattg attacttact
    21961 ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa cgccgaagtg atttcttcta
    22021 ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg tatcatccct tcgccaagta
    22081 aagaagatat acgatcgttg aaaaatgaag cgatgaataa agcgattgaa tcaggcgcta
    22141 caccagaatc gatcgaagtc cacgtagaaa tcgatccaca gacttcaaaa gtaacggcta
    22201 tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac caaggagatc acaacagaag
    22261 aagcactaga gcttgctgca gaggatatgc gattaaataa aaatgaagtt tgcttattgg
    22321 aaaatacacc tttcttttat gtatgcggcg aacagaatcg ttcaaaaaat gctggaagtc
    22381 ttcggatcat cgatcaaaaa ggatttatca aggtccaacg tggacatgct tcatgtatga
    22441 aaacaactgc agccaattat atgacagctg tcgaacaact ttgggaagat atggcagttt
    22501 atcaaactga attgattgcc cgaccagaat tttatctctg cttaggagca agagtctctg
    22561 attttacagc gacggatctg gaacaactac agcttttgat ggatctggaa gtatctacta
    22621 tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat caaacaaaca taagttagaa
    22681 gagttactaa acgaattaca acagatcccc gaagaaattt ggggattcta ccagtttcgg
    22741 cgagatttat tttggaaaaa gattcctttg tccaagcaga aaattcttat acaacaatca
    22801 atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa agtacccttt tgccgatgta
    22861 ggagaaattt gtgaacaaat ggctgttcca atcgtttcct gtgaatcaga gcaaataaac
    22921 gaacgaatca cttttgcaac ttatgcagaa gatgaaggga ttcgattgat gacggagccg
    22981 ttagaaaaat taaaatgttc aggactagct tcgatctcta aagaaacagc gcaagctttg
    23041 attattggac atgaactatt tcaccacatc gaagcttctg ttaagggaat ttatacgcaa
    23101 agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac atcaatcaaa cattcgtgca
    23161 ttgagcgaga tagcagcgat gagtttttca aaagagatga atcaaagcga tttttcgcct
    23221 tatgtattag aggcagtctt actttggcca tataatgaaa ttcgtagcca agggatctta
    23281 gaagaaataa aaaaaataga aaaaagatgt acggagtatg atttagatta caaataaaga
    23341 gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa taaacgatgt tctcctgtca
    23401 actcctcggt cacaagtcaa aatattcaac aaacatgaga agctgttttt tgaaattgtt
    23461 ccttgtgact cggagatgaa cggcaggctc acgacctctt ggaaaacggt gttcaaaagc
    23521 ttgcatctgc ggtaataagt ccagccaagt taaaaacata agaagctatt tttacttggc
    23581 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctcaataaac ggtgtgcaat
    23641 cagctgaccc tcggtattag ctcaaaaaaa acggcaaaat atgaggaact attttgccgt
    23701 ttctttctct aatactcagg tcaaaacgct gattccaaaa cctcttattt gatgggtctg
    23761 tcctgaatat cttcaaaata atcggggtaa ttatgaatca cgtattcttg ctcctcgata
    23821 atcatatgta aatgcatcgt gcaaagaaat ccgacttcat caaagttatg atcaatcatt
    23881 gattgaagca ttgtctgatg ttcatctaat actcgacccc agtctaattc tgaaatcgtc
    23941 aaacgtaacc atctgaaacg gttcaagtgc gtactataac tttctagcca atcccaaatt
    24001 tctcgttttc cagcaatttc ataacaagtt cggtgaaaat cagtatccaa atcaaaaaaa
    24061 gcttttttat tttgagcgat tactgcctga tcggtttttc caacttttcc cgcgtataac
    24121 gggctaatga agcagatcgg atatcgattt ttgaaacata agtgccggac tgtggaatgg
    24181 tttttacaag ctcttgattg cgcagttgga tcagtgcttc tcgaatcggt gtacgcccga
    24241 tagctagttg ttcttctaac actttatcgg agattttact tccaggtgct aactcggaat
    24301 aaatgatttg tttacgtata gattcataag cttgctgttg caaattattg ataaccattt
    24361 aaccttcctt tctatgcaga tataatctat tataacataa atcgcttaca aatcgtgtgg
    24421 gcataaaaac acagtctgca aaaaaagccc tgataggcat gcctattaga gcctcgaaaa
    24481 gaaaaatatt gaatcaatct ggtcgtacct cttgtatcgg tttgacacga ccgccgattc
    24541 ttttagcaaa agcttcggat tcttttttgt ctttgaaaat gagcagattg tctaagtttt
    24601 tttcccgagt gcgacagcca gttttatcga tgtaaccgtg cattacttta acgacataca
    24661 tggggatcac ctgctttttt cttttcatta tacgaactac tcgacacaat acaagaaaaa
    24721 tgcttagacg gtctttatta agaatttaca tcatccaaag aagcgtacgc ttggaataac
    24781 tgatctaact ccggattgac tttgctcagc ttcactaatc gttgcgaatc agaaagtaaa
    24841 aaacagtgtt tactactgcc agtagttttt aattttttgt cttcagttcc cacaatttcg
    24901 tagctaaaat ccaaccgtgt ccccgtatac ttttggatag ttggatggat gctcactttt
    24961 tctccgtaat ggatcatttc tttatagctg caatttactt ccagaacggg gatgatgatc
    25021 ccagattctt cgattttttt atagggaaac cctagatgta ccaataaggc tacccgagcc
    25081 tcttcaaacc agcggatata attgctgtga tgaataatcc ccatttgatc tgtttcataa
    25141 taaaaaggtt ctcgcacata acctgtaaat ttctccatga ctcatgcctc gttttccatt
    25201 gattttttac aaaaataatt catccatttc ggaagtgctt cgctgatttg tgtcggcaaa
    25261 acaatatatt gttcttttgc cagctggtct ccaatgaaac tatgaagata aatagctgct
    25321 gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc ctgtgatcat cccagctaaa
    25381 gtgtcaccca tgccgcccgt tgccatagca gcagaaccta aaggattggt gtagaagtgc
    25441 tggtgaccat aaatttttgt ctcatgactt ttcaaaacga gcacagcacc gatttcctgt
    25501 tgcttctttt tgttttcttt atcccgttgt tcttgtatcg gtagagaact caaccgttgc
    25561 cactccattt gatgaggtgt aagtacagtt tgttcgggat agggtagcaa aagatgatta
    25621 gcagcaagta aacttaaagc agagccatca atcaccagcc attgttcttt tgattggttt
    25681 tctagtactt gtttgaaacg ttccaaactc ttagaagaag tacccaatcc gggaccaatc
    25741 aagagtacat ctgcttgctt tattacggaa agaaactggt cagtatccgt ccaaactaca
    25801 atcattgctt ctggcagatg actgtgaaga gcactccgat ttttttcatc agttactaca
    25861 gtcgtgagac ccgctccgct gtgtacggca gctaaagcac tcataatgat tgctccgcca
    25921 tattgttcat tgccgccaat caaaacgata cgtccaaatg ttcctttatg tgaaattttt
    25981 ggacgaggtg tgatgactct tttcactagt tgttcgttta tcagcatcta aaaacctcct
    26041 tttactcatt atagcgtata ttttttttag agaaaaacag ataagaaaag gaattgtcag
    26101 attttttgaa ctattttttt ctgtaatttg tggtatcatg aacacgaaga cttaaataag
    26161 tggaggatta actttatgac aattgattgg caaaaagaag tagaagcacg taaagaagac
    26221 ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg agcgcgatga ctcaaaagta
    26281 acaccagatg caccatttgg acctggacct cgcgacgcat tgaaacatat gttagcttat
    26341 ggcgaacgtg atggatttaa agtaaaaaat gtcgataact atgcagggca tattgatctt
    26401 ggcgaaggag acgaaacgct agggatattt ggacacatgg atgtcgttcc tgcaggagat
    26461 ggctgggaca cagatcctta tgaaccagta atcaaggatg gaaagatctt tgcacgcgga
    26521 tcaagcgatg acaaaggtcc aagtatggca gcttactatg cgatgaagat cattaaagaa
    26581 cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg gcagcgatga agaaagcggc
    26641 tggggcgata tggcttatta tttcgaacat gaagaagaac cagattttgg tttctcacca
    26701 gatgctgaat tcccaatcat caatggtgaa aaaggaaatg tttctttagc tttacgcttc
    26761 aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg tttccggact tcgcgaaaat
    26821 atggtgccag gaacagcaac tgcagcactt caagtaccaa gtgcagatgc agctatcgcg
    26881 atggaagaag cattttacca attcatcgaa gcaaatccag taagcggaac gattgaagcg
    26941 gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg cacatggtgc aagcccacaa
    27001 tctggtatca atgcaggttc tttcttggca ttgttcttag ataactatga attcctagga
    27061 tctgctaaac aatttatcca tgtagcagct gcttatgtcc atgaagattt ttatggagaa
    27121 aaactaggag tcgcttatga agatgaaaaa atgggcaaat tgacaatgaa tgctggacta
    27181 tttgcttttg aagaaaatgg tacagaggaa gcgaacttca tcaatatgaa tttccgtttt
    27241 ccaaaaggtg taacagtaga cggattgcaa agtgacatcg aacaaacagt ggggcaagaa
    27301 ggcgcaacag ttactcgagg tgctcgcgta atggaaccac attacgtacc aatggatgat
    27361 ccattggtgg ctacattatt gcaagtctat gaagaccaca ctggagaaaa aggctacgaa
    27421 caaatcatcg gtggcggaac ttacggacgt ttgttgaaac gtggcgttgc ttatggagcg
    27481 atgttcccag gatatactga tacaatgcat caagcaaacg aatttatgag tcttgatgac
    27541 ttgttcagag caacagcaat ctatgcagat gccatctatc gtttggcgaa ataattaatt
    27601 aaaaagagga tcaagaattg caaattattt ttgcagtctt gatccttttt ttactttcta
    27661 ctgtggtatt catgataaac atgatataga ttcaattcat attctttctt agcgttcgtg
    27721 acgactgtat ccaaaatcaa ccctgttact aatgaaagaa gtgctaaagt catcaatcca
    27781 gtacttaaaa tcgcagaagg gatgcgtgta ataaagcttg tcagcatata ttcgcgaatc
    27841 acaggaatcc ccgtgaccaa tccaaataaa aagaagaaga tggtccaaat cccaaagaaa
    27901 agcaaaggct tataatcttt aaacatttta ataatcatca tgatcacttt gaatccgtca
    27961 gagaatgtat tcaattttga ttcgctgcct tcaggacgat cacgataatc gattgggatt
    28021 tcaacgaact tgaatttttt atctaaagca tggatcgtca attccgtttc gatttgaaaa
    28081 ccagaactca taataggaaa acttttgaca aagatacgat tgaatcctcg atagccggtc
    28141 atgacatcaa gaatttttgt tttatacatt ctagtaattg tatttctaac aaggttattt
    28201 ccaaaatcgt ggaaaggtct cttattctca tcaaagtagg ttccatttga taggcgatca
    28261 ccaatgacca tatctgcttc accagaacgt aatttctcga gcaaaccatg tacagcttct
    28321 gcaggataag tatcatcgcc atctaccatt aaataatagt cggcatcgat atcaaagaac
    28381 atttggcgga tcacatttcc ttttccttga cgaggttctt tttttacgat tgctccacct
    28441 tgaacggcca attcgtatgt tcgatcggtc gaattattat catagacata aatatctgct
    28501 tctggtaatt ctcgtttaaa gtctgcaatg actgttgaaa ttgttgcttc ctcattgtaa
    28561 caaggaatca gaacagcaat tttttcttgt tgttccattt ttcactctcc ttaactatag
    28621 aaaacaaaag ctaaattaaa aatacctcat aatcaaagga aatgctagtt tttcccgcta
    28681 aatatcattt atttaaaaag aaaatactgg aatatatgtt tggaaagctc tatctaaaaa
    28741 accacccact acaaaagaag gacatgatac ctctcttttg aatagtgggt gcgtattcgt
    28801 tctctcactt ttttgacgta ttaatctgtt gcaccttttt ttcttgaaat ttctaatacg
    28861 gttcctgtat gagcatctgc taaaaattca taataaacag tttcgccatc ttctattcga
    28921 gaaagacctc cccgataagc actcgaccga acggcgaatt tcctaatagg ttccttttca
    28981 aaagagatcc aagaaccttc gattgcacct tcttttagaa aagatgactt gatattttca
    29041 agtacttggt ctgctgacaa tacttttttg cgatgatacc aaatcgttga agctaccccg
    29101 ctcaataatc ctagaccgat tccaactgct aacccgcctt taaaatagct tagttcgctt
    29161 tcttcataca tgtggacata cctccttctt ctcacttgtt actccttgtg cttattttac
    29221 cacactctca tattttcttt gaaataaata accgctacca gaaaaaataa gctataataa
    29281 atacgatcgg tctgcagaaa aaatcatatt ttatccgcag atcaatataa gtctgagagg
    29341 agtttagtct gaaatttaga cgaatagcat atggaagaaa aaacatttca acgaatcaaa
    29401 gagttaacag aattacaagg aacaagcggt tttgaacaag atgttcgcgc ttatatggaa
    29461 agtcatatgg aaccgctggt agatgagtta cagttagatg gtttgggcgg catttttggt
    29521 ctgcgccatc atgaagaagc ggatgcaccc cgtgtaatgg tcgcagcaca tatggatgaa
    29581 gtcggattca tgctgacaca aatccaagaa aacgggcttt ttaaagtcgt acctctaggc
    29641 ggctggaatc cttacgttgt ttctgctcag cgttttacac ttaaaacctc tactggtaag
    29701 aattatccat gtatttcttc ttccgttccc ccacatttat tgcgaggaac aagcggacaa
    29761 aaatcagtag aagtaaccga tattttgttc gatgctgggt ttgaatcaag agaagaggca
    29821 atgagttacg gtgtccttcc aggagacaca atcgttcctt acgctgaaac gatcaaaaca
    29881 gccaacggaa agaatattat cagtaaatca tgggacaacc gctatggctg tacaatggta
    29941 ttagaagctt tagaagcgtt gcaaaatgaa acattaggtc atacattgat tgcaggcgcg
    30001 aatgtccagg aagaagtagg cttgcgtggt tctaaagctt ctgtcaataa attcaagcca
    30061 gatctgttct ttgcagtaga ctgctctgca gcagatgata cagtgacgaa aaatggcaca
    30121 ttcggacatt taggagaagg tacattgatg cgtatccaag atccaggttt gatcatgctt
    30181 cctcgtttgc gcgagtactt gctggatatc gctgaaacaa acaatatccc ttatcaatac
    30241 tttgtttcaa aaggcggtac agatgcaggt gctgctcata cacaaaacga aggaatccca
    30301 agtacagtga ttggcgtagt gggacgttat atccatacgc atcaaacgat gttcagtatt
    30361 cgcgattttg aagctgcacg agaaatgttg attcaaacac tgaaaggttt ggataaatca
    30421 acagtgaata cgatcgtata cggaaaataa tgcgatagtg aaactataaa atctggcaaa
    30481 agattgcttt ttgccagatt ttttttataa aaagaggatc aatgtttgtt agttcgaaag
    30541 aaaggtgtat aatttaattg aaaaatacat aaacagaaag tagaggacaa agaatgatta
    30601 tcccaaatag tttagaagaa ttagcaggct acgtagaaac tggaaagagt gtatttttct
    30661 ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc gcaaatgcct gaaattgaaa
    30721 atgacttttc tgattggcaa tttattgaag tagaccgtga caagtacata gatgtagccg
    30781 ctgaatggaa catttttggt atcccaagtt ttgtcgtgat ccaagacgga aaagaattag
    30841 gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat cgaatcgttc ctagaaagtg
    30901 taacaaaagg ctgacatctg ttaggagggg tcaagatgaa tcaagaatat aagaatattt
    30961 tagtaggtat cgacggtagt gaacaagcac ttgctgcttt caaaaaagcg gtagaagtgg
    31021 ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat cgatcagcaa ttttataact
    31081 ttatgggcta tgcacctatt gatcaaaatc tagtcgatca gcaaactgaa acagccaagg
    31141 aaatgatcga agaatgtaaa gaatatggga aatctgttga ttacacaaat atagaaggaa
    31201 ttattgcata tggttctgtt cgtgaatcca tggcagtcaa attgccaaaa aaatatgaga
    31261 tcgatctaat catggttggg cagtcaggtc taaatgcagt cgaacgcttt atgacaggaa
    31321 gtgtagccag ctacgtgata cgtcattcac cttgtgatgt cttgatcgtt tctgacgaag
    31381 aagaacagcc aagtaattaa gcaagtggaa cttgtacgac ctttttaatc aattaactaa
    31441 aacagtaaag ggactgtgac aaaagtcttg tcacagtccc ttgttacgaa taaatggtgt
    31501 tccagaagag gcttcttcgg aaataaagca tgaaaaaaat ttcatgcagt gaatgcaatt
    31561 ttttgttaag ataaatctca gcaaaggata atggaggaaa gaacatgatt tttgcttata
    31621 acaaagcaca cgtcggagac accttgatgg ttattatagc cgacgataaa ggaacagaaa
    31681 acactgttga acgcaaatgg aatgttgctc ggatcaaaga tgaacaaggt caagttgttg
    31741 cttggaactt tttccatatc tccgatcatc taacaatcga agggaacggc caagtaagct
    31801 taacagatga ggaattagcg aaattgaatc gattgatcaa agaagcagga ttcgaagaac
    31861 agcttgaagc tgatcatgaa ccgaagatcg ttgtcgggta cgtaaaaaca tgcgtcccac
    31921 atcctgattc tgaccattta tcgattactg agacagaagt agacaatggt acagtattgc
    31981 aaatcgtttg cggtgcaccc aatatcgaag ctggacaaaa agttgtcgtt gcaaaaccag
    32041 gagctatgat gccagatgga atgatgatct ggccaggaac tttgcgaggg gtagatagct
    32101 atggaatgat ctgttccgct cgtgaattgc atttgccaaa tgctccagca aaaaaaggaa
    32161 tcttagtatt gcctgaggat gccgtagtcg gagaagcatt tccaaaataa agaggttgtg
    32221 acaagtcttt gtcacaacct cttttttatt aattttcact attttgattt tgttttaatg
    32281 ccgattgatc gatcgtcaat tcaacagtag ttgttttttc tttcgagcct tcatagtaag
    32341 tgattttgac agaatctccc acttttttct tatacaaagc agattgaagt tctacaccag
    32401 aagaaacttc tttgtcatcg atcttagtaa tgacgtcgta ttgtttcaat ccagcttttt
    32461 cagccggtgt agctgtttgg acatttgtca cgatgacacc gttagttaca gatgaaggaa
    32521 tcttcaagat ttgttcttgt tgctgagtag aaacggcaga taagtcgacc attgtgatac
    32581 ctagagctgg acgtgtcact tttccgtctt tttccagttg attgatgata ttcactacat
    32641 cgttacttgg aatagcaaat cccattcctt ctacgcttac attagaagta gattcagaag
    32701 tacttgcgat cttacttgag ttgataccga taacttgtcc ttcgatatta actaaaggac
    32761 caccagaatt tccagggttg attgctgcat ctgtttggat cgcattgata ttgactgttt
    32821 cgttcgattc attcgtactt gtcacttgtc ggttcaagga agaaatgatt cctgaagtga
    32881 cagaatttgc atattcagat cctagtgggg aaccaatcgc aatcgcaggc tcaccaacct
    32941 tcagagcact tgagtcacca aaagaggcaa ctgtttcgac tttgtctgaa ttgatcttca
    33001 acaccgctaa atctgaataa gcatctgttc caactaattc agcttttacc ttcgtaccgt
    33061 ctttcatcaa gacttcaaga ccttgctggc catctactac gtgattattc gtgactacat
    33121 aagcagtatt cccgtctttt ttgtagatca cgccgcttcc ttcactataa gcttccaaat
    33181 tactatcatc tgagctgcta ctttcttctt gctgcccgaa tagtccgcca aaaccgcttg
    33241 attggttttg actttgtaag ttgataacag aaacgacagc gtcttgaact ttatctaccg
    33301 ctttggtgat atcagaatct acattgactt tgacattttc cacaactgtt tcaccggcag
    33361 aattctgatt gccgcttgta gcagtagaac tattgttgcc agtgcccatc gctgcataaa
    33421 aaatgccagc tgtcagtagt cctccaacga tccctccaac aagccccaga cctaacttac
    33481 gccagaggct attattttta ctttttttca tctttggtgt cacatttttt ctatccatgt
    33541 gttcctcctc caattacatt catttatcta ctgttagtat agtcttattt gttgacttta
    33601 gtctactttg agactgtgaa ctttttatga aaaactttag gaaaaatcat tgttttcaga
    33661 tgaaaatatt tgattttctt tttccacaga tactgtgtaa aactacgcgg ataggattgg
    33721 aaaaataagg gataaaacat atccacagcc tgtgtataaa gtggataaat tcttataaaa
    33781 cgtatgtttg cctaatgaat gtgatgaaat aaggtaaaat aacactgttt tctgtggata
    33841 aacctgtgga tgttgtgcat aactgttggg gaaatctttt taacaaaaat agccaaaata
    33901 aacatataga cgaaaaaatt gattataata aacaaagtga gcatgaagac caaaggagag
    33961 aaaacatgaa tatcaaaatt atctcagtag gaaaattaaa agaaaaatac ctgatccaag
    34021 gtatcaatga gtatgtcaaa cggctgaatg cctatgcaaa aatcgaattg atcgaagttc
    34081 ctgacgaaaa agccccggag aatttaagcg aagcgcaaat gagacaagta aaagaaaaag
    34141 aaggggaacg gatcttagcg aaaatcaaag aacaagaata cgtctatgcc ttagcaattg
    34201 aagggaaaaa tcctacaagt gaatcttttg ctaaacaaat cgaccaatta ggtatccaag
    34261 gaaaaagcca ccttgttttt gttatcggcg gctctttagg actcagcgaa gccgtcatga
    34321 aaagaagcaa tgctcagatc tcttttggaa aaatgacgta tccacatcaa ttgatgcgtc
    34381 tgatccttgt cgagcaaatc taccgtgcct tccggatcaa tgcgggggct ccgtatcata
    34441 aataatgata tttaagattg aatgctcctg aacactaagt tagcttttca ttgaaattta
    34501 tatataaaaa agttaacttg aaaatcgttg agaatttcaa aaaaataata ataact
//
LOCUS       DAZUMT010000018        32622 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000018 DAZUMT010000000
VERSION     DAZUMT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.018"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..718
                     /locus_tag="ACG8FM_000794"
     CDS             149..718
                     /locus_tag="ACG8FM_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5093479.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(896..1336)
                     /locus_tag="ACG8FM_000795"
     CDS             complement(896..1336)
                     /locus_tag="ACG8FM_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS5093480.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1494..2258
                     /gene="map"
                     /locus_tag="ACG8FM_000796"
     CDS             1494..2258
                     /gene="map"
                     /locus_tag="ACG8FM_000796"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS5093481.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2290..3213
                     /locus_tag="ACG8FM_000797"
     CDS             2290..3213
                     /locus_tag="ACG8FM_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS5093482.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(3438..3677)
                     /locus_tag="ACG8FM_000798"
     CDS             complement(3438..3677)
                     /locus_tag="ACG8FM_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093483.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(3682..4101)
                     /locus_tag="ACG8FM_000799"
     CDS             complement(3682..4101)
                     /locus_tag="ACG8FM_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5093484.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(4328..5566)
                     /gene="mef(H)"
                     /locus_tag="ACG8FM_000800"
                     /gene_synonym="mefH"
     CDS             complement(4328..5566)
                     /gene="mef(H)"
                     /locus_tag="ACG8FM_000800"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS5093485.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(5673..6308)
                     /locus_tag="ACG8FM_000801"
     CDS             complement(5673..6308)
                     /locus_tag="ACG8FM_000801"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5093486.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(6422..6514)
                     /locus_tag="ACG8FM_000802"
                     /pseudo
     CDS             complement(6422..6514)
                     /locus_tag="ACG8FM_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(6650..7285)
                     /locus_tag="ACG8FM_000803"
     CDS             complement(6650..7285)
                     /locus_tag="ACG8FM_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5093487.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            7797..8939
                     /locus_tag="ACG8FM_000804"
     CDS             7797..8939
                     /locus_tag="ACG8FM_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5093488.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            8932..9138
                     /locus_tag="ACG8FM_000805"
     CDS             8932..9138
                     /locus_tag="ACG8FM_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS5093489.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            9217..10410
                     /locus_tag="ACG8FM_000806"
     CDS             9217..10410
                     /locus_tag="ACG8FM_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5093490.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            10615..11754
                     /locus_tag="ACG8FM_000807"
     CDS             10615..11754
                     /locus_tag="ACG8FM_000807"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS5093491.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            11747..12547
                     /locus_tag="ACG8FM_000808"
     CDS             11747..12547
                     /locus_tag="ACG8FM_000808"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5093492.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            12547..13374
                     /locus_tag="ACG8FM_000809"
     CDS             12547..13374
                     /locus_tag="ACG8FM_000809"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5093493.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            13379..14086
                     /locus_tag="ACG8FM_000810"
     CDS             13379..14086
                     /locus_tag="ACG8FM_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5093494.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            14186..15052
                     /gene="rfbA"
                     /locus_tag="ACG8FM_000811"
     CDS             14186..15052
                     /gene="rfbA"
                     /locus_tag="ACG8FM_000811"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS5093495.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            15066..15638
                     /gene="rfbC"
                     /locus_tag="ACG8FM_000812"
     CDS             15066..15638
                     /gene="rfbC"
                     /locus_tag="ACG8FM_000812"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS5093496.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            15660..16688
                     /gene="rfbB"
                     /locus_tag="ACG8FM_000813"
     CDS             15660..16688
                     /gene="rfbB"
                     /locus_tag="ACG8FM_000813"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS5093497.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            16786..17637
                     /gene="rfbD"
                     /locus_tag="ACG8FM_000814"
     CDS             16786..17637
                     /gene="rfbD"
                     /locus_tag="ACG8FM_000814"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS5093498.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            17671..19704
                     /locus_tag="ACG8FM_000815"
     CDS             17671..19704
                     /locus_tag="ACG8FM_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093499.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            19748..21028
                     /locus_tag="ACG8FM_000816"
     CDS             19748..21028
                     /locus_tag="ACG8FM_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS5093500.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            21238..22044
                     /locus_tag="ACG8FM_000817"
     CDS             21238..22044
                     /locus_tag="ACG8FM_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093501.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            22056..23276
                     /locus_tag="ACG8FM_000818"
     CDS             22056..23276
                     /locus_tag="ACG8FM_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093502.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            23266..24852
                     /locus_tag="ACG8FM_000819"
     CDS             23266..24852
                     /locus_tag="ACG8FM_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5093503.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            24891..27029
                     /locus_tag="ACG8FM_000820"
     CDS             24891..27029
                     /locus_tag="ACG8FM_000820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5093504.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(27397..28380)
                     /locus_tag="ACG8FM_000821"
     CDS             complement(27397..28380)
                     /locus_tag="ACG8FM_000821"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5093505.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            28758..30149
                     /locus_tag="ACG8FM_000822"
     CDS             28758..30149
                     /locus_tag="ACG8FM_000822"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5093506.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            30165..31205
                     /locus_tag="ACG8FM_000823"
     CDS             30165..31205
                     /locus_tag="ACG8FM_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5093507.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            31224..32309
                     /locus_tag="ACG8FM_000824"
     CDS             31224..32309
                     /locus_tag="ACG8FM_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS5093508.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            32342..>32622
                     /locus_tag="ACG8FM_000825"
     CDS             32342..>32622
                     /locus_tag="ACG8FM_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFS5093509.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLS"
BASE COUNT        10657 a         5523 c         6440 g        10002 t
ORIGIN      
        1 aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa gaaaatcaaa
       61 aactaatttt gcatatcaaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
      121 tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca
      181 gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag
      241 aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat
      301 ggatgacttg agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt
      361 cactgttgag catacaggga atacgattat cgatcttgct tttaattata tccattttgc
      421 aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga
      481 gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt
      541 gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg
      601 gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat
      661 gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat
      721 atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt
      781 cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga
      841 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
      901 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
      961 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
     1021 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
     1081 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
     1141 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
     1201 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
     1261 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
     1321 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
     1381 ccaaaacatc attgaaaaga aaataaaacc tagagacacc tggggaatgt catttggcta
     1441 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
     1501 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
     1561 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
     1621 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
     1681 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
     1741 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
     1801 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
     1861 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
     1921 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
     1981 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
     2041 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
     2101 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
     2161 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
     2221 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
     2281 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
     2341 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
     2401 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
     2461 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
     2521 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
     2581 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
     2641 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
     2701 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
     2761 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
     2821 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
     2881 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
     2941 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
     3001 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
     3061 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
     3121 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
     3181 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
     3241 atcagtaatt tatttgaaat tttatatcta attttcaatt atccgcataa acactataca
     3301 ttcaagcatt ttgaagtata gaaaaacact catcttacca cgaatttacc acgattgaat
     3361 gagccttgat atgaccgtaa aactactaag ggagatagca cgaaactgtg tatctcccga
     3421 tttttttata cagttgatta cttcactccg atttcaaatt tcaatattgc tttcatcatg
     3481 tctgtatgaa tttctccttt tagttcttca tcaacctgca taatgatttt accactttca
     3541 gagtggtaaa aaggacgcaa gcataattta gaaatataac cgtcataaaa cttcaaaatc
     3601 tctttaactg ccatttcatc tccgcttgac gctttgcaaa tcaattcaaa agagggatag
     3661 ccataatctt tactcatttc tttacttcct ttcgctcaaa agcattttta acttccttag
     3721 tcctctagtt cttctactgc aaatagcaga acgtgataca tggtacagtt ctgaaatttc
     3781 tctgtcgttc attccttgaa aataacgtaa taaaatcgca ctacgctgtt tttctgacag
     3841 tctatgtaaa actgtaccaa gtgtttcatc agatacataa attgtaaagt gcaagacttt
     3901 aaatgataca caatcataaa aacaggtatc aatcgttcca cgttcaactt gtttcgtttc
     3961 ggctatttca caaaacaata cttcacgttt agaatgtctt gcaagctgtc tgtttcgact
     4021 tttcactttg ttcttaatga caatcagcat taaagaattg aactgtaatc tgacgatatg
     4081 ctcgaaagaa gatgtcctca taatctcacc ccctttctgc taatgctgaa atatataaac
     4141 ggaataccgt ttataccctt tagaaataca gaaaaatggt aagaaaaaag cccacacaaa
     4201 acatatattt gtgcgagcct taaatactgc aatattactc tttcttttat aacactatcc
     4261 ttttagcaga tttcaaattt tcttttatat attgttttta gcctaacata ctatttaacc
     4321 ccttgtatta tatctctaac ttttgaaaaa ttctttttga atatattgaa atcatgattg
     4381 aagcaataga agctacaaac ataacaaccc atgtttcttg tataaataca tcgaataaaa
     4441 taccgtaaac catttgtcca ataggttgac cgcacatagc aactgctacc agcgtagcca
     4501 ttatctttcc tactaattgc ggtggtgttt gtgtctgaac aagagtgtaa atttgaacaa
     4561 caaacatagt agatacaccc atagccacaa agcacatcac tgtaactacc cagtaggaaa
     4621 ctattgcagg caattctaaa aatagcgaaa ttcccataag taaaacagaa acagaacaaa
     4681 taagtaacaa ggtataaagg ttttttagtt ttaactttga agcaacaaga gctgttaaaa
     4741 taccaccaca taaaccacct aatgataatg ctgattgtga aaaaccatat agtgtatctg
     4801 gcatttttaa aacttcagaa attaagatag gcagaccaac aattaaaaca gcacttaaaa
     4861 ccaaattgaa aatgcaaacc aaaaagatta cggatataaa aatgggtttc tcctttttca
     4921 aaaaggtata actatctttt aaatcgtttt tagcaactgc aattacacct acttcacgag
     4981 gtcttttttg gtgtggaata tggataaaaa tttccataat ggctgaaaaa gtaaaacata
     5041 taatactaac aattaaaatg ggattgatac cataaaattg aaaaactact ccgcttatag
     5101 ctggtccgat aaagtttgct aaagtattta cttgatttat tatagcatta ccgacggtaa
     5161 gtttatctga tgataccaat agaggaatac ttgcttgtac cgctggttga tatgcaccag
     5221 aaataccata aagtaacatc aaagcaacaa taaacaaagg tatgatagga atttttccca
     5281 atgacaaata aaatactaaa attatgattg ctgttacaaa atctagtatg accataatgt
     5341 ttcttttatt tgtcctgtca gcaataatac cacctaccag cgacaaaata accataggta
     5401 aaaaagagca ggctgtagca ataccaaaca aagctgatga accagtttct tttaacaaat
     5461 agtacggtaa tgcaaatcgt aggatagcgt ttccaaataa agaaattatc tgtcctataa
     5521 caacaagtat aaaatcacga ttaaaaagtt ttttttctgc ttgcataaaa tctctccttt
     5581 ttatttaatc aaacagaccg tcggtttata taatggttta aataaactgc tcttttatag
     5641 agcagtctat cgtataggga taacaaatac agttatttac aaaactgtat aaatgcagaa
     5701 attgtttctt cgtctaataa tgtgtctatg cctattccat ttaaaagggc attgaatgta
     5761 ttgcaacgtt tttccatacc ctctttatcc gtcatttcaa ctaatgggtt gagccataca
     5821 ttacttaata ccatgataac ttctgcaatt tcatgtgaat tttcaatctc caaagactta
     5881 tccttgatac cttgttctaa aacgggttca acaaattcag gtactacaat ttcatacatt
     5941 tgttctattt gcatagcaag aaaacgagga ttgtttaata gttgaggaga aactgtcaat
     6001 aaaacgcttt ggttagaatt gaataccgct gtcttaaata tttctcttaa cttttctaaa
     6061 ccgtttaaac tatcgttatc tctaatttta gcaaatatta ctgcattttc atttccaatt
     6121 ctttgaaaaa ttgcttttaa aatatcttcc tttgaatcga aatgatgata aatagcacct
     6181 ttagaaagtt ttgtttcatt gataatatct tgcaatgatg tgttatcata tcctttttca
     6241 caaaacattt ttgtagaaac atctaaaatg agttttactg tttcttctgg atatttattt
     6301 cgtgccataa cttacctcca atacaaaccg ttggtctgat taaagtatat cttagtattt
     6361 tatgcttgtc aatataaaga cttgcaattt tataaaaatg cagaatgcaa gcctttttca
     6421 tttattctac aatcttccag ttgctttcct tatgcggtgt caattcatac tgtgatacct
     6481 gcgttgcctt tgcctgattt accacaaatc agattttatc cctttcttcc tcttgattgc
     6541 ccacaacaga aaagaaaaag ccctgtcaag agctttgcca ccattggtgg tgctttgcac
     6601 tcttgatcgg gctttttgtt tgctgtatct tctcgcaaga ggaaaaaagt tactcgtcct
     6661 catcatcaaa attgttattc cttaatactt ctcgtaacag ttccatatct tcttttgagt
     6721 tcgggtttat cattttcacg acttgataag gtgtcttata cttatgtccc tctatgcttt
     6781 ccccaaacat atccatactg tataaatcat catagcggtc taataatttt tgaaacttca
     6841 aatgctggat aaattctttg attgggtaaa ggtcggacgc ttcaatgctt ttcccgttga
     6901 tatagtctgt aatatattcc cgtaactttt ctaactcata ttccagccat tcttcctcgc
     6961 tgtcaaagaa gttatcatag tgtccatgtt tcagttctgc atttagacgt tcttcggctc
     7021 ttaaaaactg acagacttcc ttttcagatt gattgacata cttccattct cccgacaaca
     7081 attcattggg cgtaacgtcc agcacttcca ttatcttttc cagcgtttca taggcaggat
     7141 agttcaaccc tctttcaatc ttggaaaggc tctgcatatt gataccgatt ttgtccgcaa
     7201 gttcctgttg tttcattccc ctaaattttc ttatggtctg tatattattt cctaaatggc
     7261 ttattttcat tgcataaccc tccatatctc gttatatctt cgctattata agtctatcat
     7321 gcttaatatg atttgtaaag gcaaaaaaag atattgacaa ataagcatat aagaattact
     7381 atgttcttgt aatcgaataa gcacaataga atgaaatata aaaacattca aaaagaaata
     7441 cctatctgat taaatccgta gatatgaata tcggttgaaa tggaacttgt tagaaaaggc
     7501 aaaacagaac ttgctgacaa agtatttctg acacggtggc ggaagcctct caggcttacc
     7561 gccatgacac cttgtgtcaa ggggaagaaa tgctttgtcg gctggcgagc ctgcgagcct
     7621 tgtctgtgcc cccgttatct aacagggggg cactatttca caaaagacaa gtcaaaaacc
     7681 atacaccccc aaacctgtga gtagtaatga gaggttttaa gaagatagta catatcacac
     7741 tttttgaaag gtggtttaca ggtatttcaa ggctattgtg ggattggagg aatacaatga
     7801 acaatttaga atttgcactt gaaatgaaga acaaacgtct tgccagcggt ctttcacaag
     7861 gagaacttgc aagtcttacc catgtatcaa gatatagtat caaccgcttt gagaacggaa
     7921 aagctaacgc cagcaaggaa acacagaaca ttatcctgcg ttgtctgaat tactatatct
     7981 gcgataagcc tttttatctg ctcgttgatt atctgtctgt ccgttttcca acgactgacg
     8041 cattggaagt tatccgaaag gtgcttggca tgaaagcaga ttattttatc cattatgact
     8101 atgggtatta tggctataag gagcattatg cttacgggga aatcaaggtc atggcttctg
     8161 atgatgaaca catgggcgta tttttagaat taaaaggggc aggctcacgc aacatggaat
     8221 atgtcttaca ggcacagaac agggactggt attcattctt aaaccgctgt cttgactgtg
     8281 gcggtgttat ccgacgtttt gacttggcaa tcaatgatat gtgcggtctg ctggatattc
     8341 ctgtgctatc tgaaaaatac agaaatggtg gtgcggaatg tcgctgtaaa aactatgaaa
     8401 atgtacaagg tggaaaatta agcggaaaaa accgtaattt agcaagtacc ctttatatcg
     8461 gctcaaaggc aagcacaaaa tatttctgcc tgtatgaaaa acaaaaggaa caggcaacga
     8521 aaaagaaaca taccgatatt atcaaccgtt ttgaaattcg tctgcgtgac aaaaaggcgg
     8581 tacaggcagt tgaagaactg ttattgacgt ataaccctca tgggctggtg ttttatctca
     8641 ttaccgattt tgtggaattt cctgactatc cattgtggga gatattcatt tcccatgaca
     8701 gtttaccttt tgaaatgaac cctgtacctg tcaatatgga acgcactctg caatggctgg
     8761 aaagacaggt catgccgtca atcgtgatga tagaggaaat cgacaggctg acaggttcaa
     8821 actacatgaa aatgattgat gaatgtaccc acctttctga aaaacaggaa atgcttgtgg
     8881 aacagatgtg tacggacata tcagacgtaa tcgaaagtga gggggtgttt tatgagtaat
     8941 acacaggata ttcccgtatg ggaaaaatac acccttacca ttgaagaagc gtcaaagtat
     9001 ttccgcattg gagaaaacaa gttaagacga ttggcagagg aaaacaagga cgctggctgg
     9061 ctcattatga atggcaaccg catacagatt aaacgccgac agtttgaaaa ggttattgat
     9121 aaattggacg caatctaatg caaatgagcc ttgtatgtgt tatgatgaac acaagtcata
     9181 tcaaggctct ttccaacaag gaaaggagca gacaccatga aagaaaaaag acgggataac
     9241 aaaggtcgta tcctgcatac tggagagagc caacgaacag acggaaaata cttatataaa
     9301 tatgtagacg cattgggaaa cacaaaatat gtgtatgctt ggagattgac acccacagac
     9361 ccgacaccaa agggaaaacg ggaaaaaccc tcacttcgag aactggaaca gcagataaga
     9421 cgggatattg aggacggtat cgacagcaca ggcaagaaaa tgacgctttg ccaactctat
     9481 gccaaacaga acgcacagag gacaaatgtg aagaaaagca cacagaaaca acgggaacaa
     9541 ctcatgcggt tattaaaaga ggacaagtta ggtgctagga gcattgatac gataaaaccc
     9601 tctgacgcta aagaatgggc gttacgcatg aaagacaaag gcttttccta caataccatt
     9661 aacaaccata aacgctcgtt aaaagcgtca ttctacatag ccatacaaga tgattatgtg
     9721 aggaaaaacc cttttgattt caagctaagt gaagtcatag aaaatgatac caaagagaaa
     9781 gtcgcattga cagaggaaca ggagcaagcc ttattgtcat ttattaagac ggacaacgtg
     9841 tatcacaagc attatgatga tgtgctgata ctgttaaaga caggacttcg tatctcggaa
     9901 ctgtgcggac tgacagtagc cgacattgat ttcaagaatg aagttgtgat tatcgaccac
     9961 cagttactaa agagtaagga acagggctat tatattgaaa cgcctaagac gaaaagcggg
    10021 ataaggcaag tgccattaag caaagaaacg atacaggcat ttcaacgggt tatcaacagc
    10081 cgaccaaaga taaagtcgat agaggtagac ggacacagcg gttttgtgtt tatcaatccg
    10141 aaaggtaatc caaaagttgc cattgattat aacgccttat ttatccgtat ggtaaagaaa
    10201 tacaacaagc accacaagga caatcccttg ccacatatta caccgcatac gctacgccat
    10261 acattctgca caaggctggc aagcaagaac atgaacccga aagatttaca gtatatcatg
    10321 gggcattcaa acatcagtat cacaatgaac tggtacgctc atgcgtccat agataccgca
    10381 aaatcagagg ttcagcgtct aatcgcatag aagtatttac cacgatttta accacgattg
    10441 acagggaaaa tataagaaga tagacctaga tatgcgaggt ttaccacaaa agcaaaatgc
    10501 ccgtagagcc gataaaataa ggctttgcgg acatttgaga agatataaaa agatagtcaa
    10561 aaagacatat ataatttata ataattatga ctcttaatta aagtaggtgc ccatatgctt
    10621 gtttctttta gtatggttat aagatttttt ctgacaattt tattatcact gatattaaca
    10681 cctgtattca aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga
    10741 ataaataaag ttccaatgcc ttcagcgggc ggactgccta tctttatttc atttgttata
    10801 tcgactttat tcttattccg taaaattatc cctcaatcgt atattgtacc gatcttgatt
    10861 gcttctttag taatcatagt cacaggagtg ctcgatgata aatatgagct gacacctaaa
    10921 caaaaaagta tagggatctt gattgcttct cttattattt attttgtagc agatatacgt
    10981 attgattcct tcacgcttcc ttttattgga tatatccaac taggctggct tagttttccc
    11041 gtaacgattt tttggatctt cggtatcacg aatgcggtca atctgattga cggtttggat
    11101 gggctggcgg cagggatttc attgatcggt ttaattacga taggaatcat tggatatttt
    11161 ttcctccatg cttctacagt gtatattcca gttgttattt tctgtcttgt agcaagtatt
    11221 atcggttttt ttccttacaa tttttatccg gcaaagattt atttaggcga tacaggagct
    11281 ctattcctag ggtttatgat ggccgtcctt tcactacaag ggctgaagaa cgtgactttt
    11341 gtttcatcga tttctttgct gatcgtcatg ggagtgccgg taacagacac gttttttgct
    11401 atcattagga gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa acatcttcat
    11461 caccgactaa tcagtttagg ctttactcat aaaggtgctg tcctgacgat ttatgcgatg
    11521 gctttgatgt tttctttcac agctatggtc atgaattata ctggaagaat cggaacgatc
    11581 atattgatta ttgccatgtt gtttgctgca attttattat ttgaattgat tggtctgatc
    11641 aatgagaaac atcagtttat tttaaaaaca ttgcgatttt tagggaataa agagtaccgt
    11701 acgcaagtaa aacagaaata tttgaagaaa tggaaccgtc atatagatga ataacaaatt
    11761 aaaccagccg atccttatta gtattgtgac ggcaaacagt gataaaattt ttcggacact
    11821 agataatttt atttcaactg taaaagatga aacagcagta aaaatccgaa tttttgacaa
    11881 caattcactt cccgagtaca aaaaaagact aacagagtat aggaaatatc cctacatcga
    11941 tatctatttt catgatgaaa attccggatt tggttatgga cataatcaca atctgatagc
    12001 tagcgattct cgctatggca ttatttgtaa cccagatatt ttagtggatg aagagacagt
    12061 tacagcattg gtcttgttat tgaaagaaca tccggaatgt gcgatgttag cacctaagat
    12121 tcttaatgaa gatggaacaa ctcagcacct catccgtgag cagctggctg tttttgatta
    12181 cgttttgcgc tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac gactggctgt
    12241 gtttgaatgc cgaggattgc ctactgatgc taatagttat gtacggatga tctcaggtag
    12301 ttttatggta gctgatattg ataaatttaa agagataaag gggtttgatg agcgtttctt
    12361 catgtacttt gaagacaacg acttgtgttt gagttttgaa aaagcaggtt acaagttatt
    12421 gtacacaccc ttttatgcag ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa
    12481 actgtttcgc gtgtttttga cttctatgat gaaatttttc aataagtggg ggtggcgttt
    12541 tttctgatga aaccagaaat attagtagtc gttgtgcttt atcagcaact attcagtcaa
    12601 tctccagctt atggaccatt aagcaaagca cttagcgaaa agcaaattca gcttattatt
    12661 tacgataata gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat
    12721 catcatgatc cgcaaaatcc tggtttagcg gtggcttata attttgcgtt gtctcaagca
    12781 gatgaaggaa cacgttgtct cgtcactctg gatcaggata cgaggctagt cgacgattat
    12841 tttgaactat tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt tccgatgatt
    12901 ttttctggga gccgacaagt ttctccccta tattcagatc gttatatcaa tcgtacagct
    12961 caagcagtag aagttgaagg cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt
    13021 gcctggtctg tcaaatttct aaaaaagatt ggcgggttta atcctgcttt ttctttagat
    13081 tttttggatc attggctgtt ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt
    13141 cctgtacgaa tggaacatga tctttcggtt ttagactatg aaaaagtttc aagtaatcga
    13201 taccgttcta ttttacaagc agaaaatcga ttttatcatg aatatgatca aaggcattta
    13261 tccagtcacc gacgacaatt attgttaaga acagctaaac agttcttgac cgtcaaaaac
    13321 cggcatatct ggcggatgac tttgcgctca tatgttaaga attggaaggt gtgaattcgt
    13381 gcgttcggtt tgtatagcta cctttaatgg aggcagatat ttaagagagc agttagatag
    13441 tattttatct cagctttcag cagaagatga agtagtcatt tctgatgatg ggtcagtaga
    13501 tgatacatgg gatatattaa ctgaatacgc agaaaaagat aagcgaatcc agcttttttc
    13561 ggggcctcgt caaggattga tcgctaattt tggttatgcg atccagcaga cgaaaggaga
    13621 tctaatcttt ttggcagacc aagatgatgt ttggttggag acaaaagtga aaaagatcac
    13681 tgactatttt gagcagaatc cggatcagct agtagtagtt tctgatttaa tcattgtgga
    13741 cagcgagctg caagagatcc atgattcgta tttttcgttt cgaaattcca aagaaggatg
    13801 gcttaataat gtgataagaa gtcactatat tggtgctggt atggctttcc ggtcttcttt
    13861 aaaacccgat attttgccga tcccgtcaga agtgccgatg catgatatgt ggattggttt
    13921 gctgggagga aagaagactg ggttcctaag agaaccgtta actctgtacc gaagacatga
    13981 tttaaacgcg agtgagattg aaacacattc taaattacgg caaaaaattt cttggcgtgt
    14041 tcatctgatt acggcgttga tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa
    14101 atccttaata tttccatgac aaatataaca attgatgaga taataacaaa gtgaaatttg
    14161 aaatcaaaaa ggagagcaga tatccatgaa gggaatcatt cttgctgggg gcagcggaac
    14221 acgtctgtac cctttaacaa aggcaacatc gaaacaattg atgccgattt atgacaaacc
    14281 aatgatttat tatccaatgt ctactctgat gttggcggga atcaaagaaa ttctgattat
    14341 ttcaacgcca caagatacac cacgtttcaa ggaattattt ggaaacggaa atgatttagg
    14401 actgcagatc gaatatgcag tacaagaaag tccagatgga ttggcacaag cgtttatcat
    14461 tggagaagaa tttattggag atgatagtgt ctgcttggta ttaggggaca atatttacta
    14521 tggtggcggt ctgtctaaaa tgttgcaacg agcagcttca aaagatacag gcgcgacagt
    14581 atttggctat catgtcaatg atccagaacg ctttggtgta gtagaatttg atgagaatat
    14641 gcaagctttg tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg
    14701 cctttatttt tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg
    14761 tggagaacta gagattacgg atgtcaataa agcatatttg gataaaggaa aactatccgt
    14821 agaacttatg ggacgcggat ttgcttggct tgatacagga acacatgaat cattgcttga
    14881 agcatctacc tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga
    14941 agaaattgct taccgtatgg gctacatcga taaggaacaa ttgctggccc ttgcacagcc
    15001 attgaaaaaa aatcaatacg gtcaatattt attgaatttg gccgctgaat aaggagtcct
    15061 tacagatgaa agtaacagat acaaaattaa cagatgtcaa aatcgtagaa atggacgtct
    15121 ttggcgatca tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt gcagaacatg
    15181 gattgaaata tgactttgtt caagacaacc attcactatc agctgaagct ggtgtccttc
    15241 gggggcttca tttccaaaaa ggagaagctg cacagacaaa attgattcgg gtagtcactg
    15301 gagcagtttt agatgtagta gtggatatcc gcaaaggtag cccgacttat ggtcaatggg
    15361 acggatttat cttatctgaa cataatcatc gacagttgct tgtacctaaa ggatatgccc
    15421 atggattcgt tactttgaca ccaaatgtga acttccttta taagtgtgat aattactata
    15481 atgcagaagc agatgggggc atcgcatttg atgatccaga tttagcgatt gattggccaa
    15541 ttgatccatc aagagcgatt atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat
    15601 ttgaagcaga aaatccattt gtttacggag aaatctaaga attaaggaga aattttctta
    15661 tgaaaaacat cattgttacc ggaggtgccg gattcatcgg ctctaatttc gttcattacg
    15721 tggtaaacaa tcatccagaa gtgcatgtca ctgttttgga taaactgact tatgcaggaa
    15781 acaaagagaa cttggctgga cttcctagcg atcgcgtaga attggtcgta ggagatgttg
    15841 cagatgcaga attagtggac cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg
    15901 cagaatccca taacgataat tcattaaaag atcctttccc atttgtacaa acgaatatca
    15961 ttggtacgta tacactttta gaagcttgcc gcaagtatga tgtacgctat catcacgtat
    16021 ctactgacga ggtttacggc gatcttccat tacgtgaaga cctgccagga catggggaag
    16081 gcgaaggaga aaaattcact gcggaaacac catataatcc ttcaagtcca tattcttcta
    16141 caaaagctgg ttcagatctg ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa
    16201 ccatttcaaa ctgttcaaac aactatggtc cttatcagca tattgaaaaa tttattcctc
    16261 gccaagtaac gaatatctta agcggtattc gtccaaaatt atatggcgaa ggaaagaatg
    16321 ttcgtgactg gatccatacg aatgatcatt cttcagcagt gtggctgatt ttgacgaaag
    16381 gcagaattgg cgaaacctat ctgatcggtg cagacggtga agaagataat aagaccgtga
    16441 tggaattgat cttagaaatg atgggtcaac caaaagatgc gtacgaccat gtaaacgacc
    16501 gtgcaggaca cgacttgcgt tatgcaatcg attcgacaaa attacgggaa gagttaggat
    16561 ggaagcctga gtttacgaac ttccgtgatg ggttggctga cacgatcaaa tggtacgaag
    16621 aacatgaaga ctggtggaaa aaagaaaaag aggccgttga agcagcatat gccaaaaatg
    16681 gacaataata cagagaaaat gtcactgtat taatggaaaa acacatgtga ggcaggcgct
    16741 cgcctgtctc ttttttaaaa taaaaatcaa ggagcggata atcatatgtt tttattaact
    16801 ggaggaaatg ggcaactagg tacagaattg cgccatttat tagatgaaaa aaatctgcaa
    16861 tatgtttcaa cagatgcaaa agacttggat atcacagatg ccgagaaaac aatggcttac
    16921 atcacagaat taaaacctga tgttattttc cattgtgcag cttacacagc agtagataaa
    16981 gcagaagaag aagcaaaaga attagatgag aagatcaatg ttgatggtac aagaaatgta
    17041 gcacttgcag caaaagctgt gaatgctcaa ttcgtctata tcagtacaga ctatgttttc
    17101 gatggaaaaa acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc actaaatgaa
    17161 tatggtcgta cgaaattatt aggcgaaaaa gcagtagaag agattttaga agactattat
    17221 atcatacgaa cttcttgggt tttcgggatc tatggacaca atttcgtctt cacgatgcaa
    17281 cgcctagcgg aaacccgcga caaactgact gtagtaaatg accagtttgg gcgaccaact
    17341 tggacacgta ctttggcaga atttatggta tatgtcatcg aagaaaaagc accatttggt
    17401 atttatcatt tgtcgaacga aaatagctgt tcttggtatg aattcgcaaa agaaatattg
    17461 aaagatacag atgtagaagt tgcacctgtt acgtcagaag aatttccgca aaaagcaaca
    17521 cgtccgcagt attcagtcat gagcctgaaa aagacagaag agttaggatt tgtcatacca
    17581 acttggcaag aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt
    17641 ccttttatca aatagaattt ggagcatgat atggaaaaac aaacaagaag tgcacgacga
    17701 aaagaaaagc caaaagcgaa ccaggcagat tcgaatttgg tgcttgacct gtttctaaaa
    17761 ctccgctatc ttattggcgt tttagtcatc gtggtcatcg ttacttttaa tttaaacgga
    17821 agttcacttg gtgtttggga taaatatgtg tcgcaaagag acgatgggaa aaaaactgat
    17881 gtgatttttg gacagaatcg ggaagtgcgg tcagacgaat ggctggtgca gacacctttt
    17941 tacctctcac aagctgaaga ggactttccg ttggtgaacg aggattattc attaaacgga
    18001 caaaacatga tcattgcgta taattctcct gtaaaggata ttacggttat cggaaaacca
    18061 ttcaactggg gattcttttt cctcggaaga gatcgaggac tttcctttta ttgggcgatg
    18121 aaattagtcg tcatggtctt gcttggattc gaagtactga tgatattaac gaagagaaaa
    18181 aaagctctat cgctaatcgg tgcatttgcg ctgacatttt ctccagcagt ccagtggtgg
    18241 ttcatgcaac atgtgggcga tttgattttc tttactttgg gactgatgac tgctttttat
    18301 cattatttct acaatcatga taaaaaatgg ctacgaacgt tgatgatgct tttggtggtt
    18361 attttcggta ttgggtttat tttagtgatt tacccagccc atcaagtgat gctggcgtac
    18421 ttgctgattt tttattttgt tggcctgctt atttactata gcaagaaagt ttcatgggat
    18481 ctgttggatg tagtgttgat tggaggggca gttttattta ttggcggagt catggttcat
    18541 ttctggctaa cgtcaaaaga cgcattgtta gcttctttga ataccttata tccaggaaaa
    18601 cgggtaagta ctggaggaaa gtggacgatt ggcgaatttt tttctttcct tacaaattgg
    18661 aaaattcctt ttgaagatat caactattcg aataactctg aagtggccct gttctatcac
    18721 ttttttccaa ctgtattcct agcaagtcca tttgtgttat ttggcaaaaa agatagcgaa
    18781 caaaagttac tgggacgtat tttgatgatt ttttgtctgt tttctatctt ttggattacc
    18841 gttgggttgc caaaatggct agcagaaatc actctgctgt cttatgttcc tccattaaga
    18901 gccatcctta cttttagttt tgcggcttgt ttattatcga tctggtttat cgcttatatt
    18961 tggagagaag atgttattcc tatttggtat aaattcattt tgttggcagt aaatgcatgg
    19021 tcttatttct atgcgattac gcattcgaaa atggataatt atttttcgga ttttgaaacc
    19081 atcgcagttg ctgtgttggg aacagtgatt ttagcttttg cattatttgg ctggagacgc
    19141 ttgttttacc tcgcttttgc cgccttagtt atcgtatctg gctttttcgt caatccagtc
    19201 gtagaaggta caggagcgat ttttgaaaaa acactggcaa aagaaatcca aaaaatcgat
    19261 caacaagatc ctggtaaagt ttggttgtct gaagatgaat tatataactt caccccaaca
    19321 ttgggagtga aatccttcaa tacagtacgc ttttatccag atatagctgc atggaagaaa
    19381 atcgatccga aagaagaata cgagaagtat tataatcggt atgctcatac aagagctttt
    19441 atcgctaagg aagatacaaa gttcatttta gatcgtccag atatgttttc ggtcacgttg
    19501 aattttgaag acgcagaaaa attagggatc gactatgtga tcagtaaacg accattaact
    19561 gattacaacc aattatatga agcgcagttc acgcagctat atggaccgga taaagacggt
    19621 tatatgattt ttaaagtaga atatccggat tatccagatg taccacaaac acaaactcag
    19681 gaaaatcagc tagtcgtaca gtaataggct agctgagaat aatcctgaaa tgttagtaga
    19741 aaggagagtg gagataattc aagcaatcgt acaaaaattt gtcaacaaaa tgagtgtcgg
    19801 tttcttgctt ttgttactga cgctagtctt ttttgtttgg acagcagggt taggttttcc
    19861 agcaactttg tttatatata acaatagttc attacttatc gcttttagtg tggctatttt
    19921 gctcatttta aatgtttata aagcaaaagg cgtcgatttt ctttatctag gtgttgcgct
    19981 tgcccttttc attttctttt cttactttcg ggaattaaga aatgagtctt tatttggaga
    20041 cttactgttt cctttgatta ttttattttg tttagtaatt aaatggatcg agtggacaaa
    20101 atgggatcgc ggtatttatc tgattgtctt ttcggtcttc ctcggaatca cgttatatcg
    20161 tgtatttaca gagatccaag taccagaagg acaaagcatc tgggctccag gcaattcgct
    20221 ttctgatatt tggatcaata cgaatataat cggaagttct ttgatgactc ttgctttatt
    20281 gatcagtgga tttgccagtt cttttgaacg atggtatgta cgaatcatcg gtgcagtagc
    20341 agttgttgca gcttttctcg ggatttgggt ctgccaatct agaggtgcat tagtcgcgtt
    20401 gattgttttt gcgatattgg atcttttacc taaatcgttg atgcgtgtca ttcgtgcgcc
    20461 atttattgct tacacggtta cgattctttt agctttgccg atttcttatc tggcggctgt
    20521 ctctgaaaaa gtcaatttgt ttaccggtcg agaagatatt tggcataaat tctatcagac
    20581 gctaggagaa aaatctgaac aaattttact aggaatgaaa acatttatct tccaacgagg
    20641 aaaccaattt ttaggaaacc acaatagtta taattctatt ttgaatatct atggattgat
    20701 aggatttggg attgctgctt tgctgcttat cctttttatt ggtcgcttaa cgttgaaagc
    20761 agatttatcc aacgggcaga tgacttttat ctgggccttt tttgcggtga tgatgcagtc
    20821 atttatggag gatacattga cttcataccc atgggttccg attatttata tcctgttggc
    20881 tatggccgtt catcgatatg atgaaccaga agttgcggag gaattctatg ttatggaaga
    20941 ggcatatgac gatatgactt atgatgatac atcttatgag gaacaaccag atagcgctca
    21001 ttcttccagg atccaacgct atcattgata ggcacaaaat acgagagagg ctgtgacaac
    21061 cgtcgcagcc ttttttggtt tattgcaatt gtctagcatc ttctgtaaaa tagaaacgtt
    21121 agagaaaatc ttgtatcata gaaaattaag aaagaaataa gaacgcagct cggtaccaaa
    21181 aatatggtaa aatggcactt gactgtaatt cactgaaaat cgcttggagg aacacaaatg
    21241 tttaaagatt tgtatttatt tatgaaggac atttatcaga acagaaaatt attgctacag
    21301 ttttcgatca atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg
    21361 tttatcaatc cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa
    21421 gcacaaatga tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc
    21481 tggttcttct tctcagatgc atttgcatca actacggttg tttttcggga atatacttat
    21541 ttagtaaaga aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat
    21601 ctgtacaccc acttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata
    21661 tatccttcag caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg
    21721 acaggattga cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta
    21781 attacgatcg tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag
    21841 tttgattcag gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt
    21901 tatcgtgagt catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat
    21961 ttctgggttt tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa
    22021 ccacattttt cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa
    22081 aagatatcac aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc
    22141 tcaatccttt taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag
    22201 aaataaaaaa aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa
    22261 tgttgaaaat cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa
    22321 aaatctcagc tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa
    22381 atatctattt aaatggaatg gttctaggat tttcaagaga agaagtcgat gaaatggtcg
    22441 atgatatcat tgaatttgct gacatcggag accatattta tcaacctgta aaaacttatt
    22501 caagcgggat gttcgttcgt ctagcttttg ctgtagcaat caatgtgaat cccgatattt
    22561 tgattgtgga tgaagcattg gcagtaggcg atcttgaatt tcaactaaaa tgtatggaaa
    22621 aatttacaga aatcaagaac tcgggaaaga cgattttatt cgtttctcat gatatcagtt
    22681 cgattcgtcg tttttgtgat cgttcctatt ggttgaaaaa cggagaagtc gtcgaatatg
    22741 gcgatacgat ggaagtaacg acgaattatg aaaacttttt aaagaaaaaa tcagttaaaa
    22801 cagtggatcg gaataaaaac caagtagaac ggttcgccgc tccagatatc gtcgacgtga
    22861 cgaaagcgga attgttggat aaagacaaaa atccggtgga tatgctggaa caaggagaaa
    22921 aactatacgt taaagtaact tatgaagtaa aagatgatac aatcaaaaat ccggttttag
    22981 gaattgcgct acgaacagtc gacaattttt atgtatgcgg acttaatact ctactagatg
    23041 gtatcaagat tccttggaaa aaaggagaga atatctttta tctggagtat gaaaagttag
    23101 gtttgttagc tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc
    23161 ctttggtata taagacaaag tacatgaact tgtttgtcag cggttcttat atcggagaag
    23221 gcatcgtggt cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt
    23281 gaaatggacc tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga
    23341 agtaaagttt tagagtttgg tcccggaaac ggccgattaa cgaaacattt aatcggagca
    23401 aaacaatgtg aagtgagcat tgttgagtta gataaggaat tgttcgattt tgtcagcgaa
    23461 tttgctcaag atggatttta tggtgatatc gaaagttttg agtgggcaaa ctattatgct
    23521 ggacagacat ttgattacgt cttgtttgcg gatgttttag aacatttggt agatcctgga
    23581 aaaacattga agaaagtcag agaatttttg aatgaagaag gagagatcct gattacattc
    23641 cctaacttgg cgcataattc tgtcatgatc gatttgttca ataaccaatt gccgtgggct
    23701 tcttatggtc tcctagatga aacccataat tctttttata ctcatgatgg cttccaaaaa
    23761 gtttttgaaa aagcgggatt attcattaat attgaagatt atttgtactt agctgtaggt
    23821 gatacagaat taaaatcaac ttatgaagaa ttgccggaag ctgtacgcta tgatttcaaa
    23881 atgcgaccat ttggtgaagt ctaccaatac tttttctcat tgatgaaaca tccagtagca
    23941 caaagttcca ttgcagagcc acaaaattct aattatgtaa aagtgttaga agtaacccag
    24001 caaacgaaac aagatgaaac aatccaacaa ataccattca ataatttcac aggggaaaat
    24061 gagacactaa gtttccccgt atcagaaaca acagaaagaa tggtgtttcg cttcgcgcaa
    24121 caacctagct ttatcgaatt cagcgcagaa gctgccggag aaaaactaac atttatcgat
    24181 tcgaatgctg tagtcaaaac agtaaatgat tgttatttgt ttgatgggaa agaattacca
    24241 gagttcgttc taacggatat ttctgggaaa gaagtgacta tccattgtca ctatcgcttt
    24301 atcggggaat tgacaccaac aatgaaagaa ttgttggaaa cgattcgtcc catggctgaa
    24361 gtcacaaagc agctttctga gaaaaatgac gaattagaaa gagaaaatca tcatcttctt
    24421 gaggaaaata caagactaga ccatacgtta aaaacaacca caaatcgcta ttgcacattg
    24481 ttggaaagtg atgaatggac aataaaacat agactaggac gacgtaagaa agaaacttcg
    24541 aaaaaaatcc aagaaaaaga actttctata tgtattgacg agaaaatctg ggatgcagaa
    24601 acaaaaatat taaaaatcat cggatgggga atcgctaatt cggatcgcca gcccttgtcc
    24661 tataagttat cagctgacca atcgccattt ttcgaagcaa ttcctgtttc acgagaagaa
    24721 gtcaatcaag cagaacagct cgcaaaagga acagaagcgg gatttgagtt acgaatcctt
    24781 tgcgagcaag aacgttcatt cttagtggaa gcagtagctc aaaatggcca atcatggatc
    24841 attgaaatgt aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa
    24901 aagaaattgc cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta
    24961 ttgtgggctg ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag
    25021 aaaatgtgat tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc
    25081 ttgatgttaa gacccaaagc ggttttaagg tacgtttatc agggaaaatg agaggaaaag
    25141 ccatcttaga cttccaaaca gcgagacatc aaaatggcat agctgtgaaa ttaaacggta
    25201 aataccctta tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa
    25261 aagggataaa ttatgcaagg actcatggcg taaaaaaagc gatccgtcgg gtcaaattgg
    25321 aattgaagcc aggatcgatc gactatgcag agtggatcag tcgtcatgag aaaccagatt
    25381 tgaaagagca aaggaaagaa gcacaaggat ttgcgtaccg tccattgatt tccatactta
    25441 tgcctgtcta taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtactggatc
    25501 aaacgtatga tcattgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc
    25561 gaaagtgtct ggaatcctat caagcaaaag atgatagaat caaagtcgtt ttccgaaaag
    25621 aaaatggaca catcagtctg gcaactaatt ctgcattgga aatagcagaa ggagaattta
    25681 tagcactctt agataatgat gatgaattac cgccatttgc attatatgaa gtggcaaaag
    25741 tgctaaacgt acatccagaa ctggatgtga tctacagtga cgaagataaa atcgatgcag
    25801 atggcaatcg ctttgatccg catttcaaag cagactggtc gcctgataca ttgatgggaa
    25861 ataattatat ctctcactta ggcgtatatc gaacaagcat tgttaaagaa cttggcggtt
    25921 tccgtaaagg ctatgaaggt tctcaagatt atgatttggt ccttcgtgtg acagaacaaa
    25981 tccctgcaga tcatatttac cacattgacc gagtcttgta tcattggcgg acgatcccag
    26041 gatcaactgc aagtaatggc gaagccaaaa gttatatcta tgattccgga gtgaaagcat
    26101 tgacagatgc cctgtctaga agaaacatca aaggaagcgt acgtcctggc agaatttctg
    26161 gattttatga gattgcttat gacgtcttgc aagaggattt ggtcagtgtg atcattccaa
    26221 cgaagaatgg ctatgaagat ctaaaaactt gtgtggattc gatcatcgaa aaaacgtctt
    26281 attcaaatta tgaaatcatt attgcggata acggaagtac agatccaaaa atgcaagaat
    26341 tatttgcaga atacaagcat cagctgaaag accgctttat cgtagaattg attgacattc
    26401 cattcaatta ttctcgaatt aacaatttag cagcagaaaa agcaaacggc aaatactttt
    26461 tattcttgaa taacgataca gaagtgattg agccagattg gatgacggtg atggtttctt
    26521 atgcacagtt tgatcgaatc ggctgtgtag gagcaaaact gttttatcca gatgatacaa
    26581 cgcagcatgc aggagtatta ttaggaatcg gtggtgttgc agggcatgca ttgaataact
    26641 atgatcgaac acattgtggt tatttcggcc gattggtcat tgacgtcaat tacttagcag
    26701 tcactgcagc ttgtatgatg gtcaaggcag ctgacttcaa tgctgttaac gggtttgatg
    26761 aaacattaga agtagcattc aacgatgttg acttgtgctt gaaagtttat gaactgggtc
    26821 gttacaatgt ctatgcacat caagcagaat tgtaccattt cgaatctaaa tcaagaggtt
    26881 atgaagatac tccagaaaaa caaagacgtt ttgctggtga aatcaaaaaa atgcaagata
    26941 aatggccagc atatattgcg catgatccat tttacaatga taatttaaca aaagaaggaa
    27001 tcggcgactt ttcgttgaga ccagattaaa ctataataaa aagctgttat gttgtgattc
    27061 gctcgcacat aacggctttt tatagttttt tgtcaaaaaa aggcagaagg tgccgactca
    27121 aaaaaataag tcgaggttgg gacagaagtg tttaactccg agaaataaga agaaattcac
    27181 gaaaattgct tttcaaattt ttgtgaattt cggcttattt aagaagaagt tgcttctgct
    27241 cccaccgttt attcgttttt aaagaatgag acaaaagtgt ttcttacttt tgtcccacgc
    27301 tcttattcat gctcagagcg ccaatagata caaaatcata gccaaagttt cacgaaccct
    27361 agctgtcaaa gaatcgatat tttattttaa tataggctat tttaaaataa atggtttaag
    27421 gtattcctct acaaaagcca ctctatcaat tatcatgggt ttaaaataag aagtgatgga
    27481 gataaccaga ttgttcttag cgtcaatata aagaacattt ccactattac caattgctga
    27541 atatatttcg ttctcagaat ctaataccca ccataaatag ccatatctca tattttgtaa
    27601 gtcttctaaa gcactggttc taggtttgaa catctcgttt atccacatgg gtggtacgat
    27661 ttgttttcct ttatacatcc ctttatttag gcataataca ccaatttttg ccagatcact
    27721 tgccgataaa cacaaaccat atcctgccgt cccgatattt agtggatcac aaaaccagac
    27781 attggtttta ggagacttat ccattgtaaa ctgcttatgt tcttcagctg tttcagcata
    27841 ataattgaca tggtttggta tccctatagg atcaaataga tacttattgg caaaatcaac
    27901 agttgttagc ttacttgtct gatacaacaa gccagttaat atgtgtaaac agacagtttg
    27961 atagttgaat tcgccggtta taccttttct tcctcccaat aaatctagtg atgtttttgt
    28021 ccaatcttca cttgaacaga cttttgtcca tggatcgcct ttacatttgt agggtgcttt
    28081 cattgttagc aagtgcttga ttgtaatagc gtaaatagtt ttctccccgc gtttcacttt
    28141 atactctgga aaataatcta atactttatc atcgatactt ttgattaatc cttgctctaa
    28201 agcaatgcca atcagcaaag atacaatact tttcgttgct gacatgatat gacaagtatc
    28261 gtctttttta taaccattcc attcatctgt ataaacttct accccatcct tatatgcgac
    28321 aatttgacat atatttggtt gtcgaaaacc aataaaatca tgcaactctt gtttattcat
    28381 aagatattac ctcactttct tctataagca taatgtcatt aaaaatcgat ttcaattttt
    28441 tcgattcttc acgtaataca ggtattctta tggactagac agcgctttgg gaagatggat
    28501 tctctgaaaa atataaaggt tcactttatg agtggataaa aaaagcagca aatacttcga
    28561 cttctttaat ttaccaacat tagtgaaaat cgattcatct ggagatttgt aatttagagt
    28621 ccttgtcttg attagaggtg tataaagctt tttattagac atttttaaat tccattacaa
    28681 tttgcttata aactaggttt ttaaaggtga ctatggtaag ataatagaga tattattttg
    28741 tgattaggag tgtatatatg aataaaaatg gagaatggag tgctgcacga cggatattca
    28801 ttatattaat ggatgttatt gtatacaatg cagcaatata tagtagtttc ttactgaagt
    28861 ttcgtggaga gatcccatta cgaaatttag aaactttcca acactcagca attattattt
    28921 cagtgatttt tattgcgttg aacattttgt tgggtgcata tgttttttat aatcgaatga
    28981 tcagtgacat cgtgtttgtc acagtcatcg gtcaggtatt gatgacacta gggatcatgg
    29041 tagtgacttt tgcgggccgc tggtttgctt ttccacgtct tgttatctta ctttcatttg
    29101 ttctcggtac gcttttctta agtatctatc gtattctcgt atataaactt tatttacgcg
    29161 taagtcgaga taaaaaggtc gtcattattg ggttagaaaa ggatgtcgca ccagcaatcc
    29221 aaaattttgg ttcaaagaaa aacaacaaac ataaagtcga agcagtcatt cttgataact
    29281 attatgcaaa catcaaaaat atgattaaag acatcgatat tgtctatctt gcttctcata
    29341 tcgaagaaac tgaaaaaatc aaaatctatc aattagttac taaaaatgaa aaaaaattgt
    29401 ttttgaatac aacatttgaa aacttgacat tgatcaatcc aaatattatg aatttcgaag
    29461 atgaaagtat tattgaagca tctggattta gaatccaacc tgaatatgaa ctattcaaac
    29521 gtttgtttga tatcatcgtt tcacttgttt tattagttat tgcttcacca tttatgttat
    29581 tcacagcaat tttagtcaaa gtgacatcac cgggaccaat catctataaa caagtgcgga
    29641 tcacaaaaaa tcaaaaggaa ttttcaatct ataaattccg ctcgatgacc gccacagccg
    29701 aagcaaaatc tggaccagtt ttagctaaga gcaatgatgc acgagtaacg cctgtaggta
    29761 aatttattcg agctgtccgc tttgatgaat taccacaaat cttcaatgtg ctgaaaggcg
    29821 atatgtcgat cgtaggacca cgtcctgaac gtcctttctt cgtcgatcag ttcaatgcag
    29881 aaaaccctta ttactactta cggcataatg ttcgtgctgg tatcactggg tatgcacaag
    29941 tctatggaaa atatgtctct gattacaaca gtaaattacg ttttgattta ctttatatca
    30001 aaaaatattc attgatgctt gatttaaaga tcttgctgca aaccatcaag atcctatttg
    30061 ataaaatgtc ctctcaagga cttgaagaag acgaaaatgt gatctcattg gcagaaattg
    30121 cttttccgaa tgaaaaaatc tttcgctagt tgggaggcgt aaaaatgaac aatccgaaat
    30181 tcagcataat cgtaccagta tacaatgcag aagcaactat tggtcgaacg attgatattc
    30241 taaaagaatt ggattatgaa gagtacgaag tcgttttagt aaatgatgga tcaacagatc
    30301 aaacacagga aatcattcaa gaactgattg aaaatgatga acgttttgaa ttgattacta
    30361 cgaccaatca agggcccgga ttagcaagaa acgaaggaat aagacagtca aaaggagaat
    30421 atctcttatt cttcgatgca gatgacacgc cagtaaaaac gatcttgtca gattatgatc
    30481 atttgctaga aaagagtcca aaagcggatc ttgttgttag ttcgtttgtt tttcgtacaa
    30541 tggacgaggg aagaatcgtt tccgaaaaag aaaacttagt tacagaatgt cactatctaa
    30601 ctcacgaaga gtttataaaa gacatgtatc agttaatgaa tgaacagttg atgtatgttg
    30661 tatggaacaa atgctatcgt agcgatattc tgaaaaataa ccaaattcta tttaaaactt
    30721 ataatagttg tgaggatcga atatttaact tacattatta tgaatactgt catcaagtga
    30781 tcatgaaccc aaaagtagaa tatatttatg aatttgaagg tgggaagggg atcactaatc
    30841 aataccgacc taacaagttc gaaacattta aagaattcta ttcattagca aatactgtaa
    30901 cagacgatat taataaagac ggtatggcgg cgcttctttt aaaagggaca acttcagtta
    30961 ttttttctat ttatagtacc tctgcaagaa cttcaaaaga gaagaaagac gaagcaaagc
    31021 agatattgag tgatccagtg atccgagaag ccaagcaaat tgctaagacg gattcttcag
    31081 caaaaaaaat aacaaagttg ctttacaata tgccagctcc actattttta tctgctgtaa
    31141 aagttggaag cttcgttgaa gtgaaaatgc cagggttaat ggctgctttg aagcgagtat
    31201 attagttaat ggaggaaaat ataatgaaaa gaattttgca ttttcaggga cgaatgggtt
    31261 taggtggagc agagtctttc atgatgaatt tatatcgtaa aattgatcgt acaaactatc
    31321 aatttgattt tttgatttat gaagattatg cagatgttca agactatcat tcagagattg
    31381 aaagattagg aggccgtatt tttgttgtac ctaatcctaa gaaaaatatt tttaaatatt
    31441 taatagaagt gaataagtta ctaaaaaaag aatctttttc tatagttcac aatcaggttt
    31501 attttggcgg gggaattaat ttatggttag ccaaaaagaa tggaatcaga cagagaattg
    31561 ctcatagcca tgcaactgaa gatggaaaat ctcaaaatat tgtaatgaat gtattgcgta
    31621 aatttttaac caaattgttg ttacaaaatg caacagacta ccttgccgtt agtcaagaag
    31681 ctggtgaaag tctgtttcaa aaccacccct ttgagatagt tcataatggt attgatttag
    31741 agctttatag taaaaattca gaagctaagg ttaataagag aaaagaattg gatatctcaa
    31801 tgagtacttt tgttgtggga aacattggac gattagagaa acaaaaaaat caatcttatt
    31861 tgttagagat ttttttagaa atactaaaaa tgaattctga ttcttacttg attttagtag
    31921 gtgaagggtc acttagaaat aaactgaaag ccaaagcagt agaattggat atagagaaaa
    31981 gagtaaaatt tttaggagaa agaaacgata ttcccgagtt attatctgtg atggatgttt
    32041 ttgtaatgcc ttctctttat gaaggtctgc caatggttgg attagaagca caagcagcaa
    32101 aattaaaatt agtcttagca gatactattt ccaaagatac aaagttaaca aataatgtgg
    32161 attttgtatc attagatgaa acaccacaag aatgggcaaa aaaagtaata aaagagccgt
    32221 atgctaataa aataacacaa gaactaaaga gttatgatgt ttcgtacact actgaacaaa
    32281 tggagagtat atatagtaga gatcattaaa aatgttcaag atattttagg agtgattatt
    32341 tttgaaaata tgttatttga tattggcata caatgatatt ggcaacttaa agagattggt
    32401 caatcgattg aacgaaaatg cagtttttat tattcatata gataaaaaaa ctgatataga
    32461 accatttatt gaagcattta aatcatatga taatgttgaa tttttatgta ataaaaaccg
    32521 tgttaaaatt tattggggtg gtttttccat tgtacaagca gaaatcaatt tagtaaagag
    32581 agcattacaa aatgagaaat atttaaaata tgtattatta tc
//
LOCUS       DAZUMT010000019        32410 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000019 DAZUMT010000000
VERSION     DAZUMT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.019"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..327)
                     /locus_tag="ACG8FM_000826"
     CDS             complement(<1..327)
                     /locus_tag="ACG8FM_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093510.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIK"
     gene            complement(334..882)
                     /locus_tag="ACG8FM_000827"
     CDS             complement(334..882)
                     /locus_tag="ACG8FM_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS5093511.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(927..1259)
                     /locus_tag="ACG8FM_000828"
     CDS             complement(927..1259)
                     /locus_tag="ACG8FM_000828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093512.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1273..1530)
                     /locus_tag="ACG8FM_000829"
     CDS             complement(1273..1530)
                     /locus_tag="ACG8FM_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093513.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1725..2279)
                     /locus_tag="ACG8FM_000830"
     CDS             complement(1725..2279)
                     /locus_tag="ACG8FM_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFS5093514.1"
                     /translation="MTRNVKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIE
                     AVKETSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQA
                     VRDYMTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVL
                     LQLKVDQEAQKIDAIKIYYVRSAY"
     gene            complement(2288..3379)
                     /locus_tag="ACG8FM_000831"
     CDS             complement(2288..3379)
                     /locus_tag="ACG8FM_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5093515.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3387..5597)
                     /locus_tag="ACG8FM_000832"
     CDS             complement(3387..5597)
                     /locus_tag="ACG8FM_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5093516.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5613..8141)
                     /locus_tag="ACG8FM_000833"
     CDS             complement(5613..8141)
                     /locus_tag="ACG8FM_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5093517.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8193..8603)
                     /locus_tag="ACG8FM_000834"
     CDS             complement(8193..8603)
                     /locus_tag="ACG8FM_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS5093518.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8616..8834)
                     /locus_tag="ACG8FM_000835"
     CDS             complement(8616..8834)
                     /locus_tag="ACG8FM_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFS5093519.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8831..9823)
                     /locus_tag="ACG8FM_000836"
     CDS             complement(8831..9823)
                     /locus_tag="ACG8FM_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5093520.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9902..10813)
                     /locus_tag="ACG8FM_000837"
     CDS             complement(9902..10813)
                     /locus_tag="ACG8FM_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5093521.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10832..11176)
                     /locus_tag="ACG8FM_000838"
     CDS             complement(10832..11176)
                     /locus_tag="ACG8FM_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093522.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11196..11474)
                     /locus_tag="ACG8FM_000839"
     CDS             complement(11196..11474)
                     /locus_tag="ACG8FM_000839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093523.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11833..12333)
                     /locus_tag="ACG8FM_000840"
     CDS             complement(11833..12333)
                     /locus_tag="ACG8FM_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093524.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12334..12879)
                     /locus_tag="ACG8FM_000841"
     CDS             complement(12334..12879)
                     /locus_tag="ACG8FM_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093525.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12876..14132)
                     /locus_tag="ACG8FM_000842"
     CDS             complement(12876..14132)
                     /locus_tag="ACG8FM_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5093526.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14493..14804)
                     /locus_tag="ACG8FM_000843"
     CDS             complement(14493..14804)
                     /locus_tag="ACG8FM_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093527.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14827..16302)
                     /locus_tag="ACG8FM_000844"
     CDS             complement(14827..16302)
                     /locus_tag="ACG8FM_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFS5093528.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16751..17203)
                     /locus_tag="ACG8FM_000845"
     CDS             complement(16751..17203)
                     /locus_tag="ACG8FM_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093529.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17205..17570)
                     /locus_tag="ACG8FM_000846"
     CDS             complement(17205..17570)
                     /locus_tag="ACG8FM_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093530.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17669..21952)
                     /locus_tag="ACG8FM_000847"
     CDS             complement(17669..21952)
                     /locus_tag="ACG8FM_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFS5093531.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22099..22338)
                     /locus_tag="ACG8FM_000848"
     CDS             complement(22099..22338)
                     /locus_tag="ACG8FM_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093532.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22428..22670)
                     /locus_tag="ACG8FM_000849"
                     /pseudo
     CDS             complement(22428..22670)
                     /locus_tag="ACG8FM_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22783..23034)
                     /locus_tag="ACG8FM_000850"
     CDS             complement(22783..23034)
                     /locus_tag="ACG8FM_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093533.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(24030..24940)
                     /locus_tag="ACG8FM_000851"
                     /pseudo
     CDS             complement(24030..24940)
                     /locus_tag="ACG8FM_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(25136..26971)
                     /gene="lepA"
                     /locus_tag="ACG8FM_000852"
     CDS             complement(25136..26971)
                     /gene="lepA"
                     /locus_tag="ACG8FM_000852"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS5093534.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            27118..28338
                     /locus_tag="ACG8FM_000853"
     CDS             27118..28338
                     /locus_tag="ACG8FM_000853"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5093535.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28358..28852
                     /locus_tag="ACG8FM_000854"
     CDS             28358..28852
                     /locus_tag="ACG8FM_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5093536.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            28830..29516
                     /locus_tag="ACG8FM_000855"
     CDS             28830..29516
                     /locus_tag="ACG8FM_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093537.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(29667..32276)
                     /gene="clpB"
                     /locus_tag="ACG8FM_000856"
     CDS             complement(29667..32276)
                     /gene="clpB"
                     /locus_tag="ACG8FM_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS5093538.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT         9430 a         6880 c         5009 g        11091 t
ORIGIN      
        1 tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa acttggtaat
       61 ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac ttgttatttt
      121 ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat cgacaaacag
      181 taaatgatat aatccatttc cacctccaag catattcaat actggtgaac atgccttaga
      241 aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt gtttaaaagc
      301 catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc tagcagcagc
      361 tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag cttttcctaa
      421 ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa aagcttttaa
      481 tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc caggaagaga
      541 attaatggct acacatccag catatcgttt ccaatcaact ttctccattc taggaacaat
      601 aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac tgattcgttc
      661 acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa aaccaactgt
      721 atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat caactgtatt
      781 cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta aacctaataa
      841 gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc ctccataaaa
      901 aataagttac tttttaaata tcttgtttat gaattcattc cccaaaggcc ttttaagatt
      961 gatacagcag tccgttcact tcttttagac ttgtcataca agtcttttaa atgatcccat
     1021 atcagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg gttcattttt
     1081 tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc atcttggatt
     1141 tcaataatag tatcatcaat tagtttttga atttcttgcc tatcaacata ttcaatctta
     1201 gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc ttcagacatt
     1261 ttcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg aaccagtaat
     1321 tacactatct acaacgccat tccaatttac tctacatttt ccatttttca gatttgtcgg
     1381 tccatacgga tagccttttt gacattcaat gttcggatta taccagatag gattatacca
     1441 ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa atccaataca
     1501 catcacacta tagccaatta gttttttcat ctcgtatcca tctcctaaaa aatagaatag
     1561 ttcttatagt tattcataag acatattttt tccgtttttg ttttatcctt tctgtcctat
     1621 aacaactttt ttcaagtaaa gaatgacata gtttttctta aaaaaacagt ctgttttttt
     1681 ctatttcttc ggtgtataat aaatatgagt gttcattttt tattttaata cgcactccgt
     1741 acataataaa tcttgatcgc atcaatcttt tgagcttctt gatctacttt caattgaagt
     1801 aatacaaatt gagtaacctt attcattgtt tcttctccaa ataacaaata ttgatccgga
     1861 ttctctatat cttgtgtcac ctggtaaagt ttcccagttg ttttcatttg tacatgcggg
     1921 tctacatcaa tactattgtc tttaatacaa cgatctgtca tatagtccct cacagcttga
     1981 ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt taagaaattt
     2041 tttgtcattt catctatttg catttgttgc gctgtctcgt cttctaatga accaatttga
     2101 ctgttacttt cagtactttg aagagttcca cttgtttctt ttactgcttc gattgtttct
     2161 tctgtcgttc tcaattgtgt tttttgtttt tcagaagaaa accaatgaca accagtaaga
     2221 cctatcaaac ataaccaact aaatactaaa attatccctt ttttcacgtt tctagtcact
     2281 gcctttctta tttcacctta cgaaccagac tacgaatttt tgtttgattg tacgtgcgtt
     2341 gataccgagc cataatccgt ccttgctcgg cattctgttc cacactagag aaatgttgtc
     2401 cgccatatga aacatctgtt ataattccgg tgtgaccata gattccagga gacaatgcac
     2461 cacctgcttg ccaattcacc acatctcctg cttgtaaatc acttggtttt gggttcatga
     2521 ttacttgcca accataggtt gtccagttat aatcttcccc tattaacatc gcaaacatgt
     2581 gtccagaacc cattaactgt gggccaccta attgagatac ataccatgcc gtaccaccat
     2641 aacattcccc attatttacg gatcgtccaa tgatccgatc caaaaccttc gcattgcctt
     2701 tactttttcc tgcaataagc gaagtttttt ttgttggtag gctattccaa tgcttgatcg
     2761 caaaagtatc atattccgtt aagttatagg acgttatgat actcattgct cctcgataat
     2821 acggactccc ttcggtaata cctgctgccg catagcctcc acgacctaga atcaataaac
     2881 aacttccagg atctttattg cccacacatt gattaaagtt gctttggctc gccaccataa
     2941 agccataata gtcataacaa gcttttaagt tcggaaactt catatagaaa ccaccttcag
     3001 aaccaccaat tccacgtgca gaaccttgtg ggtagggaca accaggaaac caggtaatcc
     3061 caaaaaagtt cgtatcgttt cttccagatt gtgactgtcc ataagctgat tcatagccta
     3121 attgagaaag aatcacactc ggtaataact gatactttac agctgcacca actgctgctt
     3181 gcgcatgttc atctgtgatt ggaccacgtc ctttaatctc tgaaaaaatc ggtaatcctt
     3241 cggtatattc cccattaaaa gcgacagcta gacctgtagc ttctcctcca tgctcagaaa
     3301 gttctcctga aaaaaagaat ggcaatagaa aaaagggaag aataaagaaa agaagacaac
     3361 ctagacaccc tttcttcatt tcttcctcat cctttcttcg ataatggttt ttggactgtt
     3421 cgtccttttg gtaaagtaaa atcagttcct ggttttgcgt ccaaaatcgg cagtgtttta
     3481 ggatcctttt ctattttttc cgattgctct gttggcttat ctaaagaaga aaatgcaggt
     3541 tgtaacgatt ttggtgcgga aatcgacggt ttactttcag acgaacgttg ccccggttgt
     3601 tgcacagaac gatttcctga tagagtggct accgacgaat taggaacaga gtattggttc
     3661 aataactgct gcattgctgg ataactccca tttttcgcct gatttttcac ttggcgaatc
     3721 tgttgtgaaa gagaaccagc agctgttaat tgtggcacgg gtacttttcc agaaggcaac
     3781 ggaatcgatt gaccaacaaa agcgatacct ttatttaaca atggttttct ttgattattc
     3841 ccttgttgct tactcaattg tgccaatgat tgtacctgtt gttttggtct tactgaattc
     3901 atggacgaat ttggaatcaa accatttgaa agaccagaat tccactctgt tttcgatttg
     3961 gtagaagaca tttcaggctc taccccatga taacgaacat gacgaccact ataaggcaag
     4021 gcttgatttg aacgcccacc tagttcaaag ggattaacca cttgcctccc ttttaccgat
     4081 tcttgtggtg tacgtttttg ttgaaacttt tgaaatccat gtatcgctgc agaagcgagt
     4141 ggattcgttg ctcgatacac attccctgct aatcctgcac ctttgattgc caaattttgt
     4201 ccagcagttg atagttgacc aggcagttgt ttcatttgag acaaagaatg acttgctatg
     4261 tttttcgtgt tgtttatagt ttgatctgca gctcctaacc ctagtttctt taataaatct
     4321 ttccgtttta caatgagtaa aattgatagg agtgctttga caaacatatt tcctaaaaaa
     4381 ccaaaaacag tattcattgg aattaaatcg tctactatgt tatagatcaa aaatgccacc
     4441 aaaataatca cactgtaaaa agatttttga aacatcaagc ccaaaaagga ctttaaggta
     4501 ttcatgagca tataatcaaa tactggaaca aaagaagcga gtaatgaaaa aggaatcaca
     4561 agtaataaac acaacaccaa catttgaaag ataaaacgaa tcatgccaat cactacaagt
     4621 ggtaatccaa tacttagatt aagcaatggc accatagaac caacgactgc tttataaaca
     4681 gctttagttg gttgaatata gacacgataa gccttctttt tccctctatc ttctcctgca
     4741 gcagtggtca cttttgtctc aatatcagat aattcttcac ttgacgcttc cttggctaga
     4801 aattcttgtg gttcaatctc tgcttgatac aattcttcta aatttgttgt cccataattt
     4861 aacaatatat acggatcaac aacagccgct ttataataaa gttggcgcac tttttgaatg
     4921 gcttcctttt gtgtcgttgg cactaaaaca ccattgacat cttttgtcgc caattcatct
     4981 agactatttg tagcagaaaa aataaagcct tctacctcca cggacaaatc atttaaccct
     5041 ttggtaaaag cttctccttt actaggtacg ttactaccat agccaaacca aaccattgaa
     5101 aaaatgacaa cgaagagaaa tcgaataaac accatttttg cgtaatgttt ccctctaaat
     5161 atacgaagag taaatagata taacacatac aagacgacaa tccccgcacc aataggatcg
     5221 tatatttttg aataaatagt atccgaaaaa gtgaaaaact ggtgaattaa ctcgttcatt
     5281 gcagctcctt catagagctt ctcaatgaca aaatcaaggc cttgccaaat caatttactt
     5341 aaactaaaga acatattcgt catcattttt agaaaatctg tgccatttgc cccaaataac
     5401 attttgccta atgaaaattc actatcagat aaataagaag aaaaggtatc aatatgaaat
     5461 ttcccaatct catagatcga ttgatccacc tgcttttctg ccgcatacac caatccacca
     5521 acaaaaagca gtccgaatcc aatcgttaaa aaaaacaaaa cgcaccattt cttttcctta
     5581 tgctttatat aagccattgt ttcacctcat ttctattgca tgattaacgt atcctgttct
     5641 tctacttgag agtgacttgt tttttccgtt gtttgtaatg cagcggtcat ttcttcaaac
     5701 aaactgtgta cattgagttt tcctacacgt ccatagggat ctaacatcca acactgtcct
     5761 ttaatcatgt ggctcatttc agctaagttc gcttcattta cttccaaacg taaatgattc
     5821 aaaatctttt ctcgctcact gtcttcatca aaagcaaaga cgacaccaaa attccctttt
     5881 tctttatctg aacctaagtc accaataaat tgtgtatcta acactaattg attcatctgg
     5941 gaacgcccca cacgtgccat tgctgcaata atactacgtc ctgcgttcga attattaaat
     6001 acccatgctt ctgtaaaata aatcgctgtt ttcttactac tatctcgttt tccaaacatt
     6061 tcacaaaaac gacctaaact aatcatcaaa cacaaagact ttcgatccgc atctgtatat
     6121 agttctggtc ggacattgtc atttggtaat ttcaaatctt ggatctctaa aatcgttgtt
     6181 ttggcattaa agtctaatcc cgggttttct ccataagaaa atcctaaacg taaaatcgaa
     6241 tcttctaccg tcaagcgtaa aaactcacca tattctcgaa cctctttttg ttccatttgt
     6301 tctaaatagt cgaccacatg catcaagcca acttgttgtc cggcttcttt ttgaactaaa
     6361 acttcttgga ttccctttaa taccgcccct tttaaaaggt gattggtacc taacggactt
     6421 aactcatcaa tcatatcctg ggctacttgt ttcgcatcta ctcgggataa acaaacaatt
     6481 ggatctaata caccataatt acggggattt gtagcatcta acgtgacata actcattttt
     6541 tgcaaatgtt ctacaaacaa agggtaatgt ttctgataat acgggtcttg aatgagtttc
     6601 gtaaaccatt tgcgcttttc tcctttggga tccacaaata aactttgtac attcataaac
     6661 gaactataca aaaagagcaa gccaactaaa aaggtcttcc ctttaccggt ttttccagtt
     6721 actgctacat gaggcgcatc aaacaacgca cctttgattc cttccgcaat ggctagaaga
     6781 ttcaaaaaga caaaacgatt tgacgcataa attaattctt ctaatggtgt tttttctcct
     6841 cgagaaccac catcccctaa tgtatcttgc cgaccaatat accagccgga ttccatacct
     6901 aattctgata ctgctgaaaa caaccattcg ccaactgctt ctacagttgt tcggtgcttc
     6961 caatgtaact gccgtcccaa tggttgccct aataaacatt tatagaacaa ttctcgttga
     7021 tcattttttg cacggtggat atggataatc gtatgaagtt gttgaataac cgttgcacta
     7081 ttttgacgaa cttcttcaac cgtttgaccg tatacaataa aacatgcgat ccaatcgacg
     7141 acaggttgcc cattatctac tcgttgcttt aaatgtttta ctaatcctcg aacagttaat
     7201 aagcgttcac taggttcttc atcattttga accaattcct tatgctctgt tttgaaccgt
     7261 tccgatagcc aattcagttt taaaccaata cctccctgtc ctttcaagcg tggatagtga
     7321 attttcattc gcaattctgc tgggaaagaa agatcctgag caaattgaaa tagatcacaa
     7381 tttctcatat tttcaggtgt ttccccaata ggcaacaatg ctaaataact actccctaac
     7441 tcatcttcta attgaagaag gcctttatgt tccttcggta tacgtacata cgttccttga
     7501 ttgtctttca gtacttccca attattttgt tcttctactt tgtgagccat tcctcgaata
     7561 tattgcagac gattgagata caacagctct ttttttacta agggacgaat agaaaagcta
     7621 taaagtaatt gtgtcacttc ttttacataa ggatcacttt cagaaaacaa agtttccaaa
     7681 tcatctactt ctaatccaaa taagcgtgct aaacttcctg ctaaatctaa ccagattaga
     7741 tttattacat catgaaaact ctccaccgtt gctttttctt tgactttgat tcctaaaata
     7801 aatgaaggac gatagacttg accttctcgt tccaacaatg tcactgtttc ttttgcataa
     7861 tacgcaccaa ttgcttgact atcgggagaa aatccctttc ctaatgtgtc aaacttcgtc
     7921 cgtaaattca ttgttcgtgg atacatcacc aactccacat cttcataatc tgctaaaagc
     7981 aacagtaaac gagccatcat ttcttttatt tcctgtgtct tttttggtgt gttttttgaa
     8041 attgttcgtg gaaaaacatg atagtaggcc cagatacaat cctctccatt taacaaacaa
     8101 tttttcttga atgcttttac tggttctttt acaagcacca atgaatttca cctcccaata
     8161 aaaaaggaac aaccagttga ttgtcccttt ttctaatcct ctaaataaac atacttattc
     8221 acaaaaagta attcttccaa tgcattgttt gataccttgt cccagttata atacacacaa
     8281 tcaccattca tcatacgaat catataacaa aaccaatccc ataaataaaa atgcactttt
     8341 tttccatcag gttttagttt tgccagtgct aacacacata aataggggag tcctatataa
     8401 aaaacaactc ctgctggtgg aaaaaaagaa atgacccaac caataaatga acgtaaaaat
     8461 aaatattcaa agattacgac caacaaaaaa gttgctgcat tgactaatgc aactggaaat
     8521 ggaagacgcc aattgcttgt aatctgttgt acgcgataag gttccgcata aacaatcgaa
     8581 taatcataag gttcccaatc cacttcacta tcctcctaat ccataaataa tttttcaatg
     8641 attttaccaa acgcatcaaa tacacgtgtt ggatcattta agaagaaata aactgcggca
     8701 cctgctgcga tagatattaa tgcatttcct acttttgatt tcataaaatt tcgcaatccc
     8761 agataaacaa ccaccattat taaaaaatag ccaccttgcg ttaatataaa atctacaatt
     8821 cccttcagtg tcatttccgt tcttctcctt tcctatccaa ccaattatgt gttaattttt
     8881 caacataata ctgatttgtt tttttcgtaa tgacaatcgt catttgttct ttttgtaaaa
     8941 gtgacgtatc accatctcga aagacgaccg ttgcttttac aataaaaggt tttttcgttt
     9001 ctttataacg caatactttc atctctactt gatcaacttt agctgtttga ttgagtcctt
     9061 caggttcttt catcatgtat gacatatctt ttgcattgtt tttcgcatac ttatctaaga
     9121 aatcttccac aaatccttca acatctttcg cttccgacgc atcaaccgtt tcgtataatg
     9181 tgtcatcata agtaatcatt cgttgttttt ctgtattctt tggtagtgcc gtaaaataag
     9241 gataagcaat aatacgacag cccttgtttg aaatagaaaa gggaagattg agtacttgtg
     9301 tgattgtcac acttgtttct ttcttactct ctggattcat cgagcgaata tcataagtta
     9361 ctttataact agcagttgga atggtttcaa tttcttttaa tccaataaac gaagcagcag
     9421 ttaattttcg aacacctgaa acacttcctg gttcttgaat aaagttttgt gcataaaact
     9481 ctttttttaa tgtttcttga cgttgttgtt cttgttcatg attatcgaat gtattcatat
     9541 acaaagcaac aaaacgattc ataaaggatt caatttcagg tgtgtacgtt aattctgacc
     9601 gactttgtac ttctttcttt tgtaattgtt ggatttcatc tttcaaaaac gacacttgaa
     9661 ccttcgtttt catggattga aaaaatgcca agccccctga aaaacaaaga aataaaacca
     9721 aacaccaaat ccatttcact gctttttttg aatggatttt cttttgcttc ggtactttct
     9781 ctgtataaac tttttctttc cgccgtagtt gtcgtttttt cataagctac tccttctagt
     9841 atgtttttac tagtacgtac gattgacaac gataagcttg tttttctgaa aaatagtcca
     9901 ttcaattacc ctcattttct attccttttc tctttctttt atatccaaca aatccagcaa
     9961 tacttgtcaa cactcctcct agaacagaaa gtgcaacatg tatttcttca ccagtctttg
    10021 gtaagacttt tgtttttcca tctgctgttt tgatttcaat attgccactt ggaagtttct
    10081 ttacaggaat cgacaaatcc tttaccaaat tcccttcact atcttggatt aatggaatac
    10141 catcttttac accaacaatt gtttgatcac ctgctacttt taatggggtg gttggtgttg
    10201 gaatatgggt caattcctct acattactag tgacaatcgg tacactcatt ccttgagaag
    10261 tattcgtcgt aatttcaccg cttggggtta ccgatactgt gacttctttt gctggaacaa
    10321 taggtttatt ttcttttgga attgcttctg atttttcaac aggttgtttc ggttcttctt
    10381 tttgttcttc actggtagac gacatcgttg gctgttccgt acttgacgaa tcagtattct
    10441 tttcagaaga actggttgtt cctgattgtt ccggagttgt gactatactt gaatccgttg
    10501 ttgttgaact atgcacacct tcttcttcga tcgaatcagt cggtaaagtt gattcaggaa
    10561 actctggttc tggtgtcact ggctcttttt caggtttctc tgactgcaat tgttcaggtt
    10621 ctgctggttt ttccgttgtt cctccattta gctctgtctc aacatctggt aatccttccc
    10681 ctggcaatgt ttctacaatt tctgacttag gtgtttctac agtttcatct gcataaacca
    10741 atgaactccc taattgaaac gaaaataatg aaattcctag tgttgttacg actgctaatg
    10801 ttgttttttt catgattatt cctccaattt tttatgcaat tcgtgttttg ttcatagacg
    10861 ccgcactaat attatctgaa cgataaaaga actcccgtcc gttttcttct aacccaacat
    10921 atggattatt tttgaataat tgaatcaata gttcactggt gttgtatttt ggatagtttg
    10981 caaaaaaata atactgtgct tgtcgcccat ccgaaaattc aacaagtaat tcataacgat
    11041 accgcttcaa tccttttcgt tgttttaaag gatctctata ccaacttgtc catcgttcca
    11101 ttcgtggata gagatagaca cgatacacat agaacaatgc cataccacat accaccaaaa
    11161 ataataatcg ccacataaaa attccccctt ttcttctacc tttcttgaat catcgaacat
    11221 tcataccctc tatagagatt tgaaaaatcc atcatcaatt gttcatataa acgttgttgc
    11281 aaatagcgat tgtttcgaat acataattct tcatagccat caatcacttg atcttctttt
    11341 tcatagcatc ttgtagctct atgacgctca gcttgcaaga cataaccaaa ctctgcacca
    11401 tatcgttcta ctgttaataa ctgttgacta cgcaataatg caatataaca atcacaaaaa
    11461 tgttcaaccg acatttgatt tttctcacgt ttgtgaaata ggtattcttt agaaagcaca
    11521 ttattcgtta aacaatctga taaatagcga taatctggcg gtcggttatt ttcatctgtg
    11581 aatttttccc ataataaact gatttttatt cgttcagcgg ctagatagtc ttgttcgcgt
    11641 ggataaaatt catcaatcgc acaaagtttt ttatcacctg aataaacaaa tccatataat
    11701 gcgaaacgaa ctcttccttt tcaattggct ctcctaaaaa ttccagtgac ataccatctt
    11761 ctccttccta aaaaaaataa aaaagcagaa accgatttgg cttctacttt tctttccttt
    11821 tatagctctt ttttacggaa gaatccgcaa atgacatttt ttcaaaaaat caatcggcac
    11881 aagttcacga aaacttggag caattttgga tagaatcact tccccatctt gtaaaataca
    11941 atcataaaaa tcatcagtta cttgatactt acgcttctta caataattga taaaatagta
    12001 gcgatcattt tctatttcca ttcgttgttc tactaagcgc atacgaacat catcatagaa
    12061 ataagccaca aatcgccctt caagttcatg tttttcaatg ggatactctt ctggtaacaa
    12121 tctatttttt cgtcgtttat acattgctgg ataccttaga agaagttcat ctaaacgtct
    12181 gtcagtatat ggtaactgac gaatcaacga ttctaatcgt tctttataca gatagaataa
    12241 ttggcgcaag caatagtgta ccgcctctgg attttctaat actgttggat cacgtgacca
    12301 atacgcacca acataaatca taattttttt catttagaca atcacaccag caatactaat
    12361 gaccgatcca caataggtag aacaagtggc atagcgacca atttcctcta attctatctc
    12421 aatctcttct aaagaaagat atgtctgttc ttctgttggc tggcgactca tttcccaagt
    12481 gacagtcacc aatgaaattt cttccataag aggtttttcg ctgctaagtg ctgccacaca
    12541 gttttcatat atcttcattt tatcgcagcc ttttttaacc gctaaaatcg caggtattgg
    12601 caatgaaaat cgataaaagc gaatgaattc tagccttcct ttccttcggc taaattccaa
    12661 ctcttcgagt tcttgttcgt atcccttacg aacttctaat aagcgtgcaa tttcttgcca
    12721 gttctctttt tcctgataac gtccagcttt taccgcttgt tcaatataag gaactcgaaa
    12781 acgaaaataa gacaaatcaa ccatcttttc atttaatgct tcgattgcag cctttaaatc
    12841 caacaggcgc agccattctg ttgtcgtata tgcattcatg caattttccc tcctgacgtt
    12901 cccatccatt ctagatagtt ttgttgctcg tcatttagtt cggcattatc aatcccttct
    12961 tgtacaaagt ctcttcctaa aatacgatct gcttcttgta acaactttaa tgtcggagct
    13021 acttgtttca aaaaccagcg ttctgaacga tctaatgaga atttttctgg ctttactgta
    13081 aaatccaatt cttccatcga acgaaataat tttgcccatc gtttatcaac aatccgtgca
    13141 tcactttcca gtctatccca aacaataagt tttgcattaa ttaaaccaac gccaatctct
    13201 cctaaacgtt tcggtgaatc acctaaaata aatgcttcta ctgcttgtgt tgctttctca
    13261 ttggctaacc gtaactcata tcgattataa ataccatatt tcgttaatgc ttctaattct
    13321 gaaatttttt ctcggttcgc aatttcaaaa cgtttctcat aaaaattaaa aaagagagga
    13381 ctctttcgcg atccaaaata caaagataat ccttcacttc gaaaatagcc atcatctaaa
    13441 aaacaaccgc caatattctg gaatctctta aagtccattt ctaataaacc tgctttttgt
    13501 ttttctagta attcaaagag atcgaaatgt tcttccgttt ccaatcccca aagttcatct
    13561 aacgcaatat cgaatcggct acattgcaca cgtgtataac cagcttccaa ctttccttga
    13621 tttttccaaa tggaatatag ccatgttcgc caatctgttt gttgttcacg catgacttcc
    13681 tcatattccc ggcaaccttg tcctgaaaat tgcacaagta cattttctgt tacagttcgt
    13741 tttgaatgcc agaacaccca gatattccca cgaacagaaa gacagtcaaa aagatacaag
    13801 cccgttggac gtgtttcaaa ttctgtcaga tccattcgta atacacgttg aatcactgtt
    13861 ttgtagtctg tcccaaaaaa ggaaagacgc aaataatcaa tcattgctcg aagatctatt
    13921 ccgcttttca tatacaatcg aaagcgttcc ttgattcgtt gttcaaaatc ctctgggatt
    13981 tttcttttcc cttgttccat ctttctaatg taatcttttg agcagcccat caattgcgca
    14041 aatcttgctt gtgatagcgg ctttttatct ccacttatat tatgaattgc acttaacttc
    14101 tttctgtagc tttttagctc aatcggtgtc attttctcgc tccttttata aaataacaaa
    14161 atcgattttt tttaaccctt tagaaacacc gatatatcaa ctagtccccc tcgttttttg
    14221 ggaaaagtag tacaactgct gaccttagat aatcacttga tacaaaaggg tttataaaac
    14281 ttttttctat gtgttatgtt atttctgccc cccatattat atatcggggg gctttacaca
    14341 aagaggccgt gcgcaagcgc acgacctctt tgtgagctgc acacgctgct agcgagtgcg
    14401 cattataggc ccatgcttcg cagccttaat gcgcaccgcc aggcagagtg cggctaccag
    14461 actacgtcta gagccctccc gatagataat aactacaaaa ttaaatgatc taattttgtt
    14521 atttcactat atttttcagg agaaaaaacg cgataaattg acaaagttaa atcctcttct
    14581 aaatctactc gataatgata caatcgagtc aatcgttcta tatctttttt cgttagcaat
    14641 acttgattaa taacttttcc tgtaaaaaca cgttctttgc gcaaaaagcg gtaatcagtt
    14701 acttgttcaa tacgattcaa ccaatcataa aacgttctgg aagaaagaga aggattaatc
    14761 attcgtattg ctgtagctac tctataataa caactctctg ccatttaact tcacctcctt
    14821 agttgattac aaaatatgcg catcagaaaa acttgttcct tttggatctt ttgctaaata
    14881 catttggcgc tctagttgtt cttgtaacaa ctgattatca tctcccatcg tttgatcttg
    14941 actatcctct tttactgtaa aatcatagac aatcatttca gtaaatttct caaattcttt
    15001 gataaagtga taatctttgt aaacaaatgg cgcaaaaaac tctacaggta catcaaatcc
    15061 tggaaaagca tatccacgac caggttgatc tttgttgtaa aatggcttat ttttaaaatc
    15121 ttctccaaaa gtcattaaat aaccaatggg agacaatcga ccaacagaaa tacggaatgt
    15181 taaattatcc cgaacaccac ttggaaaata ttctgcatct ggtcgttgca ttgctaaaat
    15241 aatgtacatc cctgcttgac gaccctttaa tactaatggc tgaatcaatt tatcgactct
    15301 atttctcgct tccatatcca acgaaccata aaaagctgcc cattcatcaa aaaagataaa
    15361 atgcggtggt aacccataat aagcataatt tttccctggt tcatatttag gatctttcat
    15421 ggttttcata taggcatacc gttgattcat ttcttttaat gcatttcgta aacaaataat
    15481 catttcttca tttattttgt aaaacacatg tcctttgaat acatcgacgt cacctaatgc
    15541 tgctaaatct gccgcttttc catcgcaaat atctactgtc ccaacactta aacaagcttg
    15601 aattaaagaa tacaataaat aggttttccc accaccgatt gcaccagcaa tcaacatatt
    15661 tggtgattga tcatataccc aatccaaagt tttcattaaa tgaatacgtc cattttctgc
    15721 tatacattga tcaataggaa tccgaccaga atctgaaagg aatacataat tgattcgggc
    15781 tttttctcgt tcttcacgaa ttaaatcacg caaaaatact tcagataaca aatgacccat
    15841 ttttaaaaat cgttcgtgaa aacgcccacc atctaaagga aaagaaacca ctaattcttg
    15901 tttcttttga taaaaataaa cttttggtaa tctcattttt tcccttgttt gtttctcact
    15961 aacttgtttc tttgtataaa caaaccgatt gtcaattaac attcgaacaa gtaactgacg
    16021 ccaataaaca gaggcaaaat atccttcttt tagtcgcgac caatgcatca accctaaaat
    16081 taacccacta accagcaaca aatacaacac aatacttatt ccaggaataa gatacatttg
    16141 ccaattcgaa aacgtactta aacgtggata aaatactttc caactaaacc aacctaacag
    16201 aatcaaaaat ggaaacaaaa agaaaccata atagcaaaac cataaataac gattgaaata
    16261 gcgaattcgc cgaccacgat acttgaaaaa atgagctttc aattgtcaca ttcctccttt
    16321 ctgtttttac ttttcctgtg ctaaaaactt ttgacaaaaa gcaactaata caggaatatc
    16381 acatttagta aaaaaacata atcgaaaaag atttaatcga tttttataag ggtgtgcaca
    16441 catattacat attcgtacta tacgaatcca aatcacttcc tgatgatctt gtttccactg
    16501 gtacggtaat gaaaaacgaa caagatcacg taaaaacata cactgcccaa taaccgcaca
    16561 aatatatgca tttgtttgta caaaaatcgt tggattgtct ccaaaatact caatcaattg
    16621 ttggatattt tgataacaag cacaatctct caaagtattt gattggtttt atctgaattc
    16681 aaaatctccc cttccttcta tcttagataa aaagaatagg aagactcctt cctattcttt
    16741 caaaaaaacc ttattttttc tctttttcct tagaatcaac acttggttgt ttttgatcta
    16801 aacctttttc ttgtgattgt ttcatattag gattatttgt tggagcttct actttctcta
    16861 atcctttcgc ataaatcact aaatctgaat attcacgatt gccatcttgt gctggaatat
    16921 ataccacatg atattctgga tcaattaatt tcacaggatc acgtcgttta aaaccagtca
    16981 aatctacatc tgctggaaat ttaactcgtc gtgcaatctt tggttcatct ggatttccaa
    17041 aaactaattg tgctcgctgt gatcgaattt ctcccgtata atcgcctgtt tcattatcaa
    17101 atacacgatc agactcttct aatgcgccaa agcgtacggc tcctaaacaa gtttcatcta
    17161 atgcttctgt aatgtcataa ttatttggaa attttaatcc catcttacaa aacctcccca
    17221 atttttacac cagttaaaaa gacaccaaca tcttgatagc cagttgttcg acgctctgtt
    17281 tgtcccatat catttgtttc acgactattc actcgttctt tacgaaaaac aaaataagct
    17341 tcttcaaatg aaatacgcac ctctttttct ggtaatcgta atcttttatc ctcttcagct
    17401 gtgagataaa caaaaaaagc aggctgttct tctcgcgaac actctgcttg taattgaaca
    17461 tatcgcccag acacaccatt aatttccttt ggtggattag gttcccgtga acccaataaa
    17521 aatacttgac ctaaaaactc tttcttcata cgcaatgctt tttctgccat aaagtttttc
    17581 ctcctcttaa aaaccaaaat aaattctaaa tatttctaca ttacaatcaa acaagcggtt
    17641 aaaaatccta accgcttgtt cctcatgact aatctttggc tttacgtact ttataaagtg
    17701 ttatacctaa agcaatgact aatactacta atccaagaac cataagtata ctacttgttt
    17761 gttcacctgt ttttggcaac attggtacaa cagtagctgg tttaacccgt tgttctggtt
    17821 tcactggttg cggtggtttt ggcgcttcca caatttgaac cgtttgatct ttgtttgtta
    17881 gatctacatt gtgtttcgcc acttcttcaa acgtttcttt ctcatcttcc gttttcgctt
    17941 ggagccattc cgcaaaactc aagccttttc ctgcaagatt gcgaccgtcc actaagaatt
    18001 caaataagaa ttccatcatt tctttatcgg ccgcaaagac tttttccgca tgagtgcctt
    18061 caaatggtgt gccgtctggt gttaagtagc cttgaacaat taagcgatag atttgaccaa
    18121 tcgcaagacc gtctactttg acccattcaa gcacagtttg atttggcaag gccaagatct
    18181 cttttccgcc atttttattc gtcgctttcg tcgtaatggt tggattactg attttgatgg
    18241 tttggtcatt gtcggtaata tctgcgtgag aagccaataa tacgccttct ctaaacagat
    18301 cttcaaagac cacgacttct ttgcctcgca acgcacgtgc gtctagaata aagtctagat
    18361 tgactgtacc atccgttgtt tctgccacaa acgttaaggt acttgtgact tcttttccat
    18421 ctactagcac tggtttttcc gttactttgt ccattagaac accgttgact gtgtatgttt
    18481 ttccaacgac caaatcgtgg tatttcacag cgtctgtaat ggtgactttc gttaatggat
    18541 taacctcttt caagccagtg ggtttatgtg tagccgttgt acgtacactt gggttagtta
    18601 ctcgtaccgt ttgccctttg tcgttgatgt ctttatggtt tgctagttct tcatcatttc
    18661 taaaaagact ttcaaacacg acgatttcac gaccatttat acctgtcaaa tcaaaggtaa
    18721 atggtacgat caccgtttca ttttctgctt tggcaataaa gccaacactc gtttctaccg
    18781 tatttccatg cacaagcaag ggttcaccgg ttgctttatc cattaagatc cctttgacca
    18841 agtatgcttt tcctggaaga atctcggtaa tttcaacggt atcgtttaag gtaaacaatc
    18901 ctgttgggtc tgcgactgtc aatccgtctt caaagacagc cgtggtatga acggttggat
    18961 tattcacttt cacactttgg gcttcatcgt tgatatccgc atgtgctgca acttcaatgc
    19021 catcacggaa aagtttttca aaaacaacga tcgtttgacc tttcagaaca ctagcattaa
    19081 ccacaaattc aaggtctttt gtgccattga ctttgtctgc aataaaggcc aaggtacttg
    19141 tgatttcttt gccatcttgt aaaattggcg cattcgttgc cttgttcatc aatactcctg
    19201 aaatggtgta ttcttttccg acaaacaagt tggtataaga aacactgtct ttaatgacga
    19261 tcgtttctaa tggatcaacc actttttcac catcggcttt atttgacgct tgtgtttcta
    19321 gttttggtgg aatcaagtaa ttacgtacag aaaccactaa cgtttcccca tcatctggcg
    19381 cattttgttt gaatgcaacc gtgaatgctt gtttatctgt atttggttgg aaactttcta
    19441 atggggcatt ttccaagaag taatagtcat cggcaactag acctttcact aaaatttccc
    19501 cgttttgatc agtggtatat tcaccaattg cttgattttt accgttatac aaggtaaagt
    19561 tcacacctgc gagcggcgtt ttcttcttgt tccattcttc atacttgaat aattttacac
    19621 ttttccgtgc ttgacggttc gcaaaatgga tcactttgat cggttctgta tcattggtaa
    19681 tcgagaacac taatgggttt tgattcaaaa cgtaatttac gggggctttg atttcttcca
    19741 aacgatagcg atcttttcca taagtaagtg taccgttagt taggaaaaag cctttttcat
    19801 tggttttaaa gatatccgtt gtttctgtat catttggaat ctccattgtg acataacgat
    19861 tggctaaagt atcaaaaatc ttgaattctg tattagcaag tggaatcact tttcctgttt
    19921 cttgatccac tttcaccact ttaagacgtt gttcaattac tttatcttct aaaacataat
    19981 ggaaagtttg cccattttca gaaattgtga cttcaagtgg ttcaaatagt ttataccctt
    20041 cttttccttt ggtttcagaa agaatgtacg tatcaaagac aacggtattt ttaccaaaag
    20101 tagcataacc atcattgtct gtcacaacgg ttttcaccac tttgccagtt gtttttgacg
    20161 tcaaagtgat ttcaatgcca gctaattttg gtttgttatc tggattcgtt gaatcgacta
    20221 aatctttatt agcaactttc actaaatcaa tggttccttt gatcacttga tcttttgcgg
    20281 tcgcattgac agtttttcca ggttcaacaa caatttcaat tgcttcatca gaaagcacat
    20341 aaccttttgg cgctttgatt tcttttaaaa catatttgcc aggatcaaga tctttaattt
    20401 ctgcctgtac tttgccgtta atttttttca aggtaatttc tttaatcttt ttcccatctg
    20461 cttgatacaa accatagacc gcattttcta aagaagctgc accttgtgct ttcacaccag
    20521 tttccgcatc ttctttaaac acacgaatgt ttccttgttt tagaatttgt aaattaataa
    20581 cagacgcatt aggttctgtt ggatgtaaca caccaattgt ttgagctcct ggacgacgat
    20641 aggctaatgg cgcaccttga actttaattt tattcaattg aatttttgct ttatccggtg
    20701 cctcttttgt gactgtaatc catacttcat tgccgtgaat agatacattg acaccttttg
    20761 gtttttgaat gtctttcaca tatttcaaaa aatcattttt attctttact ttcaacgttt
    20821 cgccaatttt taccgtatgt gtttgattat taaaagttgc tttttcacca aagttattaa
    20881 tcaaacgttg aatttctgct tttcgacgat cataatcaac acggatattt ttgattggga
    20941 tttttgtgcc caaagcttcc caaatcataa attgtgccgc aatgtattgt tcatcggatt
    21001 tgtctttatt tgtgacatat cctaaataag agatcaagct tgtttttttc cgtacatttg
    21061 aatttttcaa atagtttttc aaagcagtag ctgtataaac ttgaccttct gcggctgaga
    21121 caccaggctc aatacaaaac gccacttgtc cattaatctc aaaacgaacc atataattta
    21181 aagtgaagtt tagattgttc ggactagaag taacaccaat attttcgccc caatcaattt
    21241 tccgaccacc taaaacagct gcattggttg cacggcgaac acgaaccgaa cgattggttg
    21301 gtactgttac taacagtgta ggtgtacctg tttttggaat gttctgctct tttttctttt
    21361 gattttcaat actagccgta gattctttgt ttggtttcgt tggttgattt tgactagctg
    21421 cggttgaaga gattgttttt tcaacgtttt cctcattttt tccttctttg tcagcagtag
    21481 ccgctggttg ttccactgtt tcttctattc gtttcttttc ttccgcttgt ttctcagaag
    21541 actctggctg cacagaaggc gttgcttcgg cagctttttc tccttctaga gtggacgttt
    21601 ctgaagcagg ggttgtaatt gaggcagttt cttcaactgg ttgtgaagta ccaggactat
    21661 tccctgggtg ttgtgtttcc ccaatcgtct gatcgagagt cgttttggaa ttgaccgttg
    21721 gcgcttctgt cacaggggcc tgttcaacag tggcttcacc agctgggacc ccggcttctg
    21781 ctggttggac ttctgtcact tcagaagcga cttgggcaat ggcttccatc gtaggttgta
    21841 tagccgtcgt ttcttctgca tgtgcttgag cagttcccac caagaaacag aattgagaaa
    21901 aagccaccaa tgcgagtgct ttcaaccaat tcattttcgt ttttgcatac acaagaattc
    21961 ctccttttcc tgagaatttt atataataaa acaaaacgcc gttaccaaaa gtgataacga
    22021 cgtgaactgt ctcttctatt tttatctggc gctcttatct gaacacattt catcaatgat
    22081 tttaccctcc cactataatt acagaacaaa aaaagaacct gcttcgtgga aatcaactgt
    22141 ccatttccca attggaatac cagctttttt ggcaacttca ccatcaccaa atctcatgcc
    22201 ataccaatta ctaccttcat ccttaatttc tttccaacca tatgacatag cttcatctaa
    22261 tgaatcaaaa atcatgacac tattgccaat tgttacagga tctggcttaa gatcttctgg
    22321 aagagaaata tcaatcatac cataatgatc cgattcctca gtattattgt cattactagc
    22381 tactggtttg gttgttctac tggcttattt tgatacaaca agtactctta ttcttctttt
    22441 acttctccgt tttcaatttc ccattcaaac cactccgcaa agtcttgctt aaaaccatct
    22501 actcgatacc tattttgtaa ataggcatct tctgacgtcg taggatctaa ataggtaggt
    22561 ttattctctg gacgagcata gacacctacc catgtttcgt aatctccttc aaaataacta
    22621 tctaccaaat agcctttttt tctaagaatc tcccgaactt gatttttaat tattgatact
    22681 tgttcaaaac catcactcat tctatcacct caaataactc tagattctca taaatattcc
    22741 cgacaatttc aatcctatca atgatctcta acaaatcgct acttatgctt ccccattcgt
    22801 ataagaattt cccttccgca aaataaattt tccataaact tcttcatgat cgtcccaacc
    22861 aatatcactc tcgaaaaatt ccacgttgtt cttatctttt agctcagtaa atgccataag
    22921 ttcaacatgt tttccaacct cgaaacaatc acaccaccca tcaaccgtta cacgtgtgcc
    22981 ttcaatacct aagaaacttt gtcctcgtgt attaaacact aaaactttcc gcattttttg
    23041 ctcattaatt aatcacgcgc gaaatctagg aatcacaact tattcgcttc ttctttataa
    23101 ataaaaaaac tccaataatc ggaaattact atcttttcct cattcctttt ttaaatcttc
    23161 atacttcttc ttatctttgt cctaacctaa taacacaaat tttgaagaaa aactcagtaa
    23221 aacctgattc tttttttatc ttcacaacaa ccaacttttt cttcagatcc ttttactaga
    23281 acttgtatac aaaaattgtt gaatgctgat ataatttgcg gctacttgaa aaaacgatag
    23341 atgtaaaaag ataaatagaa tcatttttta ttctatatca gtctatgatt cattgcttat
    23401 caatcaccca acccattcga tgccgttttt tttagagaaa tttaaaaaag accgtacgaa
    23461 tctcgcatta tccgattact tttccagaaa agatccccaa ttctgtataa ataaccctat
    23521 cttcttgttg gtttcatttt ctcatataag aattcagtca atctacccac tatgaccatt
    23581 actacgtcca acacaactga catattaacc acgtaagtat tccctcacag atctccgaat
    23641 ctccatggta tggattccca tcctgacgct gcagaggaca acatatttca tgtggctctg
    23701 tacccttttg atttggaaac actacaaatc aatactcatc tataacttat caagcgaact
    23761 ttcgctaggc ttctagtgat tactaaattg ttggataaca tacatagccc attttatcta
    23821 gctgttattc ataacagtta gttcttgtat tacttcttcc gcttgtgttg ccattgagct
    23881 tgtcaaaaag ttacttagaa aaaagctgcc agtaaagcaa ctttcaacaa accctttttg
    23941 tttcttatca tatcaagaaa cctcctttta taaatttttt ctataataaa aaacgtcgat
    24001 aacttaataa gtatcgacgt aaactatttt tatatcccgt aaatcaaaaa ttctaaagaa
    24061 agatttccta tataagcata ttggataatt cgattagtat tgggtatatc tctaccattc
    24121 tcatacctat ctatggacca ttttgaaatt gaaggagaaa aaagctttcc aaactcctcc
    24181 ctttctagat aataatcttt cctaattttc ctcatcctta atcccatact aaaagaattt
    24241 atctttttaa aatcgctaac tctttcatct tttggaagta tattcttata atcttctccg
    24301 tacataaggt aaggaacagt gaccttacca agataggcaa tattcattag tttttcaata
    24361 tctggaatat taataccttt ttcccatcta cttataacat ttttgcctgt atagatggta
    24421 aagtttttac caaatttttc aagcgtctct tcccggttct ttcggacttc tcgtattctt
    24481 tttcccataa caattttatc gtcttttgat aaactaaata tctcactatc acgattaatc
    24541 tccttcaaaa tttctttttc attacttaat agaaaaatat cgattttttt tgcatttctt
    24601 ttgacggatt tctctttcta tttaacacat aactgagtaa tacttcacta attcctatat
    24661 tatcagctaa ctctttgtta agtaacttat tcatatctct gtatgcaatt agtttaccga
    24721 tgagaatctc aatcttatct tcctcatctt ttattagagt ttctatattc agaatcttgc
    24781 ttagagtatt atctacgtct ttagggattt ttcttgaccc tttttcatat ttaactaaag
    24841 tactttgtga aatacctgct aaatcagcta actttttttg agatagtttt ttctcttttc
    24901 ttagagattt aagaaccctt ccacttgaat tatcaaccat gaaaaaagca cctcctatga
    24961 gatgctttca tcatatactg acagctaaaa atcttcaaga aaaatattcc aattttgtta
    25021 attttagtat cgccgtgata accaaatgac agtttaaaat cttttataca gataatcctc
    25081 agtgttgcat ctactcaaaa aggtttattt aataggtttt tcgattgatt ataagttatt
    25141 tcacactatc ttgctcatcc attttcagaa cggccatgaa ggcttcttgc ggaacttcga
    25201 cggagccgat ttgtttcatc cgcttcttcc cttctttttg tttctctaag agtttacgtt
    25261 tacgagaaac atctccacca taacatttag ccaagacgtt tttacgcaag gctttgatgt
    25321 ctgaacgggc aacgattttt tgtccaatag ctgcttggat tggcacctca aattgttggc
    25381 gtgggattag ttttttcagt ttttcaacga ttgcttttcc acgttcatag gcaaagtctc
    25441 ggtgaacgat aaagcttaat gcatcgactt tttctccatt cagcaagatg tccatcttca
    25501 ctaacttgct tttttgataa ccagacattt cataatccaa tgaggcatag cctttcgtgc
    25561 ttgatttcaa tttgtcgaaa aagtcaaaga caatttcaga aagcggaata ttatagacga
    25621 catttactcg gtaatcatct aaataatcca tcgtaatgaa ttctccccgt tttcgttgag
    25681 aaagttccat tactgcaccg acaaaatcat ttgggaccat gatctgtgct ttgacgaatg
    25741 gttcttctac atcctgaatc gttacaggtt caggaaaatc tgccgggtta tctactgtag
    25801 cagtcgtacc atccgtttta ttaacatggt aaataacgga tggtgcagtc gtgatcaatt
    25861 ctagattgaa ttcccgttcc aagcgttctt ggacgacatc catatgaaga agtccgagga
    25921 atccacagcg gaacccaaaa cctaatgctt gagaggtttc tggttcgaat tgtaaagcag
    25981 catcattcaa ttgcagtttt tctaaagctt cccgcaaatc gttgtaacga gaagtatcaa
    26041 ttggataaag accacaataa accatcggat tcatttttcg gtaacctggt aaagcttcag
    26101 cggcaggatt gtctgctaac gttactgtat cccctactcg tgtatcttgg acagttttga
    26161 tacttgctgt gatataaccg acatcaccga ccatcaaaaa gtctctagca actgcttttg
    26221 gcgagaagac cccgacctct gtaacatcaa atgttttgcc attactcatc agtttgattt
    26281 tgtctccggg tttgaccata ccgtccgtga tccggacatt tagaacaaca ccgcggtaac
    26341 tatcataaat agagtcaaag atcaaagctt ttagcggtgc atctaagtct ccacttggtg
    26401 caggaacata ttccacgatc tgctcgagaa tatcttcaat cccaatccct gatttggcac
    26461 tggctaatac agcttcacta gcatcgattc cgattacgtc ttcgatttct tgacggacac
    26521 gttcgggatc tgctgcgggt aggtcgattt tattgatgac tggcaggatc tccaaatcat
    26581 tgtctaacgc taaataaaca ttggccaatg tttgtgcttc gattccttgt gctgcatcga
    26641 ccactaaaac ggcaccctca caggcagcaa ggcttcgaga gacttcatag gtgaagtcga
    26701 cgtgccctgg ggtgtcaatc aagtgaaaga tatatgtttc tccatttttc gctgtgtaat
    26761 tgagttctac agcatttaat ttaatcgtga ttccacgttc acgttccaaa tccatcgagt
    26821 ctaacagctg atcttgcatt tcgcgagacg tcacagtgtg cgtcatttct aaaatacgat
    26881 cggccaatgt agatttccca tggtcgatat gagcgatgat cgaaaaatta cgaatctgct
    26941 cttggcgttc tttcattttc tttatatcca tagcgtctcc tcttttcata taatcagcac
    27001 tttccattat accaacgaaa agcagtaatt taaagagttt ttaattagtc agctatagaa
    27061 gttttttttg tagaattttg ctatactgtt atcaagctaa taaatggaga tgaaaaaatg
    27121 gatcaaaaag attttagaga aaatcttgaa ttaaaaccgg tcactcaaga acatatcgat
    27181 caattcaatg aattgctttc ctatgtgttc caagtgactg aagcagacat cgaggaaagc
    27241 gggtttgaga ataaacgagc ttttatccgt tccaaaaaac cgattttgga agtatcgaaa
    27301 gtgttcggat ggttccacga aaataaactg atctctcaaa tcgctatcta tccttgtgaa
    27361 gtcaatattc acggtgcacg ttataaaatg ggtggtgtga ctggagttgg aacttatcca
    27421 gaatatgcta accatggatt gatgcaagat ctaattatcg ttgcattgga taatatgcgc
    27481 aagaataagc aatggatctc ttatttgtat ccttacagta tcccctacta tcggcgtaaa
    27541 ggatgggaaa tcatgtctga taaactttcc tttaaaatca gagatacaca gctacctaaa
    27601 caaatcccag taagtggtat ggttgagcga ttaccagttg atcatccaga cgtattttcc
    27661 gtttatgaca aatttgccca acaaaaccat ggtgctttat tccgcagtga ttttcattgg
    27721 gaagagtatt ggcgtttcga aaatgaagat gagcgcacag ccgctgtcta ctacgatagc
    27781 aaccatgaac caagaggtgt tctgttctac tgggtagctg aagaaatatt tcacgtaaaa
    27841 gaaatgttct atcttgatca agaagctcga aatggcttat ggaactttat ttccgcacat
    27901 ttttccatga tctactgggt acatggagat atttataaaa atgagccttt agcatttttg
    27961 attgaagata gtcagatcaa agagcagatc gaaccatatt ttatggctcg gatcgttgat
    28021 gtaaaagaat tccttcaacg gtttccgttt gttggaacag ctgatgcttt tcatttcatc
    28081 attgaagatc ctgtcgctcc ttggaataat ggtgtcttcg ctttaacatg ggatgaacag
    28141 ggacaagtcc gtgtcttaaa tgaacctata ggaaaatccg ttcgactgga tatccaaaca
    28201 ttgacttgct tgatgatgaa ttatcgccgt gcttcttatt tggcaagaat cgaacggtta
    28261 gaaacagatg aagaaacact gaagtcacta gagcgtatca ttccaaacat ggaagcttac
    28321 tttagcgatt atttttgata aaaaggatga tgtttagatg aaaatttatt ttgccgcacc
    28381 aatgtttgct aaaagtgatt tgttatataa ccaacagtta gttaaagaaa tcagagagct
    28441 ctcttctgag ctttcgatct atcttcctca agaaaatgaa gcaatcaatg acaaaactgc
    28501 ttatgcagac agtcgaatga ttgctcttgc tgacacagaa aaagtgttgg ctagtgattt
    28561 aatgattgct ttactagatg ggctgacgat cgatgccggg gtagcatcgg aaatcggtgt
    28621 ggcttatgcg aaaggcatcc cagtgatcgg cttatatact gatactcgac agcaaggcgg
    28681 aacccacccg aaaaaaatcg ctgcactaca agaaacagct gaaaatcaat ttcactacct
    28741 gaacttatat acaataggtt tgatcaaatt gaacggaaaa gtggtttctt cagaaattga
    28801 gttgctttca gaagtaaaaa gatttttaga tggagggact ttcagtgatt aaagaaatca
    28861 aaaaggtgca aatagcttta cttgcttttg gtgtattcgt tgtctgctat aatttttatg
    28921 aatttatcac acaaaaatat tcgacttctc aaggaatcac atttatcgtg gaatctttat
    28981 tagggattgc attgattttc atgccacaag tcattttgaa agttttcaaa cttaaaatac
    29041 cagcagcaat cgttttgttt tactggtttt tcttgttcat ctctgtcttt ttaggcacag
    29101 ggatgcattt gatcagtatc atctcttttt gggacaaaat cttacatgcg gtgagtccaa
    29161 tggtcctgac agctcttggc tatggattga ttggttactt gatgaaagat gctgagatat
    29221 caaaaaccag tccttggttg ttcctcttat tcggctttgc gtttgctgga ctttgcggcg
    29281 tgttttggga gttctgggaa ttcttatgcg accagttctt aggcatgaat cttcaacgtt
    29341 ttgctgcttc tgatggcaca ctgtttgtcg gacgagcagc attgatggat acgatgggcg
    29401 acttgctgac aaatacgata ggtgctgcct taatgggatt gtttgcatgg tctcaaagta
    29461 aaaaagatga gcgttacttt gaaagttata aattagaaaa agtgaaaaat acctaataaa
    29521 agaggttgtg acagaagcgg gcagcttcaa gaaatttcgg cttatttttc gaagaaacta
    29581 cttctggaac actgtttatt tgaaaaaagg aggtctgaca ctagttgtgt cagacccctt
    29641 ttttctttta tagtctcaat ggaagattag acgatttctt ctagctcttc tgttttgaaa
    29701 tgaagttgtc catcaagtaa agtaatcgtt acttttgatt taggcataac atgtcctgca
    29761 acgatttctt tagctagtgg tgtttctact tccttagtga taaatcgttt caatggtctt
    29821 gcaccatatg ctggttcata agcattttca gcaatccacg ttttggcttc atcactgatt
    29881 gtcagcagga tttcttgatg ttccagacgt tgtgccaatt gcgccaccat tttgtcaacg
    29941 attcctttca cgttgtctag gctcaatggt gtaaataaaa tcgtgtcgtc gattcggttc
    30001 aagaattctg gtttgaagtt tccacgtaat agcgtgttga cttgttcagc cactgcttct
    30061 ggaatcgttc catcagcagt cacgccttcc agcagtaact gtgaaccgat attgctggtc
    30121 atgatcagaa ccgtattttt gaaatctact actcgccctt ttgaatcagt caagcgtccg
    30181 tcatctagga cttgtaacaa gatattgaag acatcgggat gcgctttttc gatttcatcc
    30241 aacaagacga ttgtataagg atttcgtctg acagcttccg ttaattgacc accttcttcg
    30301 taacctacat agcctggagg agctccgact agacgagaca cagcatgttt ttccatatat
    30361 tcactcatat cgatccgtac catatgatct tctgagtcaa acaagttctc agctaacgct
    30421 ttcgctaatt cggtttttcc gacaccagtt ggtcctagga aaaggaaaga acctagagga
    30481 cgatttgggt cttgcaatcc agcccgtgaa cgaattaccg cgtcactgac cgcatcaact
    30541 gcttcatctt gaccaatcac tcgtttatga agtgtttcat tcaatttaat tagtttttct
    30601 cgttcacctt ctacaagttt cgttacgggg atacccgtta atctgccaac gacttgagca
    30661 atctcatttt cggtaacgga ctcttgaacc atcttaatct cgctgtcttt tgctttagct
    30721 tctaattcct tcaattcttt ttctaattga ggtatcgtac catgacgcaa tacagcagcg
    30781 cgttctaaat catagttatt ttctgcatct tctaattcgt gttttgcttt atcgatttca
    30841 gcacgttttg ctgaaacaga attgacttct tctttttctg tttcccactg catcttcatc
    30901 gcatttgctt cttcacgaag ttcagctaat tcttcttgca aatttttcaa gcgtttttta
    30961 cttgcatcat ctgattcttt tttcagtgca gcttcttcga tttccaactg catcaaacgt
    31021 ctagtaactt gatctaattc cgttggcata gagttcattt ctacccgaat cgttgcactg
    31081 gcttcatcga tcaaatcgat tgctttgtcc ggtaagaacc gatcagtaat ataacggtca
    31141 gataaagtag ctgctgctac taatgcgttg tcatggatgt taacaccatg gtggatctca
    31201 aaacgttctt ttaatccacg cagaatactg atcgtatctt ccactgttgg ttctttgact
    31261 aatacctttt gaaagcgacg ttccaacgct ttgtcttttt ccatatattg acggtactca
    31321 tctaaagttg tggcaccgat caaatgtaat tcaccgcgag caagcattgg tttcaacaga
    31381 tttccggcat ccatacttcc ttctgttttt cctgccccta cgatattgtg gatttcatcg
    31441 atgaacaaga ggatacgtcc atcacttttt ttgacttctt tcaagactgc ttttagtctt
    31501 tcttcaaatt caccacggaa ttttgctcca gcgatcaatg cacccatatc taatgaaaaa
    31561 atcgttttat cttttaaatt ttcaggcaca tctttgcgta cgatacgttg agcaagccct
    31621 tcaacgattg ccgttttccc tacaccgggt tctcctatca gaacaggatt attttttgtt
    31681 tttcgtgaaa gaatacggat gacatcacgg atttcttcat cacgaccgat gattggatcc
    31741 atttttccgc ttttgacttg ctgtactaaa tcaacgccgt atttttctaa tgctttgtat
    31801 tgttcttctt ggttttgtga ggtcactctt tctcctcctc tcatctcttc gatgttttta
    31861 cgcaattctt tttcagataa tccgttttta ttcaaataaa cagttaacgg ataatttttt
    31921 aatttcatca acgctaaaat aacgacttcc gttgaaagaa actcgtcgcc gaagctttca
    31981 cgtatttggt ctgcttcatt caataatcga aagaaattct ggctgaggtt ttgtccatat
    32041 tggacgtttc caccagaaac aactggatac tcatccagta gacgatcaat ttcatgttca
    32101 aaagattcga catccaatcc ggcatccgta tagaaatttc gaccaaaatg attagattgt
    32161 aaaaagattt tccatacatg ggcaatgtcg atatcttgat gttttcgagt gaccgcaatc
    32221 tgttgagctt cagcaatcgc ttcttgaagc gtcgtagtca ttttctcaat attcatacag
    32281 gtataccctc ctaattaaag gtaagatcac ttatttcttt gatctttaca aaaactatta
    32341 tatcgctttg gtcaattttg gtcaaaatat acacatagtt ttttgacttt ttttctcgac
    32401 ttatataaat
//
LOCUS       DAZUMT010000020        32258 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000020 DAZUMT010000000
VERSION     DAZUMT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.020"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8FM_000857"
     CDS             complement(99..1229)
                     /locus_tag="ACG8FM_000857"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS5093539.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8FM_000858"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8FM_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5093540.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8FM_000859"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8FM_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5093541.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACG8FM_000860"
     CDS             complement(3485..4549)
                     /locus_tag="ACG8FM_000860"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5093542.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8FM_000861"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8FM_000861"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093543.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8FM_000862"
     CDS             4880..5692
                     /locus_tag="ACG8FM_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093544.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8FM_000863"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8FM_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093545.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACG8FM_000864"
     CDS             complement(6502..6732)
                     /locus_tag="ACG8FM_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093546.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACG8FM_000865"
     CDS             complement(6716..6913)
                     /locus_tag="ACG8FM_000865"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5093547.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="ACG8FM_000866"
     CDS             complement(7059..7937)
                     /locus_tag="ACG8FM_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5093548.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="ACG8FM_000867"
     CDS             complement(7976..9094)
                     /locus_tag="ACG8FM_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5093549.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="ACG8FM_000868"
     CDS             complement(9091..11190)
                     /locus_tag="ACG8FM_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS5093550.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="ACG8FM_000869"
     CDS             complement(11241..12062)
                     /locus_tag="ACG8FM_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5093551.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="ACG8FM_000870"
     CDS             complement(12059..12868)
                     /locus_tag="ACG8FM_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5093552.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="ACG8FM_000871"
     CDS             complement(12889..13383)
                     /locus_tag="ACG8FM_000871"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5093553.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13895)
                     /locus_tag="ACG8FM_000872"
     CDS             complement(13395..13895)
                     /locus_tag="ACG8FM_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5093554.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13960..16656)
                     /locus_tag="ACG8FM_000873"
     CDS             complement(13960..16656)
                     /locus_tag="ACG8FM_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5093555.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="ACG8FM_000874"
     CDS             complement(17084..18157)
                     /locus_tag="ACG8FM_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5093556.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="ACG8FM_000875"
     CDS             complement(18154..18981)
                     /locus_tag="ACG8FM_000875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093557.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="ACG8FM_000876"
     CDS             complement(18982..19788)
                     /locus_tag="ACG8FM_000876"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093558.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="ACG8FM_000877"
     CDS             complement(19800..20885)
                     /locus_tag="ACG8FM_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093559.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="ACG8FM_000878"
     CDS             complement(20902..21444)
                     /locus_tag="ACG8FM_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS5093560.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="ACG8FM_000879"
     CDS             21702..22691
                     /locus_tag="ACG8FM_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5093561.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="ACG8FM_000880"
     CDS             22688..23425
                     /locus_tag="ACG8FM_000880"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS5093562.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="ACG8FM_000881"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="ACG8FM_000881"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS5093563.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="ACG8FM_000882"
     CDS             23989..24621
                     /locus_tag="ACG8FM_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5093564.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="ACG8FM_000883"
     CDS             24706..25353
                     /locus_tag="ACG8FM_000883"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5093565.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="ACG8FM_000884"
     CDS             25358..26341
                     /locus_tag="ACG8FM_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS5093566.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="ACG8FM_000885"
     CDS             complement(26744..28258)
                     /locus_tag="ACG8FM_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS5093567.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="ACG8FM_000886"
     CDS             28396..29202
                     /locus_tag="ACG8FM_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093568.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="ACG8FM_000887"
     CDS             complement(29377..29946)
                     /locus_tag="ACG8FM_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5093569.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32088)
                     /locus_tag="ACG8FM_000888"
                     /pseudo
     CDS             complement(30029..32088)
                     /locus_tag="ACG8FM_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
BASE COUNT         9661 a         6754 c         5490 g        10353 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttaccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt tttctgacaa
    30481 gctttcttct tgtctttttt gttctttttg atctatctgt ttgtttaatg gtaaaacatg
    30541 taatagtgac tgagcatgga gttcttggag tgaatacggg tcatgataac tatacataat
    30601 cgtaaaatca cctgtttgtt tggtcaaata accatcctcc aatggtactt cattgattgt
    30661 aaccgataca tcctttaacg gtacgggttt acctaaggaa tttgttgctg attggatcaa
    30721 ctcttctttg tttatttttt cgtttggatg aacaataaga tcatgacctt ctaatgaacg
    30781 attgtctggt atataaacat aagtaaaggt aatttcccct cctgattgct tcctattatt
    30841 catcagttct gagtgaagta cttcaaggat ctccgaccag tgactttttc tgtctaatcc
    30901 gtttgcttgc atccaatcag aaatagaagt gactttccca ttttgttcat aagtcgataa
    30961 atctatattt ggcactcgat cagaaaattc agcaggtagg cgcaaatcag gaatacgatt
    31021 tacactactc atatgtgttt ctgcttgaag ttctttattt gtttgactat ctataaaatg
    31081 agtgatgaca gtcgatgaag cagcagatgc ttctacataa actacagctg ttgtttctgc
    31141 cacatttgat gttttcgagt ctatataagt taatacaacg ggatattctc ccggagttgt
    31201 agtatctact gtcccgttca ttttgatttc ttctttattt cctgaagttc catcgctttt
    31261 tgttacatat tcaacaagat ctttaggggc gtaactctct cctgttctta agtgaagatc
    31321 ttttcctttg atagctgttt gatcagcacg aacatggatc acagcagatg cagtcattgt
    31381 ttttaaagca attggatcaa aataggacaa ggaaatagta gatgttcctg tattagtcgt
    31441 atccacgata ttcccattgt ttgaaattac tttggacata tctccttgtg ttccatctgc
    31501 gttcttcagc ctttctacca gatcagatat cgaaactttt gtccctgcca gaacatcgat
    31561 tttctttctt tcaaactgtg aataatcttt ttcatataca taagaaactt gtgcgcttcc
    31621 accgtccttt atatctgtca aagcatgatt caaaatatca gtgatctgtt ttaactgatc
    31681 accttttgta ggatcagtca tatcggaaaa gatagaaagc ggcacactgc ttccggtttc
    31741 cgatgtccat aatgttttgt taggaattaa ccagtagtgg tttatcgttg gaggataact
    31801 gactttatta taggaagtca atgcaggaag tagctgtttt ccatttgcat cagtgaaagt
    31861 atgagtaata gtgatagggg aatcagtggt ctcagaaaca gaggtacgta cgaccttgat
    31921 ggtttgtgtc gttgcctgaa cgggtacatg catatagaca cctacagata aactgatcag
    31981 catcgtgcgc atcataaact taacgtagtg tttttcagaa cacgaccgtt ccttatctaa
    32041 acgacaagcg tgtttattta gttgttttga acgtcttttt gaacacattt attcttagtc
    32101 ctccctactc ttgtctaaac atgaagtagt aattttcata agatgatttc attatataaa
    32161 taaaatcaaa agaagatgga ctattaggag agtatttttt ttgatttttt attgtttcaa
    32221 gaaaatcgga ataataactt atgtactttt taattttg
//
LOCUS       DAZUMT010000021        30741 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000021 DAZUMT010000000
VERSION     DAZUMT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.021"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..251)
                     /locus_tag="ACG8FM_000889"
     CDS             complement(87..251)
                     /locus_tag="ACG8FM_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093570.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            546..1874
                     /locus_tag="ACG8FM_000890"
     CDS             546..1874
                     /locus_tag="ACG8FM_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS5093571.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2076..2909
                     /locus_tag="ACG8FM_000891"
     CDS             2076..2909
                     /locus_tag="ACG8FM_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS5093572.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2925..3662
                     /locus_tag="ACG8FM_000892"
     CDS             2925..3662
                     /locus_tag="ACG8FM_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS5093573.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3817..4785)
                     /locus_tag="ACG8FM_000893"
     CDS             complement(3817..4785)
                     /locus_tag="ACG8FM_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS5093574.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5017..5850
                     /locus_tag="ACG8FM_000894"
     CDS             5017..5850
                     /locus_tag="ACG8FM_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS5093575.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5884..6723
                     /locus_tag="ACG8FM_000895"
     CDS             5884..6723
                     /locus_tag="ACG8FM_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5093576.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6749..7309
                     /locus_tag="ACG8FM_000896"
     CDS             6749..7309
                     /locus_tag="ACG8FM_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5093577.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7508..9229
                     /locus_tag="ACG8FM_000897"
     CDS             7508..9229
                     /locus_tag="ACG8FM_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS5093578.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9394..10536
                     /locus_tag="ACG8FM_000898"
     CDS             9394..10536
                     /locus_tag="ACG8FM_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5093579.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10552..11763
                     /gene="thiI"
                     /locus_tag="ACG8FM_000899"
     CDS             10552..11763
                     /gene="thiI"
                     /locus_tag="ACG8FM_000899"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS5093580.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12336..12983
                     /locus_tag="ACG8FM_000900"
     CDS             12336..12983
                     /locus_tag="ACG8FM_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5093581.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13112..13328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13376..16021
                     /locus_tag="ACG8FM_000901"
     CDS             13376..16021
                     /locus_tag="ACG8FM_000901"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS5093582.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16331..17644
                     /locus_tag="ACG8FM_000902"
     CDS             16331..17644
                     /locus_tag="ACG8FM_000902"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS5093583.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17634..18287
                     /locus_tag="ACG8FM_000903"
     CDS             17634..18287
                     /locus_tag="ACG8FM_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5093584.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18342..19034
                     /gene="radC"
                     /locus_tag="ACG8FM_000904"
     CDS             18342..19034
                     /gene="radC"
                     /locus_tag="ACG8FM_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS5093585.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19184..19384
                     /locus_tag="ACG8FM_000905"
     CDS             19184..19384
                     /locus_tag="ACG8FM_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5093586.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19638..21317)
                     /locus_tag="ACG8FM_000906"
     CDS             complement(19638..21317)
                     /locus_tag="ACG8FM_000906"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5093587.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21319..21531)
                     /locus_tag="ACG8FM_000907"
     CDS             complement(21319..21531)
                     /locus_tag="ACG8FM_000907"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS5093588.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21924..23654
                     /gene="efrA"
                     /locus_tag="ACG8FM_000908"
     CDS             21924..23654
                     /gene="efrA"
                     /locus_tag="ACG8FM_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS5093589.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23651..25411
                     /gene="efrB"
                     /locus_tag="ACG8FM_000909"
     CDS             23651..25411
                     /gene="efrB"
                     /locus_tag="ACG8FM_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS5093590.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25506..25703
                     /locus_tag="ACG8FM_000910"
     CDS             25506..25703
                     /locus_tag="ACG8FM_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093591.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25856..26341
                     /locus_tag="ACG8FM_000911"
     CDS             25856..26341
                     /locus_tag="ACG8FM_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS5093592.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26445..27038)
                     /gene="clpP"
                     /locus_tag="ACG8FM_000912"
     CDS             complement(26445..27038)
                     /gene="clpP"
                     /locus_tag="ACG8FM_000912"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS5093593.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27218..27745
                     /locus_tag="ACG8FM_000913"
     CDS             27218..27745
                     /locus_tag="ACG8FM_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093594.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27836..28573
                     /locus_tag="ACG8FM_000914"
     CDS             27836..28573
                     /locus_tag="ACG8FM_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5093595.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28577..29494
                     /gene="miaA"
                     /locus_tag="ACG8FM_000915"
     CDS             28577..29494
                     /gene="miaA"
                     /locus_tag="ACG8FM_000915"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS5093596.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29496..30725
                     /gene="hflX"
                     /locus_tag="ACG8FM_000916"
     CDS             29496..30725
                     /gene="hflX"
                     /locus_tag="ACG8FM_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS5093597.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10151 a         5259 c         6561 g         8770 t
ORIGIN      
        1 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
       61 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      121 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      181 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      241 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      301 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      361 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      421 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      481 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
      541 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
      601 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
      661 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
      721 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
      781 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
      841 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
      901 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
      961 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1021 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1081 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1141 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1201 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1261 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1321 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1381 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1441 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1501 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     1561 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     1621 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     1681 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     1741 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     1801 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     1861 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     1921 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     1981 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2041 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2101 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2161 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2221 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2281 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2341 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2401 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2461 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     2521 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     2581 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     2641 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     2701 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     2761 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     2821 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     2881 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     2941 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3001 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3061 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3121 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3181 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3241 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3301 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3361 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3421 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3481 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     3541 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     3601 atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat
     3661 aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat
     3721 tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag
     3781 cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa
     3841 ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact
     3901 attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata
     3961 atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt
     4021 ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa
     4081 agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg
     4141 catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg
     4201 ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg
     4261 acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg
     4321 tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca
     4381 acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact
     4441 tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa
     4501 aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga
     4561 tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg
     4621 tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc
     4681 tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt
     4741 catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt
     4801 acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat
     4861 cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa
     4921 aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa
     4981 agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg
     5041 tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg
     5101 tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt
     5161 gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga
     5221 atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat
     5281 tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa
     5341 ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag
     5401 tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa
     5461 tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat
     5521 gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt
     5581 gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg
     5641 cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa
     5701 gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt
     5761 ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga
     5821 ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag
     5881 tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa
     5941 gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt
     6001 gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat
     6061 atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac
     6121 ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa
     6181 atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg
     6241 atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa
     6301 aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata
     6361 ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta
     6421 gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc
     6481 ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa
     6541 atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta
     6601 ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg
     6661 actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat
     6721 tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga
     6781 aacattccac aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa
     6841 tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac
     6901 ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa
     6961 agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt
     7021 agatgcactg acagatatcc tttactttac ttatggttct ttttcattac taggaatagt
     7081 gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg
     7141 aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga
     7201 atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc
     7261 taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca
     7321 ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg
     7381 catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt
     7441 ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga
     7501 accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat
     7561 tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt
     7621 agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa
     7681 aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa
     7741 tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct
     7801 caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat
     7861 ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac
     7921 ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga
     7981 aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat
     8041 caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt
     8101 tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat
     8161 gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga
     8221 aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct
     8281 tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa
     8341 aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt
     8401 atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac
     8461 gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga
     8521 tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca
     8581 aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca
     8641 cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga
     8701 tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa
     8761 agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt
     8821 agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac
     8881 tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt
     8941 caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga
     9001 tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca
     9061 tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata
     9121 tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc
     9181 tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt
     9241 tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt
     9301 gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata
     9361 atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca
     9421 accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg
     9481 aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga
     9541 aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga
     9601 acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat
     9661 catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa
     9721 gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac
     9781 gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat
     9841 gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa
     9901 attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg
     9961 acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc acggggaact
    10021 ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa
    10081 gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct
    10141 ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta
    10201 gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac
    10261 tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat
    10321 gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat
    10381 atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt
    10441 cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc
    10501 aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac
    10561 agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt
    10621 atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc
    10681 catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt
    10741 ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa
    10801 gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa
    10861 gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa
    10921 ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg
    10981 cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat
    11041 gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg
    11101 ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa
    11161 attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca
    11221 aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca
    11281 tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt
    11341 actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc
    11401 attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct
    11461 atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa
    11521 atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat
    11581 tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat
    11641 caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag
    11701 atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta
    11761 taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag
    11821 ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta
    11881 tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag
    11941 agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt
    12001 tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa
    12061 tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga
    12121 ggttgcgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    12181 atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca ccgttttatt
    12241 tgtctcacac ccttttcctt cctacttgta accttgctcc cgtgttagaa acatgttaca
    12301 ctaaatatgt gaaattatta acaaggagcg tcgttatgcg agaaaaaaca gatacactgc
    12361 caaaagcaac tgcgaaaaga ttacctttat atttacgcta cttgaagatg ctggatgact
    12421 caggtataag tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt ccttctgcaa
    12481 cgatccgcag agacttctcg caattaggcg agctaggcag aagcggatat ggctatgatg
    12541 tcccttttct tatcgatgta ttcaataata tccttaatac taaagaagaa aaacgaatcg
    12601 cattagtagg atatggaaat cttggcaaag cattgaaaca caacaatttt agaagaaatg
    12661 aaaatctaaa tatcgtgtgc gtctttgata atgatccagc actgatcaat cgagtaattg
    12721 atggagaaat gatctatccg attgatcgtt tcgcggagat agccaaagca aaaaatgtga
    12781 cagtagcaat ctctacggta ccaagtaaat attcacaatc tgctattgat gaaattgtca
    12841 aaggaaatgt aaccgctata cttaattttg cgcctgacag agtaactgtc ccagcttatg
    12901 tcaatgttca atacattgat ttgacaaccg agttgcagac gcttatttac tttgatgaga
    12961 attactcaga agttttttct taggaaaatt tgagtaggct tattgatctt gcgagaactc
    13021 gtcgtaattc tttcaaaaat tttttagtac taaatgaagt ggttttttca cgaccacttg
    13081 ttgactaaaa aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga ggagtaactg
    13141 gcacattcat tcttagagag agaactatct gctgaaaggt tctcaatgac aaccagtgaa
    13201 ggtagcttgg gagtttctgt cttgaatgga gtagagatag acgagtcgtg atcgttaact
    13261 cacttgagag gcaataatga ttgattattg caatttaggt ggtaccgcgt aaatcatacg
    13321 tcctagaata cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa acagcatgac
    13381 ggaagaaaat aacctatcaa caaaatataa tccgcaagaa gttgaagctg gacgttatca
    13441 gcattggtta gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa aaccttattc
    13501 aatcgtaatc ccgcccccaa atgttactgg aaaacttcat ttagggcatg cttgggatac
    13561 gaccttgcaa gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata ctttatggct
    13621 gcctggtatg gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga agcttagaga
    13681 gcaagggatt tctcgttatg atctaggtcg tgaaaagttc atcgatcaag tgtgggaatg
    13741 gaaagaagag tatgcgagcc acatccgcga acagtgggct aaaatgggct tatctttaga
    13801 ttacagtagg gaacgtttta cattagatga tggattatct gaagcagtac gtaaagtttt
    13861 tgttacctta tacgaaaaag gtttgattta tcgtggtgaa tatatcatca actgggatcc
    13921 tcaagcacga acagctctat ctgatatcga agttattcat aaagatatcg aaggcgcctt
    13981 ttatcatatg agctatgaac tagcagatgg atcaggcgta gtagaaattg cgacaacccg
    14041 tccagaaacg atgttgggag atacggcgat tgctgttcat ccagaagacg agcgttatca
    14101 agcgctcatc ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc caatcattgc
    14161 agatgaatat gtagatatgg aattcgggac aggtgtagta aaaatcactc cagcacatga
    14221 tccaaacgac tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg ttatggatga
    14281 tgatggtacg atgaatgaac tagcaggaaa atatgctgga atggaacgtt ttgctgctcg
    14341 taaagcaatc gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg atacgatgat
    14401 ccacagtgtg ggtcattccg agcggacagg tgtcgttgtt gaacctcggc tgtctactca
    14461 atggtttgta aaaatggcgc cgttagcgaa aaaagccatt gataaccaag aaacagatca
    14521 agcagtggaa ttcttccctc caagattcaa tcaaacgttt ttacgttgga tggaaaatgt
    14581 ccatgattgg gtaatctcac gacaattatg gtggggacat caaattcctg catggtatca
    14641 caaagaaaca ggtgaaatgt atgtagggat ggaagcacca gcggatagtg aaaactgggt
    14701 acaagatcct gatgttttag acacttggtt tagttcagct ttgtggcctt tctcaacaat
    14761 gggctggcca gatacggaaa gcgaagatta taagcgatac ttccctacta gcactttagt
    14821 aaccggttat gacatcattt tcttctgggt aagcagaatg attttccaaa gcttggaatt
    14881 tactggcgaa cgtccatttg agaatgtctt gattcatgga ttgattcgtg acgaacaagg
    14941 gcggaagatg agtaaatcat taggtaatgg aatcgatccg atggaagtaa tcgaaaagta
    15001 tggagcagat gctctccgct ggttcttatc gaatggttct gcaccaggac aagatgtccg
    15061 cttcagctat gaaaaaatgg atgcttcttg gaactttatc aacaaaatct ggaatgcttc
    15121 acgtttcgtt atcatgaata tcgaaggaat gaccgtagaa gagatcgatt tatctggaga
    15181 aaaatcagtg gctgaccggt ggatccttac tcgtttgaat gaaacgatcg aacgagtaac
    15241 tgagctattt gatcgttttg aatttggtga agcaggtcga caactgtata actttatctg
    15301 ggatgatttt tgtgactggt acatcgaaat gagtaaagaa gtactttatg gagatgatgc
    15361 tgtttcaaaa caaaccaccc gtagcatctt agtccataca cttgatcaga tcctacgttt
    15421 attgcatcca atcatgccat tcgttacaga agaaatatgg gaacatattc ctcatcaggg
    15481 aaattctcta gtagtggcag aatatcctgt tgtacgtcca gagtttgatg atgaaacagc
    15541 ttctaaaggg atggaagtat tgaaagaatt gattcgagcc gttcgaaata ttcgttcaga
    15601 agtgaataca ccattgtcta aaccaattac gttgctaatt aaaacaaacg atgctaaaat
    15661 cgatcaattt ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc ccgaagagtt
    15721 gacgatatca agtgagatca ctgcgccaga tctagcgatg tcagctgttc tgacaggtgc
    15781 agaaatcttc ttgccgttag caggattgat caatattgaa gaagaaatca aacgattaga
    15841 aaaagaactg gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac tttctaatga
    15901 acgttttgtt tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa aagaaaaaga
    15961 ttatttagag aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta ctattcaata
    16021 agaatagaaa aagagagtgt gaaatcagct agctattact aaaagctagc tgattgaaca
    16081 tcgttacagg aaagagtttg agcctcactc ttagcttcga ttcataagga gatgtgacat
    16141 aaattttgtc gcatctcttt tttagtctac tcttattatg taggagaagg agggaggtgg
    16201 atcatgtatc aagagtcaat aaagtagttg ataaaatcgt tcctctctct gtttcttttc
    16261 tctttgtcat acttttattt ttcttatgtg ttctatttgt tataataaaa gagaaatggg
    16321 ggaattgttg atgacgattg aagaagcaat agaatggatc catagtcgat taccatttgg
    16381 ttcacgtcct ggccttgacc gagtcgaagc tttattagaa gtggtagggc atccagagag
    16441 aaaagtaaaa acgatccata tcgctggaac aaacggaaaa ggttctactg tagcttattt
    16501 gagaagccta ttagaagagg ctgggctgac agtaggtaca tttacttcac cttatatcga
    16561 aacgttcaat gaacggatcg caatcaatgg caaaagcatt ccagacaatg atttgatcca
    16621 gcttgtaaaa aaatatcagc cactgatcaa acaacttgat caatttgaag cagtagctgg
    16681 tattactgaa ttcgagacat taacagcaat ggcatttgat tattttttga tgaagaaagt
    16741 ggacgtagcg attatcgaag ttggcttagg cggcttattg gattctacca atgtagctgt
    16801 accaatgctg acgggaataa caacgattgg acttgatcat accgaaatac ttggagatac
    16861 aattgaagaa atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc cagtagtaac
    16921 aggaaatatt gccccagaag cttttactgt catccaacaa acagctaaag caaaaaatgc
    16981 accgctttat gtatacgggg aaaattataa aataaactat ctgcatcctg atgagatgtg
    17041 gggagaagta tttgaatttt ttaatgaaat aggaaagatc cctcaattaa ccactccgct
    17101 tttagggcaa catcaagctg aaaatgcagg agtagccatc gaattgtatt ttgtttactg
    17161 tcaattggaa aagctgcctt tccgagaaaa agatgttttg aagggattga agaaatctcg
    17221 atggccagga agaatggagc gtttgagtaa cgagccatta gttgttttag atggtgccca
    17281 taatacgcat gcaatgaacc ggcttgttga gaatttgaaa aaagaattca aaggatatca
    17341 aatcaacatt ttgttttccg ccttacgaac taaaaatgtt agagaaatgc tagaacgatt
    17401 gcagcaagta ccaaatgtcc atatttattt gacgaccttt gaacatccaa aagccatcga
    17461 attaagtgaa gtatcagatt tagcaagtga gagagtatca atcgtttcat tgtggcaatt
    17521 cggattagca gatatattgg aaaagatgac aaatgaagac gtattagttg tgaccggttc
    17581 actttatttc atttcagaag ttcgccattt attaatacaa ttaggaggag atcatgaagt
    17641 ataatgcagt gatttttgac atggatggtt tgctgttcga cacagagtta gtgtattacg
    17701 aagccacaca agtagtagca gacaagatgg gttttcctta tgataaagac ctgtatttaa
    17761 aattcttagg tgtttcagat gaagaagtat gggcgaatta tcatatgatt ttctcggaat
    17821 ttggcaaaac aagtgtacaa agattcatag atgattcata ccaggaaacg cttgaacgtt
    17881 tttcgcaagg agcggtacaa atgaagcccg gtgtgttgga actcctctct tttttagaag
    17941 aacatcagat tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt gaacttttat
    18001 tgaagaagaa caatctgttt gaacgatttg agacgattgt ttctgcagaa aatgttcaac
    18061 gagcaaaacc agacccagaa attttcttgt tagctaatca gaagctaggt acagcgaaga
    18121 aagaaacatt gattttagaa gattcacaaa atggtatttt agcagcttct gcggctgaga
    18181 tcccggtagt catggtacca gatctgcttc caccatccca agaattgatc aaaaaaacag
    18241 ctggggttgt ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa tccatctctt
    18301 ttcgtatctt attttagata agatagaaag gagatgtttt tttgggaaaa cgattgatca
    18361 aagaagtacc tactagctct ttaccaaggg aacgtatgga aatttatggc gcggaagctt
    18421 tatcggatca ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct tatagtgtga
    18481 tgtcgattgc tggaaatctg ttaaaaacgt ttggaggact ggcctcttta cgacaggcta
    18541 ctttgcatga gttagaagag attcgaggga taggacgcgt gaaagcaatc gaaatcaaag
    18601 ccttgatcga attaggtcga cgaattcaga cggatcatga aagagcggct ccaccggttc
    18661 gtaccagcta cgaattggcc cagcaattga ttctggaaat gaaagatcac aaacaagaac
    18721 atttggtttg tatttatttg gacacgaaga atcaagtgat cttaaaaaaa acagtgttca
    18781 tcggttcgct gaatcagtca gtcgctcatc ctagagaaat tttccattat gctgtacgtt
    18841 attgtgcagc aaggatcgtt ttagcgcata atcatccttc tggtaatgtt attccatcgc
    18901 agcaggatat gaattttacc aaaaggatac aaaagtgtgg agaaatgatg gggatcactg
    18961 tactggacca cttgatcatc ggacgcaaac gatattttag tttgagagaa gaaggaatga
    19021 tggaagagaa gtaagttgct ttcttgcaaa aaaaatgaag caagtgtaca attatattgt
    19081 aattttgttt gggaatagaa gttgaaacat ctgttgcacc tctatcgtac acgagcaatg
    19141 ttacaattta aattagtgct tttggcacga ggaggatatt attatggaaa acggaacagt
    19201 aaaatggttc aacgctgaaa aaggatttgg atttatctca cgtgaagatg gaagcgacgt
    19261 attcgttcac ttctcagcta tccaaggtga cggattcaaa acattagaag aaggtcaagc
    19321 agtgactttc gacgttgaag aatcagaccg cggcccacaa gctgcaaacg ttgtaaaagc
    19381 ttaattttat ctgtaaatca aattgatggt agacatcaat ttttacgaga gaacaaagcg
    19441 agtaacaaaa atacctgcca caagagacaa tttctcttgt ggcaggtatt tttttgtatg
    19501 aaaaagtaaa taaatttaag aggctatgaa agagccacct tgtatcaagg aataagaaca
    19561 aacaaaaatg gcttctcatg tttttcattt gttttctctt atattcagga caaggtgctg
    19621 atttcataac ctcttattta ttcttcatct ggtgtcatta ccattggcaa aatcattgga
    19681 tgtctttctg tacgttcgaa taagaaaggc tgaagcgcgc cagtcatcgc ttctttcaat
    19741 ttatattctg aacagtcagg tccattcaaa cattctctta aagcgtggaa taagattcgc
    19801 tgagcttcat tgatcatttc tcctgattct cgcatgtaga caaatccacg agagagaata
    19861 tctggaccag ccaagatttc ttttttcttg atatccactg tcgcgacagc taatacaaga
    19921 ccttcttctg aaagcaggtg acggtcacgt aaaacgacat taccaatatc accgatcccg
    19981 ctaccatcga tatacacatc tccagccaca aaatgcccag caggacgcgc agaatcgcta
    20041 gttaacgcta aaacatcgcc attttccatg atgaagcagt tttctgcagg aacacctaca
    20101 tcttgtgcta gagaagcatg tactttcaac atgcggaatt ctccgtggac aggaataaaa
    20161 tattttggct tcatcaaacg aagcattaat ttttgttctt cttgtccacc gtgaccggaa
    20221 gtatggatgt tattgatttt tccgtgaatc acttctgcac cagcttcaga cagcaaattg
    20281 atcaaatgat tgacacttgt tgtattacca ggaataggtg aacttgaaaa gatcactgta
    20341 tcgtctggct ggatttggat ttgacggtga gtaccattcg cgatgcggct caacgcggcc
    20401 atcgcttctc cttgagaacc tgtacatagg atcatcgttt cattggcagg caagcgattg
    20461 atttcgtgtg catctacaaa tgtgccgtct ggaactttga tatatcctaa acgttgaccg
    20521 ttgacgatgg cattttccat gcttcgtccg aaaacagcga ttttacgacc agttgccaca
    20581 gcggcatctg ctgcttgctg caaacggaag atgttagaag caaagctggc aaaaatgata
    20641 cgtccttcga tttttgaaaa gatcttcaaa atagaatgcc caatcgtttt ttctgattta
    20701 gtaaatgttg gaatctcagc atttgtacta tcagataaaa ggcacaagac tccttcttcc
    20761 ccgatacgag ccattttatg caagtttgct ggttcgccaa caggagtgaa atcgaatttg
    20821 aagtctccag tcgctacgat atttccagaa ggtgttttca ccacgatccc taatgcatct
    20881 gggatactat gggtcgttct gaaaaagctg acagaagtct ttctaaaacg gatgatcgta
    20941 tcttcattga tctcatggag ctctgcatct cgaagcaagc catgctcatc taatttgttt
    21001 gtgatcaacg ctaatgcgag aggaccagca tagataggaa tatttgcttg tttcagcaag
    21061 tagggtattc cgccgatgtg gtcttcgtgc ccgtgagtaa tcaccaaggc ttttactttg
    21121 tgtaagtttt gtatgatgta gctgtagtcg ggaatgacat agtcaattcc aagtaaatcg
    21181 tcttccggga atttgatccc ggcatcaatc aaaatgattt catcttggaa ttgtacccca
    21241 tacgtatttt tcccgatttc acctaatcca ccaatagcaa agacgcctgt ctcgttattt
    21301 ttgatggaaa gtttcatatt aaaactccac taatgagaaa tcagcatttt ctttttcgta
    21361 ctctagatga cttccttcta gaagttgaac gaattcaata ttgtatgggg tattttcttc
    21421 cacacgttgg cgagcgtcta catcgctttt tgcctcaatg taaagagatt ttgtttcttc
    21481 ccgtttaggg ttgcgtactt ttgtttcttg ataataaact ttatagatca ttgttttgtc
    21541 tcctttatct gcgtaaagca gtgatttagc tagttgattt ttttagacaa cacaaaacga
    21601 ggggacaagc cacctggttt ttcatatgct ttgactagaa tactggattc gagtcaaaga
    21661 gacaagcctc gtttttgctt atctgttcgg atattttttc aaccgtacct tctgtataaa
    21721 aaatatacaa caattaattg ttattctagc ataaaacagc aaataagtat agaaatcaac
    21781 tagattgaaa gaaaaaatca atgtttatca tcccgctata atcataatga cttttttatg
    21841 agagaagtgt tctattaata ggctcgttgt gctatcattt ttagtaatga aaatgtaaca
    21901 aaagattaaa aggagatgag aaagtgaaac taatgtggca ttataccatg cgttataaga
    21961 aattcttgtt tttcaatttc atatgcgtat ttggttttat tttgattgaa ttaggacttc
    22021 ctacgatttt agcacgtatg atcgatgtag gaatccgcaa tgatgatttt gattatgtga
    22081 agcaacaagg gatatttatg atcgttatta ctgtaatcgg tattattttg aatatcttgt
    22141 taggttattt tggttcgagg attacgacta atatcgtagc agatattcga gatgatttgt
    22201 tcgaaaagat ccaaagttat tcgcatcagg aatacgaaaa tctaggcgta tcttcattga
    22261 tcactcgtac aacaaacgat gcttatcaga tcatgttgtt cctgcaaaat attttacgta
    22321 tcggttttat ggcaccaatg atgttcattg tcagtttgta catggtcatg cgtacaagtg
    22381 tgacactcag tttgtatgta gtaggtgcat tacctttact attattggca gtcgtagcga
    22441 ttgctaaggt gtctgaaccg ttatctaaaa agcaacaaaa aaacttagat aaaatcaaca
    22501 gtatcttgcg ggaaaatctt tcaggattgc gtgtgattcg tgcattcgtc aatgaaaagt
    22561 ttgaagaaaa acgttttgct gtagtcaatg agaattatgc ttctagttca aaaagcttat
    22621 tccgtttgat ggctgttgca caaccgggtt tttatttctt atttaatatc gtaatggtat
    22681 tgatcatctg gagcggggca gtgcaaatcg atgcaggcga gcttcgtgta ggagatttga
    22741 ttgcgtttat cgagtatata ttccatgcct tgttctcatt catgttattt gctagtgtgt
    22801 tcatgatgta tccaagagca gctgtttcag ctggacgtat ccaagaagcc ctagatgcca
    22861 aaccttcgat tgtagaagat ccaaatggta tatctgatac aaaaactaaa gggtatatcg
    22921 aatttcaaaa tgtaacattt gcttatccag gccattcgca agagccagtg atacgaaatg
    22981 tcagttttac agcctctcca ggagagaccg ttgcttttat tggaagtacc ggtagcggaa
    23041 aatctacgtt gatccaattg atccctcgct tttatgatgt gagtgaagga aagatcttga
    23101 tcgatggagt agacgtgaga gattatcggc taagcaagtt gcgagataag attgggttta
    23161 ttccgcaaaa agcgctgcta tttactggaa cgattgcaga taatttgcga tacgggaaag
    23221 aagatgctac gcaagaagaa atggaacgtg cagctgagat tgctcaagct tcagaattca
    23281 tttctcgaaa accagatgga tttaatgaac atttatcaga aggtggttcc aatttttcag
    23341 gtggacaaaa acaacgattg gcaatagcca gagccatcat acgccgtccg gaaatctata
    23401 tttttgatga cagcttttct gcattagatt atcaaacgga tgcgaagtta cgtgctcgtc
    23461 tgaaaaaaga aacgacagaa tctactgtat tgatcgtagc acaacgtgtg ggaacgatca
    23521 tgcatgcaga taagatcatc gtattaaatg aaggagaagt cgtcggtatg gggacgcatc
    23581 gggagttgtt ggaaaaatgc ccgatttatt atgatattgc cgcttctcaa ttatcaaagg
    23641 aggaattagc atgaaaaaaa ccttttcttc cttaaaacgt ttagctcgat atatcagacc
    23701 gtatcgtgag acttttcttt tagtgatcct atttacagtt ctgacagtgg ctttcaacgt
    23761 ggctttgcca tatgttacag gacttcctac tacagaaatc agccgtaata ttgccaatgg
    23821 cgaatcaatc aatttttctt atatattcaa atgtctcatt tggatcacag ttgttggaat
    23881 agggtattgt atttcgcaat tactttctgg tgttttgatg acaaatgtcg ttcaaagtgc
    23941 catgcatgat ttgagaagag acatagaaga aaaaatcaat cgtcttccgg tttcatattt
    24001 cgataaaaac cagcaaggaa atattttgtc tcgtgtgacg aatgatgtag atgcagtcag
    24061 cggtgcttta caacaagctt ttattggtat cgtaaatgcg atattgggaa ttacgttggc
    24121 tgcagcaatg atgttttata tccagccgat gatggcgctg atctctatga tcatgattcc
    24181 aacatcgatc tggatctcaa aacgagtgat caatgcttcg cagaaatatt ttcaaagcat
    24241 gcagaattct cttggtgagc taaacggcta cgtccaagaa aatatgacag gttttagtgt
    24301 attgaaagta tatggacgcg aaaaagaaac attggaagga ttcaaaaaag ttaatcacag
    24361 cttgaaatac tttgggtttc gtgcagcatt catttctggc ttgatgatgc ctctagttca
    24421 attaactgcc tatgctactt atattggtat ggctgttcta ggaagctttt atgttattac
    24481 tggggcgatc gttgtcggtc aactacaagc attcatccaa tatatttggc aaatcagtca
    24541 gccaatgggg aacgtgacac aattgtcttc agttttgcaa agtgcatcag cggcaacgaa
    24601 acgtgtattc gagattttag atgagccaga agaaaaagaa aacaaagtag atgcttcact
    24661 gccagaaacg attttaggag atgtgacatt cgatcatgtc gattttgcat atgatccgaa
    24721 gaaaccatta atccaagatt tgaattttga agtgaaagct ggtcaaaccg tagctgtggt
    24781 cggtccgacc ggtgccggca agacgacatt gatcaatcta ttgatgcgtt tttatgacgt
    24841 agacaaagga gcaataagga ttgatggaat cgacaccaaa tcaatgagtc gcagtgatgt
    24901 gcgttcgcta tttggtatgg tcttgcaaga tgcatggctt tatcaaggaa cgattgcaga
    24961 taatatccgc ttcggaaagc ttgatgcgac agattatgaa gtagttgatg ctgcgaaaac
    25021 agcaaacgta gatcatttta tccgaacgat gccagatgga tatgatatgg ccatcaattc
    25081 tgagggagac aatgtgtctt taggacaaaa acagttgctg acgattgccc gagcagtggt
    25141 ctccgatcct aaaatcttga ttttagatga agcaacaagt tcagtagaca cccgtttaga
    25201 agcgttgatt caaaaagcaa tggatcgcgt gatggaggga cggacaagtt tcgtcatcgc
    25261 tcaccgttta tcgacaatcc gtgatgcaga tctgattcta gtgatggatc aaggtcagat
    25321 cattgaaaaa ggaacgcatg agagtttatt ggcacaaggt ggattttatg aaaaactgta
    25381 taacagccaa tttgctgagg aagcagaata agtcatataa gaataaaaca gtttaaaact
    25441 tattttttgt tctctattct tgataatggt aaagtaaagt tatcaagaaa agtgaggaca
    25501 cggagatgga acagttttta aatgaaaaaa ttactgaagc caaaagagaa gttgatcgtt
    25561 taacgcacgt ctgtcagacg aagccttttg attcagctga tttttttgaa aatgagtttc
    25621 aaatcgaacg cttggcagca caaatagaag tgtacgaatt aattttaaaa aaagtaatag
    25681 aagaaaatat agatgtacaa taaaaacagg taaaacgtca gttaccagat actgtaaata
    25741 gacatctggc ctgagcaggt tttacctgtt ttttttctta tgaaataaat atatgctatg
    25801 atacctttat tcaataggat tagtttctac aaaaaatgaa aggagaaaaa taaatatgga
    25861 acggtttata gcatcaaatg ccacagtgat tggtgatgtg acattgtcag aagatgtgac
    25921 gatctggtat caagctgttt tacgcgggga cagtaattgg ataaaaatag gacaaagaac
    25981 gaatatccaa gatggtacaa tcatccatgt cgatcatgat gcacctgtag atattgcgga
    26041 gaatgtgacg gtaggacatc agtgcatgct acatggctgt acaatcgaaa aaggggcgct
    26101 gatcgggatg gggaccacga tattgaatca tgcggttatt ggtgagaata gtttgattgg
    26161 tgctggatca ttagtaacag aaggaaaagt tattcctcct aatgtcttgg cttttggtcg
    26221 cccagctaaa gtgatccgtc ctttgacaaa agaagaaata caaaaaaaca aagaaaacat
    26281 ccagcattat gtcgagatag gtcggatgca tgcgcaagga aaattcaagg agcaaagata
    26341 ggcagtagca tccataaaat aaaaaaggat ggtcagtttg agttgaccat ccttttttat
    26401 ttggtttaga taagagagct taaaaaagct tcctttgaaa cagattaatt taatgaagaa
    26461 ctgttttcca tgacttcatc gatcaatcca tatgcttttg cttgttcagc tgtcatatag
    26521 ttgtcacgat ctgtatcttt ttcaatcact tcaagcggtt gtccagtgcg ttcagctaaa
    26581 attttgttta agcgttcgcg tgtttgcaaa atatggcgtg ccgcaatttc gatttcagtt
    26641 gcttgccctt gagcgccgcc aagaggttga tggatcatga tctcagcatt tggtaaagca
    26701 aatcgtttac cttttgtacc agctgtcagc aagaagcttc ccattgaagc agccataccc
    26761 ataacaattg tttggacatc tgctttgaca aagttcattg tgtcgtaaat agccatacct
    26821 gctgtaactg aaccaccagg agaattgata taaagataga tatctttttc agaatcttga
    26881 gcgtctaaga aaagcaattg ggcgatgatc gaattagcaa gatcatctgt tacttggccg
    26941 cttaacatga tgatacggtc tttcaataaa cgtgagtaga tatcgtaagc tctttcacct
    27001 cgtgaagatt gttcaatgac tgtaggaatt aaattcataa tagagttcct ccttgatttc
    27061 gtttagaaat agtgtaccat aatttagttt ctaataccta aaatgtagta tacatttttg
    27121 gtcaaaaaag gtcaaataaa aaacttaggc aaattagtcg cataaacccg ctttatggta
    27181 tgataagcag agaaacaaaa caaggaggaa caacagattg atagttggta ttactggcct
    27241 tatcggagtg ctgacaggtg ctgtagttgt ctatatcggc atgatactgc aaatgcgtca
    27301 cttggaaaat aaagaacagc gtagacgtga attggaattg aaagtcaaag aaatcgagac
    27361 gctgaatcaa ttaaataaaa agatcaacga gatcttgcaa aaacggacaa cgctgatgga
    27421 agaatatgtc agctttgatg catttgatga ttgttatatc acgattgacg attttgcgta
    27481 tctccaatca ttttcagcgc aaaatagttt ttatctgcca aattatattt tagacgagct
    27541 atttaagaat atctcccatc gaaaagtgat tttatctcca gatgaaacaa tgaagattgg
    27601 tggatatgct tataaaggcg gacgcgtgat tttagagaat tttacggata gtctgactga
    27661 aatgatcaat gaaaaaaaag ctcagttgaa gactatgacg cgccagccga ttggtttctt
    27721 ctcgaaagat gatgtttgga gctgatattt tgaagcattg ccgttgcaca tgagagcgtg
    27781 gtaatgcttt ctttttttct taatgataca atagaactga acggagtgaa gaaagatgtt
    27841 acaaatctat gcccatcgcg gaagcagcgg cacccaccca gaaaatacgc ttcctgcatt
    27901 tgcagaagct gtacgagtag gtgcagatgg tatcgaacta gatgtccatc taagcaaaga
    27961 tggttatttg atcgttatgc acgacgaaga agtggaccgg acgactaacg gtaaagggct
    28021 gattcgagaa aaaacattgg aagaactcaa aaaattgaat gcagggggca aattcagcaa
    28081 agaatttcca gcagctaaag ttcctgaatt gagagaagtg ttaggactgc tcctccaaaa
    28141 aaattaccga gggatgctaa tgattgaaat taaaactgat cagtatgaat atcccggtat
    28201 cgaagaaaaa ttagccgaaa cattaaaaag tagagattgg ccttttcaac atgcatattg
    28261 cagctttcat ttggattctt tggaaaaaat cgcattactg gaaccggaga tacagctgga
    28321 cttattgatg agtacttctg ataaaaagat ccaactagca gcagaaaccc cttatataga
    28381 aggagtacat ccaaaaaata cttggttgtt cgaacatcca gtccaggcag ctgattttcc
    28441 aaaagcaatt cgtgcatgga cgattaacga tgaagaagaa atggccaaat gtcaagaact
    28501 caaaatagat ggaataataa ctgattttcc ggagaaagct cttcggtgga aacaatcaac
    28561 gaaaagtagg taaaatatgg aaaaagtatt agtgatcgtc ggtccgacag cagtcggtaa
    28621 aacagcgcta agtgtggaac tcgcacaact atttggagga gagatcatca gtggtgattc
    28681 attgcaaatc tataaaaatc tggatatcgg tacagcaaaa atcaaagaag aagagatgaa
    28741 taatgttccg catcatttga ttgatgtgat cgaacctact caacgttact cagccgctga
    28801 ttttcagcat tcaggtcgac aattgattac cgagattgcg aaccgagggc acctacctat
    28861 catagctggt ggtacgggtc tctatatcca atcccttcta tatgattatc agttaggagc
    28921 gacagaagaa gcgaatcctg agattagaaa aaagtatgaa acgttagctg aaacaattgg
    28981 ttcccaagca ttatggaatc agttgaaaga aaaagacccg gcagctgctg agaaaatcca
    29041 ctggaacaat caacgcaaag tgataagagc tctggaagtt ttagagacga caggacatag
    29101 tatcacggca cctaaagaag agccaaagca attgtatgat tacctcatga ttggattaga
    29161 tacgaagcgt agtattcttt atgaacgaat caatcaaaga gtcgatttga tgctagcaga
    29221 aggtttagta gcagaagcgc gtaaggtgta tgagcttgga gaagtccagg ccagtcaagg
    29281 tattggatat aaagaatttt tcccttattt tgaagggaaa gaaacattgg aagaagcaac
    29341 agaacaagtc aaactacatt cgagaagata cgcgaaacgt cagttgacgt ggtttcgtaa
    29401 ccgtttggat gcacagtggt ttgatcttat aagtgagcca aatcaaaaaa gtcagattga
    29461 gcaaacaatc aaaaaatggc tggaggcgga ataaaatgga agaaaaagtg atcatcgtag
    29521 gggtagaaac cgaagcaaac cagcgttact tttcagagtc aatggaagag ctagttcagc
    29581 tgacgaatac cgcatcagga gaagtcgtat tcacaattac acaaaagaga ccacaagtgg
    29641 atcggcaaac gattatcggt aaagggaaat tacaagaatt agttcaacaa gcggatgcgc
    29701 atgaagcgga tttgatcatc tttaatcatg agctaactcc gagacagagc caactgatta
    29761 ccgatgctgt tggtcttccg gtaattgatc gcgtgcagct tatcttagat atttttgcga
    29821 tgcgtgctcg ttcgaaagaa gggaagctgc aggttgaact tgctcagtta gaatacctgc
    29881 tgcctcgttt agtagggcaa gggaaaacat tatctcgtct tggcggtggg atcggaacaa
    29941 gagggccagg tgaaacaaag ttagaaacag accgccgaca tatacggaat aaaatttttg
    30001 ctgttaagaa agaattgaaa gaagtagaag ctcatcgaga gcgcaatcgc caaaaaagaa
    30061 aaaatagcga gatttttcag attggactta ttggctacac gaatgccggg aagtcaacta
    30121 tcttgaatct attgacacaa gcggatactt attcgaaaga tcaattgttt gcaacgttag
    30181 atccattgac taagagatgg cgttttgctg aaggatttga gattactgta acggataccg
    30241 tcgggttcat ccaagacctg cctacgcaac tgatcgatgc tttccattct acattagaag
    30301 aaagtcaaaa tatggatctt ttgctacatg tagtcgatgc tagttctcca gatcgtattt
    30361 tacaggagca gactgtctta aaattgatgg atgaactgaa tatgaaagaa atgccgatac
    30421 tgactgttta caataaggca gaccagatcg acccagcgat gttcacgcct acgctattcc
    30481 caaatgtatt gatttctact cagacgaaag aaggtaagat ggcgttgatc gaagcaatca
    30541 aacgtcagct gatggaacta atgacccctt atacactttc tgtgccaagc aacgaaggac
    30601 agaaattaag cgagttgaga agacaaacaa tggttttgga cgaagcgtac atggaagaaa
    30661 caaacgaata tgaagttaaa gggtttgcaa aaaccaattc aaaatggtta agaaaaaacc
    30721 aataaaattt tattttttta a
//
LOCUS       DAZUMT010000022        30660 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000022 DAZUMT010000000
VERSION     DAZUMT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.022"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..2126)
                     /gene="glyS"
                     /locus_tag="ACG8FM_000917"
     CDS             complement(51..2126)
                     /gene="glyS"
                     /locus_tag="ACG8FM_000917"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS5093598.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2128..3045)
                     /gene="glyQ"
                     /locus_tag="ACG8FM_000918"
     CDS             complement(2128..3045)
                     /gene="glyQ"
                     /locus_tag="ACG8FM_000918"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS5093599.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3429..4241)
                     /gene="recO"
                     /locus_tag="ACG8FM_000919"
     CDS             complement(3429..4241)
                     /gene="recO"
                     /locus_tag="ACG8FM_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS5093600.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4384..5283)
                     /gene="era"
                     /locus_tag="ACG8FM_000920"
     CDS             complement(4384..5283)
                     /gene="era"
                     /locus_tag="ACG8FM_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS5093601.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5298..5693)
                     /locus_tag="ACG8FM_000921"
     CDS             complement(5298..5693)
                     /locus_tag="ACG8FM_000921"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5093602.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5677..6153)
                     /gene="ybeY"
                     /locus_tag="ACG8FM_000922"
     CDS             complement(5677..6153)
                     /gene="ybeY"
                     /locus_tag="ACG8FM_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS5093603.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6170..8362)
                     /locus_tag="ACG8FM_000923"
     CDS             complement(6170..8362)
                     /locus_tag="ACG8FM_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS5093604.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8386..9357)
                     /locus_tag="ACG8FM_000924"
     CDS             complement(8386..9357)
                     /locus_tag="ACG8FM_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS5093605.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10063..11442
                     /gene="brnQ"
                     /locus_tag="ACG8FM_000925"
     CDS             10063..11442
                     /gene="brnQ"
                     /locus_tag="ACG8FM_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5093606.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11598..12044)
                     /locus_tag="ACG8FM_000926"
     CDS             complement(11598..12044)
                     /locus_tag="ACG8FM_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS5093607.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12071..12247)
                     /gene="rpsU"
                     /locus_tag="ACG8FM_000927"
     CDS             complement(12071..12247)
                     /gene="rpsU"
                     /locus_tag="ACG8FM_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS5093608.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12409..12837)
                     /locus_tag="ACG8FM_000928"
     CDS             complement(12409..12837)
                     /locus_tag="ACG8FM_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5093609.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12929..13789
                     /locus_tag="ACG8FM_000929"
     CDS             12929..13789
                     /locus_tag="ACG8FM_000929"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS5093610.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13873..14223)
                     /locus_tag="ACG8FM_000930"
     CDS             complement(13873..14223)
                     /locus_tag="ACG8FM_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5093611.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14239..15966)
                     /locus_tag="ACG8FM_000931"
     CDS             complement(14239..15966)
                     /locus_tag="ACG8FM_000931"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS5093612.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16179..17105)
                     /gene="trxB"
                     /locus_tag="ACG8FM_000932"
     CDS             complement(16179..17105)
                     /gene="trxB"
                     /locus_tag="ACG8FM_000932"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS5093613.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17250..18683)
                     /locus_tag="ACG8FM_000933"
     CDS             complement(17250..18683)
                     /locus_tag="ACG8FM_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5093614.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18683..20086)
                     /locus_tag="ACG8FM_000934"
     CDS             complement(18683..20086)
                     /locus_tag="ACG8FM_000934"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5093615.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20316..21218
                     /locus_tag="ACG8FM_000935"
     CDS             20316..21218
                     /locus_tag="ACG8FM_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5093616.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21259..23145)
                     /locus_tag="ACG8FM_000936"
     CDS             complement(21259..23145)
                     /locus_tag="ACG8FM_000936"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5093617.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23242..24093)
                     /gene="licT"
                     /locus_tag="ACG8FM_000937"
     CDS             complement(23242..24093)
                     /gene="licT"
                     /locus_tag="ACG8FM_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5093618.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24551..26335)
                     /locus_tag="ACG8FM_000938"
     CDS             complement(24551..26335)
                     /locus_tag="ACG8FM_000938"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093619.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26335..28086)
                     /locus_tag="ACG8FM_000939"
     CDS             complement(26335..28086)
                     /locus_tag="ACG8FM_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS5093620.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28228..28452)
                     /locus_tag="ACG8FM_000940"
     CDS             complement(28228..28452)
                     /locus_tag="ACG8FM_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS5093621.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28703..30289
                     /locus_tag="ACG8FM_000941"
     CDS             28703..30289
                     /locus_tag="ACG8FM_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS5093622.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8525 a         6595 c         5485 g        10055 t
ORIGIN      
        1 agaaatagcc tattttgtgc ctgttctctg gtttatttac atattagaaa ttattttgtg
       61 atgatttgat ctaaacttgc tagtgacaaa gccatggcag caatttgact taattgcgtt
      121 aatcggttct tacgtactgc tggttcttct gccatgacca tcgtctgatc aaaataattt
      181 tcgatcagtg ggcgaaggct tacaagctga tcataatttt cgttgatcgt ttggtcagca
      241 aagcttgttt tcattgcctc tactgctttg tgcagttctt tctccgcttc gttttcgaac
      301 agttcagggt ctacttctga ttgtttttca agctccgctt ttttcgctaa gttcacgaca
      361 cgtgtcaaag cttccatcga tggtttgaag tcttcatctg caagatgctg cttgaacaat
      421 tgtgctgctg caaatacttt tgtcagatct ttttgttctg cattcaaaac agcttcgatg
      481 acatcatgac ggatattctt cgttgtcaat aattggcgca gacggccttt gataaagtcg
      541 atcacctgtt gttgtcccgc tgacaagcca atgccaaaac gatccgtatc tttactgata
      601 acttcactaa tcgtttcttg cagaacagac aaaggaaagg cccatttttt cgattcgatg
      661 attcgaacga caccatatgc ttgacggcgc aaagcatatg gatcgtttga gccacttggg
      721 atcatcccaa ctgcaaagaa agaaaagata ctatctaatt tatcagcaat cgctaaaaca
      781 gaacctactg tagtctcagg taaatctcct tcactggata taggcatgta atgttcacgg
      841 atcgctgttg ctacggctgg tttctcgcct ttcagcagag catatttttc tcccatgatc
      901 ccttgtagtt cagggaattc tcctaccatg ttggttacta gatcaaattt atagatttca
      961 gcagcgcgtt gcagatccat caattcttcc tcagatagtc ctacttgttc gcctatgatg
     1021 cgagcgatga cttgtacccg ttgcattttc tcatagatgc taccgatttt ttcatggaaa
     1081 gtaacagttt ttaatttttc aacgtattgg tcgattgaag ctttcttgtc ttcattataa
     1141 aagaattccg catcttctaa acgagccgtc aatacttttt gattgcctcg taccacattt
     1201 tccaatttta cccgatcgcc attacgagca gaaataaaat gaggaagcaa cataccttga
     1261 tcgttccgta cttcgaaata acgttgatgt tctttcatag aagtgactag tacttcttca
     1321 ggaacagata gatatttttc ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa
     1381 ttgttgactt cttctagtaa atcttcatct agatcaatga tccagtgatt tttttcggct
     1441 aattcgtttg cttgtgtgat gatcatttct tttctttttt gcggtgaagc aataacaaat
     1501 tgttcattca atttttcttc gtattctttt gcatgcgtaa atgtcacatc ttcgcctaag
     1561 aaacgatgac ctcttgaagc tctgtctgtt ttgacatcca agatctcaaa cgggattatt
     1621 tcgtcatcta acaatgccac gatccagtga atagggcgaa tatattcaaa atcgtattct
     1681 gcccagtgca tcgttactgg gaaagtgagg cttttaatga catctgctaa acctgtcaat
     1741 acttcttttg cttctttgcc gtgagtgtat ttcgttacat agacatattc cacgcctttt
     1801 aattctctaa aagtgatagc atctgtcgtc agaccttgtc cgcggacaaa tccttcagct
     1861 gctttcgtcc agtttccatc tgcgtccaaa gcaatttttt tggctggtcc tttgacttct
     1921 tcttcgctgt ctgcttgacg ttctggaatg tttgttactt tgattgctaa gcggcgcggt
     1981 gtagaaaacg tttcgatcgt atcataagtc aattggtttt catctaaaaa ttttgctgtt
     2041 ttttcttcta attgtttcat gcttggtgtg acaacatgag caggcatttc ttccagcccg
     2101 atttcaagta ataagtcttt tgccatctta ttcagcctcc ttcatgtttt ctggatttgc
     2161 tgtgttttta ttcaataaag ggaatcctaa tttttcgcgt tctgccacaa agattttggc
     2221 tacagcacga gccatgttgc ggatacggga aagataacct gctcgttctg ttacggatac
     2281 tgcaccgcga gcatctaata aattgaatgt atggctgcat ttcaaaatat aatcatacgc
     2341 tggatgaaca agattttcat cgatacagcg tttagcttct ttttcaaaac ggtcaaagtt
     2401 gcttagtagc aagtcttggt cacttacttc aaaagaatat ttggaatgtt catattctgg
     2461 ttgtttgaag atctcgccat atttcacacc atcagcccat tccagatcat agacactttc
     2521 aacttcttga atatatgaag caagacgttc tagtccataa gtgatctcag aagtaactgg
     2581 tttacaaggt agtcctccga cttgctggaa gtacgtgaat tgtgtgatct ccatcccatc
     2641 aagccatact tcccaaccca caccggcaca ccccatcgat gggttctccc agttgtcttc
     2701 aacaaaacga atatcgtgtt ctaatggatc aattcctaaa agacgcaagc tttctaaata
     2761 aagctcttgg atattttcag gtgaaggttt cattacgact tgaaattgat gatgctgata
     2821 caaacggttt gggttttctc cataacgccc gtcagctggt cggcgggatg gctctacata
     2881 agcagcattc catggttccg gtccgattgc ccgtaagaat gtatatgggc tcatcgtacc
     2941 tgctcctttt tctgtgtcat aagcttgcat cagcatgcag ccgtttgatg accaaaattt
     3001 ttgtaatgtt aaaatcatat cttggacggt caatttttta ctcatctgtt ttgccccttt
     3061 caatttttaa gcgttgctgt caataagaat acaggaaaga aaacgacaaa aagtccctat
     3121 gtctgattac agacataggg acgaataatt tcgcggttcc accctacttc cgaataaaga
     3181 ttcggcagct tcatttatcg tactcgcaag tgcctcttca aaaatcgttt gggtccggct
     3241 tccactctcc cgaactcgct aaaccacgtg ttttttactg atcttggtca atgtacgtat
     3301 tcattttgca gttttcttgt ttatataata gatcctctca gtgaacgatc tatttaatct
     3361 acggttaata atagacggaa gtccctgtat ttgtcaaata gttttgattt ttttgttgta
     3421 tctttcattt attcttcttt ttcaggtatc tctttttcag gcatattttt ctctggcgtc
     3481 tttaatacgt gtgcccaaga cttcatttga tcaatgaact ttttgctttt tagatgaata
     3541 ccgacatatt catcatacaa ctggtcaatg aggtcacgga ttgcctgctt cgtttcttct
     3601 ttcaagtgga ttccactgat cttatcataa gaaatagccg aaaacattcg aacgaaataa
     3661 acagcgcgag gatctgcatg ataacggtgt tcgtccattg gccagtgttt ctcgcatatc
     3721 acaccgccag atttggaaga atagtcaaat tttccttttg tttccccgca tacgacacag
     3781 tgcgtccaaa ttggcgcgat tccgaagcgt tgcagtattt gaacttcaaa gatattcgtg
     3841 atgatttctg catcatctcc ttgatccatc cgctgcagag cttgttgcgt aaattggaat
     3901 aaatgcgggt catattgatg atcttcgatt gctgcatcta ctagatttag caaataggta
     3961 ccataaccgc tcaaaaagat atcttcttgg atgttccgaa acgcatggac gtctttgaca
     4021 ctgcttaaaa acgacaaacc ttcacttcga aaattaccga tatagactgc ttcggtaaag
     4081 gggagcaaag ccggagctaa tggctgattt tgccgatgga ttcctttgat ataaaacatc
     4141 attttgccag aagattccgt aaagattttg actaatttat ctttttcttt gtaattacgg
     4201 ctagataaaa tcaagccttt cgattctgca agctggctca tattctccct cctaaaaaat
     4261 aaatctggaa aagcagattc tgccgatcca gattatttat gtttcgatct tccgccgttt
     4321 ttctcaacgt gcggattttt atccagttta tttctatact taacctataa gaagttcaga
     4381 atatcagtat tcgtcttttc ggtagccaaa gtcttgcaga tagacttttt tgtctcgcca
     4441 gtctttttgg actttgaccc aaagttctag aaatactttg tctcctaaaa gattttcgat
     4501 atcctttctt gctttcgttc ctacttgctt cagcattttt ccgcctttgc cgataatgat
     4561 tcctttttgg ctatctcttt cgacgatgat cgtggcctgg atatggattt tatcattctc
     4621 gtttcgcttc atcgaatcaa caacgactgc gacagaatga ggcacttcgt ctcgtgttaa
     4681 aaacaagact ttctcacgga tcaattcaga aacgataaag tattcaggat gatcagtcac
     4741 ttgatcatct ggaaaatatt gtgggccctc tggcatttca ctgactaata catccattaa
     4801 gcgttcgaca ttgtttcctt ctgtagcaga gatcggaatc acttgtgcaa aaggcatctg
     4861 cgtcgaataa tcttcgatga ttcccagcaa ctcgtctgga tggatcttat cgattttatt
     4921 gataatcaga taaactggag aatgcacgtt ttttagacgt tcaataataa aatcgtctcc
     4981 ccggccgcgt ttttgatcag cactgatcat aaataagact gcatccactt ctttcaacgc
     5041 actgtaagct gcttctacca taaaatctcc tagacgatgc tttggtttat ggattcctgg
     5101 tgtatcgata aaaatcagct gagcttctgg tgtagtatat actccttgga ttttatttct
     5161 agttgtctgt gccttgtcac tcataatggc aattttttga cccacgattc ggttcaataa
     5221 tgtggatttt cctacattcg ggcggccgac gattgcgaca aaccctgatt tatgttctgg
     5281 catatacgtt ctccttttta attaaacaac aaatgatata ttttagggac aaataaaatc
     5341 aacccaatga tgacagcaaa acaagcggtc actaaaactg ctccagctgc catatctttg
     5401 atttttttcc caatcggatg aaaatgaaaa tccgtaaaca tgtccactac attttcaaaa
     5461 acagtattga tgatctctac tatccaaacg agaaaaacag ctaaaagcag ccataaccac
     5521 tctatccgat caagctggaa aatcacgccc ataaataaag caaggatacc gaacgcaaca
     5581 tgtttcctca tattgcgttc ttcctcaaaa acggttttga ctccttggac agcaaattcc
     5641 acagacatca ggaaatgttt gtttttctct acgtttttat cttttgagtc cataggcatc
     5701 caagatctct ttctgtaaac caaacatttc tttctcatct tctggtgtca tatggtcata
     5761 cccgttgata tgcaagaatc catgtaccgc taaaaagcca agttcccgat catatgaatg
     5821 gccgtaagct tctgcttgtt cagcagcacg ttcaatagaa atcatgatgt ctcctagtac
     5881 acgaggcaaa ggattctctc catcttcaaa aatcactggg atttcgtctt cgccttcttc
     5941 ttccagagca aaactgatga cgtctgtcgg tgcatctttt ccacggtaat cacgattgat
     6001 tacttggatt gccgcattgt ccatgaaagt aacagacatt tctgtttcct ctggcagctt
     6061 taaataagat gctgcaaatt gcagcaagtc atcgatttct ttcattttct cttctgagac
     6121 ttggtttgtt tcatccataa aggtaatatc cattatacat cgcttctttc tattcttctt
     6181 gttcttcctt tatcttctca acttctgttt tcattttagg atatttgatc cgagaatgga
     6241 aggtgctact taaaccgctg atcaatgatt tttccacttt tcggatttct ttcaaggtca
     6301 gtccactatc gtctaattga ccatcggaaa tacgctcttc gataagatta tggacaaaac
     6361 tttcgatttt ttcgctcgtc ggatgatcca ttgcccgtac tgcagcctca caactgtcag
     6421 caatattgac gatccctgct tcacgacttt gcggttttgg tccaggataa cggaattgct
     6481 cttctgttac ttccggattg cgttctttcg ctttgatgta gaaataacgc attagcgtgg
     6541 ttccatgatg ctgtttgcaa atatcgatca ccatttgcgg catattataa tcttccaata
     6601 ttttggctcc atctgtcaca tgtccaaaga taatatgttt actgtcttct ggcaataaga
     6661 aattgtgcgg gttttccgct cctgaaggca agttctcaac gaaaaagcta gcatgtttga
     6721 tcttgccgat atcatgataa tagcaagcta ctcgagttag caaagaacgt ccgccgattt
     6781 ctgctacagc gttagcactt agattagcta ccatcatact atgatgataa gtacctggcg
     6841 cttcttccag caactgtttc aataaaggat gatttggatt gctgagttca tttaatacaa
     6901 ttacactgtc atcattgacc atcaattcaa tatatgggtg tagaccgatc gtcagcaaga
     6961 atgagaagag tgcaccagtg aacccgcaga caagcattaa ccaagtcgtt gcatcaccaa
     7021 agctcattcc ttgatagacg atcaaaatga catccatcat cacaggaaaa ataatgaccc
     7081 acatggcagc agataatcct tgttctgaga tccgtttgcg tctgacaatc gttgccatga
     7141 gcccagagaa aacataagac atcaagataa tcgttagaaa gttcgtgcca atcgaatcat
     7201 aaaaaatgaa aagtgcagat actgcttgga aaataccagc catgatccct gctcttcggt
     7261 tcaagaaaaa gcttaaaaca agtggtgcaa aagcagcggg ataaaacagc gggatataag
     7321 tagcttgttc cgtttggaat aattggaaaa atttcatcag cactatgctg actgacatag
     7381 ctgtcacata gaaaagcacg tattttgttc tttctcctgc attttggaat tgaagtgaat
     7441 tataaatcag tacgccgatt tgaagcaaaa cagctaaaac catggcaatg agtgggaaaa
     7501 tcgaagtcgt ttgattcgtc aatcctagta attccaattt tttcatcgct gaagcatcaa
     7561 tttgatttcc ttcgcgaaca atgatttctc cttggaagat catcactggt tgtacagctt
     7621 ctcgtgcaga ttgttttaat tcttctgtct ttttctcatt caatacatca ttgactacaa
     7681 tgccttgatc cactaagtag cgaatcattt gttgctgctc tgttgtgaca ttcaaatact
     7741 gaatctgatc ttctgcttct tgtttaaaag cttccaattc gctttcacga acttgtttac
     7801 tcatctgttc gtcaatgagc ttcaaacttt catccctaac cgtgtgaatt tccgtttcag
     7861 tcatttcaaa tatcgtcgaa taaaaattgt tcggcagttt ttggtaaaac gcaacgtttt
     7921 ctgcattcac gttttcaaaa tttttcttca gtgtagcaat ccgctcttct gtagtaggtt
     7981 tcgggatatt ttcatcttcc ttcgccttat ctaccttgtc attataagat ttgttgatgg
     8041 tatcattcgt cttatcgatc aattcgaata attgcttgat ccgattgttc tggtcatctg
     8101 ccagatcttt ttgataagta tattctggag tcactgcttc tgctgccagt tttcttcttt
     8161 gttcggtttc ttccgtgttc tcgatcgttt tattcgcacg gatactctct tctgctactt
     8221 ggccttctct gaaatcattg gccttttgtt tgacactact aaatgtaacg ccgcagacaa
     8281 tgataaaaaa caagaacaga aggaaaggca atgctgcctt tccgagtttt tcttgtagtt
     8341 tctgcagtgt tttattcaac atgtgcttat cctcctaagc aaagcttatt cttgatgaag
     8401 atccgccttt tcataagctt tgatgatttc ggcaactaca gggtgccgaa caacgtcact
     8461 tgcttcaaaa ttaacaaaat cgattttttt gatttgcttt agtgttcgtt ctgcatgaac
     8521 aagaccactt gtcgtccctc gaggcaagtc tatctgactt gtatccccat tgacaatcat
     8581 ttttgattga taaccgagac gggtcaagaa cattttcatc tgagcgactg tcgtgttttg
     8641 tgcttcgtcc aagataacga atgcatcatc caatgtccgc ccccgcatgt aagctaatgg
     8701 tgcgatttcg attacgcctc gttccatcaa tcggttcgta tgatccaatc caaaaatttg
     8761 gtataaagca tcataaacag gccgcagata aggatccact ttttctttca gatccccggg
     8821 tagaaagcct aggttttccc ctgcctctac agcgggccgc gtcaaaatga ttttttgcac
     8881 ttcgcctttt tttagggcag caattgctaa tactacggca agaaacgttt tccctgttcc
     8941 ggctggccct acaccaaata cgatgtcatg tttggctacc gattcaacgt atttcttttg
     9001 accgctgttt tttacccgta taggttttcc attacggtct ttgacgatct cttcctcata
     9061 catctcaaca aaataatcta atgtaccttt ttgccccatt ttcaatgccg tcacgacatc
     9121 tggtgtactt acgtggattc ctcgtttgat caattcctgt aatgtccgaa tgactgaggc
     9181 agccaaggaa atctgggatt gttcaccaat gatctgaatg atttctccac gtgtatggat
     9241 ggttgtttcc gttgtttcct cgatcagttt tatatgcttg tcgtgtgcac ctaaaagcat
     9301 gctgacatca tcagcagctg tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa
     9361 tagacatccc ctttttttcg attcattctt tcctatttta ccatactcag aagaaactat
     9421 atagcaaata agaaaaggtt gtaaaagagt cgtttaacca tacgtaccaa gaaacgttaa
     9481 aaaatagctg tttccattcg ctaaaggtct cgttttgata aaaagaatct gagaatgtaa
     9541 gttatatgaa agtggagaac aaaatattcc gtttcattca gaggtattct ctaggtattt
     9601 ttcttgtttc aaaaaaagaa aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca
     9661 ggtgtattat tacgtcaaca aataaacata cagcaaagcg tcgaggaaaa cagtaggcta
     9721 ttcaacgatt tattcccagt gagtcatgta agtgagaaat gacaataaat cgaatagacg
     9781 aacatcattt ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc cattatcagg
     9841 ctagcggata actttatcaa aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa
     9901 ggtggtaccg ttctagaatc tagagccctt ttcccaagtg gaaaagggct tttttttata
     9961 ttttaaggtg gtggttttca tggaatctga tattagtggc attactaatt gctgcaacgt
    10021 ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga aacaaaaact
    10081 tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt tttcgggtca
    10141 ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcgcaaactg ggtcagtgct
    10201 ggcgtaggat ttgccatctc aggcgctctt ttcccattac tagctatctt agctgtagta
    10261 gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg gtatgctgcc
    10321 ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc acggacagct
    10381 gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa ttcattaggt
    10441 atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt ccatcccaca
    10501 cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt attagcaatc
    10561 atttttgtcg tttctttatt ccacccaatg ggaaatctaa atcaatctgc tgcagatact
    10621 taccaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt tgatgcagta
    10681 gccttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg tttcaaagat
    10741 aaaaaagtca cagaattaac agcaaaagct ggtagcctga gcgttgcgct agaagtcatg
    10801 atctattttg gtcttgttct tctcggtgcg atgagtttga ataagatgtc tttatcagaa
    10861 aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt cggcgctagc
    10921 ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact cgttgtttct
    10981 tttgcacaag atttccataa actgttccct aaagtcagtt atatgacatg gctgcggtta
    11041 acgactttcg tctcttttgt cgttgcaaat gctggtttgg acaatatcat ccaatggtct
    11101 ttaccagtat tgatgctttt gtatccattg tcgcttgcgt tgattttgct ttccttaaca
    11161 gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt tgctgctgtt
    11221 cccgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca aagagttgta
    11281 gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattggg acttggctgg
    11341 atggtcccaa cgttacttgg gtatgctggc agcttactct tctattacgc ctatcgccta
    11401 tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccagatca aacacaattt
    11461 aacataagaa atattaaaca ggaaaaaaac agctagatga aagaatccta ttctttcgtc
    11521 tagctatttt ttagatctct tttttacgag cacaaaaaaa ctgcggacaa tgattgtccg
    11581 cagtttaaat gataaaatta agattgtaat agttctttca caatggcatt tacttggttt
    11641 ccatctgctt ttcctttgac tttaggcatg atcgcaccca tgactttgcc aaattctttc
    11701 attgatgaag caccagtttg cgtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg
    11761 gaaagttgtt ccggcatgta tttttcgact atcacaattt cactttttac tttttcagct
    11821 aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg
    11881 cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg
    11941 ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc tttcgctttc
    12001 atcgctgttt tcatgtcatc gtttaatcta cttagtagtg acacactacc aactccaatt
    12061 catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc ttgcgtctta
    12121 cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc gcttttgaca
    12181 cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga acgactgttt
    12241 ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt attgaaatat
    12301 atctcaaaaa acaagactgt cctgtaaata ttatatctaa tgctgttttg accgtcaagg
    12361 aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt attgacaatt
    12421 ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtacatccac cgagctgttc
    12481 ttcgaaataa ttcattggac acatatggat ttctttcgtt gttccgcaca ccgtacagat
    12541 aaagtgatgg tgatggccga tttccccttg acagcaatga aaacgaaact tcatctctcc
    12601 atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat ttcgataaat
    12661 cgtatcgtaa cttactcctt tgaattcctg attcatatag tcaaaaactt ctttagcgga
    12721 tacgtagcga ttgcgcttca ctaaataagt taacaatgct tctctttttt tagtatattt
    12781 aaaaccgtta tttttcaatg tttctagtga ttgttcgatc aacgtctgtt ttttcatgac
    12841 ttattcctcc aaatcaaaat gattacgaag tacacattct agagtatggt ataataaaca
    12901 taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa tcgttacttt
    12961 ttttgtcatc tccgactctg ctggagaaac tgcgacaaaa ctagcgcaag ccactatggc
    13021 ccaatatcct agcgttgagt tcaatttatt ccgcagaaca tttgtgacag atgagaaaac
    13081 attgatgcag gcactgcaag atgcactaga agaaaaagct cttgtcctgc acacattgat
    13141 caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat tatttagttt
    13201 tgatgtgatg actccgccga tatcagaaat cgagcgctta actggtatca agcccattcg
    13261 gcaacctggc gcccttcatt tattgaatga gaactatttc aaacggatca aagcaatgga
    13321 atttgctgta aaatacgatg acgggaaaga tcctcgtggg tttctagaag cagatgtcgt
    13381 tttgctaggc gtatcccgaa cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa
    13441 tttaaaagtt gccaatctac ctttgatccc acaagcacat attcccaaac agctttggga
    13501 gatcgatcct aaaaaaatcg tgggactgac gaataaccca gatattttaa acaatatccg
    13561 taaagaacgg atgcgctctt acggattaaa tcctgatact gcttattccg atatcgaaaa
    13621 aatacgagct gaactcgatt ttgcgaatga tctttatgaa aaattgggct gcgtagtaat
    13681 caatgtcgct tctttatcta ttgaagaaac agcttcgctt attttgaatg ctttagacct
    13741 agaagaccat agttattatg gaactgaaac caccgaagaa acagactaac aatttttctt
    13801 taaaaaaact ggactgtgtc agcgaagaag attcgttgac acagtccagt ttgtctctat
    13861 ttgcacctat cgttattgat gttccacatg tttgagtgtc tggttcaaaa tatccagtac
    13921 gtgagaatca tcaagactat acaaaatcgc ttttccctca cgatgggact tgacgacatg
    13981 gttttctctt aataaacgca gttgatgcga tacagcagat tgttccattt ccactgcttg
    14041 gatgattgct gtcacatttt gttccccgtc tttcagatac agcagaattt tgagacgagt
    14101 ggggtcactc aacattttga atagttggct gactttctct acttttgttt tttgcgtgtc
    14161 tggcaatcca ttcacctcca acttgaaaaa agactgactt ctaaagttta ccactttttc
    14221 catcagtctc aagttttttt attcttctgt catcttttgg ataatcgttt cataagcggc
    14281 tactttttca gctgcgtctt ctgaagagtt tccttttaca ccgatataaa atttgatttt
    14341 cggctcagtt cctgaaggac ggatagcgat ccagccattt tcttccagcg tatacttcaa
    14401 tacgtcagat ggcggtgtcg ttagtttttc tacgtttcct tcacggtctg ttctcgtcaa
    14461 ctctttgaag tcttctgttt ggatgacttt tgctccggca aattctgttg gtgcttcttc
    14521 acggaatttt tccatcaaag cagagatttt cgcagaacct tcttgtccgc tcattgtcac
    14581 agaaatcgtt ttttctgcgt agtagccata ggtttcaaaa atcgtttgca agccatcata
    14641 caacgttttg ccttgttttt tatagtaagc tgctacttct gctaataaga gcaatgcttg
    14701 gatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa ccgtaacttt cttcgaatcc
    14761 gaacataaaa gtatgggaat gatcttcttc aaattgttga attttttcag caatgaattt
    14821 gaatcctgtc aatacattga acatggtagc cccatagtgt tccgcaattg ctgtaggtaa
    14881 ttcgctagaa acgatcgatt tcaataccgc tgcgttttca ggtaaagtgc ctcgcgattg
    14941 gtgtgcttcc aaaatatatt gagcaaacag tgctccgatt tggtttccgg tcaatacttc
    15001 ataggagcca tttggcaaac gaacggctgc acctaaacgg tctgcatcag gatctgttgc
    15061 aatcagcaaa tcagcgcctt ctttctcgcc aagtcgtatc gcatactcaa aagcagagtg
    15121 ttcttctgga tttggtgatt tgactgtagt gaaatctggg tctgcgacag cttgttctgg
    15181 aactagtacg aattgttcga atccagcttg tttcaacgca cgttcgccca acatttttcc
    15241 tgttccgtga agtggtgtgt acacaagttt caaatctttc cccattgttt ccaccaattc
    15301 atgatcaatc gttactgctt tgatttcttc tagataagcg gcatccactt cttctccgat
    15361 gatcgtgatc aatccgctat gttcagcttc ttcgtcagat aatacttcaa cttttaacgg
    15421 attgctgact tcacggacat atttcgtcaa tgcatctgcg tctgcaggag gcatttgtcc
    15481 cccatcttct ccgtagactt tatatccgtt gtaagctgct ggattgtggg atgcggttac
    15541 catgattccc gcaaacgtct tcaaatgacg gactgcaaaa gaaagctctg gtgttggacg
    15601 tagactttcg aatacataag atgggatatt gtgatgtgct aatgttttgg ctgcttccat
    15661 ggcaaattct ggtgattgat ggcgtgagtc ataagcgata gcgacacctc ggcgttttgt
    15721 ttcttcgcct tgtgtatcca tgaaacgtgc aagtccttct gttgcttgtc ttaccgtata
    15781 gatgttcatt cgattgatcc caggccctaa gattccccgc atgcctgcag taccgaattc
    15841 caacggtgca taaaaagcat cttcaagttt ttcaggatct tcacccagat catttaattg
    15901 tttttttaag ttttcttcta aattttcttc atgtacccat tgttcataca cttgttccca
    15961 agacatgaaa ataacacctc tttctgaaat atggttctta ataagtatag cattcactta
    16021 ttagatatga aagtatgagg accaaaaaac gcttaaatca agaaaggatt cttttatcaa
    16081 gcacggttgc aagtattcgc ataaatcgga ttcagtagat ttcacgcgaa aaaaaagagt
    16141 aaaagaacaa ttaaaccgtt cttccactct ttttaaactt atgcagttgt ttcttctaaa
    16201 gattccaagt atttatacac ttgttgtcca gcgatcccac catcaccaac agcagtagtg
    16261 atttgtcgca aatctttttc acgaacatct ccgatggcaa aaacgcctgg tatattcgtc
    16321 ttcatttcgt tgtctgttgg aatccagcca gcttcgttcg tcaaccctgc ttctctaaat
    16381 ggtgcagtta atggatccag tccaacatag atgaatactc cgtctgctgc caaatcaaac
    16441 tcattgccgg ttttgacatt acgtgctttc acacctgtca ctaccatttc attcccaacg
    16501 atttcgtcaa cgactgtatc ccacataaag gaaatttttt cattcgcaaa tgcacggtct
    16561 tggatgatct tttgtgcacg taattcatct ctacggtgga cgatgactac ttcagaagca
    16621 aattgtgtca aatagatggc ttcttctact gctgagtctc ctcctccgat cactactaga
    16681 cgtttgttac ggaaaaacgc accatcacaa actgcacagt aagaaacgcc acgtccagca
    16741 aattcttcct cgccaggtac gcctaattta cggtggatac agcctgttgc gataatgact
    16801 gtttttcctt cgtaagtttt atcttcacaa ataacttctt tgtggctgcc gtgatccttg
    16861 atatcttgca cgattccata agcattttct gtcccaaatc ttgaaacacc ttcatacatc
    16921 ttataagcta gatcaggacc cattattgaa tcgaaaccag gatagttttc gatttctgcc
    16981 gtattgttca tttggccgcc aggggcgcct tgttcgatca tcaatacgga taagtttgaa
    17041 cgtgaagcat ataaagcagc agtcatccca gcaggacctg cgccaataac gattacatca
    17101 tacatatgta gattctcctt tacttaatca agtaagcata ctttacagcc aatgcgaaaa
    17161 aaagtctatt gttctgcttg cgtttctcga atagctttat tatactcgaa aaatccgctg
    17221 aaaaacaata gacttttata aatcattgat caatcattcg aaagattttc tccatttgaa
    17281 tcaattactt ttttatacca gttgaatgat ttcttcttcg aacgggctaa tgtgccattt
    17341 ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt tcttcatctc acctgtacca
    17401 gctgatacta aatcaataca tccccaagtc gtatagccta atagatcgac tccatctaat
    17461 tctactgcat ctttcattgc ctggatatgt gctgccagat agtcgattcg atagtcatct
    17521 tccacataac cattctcatc gggcgtatcg actgcaccta agccgttttc tacaataaat
    17581 agtggttttt gataacgatc ataaatatca ttcatcgtta ttctcaagcc taatggatca
    17641 atttgccaac cccattcact tgcttccaga tatggatttt tgacagaagc aaagatattt
    17701 cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa ctcgagaaga ataataagaa
    17761 aaggagataa aatcaacagt atgttctttc agcaatgcgg catccccagg ttccattttg
    17821 atctgaatgc catttcgttc catttctttc aatgcataag caggatattc tcctcgtgac
    17881 tggacatcaa tgaagaaata attttctcga tctgattttc tagaagccca aacatcttca
    17941 ggattgcaag tgtatggata atatgctcct gctgccagca tacagcctac tttgttctcc
    18001 ggatctactt catgagcaat cttcgttgcg atggcacttg caaccaactc atgatgtgcc
    18061 gcttgatatt tgatttgttc ttcattttct ccttcttcaa aatacagccc tgcgcccatg
    18121 aatggtgcat gaaggatcat attgatctca ttgaatgtca accaatattt gacaagtcct
    18181 ttatagcgat tgaagatgac tcggcacaga ttttcataaa aaccaacgag ttctctggaa
    18241 cgccacgcac catattcttt gatcaaatgc atcggacaat caaaatgtgt aatcgtcaca
    18301 agcggctcga tcccgtactt ttgacattct ttaaagacat cctcataaaa tttcaaacca
    18361 gattcatttg gctctttttc atcgcccatt ggaaaaatac gactccaagc aatggacatc
    18421 cgataagtct tgaaccccat ttctgcaaat aaagcaatat cttccttata atgatgatac
    18481 atatcgatcg caatttttgc tggataaaaa tgattttcat caaattcaaa cattttcttt
    18541 tttccagtaa tgacaggaaa acggtcttct ccgattggca caacatctac gttcgctaac
    18601 ccacgtccgc cttcatcata accgccctcg cattgattgg ctgctgtagc tccgccccat
    18661 aaaaaatctt ttctgaatcc catcatactt cctccattac atgtgtattg cttgataatt
    18721 ctcttgccag ttcatcttgg acttcgattc cttctgcttt taaccggctg attgtttcct
    18781 tgaattgcgg taaatactgc tgatgtgcaa taaacaactc attcatgatc cgttttgctg
    18841 tctttcctga tcgtataagc ggattgatcg taaacgcttg taaagctgtt ccataatcac
    18901 cagtcacagc tgcttcgatc gtcagcaatt ccatattctt cattacttgc aaccagcctc
    18961 tttcagcagc aggaagggaa ccaaacgcta catttcgtgc gccttgtgcg ccaacatagg
    19021 cagtgacttc tactacacaa tctgccggca gatcaggaac agctccatca ttctttgtag
    19081 ataccacgat ctgtgtttct ttatttccat agatcgaagc aatcgtttca caggcagcat
    19141 ctgaataata agcaccacct cgtttttgca attgttcagg tttatgatcc aattctggat
    19201 ctttgtataa ttcaaacagc tctgcttctg tttctttcac ttgctgcgct cttgtaccaa
    19261 tcgtttgata ttcttctaaa gcatgttcca gcatttcttc ttcacggtag taatagcggt
    19321 ggtagccaca agggatcatg ttcatttgtt ttaattgttc tttgaaaaac gggatatcaa
    19381 agatattttt cggcaatcca ttgtcttcat cgtataaacg atcaatcaat tctgccgtgc
    19441 ggtcatttcc tttattatct gctactttgt gccaatggaa gtgatttaga ccggcaaact
    19501 tgtagatcag ctcttctttt ttcttgccaa gcattgttgg ttctgtcatc atcgccatta
    19561 ctggcacatt gcataatcct atcactttat cccatttgcc atatctgata actgcttctg
    19621 tcaccatccc gcttgggttc gcgaaattga tcagccaagc atttggacat aaacgcttca
    19681 tgtcttcaac gattgccaaa accactggaa cggtacgaaa agctttgaat atcccgccag
    19741 caccatttgt ttcttgccca atcatcccat aataactagg gatccgctcg tctttgattc
    19801 gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa atccgcatct ttcaatgctt
    19861 cttcccgatc cagagttgca tagattttga cttcatacgg agaagcatcc cacatacgct
    19921 gagacatctg actgacgatc tcaagctttt cttttccttc ttctacatct acgagccaga
    19981 tttctcgtat cggtaattcc tcatatcttt tgataaatcc ttccatcaac tcgggggtgt
    20041 aactgctgcc tccaccaatc gtagcgattt tgattccttt tgccatgttc tcactccttt
    20101 actttttaca cttttattat cttttttgta aacaaaacaa acaatagaaa ttcgtttctt
    20161 tccagtaaca tttacaaaag ttagtgtttt ctactggatt tttgtaaata tcttgtcaaa
    20221 aaatgtcttt attaagctat ataacataaa tccgtttaca aatcattttc aaaaaagtta
    20281 taatttacgt aaaaaaacaa gaagaaggta tctacatgtt attacgtgaa aaaatcaaag
    20341 aaatctcctt ttctccttca gaaaatgaag tgattcagtt tctcttaagc gattctccag
    20401 cacttggcga aatgaccatc caagagattg ctgagcgatg ttatgtccac ccgtccacac
    20461 tcatccgtgt agcaaaaaaa atgggttttg atggctgggt cagttttcgt aatgcttttt
    20521 tagctgaaac agcctatctt gaagggaatt ttcaagacgt agatgcgaat cttccttttt
    20581 ccaccacaga cggtattatg acgatcgcaa ataaaatggc ttctttagaa cagatgacga
    20641 ttcaagatac actttctctc attcatcatg accaactgca aaaagccaaa caactaatgc
    20701 ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa tactttgatt tctcaagatt
    20761 tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat tgtcaccact tttggtgaat
    20821 cgttctatga agcgtacaat tgcacgagtg atatgtgcgg gattctgatt tcttatacgg
    20881 gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa acaaaatcat gttccaattt
    20941 tagctttgac cagtatcggt gattcaacgc tatcttcaat gagtacagcc gctttgcgac
    21001 ttacgacacg cgaacgactg tattcgaaaa ttggtaattt tacgatcaat acatcgattt
    21061 gttatcttct agacgtgtta tatagttgtg tttttgcaga aaattatcaa cagaatttag
    21121 tccatctaat ttctgtagga caaaaatttg ataaacgccc tatcagctca gccatcatga
    21181 aagagccttt caattttgaa aataaagaag tggactaaaa agaataatta agcctagtcc
    21241 attctttcac ctaaccgttc aggtcagtgc ggtgataagt gcatcgtctg gcccgaccgt
    21301 ctccccttga ttttccaaaa tatccaaata atccgtgtaa ttggtgacga tcacaggtgt
    21361 ttctaaactg tatccttcct cttcgatttt ctttttgtcg aagcggagca acagatcgcc
    21421 ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa tatttccctt ccaactgaac
    21481 agtatccaat ccgacatgaa tcagcaattc cagacctgct tcagaaacca ttccgattgc
    21541 atgtttcgtc gggaacaggg tcatgactgt tccgtcaaaa ggggcacgta cttctccttt
    21601 ttcagggtag atcaagattc ctcgacctag tgttccttgt gcaaaagctg catcctttgc
    21661 tgcattcagt ggagcgattt gtccttgtat cggtgttttt acaatttctt tacctgatct
    21721 tttctgtcta tcatctgttt cacctgcttc ctccttccaa aagaaaaaag tcagcgcaaa
    21781 ggataccacc atcgcaatgg cgattccaat caatacatta atcacatccg ttaaatcacc
    21841 atctggtcta atatagttgg gaattgtaaa gatgcctaat ccacctggat tccaagaagt
    21901 taaattcatt aatccactat acgcgccccc taacgcacca ccgacacagg taaaaataaa
    21961 tattttttta cgtggcaatg taaacccata aatagctggc tctgtaatcc ccacaatacc
    22021 agaaacaatg gctggtattg cgattgcttt gtcttttggg tttctaagct taaagtagag
    22081 tgcagtaagt gctgcagttt gtccaaaaga cactactgca acaggtgtca tgatattttg
    22141 ccaaccattc acagaaaatt gcatcaatcc cattggtacg attgcccaat ggagaccaaa
    22201 catcactaga atttgccaga agaagcctac gattgctcca taaataactg gcgaaagtgc
    22261 taaaagtgtt tcaaatccag ccattaaaat atcagttgcg aatgtgaatg ctggtccaac
    22321 gagtaaaaaa cctaagggca ttgtaataag aacggtgaag aatggaacga agaaattcgc
    22381 gatcatctca ggtactaact ttttaattgg tttctgaatt tggctagcta acaacacaat
    22441 aaagataatg ggaatgacag aagaagcgta gttacgcgca atccagggaa tgccgaatac
    22501 tgtttgataa ataggtgctt caaaaatagt gccagaaaat atggtagtca gtggtgcttc
    22561 tgctgcacca ctcaaagctt ccagcgacaa gctaggattc attaatgctg ccccaagcaa
    22621 catccctacg aattcattta ctttaaattt acgggcagct gttaaaccga taataacagg
    22681 cataaattgg aatacagaat caccgatatt atcaaacata acaaatgtgc cagaaccgcg
    22741 gtcaaatgcg cctaagaaga caagtaatgc tgtcagtcca cgaatcatcc caccagcagc
    22801 tagaatagca aggaatggct ggaaacagcc cgatattcca tcaataagca tatcaaacgg
    22861 tctcattttt tctgttgaaa cagcctcatc aaaagtaagg tttcctgctt ttataacctc
    22921 ttcaaacacg actggcacat gattcccgat gacaacttgg tactgtcctc ccgctcgcat
    22981 taccgtcaca acaccgtcca tatttttcaa gatctcatca tttgcttttt cttcgttttt
    23041 taatttgaaa cgaagccttg tgatacaatg tgtcaaacca ttgatatttt ccttcccgcc
    23101 gacattttct acaattttgc tcgctaattc ttgatatttt cccatttcta ttccctccaa
    23161 tttattagtg gaaggtttag ccaactgaat gtcactaccc ttatttcatg cgttcttgtt
    23221 aatttatcct ccttttttta tttatttaca acgattctca tgcactaaac gcgcaatatg
    23281 gatcgttaaa tacatttttt cgtccgtgga catctggtag ttgaatatct cggctaaaaa
    23341 gtctgctatt ttctcaacgc aaccaaacgt atccttatat tttttttgaa taacctggta
    23401 caattcctca gaaacttctc ccacttctga tttttctcca tctaggacgc gtgtcagaaa
    23461 aaaacgtagg tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa atgtaatctt
    23521 atagtaatag cggacgatat ttagaatatc ctgtattgtt gtagtaaaca ggtaggtatt
    23581 gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa atccggcttc
    23641 atcttctgat aacttgatgt ggtatttatc cgagattttc ttcactgttt ttttcccgat
    23701 ggcatactct ttaggaaata gttgtttgat atcatacagc aatacatttg gtacaaatat
    23761 tcccattttg tgacgttcga tagccgtata caaatgatcc gtcaatgcaa tatacaccga
    23821 atcattcaac tggacttgta gagcctcttt tgtatattca atgatttcac aagaaatttc
    23881 taagtactca agaggaatcg cttgtgccaa ttcttggaac tgttgtgaaa gtccgtcatt
    23941 tgtcaactga aattttttgt cgatgcatga gtcaaaaatc gggtcaccca ctttcttttt
    24001 gaatgccaaa ccttttccca tcacgatcat ttcctgattt ctttcatccc tggttattac
    24061 gacgttgtta ttcagtactt tctcaatcag catgttttac tcctttccat gatatatagg
    24121 gctgtatcca aaacaaaaag aaaaacccaa aaagccgtcc atggaaccat gggagactca
    24181 tcaggtttag cttgcatcag gcaatcacta tccaagttgt atcgttttct ttatattatc
    24241 gaaagcaagc acttttgtca acgctttctt agcggctatt gggcacttct gactagctgt
    24301 cgtgattttt tttaaatctg gtaattacga cactaacgct cacagatgaa gaatctggga
    24361 agctctgttt tattttcact ctagcgctta ggtcaaaatg ctactatgct actgtcccaa
    24421 cttctaatat acggtgttcc agtaccagct tatcggtgat aagccaaatt tatgcaaaaa
    24481 tctggaaagc cttttttaca atttttttct tatcacctaa agctgaacgg agccctgtca
    24541 caaccacttt ttacacacca ctgctttgca gtttgtacat atctgcatac aaaccatttt
    24601 gcgctagaag tgtttcatga ttcccacgtt ctactatatg acctttgtct aaaactaaaa
    24661 tcaattctgc atcccgaatc gtggacagcc gatgggcaat cgcaatcgtt gtccgtcctt
    24721 gtcgcatttt tgtcaatcct tcttggatca atccttctgt ttcagtatcg atatttgctg
    24781 tcgcttcatc caatactagt attttaggat ctgtcacgat tgttcgcgca aaagtgatca
    24841 gctgtcgttg tccgctagaa tagctggcac cgtgttcgat cacttttgag tgatatcctt
    24901 ttggtaattc atcgatgaac ttgtttgctt gaacaaactc agctgcatgg cgaatctgtt
    24961 cgtctgtgat agttggattc atcatccgga tatttcctgc gatatctcca taaaacaaga
    25021 aggcatcttg caagaccaat cccattttct tacgtaactc ttccattgga tattctcgaa
    25081 tgtcatgatc atcaatcaag atctggcctt cataaaattc gtaaaaccgc atcaatacgt
    25141 tgatgatcga gctttttccg cttccggtgt gccccactaa tgcaacggtc tctcccggat
    25201 tagcgacaaa agagatattg ttcaatacat tgtgctctcc atcataagaa aagcttacat
    25261 tgcgaaactc gatcttacct gaagtaatcg cttgatctgc tccaggattt tgttgcggtg
    25321 catattcttc tgtatccatg attttcaaaa tacgactacc ggcaaccatt ccatctgtaa
    25381 aggtgctcaa aaagtccatc atttgagaca ttggattaaa aaatccctgg acataggtcg
    25441 taaatgcata gatcagtcca acttctacgg gagaatgcag cgcatcgatc ccaaacaaag
    25501 tcaacgccaa cgcaatcgcc aatgcatata gaaaattgat gatcggactt aacagaatcg
    25561 aatttgtttt gatcatcgca tagcgcgtgt ctaaatactc gttgttcact tgttcaaatt
    25621 ctttagctaa acgagcttct tggcgaaact gctggataat acgcatgccg gaaatcgatt
    25681 cattcaattt cgtgttcagc tgacttaatt tttcccgcat attccggtaa atacgagaac
    25741 tgaatttctg gtaataccag atgacgattc ctaaaatcgg caagaacacc aaagtccaca
    25801 aggcaatttc tccattgatt tgaaacatgg caatgaagga agaaacgact gcaaagatcc
    25861 cagtcaatac catcaaaaag acataccaaa attcaaatag cgtttctgtg tcatttgtca
    25921 ctcgagaaac agtcgatcct gccggtgtct gatcaaaata acgcatacct agtgtatgca
    25981 gcttttcaaa taatcttacc cgaacatact gataggtctt caatgaagcc atggaataca
    26041 aataccattg gaaaaaccaa atgatacttt tgactatgac tccaaacaag tacataccag
    26101 caaaaaacaa aataacttgt gtcgtagccg actgtttagc cagatgatca tccataaaag
    26161 tttggatgat ccttggcaat aggacattga tcacagacag ggcaaacgca aataagatag
    26221 caacgatgaa cgttcttcta aaaggtttgg cgaacttcat cagtcgtttg atgatcttct
    26281 tctgttcttt aagtggaatc gattttgtcc attccgattg ataattttca tccatcaggc
    26341 ctcgcctcct tctatcttcg cttctaattt ctgtttctcc cacattcgtt cataccagcc
    26401 gcctaatgca agcagctcat gatgggttcc tcgttcaatg attttccctt gatcgacaac
    26461 aatgatttct tttgcatgca tcacgctgct taaacggtga gctgcaatga tggttgtttt
    26521 ctcttttctt atttctttta aattatgcaa gatagcttct tctgttttgg catctactgc
    26581 agaaagtgca tcatctaaga tcagcaattc aggatttttg atcaatgctc gagcaatcga
    26641 aatcctttgt ttctgtccgc cagataaaga gactccacgt tctccaacta aggtatcata
    26701 accttttggt aattccttga tttcattgtt gataaatgct aaagaagccg catgctctac
    26761 ttcttcttct gaaagttttg gattagaaaa acggatatta tctcgaatgg tcatcgaaaa
    26821 caagaagtga tcttgaggca catagccgat tgatcctaat aaagcattca atgaatattc
    26881 tttgatcgaa catccgccaa attgaatttt tccttgataa tcatcgtatt cacgcatcag
    26941 taatttgatg attgtcgttt ttcccgcacc agttttccca acaataccta atgtttctcc
    27001 ttcttggatc gcaaatttga tttgttctaa tgctggtgat tcttctttcg gatagttaaa
    27061 agaggaaata gctacattca gctcgccttt tgccggagtc tgcactgcac ccagttgttc
    27121 cacgatatgc gtcttttcat gcaaaagttc attgacgcga tcataagaag catttcctct
    27181 ttccaatacg ttgaacaatc gaccaatcgc aaacatcggc caaacaagca taccgatata
    27241 gctaataaat gagaccatct gcccgattga gatcgtgcct tccaagacat actttccacc
    27301 taaaataatt gttgcaacat aagacagacc gataatcaag gtaatgaatg gatcaaataa
    27361 ggagtctaaa aaattgactc gtttgttttt aataattgca tcttcaatct tttcttcaaa
    27421 atccgcgaga tcttcttttt cttgtccaaa tgttttaatg actttgatcc cagtaatact
    27481 ttcttgtgtc ttatcattga tactagaaaa agcttcttgc gaatcacgaa aagcatcgtg
    27541 gagttttgac cccagcactc gtgaagtaac ggctagtaaa ggcagaggaa tcagcgcaat
    27601 cagtgttaag cgccaatcaa taaacaaaat catcgcaatg atcgttgttc cgcccgtgat
    27661 cactgaatct gcaaaggtca agataccagc tcctgctaca ttttggattg cattcaaatc
    27721 attcgttgca tgagccatca aatctccagt ccgatatttt tggtaaaaga gattgtccat
    27781 ttctgtaaaa tgtttgaata gctgctgccg aagttctttc tccaaccgag ctgcacttcc
    27841 ccaaatattc gtccgccaga tataacgaaa aagatattgt aagagagctg caaggatcaa
    27901 taataagatc caccaggaaa tgatctttat tgataaattt ttatcggcga tctcatcaat
    27961 gatgatccca atcacttttg gaggagctaa ctgtaccaag gccactgcga atagggagaa
    28021 aatcccaatt aaataatgtt tcttttcctg tcgaaaaaac caacctaatt ttctaaaaat
    28081 cgacatttta ctcctccttt gtctttatca agagcatagg attgtctgtg cttgtctttt
    28141 ttgctgaata gtctgaatag tctaacaaat tacgaaataa aaaacggact gaaaaaatca
    28201 gcccgcaaaa agtctttggg tcagaaccta ttttttccct tggttcttca tttgctgcat
    28261 catctggcga attttcttct cagatggttt ttgccccatt gacatcatca ttgatcgcaa
    28321 catgtcctca ttaacgggag gatttttttc taaatagtct ttcatatatt tacgcgctag
    28381 gaaaaagcca cctactgcgc cgattagcaa agcaataata gcaataagta cgacaatacc
    28441 tgttgatacc atataaatct ctcctttcta tttccgaatt gctctcaatg atttatttta
    28501 ctaaagttta ttgaaaaaag aaaggcgtat tctttagttt catgcaaaaa atagtggttt
    28561 ttttagagaa gaacaaaatt tctttcatta accagcaaga cacgccacga aaaaaaatta
    28621 ggcataccaa atctttttct ttaatttttt ctttctgagg tgtagaatgt aatcgtagat
    28681 aaataggaaa ggtggttttt tcttgaataa tcaatccaac aaacaatttc ataagcatcg
    28741 acttattgaa catacaaatg gtttatcttt agctgaagta aatggaacag tggatgtacc
    28801 caagggaaaa agttttttta aaacacttct tgcttactct ggaccaggtg cattagtagc
    28861 agttggttat atggatccag gtaactggtc cacttcgatc actggtggac aaaatttcca
    28921 atatttattg atgtcagtta ttttgatgtc tagtctgatt gctatgcttt tacaatatat
    28981 ggcagcaaaa ttaggaatcg tgacccaaat ggatttggcc caagcaatcc gagcacggac
    29041 aagtaagaca ttgggtgtca ttttatggat cttgactgaa ttagccatta tggcaacaga
    29101 catcgcagaa gtcattggtg cagcaatcgc cctctacctg ctttttgata ttccattggt
    29161 aattgccgta tttatcactg tgttagatgt tttcgtcctt ttattgctga caaaagtcgg
    29221 gtttcgtaaa atcgaagctt tggttgtctg cttgattttc gtcattttat tcgttttcgt
    29281 ctatcaagtt ggcttgtcta accctgattg gcacagtttg tttgccggat ttatccctaa
    29341 cagtcacaca ttctcagact caccggtcat cggcggcgaa actccgctga ctggagcttt
    29401 agggattatt ggtgcaacag tcatgccgca taacttatat cttcattcag cagtttctca
    29461 aactcggaag atcgatcacc aagacgaaga agatgtagca caagcggtac gcttttctac
    29521 ttgggattct aatatccagt tgtcaatggc ttttctagtc aatgcactgc tattgatcat
    29581 gggtgtggct gtattcaaaa ccggttctgt ccaagatcct tccttttttg gtctgtatga
    29641 agctttatct gatccatcag ctatgagcaa tggtatcttg attgctgtag caaaatctgg
    29701 tatcttgtct actttatttg ctgtagcact tcttgcttct ggccagaact ctactattac
    29761 tggtacgttg accggtcaag tcatcatgga aggcttcatc catatgcgta tcccaatttg
    29821 gctaagaaga ctagtcacac gattgctttc tgtgattcct gtattgattt gtgttttgtt
    29881 tacaagtaca aaaggaacga tcgaagaaca taccgctttg aataacttga tgaatgaatc
    29941 ccaagttttt ctggcttttg cattgccatt ctcgatgatt cctttactga tgatgaccaa
    30001 tagccaagcg gagatgggga gccgattcaa aaattcattt gttgtaaaac tacttggctg
    30061 gttctctgtg attagtctga cttacttgaa tttacgagga ttaccaggtc aaatcgaaag
    30121 tttcttcggc gagcaagcca cctcagctca actagcattg gctgatcaaa tcgcttatgt
    30181 aatcattgtt cttgtccttc tcctgttggc atggacgatt ggtgatttat ataaaggaaa
    30241 caaaaaatat gaacagcgtt tgcaggaaac gatagaagaa atcgagtaga aactgattct
    30301 tttacacaaa ggaggttgtg tcagaagtgg gcagcttcaa gaaataagaa ggaatttaag
    30361 aaaattgctt ttcaaatttt tgtgaaattt cggcttattt ccgaagaagt tacttctgga
    30421 acaccgtgta tcagaggttg tgaaaaagct gccttgcatc aagtaataag aacagccaag
    30481 taaaaacagc tttttatgtt tttaacttgg ttggacttat taccgcagat gcaagctttt
    30541 gagcaccgtt tattcggaaa aaggaactgc gacaagactt ttgtcacagt tccctttcct
    30601 acgattagtc aagtaaaagg tgtaagaaaa atagctcata aacgaaagcg acaaaatcaa
//
LOCUS       DAZUMT010000023        29240 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000023 DAZUMT010000000
VERSION     DAZUMT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.023"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..411
                     /locus_tag="ACG8FM_000942"
     CDS             112..411
                     /locus_tag="ACG8FM_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093623.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            411..581
                     /locus_tag="ACG8FM_000943"
     CDS             411..581
                     /locus_tag="ACG8FM_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093624.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(640..1119)
                     /locus_tag="ACG8FM_000944"
     CDS             complement(640..1119)
                     /locus_tag="ACG8FM_000944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093625.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            1164..1871
                     /locus_tag="ACG8FM_000945"
     CDS             1164..1871
                     /locus_tag="ACG8FM_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS5093626.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            1864..1992
                     /locus_tag="ACG8FM_000946"
     CDS             1864..1992
                     /locus_tag="ACG8FM_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093627.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            2050..2391
                     /locus_tag="ACG8FM_000947"
     CDS             2050..2391
                     /locus_tag="ACG8FM_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093628.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            2384..3130
                     /locus_tag="ACG8FM_000948"
     CDS             2384..3130
                     /locus_tag="ACG8FM_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5093629.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            3136..3822
                     /locus_tag="ACG8FM_000949"
     CDS             3136..3822
                     /locus_tag="ACG8FM_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5093630.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            3829..4662
                     /locus_tag="ACG8FM_000950"
     CDS             3829..4662
                     /locus_tag="ACG8FM_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS5093631.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            4679..5530
                     /locus_tag="ACG8FM_000951"
     CDS             4679..5530
                     /locus_tag="ACG8FM_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5093632.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            5527..5886
                     /locus_tag="ACG8FM_000952"
     CDS             5527..5886
                     /locus_tag="ACG8FM_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS5093633.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            5901..6062
                     /locus_tag="ACG8FM_000953"
     CDS             5901..6062
                     /locus_tag="ACG8FM_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5093634.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            6059..6364
                     /locus_tag="ACG8FM_000954"
     CDS             6059..6364
                     /locus_tag="ACG8FM_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093635.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            6364..6681
                     /locus_tag="ACG8FM_000955"
     CDS             6364..6681
                     /locus_tag="ACG8FM_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS5093636.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            6678..7064
                     /locus_tag="ACG8FM_000956"
     CDS             6678..7064
                     /locus_tag="ACG8FM_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5093637.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            7061..7396
                     /locus_tag="ACG8FM_000957"
     CDS             7061..7396
                     /locus_tag="ACG8FM_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093638.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            7396..7530
                     /locus_tag="ACG8FM_000958"
     CDS             7396..7530
                     /locus_tag="ACG8FM_000958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093639.1"
                     /translation="MKDWLEEASALVGCFINCAFISVIIALVGFVSLKVIILLWQLIF
                     "
     gene            7542..8111
                     /locus_tag="ACG8FM_000959"
     CDS             7542..8111
                     /locus_tag="ACG8FM_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS5093640.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            8108..8281
                     /locus_tag="ACG8FM_000960"
     CDS             8108..8281
                     /locus_tag="ACG8FM_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093641.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            8278..8583
                     /locus_tag="ACG8FM_000961"
     CDS             8278..8583
                     /locus_tag="ACG8FM_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093642.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            8607..8804
                     /locus_tag="ACG8FM_000962"
     CDS             8607..8804
                     /locus_tag="ACG8FM_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFS5093643.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            8801..9007
                     /locus_tag="ACG8FM_000963"
     CDS             8801..9007
                     /locus_tag="ACG8FM_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093644.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            9007..9303
                     /locus_tag="ACG8FM_000964"
     CDS             9007..9303
                     /locus_tag="ACG8FM_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093645.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            9379..9792
                     /locus_tag="ACG8FM_000965"
     CDS             9379..9792
                     /locus_tag="ACG8FM_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5093646.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            10476..10643
                     /locus_tag="ACG8FM_000966"
     CDS             10476..10643
                     /locus_tag="ACG8FM_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093647.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            10669..11013
                     /locus_tag="ACG8FM_000967"
     CDS             10669..11013
                     /locus_tag="ACG8FM_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5093648.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            11018..11299
                     /locus_tag="ACG8FM_000968"
     CDS             11018..11299
                     /locus_tag="ACG8FM_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093649.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVDIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            11402..11716
                     /locus_tag="ACG8FM_000969"
     CDS             11402..11716
                     /locus_tag="ACG8FM_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS5093650.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            11694..13388
                     /locus_tag="ACG8FM_000970"
     CDS             11694..13388
                     /locus_tag="ACG8FM_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5093651.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            13408..14586
                     /locus_tag="ACG8FM_000971"
     CDS             13408..14586
                     /locus_tag="ACG8FM_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5093652.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            14549..15235
                     /locus_tag="ACG8FM_000972"
     CDS             14549..15235
                     /locus_tag="ACG8FM_000972"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS5093653.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            15235..16395
                     /locus_tag="ACG8FM_000973"
     CDS             15235..16395
                     /locus_tag="ACG8FM_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5093654.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            16405..17280
                     /locus_tag="ACG8FM_000974"
     CDS             16405..17280
                     /locus_tag="ACG8FM_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093655.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            17277..17588
                     /locus_tag="ACG8FM_000975"
     CDS             17277..17588
                     /locus_tag="ACG8FM_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093656.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            17578..17931
                     /locus_tag="ACG8FM_000976"
     CDS             17578..17931
                     /locus_tag="ACG8FM_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5093657.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            17921..18322
                     /locus_tag="ACG8FM_000977"
     CDS             17921..18322
                     /locus_tag="ACG8FM_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093658.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            18315..18719
                     /locus_tag="ACG8FM_000978"
     CDS             18315..18719
                     /locus_tag="ACG8FM_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093659.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            18731..19336
                     /locus_tag="ACG8FM_000979"
     CDS             18731..19336
                     /locus_tag="ACG8FM_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5093660.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            19356..19718
                     /locus_tag="ACG8FM_000980"
                     /pseudo
     CDS             19356..19718
                     /locus_tag="ACG8FM_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19721..19903
                     /locus_tag="ACG8FM_000981"
     CDS             19721..19903
                     /locus_tag="ACG8FM_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093661.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            19921..23340
                     /locus_tag="ACG8FM_000982"
     CDS             19921..23340
                     /locus_tag="ACG8FM_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5093662.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            23391..24128
                     /locus_tag="ACG8FM_000983"
     CDS             23391..24128
                     /locus_tag="ACG8FM_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5093663.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            24138..26429
                     /locus_tag="ACG8FM_000984"
     CDS             24138..26429
                     /locus_tag="ACG8FM_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5093664.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            26453..28534
                     /locus_tag="ACG8FM_000985"
     CDS             26453..28534
                     /locus_tag="ACG8FM_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093665.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SDYTFAITYTI"
     gene            28551..28700
                     /locus_tag="ACG8FM_000986"
     CDS             28551..28700
                     /locus_tag="ACG8FM_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093666.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            28697..29143
                     /locus_tag="ACG8FM_000987"
     CDS             28697..29143
                     /locus_tag="ACG8FM_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093667.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            29145..>29240
                     /locus_tag="ACG8FM_000988"
     CDS             29145..>29240
                     /locus_tag="ACG8FM_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5093668.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT        10545 a         4365 c         6244 g         8086 t
ORIGIN      
        1 attgtagagg gaggtaaagt cgttggaaga taaaatcatt cttacaaaat cagagcttca
       61 agaaatgtta aacatcgagt atggaacgat aatttttagg agggataata agtgaaaaaa
      121 ccaacacttt cagagctgat agaagctgct gagaaggcag taaaaccaga cgactggtac
      181 cgacaaagtt taatcttgga gaagttccac ggcatgtcaa aaactacttt agttgaatac
      241 tgcaaggaaa tggaaacaat tcctgaattt gcagaaggaa tacttcgtcc aggtcactca
      301 accacattta ttcattacca tacttttatt tggtttttaa agtggaaaga cgcaaacaaa
      361 tatcgtgtaa aaagtttatc gccttcagat gttttgaagg aggcaagtta atgggcaaat
      421 tcaacagagc actagtattc agcgcaccgc taatcatcta cgctttaggc ctctggggaa
      481 gcagacaagc gttgataggt acgattgtgt acatggtttg gatttttata gggctggatg
      541 aagctgagta caaaacaaaa aagccagtcg ggagggactg actaaaaaag tctaatgtct
      601 ataagaaagg tttgacaata aatgaaagaa ttattttttt taataacttt catcattttt
      661 tctatcagtg ttttctacat taacctttgc gttgctagat ttgctttgaa aagttttagg
      721 aacagcaaac gaaaatagag ctataaaaga tacgaatgta gtagctacgt taaattgctc
      781 tttagaaaac gtcaatgtca ttggaatgat aattgatatc gatgagaaaa tctttgcgat
      841 aataactatc catttttcaa ttataaacat ttttaaatta agcaccatta gacaaaatgt
      901 agttcctata tagaggataa atgaagatag aaataaacat attagtccag ttttaaaaaa
      961 agagaagtaa tctatgtggg caaattctgt taatatgctt ttttgattta acacaatcaa
     1021 actactgttg gacaaaaatg acagtaagga aagagttgct ccgattacta aataaaaaat
     1081 agttgttaac aatattatat tatcaatttt tctgctcatg ttttcacccc accagaatta
     1141 tatcaaaaag gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg
     1201 tgactacgag cttacaagtt gcagaaagct tcgataaaaa gcacagacat gttttgacgg
     1261 ctattgatga actaaaagag ggggttgccg aaaattgggc agacctattt tgggaagata
     1321 cttatgttca tcctcaaaat aaacaatcat accgaattat ttatatgaat agagacggct
     1381 tctctctatt agcaatgggc ttcactggta agaaagcttt aagttttaaa cttcaatata
     1441 ttgaggcctt caacaagatg gaaaaagaat taaaagacca attgccatca attccagcaa
     1501 ctaaacgaga aatggcatta cttgctctag ctgcaaacga agaaacgaat gaaagagtag
     1561 acgctatcga gtctgattta aacgatttga agaacaatca acttctagct gaaccagatt
     1621 accgaacaat ttctaatatg gttcgcaaca aaattagagt tatttgtaat cagcagcatt
     1681 taaatagcaa agcaaaagca gaattgttta aagacttgaa tggcggaatt aagcgaatca
     1741 ctggagcagt agctagaaat cgtatcaagg caaaacagtt cgatgatgtt atcgatttta
     1801 ttaacaattg gatgccttct acagcgacga tgacaattat taagcaaatg gagttgttag
     1861 aggatgaata gagctgaagc gctaaaaata ggcgtaataa ttgctaatcg ctggtggaga
     1921 cacaataaac caaacatcct aagccaacaa catattgata agcaaaaagc atggcaacaa
     1981 ataaaaaagt gactcagccg accaaagcaa tgagtcacaa agaaaataca tctaaggaga
     2041 tgttaccaca tgaaaaaaga actttccact ctagatcaat atttgactga tcctagttgg
     2101 ggcaaatcga atatcaagga aacaagcaat cgaaaaatca gacgtaatct tttgacggat
     2161 gaagaactag catgtgatca agacgatttg ggaaattttg tgagtatttg ggatcatgtc
     2221 tatcttatcc atttatcgag gaagtccaaa aaaactgaat atatctatgt catcgaagat
     2281 ggcttgattg atgcgctaga agagtatgac agagataact tgattgatat ctcttattac
     2341 ggatcaggta agaaatacat tgctgaaatg gaggcagaat ttgatgagtg aaaaggaaca
     2401 acctttgaaa aatagaagtg ataatacgct ttttaatacc ttatacaaaa taaatgtgaa
     2461 agatgttact gaaaaacgaa ataacctcac ttatctttcg tgggcatggg cttgggcaga
     2521 agttagcaaa gtgtgtgagg cagtagatta tgaaatctat catgatccag aaacatatct
     2581 gccgtatgtc tttgataaga aaacaggcta tatggttttt accagtatca cagtcaacgg
     2641 agtaaagcgt gacatgtggt taccagtcat ggatggtgca aacaaggcaa tgaaagatga
     2701 gccatatacc tacgaagtca atgattatca gtggaataac gaaacgaaga aaaaagagat
     2761 tgttggaaaa atcgaaaagc gagttgaagc agcaactatg tttgatatca acaaaacgat
     2821 catgcgctgt cttgtaaaaa atctagcaat gtttgggcta gggctatata tatttgctgg
     2881 cgaagatatg ccagaagatg tctcgatgct tgaaccagct actcaaagaa gcaaaaagct
     2941 attcttggat gctttacaac tggttgctaa caagtacgaa aaatcaattg atgaagcaat
     3001 tgttgcattg actgatgcgg cttctataac cgctgatgac agtaaatgga ccaagagaga
     3061 cttgggcatt ctaaaacgag gcgttaactg gattgaagat cagtacagag aagaaacaaa
     3121 agagaagtga tatgagtgtt taaaccattg atcgattcat attcagcggt tctgaaaaag
     3181 ttcaaaggaa aagacatagg tgcaacgatc aatgaagaag tgaacattga tcgactaaag
     3241 acgatgtatg acggatatga tggcgatcgg attattgaag ttcgatttat tgatccacgt
     3301 cgatttactg tgcagcaacg aaacttcatc tatgcgctga taggcgatat tttcatcgat
     3361 acaggcatgc caacggactt ctggaaggaa ttcttttact tccgttttga aggcgtcaca
     3421 gggcgcaaaa taagcctcaa agacgaatcg aatacaactg tgagtgatgc taatgtctta
     3481 gcaaatatca tcttagattt catctttgaa catcatattc ctttcaaaga aggctatgag
     3541 attttaccag cgaatcaaga atattacttc tacaaatgca tcacaaaaag agtctgctgc
     3601 atctgtggca aaacaggagc tgacatcgat cactttgaca aagcgctagg aagacgaaag
     3661 cgcaaagaag ttgatcattc agagtacaca tttgcagcac tctgcaggat tcatcacacg
     3721 gagaaacaca atataggtgt gatcaatttc aaaaataaat atcaaatcaa agggatcaag
     3781 ttaaaccagg aaacaattaa gaaacttaga ataggagggt aaaaaatatt gtctgacaac
     3841 aaacgctact actatttaaa actaaaagag aattttttcg atagtgacga gatggttctc
     3901 ttagaaagta tgccagatgg ctatatttat tctaatattc ttctcaaact ttatttaaga
     3961 agtctaaaac acgaaggtaa gttgatgttt aatgacagga ttccatttaa ctctacaatg
     4021 ctcgcaacta ttacaagaca ttctgtagga gtcgtagaaa aagcggtaca aattttccgt
     4081 gatttgcagc ttattgacgt attagataac ggagcaattt atatgtctga tatacaaagt
     4141 ttcattggaa aatcctcaac tgaagcagat agaaaaagag aatacagaaa gaaaatagaa
     4201 gaggcaaaac ggaatttaat aactggagga caagtgtcgg acaaatgtcc agacaaaact
     4261 acaccagagt tagagataga gatagagaaa gatatagata tagataaaga agaaaagaaa
     4321 ggtaagtatt ctgacgaaca cttacgcctt gctaaaaaat tgcaaagtaa tttaactgaa
     4381 gattttccaa aagaaatgaa caaagtagat atcgaaaaat gggcagacac aatcaggttg
     4441 atggaagaaa gagataaagc gtctatagaa gcgattgagt atgtgatcaa ttggctacct
     4501 acaaatgaat tttggtttgg aaatattaga agtgctaaga aattgagaga aaaatttgag
     4561 aagctcaaat tcgaaatcaa agcagacaag aataatcata aaaagcaaag tcaaaaacta
     4621 cagtacagca atcctagtga atatgacgac ttgccaattt aaaaaggaga tgcatcacat
     4681 ggaaagccta gcaaatgcta tggagaaact aataagaaga gtattagtgc aaagcgggaa
     4741 atgtccagaa tgtagcgaac ctttgtatag ttggcgagct aaaaataagg atggttcaga
     4801 acgttgtaaa ccaacatgca tgagttgtgg ttataaagcg ttacgtgtga aagaggatat
     4861 acagaccgaa cggatatata acgacagctt aaaagcacga gcgttgagtt tttttcaaaa
     4921 tggttcggta ttaacagata aaactttgtt taaatgcaaa atggagaatt atcacgtagt
     4981 ggaccaagaa acgaaaattg ctttagaaag agctaaaagc tttgtaaatg atgtcttact
     5041 gaaccatcct gcacatttca ttctatcagg gaaatcagga agcggaaaaa gccacttgtc
     5101 aatggcgaca gcttgggaaa tacttgagcg ctcaaattat gacaagaaaa tactttttat
     5161 aagctatcaa gagttattag agcaaataaa gttttcttat aacaatgctg aactgagaaa
     5221 agaaattgaa ggatcgctta tagccgatat taaaacaact gatttggtgg tttttgacga
     5281 tattggagct gaattaggta gtggggtatc aaatagtagg cagtttacaa acaacacgtt
     5341 aaacacgctc ttagaagcaa gacagaacaa ggcaacgatc atcacaacaa acttatctgg
     5401 tcctgaacta agagaagcct acggtgaaag aattgtttct aggatattta agaattcaga
     5461 aggttatgcg ctgaaattcc agcaaacagc agacaagcgc ataaaaccag tgaaaggtag
     5521 tatcgcatga ataaataccg taataaaaaa actgttcatc gaggtatcaa gtttgattct
     5581 atcgcagaag cagagtatta cgatctagcc ttgtggcaag ctgaagcgaa tggctggaaa
     5641 gtaaaacttc aggaaagatt tgagctgatg ccgaaatttg aactagacgg aaagaagtat
     5701 cgcaagatcg agtatattcc cgacttcaca ttttataaaa acggcaaact tgtcaaagtc
     5761 gtagatgtta aaggaatgca gacaaaagac tttaagatca aggcaaagct gttttgtcat
     5821 caatatcaag tgccgttgat attagctaaa aaatatcgga atacgttcaa ggaagagcgt
     5881 ttttaaagag gtggtctatc atgacaacag aagaagtgat tcaaatgcga attcgaagca
     5941 ttcaacgtga gattgacgat ctggaacgaa caaaggcagt gatggtcaat gaaacggcga
     6001 gaaaggcaat cgatttgcac atagagaact taagaaggga aattcgtaga ttggaggaat
     6061 gagcgtggat aagaaagcag caatgaaacg aatcatcgaa ctgacacatt ctgagaattg
     6121 gcaagaagac aaagaaatag ttgcagaagt ccaaaagctc ggtaaatcaa tgtggactga
     6181 aaaaaccaga cggagaatgc cgagaaaaat tgcaatctgg cacggtgacc gaattctagt
     6241 aacaggtacc gctgaacagt tatctgaaat tactggtctg agcaaaaaca tcatctggga
     6301 tagagcgaag aatatggata ttgattctaa aggaagacag tttaggtatg tggaggagaa
     6361 ataatgaatc tcattacaca atacagtgat atcatcctca agaaaatcat gatgaagatt
     6421 cagaaagaca aaaaatcaaa agaacgagcg gaattagtta agttggaaat ggctgaaaca
     6481 ggggcaggag tgcgaagtag caggcattgg aaggcagcag caaacattga attttattac
     6541 aacgaaattc aaaaagggtt cgatcaaatg cgtgagctgg atcggcaaac aaattggagc
     6601 aagaaacttc atcaagatcg tttcaaattt gtagagaaat ataaagagat attagacgaa
     6661 tacatggagg gcagcaaatg ataccgagat ttcgatcgtg gtacacacca tttaaaggta
     6721 aaacaattgg acaagaaatg aaatatgggc aagcaggaag gttgatcact catgctgaaa
     6781 tggctccaga taaatatgtg cttatgcaat ccacaggact gaaagataag aatggcgtag
     6841 aaatatttga aggggatgta gttctggtta atgtaagtaa tggttttgat catttagtta
     6901 atgaaaaaac ggttgtacaa gaatccgagt ttcattccgg attagtttgt aaatcattaa
     6961 ttagtggaat ggaatataga atattcagcc accaaaagat gggatacgaa tatgaagtca
     7021 ccggaaatat acacgagaat agcgagttat tggaggtaga gtgattatct tagctactga
     7081 ctacatcgat tctttaaaag atgaaaatgg gaatgtccca gcattgctaa attgtggaat
     7141 taaaagtaag aatagaatca ttttgttacg gatatcggct gatttagcaa aatatcttta
     7201 tctgagcaag acagcagtat attacactgc ggctacacgt tatcagtaca agggaaaaac
     7261 aatcagtcag gattattatg atcgtttaat cagtcctgac atacatggaa aatcaaaatc
     7321 agcgatcaag ttatttggcg caatagagat atatgccgat gattttccaa atttatggtt
     7381 gaaggaggaa cagtaatgaa agactggtta gaagaagcaa gcgccttagt tggctgtttc
     7441 ataaactgtg cgtttatatc tgtaattatt gcactggtgg gatttgtatc attgaaagtg
     7501 attatattgc tttggcagtt aattttttag gaggaacagc gatgaataaa caggaagcaa
     7561 tcaaaaagtt agaaagtatt aaagtgatag gaaatgatgc actagctgct tgctataacg
     7621 agagtataaa tgcaggtatt acgttaatga aaaaaataga cgaaccgaag aagctagtta
     7681 ttccacaact cgtggccggt tggcttgaga aatctacgga cccttttacg aaagctgaaa
     7741 aaatagcgta tttaatcaaa tctaaagatg gtgattcata ttatttctgt gattggtttg
     7801 tacgagatgg catagtgacg caagaacaag gagaagaatt actcgcttgg gcaacgagac
     7861 aatcatatga aacactattg agcttgtaca acggctacga ggtcgagaaa gagccgttgt
     7921 acagtgtaat aattgctggc gagtaccttg tcaaggagat cagttgcagt aatgaagtta
     7981 aactagtatc aacagcttac ctgccacaat accttgcaca ccactaccaa ctgaccgaaa
     8041 aacagatcaa agcaattgat gaaagatact ggccatttgc tgtgccagtg gaagaggtgg
     8101 cggaaggatg agtagacaga taatgctcca tataccagac ggaacaaagg caatgacgat
     8161 taccattgtt gctgcacaaa aagacggtgg gttggcgttg aaaacaaaaa gcattgatac
     8221 caaacagatt ttagaaggca gagatgtcga gatagagatc gatgaggagg aagcggaatg
     8281 aatgttcaag tttatttaaa aagagatttg gccaggtttc ctcgaagctg ggatacaaat
     8341 tcccacccat ccgaggcaga agaagagggc tatctaatgt ctgctaacat gatagaaatt
     8401 acaagagatg atgcggatga gtttgataaa aaaagatcat ggtactcggt tccgaatcca
     8461 atgtatacgg ctgtaataga agattatcat atatccgaca gatttattct gattgatcta
     8521 gaaaggccta agaaacgaat taaacagtat agacgttgtg gattttcaac aaagaaatgg
     8581 tgataacaaa acagaggtga agaaaaatga acaacagaca ccgcagagta gcaaaactaa
     8641 gaaatcaaga actgaatgta gcgaaagtaa agttcgaacg ggagtatggt gtttctgcag
     8701 aagaaacata taaagtggca agtcagtgtg ttgctgatgc aagtgatgct attcgtaagt
     8761 ttggaatttc gatactagat attaaatggg aggatacaga atgaagattt atgtagtaaa
     8821 gtttggaaat caattttaca gaagcgatga acgttctata ggggctaaca cattatccat
     8881 tgtagacata ctccaaagcg caagatggtt tgataatctc gaagaagcta accaagtttc
     8941 acgacgactt ggtggattaa cgcaagtata tgaactggtc actgtcgatc atgagggggt
     9001 agagtaatga aactaaaaga cggattttac gctagtagtc atggtatcgg cggtttgatg
     9061 ctagatatgc cgacaaagaa ccctaaaaca cgtgagaaac caaaattcaa agtcggtgac
     9121 atggttcgct gtgaagcaga aggatttatc tatccatttc gtggatatgt agaaaaggta
     9181 ttgtcgaact cagcaatcat tcgtattgaa aacacgatgg aatgtgacaa gtggttagcg
     9241 aaaagcaaag agaatttagc tgtagcgaga ttagttgaca tggaagtaat cgagagcaaa
     9301 taaaaaagcc ggatcgctcc gactaacata ataaaacaga caagtttatt atatcacata
     9361 aaggagcggt ttgacttgat gcaattgtta cgagaggtag atttcaaaca gacaagatgt
     9421 aatgcgagag atgtgctgaa gaactttcgg cgtttggagc ggatggcagg tcgctctttg
     9481 atagatatta agtctccaat tattacggat atgccgaagg cgccgaagca cggcaataag
     9541 gcagaggacg cgatcattca gatgatggat atagaagcag agagagatgc gattttagcg
     9601 gctttgatgg ctcttagtct gattagccgt cagatactct actacagctt ttgtgtgcca
     9661 gatagcttct caaactacag aattagccgt gaagtgggtt attcagaaag aagtatacaa
     9721 cggatgaagt cggaagctct aatagagttt gcagaagcat ataaacacgg aagaataatt
     9781 gcttataaat aatttggcgg ttttttggcg gaatgatggc ggtttttagc tatttaccag
     9841 tgatattatg gtagtgtcga aagattagtg ataggtctga gacaaaataa taataaaagg
     9901 aacatcgttt tattattgtt tcacaattaa gcttcgatag acagcaacgg aaatattaag
     9961 aataaggatg tgaatttcaa ctccttctaa attgttctta ttatctatca tccgttgctg
    10021 tctattgttt ttttaattat tcacacgata actaaaggtg agtgaagaga aatgattcca
    10081 ttaataattt caatttttgc gctctgtctt aatgtctata tgattggatt taaaaatggg
    10141 caaaataaaa aatagtagca gccaagaata attttatagt gtcactgtgg cggaaagggt
    10201 agacgcttaa aaataaggtc aatacgtcga gggatagcct taacgtttta tgatttgacc
    10261 atgcaatgtt cgattcattg ccagtgactt tggcaaccga gggttggaaa tgggcgctca
    10321 aagtacacga gcaaggcgag gtcgatagta atcgatggaa tcggtgtagg ttgcttgata
    10381 gagctataac tgcatctcgt ttatgaggtg tagtttttac agattagatc actctttgag
    10441 tggtcttttt attttacaca aaggaggtaa caagaatgta tagaccacaa tacttagaac
    10501 agaagtatga agtaatcact gtgcaaaacg gtaacggtga gatagtacga aagtatagaa
    10561 gaccaataaa gagcgataca tacaaacgaa aggaaagcaa tgaagttatt ccattgtatg
    10621 gcaaaagaat agctaagcat taaataagat tgcgaaagga gacggaatat gaccgaggaa
    10681 ttctatagat ggctattaca gttgataaga gaagatcgtt tagttaagtt ctatcagtct
    10741 cctaaatggc gcaggcttag agagaaagcg atgaaacgag atcactatga atgccaagag
    10801 tgtagaagac taggtaagta tcatagagta gaaaacgttc atcatataaa ggaagtcaag
    10861 gatagacctg acttagcttt agatttagat aatcttattt gtttatgtgt tgaacatcat
    10921 aatgaagttc atggcagata tcttacagca ttagataaac aagagaagaa gatagaaagc
    10981 tttgctaact tcgatgcaag tgaaaggtgg taagtgcatg atcatcaatg ataatggcag
    11041 agagtatgat acagaaaaga ttgaagagta ttcatcttat actcagggat taattaaacg
    11101 tttgatatac gttcgctatg tagatattag ggatctgtta tcagataact gttgtagtaa
    11161 atacaaagtg aatcaagtaa gagaagcgtt gaataaagat aataacgtcg aaagaataaa
    11221 aaatgttttt ggatatagta ttgaagagat taattattac attgacttcg ctgaagcttt
    11281 cattccgatg gtgagataac ccccccttaa aataaatcgc aaattttttg ggggtgatga
    11341 aacggagggg gctgtcagga aaagagattt tttcgaactt tatcatgaaa ggagggctaa
    11401 aatgtttaaa aacgaattgt ctcaaaatcg ctacagagaa aaattacgcc gctctttaat
    11461 aagccaattg gaaagtcaga aaacaaatat tgagccattc ttagataatg ttgatcgtta
    11521 tatcagttta tgggaaacgg cgatatcact ggaagaagat atatccgaga acggcattag
    11581 actggagaat ggtaaaaaga atgaatcagt agcgttgctt gtttctgtca acaaacaaat
    11641 gggattgatg ttggataaac ttgccattac tcctgaattg gtaggtgaag caaatgaatc
    11701 aattcctgag ttataagcat attgaaaatt ggttcaaagc tatagaagaa ggcactatca
    11761 aggtatgcaa agagcaatta ttgctaaaaa attatctaga agaaagagtc tttactagag
    11821 aagatattta cttcgataag cagatggtag aggattcaat caatatacca gcacaatact
    11881 ttccattcga attaattccg tgggaaaaat ttctacaatg ttttatttat ggtgttcgat
    11941 ggaaaaaaga taaaacacta gtgttcaata gatatctttc attaatggga cgtggtaatg
    12001 gtaaaactgg ttttgcttct tggaacaact tctttctact aaccgctaaa cacggtatta
    12061 aaaattatga tattgatatc tatgccaata atgaaagcca agcaaagact agttttgatg
    12121 atgtatttaa agtaattaaa gatcatcctg atttagataa aaaagtattt aaagctacga
    12181 aggaagttat tcaaaatatc gctacaaaca gcaaacttcg ttataacacg gcaaatgcta
    12241 gaacaaaaga tgggaagcga ccaggtgcaa accgctttga tgaaattcac gaaaatgaag
    12301 attattcaat gataaatgtg gctacttctg gtggtggtaa aattcgagat tatagagaat
    12361 tttatgatac aactaatggt catgttcgtg gtggtccgct tgatgacatt atagaagaat
    12421 caaaaatgat tctttctgga gaacttggaa ttgacaagga tggagcagaa ttttctagtt
    12481 tgtttccatt tatttgtcgc ttggataacg ataatgaagt tgatgatccc gacatgtggg
    12541 aaaaagcttg tccaactatt aattacaatg cagatttaaa acggaaaatg tttcaagaat
    12601 actctcaaat gcaacgtaat gctggtttaa gacttacgtt catgaccaaa cgaatgaaca
    12661 gacctatgga agatacacga tttgctgttg cttcatatga tgatgttctg catacgaaag
    12721 aaaaagaatt tcctgaaaaa atggatgaag tgataggaac agtcgatttt gctgatagac
    12781 gagattttgc cagcgttggg ttgctaggaa aatacgataa agatgtttat tttacacaac
    12841 atacttttat ccacgaatca gcccttcgat tacaaaacat caaacgagag gttatagata
    12901 tttctataga tcaaggaaaa tcacagatcg ttcatggaaa aaatatagaa gctgattata
    12961 ttgtaggttg gtttcttgaa atgagtaata aatattatat taaaaaaatc gctatggata
    13021 tgtaccgtgc aaaaatattg aagcccgctt tagaagaagc aggttttact gtggaaattg
    13081 ttcgaagcgg atctgttaca catggtatgt taaaagatct ggttgatgac ctttttatta
    13141 atcaacgttt attttttggt gacgatgcga ttatgcgttg gtattgcatg aatgtatatg
    13201 aagagcatat ttctaatgga aatatacgct atgaaaaaat agaacctgaa actagaaaaa
    13261 cggatggctt tttttcattc cttcatggtt tgaatttttt agatgatatt tatgattctg
    13321 ctcctgtaac agtcacaaat agctcagtag aaaatacagg aactggattt actcctctag
    13381 tattctaact tgaaaggagg tgagaaagtg gggatttttc aaaaggcggt aggatacttc
    13441 acaaaaaaag caacggttac tttagaagaa tacttttgta aattgcaagt tgattttgtg
    13501 tatcgaaaat ttgcgattga aacttgtatt gatttgattg caaatgcgat gagtaaagcg
    13561 gaattcaagt catatgaaga tggaaaaaat aaaaagaatg atctttacta taggctgaat
    13621 gtagctccta ataagaaaaa taatgcaaca gaatttagaa aaaaactgat caggagatta
    13681 atattctaca atgaagtatt gatcgtttct ccgtctaata attctagcga aatatttatt
    13741 gcggatagtt gggatgtcac agaatatgca ttgaaagatg atgtgttttc tcaagtgcaa
    13801 attaacaaca tagtccttga tagagaattt ctagaaagtg atgttatcta tataaaatac
    13861 gcagatcaac aaattaggca actagtcgat gcgtattatc aagcgtatgg gaaactcatt
    13921 tctagtgcca tgaatgttta caagcgctct aacgctcgta gatacgtact gaaagggaat
    13981 ttattccgac cgcaagacaa tacaacacaa gatcaaatca ataaaatgat gacatcacaa
    14041 tttaaggctt ttatggaagc tgataatgca ggtgcggtat ttcaattaca aaatgagtac
    14101 acattagaag atttcagcgg aaacttccaa agcaattcaa gagatataaa aaacttaata
    14161 gacgacatct ttgagatgac agcagcagcg tttcacgttc cgaaaaacct actaaaggga
    14221 gacatgagtg ggttatcgga tcaagtggac gcttttttaa tgttcgaaat cataccgatt
    14281 gctgaactta ttcaggatgc gtttaacgct agtctctatg aagtagaaga atacttgtca
    14341 gggaattttg tacgtgtgga tacaactatg atcaagatta ctagcttcaa agatttggtt
    14401 gacgctattg atgtaggcat tagaaatgga gtatttacaa tcaacgaagg aagagaacgc
    14461 gttggaaatg atcgctctga taaggcgatg gcagatgaaa tatttataac taaaaacaat
    14521 caacaagtat cgaaaggagg tgaggcgaat gacgacaatg aaaacatttc tagcagtaaa
    14581 gaatgaaggc gcagtaccgc aaatttttat tcagggattt attggttcta gttggttctt
    14641 tgaagggaat actgacaagg gaatcaaaaa tattttggat agtctaggtg atcaagaaga
    14701 aattgaagta gtaattaatt caaacggtgg agacgtattt caagggattg ctattgggaa
    14761 cttacttaag tcaaataaag caaaagttaa cgttgtgatt aacggcttag ccgctagtgc
    14821 tgcttcaatt atcgcaatgg ctggcgatac tataaaaatc tacaacaatg cacaattgat
    14881 gattcaccgc gcttccacat acggagaagg aaatgtcgat gacttccgta cgattgctga
    14941 ccaactggaa tcaattgata aatcggtaaa ggcttcatat aaaacacgat tcaatggcac
    15001 agatgaagca ttgcaagaac ttcttgaaaa agaatcgttt atggatgcag aaacagcttt
    15061 gagttatgga ttggtcgatg aaattatcga tgcagaaaat agctcaggta ctgaagctaa
    15121 aaaagaacaa agcgttgaag aaattttgaa tgacgttaaa gaaaaaagag cagaaaaaat
    15181 tgctgcattt acagcagcat taaataaaac atttggacaa ggagatgtaa aataatgaca
    15241 gttaaaaatt taaaaggtgt aacagctgca agcgaccaat tgatgaaagc ttttaaagat
    15301 ggtaacgaag aatcttttag cgcagctatg gtaagcttat ctaaggaaat tcaggataaa
    15361 attttagaag aagcaacagc aaaaaatcaa gatcaattag tattaatgaa ccgtggtcag
    15421 cgtgtattaa ctacacaaga aacaaaattc tataacgaag tagtgaaaaa cgaaggtttt
    15481 gcaggggtcg aagaattagt gccagctact gtatttgaac gtgtatttga agatttagaa
    15541 caatctcatc cactattgca aaaaattact tttgttaaca caactggtgt aacagaatgg
    15601 attgtgtcac gtggagtcaa tccagcatgg tggggtaaac tttgcgaagc tgttaaaaaa
    15661 gttttagata atggctttga tgtaattaac atgaagcagt tcaagctatc aggttatatt
    15721 cctgtatgta aggcaatgct tgatttaggt ccagtatggt tagatcgtta tgtccgtact
    15781 gttttagtag aatcattgag aattgcatta gaacaagcaa ttgttgatgg tactggtaaa
    15841 gatatgccag tcggaatgat gcgtgacatg agcaaacaaa ctagcggaga atatgctgaa
    15901 aaaaaagcag aacctattac agctttagat gctgtaacta tgggcggttt gatggcgcga
    15961 ctatcaaaat tcaatatcga aggtgtgaat gatccgattt atcgtaatgt gaatccttct
    16021 gatgtggtcc taattgtgaa tccaactgat tactggtcta aagtattccc agctaagact
    16081 gtactaactg ctaatggaga atacgtacaa gtattgccag taccagtttc agatttgcag
    16141 tcaacggctg tgccagaagg aaaagcagtt attggggtag cctcagatta cttcatgggt
    16201 gtaggatcta cactaaaaat tgaagcttca gatgaatacc attttgttga agacgaacgc
    16261 atttatctag ctaaacaata tgcaaacggt caacctaaac gtaacgatag tttcattgta
    16321 ttagatatta gcgctttggg aactactact acaactacaa aaccaacaac cacaacaact
    16381 acaacacaag cgtaggtgat cagaatgaag tatattcttt gtcagccggc aatcaatcgg
    16441 tttaaatggg agcttgaagt ttgtttaact aatctgaaga aactaggaat caaagatatc
    16501 gtattgcttt tcagcagaca cgatgatcag attcctattt tttttgagaa ggaatatggt
    16561 gttgaagttc atgtgtacga tgatctgcgg gacgacaaag agtatattcc ttcgattaaa
    16621 ccatatttat ggtggaaata tttagaagaa gatcattcgc gtgaggacga ccgatatttc
    16681 tatatcgatt cggatgtcat tttcaataaa agaattaatt tgcgcaaatt gccttctaaa
    16741 gatgatgttt ggtattgtag cgactgctgt agttatctaa gtcttgatta tattagaagc
    16801 tgtgaaaacg gagaaaatat tctaaaagat atggcaaaca ttgtaaatgt tacagtagaa
    16861 tctttggaaa ctataaacac taattcagga ggcgcacagt gggttattaa ccgccctaaa
    16921 gcgaattatt ggaaaaaggt ttatctggat tctaatcggc tatatcgcta ccttagaggg
    16981 caaaaaacaa atataaaaat ctggacagcc gagatgtggg cacagctttg gaacatgatg
    17041 tatttcaata ttggtcctaa agttcacgag gaattagact tttgttttgc tactgatcca
    17101 atagaaaaag ttaaagaagt aaaaatctta cacaatgctg gagtaacaac aaacgatgaa
    17161 gatttatttt tcaaagggag atacgtgact tctacgcctt ttgatgaaga tttatcattt
    17221 gtaaacaaga aaaaatgctc ttacgcatat gttaaagcaa ttaaggcggt ggttagatga
    17281 cgcctgaaca agtgactgaa gaattgctaa tagctgtgaa ggataatatt tacgttacct
    17341 ggaacgaaga agatgagtca attaaaaaga tgatagctaa aaatgctgtt tatcttcaaa
    17401 gtaaagtgag tacaacactt tctttttctc ctgaaagctt agaatacgga ttgctaatcg
    17461 aaagatgtag atacgactgg aatcgtgctt tagatgagtt tgaacaaaat ttcgctagtg
    17521 agttattagg tttcattcaa cattatgcgc tacaagaata tattgcaggt gatgtgaatg
    17581 gcgaataatc gtagactcga agaaacattc aacgatggtt ggttaaagat tttgacgcaa
    17641 actaccaaaa gaaatgaact aggaaaaaag attggtgtag aagatacaga aatcacttct
    17701 ttaaaattta gaaatctttc catgagagat agtgatataa cagctatgga tgcgatggga
    17761 tcgaaattaa ctaagaaagt aaagactcca tttcatccaa tcgccaagaa atttaataaa
    17821 gatcaatatt ttatcgtaat cgatagtatg cgttacaacg ttatctatgc cgattacgat
    17881 aatttttata tctattttta tcttgaaagt gtgggtgaat atggtgattg ataattctaa
    17941 agaaaaagaa cgtttaaata agcaaatttc tgctatcaaa acttccttag aagagcattt
    18001 tggcctcaaa ctctttcaag actccgtagg cgaggatgag ctacctgatg attttaatta
    18061 cttcattctc gaaacaggag aaatagaaat gatcactgag ccaaaatata gcgtgggtca
    18121 aaatctatat ctaactttct attcagaaaa tagagaagat ttaacaggag attcactaga
    18181 tattatttca ttgattcaaa atcgttcgat tcgttttcag agaatggatc ccaatcattt
    18241 aaaactagag aaccaagatc gctatatcga tcaattggta tttacgttta gacgattatt
    18301 gaagagtgat tgtcatggct aaaaatagtt gggagctaaa aataaatgga catgatgaac
    18361 ttcttgtgcg gatggaacgc tattcaagcg agagcgaacg actgattaac gaagcattga
    18421 aatcaaaagg ttcggctatt gcagtggata ggattacaga aaaaattcct gtttctgaag
    18481 cagatttaag aagagggcac caacacgcaa aaaatagtcg tccacttaag actcaataca
    18541 ttaatttggg tttcatcatt agacctacaa gaaaatttga gtatttaaaa tatcctgatt
    18601 tggggatagg tacttctaaa agaaatcagc cggacgaatt tatgagaaga ggattaggtc
    18661 ttgcacttga tccaattaca gaacttctga ttcgtcaatt cgataaatta aataaatagg
    18721 aggaacaaca atggctaaaa caacaacagt aacaactttt gacaatataa gtattaaaag
    18781 aatttctttt aattttaaga atgcaacaaa tgcaatttca actgattgta atggacaatt
    18841 agatggcgaa acagaaatgc agagaattgt aaaaaaatgc ggttcaacag aagtaaaatc
    18901 gaaatctaaa ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta
    18961 tcgacgcttc aatgggttga aacaagatga acgtattaaa tcgggaattt actcttatgg
    19021 tcctgattcc gtaggggaag atttctctct tgctgcagag atcgtggatg atttcgaaga
    19081 aaaaaataag ctgattggta tgttagcatg cacttcgaat acaggattaa cattctctat
    19141 tgaaaatggt gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga
    19201 atttggtaaa ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca
    19261 atggatgacg aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc
    19321 cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg
    19381 aactaaacga tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga
    19441 tgatcaatag agattttaac acagacgagt ttgcaaaaat gtcgatggga aaaggctcta
    19501 tggatattac agttattcaa ggtgcaaaag ccgtatatgt agcttatcgt caagcgaaca
    19561 tggtcgatta catttcattc gatgaattta tagataaatg agattttgat atggaggttg
    19621 cagtagctgt atacagtact atgatgttca aacaagcacg tgatgcctat caaaaagaat
    19681 tcgaaaaagc aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga
    19741 aacgtgggtc gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt
    19801 tttaagcgat atctgtttag atgacatttt gcaaatggct tacaacaaga gcgcttatga
    19861 aggatggaag aactatgcaa taaatcaatc ccagaaaaac taaaggaaag gaggtaaaaa
    19921 atggctaaaa agagaacgga agcagaagta actttcatag ctaacgatga cggattgaaa
    19981 tctacgttaa aagaaatcag tgctgaatta actaaaaata gagcagaatt aaaactagaa
    20041 caagctcaat tgcaacagac tggttctgaa tcagacaagt taggaagtaa attatcttct
    20101 ttagaaaagc agtatgaatt acaaagtcaa aaagttgaag taactagcca acgtttagcc
    20161 aatgcgaaaa aatattatgg agaaaattcc accgaagttc agaaacttga aagagaactg
    20221 attaatcaac aaacagcgca acaacgtttg tcaaacgaaa ttgataaaac gagtaatgca
    20281 ctagctcaag caaaaggcga aatacagacg tacgagtcta caatgcaaca gttggatagc
    20341 gaacaaaaaa atgttcaagc tagtgcttct ctgattgaat ccgaatacaa aaaatggcaa
    20401 gcaactgctg gtcaatcagc ttctgaagcc gagaaattag cgaaagccca agaatatgtt
    20461 tctcaacaat ctgaaaatgc ggagaaaacg atagatatcc taagacgaca gttagaagct
    20521 acacagtctg aatttggcgc tacatccaca gaagcaatgc agatggaagc gaagcttaat
    20581 gatgctgaac gtgaatttga agagttagga caagctgcta aaaatgtaga tacaactaac
    20641 ttggacgata tcggaagcaa aatagatatg aataatttaa tggaagcttc tgacgtttta
    20701 agcgacattg gcgataagct tacagaatta gggaaacaag cagtggactc tgctaacagt
    20761 gtaggtagtt cccagagtaa gatacaagct aattttggtt tgtctaaaca agaggctgaa
    20821 gaattaacga atgtagccag agacatttat tataaaggtt ttggagaatc gttagatcag
    20881 tccacagatg cattgatttt ggtaaagcgt aatttaggag atttaaataa tcaagattta
    20941 caaaatatca cggaacaagc tatggtccta gaaaacacca tgggcgctga tatggatgaa
    21001 acgttacgtg gtgtaaatgg cttaatggtc aatttcggct tgagtgctca agatgcaatg
    21061 gatttaatgg tttcgggtac tcaaaacggt ttagataaaa cgcacgaatt aggcgacaat
    21121 atggcagaat atagccaatt atggagtcaa atgggatatt cagctgatga aacgttcgga
    21181 atgcttcaaa atggtttaga tgcgggtgct tataaccttg ataaagtcaa tgacttagtt
    21241 aaggaaatgg gaatatcgtt aacagatggt cgatttgagc aaaacatgga tatgtttagt
    21301 gaaagtacta gaaaagcttt tgaagagtgg aaaaatggcg gaggaacaca aaaagacgtt
    21361 attaattcca tgattcaaga ttttagcaat atggatggtc aatacgacca attaaataaa
    21421 gcttcgacaa tttggtctgc gcttggcgaa gataatgcga tgaaagttgt ccaatcttta
    21481 actgatgtta accatacatt tgatgatgtt agtggatctg cacaaaaaat gaatgaagat
    21541 tctactactc cgttacaaga attaaacgga aaaatagctg aattaaagga ttcattagct
    21601 cctataggca acacaatcat agatgcactc gaaccagtaa ttgattttct aggaaagatg
    21661 gctgatgcgt ttaataatct tccacaacca gtacaggatt atgccgtagc gattggcgga
    21721 ttgactgctg catttacttt attaatgcca ataatagttg gcttcatggc tctaggtggt
    21781 cctactacat taataatagg agcagttatt actgctattg ctggagttat agcaattata
    21841 aaaaactggg gcgcaattac tgactggttt aagggaatat ggagtaaatt cactgattgg
    21901 ttgggtggta cttgggaaag tataaaagaa ggtgcctcat cagtttggga tggagttaaa
    21961 gaaacctggt ctggatttgt agattgggtt caagatattt ggcaaggagt ttctgattgg
    22021 tttggagagc tatggagcgg attagttgaa ggagcttcca acatctggca aggagtccaa
    22081 gaaacttggc aaacattcgt tgattgggtt tcaaatattt ggaacggagt caaagaagta
    22141 tggtcgatta tttgggcaga cattgtagga attgttcaaa taccatggac attaataacg
    22201 tcattgattc aagctggtat taatattatc gtgggtattt ttgatgtagc tggacagtta
    22261 ttaggcgcag cttggcaagc tgtttggaca cctatttctg atttccttaa aaatacttgg
    22321 gatactatga cacaatggat aagcatcgct tggaacggaa ttgtaactac attccatact
    22381 atatttgatc cagtagtggc atggtggaat ggtatatgga catctattag tactacggct
    22441 tcaaatattt ggaattcaat tagtgcaaca gcttctagta tttggaacag tatcaagaat
    22501 acaatcacta gcttggtaca agcagctgct acagtaattc aaaatatttg gtcaactgta
    22561 tctagttggt taggtggaat ttggaattca atcagctcta cagcatcaaa tatctggaat
    22621 agtgtgacta gtagtataag caatgctata aacgcagcta aaagtgccat tcaaagtgtt
    22681 tggaatagta tatcttcgtg gatcagcgga atttggaacg gtatcaaaaa cactgctttg
    22741 aatctttgga atggaattac aagcactatt agctctaaag taaacgatgg aaaaaatgca
    22801 atttcaagcg gttggtccaa tctaacaggt attgtttccg acatattcaa taatgttaaa
    22861 agtacaattg ctaacatttg ggaaggtatc aaaaagactg ttagcgctcc gattgattgg
    22921 attagagata aaatcagtgg catctttgat aatttgaata tttcgatacc acatattccg
    22981 ttaccacatt ttaaattgag tggggaattc aatccattga agggaaaaat cccaacgttg
    23041 ggtgttgatt ggtatgcgaa aggtagtgtg tttaattctc cgaatattat cggtgtcggt
    23101 gaagcaggac ctgaagcagt tttacctttg aaaagatctg ttttgcaaga aattggtgat
    23161 cgtatcttga gtagcacatc agtttcatct agggcacaaa cgattcaacc tgtgaataac
    23221 tacgaattca atttcacaat tgatggtaac gcagatgagg ttactatgaa gcaaacaact
    23281 caacaaatca ttgatagcat tacaaaaatt caaaatgata atgcttcggc atggcgttaa
    23341 acaggagagt atttctcctg tttttttagt attaaaaagg atgtgaaaaa atgactgatt
    23401 gtatacattc tataatcgat ggatttcctg attatttgca taaattggct ttagctgaaa
    23461 gaccaaccat accttctcca aaaagacaga gagttgaaac ttctgtttta ggtaggttag
    23521 gtggcttagt acaagattac tcgtttgaag atatgtcgtt tacattgcac tataactatt
    23581 tagaggatgt ggaagaccat caagcgttca agcaatcgtt ttatatcatg cgtcattggt
    23641 taaactatgc aaagaaatta gaattctctg acgatcccaa cgtctattac gttatccaga
    23701 ctatcgatat tggggatgca gaaaacgata ttgttgaatg gggagagttc gatgtaaata
    23761 ttactgcgaa accattcgca agagttcaag aagatgtacc tataaccgta gataaaccac
    23821 agtcatttaa cttgctgaat aatagtttag aagaaagttt tccaaagatt atcatcactc
    23881 cttcagctac ttcatgccag ttcatcttaa atgattatgt gtttagtttc gaaggcttag
    23941 tagcaggaac tgatgtagtc attgatagtg atttgatgct ttgctacgaa gagcaatcgg
    24001 acggagatat tttagatcgg tccaacaaaa tgaagaccat gcaatatcca acgttgcaag
    24061 tggatattaa tcattttaat tgtactggtt tgagcaaaat acaaatttat cgtaacgggt
    24121 taaggtaggt gaaatagatg atcgataatt taataactat ttacgataaa aatgacgcga
    24181 ataatttagc tgaacattta tatgatacgc aaggtttagg cgctttgtca gactggttaa
    24241 cagctactgt tagcaataaa ctaaacggag ccgagatatt tcagggtact tattcaataa
    24301 acggaactaa tgcagatttg attgtagaag gacgtattat tcagtgttat gtagatgaaa
    24361 atcgagcaaa acagcgtcta cggatttatt atgcaaaaac ttctgtaata ggcaatacga
    24421 tagaagtaaa agctgaacct attttcaatg atataagaaa atcggtgttg aataaatatg
    24481 acagcggaac agaaaagatc actgctactc aggcatggca aaacgcaaaa gttttagcga
    24541 aaccagctat cccttcgcag ttttctttct cgtcattagt agatacgctt gctaatgtga
    24601 agatagaaaa ggcgaatttt ttagaattct ttggtggaaa agagggatct attctagatc
    24661 gatttcatgg tgagtttcta aaagataata acacattacg tcatgaaaaa agtctaggta
    24721 cggatcataa aatcaaagcg atttatacta aaaacttaac tggtcttgac ttagagattg
    24781 atgctcaaag tgttttagtt ggagtttatc cattcattag cagctcttca gaaggagaag
    24841 acgagatcac tctaccagaa gaagttattt tcacggatta cgtggatgat tatcctgctg
    24901 gatatgtttc ttttgttgat tttaaagaca aagcgactga tgtagccaca ttaagggaag
    24961 ctgctaaaga ctggttgaaa acaaacatag ataaacaaaa accacaagtg agtggttcga
    25021 ttgaattagt accattgagg catcaaagag gctatgaaaa atttgttgat ctagaaaaag
    25081 tttcgatggg tgacggagta gatgtgtatc atccacagtt aaaagtgaat atgtcagcga
    25141 gaattgtgga atatacgttt aatgttctaa ccaattcata cgataaatta gttgtaggaa
    25201 acgtcaaaac aaacttctta gaaaacacag agaataatgt cagcaatttg attaatgatg
    25261 ccattgatca attgaaaaat ggtggcgaaa tcagtgattt aatcaatgat attgtagatc
    25321 atcaaactga tatgattact ggtcaaaatg gtggttacgt tttattagat cctaaagaag
    25381 cccctagtcg tattttgatt atggacacac cagataagaa tactgcacgg aacgttttgc
    25441 aaatcaacaa cgctggtatt ggtttctcta aaactggcat taatggaacg tatgacaccg
    25501 catggacgtt agatggcgga ttcaatgcct cgtttattac agctggtgag atagtaggga
    25561 ttactattag aggtactaca ttaattagtg atggcactga ttatagaaca agtatagcta
    25621 atggcaaaat gacttggtac tcaaaaaaag ttaacaaaga tattatggag ctagaagcac
    25681 gtgattatgt aagtgctgat gcaggtattg tatcatacac catgaaaact ggtggtggtt
    25741 tcatgattag aaatccacag gggaatttgg tttttagtac gtgggataat ggtaataaca
    25801 aaccgttttt atcttttggt gcgcccaatt tcaggtatag caatgctagt tatataactg
    25861 atggcgatgg tagttcttta agcattaatg gtagtgcggg cagttcatgg gagtttaaag
    25921 tagctggtag gactatgaaa tttactagtg atggtatgct aacgttgcca ggttgttttt
    25981 ttggttcatg ggaagatgga aaacttgcta ggtttgaaca atcaacggta caagtatata
    26041 aaaattttac tgttagaggt actaaaaact caaccgtacc aacagaacat tatggacaac
    26101 gactattaaa cgcttatgaa actccagaat attatttcgc tgattatggg gaagccgtta
    26161 caggtgatga tggtaaagtt cgtgttgata ttgatcctat gtttgccgag acagtaaatc
    26221 taagtcgata tatgacacat gtgacaccta cagagctagt tttgtgtgct gttactcatg
    26281 aagatattga ccatttcatc attgaaacta gtaagccaaa cgtattggtt agatggaatt
    26341 tagtggcaca tcgactaggg tatgaagatg ttagattaaa agaggataca gcatatgata
    26401 gcacagtgct tgaccaaaaa cgtttttaaa acgaagacaa ggaggtatat aaatggctag
    26461 cagtttatat aatttggctt tagatttcag caaagaatta aactacacca aagctattat
    26521 ggctcgtcaa ggtgacaaag gaattacggt gactgttaaa ccgtttttaa atggcttgca
    26581 gatggatacg agtggcggaa cctttacatt aaaaggaaca acaccatcta accgttacgt
    26641 agatagtgtc gctactagta taactagcga agaagtcaca ttttctcttg atggaacatt
    26701 tatgagtgaa gcaggatact ataaacactg ctacgtagaa tatagaaaag ataatcaaat
    26761 tttaacgacg caagatatca tttttttctc actaggagtg tctgacattt cgcaaggcca
    26821 agccgatgaa tatgtttcgc aattagaaga attgattcga aagtataacg aaacttttga
    26881 tgcttttctg gctgaaatta aaggtagaat ggatagctta aataaacaga ttactgattt
    26941 aactggtcaa gctaaaacac tacaagacaa gttagatgct ctgaaagaag aaatttctaa
    27001 attaggtaac ttacatgtga tgtactccaa cagcatcgac ttcgggggct atgattatag
    27061 tgggaatccg aatttaatgt ccaaactaaa atcgagcgat tttaacgttg gttaccacgg
    27121 gtcactaact aatgttaacg gaatgttaca ttttatgtct gatggtacag gggacattga
    27181 tatgtttacg cgtattaata cacctcagct tgctagtggg aaaacctata ctctgagtgc
    27241 gaaagttcga tttgatgaag gaactacagg agctattgat aaattacgtt tggtgtatcg
    27301 tacatcacca ggagaaaaga tattattgga agcaaatagt acaaatatta caacagatga
    27361 tgtagggaaa gaaataacaa tcaaaggtac agctaacgtt aattatcaaa tcacaaattt
    27421 agatcgattt tatattagtg ttagtttttc taacagggat aaaataaatg gtggatttaa
    27481 attgtacgac atcaaaatcg aagaaggctc aacagccact ccgtatcagc caaacttact
    27541 agatgaccct tactgggtag gtaaaacatc gttgggtaag aatattgcaa ataaaggtac
    27601 gacgttccca attaaatcta gcgcgtacga aatatataaa ggtaacacgg aagaagagct
    27661 tataataggt caaacgtata ctatcacgct taaaggaaca aaacccgcaa gtcaaacctt
    27721 tgtagcatat aattattgga acgttaattt tggagatcta aaaccagttg agggattgac
    27781 agacgtatgg tctctaacat tcacaccaac gaaacttgaa ccgggtttgc ctaaagacct
    27841 tcgcattttt cagtcgccta aagaaacggc cggcgcatgc cgaattgact ggctcaagat
    27901 cgaaaaaggc gacacacgaa ccccgaatat tgagcaatat aaataccgag gaatcggcat
    27961 gcgagactca aacaatccaa aagattatgt atgggatcta gaacctagct atgttgagaa
    28021 taacctagct acagatatca aggttgctga aatcacaggt aaagcaaaca attataccga
    28081 tgggaaagta tcggagatta attcgcagtt gactgcttca attaatgaag tagacaccac
    28141 agctaaggat gctcaaacaa aagcgaatgc taatgcgact gctatagatg aattagacaa
    28201 taagatcgat gaacgcatta atgatacagc tactaccaca ttaacagtta caaacgggaa
    28261 taccggatca gcaaagcttt atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt
    28321 aaacggaaaa cgcagtggtg gaaatgattc aacgatacta acaattccag aaggctatcg
    28381 gccaccaatt agttttgagc aactggttgg ctcgatagac cgttctactt tgaacagtgc
    28441 tcagttatct attggtgcag atggagccat taaatggcga agaaactcaa gttatggatc
    28501 ggattatacc tttgcaatta cttacacgat ttagaaaagc gtgaatcgat atgaaggcag
    28561 catatagacc aattgaacct tacggattcg agcaaatcat tgtgaatgat gaagaacatt
    28621 taccggaaga atgcacagaa gtcgaaccac cgattccaaa ttggaaaccg aaattcaatt
    28681 actgggaggg aaataaatga aaaacatttg gaaatatgga cgtactggcg gagagtacgc
    28741 aggaaaagta ttggacgaca tgcttgtatc cgttccttac acagatcagc caccgcttga
    28801 aggaattcgt gctgatggcg aaccgttaac aatcgctgat caaatgtttg atcctaaatt
    28861 gaatcaatgg attattttag cgaacgcact agatcacaac gatttaaaca atctcaaagc
    28921 aatgtatgag tcgttagaaa atgagaacgg cgatttaaaa cagatcaatg ccaaactcat
    28981 gctaagcgat gtagcaatta aacaggaaaa tactgcattg aaagaaaaag cggatagttt
    29041 agcacaaatc aattcaaaaa tgatgcttgc ttcgttacaa aatagcaaag acatttcaga
    29101 aattaaagag caactaaatc cagcttcaaa gggaggtgag tagtatgttt agttttagcg
    29161 atgtgaaaat gatgtatgat tggggctgtt tcactaacga acaagtaatg gttttcgttc
    29221 cgttgtgcat tactgaagaa
//
LOCUS       DAZUMT010000024        28010 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000024 DAZUMT010000000
VERSION     DAZUMT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.024"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..196)
                     /locus_tag="ACG8FM_000989"
     CDS             complement(<1..196)
                     /locus_tag="ACG8FM_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5093669.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTN"
     gene            complement(198..395)
                     /locus_tag="ACG8FM_000990"
     CDS             complement(198..395)
                     /locus_tag="ACG8FM_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5093670.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(789..2516)
                     /locus_tag="ACG8FM_000991"
     CDS             complement(789..2516)
                     /locus_tag="ACG8FM_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS5093671.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2509..3702)
                     /locus_tag="ACG8FM_000992"
     CDS             complement(2509..3702)
                     /locus_tag="ACG8FM_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5093672.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3889..4533)
                     /locus_tag="ACG8FM_000993"
     CDS             complement(3889..4533)
                     /locus_tag="ACG8FM_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5093673.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4627..4761)
                     /locus_tag="ACG8FM_000994"
     CDS             complement(4627..4761)
                     /locus_tag="ACG8FM_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS5093674.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4763..6895)
                     /gene="feoB"
                     /locus_tag="ACG8FM_000995"
     CDS             complement(4763..6895)
                     /gene="feoB"
                     /locus_tag="ACG8FM_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS5093675.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6892..7365)
                     /locus_tag="ACG8FM_000996"
     CDS             complement(6892..7365)
                     /locus_tag="ACG8FM_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS5093676.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7766..7990
                     /gene="nrdH"
                     /locus_tag="ACG8FM_000997"
     CDS             7766..7990
                     /gene="nrdH"
                     /locus_tag="ACG8FM_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS5093677.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8149..10308
                     /gene="nrdE"
                     /locus_tag="ACG8FM_000998"
     CDS             8149..10308
                     /gene="nrdE"
                     /locus_tag="ACG8FM_000998"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS5093678.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10358..11323
                     /gene="nrdF"
                     /locus_tag="ACG8FM_000999"
     CDS             10358..11323
                     /gene="nrdF"
                     /locus_tag="ACG8FM_000999"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS5093679.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11412..12551)
                     /locus_tag="ACG8FM_001000"
     CDS             complement(11412..12551)
                     /locus_tag="ACG8FM_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5093680.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12860..13573)
                     /locus_tag="ACG8FM_001001"
     CDS             complement(12860..13573)
                     /locus_tag="ACG8FM_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS5093681.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13827..14528)
                     /gene="nagB"
                     /locus_tag="ACG8FM_001002"
     CDS             complement(13827..14528)
                     /gene="nagB"
                     /locus_tag="ACG8FM_001002"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS5093682.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14762..16294
                     /locus_tag="ACG8FM_001003"
     CDS             14762..16294
                     /locus_tag="ACG8FM_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5093683.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16485..17687)
                     /locus_tag="ACG8FM_001004"
     CDS             complement(16485..17687)
                     /locus_tag="ACG8FM_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5093684.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17769..18458)
                     /locus_tag="ACG8FM_001005"
     CDS             complement(17769..18458)
                     /locus_tag="ACG8FM_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5093685.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18564..18932)
                     /gene="rplL"
                     /locus_tag="ACG8FM_001006"
     CDS             complement(18564..18932)
                     /gene="rplL"
                     /locus_tag="ACG8FM_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS5093686.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18985..19488)
                     /gene="rplJ"
                     /locus_tag="ACG8FM_001007"
     CDS             complement(18985..19488)
                     /gene="rplJ"
                     /locus_tag="ACG8FM_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS5093687.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19692..19819)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19898..20587)
                     /gene="rplA"
                     /locus_tag="ACG8FM_001008"
     CDS             complement(19898..20587)
                     /gene="rplA"
                     /locus_tag="ACG8FM_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS5093688.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20691..21113)
                     /gene="rplK"
                     /locus_tag="ACG8FM_001009"
     CDS             complement(20691..21113)
                     /gene="rplK"
                     /locus_tag="ACG8FM_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS5093689.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21456..22124
                     /gene="sdaAB"
                     /locus_tag="ACG8FM_001010"
     CDS             21456..22124
                     /gene="sdaAB"
                     /locus_tag="ACG8FM_001010"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS5093690.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22137..23009
                     /gene="sdaAA"
                     /locus_tag="ACG8FM_001011"
     CDS             22137..23009
                     /gene="sdaAA"
                     /locus_tag="ACG8FM_001011"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS5093691.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23100..24200)
                     /locus_tag="ACG8FM_001012"
                     /pseudo
     CDS             complement(23100..24200)
                     /locus_tag="ACG8FM_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24292..25179)
                     /locus_tag="ACG8FM_001013"
     CDS             complement(24292..25179)
                     /locus_tag="ACG8FM_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5093692.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25256..25801)
                     /gene="nusG"
                     /locus_tag="ACG8FM_001014"
     CDS             complement(25256..25801)
                     /gene="nusG"
                     /locus_tag="ACG8FM_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS5093693.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25902..26072)
                     /gene="secE"
                     /locus_tag="ACG8FM_001015"
     CDS             complement(25902..26072)
                     /gene="secE"
                     /locus_tag="ACG8FM_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS5093694.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26096..26248)
                     /gene="rpmG"
                     /locus_tag="ACG8FM_001016"
     CDS             complement(26096..26248)
                     /gene="rpmG"
                     /locus_tag="ACG8FM_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5093695.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26410..27054
                     /gene="cbpA"
                     /locus_tag="ACG8FM_001017"
     CDS             26410..27054
                     /gene="cbpA"
                     /locus_tag="ACG8FM_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS5093696.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27110..27613)
                     /locus_tag="ACG8FM_001018"
     CDS             complement(27110..27613)
                     /locus_tag="ACG8FM_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5093697.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27626..27672)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         8481 a         5788 c         4960 g         8781 t
ORIGIN      
        1 tattagtata agcagtaaaa tatagataat tcaccaatct atttattaaa ggaccgggat
       61 ctaattcata tttttgtttt tctaatttta aatatacctt ttgtaaaacc tcaagaatat
      121 cagaatattt aggatcttta cttatagata tatcattata taattcatga acaatttgct
      181 tagcattttt tttcatttta gcacttccct ggaattgctc cacctaaaaa accacctata
      241 gacattcctg caatacaagt agttgccttg gcccaatcga ccgtacattt atttttagtg
      301 caatacactc catttccata atattttcca ctatgagtgg tacccccata gataagttgt
      361 gtctctttaa tagacaaaat ttttaaatgt ttcataatct aatcacccct aaattaatgt
      421 ttttagacta ttattctaac attcattaaa tttcaaaaaa caattccttc ttgaaacgta
      481 gcaaaataat tcctgaataa gaatttaatc acttttaagt atataaacta tgtatattct
      541 ttaatatttt ttgagaatgc acttcgtaca taataaactt taatcacatt agtctttcat
      601 acctacgatc gatctctttg actcgcaccc ataaattcat aatagtttgt cggcgtcttt
      661 ggacgtttcg aagatgagct acgcattctc tttttttata gacaaaaaag atagagacga
      721 acatggagct tccaaatttg gatatttgat ccaattttgg aagctccatt tatttcgtgt
      781 ctgtcttttt attttttcca gctttcgact aattcttggt tctctttgat ccagtctttt
      841 gctgcttgct gtggatcttt tccattgttg atttccaaca taactttttc catatctttt
      901 tctgaccaat ggaaattgtc taatactttg taagcttctg gctggtcttc tttcaatcct
      961 tttcgaacca tcgtatggat ctgctcttcg cttcccattc cgttttcagg atcttctaag
     1021 tatttcagat catatttggc aaacatccaa tgaggtgtcc atcctgtaac tacaattggt
     1081 tcgtgttttt tgatcgcttg acctagtgca acggtcattg ctcctgaaga agatgtctct
     1141 actttccagt ctttcaagtt gtcatatttt tggatcgtgt tttcagcagc cgtaactaca
     1201 ccagctcctg gctcgattcc tatgattttc ttacctgcct gatcggttaa ctcatcgatc
     1261 gaatttacat ccatgtaggc tggcactgct aaaccaactt ttgctcctgt caaattggca
     1321 cctaggtctt cgacttgatc tttatattgt gcatattgtg aaccatgagt tttaggtagc
     1381 caagctgaaa ccatggcatc tgactcgcca tttgaaacag atttccacat gattgaatta
     1441 tctaatggcg tcatcgttac atcatagccc atttgtttca atacttcacc gactacattt
     1501 gttgaagcaa cttctgtatc ccattcaaca taagataagt tgatacgttt tgtttctttg
     1561 gcagatgaaa aactccctat accaatcgca cctaagatca ctactactac agcaccaatg
     1621 atcagtcctt gacgtttttt acttttctgt gttgttgctc ctgcagcttt tttattgttt
     1681 ggctggttca aatgctgtgt aaaacggtca atgatgattg ctaagataac taatgcaacc
     1741 ccgttgacaa atccattccc gacttgtgct ctttgcaatg ctgataatac gccgcgtcct
     1801 aagccgggtg caccgatcat agaggcaata acgaccattg aaagtgacag cattgttgtt
     1861 tgattgatcc ctgccatgat cgtactttta gctaaaggca attctagttt gaatagtttt
     1921 tgacgacttg tgctcccgaa tgaatcagaa gcttctacta attctttagg aacttgtcga
     1981 atccctaaat tcgtaaaacg aacggttggt ggcagagcaa agatgactga ggcaaatacc
     2041 cctggaacca tcccgatacc gaaaaatgca actgctggaa tcaaataaac gaatcctggc
     2101 attgtctgca taaagtcaag gatcggtttg ataatacttt gggctttact gctttttgcc
     2161 atcaagatcc ctaatggtac cccaatgatg atcgagatca cactagataa aagaacaagt
     2221 gtgacagtat tcatcaaatc attccataga ccttggttat aaataaacca tagaccgatt
     2281 aatgtgaaca acgtaagtcc tggacgtttc ttagaaataa agaaagctgc taccgtcaat
     2341 aacaagataa acaaaggagc tggaatcact aacagtaaat ttgtaatccc cgacatcact
     2401 gcctgtccaa tcgtttgtaa aaatgaaaat aggccagaaa aagttgtcgt aaaccattct
     2461 gtgatatttt ctacccagtt tgccactggt aattgatagt tcaatagatt attcatgttc
     2521 actcacctcg cttgtttctg ctaatgcttc aatcacacta cctcggatga cgacaccttt
     2581 taattttcca tcctctacaa ctgcaagtgg agtcggtgag tcataaataa tattgaaaat
     2641 atcacttacc accatgtctt tcccaatcac cgtcacattt tcatctagga agtcgatcaa
     2701 tggacgtttt tgcttcctag cttctaacgc ttgatctgca gtgatgatcc ctttcaggtg
     2761 tctctttctg tcaacagcca tcagcatgct gacttcttca ttacgcatcc ttgtcaatgc
     2821 aacgtttggt ccatcagatt cgatattcgt cgtcaatgca ggtaccatga tgttttgagc
     2881 cgtaagcact ttggaacgat cgacttcttc cacaaattca cgtacataat catttgctgg
     2941 attggtcaat atttcttcac ctgttccgat ctgcatgatt tcgccgtctt tcatcaaggc
     3001 gattctgtcc ccaatccgta aagcttcatt caaatcatgg gtaatgaaaa taatcgtttt
     3061 ttgaacattg gcttgcaggt ccagtaattc gtcttgcatt tctcgccgga tcagcggatc
     3121 aagagccgag aacgcttcgt ccatcagcaa gatctcagga tcatttgcta aagctctggc
     3181 taggccaacc cgttgttgca tcccaccaga taactgacta ggatattggt ctttaaatga
     3241 aagcaaacta gaattttcta atgctttttc tgctttttct tgtcgttctt ctttaggtac
     3301 accacggact tctaacccgt attctgtatt ttccaagatc gttcgatgtg gaaacagacc
     3361 gaagttttgg aaaaccatat tgattttatg acgacggact tctcgcaatt cttctttgct
     3421 taacttcgat acatcttctc catcaatata gatactgccc gaagttggct caatcagacg
     3481 attcaataga cggatcaatg tggatttccc actcccggaa agccccatga tcacgaagat
     3541 ttctccttct tttacgtcaa aattcacgtc atacacacca accgttgcac cggttttctt
     3601 taaaatctcc gttttcggct ggtgatcatt catcatatct aaagcggctt gtgtcttttt
     3661 accaaatatc tttgttaaat gttttacttc tacttttacc aaacaaattc tcctttttta
     3721 tgtaaattca tttttctttc tgaaaaaagt gaccacacta tgttaacatc atgtagtcac
     3781 tttgtcaaaa atgaaagaaa tttgcttgtc caaactgaaa aaaacttggc taaatctttg
     3841 taaataaaag gtttagccaa gttaatattt ttttaatatt tcacacaatc attctgcaag
     3901 ctgatgctcc gggtaaaaaa agtttgtcat acgctgtagt gtatattcat ttccaataaa
     3961 atgaacgatg tcccctgccg taaatttagc ataggggcca ggtgaaacaa gcagttcttc
     4021 attatgagaa attgcgataa ctgtggcgcc agtcgcctgc cagatattca actcgctaat
     4081 tgaacggtcc agatgcattg ctttttctga taaagataat tcaaacggaa gtaaaggatt
     4141 catcttatgt attcgcttcg tttgattgac cagctgatcc aataattgag aaaaatgatt
     4201 cagctcatct tgttgcttct ccacacttgc catgatttct gcttttgtat cttggatgga
     4261 ttgtacatct tgatattgca ataggaaatc ttttgctttt tccttcgaag caacaaatgc
     4321 cccacttcca tgccgtactt ccatgattcc aagatctacc agcacattga tagccttgcg
     4381 ggtagtctca ggagaagcat tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc
     4441 acccacttta tatcggtttt caacgatacg ttcggctaaa tcaacagcaa tctgctggta
     4501 gcgcgggcgg acgatttttt tctttccaga cattttcttt cctctttctt tttttacttt
     4561 ccctcatctt actatgctct agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac
     4621 gactgatcat cgcttgatct gttccttttt tactgggcaa gctgtttgac agtcatcaca
     4681 attcttaccg gattttaaac gagtatagac aattgcgcct gcacttccaa agattaacac
     4741 acttaataag aatgtcgcca tcctattcct ctccttctaa tgacaatata gtataaaact
     4801 ggttcttgga ttttggctga cggaacaaaa cataaacgag tcctatcatc agtaaaatag
     4861 caagatacgt ccctaacgct acctgtccac cctccaataa gtgtcccaac tgataaatca
     4921 caaaactaac taaataagct aaaccgcatt gatagcctat ggcgatccaa gtccatttct
     4981 tggcattcat ttcccgatgg atagccccaa ttgccgcaaa acaaggcgca caaagtaaat
     5041 tgaaggctaa caatgaataa gctgctgcgg gagtatatgt gtgttgtagc acttgccaga
     5101 tttctctgcc attttcggat acttccgcat gaccaaacaa aataccaaat gtgccaataa
     5161 tattttcttt agcgaccaat cctgtaatcg ctgcgactgt tccttgccag tttccccaac
     5221 caagcggctg aaaaacaact gccaggattc gcccgaaaaa ggctaagata ctttggtctt
     5281 cagaaacagg ttgagcataa aaattataag aagagctgaa ccagatcacg atactagaaa
     5341 caaagataat cgttcctgct tttttaacaa aagacttgct gcggtcatag gtttgtctta
     5401 ggaccgctga ccattgagga aaatgatatg cgggcaattc catgataaaa ggagctgtat
     5461 ctccagcaaa caaacgtgtt tttttcaagc tgattccaga gaaaataatt gcagtcatac
     5521 caagaaaata ggcagaagga gctacccaag attgatgagg aaaaaaggcc cctgctacta
     5581 aggcaataat cggcaacttt gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac
     5641 gacgatcttg ctcgttttca atcgtgcgac tggccatgac gcctggcact ccacatcctg
     5701 tagcaatcag cataggaatg aaggattttc ctgaaagacc gaatttcctg aaaagtcgat
     5761 ccatgacaaa agcgatcctc gccatatatc cgcagtcttc cagaaagctc aagcataaaa
     5821 acaatataat caattgcggt agaaatccta acactgctcc cacgcctgcg atgatgccgt
     5881 tcaagatcag ctcctgcatc catcccgcta cttgccattc agctaacaat ctgccaacat
     5941 gttctggtaa ccatgtacca aacaatgtgt cattgatcca atctgtcccc attgttccta
     6001 ctgtttgaat agccagataa tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta
     6061 accaacgatt cgttgcaatc tgatcgattt tgtcactcat cgataattgg aaagaatcat
     6121 ttgacacaac acagagtgta catagttggg taataaattc ataacgttca ttgaccagaa
     6181 tcgtctcact atcgtcgtcc aatagtttct ctgttaactg gatcagttgc tcgatctctt
     6241 tttgctgaga aactgctatc tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt
     6301 tcattgcata ccagcgtttc ttgccgcttt gttctcctag tacatcttca atttcatgta
     6361 aagctgcttc taaacgtgga tcatatgtac agggaacagg aatagaaggc cgcttttttg
     6421 cagactcctg aatcttttcg attagttttt cgattccttg ctttttcaat acgctgattg
     6481 gtaaaactgg tacaccaaga ccataggaaa gcttttctag attgattttt tttccttctt
     6541 ttttgatgac atccatcata ttcagaccaa tgatcatagg aatgtctaaa tccaataact
     6601 gcgtagttaa ataaagattt cgttctaaat tggtagcatc aataatgttg atgatcagtt
     6661 caggctgttc ctgaaccaaa taatctcttg caatcacttc ttctggtgta taaggagaca
     6721 aagaatagat gccgggtagg tcctgaatat gaatgttcgg attttttttc aacgatcctt
     6781 cttttttttc tactgttacg cccggccaat tccctacata ttgattcgaa ccagtcaaga
     6841 gattgaacaa gctagttttc ccgctgtttg gatttccgac taacgcgata ttcattcttt
     6901 ttcctccctt tgtatcaaga ttagttcggc ctctgacttt cgtaacgtca gttcataccc
     6961 tcgcactgtc agttcgattg gatctcccag aggggctagt tttcggataa ctatttttac
     7021 atttcgcgtc aatcccatat ccattaaccg tcgcttcact gcgccttgtc cgtggattgc
     7081 tacgacgatc ccagtctctc ctgcttttaa ttgatcaaga gaagtccagc tttcagagga
     7141 agcagtcttt ttattgacaa agatttgacc tagtatttcc cgacttactg ccactcgatg
     7201 actttttacc agtaaaatgg cattgttgcc tgataaattg acgacgacga ttttacttcc
     7261 ggcaacgatc ccgatatttt gcaaatggcg ttttgttttt tcttctgttt cgattcgttc
     7321 gacttgataa acgaaattct gctttgcctg cgctaaggtc tgcatattga ttcctctctt
     7381 gtgaaataaa tcatgtatta aaatcattca ttatatgttc taattttctt acgtgttacg
     7441 caaataaatg agaatcattc tcacatagaa tactctcggt aattgagaat gtcaattata
     7501 cttgtgatat tatgagcgaa tctttagtat tgatagataa aaatgagaaa aaaatatttc
     7561 ttcatagttt tccacaattt tttctctatt aaatttgcta ttttcccaca cgatacttgt
     7621 tttcagctct tttttcaaaa acacaaaact tagtattcca cagtttccac aacacaatat
     7681 gtagtgtttg ttgtttgact tttttactaa gagttttata ctatctatta tagataataa
     7741 gaatgtgtta gaggaggcac tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat
     7801 gcaatgtaag atggtcaaac ggtttttaac agaaaatcaa attgcttttg aagaagtcaa
     7861 tatcgatgaa cagcctgaag cagtagactg gttgaaagca caaggattcc aaagtgttcc
     7921 tgtcattact tctgatgcag aaacagttat cggattccgt cctgatcagt tgcgtaaact
     7981 tgccagctaa catgactaaa agaatcaaaa cgtgatacat ggatttgaag ggcttgagac
     8041 agcaccagtt tttgtcgcaa acttccaaat ccttttttaa agcttaaaaa caatgcaatg
     8101 atttgcaatc aagcatcatc aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa
     8161 aatttacaag atgtcagtta tttcaagctg aataatgaaa tcaatcggcc agtcaatggt
     8221 caaatccctt tgaataagga taaagaagct ttagttgcct tttttgagga aaatgtcaaa
     8281 ccaaatacga tgacttttct atctattatg gaaaaaatca attacttgat cgatcaagaa
     8341 tatatagaag cagaattcat tcaattgtat tcaccatcat ttattgaaaa gctatatcaa
     8401 tttttggacg agcaacattt ttctttcaaa tcttttatgg ctgcttacaa attttattct
     8461 caatatgcgt tgaaaaccaa cgacggttcc gcttatctgg aaacttatga agatcgagta
     8521 gccttcaatg cgctttactt tgcgaacggg gatgaagaat tagcacttgc tcttgcagac
     8581 gaaatgatcc atcaacgata tcagccagct acaccttctt tcttgaatgc tggacgtaaa
     8641 cgtcggggcg aattggtgtc ttgtttcttg actcaagtca ctgacgatat gaactctatc
     8701 gggcgctcaa tcaactctgc tttgcaattg tcccgtattg gtggcggcgt agggattact
     8761 ttatcaaatc ttcgtgaagc cggcgctcct atcaaaggat atgagggtgc tgcaagtggt
     8821 gtcgttccag tcatgaaatt attcgaagat agtttcagct attctaatca attaggtcag
     8881 cgtcaaggag ctggcgtggt ttatttgaac gttttccatc cagacattat tgctttcctt
     8941 tcagcaaaaa aagaaaatgc agatgaaaag attcgcgtaa aaactctttc tcttggcgtg
     9001 atcgtacctg ataaatttta tgaattgaca cgtaaaaacg aagatatgta tttatttagt
     9061 ccatatagtg tcgaaaaaga atatggtgtc ccattctctt atgtaaatat tacagctgag
     9121 tatgatcatc tagttgcaaa tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt
     9181 gaaaatgaaa tctcaaaatt acaacaagaa tctggctatc cttacgtaat caatattgat
     9241 acagcgaacc gcgagaatcc aatcgatggc aagatcatca tgagtaattt atgttcagag
     9301 attttacaag ttcaaacgcc ttctgtgatt aacggcaaac aggaatatga agtattagga
     9361 acggatatct catgtaactt aggctcgaca aatatcgtga atttgatgca aagccctaac
     9421 tttggtcaat ctgtgcgtac gatgactcgt gcattgacat ttattacaga tgcatctgat
     9481 atcgatgtcg taccaccgat ccaaaacgga aataagctga atcatacgat tggattaggt
     9541 gccatgggct tacacactta tcttgcaaaa gaacaaatgg aatatggctc agaggattca
     9601 ttagacttta caaatattta cttcatgctg ttgaattatt ggacacttgt tgaaagcaac
     9661 aatatcgctc gtgaaagaaa acaagttttc cataattttg aaaaatcagc ttatgctgac
     9721 ggcacttatt tcgaaaaata cgtaactgga gaattccagc caaaatcaga aaaagtcaaa
     9781 gcattgtttg aaggcatttt tatcccaact gctgaagatt ggaatgcttt gaaacaagca
     9841 gtaatgaaag atggactgta tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt
     9901 tcatatatca acgatacgag tgcaagtatt catcctatca ctcgcatgat cgaagaacgc
     9961 caagagaaga aaatcggtaa aatttattac ccagcgcctt atctatccaa tgagacgatt
    10021 ccgtattaca cttctgctta tgatatggac atgcgtaaag taatcgatgt ttatgcaatg
    10081 gcacaacaac acgtcgatca aggcatgagt ttgactttat tcatgcgttc tgaaatccca
    10141 gaaggcattt atgaatggaa gacaacaacg aagcaaacga cacgtgattt gaatatttta
    10201 agacattacg ctttccacaa aggaatcaaa tccatttatt acgtacgtac ctttactgat
    10261 gatgcagaag aaatcggaag caatcaatgt gaaagctgcg tgatctaata tcatagatag
    10321 attcgcttat tcatatttta attaaaggag cattctcatg tcagaaacat attatgaagc
    10381 aataaactgg aatgaaattg aagatatcat tgataaatcc acttgggaga agctgacaga
    10441 gcaattttgg ctagatacac gtatcccact ttcaaatgac ctagatgatt ggcgtacatt
    10501 gtctcaattg gaaaaagata cagtaggaca tgtatttggc ggtttgacac tgctagatac
    10561 tgtccaatct gaaagtggca tggatcagtt gcgtaaagat gtccgcactc cccatgaaga
    10621 agccgttttg aacaatattc aattcatgga atccgttcat gccaaaagtt attcttctat
    10681 ttttagtaca ctgaatacga aaaaagaaat cgaagaaatt tttgactgga caaatacgaa
    10741 tccttatctg caaaaaaaag ctgagcgtat caatgaaatc tacaaacacg gtacaccgct
    10801 agaaaagaaa attgccagcg tctttttaga aacattcttg ttctattcag gtttttatac
    10861 tcctctttat tatttaggaa acaacaaatt ggctaacgta gcagagatca tcaaactaat
    10921 catccgagat gaatctgtac atggaaccta catcggttac aaattccaat taggattcaa
    10981 cgagttacct gaagcagaac aagaaacgtt aaaagactgg atgtacaatc ttctttatga
    11041 gctttacgaa aacgaagaac gttatacaga agaattatac gatccaatcg gctggacaga
    11101 agaagtcaag acattcctac gctataatgc caacaaagca ttgatgaacc tagggatgga
    11161 tccactcttt ccagatactg ccaatgatgt caacccaatc gtaatgaacg ggatttcaac
    11221 aggcacaagc aaccatgact tcttctcaca agtcggaaac ggctatttgt taggtcatgt
    11281 agaagcgatg aaagacgacg attatttgat tggattggaa taaggattta ttttaattca
    11341 aaaatttttc gaaaaataaa aagagtttag cgtacagata ttttaaatgt acactgaact
    11401 cttttttatg cttattcact tgtttcttcc agcacttttg gactgtctaa cttttccgaa
    11461 acatgttcca ttcttctcaa taaatagatt ttccggatat ttaatattat cgtcttaatc
    11521 actgcataga caggtatgca aatcagcatg cctaatattc ctgcgacact tcctgctcca
    11581 atcaatacca agatgatcgt caaaggatgg atattcattg atttcccaaa taacaacggc
    11641 ttgatcaaat ttccatctaa ttgttggatc actaaaatcg acaacgccat atacaaagct
    11701 tgaaatggag agataaacaa accgacaatg attgctggta ccgctccaat aaaagggccg
    11761 acatacggga taatattcgt gattccgcaa atgaccgcaa gcaacaagct ataaggctga
    11821 tcgaatattc tcattgccaa aaagctcatt acaccaataa tcaaagcatc taagatcgta
    11881 ctgctgatat aggcagataa tgtttcgttt aattctttta ccgtttggcg taactcttta
    11941 cgaatcgctt tcggaaagaa gtgagacaac gcatcaataa atttgtgccc atccttgaac
    12001 ataaatagca aaataaatgg aacggtaaat aacaaaataa aaaattgcat cataaatgaa
    12061 atgatttttg agacacttgc agataaactg actaatatga tattgaacaa attactaaga
    12121 gataaatttg ctgatgctaa ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc
    12181 gccaattcat ttaaccattt gcttgtttca tctgcataca ttggcaagct ctgggtcaat
    12241 tgaattgtct gttccattaa ctgaggcact acattcatga cagttaaaac aactaaaatg
    12301 attacgacca aaaacgtcaa taaaaaaccg aacactctcg ggatccgatg cttttcgaga
    12361 aaaacaacga ttggatcgaa catataatag ataaaaccag caacaagcaa tggtaaaaaa
    12421 actgccgaaa gcattttcaa tattggacta aaaatattcg gcatttgtaa aataaagtac
    12481 acgccgatga tcgtgagtac tagctccact gtccagaaaa ataattttga ttgtttaaaa
    12541 cgtgattgca tacatctcct ccaaggtcct ttgatacttc tattctatca tttaacaaag
    12601 agttacgcgc tgagattttc atttgcttaa aaaaaacaaa aaaaataaac atattttaat
    12661 gagtagacga gtacttaaac aaaaacacgg tttggagtag tgacaaaaat tatactgcag
    12721 tccctttttc caaataaacg gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa
    12781 cgaaaaatat aaggaactat ttttatttgg ctgggcttat tacttgatgt aggacatcta
    12841 ttttacaacc tcagatcatt tattgcgcaa tactgactaa atgcaacttt gtgatatttt
    12901 tgtgtacgga caactcctcg atcacatcgg cataagtgag cccttttgga agatcaatcg
    12961 tatagatatt tttgtacact cggtagtcat caacgaattt tacgtcaaaa ttcacatctt
    13021 cgattacgat atttttctct tcaaaataat cggtaagaaa atctttggtt tcttttctat
    13081 ggataaattg gatttctaac tttttagtcg taggtacatg gatcacgcgt ttcactactg
    13141 tgagtgcaat agcaatcccg ataaaactga tgatcgcaat ggaatagtaa cccatgccga
    13201 tggcgatccc taatccagca attgcccata aagaagcggc agtcgtaagt cccgtcaccg
    13261 attgtttcgt gacgatgatc gttcccgcgc ctaagaaacc gacaccactc accacttgag
    13321 caatcagcct tgcttcatct gaacgaatcg ttccagcaag ctgtggatac tctaaagcat
    13381 ctcttaatgc accactagcg atctcaactt ggatcaatgc aatgatacat gctcccatac
    13441 aaactaaaat atgcgtacgc atacctgctg gacgattttt atattgtcgt tcaaaaccga
    13501 ttgctccgcc aaatagcata gcgatcccta aacgtaaaat aatctctgga attgtcaata
    13561 ccatatcatt catctaaaca ccctccagtc caatatatca tgtctgaaag gagcagacaa
    13621 aaaaagtgca atttttacct cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc
    13681 ctttttatga ataaacggtg ttcaatcagc tgaccctcgg tattactcaa caaacggcaa
    13741 aatatgggga gctatttttc ctttgtcttc tctaatactc aggtcaaaac gctgatttca
    13801 caacctcttt agcctactat tgaaaattac aacttacttg ctgcttcttc atcgatgatc
    13861 acaacaacat tgtcgtgatt ttgcaataca cttgctggca aatgattcgt tacaggtcct
    13921 tcaatcatcc ctttgattgc atcagctttg ctttcgccaa atgccatcaa gacgatttct
    13981 ttacttttca taattgaacc gattcccatt gaaacagctt ttgttggtac atcttctgct
    14041 ttatcgaaga atcgtttgtt tgcattgatt gttgattctg ttaagtcaac tactgaagtc
    14101 gttccgtcaa atgaagcacc aggttcattg aaaccgatat gtccattttg cccgattcct
    14161 aagatttgga tatcgattgg atgtgcatcg atgatttgat catacttttt gcatgctgct
    14221 tccagatctt ctgcttttcc atctggtaca aacgtttctt tgaatggttt tttatcaaat
    14281 aattggacat ccatgaaatg acggtagctt tgttcatctg aaccgcctag tccaacgtac
    14341 tcatctaagt ttacagaagt catttcactg aaatccaaat cgcttgaagt catttcttta
    14401 tataaagtaa ctggtgtgct tcctgttgcc aatcctaata cttttgcacc tttgttcata
    14461 ccttctttga tgatttcaaa tgcttttttt ccgccttctt cagcgttttt tacacgaatg
    14521 atttccatag tgattgctcc tttatatttt ggtatagccc aattatagca ttcttttttt
    14581 ctttttacaa taattagtat agaccaaatt gcattttttt cattttcttg acaataaata
    14641 ggaatccgtg ttaggtttca taagatggtt caatcaaaca atccatgttg catgaaaacc
    14701 aagcaagtga ttcactgtgg acgctttatg tctgcagttt ttttatgaaa aaggagttat
    14761 tatgagtata ctgacattaa acaacattac ccagcaattt ggcgacaaaa tcctctatga
    14821 aggtgtcgat ctgcaattaa atgctggcga acatcttggg ctgattggac aaaacggagc
    14881 cggtaaaagt acattgatta aaatcatcac tggtgagatc ttaccagatg acggacagat
    14941 ccattggcaa aaaaatattc acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac
    15001 attaacaatc gaagaatttc taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa
    15061 aattgctttt ctttaccaaa catatagcga gaccttcgat gattccttgc tggaaaaagc
    15121 tgggaaactc caaacggaat tagaccaagg agtgttttat caaatcgaca ccttagttgc
    15181 tgagatgagc agcgggttgg ggatcgatgt cttaggttta gattcactgt tgaaaaatct
    15241 aagtggtgga caacgttcaa aggtgatttt agcaaaacta cttttagaaa acccagatgt
    15301 tctgatctta gatgaaccaa ccaatcactt agatgatcag catatccaat ggctgacgca
    15361 gtttcttcag gattttgacg gtgcgtatat cgtgatttcc catgaccaag aatttcttga
    15421 tcagatcacc acccatatcg cagacatcga gtttggaaag attactaagt atactggcca
    15481 tctaaaacaa gcattgaaac aaaaggaaca aaatcgcgaa agctatttgc gccaatatca
    15541 tgctcaacaa aaacaaatcg aaaagaccga agcttatatt cgaaaataca aagccggttc
    15601 ccgatcaacg atggcaaaaa gccgacaaaa acaattagat aaaatcgaac ggctgacgcc
    15661 tccagcttca gcaagtaaag ctgtctttga ttttccattc tcgcctattg tcacgacatt
    15721 agcagtggaa acaactgatt tagtcatcgg atatgatcga ccgctacttg cacccatcaa
    15781 tttgaccatc cgctttggtg aaaatattgc cattcgaggc ttcaacggga ttggaaaatc
    15841 tactttatta aaaacactga tcggtgagat tgaaaaaata agtggcgatt tccacttccc
    15901 agataacaca aaaatcaatt acttttcaca agatctgtac tgggaaaatc cattagaaac
    15961 accactacaa tatttaagca gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca
    16021 actagctaaa gcagggttag ttaatcaatt agcaagtgaa cctctcacta cattaagtgg
    16081 tggggaacaa actaaggtga aacttgcaca gttgacaatg aaccaaggta atttactgat
    16141 tctcgatgaa ccgacgaacc atatcgatca agaaacgaaa gaaagcttac aagacgggat
    16201 acagaaattt ccaggaacag taatcatcgt ctctcatgaa caagaatttt atcaagatct
    16261 tgtgcaacga gtcattgaga ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa
    16321 ggagctgtga caaaagtttt gtcacaactc cttcttctga ataaatggtg gccaattagc
    16381 tgaccttcgg tattagctca aaaaacggca aaatatgagg agctattttt atttggctat
    16441 tcttattact tgatgcagaa cagcttttca caacctcttt tcgtctattt tttattttcg
    16501 agatttctct ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca
    16561 agcccgctaa ccgttacacc taaccagcca tactgctgcc acattgtcgt ccctagcaat
    16621 gaaccagccg cacctccaat aaagtacata aacataaata cagtattatt tcggttgctt
    16681 gctgcttcac ctagcccttg tactcgtgtt tgattggcga cttgaccaaa ttgattcccg
    16741 acgtctaata aaataatagc taaaagcaaa atggccaaat ggcttccgcc aaccaataaa
    16801 gcaataaagc ttattgtttg cataacaagt cccatataca cgatttttct ttcagaataa
    16861 gaatctgcca gtcgtcccac gataggtgca gcaactgctc cagacaaacc aaaaatcgct
    16921 aatatccctg cttcgaatgt tccccaattg taggcgggac tgctgatata aaaaatcaac
    16981 gtagaccaaa agatcgaaaa tgttccaaac atgaaaaatc cactgactgc cgcttctctt
    17041 aatagacgtt gagaagcaac taaatgcggc aaacttttaa gtgaatcaag gtatgaaaca
    17101 gctgtactac tttgctggac agaacgcggc attttccagt aaagcagaag tgtaagaatt
    17161 ccgactgcac ataaagcaaa taaatagatc atccgccaag aaaaaagact ggcaacaata
    17221 ccagataccg tccgagaaag caaaatcccc acgagtaaac cgctaagcaa tgtacccatg
    17281 accttccctc ttgcttttgg tcccgctagt accgcaccgt aaggaatgat gatttgcggc
    17341 acgatcgaaa gtaatcctat taaaaaagaa gcgcatgcaa ataacgtaaa cgaaggcgca
    17401 aaaaaagcgg ccaacagaga gacggcagat aacgctgcca ttcgaatgat caattttgga
    17461 cgatccacca cgtcccctaa aggaactaaa aacagtaacc ctaacgcata gcctaattgg
    17521 gtcaacatcg tcaatatccc gactgcactt gtgctaacag ataaactagc tgctactttt
    17581 gttccaatcg gctggatgta atacatattg gcaacaacaa cgccacaagt gattgataat
    17641 agtaaaataa gcgaagttgt taaaggtttt ttttcattac tcttcattga tttcccccat
    17701 ttcctatctt agtttacaac ttatggaaaa gattggaaat agaaaaatac aactattcgc
    17761 cacacttatt atttgtgctt ttgttttcct tgttttttct tgcgatcttt ataaatgaag
    17821 acaactaacc ctactagcac agtaacactt gcaaccaacg tgactgcagt tttgttatat
    17881 ccgtgaaaca gagcttctcc accacacgca tacaacacag acgaagggat gactccaatc
    17941 acgatcgaca atatgatttg ctgtagaggc aatttcatcg catctgcagc aaaattgacc
    18001 atagaagatg gaataaccgg tatcatatag cctaacatta cacctatttt tggatgtttc
    18061 atttgtcgga ttgcttgcac ccaatgattc gtttcggttt tcttatctaa aaattttaag
    18121 cgatgcataa ggaaaattgc agtgatgtta ccaagagcat tacccaaaat gttgatgatc
    18181 gcacctgtca gtggtccaaa gctcaaacca gcaatgactc caactactga agtaggaata
    18241 cctggaacag cacacatcac cgctgtcaat acgatcaaga gtatcgatgc aaaagtccca
    18301 tgcgaacgaa tatgtgcgag tagctctgcc ttactcgctc tcggatttaa aaataattgg
    18361 atgtctgtct gatattctaa atacaaccga tagcccacta ccagtatcag aatgattccc
    18421 aacgagatca atatcaatcg gattattttc tttttcatat ttcctcctga aaaaaatcta
    18481 tattcattaa ataatagttt aacataaaaa caaaaaagca ccgaaatcca acataggatc
    18541 tcagtgcttt taaatagtaa aaattattta acagttacag atgcgccaac ttcttcaagt
    18601 ttagctttca attcttcagc ttcttcttta gatacgcctt ctttaactgg tgcaggagcg
    18661 ccatcaacta cagctttagc ttctttcaag cctaaaccag ttgcttcacg aactgctttg
    18721 ataactttaa ctttttggtc tccagctgca gttaattcta cagtgaattc tgtttgttct
    18781 tcagcagcag cagcgccacc agcagcagct acagctacag gagcagcagc agatacgcca
    18841 aattcttctt caatagcttt aactaggtcg ttaagttcta ggatagttgc ttcttttaac
    18901 tcagcaacga tgttttcaat gttcaatgcc attataattt cctccgttta attgttcttt
    18961 tttatttaat gctttatgcg gcaattaagc tgcatctcca tcttctttgt ctgccactgc
    19021 tttgacagcg taagccacgt tgcggactgg cgcttgtagt acagatagca acatagatag
    19081 aagtccttcg cgatttggca attttgccaa tgctgtgatt tcttccaatg aagagacttt
    19141 accttcgatg ataccgcctt tgatttctaa tgcttttgct tctttagcaa attcgtccat
    19201 gattttcgct ggtgcgacta catcttcgtt actgaatgca acagctgtag gtccagtgaa
    19261 tacttcgtcc atgccttcaa ggccagcttt tttagctgca cgtgaaagaa tagaattttt
    19321 gataactttc atttctacgt tagcatcacg aagttgttta cgtaaacgtg ttacttcatc
    19381 aactgttaat ccgcggtagt caacgactac tactgatgca gctgctgtaa atttttcgct
    19441 tacttcatca acgattgctg ctttttttgc gataattgct tcactcattt ttgagtttca
    19501 cctcctgaat tttgatggaa gagttttttg cactgaaaag gcaagtcact tttatagtta
    19561 ctttgctcaa ccagtattat tcatcaccta ttcacttcgt tcatagtgat tctactcgct
    19621 ttgctcgaaa ggaaactcct ttcgctttgc tcaaccagta ttgttcatca tctcttcact
    19681 tcgttcatag tgattcacaa taaaaaactc tatgccaccg cagacataga gggactattg
    19741 aatatattca ataaacgtcc tcggtaggaa attaaggctt gatgccacct actgtcttcg
    19801 gtacaagtta attattaacc tcaactagct tagcatgcta aactagttga gtcaagaatt
    19861 atttttcagt tggatcaatc aacattctaa tatttaatta gaaagaagct tggtcaacat
    19921 ggataccagg tccaaatgtt gttgtgatcg ttacattttt catgtattga cctttagctg
    19981 ctgatggttt agcttttagc aatacatcat ggattgctgc aaagttttca actaattttt
    20041 catcgtcaaa tgaaactttt ccgattggta catggatatt tccagctttg tccacacggt
    20101 aagttacttt accagctttc acttcgttta ctgcttttgt tacgtccatt gtaactgtac
    20161 cagttttagg gtttggcatt aagccttttg gtcctaatac tcgtcctaga cgacctacag
    20221 tagccatcat atcaggtgtt gcaacaacta catcaaagtc aaaccatccg ttttggattt
    20281 tttgaaccat gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct tcttttgctt
    20341 tgtctccttt agcaaaaact aaaacagttt gtgttttacc agttccgttt ggtaatacaa
    20401 cagcaccacg gatttgttgg tctgcttttt tagggtctac gttcaaacgg tatgcaactt
    20461 caactgttgc atcaaatttt gcaatgtttg tttcttttgc taaagctact gcttcggcaa
    20521 caggataaac tttacttgaa tcaacttttt ttaatgcatc ttgcattttt ttgctctttt
    20581 tagccatttt ggttcctcct tgattgtggt tataacggtt gtacctccca cgtgttctat
    20641 atctttcaat atagagccaa ctgagaagct gctgtcttag ggtgtataga ttattctaca
    20701 gtgatcccca tgcttcgtgc agttccttca accatgcgca ttgctgcttc aacgttagct
    20761 gcgtttaggt cttccatttt taattcagcg atttctctta cttgatcgct agaaactttc
    20821 gcaactttat ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc ttttttcaat
    20881 agaacagcag ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc gtatacagaa
    20941 ataacaaccg gaatgatcaa acctgcttga tcagctgtac gagcgttgaa ttcttttgtg
    21001 aatcccatga tattgatacc cgcttgacct agtgcaggac ctactggtgg agctggagtt
    21061 gctttacctg caggaatttg caatttaacg attttttcta ctttctttgc cacgagacat
    21121 acctccttaa gtccgtgatg tggttaattg gagatgcata tttctcctcc cacgttttat
    21181 ctgatctgaa aaaatccaga caaataatgt gcaaaacaat gcacactggt acagtatagc
    21241 aaatttattt gttttttcaa gatttttttc tgctattttt acattttgct tgaataaacg
    21301 ctaaatttat tctaaaaaaa caacaatttt tcgttctcat ttcttctttt atgttacact
    21361 tgcgctagca tggttcaaca gatgaaagca aacaataaca cggtttttta gacccttgta
    21421 caaagattcc tacataacaa aatgaggtgg agaaaatgaa agatttaagg tatagaagtg
    21481 tctttgatat tattggacct gtgatgattg gtccaagtag ctctcacact gccggagctg
    21541 ctagaatcgg gaaaatcata cgtagtatat ttggcgaaca gcctgattct gtagatattt
    21601 atctatatga atcatttgca aaaacctatc gggggcacgg aacagatatt gcattagttg
    21661 gcggcctgtt agggatggaa cccgatgatg aacgtttagc tgattctttg aaattggcat
    21721 atgaagccgg tatggaagtc ttgtttgttc ctaagagtga aaaagccgaa catccaaact
    21781 ctgtaaagat gcttctaaaa aaaggaacac gaaagctttc agtcactggc atttcgatcg
    21841 gcggtggcaa tatccagatt tctgaattga atggattcaa aatttcttta agcatgggta
    21901 ctcctacctt gatcgttgtc caccaagatg ttccaggtat gattgctaaa gtgactagta
    21961 tactttctga gaccaatacg aatatcggca ctatgacagt gacacgggaa tcaaaaggtg
    22021 aaaatgccat tatgataatt gaagtagatg atcctcaagt agaagaaacg gtaaaaagac
    22081 tcaaacagct gccaaatatc gacagcgtaa attatttcga ttaaggagct aaaaaaatgt
    22141 ttttatcgat tgaagaatta gtagatcaag caaaagctta tccaagtgta gcagaattga
    22201 tgattgcagt tgaaatggaa atgagtggcc gttcccgtag tcacatcatc gagatcatgg
    22261 aaaaaaacct ctcagtaatg gaacaatcaa tcaacgaggg aaaagaaggt gttacctccg
    22321 tcacaggaat taccggtggt gatgcaaaaa aattagatga ttatttaaat gaggcagact
    22381 ttttaagtgg agaaacaatc ttaaaagctg tacgcaatgc tgtagctgtt aatgaagtca
    22441 atgcaaaaat ggggttgatt tgtgctacac cgactgccgg aagcgcgggt gttgttcctg
    22501 gtgtgctgat ggctgtccga gatcggcttg atttgacaca tgaacagcaa ttggaattct
    22561 tatttaccgc tggcgctttc gggttagtca tcgcaaacaa cgcttctatc agtggtgcag
    22621 aagggggatg tcaagcagaa gttggttctg caagtgcaat ggcagcagca gctttggttt
    22681 gtgcaaaaaa tgggactcct catcaagcag cacaagcagt agccattaca ttgaaaaata
    22741 tgatgggact gatttgcgat cccgttgcag gcttagttga agtcccgtgt gtcaaacgta
    22801 atgctttagg ctcttctcaa gcttttatct ctgctgatat ggcattagcg ggtatcgaaa
    22861 gtgtcattcc gccggatgaa gtcgttgctg caatgtacca agtcggacgt caaatgccgt
    22921 cgattttcaa agaaacagca gaaggtggct tagctgttac gccaactgct aaacgacttg
    22981 cggaagagat tttaaataag agggcgtgat aaagctcctt aatttcgagt attaagagat
    23041 tagttatctt cataacaaaa aacgaagaga ggactaattg cccttctctt cgttttttat
    23101 taatccatca atttcgtatt ttttacttca tcgatcgttt ttgttcctgc aagttgcatc
    23161 gtgatcgaca attctttgtt caggtgttca aaaacagatg ttactccttc tgccccacct
    23221 agattcaacc cataaatcac tggtcggcca atcgcaacta agtcagcacc acttgccaga
    23281 gctttgaaga catgctctcc tcgacggaca ccagagtcga aaatcactgg tacacgtttg
    23341 ttgactactt cggcaatttt tggcaatact tcaaaggacg caggacctcc atctagttga
    23401 cgtccaccat gattggatac ccagatacca tctgcccctg cagagatagc gattactgca
    23461 tcttctggcg attgtattcc ttttacgata actggaagat gggtgatttc tttgattgtt
    23521 ttgatgtcat ctggtgtcaa gccttgtttt gcagctgcat aaatttctgc gattcctttt
    23581 ccttcgccat taccacttgc actttgttcg ctatacgcag ctaagttagg cattggtaat
    23641 ggaaattgga attgattgat gacgtcttct tcacggtaac caccgagcgt tgaatctgcc
    23701 gttaaaatga tagctttagc ccctgcttct actgctttgt tcaaaatgaa ctcattgaat
    23761 ctgtcgtctt tactcatata taattggaaa aactgcggtg cgcctggcgc tgcatcggct
    23821 gcgtctttga ttgtcgtatt tgcataagtg ctgatggaaa agattgatcc agcagcagct
    23881 actcctttag ctgtatcagc ttcccctttt tcatgtgcca atccttgggc cgctgatggc
    23941 gcttggataa tcggtgtatc taattcaatt ccgaaaacag atgtatgcaa atcggctgag
    24001 tcgattccgc gtaatatccg tggcatgatt ttctttgtat taaaagaagt cgtattttct
    24061 ttcatcgtcc actcatcttc agaaccaccg cgaatatagc caaaagctcc tgcttccatg
    24121 cgctctttta cacgaccttc tagttaggca atgtttacaa tatcgattgg atgttcttca
    24181 ttacttgtat gataagtcat agtagatcct tctttcatct tcttgattac atataaatca
    24241 tactaaactt acaaaaagta agcaaacatt tactctcaga tacttttatc gctattcttt
    24301 ccaaataaat cgacttacaa gctgatctac ttcttggttt tcatgaagca tactatgaga
    24361 ggcattttca cctgtcacta ctttttcctc tatttcattt ccgcgttgct tcaacaacgc
    24421 atagaccgct aatgcactgt ttaacggtac tgttccatca ctcaagtctg tttcatctag
    24481 ttgaccagca atcaagaaaa atcgagtggt tataggaata ttaccgatca tttgctggta
    24541 atcttgatag cggttagaaa cgacagcagg cccctttgct aactcatctg tcagagactg
    24601 cgcactatca tcgacaaaat cattaaaagg cgcgccaatt gctgttaatg tctgaacttg
    24661 tggcaatgac gtatcctgtc catacgttcc taaatagcgt agtgcactaa ctcctcccat
    24721 ggaatgcccc actatgttga ctttatctat gccgtagttt tctttcagat acaaaagaca
    24781 agttttgatc cattctgctt gattccactc gttattttta ttatcttcaa acaatacttg
    24841 gaccatcgga ttgtctgctt ttccagacaa atctcctgtt gcttgtattt gtccatctgt
    24901 tcctactgtt aaaaccaatt cttttactgc ttgctgattt ttttccaaac gccgcaacat
    24961 ccctttaaag gaattttctg tcccgctgta gccgtggaaa aaaagtgttg gaatagtggt
    25021 tttttctatc actttactgg attctgccgt tttttcttta tcagaaactt cagtagtatt
    25081 tatcttcgtc gtctggcaac cacttaacgc aaacaaactg ataaggacta aagctgctgc
    25141 agttgcacgt ttgcataaat ttttgattgg ttctgtcata cacctcttcc tttccatata
    25201 gaaaaaagaa agacttgaaa ggccaatcat atgatcgaca tttcaagtct ctttattaat
    25261 cgattttatc tacttgatcg aaatcaagtt ctgtactggt ttcacgtcca aacatatcga
    25321 tgttgacttt caatttttct ttttctccgt caacttctgt tacttgtcct tctaatccag
    25381 caaatgcgcc ttcgatgatt ttcacgactt cgccaacttc tacttctaga tcattttgtc
    25441 gcaggctcat accgatagaa cgcaagatat gattgacttc ttctggaagt aatggtgctg
    25501 gtttgcttcc ggctccgtgt gaaccaacga atcctgtcac accaggtgtg ttgcggacaa
    25561 cataccatga agcgtctgtc atgatcattt caacaagtac gtatcctggg aatgttttat
    25621 gaacgatttc tttttctttc ccatttttga cttcagtttc tttttcttca ggaacaacga
    25681 cacggaaaat aaagtcggcc attttcatac tttgcgcacg agattcgata tttgctttta
    25741 ctttgttttc atatcctgaa tatgtatgca agacatacca gtttttttca aaagattcca
    25801 ttgaaggctc ctcatttcat tgtaaaataa aaaaacctca gtacacccga agtttttctt
    25861 tcgcttcatt atagcactaa aagtacgttg tggctagcga gttattttaa aatccagcca
    25921 aataagcttt ggatggctgt atccattacg taaaacatca atccgaaaat aatagaagtt
    25981 tcgatgacta ctaacgtatc tttacggagt tgttttctgc ttggccaagt tacttgtttg
    26041 atttcatctt ttacgctttt tagaaatttc atgagacaac ctcccttgct actgtttatt
    26101 tcgtttcttt atgtaatgta tattgattac aatatttaca aaacttattg atttccaaac
    26161 gttcgccgct ttttccttca ctgactgatt ttgtgtagtt gcgagaccca caaacggaac
    26221 aagcgagtgc tgcttttttc tttgccattt acttctgccc ccttgctgag aatcagactt
    26281 atacttcata acagtatcac gtaacttttt acttgtcaat ttgtgaaata aactatctat
    26341 tgttttggat aaaattatga tatgattaat ttgaaattaa gctaaaatga gacaattgga
    26401 ggtatagcga tgctattaaa aacacttgta tataagaaaa aagatctgac aaccgttaca
    26461 gagactagta cattagaaga agcattaaaa attttagaaa cttccggata ccgttgtgtg
    26521 ccgatcttag attcttcagg taatattttc cgtggaaaca tttacaaaat gcatatctat
    26581 cgtcataaag caaatggcgg tgatatgacg cttcctgtca ctcatctttt gaaaaacgct
    26641 acaaagttca tttacctgga tacttctttc ttcaaagtat tctttacgat caaagagctt
    26701 ccttatattg ctgtccttga cagtgaaaat catttttatg gtattttgac acatagtacg
    26761 ttgcttaata tgctctctca atcttggagc gtagaacaag gaagctatgt actgacaatt
    26821 gcttccacag gaaagcaagg agatttggct gctattgcaa aaatcatttc aaaacacagc
    26881 agcattgcca gctgtattac tcttgatatc gaaaaagacg aatacattcg acgtacattg
    26941 atcacgctac cggctaacac tgaagcgaaa gtatgtgaaa cgatcgtcaa ccacttagag
    27001 aaaaagaatt ataaagtggt agaaattgaa aatttgcaaa accaagaagc ataatcaaaa
    27061 agcgaaaaaa gaaaacagct aatctgcttt cttttttcgc tttttataat tagacacgct
    27121 tttcaaaatc aagcgcgcga tctaaataat acatcaatgg tgctgaaaca gccatgacga
    27181 taaactcact taatgcaagc gtgccatagg ttgtccaaaa tgcttggcca cctgttggaa
    27241 ctaacatata agcaatcagt cccatgctga tggtaaagaa gatcgtgttc aatactaaac
    27301 gcatctttac atttttcact ttgttttgca ataaagctgt caggcctaaa gaaagcaatg
    27361 tttggccgcc accaaaaacc acatccatga taccaaaacc aaagaaggca ttatagacga
    27421 tgaccccgcc taaaactccc caaagcaatt tacggttaaa gacaactaaa tgattcaaac
    27481 tttctgaaat acgaaactgg atcggccctg aggatacagg cgcaactaaa atagttaacg
    27541 ctagataaag agccatgatg atgccattta caacgattac ggataaccgt tgtctgcttt
    27601 tcgttttttg cattttctct cctcttttct cctagttttt ttaacgcggg atggttgatg
    27661 aaccgcggtt atgtgaaaca aacaataatt ataacagact tttcatttat ttcaatccgt
    27721 cttatctgct tttaaaaaag caaaagctta aacaatgtaa acgtttgcta aaaatcgtgg
    27781 gatatggtat aaagtaagtg taaggggtca taagaattcc cagtaaagca gacgaagtag
    27841 gatctgcaag aacagaattg ttgtgaaact taaataaata aaaaaggatg tgggaatttg
    27901 agcaacctct tactagagaa cggtctagca agcagctgaa gatctaagtg gacatgccga
    27961 accttggaac ttctccttac gtaagtaatg gatcatgcgc ttgctaggct
//
LOCUS       DAZUMT010000025        27617 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000025 DAZUMT010000000
VERSION     DAZUMT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.025"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..639)
                     /locus_tag="ACG8FM_001019"
     CDS             complement(277..639)
                     /locus_tag="ACG8FM_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093698.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(713..1093)
                     /locus_tag="ACG8FM_001020"
     CDS             complement(713..1093)
                     /locus_tag="ACG8FM_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS5093699.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1175..2350
                     /locus_tag="ACG8FM_001021"
     CDS             1175..2350
                     /locus_tag="ACG8FM_001021"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS5093700.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2427..3011)
                     /locus_tag="ACG8FM_001022"
     CDS             complement(2427..3011)
                     /locus_tag="ACG8FM_001022"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5093701.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3212..4210
                     /locus_tag="ACG8FM_001023"
     CDS             3212..4210
                     /locus_tag="ACG8FM_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5093702.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4322..4831
                     /locus_tag="ACG8FM_001024"
     CDS             4322..4831
                     /locus_tag="ACG8FM_001024"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS5093703.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4861..5520)
                     /locus_tag="ACG8FM_001025"
     CDS             complement(4861..5520)
                     /locus_tag="ACG8FM_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS5093704.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5522..6286)
                     /locus_tag="ACG8FM_001026"
     CDS             complement(5522..6286)
                     /locus_tag="ACG8FM_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS5093705.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6294..6971)
                     /locus_tag="ACG8FM_001027"
     CDS             complement(6294..6971)
                     /locus_tag="ACG8FM_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS5093706.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7008..8393)
                     /locus_tag="ACG8FM_001028"
     CDS             complement(7008..8393)
                     /locus_tag="ACG8FM_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS5093707.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8603..8687)
                     /gene="ffs"
                     /locus_tag="ACG8FM_001029"
     ncRNA           complement(8603..8687)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8FM_001029"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="ACG8FM_001030"
     CDS             complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="ACG8FM_001030"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS5093708.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9332..10189
                     /locus_tag="ACG8FM_001031"
     CDS             9332..10189
                     /locus_tag="ACG8FM_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093709.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10270..10605)
                     /locus_tag="ACG8FM_001032"
     CDS             complement(10270..10605)
                     /locus_tag="ACG8FM_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093710.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10672..12042)
                     /locus_tag="ACG8FM_001033"
     CDS             complement(10672..12042)
                     /locus_tag="ACG8FM_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS5093711.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12120..12656)
                     /locus_tag="ACG8FM_001034"
     CDS             complement(12120..12656)
                     /locus_tag="ACG8FM_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5093712.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12965..14446
                     /locus_tag="ACG8FM_001035"
     CDS             12965..14446
                     /locus_tag="ACG8FM_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093713.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14549..15256)
                     /locus_tag="ACG8FM_001036"
     CDS             complement(14549..15256)
                     /locus_tag="ACG8FM_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS5093714.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15335..15673)
                     /locus_tag="ACG8FM_001037"
     CDS             complement(15335..15673)
                     /locus_tag="ACG8FM_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS5093715.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15784..16746)
                     /locus_tag="ACG8FM_001038"
     CDS             complement(15784..16746)
                     /locus_tag="ACG8FM_001038"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS5093716.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16761..18092)
                     /locus_tag="ACG8FM_001039"
     CDS             complement(16761..18092)
                     /locus_tag="ACG8FM_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS5093717.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18100..18780)
                     /locus_tag="ACG8FM_001040"
     CDS             complement(18100..18780)
                     /locus_tag="ACG8FM_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS5093718.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(18999..19478)
                     /locus_tag="ACG8FM_001041"
     CDS             complement(18999..19478)
                     /locus_tag="ACG8FM_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS5093719.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19605..21284)
                     /locus_tag="ACG8FM_001042"
     CDS             complement(19605..21284)
                     /locus_tag="ACG8FM_001042"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5093720.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21832..23334
                     /locus_tag="ACG8FM_001043"
     CDS             21832..23334
                     /locus_tag="ACG8FM_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093721.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23347..24009
                     /locus_tag="ACG8FM_001044"
     CDS             23347..24009
                     /locus_tag="ACG8FM_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093722.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24110..24313)
                     /locus_tag="ACG8FM_001045"
     CDS             complement(24110..24313)
                     /locus_tag="ACG8FM_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5093723.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24424..25608)
                     /gene="iadA"
                     /locus_tag="ACG8FM_001046"
     CDS             complement(24424..25608)
                     /gene="iadA"
                     /locus_tag="ACG8FM_001046"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS5093724.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25765..27504)
                     /gene="yfcC"
                     /locus_tag="ACG8FM_001047"
     CDS             complement(25765..27504)
                     /gene="yfcC"
                     /locus_tag="ACG8FM_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS5093725.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8226 a         5766 c         4673 g         8952 t
ORIGIN      
        1 tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt tatcatgtat
       61 ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt cgatcaatta
      121 ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca ctaaaaaact
      181 atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa
      241 aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac
      301 ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc
      361 taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc
      421 tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat
      481 ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa
      541 cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa
      601 atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt
      661 gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct
      721 tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg
      781 caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg
      841 gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt
      901 catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat
      961 ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa
     1021 gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa
     1081 ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa
     1141 aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc
     1201 aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga
     1261 cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc
     1321 gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata
     1381 tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat
     1441 cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc
     1501 aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac
     1561 aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga
     1621 cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa
     1681 tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt
     1741 gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt
     1801 cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc
     1861 ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat
     1921 caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga
     1981 aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt
     2041 aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca
     2101 gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt
     2161 taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt
     2221 cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat
     2281 tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac
     2341 aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt tcgtcctttt
     2401 tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc aatcactaca
     2461 tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc tactgtgtcc
     2521 atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg agttccgcca
     2581 cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg tccaagcata
     2641 ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt gtttggacct
     2701 gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa ttcatcttca
     2761 aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc gccgccttgg
     2821 atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc ttttttggaa
     2881 agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg aacagtaatg
     2941 tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa atctagttgt
     3001 gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa taacataata
     3061 cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac tagatacatt
     3121 ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc tcaattaaat
     3181 tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta caatcatcgg
     3241 tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag ctaaaacaaa
     3301 aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc ccgaaaaata
     3361 tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg atcaattaga
     3421 aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc tctcgcttac
     3481 tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt ccaaagcagt
     3541 cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag ataatgctga
     3601 atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt atgctggaaa
     3661 aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga tgttagagcc
     3721 tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc acgaacatag
     3781 tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca tcgatggatt
     3841 atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa gtgacgagac
     3901 gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta atttagaaca
     3961 tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat cagatatgtc
     4021 tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag gaaaagtgaa
     4081 actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt tacactatat
     4141 taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata aaaatcttcg
     4201 tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc agtttcttgc
     4261 atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg ggagcaataa
     4321 tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga aggaacgtgg
     4381 cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact atatttctga
     4441 tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc gtgaagtaca
     4501 taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa aattgatgag
     4561 tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg agatcctagc
     4621 cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt atatcgacaa
     4681 ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg tccatacctt
     4741 cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag cacacaaaga
     4801 accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga aaacgtctat
     4861 ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga aaagaaaaac
     4921 agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga aaaattcttc
     4981 tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat cgttgttgaa
     5041 aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga aaaaaccgaa
     5101 aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa gccgatcatt
     5161 cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa acacagcata
     5221 atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag cagaaactgg
     5281 gaaatctggc aagctgagga tggttttgaa gggattattg agataattca ttaggtgatc
     5341 aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt gcaaatgtcc
     5401 gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca gactgatcag
     5461 tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg aatttttcat
     5521 attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata ggcaacccag
     5581 gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata ttattgcgta
     5641 tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct tctttttgca
     5701 gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg atgtagatcg
     5761 gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca ggaagaaggc
     5821 cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca cctttttgaa
     5881 tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag cctaccacta
     5941 catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag tcaatcaatc
     6001 ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata aaatcgattg
     6061 ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac tcatctgcta
     6121 gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac aagaaaggta
     6181 attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt tgtgtcccac
     6241 gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat ccactactct
     6301 tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg ttgttcttgt
     6361 ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat aaccggtttt
     6421 ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt atggtttttt
     6481 ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc gatgacaaaa
     6541 tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc gattcgctga
     6601 tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag ttgttcataa
     6661 ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg ttctccaagc
     6721 ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct ttcacctatc
     6781 aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc tgctttaggc
     6841 tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt ttcctgttca
     6901 gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg ttcattttgt
     6961 tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta tttgattggg
     7021 tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa caaaaattca
     7081 ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg atctacggta
     7141 gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac ttgatggtta
     7201 atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc gcgaaatccc
     7261 attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc taaaactaag
     7321 cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg cggcaacgca
     7381 gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa cagaccactg
     7441 tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc tacttggata
     7501 aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc tacttctttt
     7561 tcttttaata attcatgccc acgttctaga taactattta tttcttgttc atcttctgta
     7621 aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat ttttttctct
     7681 tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc ggctgctatt
     7741 tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga tcctaaaata
     7801 acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc gagatgactc
     7861 atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg caaaatatca
     7921 taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg agcagtaaat
     7981 gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg tttcgtccat
     8041 gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc aaactcagca
     8101 tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt gtttcctatc
     8161 gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc attcgttgct
     8221 tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat cgttgtcgtt
     8281 ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat ccttggccaa
     8341 ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc catgtaccct
     8401 ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg aaacaagctc
     8461 tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc gataaatcag
     8521 tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa aaaaacagac
     8581 ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc ttagtgctgc
     8641 ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt cggcaaagaa
     8701 tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt ttcgctggcg
     8761 gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa tgccaccttc
     8821 tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg taccggctgt
     8881 tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca aaatcatcgc
     8941 gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt ccaagcgcca
     9001 gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg tagtagcatc
     9061 ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca atacaaccac
     9121 tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca atgcaaggcg
     9181 catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc cttccctgcc
     9241 tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa cttttttttc
     9301 tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac ttgctcttta
     9361 cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt cttttgcaat
     9421 tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa ccagcctttt
     9481 gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc actatctttt
     9541 taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc acgtcgaatc
     9601 aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat ttcgctattc
     9661 tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa taaaccgtga
     9721 ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg attttgatac
     9781 gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg tccctttgtt
     9841 tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc acagccgtac
     9901 tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa aagatagtaa
     9961 gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga tcattcaaga
    10021 attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc ttcatcgtaa
    10081 tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc taaaaaaagc
    10141 caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa gatagattaa
    10201 aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt gttcaaaagc
    10261 tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta catcttcggt
    10321 tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt tgatttttgc
    10381 taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga tttctagtaa
    10441 tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc gagtagtcat
    10501 atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta cactggcagc
    10561 ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta tcagctcctt
    10621 tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca ctcagtgaaa
    10681 tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc caaatccgac
    10741 aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt gaaatgtttc
    10801 tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta tattctctat
    10861 cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt tgaaatctcc
    10921 taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta actcaggaac
    10981 ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat gaaaatcatt
    11041 tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac ttttttttcg
    11101 tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg caacaaaatg
    11161 tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac cagctacgct
    11221 tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct caatcgttac
    11281 tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg caaacgtaca
    11341 agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag acaaaatctc
    11401 ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta ttgcatttgg
    11461 atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc gatcaaaaaa
    11521 gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg cgttcgaaac
    11581 tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga tctcattcgt
    11641 cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac caccgggagc
    11701 cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc caacgatcat
    11761 tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact ttccattgac
    11821 agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc tatggtaagt
    11881 ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt ttttcacgct
    11941 tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa gacgatattt
    12001 tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt ttcgttagac
    12061 attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca tgttgctgcc
    12121 taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc gtttttatcc
    12181 caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg caaacttccc
    12241 aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt gtgacctttg
    12301 acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga ttttgcaatg
    12361 atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac aatgcttaaa
    12421 acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat ttcttctaat
    12481 atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta tgtagaacaa
    12541 ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc ttccacgtat
    12601 accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc catcattctt
    12661 acctccgtct cttttcatct acccataaat aaataacata aataattcta aaaatattat
    12721 tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc cgccttaaaa
    12781 acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca aggtaacaaa
    12841 aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa cacagactga
    12901 aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata aaaaggaggt
    12961 atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg tcattcaaaa
    13021 aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac ggacaatcaa
    13081 agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg atgaatttat
    13141 ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta aaaagttagc
    13201 tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta actatttgat
    13261 gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg aacaacttta
    13321 tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct taataaaatt
    13381 ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg agttatctat
    13441 ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt ggccattttc
    13501 ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag aaaaaatttc
    13561 aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa acaaacaaca
    13621 ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat tgcagcatac
    13681 acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg aagagacaga
    13741 aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc aaatctcttt
    13801 tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta tttgtcgaca
    13861 agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt tagaaaataa
    13921 aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac tgttgggtga
    13981 atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc attctagttc
    14041 ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa aaaatgggga
    14101 aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac aaacagaaac
    14161 gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa attatcatct
    14221 tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg catattcaga
    14281 acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa cggagacttt
    14341 ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag cggttcatga
    14401 actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa attcttgtca
    14461 catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt agctcagatc
    14521 aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg aacaataaga
    14581 tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc agtgctgctg
    14641 caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg ccttcactca
    14701 aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc aattggaaaa
    14761 gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc cagctaagca
    14821 atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct gctggaacaa
    14881 gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca tgtccgcatt
    14941 catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct tgagacaaac
    15001 tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt tgttgaacac
    15061 cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta ccagtcacat
    15121 tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac gctgacgcaa
    15181 ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa aaaccataca
    15241 ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta tcattagtag
    15301 taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca aattccgatt
    15361 tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg ccttctacta
    15421 aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct tttactttca
    15481 agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt gcatctaata
    15541 ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg ttcccgcatt
    15601 ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat tctgggcaat
    15661 ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg taacggaaaa
    15721 agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt ttcgaacgaa
    15781 gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct ctttatagat
    15841 ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat ggcctcctcc
    15901 atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac gtacgcggta
    15961 ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa tcactccagg
    16021 aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa gaatttcttg
    16081 cggaaggatt attcttcctg caccattttc atccacctgg atattttgat aaagataacc
    16141 ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag cacttgcatc
    16201 aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg tggagggata
    16261 caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag ccttaacgtt
    16321 catctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac tacagctact
    16381 tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat gatggtcgat
    16441 tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg aattagctgt
    16501 atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt gcatttctgc
    16561 taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt ctgggaaact
    16621 ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat cagggcgcaa
    16681 atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt cttgctgtac
    16741 tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac gattgctttg
    16801 gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg tctgccgatt
    16861 tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg caaataatgt
    16921 aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt tttttgtgcg
    16981 acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc attgaccatt
    17041 tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc acggtcagta
    17101 tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat ctgcggttgt
    17161 cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc ttccaatgtc
    17221 aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg atggctaaca
    17281 gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac ccgatcgatg
    17341 atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat caaacgatga
    17401 tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg ctgataatca
    17461 gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata gatatcactg
    17521 acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat catggttgcg
    17581 actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc agctagcttt
    17641 gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc atcttccaat
    17701 gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg cgtaatatag
    17761 cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac cttgtctaat
    17821 cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc aaatgtcaaa
    17881 cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg ttgaattgtt
    17941 gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc ttcactgaca
    18001 ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa actttcaata
    18061 tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca gcgtttccga
    18121 gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt cgggatcttg
    18181 ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac gaattgtttg
    18241 cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg gcaagaaagc
    18301 gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag cagtatcccc
    18361 tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct cgcccatata
    18421 ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga attttaccac
    18481 gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta ctcctctgct
    18541 gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt cttttgcaat
    18601 aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc aatctgtctt
    18661 tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt caatcatcac
    18721 gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg taattttcat
    18781 attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca tcacttgcac
    18841 atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta cagtatgtac
    18901 aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat aatctcaagt
    18961 ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa atcagcgtaa
    19021 ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct cccgatttca
    19081 ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa aaaacaataa
    19141 tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata tcctgtatgc
    19201 attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac ttgtctcgtt
    19261 tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc agttttgttt
    19321 cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt ttgtagttct
    19381 tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct gatcttttgt
    19441 tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc tttattctat
    19501 cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg caaaaaaaga
    19561 gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt tttatttcga
    19621 cgttgagtag cttccataat gactggcaag atcactgggc ggcgttttgt ttgttcaaat
    19681 aagtaacggc ctaattgatc tcggatgtct tgctttaatt tgctccactc aaaatcattg
    19741 ctttctaaat gtttctcaac gattgtttca atcatattgc tgctttcttt catcagatca
    19801 cggctagctt ttacgtaaac gaatccccgt gaagtaattt gaggttttga aatgattttc
    19861 ttttctcgcc gactgatagt gacaaccgct acgaaaatcc cgtcttctga caagattttt
    19921 cgatcacgta aaacaatgtt tccgatatca cctatgccta atccatcaat cattacattt
    19981 tctgcacttg tagttcctgc aacactcata cgcttgttct tatattccag cacgtctccg
    20041 cgtccagtaa tatagatatt tttgtatggc atccctactg catgagccaa atctgcatga
    20101 gcagctaatt cacgatactc tccttgaact ggcacaaaat atgttggttt catcaaattg
    20161 atcattaatt gcaagtcatt aggattggca tggccagaaa cgcgcatatt ctcagagatc
    20221 aacttcactg ttccaccagc tcgataaatg atatcttccg ttttcgccac gatggtttcc
    20281 atcgcgatgc ttggcgtagt agtgatataa accaaatctc cttcttcaat cttgacatta
    20341 cggtgtgtac cgtttgccat tttttgcaga gacttgatgg gttcacccat tcggccagtt
    20401 tctaaaatga tcagttcttc tggatcatag tttttcatgt ctttcaacgt aacaagcaga
    20461 tcttcacttg gtaattgaag tttttctaaa cgcatcgctg tgcgaataat tcgttcgaaa
    20521 tcttgaccag tcaagacaac ttttcgttgt gctttttctg ctgcatccaa tacttgttgc
    20581 actcgttgca aattacttgc tacacaagcg acgatgatac gcccttccca atatttgatc
    20641 gtatcaaata cttcatctgc tatttgggct tcagaagcta caggctgagg attttctgca
    20701 tttgccgatt cacttagtaa ggctaagact ccttctttgc cgatttccgc caatcgtgca
    20761 taatctgttt gatacattgg gatagccgtt tggtcaaatt taaaatctcc cgtataaacg
    20821 atactacctt cttttgtttt cagattgatc cccactgaat ctggtatcgt atgagtggta
    20881 cggaaaaagc tcactactgt ttcaccaaag tctatttctg tatgctcatc taccacatgg
    20941 aaatccttaa atcctttggc tgcttcatta cgactgacat tcaattttgc taactcgatc
    21001 gtcaactctg tcccgaatac tggtacagaa atttttgaca acaagtaagg aagtgccccg
    21061 atcgcatctg catgtccatg tgttaaaaag acaccagcta cacgatcaat gttttcttct
    21121 aaatacgtaa aatcagggat tacgacgtcg atccccaaca actcattttc aggatatttt
    21181 agcccacaat ctaaaataaa aatatcctcg cccacttctg cgacatacat gttttttcca
    21241 ttttcacgaa cgccgcctaa tgggataatt ttaatggtac tcacaaactt cacctcttct
    21301 tatgacatcc aagtttaaag atgtcgattt tcttctttat ctaaatatat gagccattcc
    21361 aatgaagcat atgcaaaatc actatttctc aacttatgga gaatcagcag gaaaattacc
    21421 agcacatccg cgttctgaca gttggatggg cattttattt tggatatact tcattaacaa
    21481 aatggatctt ttgcgaaacg ctatagccag aatacttata taagtattca gctgttcccc
    21541 attattatac aacaaataaa ctaaatcgct agtatcagca attgttagaa caactatgca
    21601 cgtaaaaatc cctctgtagc atctaggatt tcttttgcta ttcctctttc atttcttctt
    21661 cgtcggtttg aaacatactt ttttcatttt accacatttt ttgcaataac tatagtgact
    21721 ttttatatat acagaacctc atgttgatgg tgtgcaaaca gcataagatt taagctagct
    21781 ttaagatagg ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa tatgaatttt
    21841 atgaaacgtg catggaaaag tacaaaagta aaatggggac gttcattgtt attgtttgcc
    21901 gtgttcacag ccattcttat ttttgtgcta gctggcctca cgattcgaag cgctgcagaa
    21961 gaagcagcga atcaagcaca aaaagaagtt ggagcaacag tgactttaag tgccaatcga
    22021 gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac tgaagaacgt
    22081 ccagatccag gcagtttttc tcttacacca gtttccttaa cggacgcgga aaaaatagct
    22141 gaattagata atgtaaagag ttattccttt gaaagttccg cctcagctat ggcaaaagat
    22201 ggaataactg ccatcactag ttctgattca agtgaagaca gttcagaaac tactgatgca
    22261 ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa tgactcaagc agactttcaa
    22321 atcagtggtg tgtcagcaac tgcacaaaat agtaattttt ctgatggaac agcaaaaatc
    22381 acggatggtg aaggaataga tgacagcgac aaaggcacaa acaatgtagt cattgattct
    22441 actctagcag aaacaaatga cctggcagtc ggagatactt tcacaatcac gagtactgaa
    22501 gatgaagata ccacttatga aatgacgatc aaaggaattt atgaatccag cgaaacaagc
    22561 ggcagtatgg gtatgaaatt caacttcatg aatccgtcga atacacttta cacttcttac
    22621 acttttgcaa atgaactaaa tgggacatct gacgataaca cgatagattc tgctatttac
    22681 actctatctg atccagataa aatggatgag tttctcaaag aagctgagaa gctgatcgac
    22741 acagatacat ttagcttaca atcaaatgat tccatgtatc agtcaatgtt ggaaccttta
    22801 aacaacgtgg caagtttttc taaaaatgtt gttttactag tggctgtagc cggtattatc
    22861 atacttactc tgatcattat gattaccatt cgggagcgtc ggcatgagtt aggcgtcttg
    22921 ctatctttag gagaatccag aagtaaaatc attctccaat tgtttaccga agtggctatt
    22981 tgtatgattc ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc gaatgccgtg
    23041 ggacagcagc tacttgacca acagacagaa accacgacac aaaaccagca agctcctggc
    23101 ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg tcaacaagga
    23161 accacgaata atccatttgc tgtttctgat caagtcagcg aattggaaat caatgtcaaa
    23221 cctgcacaat tgggactatt agccggagta gcatttggaa tcagtctgtt ttctgtcttt
    23281 ttagcttcta tagggatatt aagattaaac ccaagaaaaa tcctattaaa ctaaaggagg
    23341 aatttgatgt tacggacaga acaattggga ttttggtatc aagatccgca agatgcgctt
    23401 tataaagatg tagaactgaa tttcgaagct ggaaaaatgt acgctatttt aggagcaagt
    23461 ggttctggaa aaacgacttt tctttcgctt atttctggac tagatgcacc aaaagaagga
    23521 aaggtatact atcaagatcg ttcactgaac aaaatcgggt taagaagtta tcgtaaaaat
    23581 gatgtctcga ttattttcca agcttacaac ttgcttcctt atttatcggc tctagacaac
    23641 gtcatagcag caatggaaat cactaagacc aagcaagaga attatcgaca agttgcttta
    23701 gatcgactgg ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt gaccaaactt
    23761 tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta tctgctgtga acatcaacta
    23821 atcgtagcgg atgagcctac tggaaatcta gatgaaacaa cttcacaaga tatcgtcaaa
    23881 cttttccaag aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac tcatgagatt
    23941 gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt tcaaaaaatc
    24001 gaagtttaaa aaacaggata atgatcaaat aaaaaatccg aaccatatct gtctcgcaga
    24061 tatggttcgg attttttatt tgcttgattc aattagtaat aagtggtttt tatttttttt
    24121 cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa atttcgagct ttttctttga
    24181 tgtcagcacc tagtgattgt gctttccctt ctgtttcttt gctcttgtcg tctgttgctt
    24241 tgccgtatgc ttcttttcct tcaccgacta ctttatcttt catatcttta gaacgtcctt
    24301 ttaaatctgc cattgtgctt cctcctctta gtttgtttat tgatctacct tcagggtaac
    24361 atcaacaaca gatacagaca aacagaacgc tcgttttcta aaaaagatag ttagagctgt
    24421 tgattaaaaa cttttttcga aagtccccca tactttgact tctttttctt ggaccattat
    24481 ttctccttta gcgataactg tatcaatatc aagcgtttct ttatccaaga tacacagatc
    24541 tgcatcagca ccaatttcga tttttccttt tttatccaat ttcaatatac gtgctggatt
    24601 actggttata gctcgtaagg cgatttccaa agggatactt tctttttgca cagcttcttt
    24661 gatccctact agcaatgcct tggcacttcc tactcctaac cctaggaaat gattgttttc
    24721 atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat ctaaagaaac
    24781 atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga acttcaccat ctgtcttctc
    24841 ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt tggcaaatgc
    24901 cacgcaagct tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa attgtgtgat
    24961 cggaatttct gtttcttcta cgattcgtgt caatagctct agctttcttt ttcctccgcc
    25021 taagtggaca ttgatgatcc cagcttttcc ggataacatg cctcctacac gagcatctgc
    25081 tactgcacga gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg atactgcgat
    25141 ttctccgatt ccgataattt tatcaattgt caaaaagtct ttgatgatag aatctgttac
    25201 tgtttttacc ggcaatcggt acgaaccttc gtatacataa gtggagattc cttctgcttc
    25261 cagacctttc gctttgctga tcaacgcagt catatctcta ccttctccgt ctgttcccaa
    25321 acaaccgaca actgtcgtta caccagctgt agtcaattgg ctcaatgtga cttctggcgt
    25381 tcgattttga taaccgcctt ctcctcctcc acctaaaata tggaaatgac agtcaatgaa
    25441 acccggggtt agtacttttc cctcacctga aagttcttct acttccactc cttcgtagcc
    25501 gccttctagc tgttcttcta cagccaaaat cttcccaccg gcaatcagta catccatttt
    25561 tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg attttcatac atttttcctc
    25621 tttctttaag tataactgat gataggatct taaaaaataa tccaaacgat aggatcttta
    25681 ctcctttgat tttaccgtaa aatgacggtt cttggagtcc ttatctactg tttggattac
    25741 ttttttattt acaggtctcc agtactactg cagacctatt gcaatggcaa tcatgatgaa
    25801 aatagttcct aaaacaaaca ggaatccttg catcttgatt tggaatcttg cccattttgc
    25861 ccattcgatc cgtgcaacac ctagcacacc aatcaagctg gctgatacgg gtgtaaaagc
    25921 gtccataaaa cctgaaccaa gctgataagc aagaactgct acttgtctgg atacacctac
    25981 taaatctgcc agtggagcca tgattggcat agtcaaagct gcttgtcctg aatttgatgt
    26041 aacaaccaag ttgaacaaac tttggaagat gtacatcgcc caagcaccaa tgacagctgg
    26101 aacaccggtc aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg aaggaacaga
    26161 tgcatcagat cctcccaaga caagaagaat ccctttggcc atacctacta cgattgctgt
    26221 accagctaaa tcagctgcac cagattggaa agaagaagcg atgtcattga ctttcattcc
    26281 atctaatttg aaaagaacag caatgattcc agcaactaat cccatcacaa agaattgaga
    26341 agcgatttct ggaatgtaat agcctttttg agtcacgccc caaacgatcc agacaaggac
    26401 aactaacatc tctaataaga tcaatttatg cccaagcatg aattttttat tttcatcaga
    26461 tgtctttttg atatgagaac ggaaataatc atctgatttg taagtcaacg aactttctgg
    26521 ttttttacgg attttttctg catatatcat catataacca gcggctaatg cagtaacaac
    26581 tacccacata atcaatcgga atgtcgcacc agacaacacg gggattccag caatcccttg
    26641 agccaccgct acgctaaacg ggctcatcca agaggtcgca tttccgactt gagatgcgac
    26701 ataagtcacg gttactgcta cgatcgagtc atatcctaac gcaatgacaa atggaaccat
    26761 caccattgag aatggaatta cctcttctgc catcccgaat gtagcgccac cgaatgaaaa
    26821 agcaaagaat aatagcggta aagctaaacg ctccagtccc ttagttttac tgataaacgc
    26881 atagatacct gcgtcgatcg ctccggtacg cataataata ccaaaagcac ctccaacaac
    26941 aaggatcaat gcagcgatcc ctacagcaga tccatactta tctccagata ctagtccttc
    27001 aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct gttccccata ttttggctgt
    27061 tttatgcaat tttttagaag tatcatagat attttcgccg tactgatcgt aaagcacatc
    27121 atctgtcaag gagatttcat ctagtttttc ctgcgtaaga tttttttctc cgtcagcaat
    27181 gactttttcc aatccttttt ccggaacatc cagtttttca cgttctgctg gatgatcttg
    27241 cagttcttct aattgatcaa acacatacga cttatccagc tcgtaagcat aacggaagga
    27301 atcttgacgt aaaacagtac gagtacttgt ttcgccattt gcatctttgt attcaatatc
    27361 ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt atccatgcaa agatgactac
    27421 accaaaaata atgacatacg tatggggtgt cgataattta gtgaagtcgc gtttcggttt
    27481 tcctgaagaa tttgttttct tcaaatttat ttcttccttt cttttttcag taattttgat
    27541 gaataaaaaa tggataatca aatgaattat acagtaaaaa tgacatttcc acatgaataa
    27601 tttattagaa aatcact
//
LOCUS       DAZUMT010000026        27482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000026 DAZUMT010000000
VERSION     DAZUMT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.026"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..965)
                     /locus_tag="ACG8FM_001048"
     CDS             complement(21..965)
                     /locus_tag="ACG8FM_001048"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5093726.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1261..2205)
                     /locus_tag="ACG8FM_001049"
     CDS             complement(1261..2205)
                     /locus_tag="ACG8FM_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5093727.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2426..3640
                     /locus_tag="ACG8FM_001050"
     CDS             2426..3640
                     /locus_tag="ACG8FM_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5093728.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3662..5677
                     /locus_tag="ACG8FM_001051"
     CDS             3662..5677
                     /locus_tag="ACG8FM_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS5093729.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5760..7133)
                     /locus_tag="ACG8FM_001052"
     CDS             complement(5760..7133)
                     /locus_tag="ACG8FM_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5093730.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7159..7443)
                     /locus_tag="ACG8FM_001053"
     CDS             complement(7159..7443)
                     /locus_tag="ACG8FM_001053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5093731.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7445..9460)
                     /locus_tag="ACG8FM_001054"
     CDS             complement(7445..9460)
                     /locus_tag="ACG8FM_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5093732.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9639..9944)
                     /locus_tag="ACG8FM_001055"
     CDS             complement(9639..9944)
                     /locus_tag="ACG8FM_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS5093733.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10033..11064)
                     /locus_tag="ACG8FM_001056"
     CDS             complement(10033..11064)
                     /locus_tag="ACG8FM_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5093734.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11271..12146
                     /locus_tag="ACG8FM_001057"
     CDS             11271..12146
                     /locus_tag="ACG8FM_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5093735.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12298..13140)
                     /locus_tag="ACG8FM_001058"
     CDS             complement(12298..13140)
                     /locus_tag="ACG8FM_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5093736.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13232..15109)
                     /locus_tag="ACG8FM_001059"
     CDS             complement(13232..15109)
                     /locus_tag="ACG8FM_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5093737.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15106..16527)
                     /locus_tag="ACG8FM_001060"
     CDS             complement(15106..16527)
                     /locus_tag="ACG8FM_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5093738.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16530..19919)
                     /locus_tag="ACG8FM_001061"
     CDS             complement(16530..19919)
                     /locus_tag="ACG8FM_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5093739.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20176..21567
                     /locus_tag="ACG8FM_001062"
     CDS             20176..21567
                     /locus_tag="ACG8FM_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093740.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21640..22287)
                     /locus_tag="ACG8FM_001063"
     CDS             complement(21640..22287)
                     /locus_tag="ACG8FM_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5093741.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22295..22981)
                     /locus_tag="ACG8FM_001064"
     CDS             complement(22295..22981)
                     /locus_tag="ACG8FM_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093742.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22987..24060)
                     /locus_tag="ACG8FM_001065"
     CDS             complement(22987..24060)
                     /locus_tag="ACG8FM_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093743.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24282..24956
                     /locus_tag="ACG8FM_001066"
     CDS             24282..24956
                     /locus_tag="ACG8FM_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5093744.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24961..26301
                     /locus_tag="ACG8FM_001067"
     CDS             24961..26301
                     /locus_tag="ACG8FM_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5093745.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26450..27436)
                     /locus_tag="ACG8FM_001068"
     CDS             complement(26450..27436)
                     /locus_tag="ACG8FM_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS5093746.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7998 a         5209 c         5000 g         9275 t
ORIGIN      
        1 tagttcatat tcattgtttc ttataataca gtgttcaata cttctcttaa agcacttgtt
       61 gatttgctga acattgcttt ttcttcttcg ttgattgata attcaacaac ttcacgaaca
      121 ccattttcgt caacgattga tggcacacct gtaaagatat ctttttcgtc gtattcacca
      181 gttaaataag cagatacagg caatacagct tgttcgttat tcaagatagc ttttacgata
      241 cgtgcagtac tcataccaat accatagtaa gttgcttttt tacgatcaat gatctcataa
      301 gcagcattac ggactttatc agcaatcaca tctaattcat cttgggcaat acgatggtct
      361 ttttctacaa tttcaaacac tggtttacca ccgacagttg tatgtgacca tgcagcaact
      421 tctgaatcac catgttcacc taagatatag ccgtggacgc tgcgtgggtc aacttttaat
      481 ttcaatgcga tttctttacg gaaacgagtt gtatccaatg ttgttccagt accgataaca
      541 cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc tactgggttt
      601 gaagcaacaa cgataatacc gtcgaatcct gatcccatga tttcttttac gatttggcgc
      661 atgattgacg catttgtttt aactaaatct aaacgagatt gaccaggttt ttggttgata
      721 ccagctgtga taacaacgat atcagcatct ttacattctt catagccacc tgaccataca
      781 gccacatttt catcgcccca agccatacca tctaaaaggt ccaatgcttc cccttctgct
      841 ttttcttgat tcacatcgat caatactaat tcattagaga ttccttggtt gatcattgca
      901 taagcgatac ttgttccaac aaaacctgta ccgacgatta ctactttacg acttgttttt
      961 ttcatgaata aacagctcct tttcataact atttgttact ttcatgaaaa gtatagtctt
     1021 gtttattcat tttatcaaca tgttaattat aatagttgtt attataattt ttgatggaaa
     1081 cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata tcccctgata
     1141 atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca acgatataat
     1201 aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa gattttcttc
     1261 ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga ataatttgaa
     1321 taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg aaaaactcgt
     1381 ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt cttgaaagga
     1441 agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc caaaatgagc
     1501 tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta atactctaag
     1561 catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta ataaatgagt
     1621 caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat gactgtatgt
     1681 atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg tttttccgcc
     1741 gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta tcaaaaaaga
     1801 agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata aggtttcttc
     1861 ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc tttgagtagc
     1921 ttctttatca atgatcacca cgtcactttc ttccatctct atcctttctc cattaataat
     1981 ttgtaaacat gtaccgctat acatataatt gatttcaaca aaatcatgtg tatgctcggg
     2041 aacttccgca tatcgacggt gtttactgat atgaatattt ccctgttgaa atagatcccc
     2101 tcggtggatt tttggcggtg tctgaagatg gtaagctaag tttaaatcat tgatattcat
     2161 tccttgttca cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa atgtctaatt
     2221 cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac
     2281 gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg
     2341 acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat
     2401 aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct
     2461 gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat
     2521 gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc
     2581 ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa
     2641 acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc
     2701 acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat
     2761 caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat
     2821 gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg
     2881 acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat
     2941 tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg
     3001 cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt
     3061 tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac
     3121 gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc
     3181 aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct
     3241 tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct
     3301 gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta
     3361 cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat
     3421 cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc
     3481 ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt
     3541 gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag
     3601 agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa
     3661 aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg
     3721 ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg
     3781 gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc
     3841 ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca
     3901 tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg
     3961 acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg
     4021 ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga
     4081 tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga
     4141 aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga
     4201 actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt
     4261 agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa
     4321 agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca
     4381 acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc
     4441 ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt
     4501 gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc
     4561 attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc
     4621 attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt
     4681 gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat
     4741 cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg
     4801 gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga
     4861 aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg
     4921 cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca
     4981 agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg
     5041 cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt
     5101 cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt
     5161 attgaatgac actatcacac agattactac tccatatttc aaattctatg aacaagatgc
     5221 tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg
     5281 gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa
     5341 tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc
     5401 aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta
     5461 tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc
     5521 tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga
     5581 tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc
     5641 acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata
     5701 tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct
     5761 taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat
     5821 acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct
     5881 agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg
     5941 attttaagat ctcctaatac tggaaggata aataatggca gccaactaat caataagcta
     6001 ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca
     6061 gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag
     6121 cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat
     6181 acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt
     6241 acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc
     6301 ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt
     6361 acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca
     6421 agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag
     6481 ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt
     6541 ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg
     6601 tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt
     6661 ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact
     6721 aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat
     6781 ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa
     6841 gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact
     6901 actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg
     6961 attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg
     7021 atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg
     7081 aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt
     7141 tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt cttttacttc
     7201 ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt
     7261 atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac
     7321 atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt
     7381 gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt
     7441 catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta
     7501 ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa
     7561 gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat
     7621 tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc
     7681 catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat
     7741 actgctccac gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata
     7801 atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt
     7861 ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta
     7921 aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt
     7981 ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc
     8041 ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca
     8101 tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag
     8161 aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct
     8221 ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc
     8281 taatgccact tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg
     8341 cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc
     8401 tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag
     8461 catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac
     8521 gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa
     8581 attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa
     8641 aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa
     8701 atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa
     8761 tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt
     8821 ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg
     8881 cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat
     8941 aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca
     9001 tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc
     9061 taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta
     9121 tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa
     9181 aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt
     9241 gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg
     9301 ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt
     9361 gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt
     9421 ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact
     9481 tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga
     9541 ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga
     9601 ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt
     9661 tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt
     9721 tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa
     9781 gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct
     9841 tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt aggggccatt
     9901 gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt tcctcctagt
     9961 agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac aaacccgatt
    10021 cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc cttcttccaa
    10081 aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc cattaactaa
    10141 tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa ttactttttc
    10201 ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt cgggcaaatc
    10261 gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt cttgtcgatc
    10321 ttttgtttgt ttcacgaaca tccctttttt tccgtaaacg acgaaacttg gacgttcttt
    10381 gatgcggaaa taactggatt ttatcgaaat tttcatattt ccatagaaaa aatccagatc
    10441 gaagtaatca ttcattcgtc ctttccctaa aagttgacgt acatcatagc tgatatcatc
    10501 tggttcgccg aaataagaaa gaacttgatc tactgtatga caagcatgtc catataaata
    10561 gctgttcgca gtagaaaatt catgcgtatt ttctggtact tccggtctga aataatcata
    10621 atgcatttct acttctaaaa tatcccctaa ttttccgctt tcgattactt tttggactgt
    10681 caagaagtca gaatcgaacc gtctgttttg gtaacattgg ataaatagac ctttttcttt
    10741 tgctaatgca aataactctt ctgcttcctt tgacgtttct gcaaaaggtt tctctactag
    10801 tacattctta ccatgttcga gtgccatttt tccatattca taatgaaatt gactaggtgt
    10861 agtgatgaca actaattgga tctcaggatc atcccaaaga tcagacagct gatcagtata
    10921 ttcgatgtct tcgaatgttt tccacactgg ttcagatggg gaaaaaattt ttttgatatt
    10981 gattttgttc gtaagttttc ttgaaaaagg caaatgatag cgatttgtac tctttccatt
    11041 gccaagataa gcgattgtaa gcatgagtgg tgcctccttt tattgatgtt ttcagtatat
    11101 cctagaaaag cgcagaaagt atttataata gctattctgg taaaaaagtt cagaaaaaca
    11161 cttgttatca ttcggtttgg tgaaattaat gttcaaagta aggataaaaa tcaaaaagat
    11221 agtagcggaa ccaagtgttt atcagtaaga tagaatttgg aggtcagatc atgcttattg
    11281 aagaaaaact aacaaaacaa gagttgttta ccacgacaga gaaacggatt gtcgattaca
    11341 tccgtaggaa tatcgaagct gcagtctaca tgacgattga agaattagca aaagctacgt
    11401 atacttccca ttcagctatc attcgtttat gcaaaaaaat gggattcagc ggatttaaag
    11461 aattccgttt cgagctgggg cgggaagttc atcaattgat tgctcagttc aatcaaacga
    11521 ttgatcctaa ttttcctttt tcaagtgaag atagcactca gcaaattgca aagaaaatgg
    11581 cggatttatc gatccagtca ataaaaaaag cacaattgca gattgaacat gaagatttag
    11641 atcaaatcgg taaagtattg aacaaggcac agcgaatctt tctttttgca aaaggtgatt
    11701 ctcaaatcac ggcaaggaaa ttccagaata aaatggtcaa gttgaataag tttttgatca
    11761 tggctgaaga atacagtgat tcttcttgga atgcagccaa tttgactagc gaggattgcg
    11821 ccatttttat tagctacagc ggtcgcatcc atcattatga acgaataatg acctatttga
    11881 agcatgtaga ggcccctacc ttgctgatta ccggaaatca gcattcagaa atggccaaac
    11941 aggctgatat gtgtcttgtt atatcgcagg aggaatatga ttttgctaaa gtagcgactt
    12001 tctcctctca aattgccttc gattacgtat taaatacgtt attctcagtg atatattctc
    12061 aaaattttga agagaatatc cgcaacttga aaaacaaaca agaattgatg caaaaaggcc
    12121 ttcttaaaga atatcggaaa acttaagaaa agatcctcat ttagtaataa gaaagatagt
    12181 gtttgatagg ctctctttga tatcatcata aaaacaggct gaagttttct ttttcaaacc
    12241 tcagcctgtt tgtttacgga acgattcgaa tagatatatt atagaaatga ggaatagcta
    12301 acttctgttt cttattttga acgattgacg aaaaagatat ataagaagga ggaaaatcag
    12361 tatacatgca gctaacaatc catacaggaa aaatcgtttg ttctcggata ctttctgtac
    12421 ttctttttga acttcttttg catgataagg aacccggtgc cctctgacaa gtaagcgatg
    12481 actgttgacc atataaggtg tacaagttag gagtgtcacg aaatcctgcc ctttttcgat
    12541 tctcagatct tttgtatcag tcggttctac cgtcttgatc tgatcgactt gataagcgag
    12601 tgtccgtcca ttgatttcga tatagaaaga atcacctaaa gtcagtttcg gtaagtctgt
    12661 gaacaacttt gcttgaggca gtcctcgatg gctggaaata acggcatgtg tattttctcc
    12721 ccccgtagga taagacgttc cttccaataa tgaggcacct ttttctagca gcacagtatt
    12781 cgtttcatca aaaattggca aatggacatt gatctcaggg atcgtcaaaa taccgatcgt
    12841 atgtttctca aaataggttc tatccttttt cgcaggttct tttttcttag taaagggatc
    12901 tgcacctgga tttccacctt cttttgctaa ttgctggttt ttctttgtca tgttttcttg
    12961 gattttagcc atgacttctt cattttcttt gaccgcttgt tgctgataat gagagatgat
    13021 ctgctgatcc agataattat tcagcgcgtc gctaacaaat ggataagcta gtatccctat
    13081 tcctgagaga agcaagaaaa gcatcagcag atctagaagt ttccttttct ttggtttcat
    13141 ttgtgttctc cttgtgatga ctgtttgata ataaaagaaa aaggagcatt tgcgtaatcc
    13201 gctgctcctt tttccaattc ttgcaaaaag actaaatctg actgtgcttg cgtctagcaa
    13261 agtacagtcc agcaatcagt agaaggacta ctcctgcacc gatatacaca tagattccct
    13321 taccgcctgt tgaaggaagt gtacctttgt gtttatttgg tattttttca ggagaaatca
    13381 actgtcctgc tgttacataa gattgttcat cgatagtaaa tgctacacgg tttgttaatg
    13441 gaacatattt ttctggcgct ttcgtttctt ccagatagta cgtgccatat tttagacctg
    13501 tcacatcgat taaaccgtta cttgtggttg taaaaaccgt tgctgattct ttcgccgata
    13561 cccagctgac ggcttttgtg gatgggtcga tcgataaata tttcgctgtg tcactatctt
    13621 gatcacgaac gacgaattct gctccagcaa gtgtttggtc tgatgtaacg tcaccatcta
    13681 ctttaacaaa tcgtttgccc ccagtaacga catcgactga cggtggtgtt tgatcatttg
    13741 tatgcccgtt atcgacattt gcttggttag aaaatccttt ggttggatct gctttttcgt
    13801 tcaaatgcat atagtaaacg aatttcaatg taccgccagg agttaatgaa ggaatataat
    13861 tcggatcaac tgaaaccgtg aatccgtctg tttgctcagt gacagaataa tttactgggt
    13921 cgatttcttt atttccatca tataaggcat aagcatacgt tcctgaagaa tcattatcaa
    13981 atgttaaagc agcgtcatga gtatcgataa gtttgaatgt tgtgtacttg ttttgcgttc
    14041 cttctttatc agcgatacct aatgggatat tgacagaaat ctcatattga attttttcac
    14101 cgattgcgac atcttttcca ttcaattgag gtgttgtttt atcaacttta gacgtatcat
    14161 ttttgatggt cttttctact ggtgtttggc tatttgccag gatctcaaaa ggcgtgattg
    14221 tttgcttttc aatcatttcc gcattatcag gagcttttac ttcttctaaa ttatattttc
    14281 caacttctaa atgattaacg atcaactggc ctttttcaat agatgcctcg gcaaagtcat
    14341 tgttgccgat gtcataagaa tgaccagtaa tgaaatgttt ggctttggtt tgatcagttg
    14401 tccaagtgta caatccatct gtgacacttt ggatgtattt tttgacgctt ggtgttcctt
    14461 cttctttaga aataataaat tctgctccat ttagtgcttc gttttcggca gtaccgattt
    14521 ttgtaacttt caacgttcca tcattaccga ctgtattttt agggtagaga tggatagtat
    14581 ctagttcttc tgtcccgtat ttataagagc catctgcttg tttgatcatc tcatatacag
    14641 ggaaagctaa aaccatgttt gcggcagctg acactccgtc ttttggttct tctttgatcg
    14701 tgtagactgc atctttccca ttttgttttt taggtaaaga taaagtgaca tttccatcag
    14761 catctgtcgt tcctgaagca actggtgtac caggagtcaa agactggact gcttgttttg
    14821 ctgcatccac ggacgctcct ttatctcgtt gcgcataaaa ttcttgagtg acgttataaa
    14881 ctgaaaatga aatatcggct aatccttggt attgatcgaa ttcgctcatt tctttcccgc
    14941 tgttttggat taaaggatcg ggtaaatcag tcattttctt tttatgaagg atgacttgag
    15001 ctgctgtctc ctctgcaaac actttttttg cgccgaagct gtttgtgagt aatggtaaaa
    15061 taaggaaaag gactcctaac ataacgttta tttttttatg atttttcatt ttttcactcc
    15121 tgttcttttt ttgataaaca accagattgc aaagcttgcg aataccatac caacaacgcc
    15181 taaaagtgta tttttcgttt cgtttgtttt tggaagaaat ggacggctat tttctttttt
    15241 attgactact ttcaatatac tgtccgcctc tttatcctca gaaagaaaag ttgaaatggt
    15301 aaacggaatt tctgtttcac ttaaaatata accagaaggt gcttcgatct cttttaatcg
    15361 ataacttcct gttttcaacc cggaaattga aaaagcgcca tttttatcgg aaatcaggtg
    15421 aagcgcatct gattcgttct ttgtccactt gtatccgttg gctgtttgct ggaggtaatt
    15481 tcctgctttg tttttaataa ggaatactgc gccagctaat cgtttatctg gtttatttgt
    15541 ctcgattttg acaaattgct tactccctgt atatacagag actttctttg tactgacacc
    15601 aaaaccaaaa tccagtgttc cgttgttgtt gatttccttg ttcgcctgtg ctgtactgct
    15661 tagctgcatc tgataagaaa tggtcagttt ttttccagca tatttttcca acgaagggat
    15721 cgaaaataaa acatcaaatc cgtgaggttt cttgtgtatg acaaagcctt ctgtcagctt
    15781 ttctccattc gatgaaatcg ttaaactatt aggtaaaaac gttaatgctt catccgcact
    15841 atctgacaat tcgaactttt gatagtcaag gatatttaca ggaattgtcg tatgcaagga
    15901 ataagtgatt ttttcaccaa tcgtaaaatc atttctaggc tcttctaata ccttctcaaa
    15961 aggtggatca gggtagtcgt tttcttcatt tttaggatac aaatggatag ttgaaagttt
    16021 ttgattgttt tgtccatgta caggaagaac aactaccata ttctctgcct tttctttgac
    16081 gacttctggt actttggatt caatgaaaag atacgcttta tctctttttt cttgatcttt
    16141 agcggccaga gaaaaagaag cgataccatc ttcgttgtta agagttgtag ttgttgcttc
    16201 tgcggtaaac ataccggaag acgcaccgtt ttttgcgatc tctgcttgtg cttcttctac
    16261 cgttttgccc ttttcccgta gatggtaaaa agaatctgtg acatcataaa cttggaatgt
    16321 gacaccattt aatcctcgat acgtttgtaa taaagctttt tcttgtccgt catttggatg
    16381 atttttcggc agttgcccgt ttgggaaaag caatttgtgt aaagtaatct gaaccatttc
    16441 tgtttctgtt tctgttgcta cctgagtaaa agctggttta aatagtaaca agaagagaca
    16501 catactaagc caaccaagtt ttttcatatt tagctgcccc ctctttcttt tttcaaaaaa
    16561 agataccaaa tagaaaacgc acaaccaatc atccctacta gatagattcc taaacggcca
    16621 attcctcccg tttcaggtaa tggtactttt gcctgattcg tgatgtctaa agaaatctgg
    16681 ttgtttttgg ctcctggtga cagaacagat tcatgatctt gtccatccac ttgaattgac
    16741 ccatcttcgt gtataactat agtaactggc tcttttagac cttggtatcc ttctggtgta
    16801 aaagtttcgg tcagcgtata agttccaggt tttaaatttt cgaatagaaa ggtatctgtt
    16861 tcttgtccat cttttggcga ttctaagtcc atttctggtc cctgcagtct gaattttgct
    16921 ccttttaatg tctgtccgtt gtcttctttt ttgtgtaccg ttagatcaaa cgcttttagt
    16981 cgattttgat gagtgagcgt ccatacatta tttgtcatct cggtattttt tcccgtgtaa
    17041 atgatttttc catatttatc gatttggaat tcataatttc ccggcgaagt atcatatccg
    17101 gcaggaccag ctgtttcaac gactcgatat ttaccaggtg tgagtgaatc aaaactggca
    17161 agaccttttt cattcgttgt ttggctgtca attggctgat aagtaccatt tgcttctttt
    17221 ttctgtaggg aaaatgctgc tcctttaaga tttatctctt tgtccgtccc ttgcatcgca
    17281 tacttacgta ctgctacagg aacttcaaca aaaggatttt caatagtcaa ctggatcgta
    17341 tcatcggaag tagtgactgt ttttccatca atggttaccg taccatcaga agcgatcttg
    17401 atttcccaag tagtcttttt gcttaatcca tgcccttctg gagcttttgt ttctgtcagc
    17461 gtataggtca tttctttttg caatttgaca ttttctggaa gagaataggt tccgtcgcca
    17521 tgatctgtta gtaaagtatc aatagaatct cctgttaatt tgaaaacagc gccaataaga
    17581 tccttttcac ctgtagagga atttttcgtt attttcaagt ttaacggtgt gaattgttta
    17641 gtatttttcc atgtcattgc atctatttgt tgagaatcgt atcctggtac aggtaattct
    17701 ttgattgttt ggtaactgaa tgcttgacct tgattattgt attgaggtag tgataatatc
    17761 tcttgataac tttctccgct atttgcagat aatttgtcaa tgtctgcacg ttcccacgta
    17821 tttgttgtat ctttagctgg tttaatgatt cgaatatatc cgtttttcca agctgcagca
    17881 tttgtcgtat gttcccgttg aatctcaaaa gtaacttgat ctggacgatc agctagattg
    17941 ttgtcaaatt cttcccataa ctttttgatg tgaagactga ctccgggagc tttagcagat
    18001 ggtataccga attcagctaa ttcattggta tcaatgtttg gctggaaagt tgtcctgccg
    18061 ttcatttgat accagaaatt tggatggaag tcctcatttt ctgtttggat tctcacttgg
    18121 taatggattt ggatttcttg gtcttttcct aaatagatct gattgctctt gatggtattt
    18181 ccttctatgg aaatagatgg agtgaccgtt gtaggacttg tccccacact cttgactgaa
    18241 agtgtaccag gctgataaat gaatggttct gcgattggat catttatttg tccattgctt
    18301 acagttgctg agatctgtac agcttttttg gctaggtatt cagagatatc tgttgcatga
    18361 tcagctgatt catagtaaag atcgcctttt tcatctgatg aaaccatttg tcgcatacga
    18421 gactctactt ctgcttttga gagaccagct gccggatcgc tttgaagttg gatgccaaga
    18481 ccatgtattt cgattcctcg ttctttgagt gccatcgctt ctccgatggt tgcgataaac
    18541 gtactgtcga ttcttctgga tagattgttt tggtcaggtg catcatagat tcttgagatt
    18601 agagaagtat ttcccggccg atcttgcgta ttagaaaact gagttccgta ataattgctg
    18661 cttgattgtg cgtgtacccg ctgtacttta taggaaaatg ttggtacacc atccgtcagc
    18721 aaaacgatca cttttttatg tccatttgga acggatagca tgcttcctgc atctcttagt
    18781 gctttttgtg taaaagtacc accatttgtc cgtgaaggtg taatggattt tacttgattt
    18841 ttcactgaat caaatgaacc catctgtact gcaccgttac tgtagctata tccttcgctt
    18901 gagtagccga catatcccat attgattttg tctgtgatac cgctatctgc taaagtatcg
    18961 acaaaacgat cgacaccaat ctttacttca ccgatccgat tattgtcgtt catacttcct
    19021 gaccagtcta cgaccaatac gagatcaaga ggcgtgatat ctttttgtac atttccacga
    19081 gcattcaaat aaacatcaaa caatccgggt gtacttgttt cctttgcata ctttcgtatc
    19141 gcatagtctg cttgattacc ggttcctcca tattcgatat acgaatgggt ccgatcatta
    19201 ggatctccat tccaagaatt tataccatcc cattgatttg ttccgttttt attgccttga
    19261 tgatcaagaa catttgtatt tcctgtaggt tgccaactgg ctttaggata agtgccctga
    19321 tcatctgttg tatattctgt aggaatcgtt tgattagatg gcacactcac agctggcaag
    19381 gataatgtag gcaatgtaaa tgggctgaca gaattttcgt tttcttttga tgattcttct
    19441 tctatttcgt ctggctttgg tactgacaca tctgtatctt tcttattttc tgtctcatca
    19501 ataatgtcat attctttatt atcgacgatc agcttgtttg cctggctgtg gtaaagtgct
    19561 ggatcgattg gaaaactcag atcgatagtt tgtttagtgt ttgatgagat ttccaacgta
    19621 attttcccgc tatttacctg ataagcatag ccatctcctt cttttctttc cacatcactt
    19681 aagtaatttt cgactagacc aatttctttt tctatctttt cagcactggt gttattcgca
    19741 tttagcttga tgttcagtgc tttttttcca gactgatccg aaatgacttc agctgaaatt
    19801 gtttctaaaa ctgttggatc atctgtagtg tctgctattg cagataatgg cacaaagttt
    19861 gataaactca atattatcag catgaaaata actttcagtt tcttccctgt ggtcgtcatt
    19921 tttcccctcc tttttctttt tttctgtttt gacgtgaata gtgaaaatta ttaaaataaa
    19981 caggcgttac ataattttat ttattttaat atgataatta ttatacgaag ggcgctaaag
    20041 ggaatcacac caattttcat accaattggt ttcatttatg aagttgattc cttcgattaa
    20101 tgagcgtttc attgaaagaa aagggttgag ggagaaaaac atatgatcag gttgttggaa
    20161 ttaaactata gaaagatgga aaaaatcatt cgaattctgt ccatttctga caagtggatg
    20221 actaaaaaag aacttgccaa acgaattggt agttcagaat ctacatttgt aaggtatatc
    20281 gaagagatca aaatccgctg gggaacggat gtaacgattc aatcttcaca aaaattgggg
    20341 tatcgtcttg aacatttgaa tgtaccagct tatttaaatg ttctgatgga aatgtcgcaa
    20401 agttctacaa acagtcgttt attaaatgag attattgtca atccgggtaa aacgattgag
    20461 tattattgtg aaaaaatttc catcagccgg tcttctttta cacgtaagtt gaaacaatgc
    20521 aatcaaattt tagaaagcta tttattaaaa gtgatcgttg accaaggttt tcagttaact
    20581 agccttaaaa gtgagctcca gttgcgtatc tttgttacct ttttcttttt aagttactac
    20641 ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat tatggtcaag
    20701 aataattgtc agatgaattt gtttcaggaa gaaagcccat atgagcaatc tttctttatt
    20761 atgtattttg ttgtgcatct catgagagaa tcccaaggaa ctccatttga atggaaagct
    20821 ggagactctt atgtacagtt gataaatatc gataaaaaag acttcttaat cttgaaaaat
    20881 ctcttaggaa aattgaagta cagcagctat ataaaagtag tgaactattt tgtcaatacg
    20941 attttttcag aattttcgga gaaagaaaaa aatgaaatca gtgagcagat acgaaaaaat
    21001 atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt
    21061 caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac gactcatttg
    21121 acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg tctatatgct
    21181 ggatttcgtg cagaacttca attttattcc actctactga atcagaatct gatttattat
    21241 ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca ttatacttct
    21301 gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata tattgaagaa
    21361 tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc tattactgaa
    21421 gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa tcaacccatt
    21481 ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc aaatgaagaa
    21541 gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat aaaccaccct
    21601 gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc cttcagcaat
    21661 ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat tatttgcaat
    21721 gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac taactgcttc
    21781 ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt tttcgttcat
    21841 tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc gctgggttaa
    21901 caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc actctaaata
    21961 tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta agatatcttg
    22021 atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca aatcttgaaa
    22081 atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg agactttgac
    22141 ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc tctgtttctt
    22201 tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt tatctctgat
    22261 tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc tcttcttctg
    22321 tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata cggacatcat
    22381 gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt aatttgacga
    22441 cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag atcaagcttg
    22501 gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca gaaaggtctt
    22561 taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa tcgatttttg
    22621 ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc tttaccgtta
    22681 aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag cgtaaatctg
    22741 ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt tctccggaag
    22801 tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa ccagaaggac
    22861 caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc aaagctgaga
    22921 cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat atttttttca
    22981 tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa
    23041 caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc attgtgacca
    23101 aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag caaacaaaag
    23161 agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag atttagcgat
    23221 gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct tctgaagtgt
    23281 catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa agtatatcat
    23341 cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga atgtcccaat
    23401 agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg aatcctttat
    23461 tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc catacttcaa
    23521 agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat aagtttcagg
    23581 aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg tatctccaat
    23641 tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg ttttaaattc
    23701 atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga aaactgtaac
    23761 attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg ctttctctcc
    23821 tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata cttgatttgc
    23881 atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt taaactcttc
    23941 tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca agaacataat
    24001 accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag ctagaaacat
    24061 ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg aatagtgtaa
    24121 ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat tgtctaagac
    24181 atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt atttatgctt
    24241 tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa ctattagtag
    24301 tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca atcggcgaga
    24361 taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc
    24421 ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta gcaaaaatac
    24481 ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt ttggaagata
    24541 agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc tatcgagaag
    24601 aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt gatgaaaatg
    24661 ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg tataaagaac
    24721 aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta caaaataagg
    24781 gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat tctgatacga
    24841 cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga agtactggat
    24901 tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg gtgtgatgca
    24961 atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca
    25021 tttgctttta tcttttttct tttgggattc attactttac agatattaaa ccaatctgca
    25081 tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac agtacaatta
    25141 gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac ggaacaaaac
    25201 agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa cactcaagtg
    25261 atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg gcgtttcaac
    25321 caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa tattgaatta
    25381 aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa agcggagacg
    25441 aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat caagaattca
    25501 ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat tctctctatt
    25561 ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc ttggagaaga
    25621 caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac gattattcaa
    25681 aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga atccgaaagc
    25741 atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga tttgctgaca
    25801 atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa
    25861 tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg aaaagagttt
    25921 attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat ccatcaagtt
    25981 ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa gatcattttg
    26041 gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg cccttctatc
    26101 agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca atctcgggcg
    26161 aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat cgaacagcat
    26221 catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa agtccaattg
    26281 ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc
    26341 acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg gaaataagcc
    26401 gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct tacgattttt
    26461 tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct tctcggctaa
    26521 catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca acttcttcac
    26581 gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata gtaatcgtgt
    26641 catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt ttgcgttcga
    26701 tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg accacatgtt
    26761 tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat ttttcttctt
    26821 ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca gtcttgcttg
    26881 tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt gtcatattca
    26941 atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga tctgttccag
    27001 tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct tgtgtatcca
    27061 ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca aattttccat
    27121 cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc gtctcagaat
    27181 cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg tcttcttcac
    27241 catccatccg attttcatct gtttcaacat ctataccaga caatccgtca aatcctagtc
    27301 tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac ccttcgttac
    27361 gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg atgaccgtta
    27421 aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg tatattgtta
    27481 ct
//
LOCUS       DAZUMT010000027        26974 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000027 DAZUMT010000000
VERSION     DAZUMT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.027"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..1145
                     /locus_tag="ACG8FM_001069"
     CDS             141..1145
                     /locus_tag="ACG8FM_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093747.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1146..2090
                     /locus_tag="ACG8FM_001070"
     CDS             1146..2090
                     /locus_tag="ACG8FM_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093748.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2090..3052
                     /gene="opp4B"
                     /locus_tag="ACG8FM_001071"
     CDS             2090..3052
                     /gene="opp4B"
                     /locus_tag="ACG8FM_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5093749.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3079..3993
                     /locus_tag="ACG8FM_001072"
     CDS             3079..3993
                     /locus_tag="ACG8FM_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093750.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4019..5800
                     /locus_tag="ACG8FM_001073"
     CDS             4019..5800
                     /locus_tag="ACG8FM_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5093751.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6148..6834
                     /gene="rnc"
                     /locus_tag="ACG8FM_001074"
     CDS             6148..6834
                     /gene="rnc"
                     /locus_tag="ACG8FM_001074"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS5093752.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6854..10435
                     /gene="smc"
                     /locus_tag="ACG8FM_001075"
     CDS             6854..10435
                     /gene="smc"
                     /locus_tag="ACG8FM_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS5093753.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10444..11253
                     /locus_tag="ACG8FM_001076"
     CDS             10444..11253
                     /locus_tag="ACG8FM_001076"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5093754.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11264..12262
                     /gene="ftsY"
                     /locus_tag="ACG8FM_001077"
     CDS             11264..12262
                     /gene="ftsY"
                     /locus_tag="ACG8FM_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS5093755.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12449..14716
                     /gene="gshAB"
                     /locus_tag="ACG8FM_001078"
     CDS             12449..14716
                     /gene="gshAB"
                     /locus_tag="ACG8FM_001078"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS5093756.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14805..15257)
                     /locus_tag="ACG8FM_001079"
     CDS             complement(14805..15257)
                     /locus_tag="ACG8FM_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS5093757.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15407..16357
                     /locus_tag="ACG8FM_001080"
     CDS             15407..16357
                     /locus_tag="ACG8FM_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5093758.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16350..17309
                     /locus_tag="ACG8FM_001081"
     CDS             16350..17309
                     /locus_tag="ACG8FM_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5093759.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17306..18061
                     /locus_tag="ACG8FM_001082"
     CDS             17306..18061
                     /locus_tag="ACG8FM_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093760.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18084..19037
                     /locus_tag="ACG8FM_001083"
     CDS             18084..19037
                     /locus_tag="ACG8FM_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5093761.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19681..19977)
                     /locus_tag="ACG8FM_001084"
     CDS             complement(19681..19977)
                     /locus_tag="ACG8FM_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS5093762.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20332..20688
                     /locus_tag="ACG8FM_001085"
     CDS             20332..20688
                     /locus_tag="ACG8FM_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5093763.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20698..21597
                     /locus_tag="ACG8FM_001086"
     CDS             20698..21597
                     /locus_tag="ACG8FM_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5093764.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22117..22845
                     /locus_tag="ACG8FM_001087"
     CDS             22117..22845
                     /locus_tag="ACG8FM_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5093765.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22990..25017
                     /locus_tag="ACG8FM_001088"
     CDS             22990..25017
                     /locus_tag="ACG8FM_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS5093766.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25125..26222
                     /locus_tag="ACG8FM_001089"
     CDS             25125..26222
                     /locus_tag="ACG8FM_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5093767.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26529..>26974
                     /locus_tag="ACG8FM_001090"
     CDS             26529..>26974
                     /locus_tag="ACG8FM_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093768.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         9047 a         4668 c         5690 g         7569 t
ORIGIN      
        1 gtaaagcgat tttctatata attattaata attcattaca caaagataag aataaccttt
       61 gttataaaac gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg
      121 aagcagagag gggtattcga ttgtccgata accaattatt agaagttcag aacctacata
      181 caggattccg actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag
      241 acaaaaacga gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta
      301 ctacgattat gggattactt gatcctaata acacaaaaat tacaggtgag attttatata
      361 acgatctgaa tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata
      421 tcggaatgat tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc
      481 agatcaaaga aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg
      541 cactagaatt gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc
      601 cccatgaact atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca
      661 aacctccaat cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac
      721 agattcttga tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca
      781 ctcatgactt aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac
      841 agttcgttga agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac
      901 gctctttact gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg
      961 aaggtgtcgt tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta
     1021 ttccatggat accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga
     1081 accacttggt acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg
     1141 tgtagatgac agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg
     1201 gattcttcaa tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg
     1261 aaaaaggaaa gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa
     1321 aagccgttgt gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg
     1381 taacgaagcg aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc
     1441 aagattcaat gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca
     1501 tccgaaattt tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag
     1561 atatcgttgg gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac
     1621 aacgtcaacg tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg
     1681 atgaacctgt atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac
     1741 gaatccagca agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa
     1801 aacacatgtg cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa
     1861 gagaagacat ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc
     1921 cgcaaatcga tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag
     1981 agtatatcca aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta
     2041 cgatcacaac tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac
     2101 gattctgcga cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt
     2161 cttattagcc caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga
     2221 tccggcagtg atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca
     2281 gtacttccgt tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa
     2341 acgctctgtt gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt
     2401 gacagttgtc cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca
     2461 aaattcaatc ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt
     2521 atttatcttc ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac
     2581 aagtggatca caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt
     2641 gaaatatatt ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca
     2701 atatctgcga agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc
     2761 caaaggggta ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc
     2821 gatggcttct caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga
     2881 aaagattttt ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga
     2941 ctataccgtg atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt
     3001 actttcagat atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc
     3061 agaaaggaga atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct
     3121 cctatgggga tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca
     3181 ttaggtttgc tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa
     3241 gacaaagtga tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca
     3301 caaggacaaa agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg
     3361 atcataggtg ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc
     3421 atcggggttg gattagggat catctctggc tactacggag ggatcattga caacatcttg
     3481 atgcggatcg ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta
     3541 tcgattattt ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg
     3601 gtagctaaag cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc
     3661 agtgcctcaa aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg
     3721 aatctaagtt cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc
     3781 gagacaactt tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca
     3841 ttgatcggtt atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc
     3901 gcatcaatcc ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag
     3961 cgttctgcag atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat
     4021 gaagaaaacg atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg
     4081 gggcggaggt acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga
     4141 attaactttt ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga
     4201 agctgcggtc gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga
     4261 ttcatatgat gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga
     4321 tttcagaatc acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc
     4381 gacaatcaaa gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga
     4441 tgtgattttc ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga
     4501 cactttccgt aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc
     4561 cggaatcaaa aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag
     4621 catgcttcaa tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg
     4681 tgtagcaatc aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg
     4741 cccttattac atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca
     4801 ctactggaat ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag
     4861 tgcaactgaa gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac
     4921 ttacgatacc tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta
     4981 tacttatcta ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa
     5041 tccagattca aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa
     5101 taatgcagta ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc
     5161 tccagtattc cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa
     5221 agcgaaaaaa ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga
     5281 agacaaagat ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc
     5341 gcaaccattg gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata
     5401 cacaacaggt cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc
     5461 tgaaattgat gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact
     5521 atggggacca aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt
     5581 gatcaacgat atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca
     5641 aaaatggcaa caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga
     5701 agtattgcca gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc
     5761 atgggcaaat gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa
     5821 agggcgatgt cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt
     5881 caaggcgctg atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat
     5941 taagtcaaat ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc
     6001 gaaactaaac gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt
     6061 accttttgac cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa
     6121 aaaatatcca taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa
     6181 aactataaca tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc
     6241 tatgtgaatg agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga
     6301 gatgccgttt tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca
     6361 gaaggaaaat tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt
     6421 gccaaagaat gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga
     6481 ggacgtacgc gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat
     6541 cttgaccagg gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc
     6601 aaagcaggtg ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa
     6661 aaatcaggtg atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa
     6721 cgagtatttt ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa
     6781 tcgaaaaaat tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa
     6841 gtgagggaaa gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga
     6901 caagacagtc attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg
     6961 aaaaagcaat attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct
     7021 gcgaggcgga aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa
     7081 tattgcagaa gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa
     7141 tgagatcagt gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa
     7201 acaaagctgc agattaaaag atatccaaga attgttctta gattctggat taggaaaaga
     7261 atcgttctca atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga
     7321 ccgtcgtggg atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa
     7381 agcagaacag aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca
     7441 cgaattggaa gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt
     7501 acggctaaaa gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac
     7561 agcaaaaaaa gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg
     7621 caaactatct gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa
     7681 tgcgcaagcc gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa
     7741 actgaagcag acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa
     7801 aagctcgcaa gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga
     7861 aaagcttcaa gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc
     7921 tgaagcaaat ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga
     7981 attattggca gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg
     8041 aaacgaactg aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac
     8101 gttagccaag caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga
     8161 attgacacaa aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga
     8221 aatgatccaa caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat
     8281 gtatcaatta atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat
     8341 ccaagaaaac tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca
     8401 gctttcgggt attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt
     8461 agctatcgaa gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga
     8521 tgctcggcaa gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc
     8581 actgacgaca atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt
     8641 agaagggttt atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt
     8701 ggttcaaaac ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat
     8761 cgcgcaaacg atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc
     8821 cggaggatcc atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca
     8881 aaatcaagaa ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc
     8941 acaagaaaaa aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga
     9001 agttttgcgt acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct
     9061 acaaaacatc ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga
     9121 gacacgtgaa ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga
     9181 caaacaaaca gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag
     9241 tcaagaaagc gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga
     9301 acaagcagat cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg
     9361 tgcccgagtc caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc
     9421 gacattgaca gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat
     9481 taacgaatta tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca
     9541 gcgagataga aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg
     9601 tgagcaaaaa gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga
     9661 aatgatttta gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa
     9721 agcttcccaa gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc
     9781 cttaaaagaa cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga
     9841 acaagtcagt gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa
     9901 aaaccaattg tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga
     9961 agtatttgaa gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg
    10021 taaagctgaa ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga
    10081 agtacaacct cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc
    10141 acttacagcc atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat
    10201 cttagatgag gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt
    10261 gagcgatttc caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt
    10321 cgcagcagat gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc
    10381 tgtacggatg gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg
    10441 aaaatgatca aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg
    10501 atttcagatg agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt
    10561 ctatgtacag gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta
    10621 gatgaaggtg actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt
    10681 gaaatcatgg aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct
    10741 cagcaattgg atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt
    10801 gccccaagga agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg
    10861 gatataagcg atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa
    10921 gaagaattag atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc
    10981 atcaaaacaa gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt
    11041 atttctttat tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat
    11101 gaggaaaatg atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct
    11161 gtgcctttcg tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta
    11221 gccaaagtga tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa
    11281 aatcaagcgt gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta
    11341 cgacaagggt ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc
    11401 caattttcga acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc
    11461 agatgtaggt tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact
    11521 cagaaatgcg aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct
    11581 ctatgaagcg gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac
    11641 agttattttg tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc
    11701 ccatcagttc aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc
    11761 tggagcaatc gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg
    11821 caatgcagga ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga
    11881 gcaggcagat gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat
    11941 gaacgaatta gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga
    12001 aacacttctt gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt
    12061 caaagaaaca acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg
    12121 cggtatcgta cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg
    12181 tgaaggaatc gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa
    12241 aggattacta aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca
    12301 gctccttgtt tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata
    12361 cttatttgta agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat
    12421 tagattcata tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt
    12481 caatgcgcgc ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt
    12541 tgatcttgca ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta
    12601 tcacccctat atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac
    12661 cgattctatt cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc
    12721 tataccgaaa gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga
    12781 cgaagagatc attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt
    12841 agcaaaaaaa tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt
    12901 tggcgatgag ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga
    12961 attcaaaaca gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat
    13021 cacttattta ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca
    13081 tccgcaagaa cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa
    13141 tgtgaaagtt tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga
    13201 agaagggaaa ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa
    13261 gaaagtcgca gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt
    13321 gaatccttat gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct
    13381 gatgtacatg ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac
    13441 gactcgaaat aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga
    13501 aggtagagaa attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc
    13561 tctttattta gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc
    13621 gaaactctat caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca
    13681 tcaatattat aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga
    13741 actttctact cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt
    13801 agatgagtcg gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa
    13861 tgcaaacatg acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt
    13921 aacgaaaaaa gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc
    13981 catggaagaa gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc
    14041 taaatcaacc aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga
    14101 ttatcaagca ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt
    14161 tatgccggga acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt
    14221 gcgagtccct gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga
    14281 aaaaaatagt gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt
    14341 aggtgaatta gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca
    14401 agccaatcaa attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat
    14461 cgatatgact gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc
    14521 actaggagct aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac
    14581 aactgataaa aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca
    14641 tgtttatcct tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata
    14701 tcctgaagta ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga
    14761 tcaaaagctt tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc
    14821 cagaagcttt ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg
    14881 tatttttttt cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat
    14941 ccgaattcct caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt
    15001 ttcgttgcta gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt
    15061 atggaagcat ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg
    15121 atcatttcag caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta
    15181 tgctgttctt cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt
    15241 acatcatctt gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata
    15301 gcatattccc aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca
    15361 cttaaaataa gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg
    15421 actagtagca ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt
    15481 acaattgtca gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg
    15541 tttgccacga acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat
    15601 catgcaacat ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag
    15661 tgcgcgttta ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg
    15721 atccttgaca gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg
    15781 attgttcctc cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat
    15841 cattggttca gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc
    15901 atggcttcaa gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt
    15961 tgttccattg ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg
    16021 cgaatcgttt gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg
    16081 cttagtatca ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg
    16141 agtgatcatt ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg
    16201 cgtaactgct ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt
    16261 gatttttcct tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt
    16321 cctttatctt ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg
    16381 gtatgcttgt tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa
    16441 actggcattt tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc
    16501 tagctgtcag cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc
    16561 cttcagtatt gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta
    16621 tcggaggagt gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc
    16681 tgatggtcgg gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc
    16741 tttttttatt actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct
    16801 ttttacaagt catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa
    16861 gctttggcaa tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct
    16921 ggtacctgtt atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag
    16981 cagtaaacct cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc
    17041 ttttgacagg gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg
    17101 caaatatcag ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct
    17161 ttctggtagc tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct
    17221 ggaatacaac agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat
    17281 tgattcgcga aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca
    17341 tggaaaatca gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt
    17401 tatcggtcca aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc
    17461 aaaagataca ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga
    17521 attagccaaa aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt
    17581 gaaagaatta gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga
    17641 cgaggaaatc gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc
    17701 gattgacact ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca
    17761 agacacggat tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt
    17821 tcaaatcatg cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt
    17881 tcttcatgat atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg
    17941 acagttattt acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt
    18001 atacgaaatg gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta
    18061 aaactaacat taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat
    18121 catttggttt agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa
    18181 gcagcacaca aactgaaact acattgacga taacagatag taatggagac caaatcgagg
    18241 tgccaaataa tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg
    18301 ctcttggtgt tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt
    18361 catcctataa aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta
    18421 atcagcttca gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc
    18481 taagtcaaat tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt
    18541 tccagcaaaa tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc
    18601 aattagagga attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca
    18661 aaaaagcact agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta
    18721 gattcggttt catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat
    18781 ccactcacgg tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat
    18841 ttgtagtaga cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca
    18901 atgaactggt agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag
    18961 atgtctggta tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga
    19021 atcaagcatt taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa
    19081 tgtttcttac attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc
    19141 gtaaaatcgt attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa
    19201 cgtgaattca ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag
    19261 tgtgatccat tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc
    19321 gaaacttaga taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac
    19381 gatgttccat ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta
    19441 ttttctgtcc agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa
    19501 cgatgttcca tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct
    19561 attttctgtc cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa
    19621 acaatgttca atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat
    19681 ttaggcatgt gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc
    19741 ggcgggaagc ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat
    19801 tttatactcg cttaatatca atccaaaact attcgttttt tttgaagcta attttttata
    19861 aatatccaat gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga
    19921 accctcgata ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt
    19981 gattcctcca aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat
    20041 ccgtgaacaa aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt
    20101 attttttgtt atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct
    20161 ttttccgttc aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa
    20221 atcataataa acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat
    20281 tagttgcatc tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta
    20341 gaagtgacaa aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt
    20401 ttattagata tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg
    20461 gatacggcat ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat
    20521 tctgtagtgg ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac
    20581 atggatgatc catccacact tgctttagaa ccaacttata atagtttaca attaaaagga
    20641 ccaagcggct tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg
    20701 ggaaaattgg cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag
    20761 ctgatgcagc tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga
    20821 gatacggcta ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga
    20881 ttatctacga catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg
    20941 atggaccatt ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag
    21001 accgttcttt tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac
    21061 gaggcaatcg tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa
    21121 acagatctcg aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg
    21181 gaagcaaaaa aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc
    21241 atgtatcaat ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa
    21301 tcattcggag aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg
    21361 catcgtcgct ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt
    21421 tattattatg aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc
    21481 tatgatccta tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat
    21541 caaacacaac atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac
    21601 agcattagta atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta
    21661 gatccattta tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt
    21721 gatcggatga tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg
    21781 ataatcacaa aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga
    21841 agtctaggca gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa
    21901 ggctatgaca gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat
    21961 caaatcgggc tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg
    22021 ttcttttatt tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg
    22081 atatgtttag taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca
    22141 gcagcaatga tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta
    22201 tacccaaagg aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta
    22261 actcctcctg ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct
    22321 ccaagccctg gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg
    22381 actcaaaaaa tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat
    22441 gggtctagaa aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg
    22501 aaattaagtg tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga
    22561 gcacagctga aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg
    22621 caaacggtga attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat
    22681 gctaaatcag gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa
    22741 aatcaagatg cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat
    22801 tccactaaat tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac
    22861 tgttaaaaat aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa
    22921 ggctaggcta aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga
    22981 aaagatatat tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat
    23041 atctttccga tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga
    23101 gacgaagaaa gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa
    23161 gaaagcagtg aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct
    23221 gagggttcgg acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac
    23281 gatcccaata ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc
    23341 atattggaaa atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct
    23401 atttggtcaa ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac
    23461 ttaggaaatc aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct
    23521 agaatggcta gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat
    23581 tctactcgtt ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat
    23641 aaaaatagtg gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat
    23701 ggacatgtcg cttttgtcac gcctaaacca aataataata attattctgg agagcatact
    23761 ggcgtgacgg tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa
    23821 tgggacgcgg agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg
    23881 attatacctg atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct
    23941 tcaacaggta ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc
    24001 acatcaacag ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga
    24061 ggaaacgata agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat
    24121 aagaaaatgc agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg
    24181 atcaatggaa atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta
    24241 aatctgacag attattttat tgctggagat acgttaataa tcgaattgga gacaactttg
    24301 caggaagtaa ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat
    24361 ttgattgagc aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc
    24421 catgtttatt ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact
    24481 gggaaaatag gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac
    24541 tcggattcag cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat
    24601 tacagaaaag gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat
    24661 aaaatatcca gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata
    24721 gatgaacggg cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat
    24781 ggagattttg agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata
    24841 gggacatccg ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct
    24901 cttttagatt ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga
    24961 atcggaacct ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag
    25021 gaagagaaga atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct
    25081 tgtgtcccac agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga
    25141 ttttcttgag accaaaacaa caattattta taacactcct agttatttta ggggcacttt
    25201 ttaacaatat atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag
    25261 agaatcaaat cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag
    25321 tagaagtaaa agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg
    25381 tgaatagcgc aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa
    25441 aagacatgag cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc
    25501 taaaacctaa aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg
    25561 atggtgtatt ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag
    25621 aaactaagga tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga
    25681 tgcaacagac actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc
    25741 aaatcaatgc aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca
    25801 atcaagtagt tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag
    25861 aggaaaaaga aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa
    25921 atggtcaacc gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa
    25981 atgaaaatgg aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat
    26041 gggtatttga aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg
    26101 atgttacgat taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat
    26161 tgtttatttt attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt
    26221 aaggttaaga taacaaatag taaattttta ttattgtagg atggttgggc tctttttata
    26281 acaatataaa atataccatt ttattaataa aaatgcatac tagtaaatga tataattcta
    26341 ataacaggtt tgatcgcgag aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag
    26401 acaggacgat gtatattata caaacaagaa tcgctgacaa cagtgactcg gatccaggaa
    26461 atcagtttta tccccagcta gttggtgaat gcatggaaag taatatacgt agaaagagct
    26521 gatgactaat gaattatgag ttttttttaa gcaaagaaga tcacagaagt tattcattac
    26581 ttaaatattt agaagcaagc tcaaatttat cagagtcaat atcgaatgtt caagaagagt
    26641 tgtcgctatc gacatttctc ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa
    26701 agttcgatct agagaaaaat ttgcgagtaa ccgtcagtga attggatata tgtctggaaa
    26761 ttaatggaaa atgttctagc aaagctctgt tatctcgtta tataacggat tctttatcta
    26821 tgaaaatggt attggctttc tttcaggaaa aatatatctc agtagaaaat tttgctaaac
    26881 agcatcacgt gagttattct gtggcttata aagtattaca aagactaaaa agaaatctaa
    26941 aaaaatatca aatttttttt gaaaagagaa agtt
//
LOCUS       DAZUMT010000028        26160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000028 DAZUMT010000000
VERSION     DAZUMT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.028"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACG8FM_001091"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACG8FM_001091"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS5093769.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="ACG8FM_001092"
     CDS             1286..2461
                     /locus_tag="ACG8FM_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5093770.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="ACG8FM_001093"
     CDS             2465..3106
                     /locus_tag="ACG8FM_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093771.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="ACG8FM_001094"
     CDS             3103..4020
                     /locus_tag="ACG8FM_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5093772.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="ACG8FM_001095"
     CDS             4017..4688
                     /locus_tag="ACG8FM_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093773.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="ACG8FM_001096"
     CDS             complement(5229..5717)
                     /locus_tag="ACG8FM_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS5093774.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="ACG8FM_001097"
     CDS             complement(5918..6184)
                     /locus_tag="ACG8FM_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5093775.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="ACG8FM_001098"
     CDS             complement(6184..6447)
                     /locus_tag="ACG8FM_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5093776.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6968..7108
                     /locus_tag="ACG8FM_001099"
                     /pseudo
     CDS             6968..7108
                     /locus_tag="ACG8FM_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7250..9073)
                     /locus_tag="ACG8FM_001100"
     CDS             complement(7250..9073)
                     /locus_tag="ACG8FM_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5093777.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="ACG8FM_001101"
     CDS             complement(9671..12376)
                     /locus_tag="ACG8FM_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS5093778.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="ACG8FM_001102"
     CDS             complement(12682..13629)
                     /locus_tag="ACG8FM_001102"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS5093779.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="ACG8FM_001103"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="ACG8FM_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5093780.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="ACG8FM_001104"
     CDS             complement(14264..14626)
                     /locus_tag="ACG8FM_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5093781.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACG8FM_001105"
     CDS             complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACG8FM_001105"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS5093782.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACG8FM_001106"
     CDS             complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACG8FM_001106"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS5093783.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="ACG8FM_001107"
     CDS             complement(16254..17765)
                     /locus_tag="ACG8FM_001107"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5093784.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACG8FM_001108"
     CDS             complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACG8FM_001108"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS5093785.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="ACG8FM_001109"
     CDS             complement(19592..20668)
                     /locus_tag="ACG8FM_001109"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS5093786.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="ACG8FM_001110"
     CDS             20864..22609
                     /locus_tag="ACG8FM_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS5093787.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="ACG8FM_001111"
     CDS             complement(22889..23326)
                     /locus_tag="ACG8FM_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS5093788.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="ACG8FM_001112"
     CDS             complement(23310..24683)
                     /locus_tag="ACG8FM_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093789.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="ACG8FM_001113"
     CDS             complement(25005..25493)
                     /locus_tag="ACG8FM_001113"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5093790.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="ACG8FM_001114"
     CDS             complement(25515..26117)
                     /locus_tag="ACG8FM_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5093791.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7504 a         5561 c         4837 g         8258 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat caggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DAZUMT010000029        25842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000029 DAZUMT010000000
VERSION     DAZUMT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.029"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..781)
                     /locus_tag="ACG8FM_001115"
     CDS             complement(62..781)
                     /locus_tag="ACG8FM_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5093792.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(912..2063)
                     /locus_tag="ACG8FM_001116"
     CDS             complement(912..2063)
                     /locus_tag="ACG8FM_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS5093793.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2280..3020)
                     /locus_tag="ACG8FM_001117"
     CDS             complement(2280..3020)
                     /locus_tag="ACG8FM_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5093794.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3024..3347)
                     /gene="rsfS"
                     /locus_tag="ACG8FM_001118"
     CDS             complement(3024..3347)
                     /gene="rsfS"
                     /locus_tag="ACG8FM_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS5093795.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3404..3997)
                     /gene="yqeK"
                     /locus_tag="ACG8FM_001119"
     CDS             complement(3404..3997)
                     /gene="yqeK"
                     /locus_tag="ACG8FM_001119"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS5093796.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3981..4631)
                     /locus_tag="ACG8FM_001120"
     CDS             complement(3981..4631)
                     /locus_tag="ACG8FM_001120"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS5093797.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4641..4952)
                     /gene="yhbY"
                     /locus_tag="ACG8FM_001121"
     CDS             complement(4641..4952)
                     /gene="yhbY"
                     /locus_tag="ACG8FM_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS5093798.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4968..6077)
                     /gene="yqeH"
                     /locus_tag="ACG8FM_001122"
     CDS             complement(4968..6077)
                     /gene="yqeH"
                     /locus_tag="ACG8FM_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS5093799.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6074..6601)
                     /locus_tag="ACG8FM_001123"
     CDS             complement(6074..6601)
                     /locus_tag="ACG8FM_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS5093800.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6781..7566)
                     /locus_tag="ACG8FM_001124"
     CDS             complement(6781..7566)
                     /locus_tag="ACG8FM_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS5093801.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7559..8428)
                     /gene="accD"
                     /locus_tag="ACG8FM_001125"
     CDS             complement(7559..8428)
                     /gene="accD"
                     /locus_tag="ACG8FM_001125"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS5093802.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8429..9808)
                     /locus_tag="ACG8FM_001126"
     CDS             complement(8429..9808)
                     /locus_tag="ACG8FM_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS5093803.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9816..10235)
                     /gene="fabZ"
                     /locus_tag="ACG8FM_001127"
     CDS             complement(9816..10235)
                     /gene="fabZ"
                     /locus_tag="ACG8FM_001127"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5093804.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10260..10736)
                     /gene="accB"
                     /locus_tag="ACG8FM_001128"
     CDS             complement(10260..10736)
                     /gene="accB"
                     /locus_tag="ACG8FM_001128"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS5093805.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10739..11974)
                     /gene="fabF"
                     /locus_tag="ACG8FM_001129"
     CDS             complement(10739..11974)
                     /gene="fabF"
                     /locus_tag="ACG8FM_001129"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS5093806.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(11993..12730)
                     /gene="fabG"
                     /locus_tag="ACG8FM_001130"
     CDS             complement(11993..12730)
                     /gene="fabG"
                     /locus_tag="ACG8FM_001130"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS5093807.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12742..13659)
                     /gene="fabD"
                     /locus_tag="ACG8FM_001131"
     CDS             complement(12742..13659)
                     /gene="fabD"
                     /locus_tag="ACG8FM_001131"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5093808.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13766..13990)
                     /locus_tag="ACG8FM_001132"
     CDS             complement(13766..13990)
                     /locus_tag="ACG8FM_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5093809.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14043..15005)
                     /locus_tag="ACG8FM_001133"
     CDS             complement(14043..15005)
                     /locus_tag="ACG8FM_001133"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS5093810.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15015..15455)
                     /locus_tag="ACG8FM_001134"
     CDS             complement(15015..15455)
                     /locus_tag="ACG8FM_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5093811.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15761..16726
                     /locus_tag="ACG8FM_001135"
     CDS             15761..16726
                     /locus_tag="ACG8FM_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5093812.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(16783..17013)
                     /locus_tag="ACG8FM_001136"
     CDS             complement(16783..17013)
                     /locus_tag="ACG8FM_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS5093813.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17099..17986)
                     /locus_tag="ACG8FM_001137"
     CDS             complement(17099..17986)
                     /locus_tag="ACG8FM_001137"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5093814.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18075..19298)
                     /locus_tag="ACG8FM_001138"
     CDS             complement(18075..19298)
                     /locus_tag="ACG8FM_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5093815.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(19315..20361)
                     /locus_tag="ACG8FM_001139"
     CDS             complement(19315..20361)
                     /locus_tag="ACG8FM_001139"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5093816.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(20525..22252)
                     /gene="ptsP"
                     /locus_tag="ACG8FM_001140"
     CDS             complement(20525..22252)
                     /gene="ptsP"
                     /locus_tag="ACG8FM_001140"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS5093817.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(22252..22518)
                     /locus_tag="ACG8FM_001141"
     CDS             complement(22252..22518)
                     /locus_tag="ACG8FM_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS5093818.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            22997..25231
                     /locus_tag="ACG8FM_001142"
     CDS             22997..25231
                     /locus_tag="ACG8FM_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5093819.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            25379..25699
                     /locus_tag="ACG8FM_001143"
     CDS             25379..25699
                     /locus_tag="ACG8FM_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5093820.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         7197 a         5623 c         4481 g         8541 t
ORIGIN      
        1 tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta aaactggttg
       61 cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga tcttccagtg
      121 catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc tcgttttgag
      181 ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt aatgcttctt
      241 gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct tcttccatta
      301 catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca gcgtcttctc
      361 ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg gaaacaccca
      421 tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga ttcacattgt
      481 tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca tagcgcaatt
      541 cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca ataatatgtt
      601 ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg tttgcatgcg
      661 gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt gcataaatcc
      721 ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat ttacgtccca
      781 tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc tgttccatta
      841 tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc ttctcatttt
      901 tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg aatattgcct
      961 cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc ctcttttttt
     1021 ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct tccagcatcg
     1081 gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc ttgctttttg
     1141 atattcagca agacataaca agataatcgt tggatccgtg tccatgtata tctttttgtt
     1201 tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt tttcatgcgc
     1261 gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt agaagataac
     1321 aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca agttgaaaga
     1381 tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc taacgcatgg
     1441 cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg atagcctgca
     1501 cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt ttcctttgca
     1561 taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc gggatacaga
     1621 ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga cagttcttga
     1681 aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact gctgtaatca
     1741 aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt ttgcaagatt
     1801 tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac gggaagctcc
     1861 actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt atcgatgata
     1921 gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac ctctgctcct
     1981 gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg attgtactct
     2041 acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata aaaaagaaat
     2101 agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata aactattctc
     2161 gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct atatcgttcg
     2221 gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag gcaaaaccgc
     2281 tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc ttcgtctgta
     2341 aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct taaataactt
     2401 tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg accagaccct
     2461 tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc gcttcctgga
     2521 taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata ttctggaaaa
     2581 acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt ttcttctaac
     2641 gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat atagcacagc
     2701 gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc gagcatattg
     2761 ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc aatcatcaac
     2821 atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa ggcaaccgct
     2881 aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg taaatgacgt
     2941 ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc gtaaatgaaa
     3001 gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac tagcggagca
     3061 tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa aacatgtacg
     3121 atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc aatacgtttt
     3181 acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc gttgatttga
     3241 cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac atttcgaacg
     3301 tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc aatttttaat
     3361 tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt tttgctaccc
     3421 agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca agcaaatggg
     3481 ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat gcaatggctc
     3541 gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct acataaatga
     3601 ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt gcatgaagaa
     3661 tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca agaccatgcc
     3721 agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg attaccattt
     3781 tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca atactagctt
     3841 tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct actccaagca
     3901 catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg ttttctctag
     3961 aataagcagt atattcatta ctatagtcca tattcataca acccttcatt ttgaatataa
     4021 tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat cttttgacgt
     4081 attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg atatggagta
     4141 tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt cagctcatcg
     4201 attttatacc attttggcaa atattctacc atatccccac cgataataaa gtaataatct
     4261 gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact tttcccacca
     4321 cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc taattccaac
     4381 atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg tggctgataa
     4441 gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc agccatcact
     4501 aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg ctttttcttc
     4561 tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt cttagcttgc
     4621 gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct ttgatacttt
     4681 tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac gatttcgcaa
     4741 tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt attttgaagt
     4801 aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac gatcattgct
     4861 gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc tttgctacgt
     4921 aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta aatcaatgct
     4981 ttgcgtttca atacatctac accctttggc gcccagcccg caattactac tggcgctgtt
     5041 acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac agaaaactca
     5101 aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg taataatcca
     5161 cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg aatatttaca
     5221 tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc aaatcttgca
     5281 agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg tttgatttct
     5341 tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat ttgatggcga
     5401 tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat ctcgatttta
     5461 tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc cgctgtattt
     5521 ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac gatatagaca
     5581 tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc atgagatttc
     5641 ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc acgttccttc
     5701 atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac tttgttccca
     5761 actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag tgaaccattg
     5821 aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa ttcattcagt
     5881 aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata atggcgtaat
     5941 ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa agccgtttgc
     6001 ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca gccgatacat
     6061 tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt tttgctaata
     6121 gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa ctatctgttg
     6181 cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca cgtatatcag
     6241 tcataatctg atcgcctacc attactatct catcatctgc taaatccaat tgtttctttg
     6301 ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa agatcaaatt
     6361 tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca acagggattc
     6421 ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct ggattattcc
     6481 atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga ttctttaatt
     6541 gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa tttgaaaaca
     6601 tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca accccgtatt
     6661 atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag aaaaagcatt
     6721 tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa caaaaaacaa
     6781 ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat ctaatcgaac
     6841 aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt tatatcgtgg
     6901 caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta actcgcgtag
     6961 ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac cgtctttcca
     7021 caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt ccagcatcca
     7081 gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac caatgatgat
     7141 ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa tagcctctcc
     7201 ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga tcaaggtaat
     7261 caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc gatagccttc
     7321 aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc ctttttgaat
     7381 accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa tcgcttcgtc
     7441 atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct cttcaaaaaa
     7501 atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat gtgctgtctt
     7561 atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg attttctgtt
     7621 tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag aattcagcct
     7681 tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt cttccagcaa
     7741 aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca aaactggcag
     7801 ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct tcattgctat
     7861 gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt ccttcttgca
     7921 tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt gttgctcgtt
     7981 cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg ataaaattag
     8041 gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca gtgaggactg
     8101 cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat tcgggaaatg
     8161 ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt tcatcaataa
     8221 tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga gggcatactt
     8281 tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac tttgcccaca
     8341 tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga ttgatccgaa
     8401 tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt tctggtgtcc
     8461 aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct atgactgcag
     8521 gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct gtgataaatt
     8581 cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca tggacgatta
     8641 tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa actgcactgt
     8701 ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc cctggagaag
     8761 gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg tgtccttgga
     8821 atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc acctgttttt
     8881 ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg attcgagtat
     8941 tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc gttccggcat
     9001 tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct tctctttttg
     9061 tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg cgttgtaaag
     9121 aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag atctgaactt
     9181 ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt ccaaatgccg
     9241 ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt gattgaactt
     9301 tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga taaccaattc
     9361 tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt cctggaatga
     9421 ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc attgcatcga
     9481 tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg gaggcaaatc
     9541 ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt gtcacgattg
     9601 cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc gggccaatgc
     9661 agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct gaatatatcg
     9721 ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg atttcaccgc
     9781 ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa ggttaattcc
     9841 gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc ggctgaagct
     9901 ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac tttcttacgg
     9961 aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg ttcaagagaa
    10021 agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc tggcataaca
    10081 ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt tttcttagca
    10141 acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat tggataacga
    10201 tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc ctgctttgct
    10261 tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc gttttctaca
    10321 aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag tttcatcgct
    10381 tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt gaaagctggt
    10441 ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac ctcgattcca
    10501 tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc ttgttcaggt
    10561 atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat attcatgcct
    10621 cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa atcgaattcc
    10681 gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat atccaaaatt
    10741 attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct aatgaattac
    10801 tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc aagtcaatct
    10861 caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct aatgccagca
    10921 aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc atcgatttcg
    10981 tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt gcttttgctt
    11041 ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg acttgttccg
    11101 gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca gaaccatctg
    11161 gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg atttctccat
    11221 agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga acgcctgctc
    11281 cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca cgattaggat
    11341 cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc tcacaaatcg
    11401 ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg ttacggaatg
    11461 cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta caaatacctt
    11521 ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc attggtacga
    11581 acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg ttttcgattg
    11641 ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga ttcatttttt
    11701 ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca tagatcgaaa
    11761 acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg aaatctttga
    11821 cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc ggtgcgatgc
    11881 cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca ataggtgaag
    11941 tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt tcttatccgt
    12001 gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga ttttgtgcta
    12061 agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc ggaatttgct
    12121 tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt gtgatgaatc
    12181 ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc acagattttg
    12241 ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata tttccaatca
    12301 acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc atttttttca
    12361 atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat tttttgaaat
    12421 cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg tttactaaca
    12481 catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct ttctcataat
    12541 cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt tcttctatct
    12601 tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct tttgcaaaag
    12661 ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca ttttttcctt
    12721 ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg tctgcttatt
    12781 ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc ttaatacttt
    12841 tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat ggatactctc
    12901 atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg tttctttctt
    12961 cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga attgaatctg
    13021 acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat gaaaaggccc
    13081 gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta aaacagcttc
    13141 gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat aatttgcagg
    13201 agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt caatcggcgc
    13261 attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca tataagcgcc
    13321 tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag ctactaatgc
    13381 cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat gctccttcaa
    13441 taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt gcgtctgatc
    13501 caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa tctcacttgc
    13561 ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca taccttgata
    13621 ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca atatatccag
    13681 aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc aggaaatgtc
    13741 ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa gtcactaaat
    13801 cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca tcttcaattt
    13861 cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg atgtcagtag
    13921 tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct tgaatttttt
    13981 cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta taatttaaca
    14041 gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc tgtaaatatt
    14101 actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc caataagata
    14161 gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa cttttcttgc
    14221 ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg atgcaaaagc
    14281 agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac gtctcgtacg
    14341 gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc tgaatcttct
    14401 gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact tctggtaccg
    14461 tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa gagaatccca
    14521 cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat tagcttagat
    14581 agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat tttttcagcc
    14641 atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc gatagcatga
    14701 gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta atcgggcgac
    14761 attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg ttttatcaat
    14821 ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca tcttcgttca
    14881 cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt ggaaagatca
    14941 ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat tccaaattgt
    15001 tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt cagtgcatcc
    15061 agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac catttcacga
    15121 tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc caactttaca
    15181 acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac taacttgtta
    15241 atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc tgaagttgtt
    15301 tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga cagatctttg
    15361 aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa gacactaaca
    15421 agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact aattagtttg
    15481 attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg caaccttaaa
    15541 agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa acaactgata
    15601 aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat tttgattatc
    15661 aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt atttcaaaat
    15721 ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag aaaaaatgaa
    15781 aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta aggtcatcta
    15841 tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc agaataaaca
    15901 gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt tcgtcgtagt
    15961 agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt accgtccttt
    16021 ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta ccaataaggg
    16081 atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc tctataaaaa
    16141 aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt tgtctgaaat
    16201 tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt ttgaacagaa
    16261 aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga cttacttgaa
    16321 aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta tgcttcaaaa
    16381 agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc agtttttaag
    16441 aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga aaaagcacta
    16501 cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg tttctcctga
    16561 cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt caaaagaaga
    16621 aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag aacagtttga
    16681 aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc ttctcggctt
    16741 cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga ttaagtcata
    16801 gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag tatctgtttc
    16861 agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct ctacgccttc
    16921 tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag cctccagtcg
    16981 ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc ttatttctgt
    17041 tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa ctcgtttttt
    17101 atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa gcttgggttc
    17161 cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct tgggtagttg
    17221 ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa tctttgataa
    17281 aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga gaataattgc
    17341 ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc tttaaatcaa
    17401 gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta cctgcaatca
    17461 tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc aatgtaaatg
    17521 tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct tctccaccga
    17581 ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc ggtgcatcta
    17641 acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg cttggtgtac
    17701 ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc cccgactgtc
    17761 catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga atattacttt
    17821 gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact aaaggatcag
    17881 cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt agatggcgtg
    17941 cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt tcgacctcct
    18001 aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat tttttcataa
    18061 aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg ttcaatcgag
    18121 tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc ttgataaaat
    18181 tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc ttctaaaact
    18241 tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc aaagtcttcg
    18301 tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa ataagcattt
    18361 ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag cccttgtgtt
    18421 tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc tacatcattg
    18481 ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga gatcaattcc
    18541 tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt aggataagcg
    18601 gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata agaaatcctg
    18661 cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct taatttgctg
    18721 atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc agtaggaatc
    18781 acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg ttcacttgga
    18841 caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac atgggattgc
    18901 ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt atggtaagta
    18961 tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc tgctccgaat
    19021 tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata agcataccac
    19081 atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact tggcattcga
    19141 atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat atagacttca
    19201 tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac tccgctgact
    19261 tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc caaactagtc
    19321 cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta cactttcgta
    19381 tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg tttgataacc
    19441 ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc ggataaattc
    19501 ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat tatacactgg
    19561 aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa cgatcccttc
    19621 tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac cttctatgat
    19681 ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt ttacaatttt
    19741 ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa atgtgtattc
    19801 gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga aaaacaaacc
    19861 gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga acttttgttc
    19921 tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag cttgaatcgg
    19981 cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg tgatcagata
    20041 gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa gctggttcga
    20101 tctgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga caactttttt
    20161 tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg tattgatgtg
    20221 caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt ccgtcagagc
    20281 acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct tttcactttc
    20341 aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata acatagccta
    20401 caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa aaacgttttc
    20461 ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc ttttgatacg
    20521 ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata tcgcattctt
    20581 tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc attaaactac
    20641 gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata cctactagca
    20701 atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat tttccttcag
    20761 cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta tatggttggt
    20821 acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga atcaaatcgt
    20881 tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag actgctgctg
    20941 ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg ccttcactta
    21001 tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct ttgagtgtag
    21061 ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc aatgcacgca
    21121 tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat gcacgatatc
    21181 ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta tctccaccga
    21241 tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca gctttgtaag
    21301 cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg aattcagtac
    21361 ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct tttggtgttc
    21421 cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct gcatttttta
    21481 atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca aattcagctt
    21541 tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca gccaaaatat
    21601 cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga atttctaaag
    21661 aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca aacgctttta
    21721 caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca acaacgacca
    21781 cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc gctaaaatac
    21841 gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta tcttccattg
    21901 cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca gcatttacgc
    21961 tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa agaaccatca
    22021 aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct ttttcacgaa
    22081 tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct tcttcagccg
    22141 cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc aggtaagctt
    22201 ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc attattcaga
    22261 taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt cgtcagcacc
    22321 ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa cgcccatgat
    22381 tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt taaatttgct
    22441 tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg ctactacgtg
    22501 aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt ttatgtgagg
    22561 gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat ctgtaaagat
    22621 taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa agtattcaaa
    22681 ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg tttatttcct
    22741 ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac cctctttgtt
    22801 ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag agtcaaaggt
    22861 caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa aaaactaggg
    22921 gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca tttttgtgaa
    22981 aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa cgattcactt
    23041 gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct gttatcaaaa
    23101 aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc catttggatt
    23161 tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc aaggaaatta
    23221 tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg gtcagccacc
    23281 tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag aacaagcacg
    23341 acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag tcattgaaat
    23401 tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg ttggtaaaac
    23461 agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc aaaaattaat
    23521 ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg gtatacgagg
    23581 acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag aaaatgtgat
    23641 cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg atggaaatat
    23701 ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa tggttggtgc
    23761 tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac gtcgtatgca
    23821 acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga aaggtttgca
    23881 aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg aagcagccgc
    23941 aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca tcgatttgct
    24001 agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta aaacaatcga
    24061 gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag aagatttcga
    24121 aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag aacgtcagct
    24181 cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg ttgaacaacg
    24241 gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga aaaacttagc
    24301 agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag tagcgaaagc
    24361 gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct ccttcttatt
    24421 tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct acgaactatt
    24481 cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa aacatagtgt
    24541 ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg gtcaattaac
    24601 agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag aaaaagctca
    24661 tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga cagatgcaca
    24721 aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg ccggaactgg
    24781 taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc gttctgtatt
    24841 gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg ggatcatcga
    24901 attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc tagacgatgt
    24961 caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg tcaaagaaaa
    25021 attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac gcacgatcca
    25081 agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta tccatgagtt
    25141 gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag aacgtttggc
    25201 taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga gacggtattt
    25261 ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt cattttataa
    25321 aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg tgagcgccat
    25381 gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc ttgaaaacac
    25441 tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct acactgaagc
    25501 ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc cgaaagaaat
    25561 cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc acgaggatga
    25621 aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac atgaattagg
    25681 cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt gggacaaaag
    25741 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
    25801 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa at
//
LOCUS       DAZUMT010000030        25680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000030 DAZUMT010000000
VERSION     DAZUMT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.030"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1127
                     /locus_tag="ACG8FM_001144"
     CDS             279..1127
                     /locus_tag="ACG8FM_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS5093821.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="ACG8FM_001145"
     CDS             complement(1298..1639)
                     /locus_tag="ACG8FM_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFS5093822.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="ACG8FM_001146"
     CDS             1725..2432
                     /locus_tag="ACG8FM_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5093823.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="ACG8FM_001147"
     CDS             2419..2742
                     /locus_tag="ACG8FM_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5093824.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="ACG8FM_001148"
     CDS             complement(2831..3868)
                     /locus_tag="ACG8FM_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5093825.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="ACG8FM_001149"
     CDS             complement(4315..5298)
                     /locus_tag="ACG8FM_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS5093826.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="ACG8FM_001150"
     CDS             complement(5714..6484)
                     /locus_tag="ACG8FM_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5093827.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="ACG8FM_001151"
     CDS             complement(6477..7412)
                     /locus_tag="ACG8FM_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5093828.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="ACG8FM_001152"
     CDS             complement(8006..9655)
                     /locus_tag="ACG8FM_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5093829.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="ACG8FM_001153"
     CDS             complement(9850..10746)
                     /locus_tag="ACG8FM_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5093830.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="ACG8FM_001154"
     CDS             complement(10739..11419)
                     /locus_tag="ACG8FM_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5093831.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="ACG8FM_001155"
     CDS             complement(11420..12202)
                     /locus_tag="ACG8FM_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5093832.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="ACG8FM_001156"
     CDS             complement(12195..13112)
                     /locus_tag="ACG8FM_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5093833.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="ACG8FM_001157"
     CDS             complement(13115..13399)
                     /locus_tag="ACG8FM_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093834.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            14187..17414
                     /gene="fss3"
                     /locus_tag="ACG8FM_001158"
     CDS             14187..17414
                     /gene="fss3"
                     /locus_tag="ACG8FM_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS5093835.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            17526..17840
                     /locus_tag="ACG8FM_001159"
     CDS             17526..17840
                     /locus_tag="ACG8FM_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5093836.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17853..18227
                     /locus_tag="ACG8FM_001160"
     CDS             17853..18227
                     /locus_tag="ACG8FM_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5093837.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            18237..18581
                     /locus_tag="ACG8FM_001161"
     CDS             18237..18581
                     /locus_tag="ACG8FM_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS5093838.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            18650..19666
                     /locus_tag="ACG8FM_001162"
     CDS             18650..19666
                     /locus_tag="ACG8FM_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5093839.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            20260..22134
                     /gene="ltrA"
                     /locus_tag="ACG8FM_001163"
     CDS             20260..22134
                     /gene="ltrA"
                     /locus_tag="ACG8FM_001163"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5093840.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            22236..22616
                     /locus_tag="ACG8FM_001164"
                     /pseudo
     CDS             22236..22616
                     /locus_tag="ACG8FM_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            22661..23803
                     /gene="dcm"
                     /locus_tag="ACG8FM_001165"
     CDS             22661..23803
                     /gene="dcm"
                     /locus_tag="ACG8FM_001165"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFS5093841.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            23793..24082
                     /locus_tag="ACG8FM_001166"
                     /pseudo
     CDS             23793..24082
                     /locus_tag="ACG8FM_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24338..25522
                     /gene="mobT"
                     /locus_tag="ACG8FM_001167"
     CDS             24338..25522
                     /gene="mobT"
                     /locus_tag="ACG8FM_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5093842.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            25519..25632
                     /locus_tag="ACG8FM_001168"
     CDS             25519..25632
                     /locus_tag="ACG8FM_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5093843.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         8057 a         4994 c         5032 g         7597 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt ttcttcgtat
    13921 cattgaataa gtttgaaaag ccttgattta taaaggtttt tccgtaaata tattttgtta
    13981 tatttgatta aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa aggcagtata
    14041 tttcgtattt tatattaaat ttttaggctc attcattctc aaatccattc tatttagtca
    14101 ataaatattt taaaaagact ttataaacaa agatgttcaa gcgagcatct tatttttttg
    14161 tgcctaattt gaaaggagaa catttcatgt ataaaaaaca aaaaaagaaa ttatgtgtat
    14221 taactatttt gagtttggct gttcaatcaa ttgtaggagc aattgctcct acgattgctt
    14281 ttgctgatga aattacccat ccacaaacgg taaccgtaga actagattta gcacatcaat
    14341 atgctgttga aggaactttt agtgatggtc gtccaatgtc agaagtcact gttccgcatt
    14401 atgcagttta caatggtgtg aagcaagata ttttctgtgt tgaaccagga gtcccgattt
    14461 acaatgagtt cactcctgga tatgagaaga acccgttacc agatatgtcc gataaagcca
    14521 aattagtttc cgttttatgg aaaaaagtag gtacagatgc agatactcat attgtggcac
    14581 agaagatgat ctggcaagaa gttaatgggt atacccttca ctcaataaaa cgatcagatg
    14641 gtagtgccgt aaatatcgca gccattgagg caaaaatcaa tcaagccatt gccgattatc
    14701 agaagaaacc aagcttccat aatagtacgg ctaaaacagt attagggcaa tcgactactg
    14761 tgacggatac gaataatctg aatttgtcag agttcgatga ggttgtggaa aataccgcaa
    14821 acattgatta tcgtgtgaat ggcaatcagt tgataatcag cccaaatgcc aattcaaaag
    14881 aaagtggtgt gttgacgctt aagaaatctg ctggtaccgg aacacctgtc gcctataaaa
    14941 aggctggcca acagacttta atggccgggg cgatcgataa gccgaacact tatacggtca
    15001 aaattgatgt ggaaatggag ggttctttga agattaaaaa agttgataaa gaatcgggtg
    15061 atattatacc aggaacggtt ttccatttag actttggaaa aactttaccc gcaaaagacg
    15121 tgaccaccga taaagaaggc attgcgacct tagatgagat tcctcatggt acaaaggtaa
    15181 ccattactga aaaatcggtg ccggcgcctt atacgattga taccacaccc atgactgcca
    15241 caattaaagc aggtgaaacc atttcagtga cttcaaaaaa tacgcgagaa aaaggtcaaa
    15301 ttattctgga taaaaccgga attgaaacag ggagtgatct ttggaacgat aattactctt
    15361 tagcgggcaa tacgtttgcc attcgcaagg atagcccaac tggtgaaatc gtccaagaaa
    15421 tgaccacgga tgaaaaaggt catgcagaaa caccaaaaga gattgccaat gcgttggaat
    15481 tgggaaccta ctatgtgact gaaaccaagg ctagtcatgg ctttgtgaat actttcaaac
    15541 cagtaaaagt cgaattgaat tatgtcaatc aaacagtggc tcttgttacc agtaacgtaa
    15601 aagggcaaaa ccaagaaatt actggtgaaa ccactttgac aaaagaagac aaagataccg
    15661 gtcatgaaac tcaagggaaa gctgagttta aaagagctga atacaccttg tttactgtta
    15721 aggatggaaa agcggtcaaa tggaccgaga actttaaacc agaattgttg aagggaacga
    15781 aagcttctga tgaaaccgta actttgactt tagatgaaaa gaatcaagtt gccgttaaac
    15841 acttagcgat taatgagtat tactggcaag aaaccaaagc gcctgaagga tataccttgg
    15901 atgaaacaaa gtatcctgta tccatcaaaa aggtggatga tagcgaaaaa aatgctgtga
    15961 ttactcgaga tgttgcggca aaagaacaag tgattcgctt tggctttgat ttctttaaat
    16021 ttgctggatc aactactggc actgccgaaa ctggcttcaa tgatctaacc tttaaagtgt
    16081 cgccattgga aggaaccaat gaaatcaccg gtgccgaaga tgaagcgacc acagcttata
    16141 acgagcaatt agggtttgat ggttatggca agtttgaaaa tcttccttat ggggattatt
    16201 tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat tacgccatta gaaatccgtt
    16261 ctacttttaa agaaaataaa gaggattata ccaagagtga ttatatcttt actattaccg
    16321 aaaaagggca aaagcaaccg attaaaacgg taacggtccc ttatgggaat ctaaccaatg
    16381 agaatttctc agtcagtttg aaccgtttga tgctttatga tttgcctgag gaagaagata
    16441 gcttaacttc tcttgcgact tggaaagaag gagataaaga attgactact cttgattcta
    16501 ccgagctagt cgataaattg agctataatc tacatgaaat caaagaagac tggtatatcg
    16561 tagctcaagc catcgatgta gatgctacaa aagctgccca agaaaaagat aaaaaagcca
    16621 aaccggtggc gattgcggaa acaaccgcaa ctttagcgaa taaagagaaa acaggaactt
    16681 gggagattct gcataaatta actgtcgaac aagttttaga caagaccatt gtcttattca
    16741 actatgtgta tgaaagcaaa gaagcctatg aagctggtga tgagccggta gcgaaggatg
    16801 ctagcttgaa caatcaagcg caaactgtta attgtatggt ggaacgtcat gtttccatcc
    16861 aaaccaaagc gcacttagaa gaaggttctc agacctttac tcatggtgac gtggtagata
    16921 tgtttgatga tgtatccatt acccatgatg tactagatgg ctcaaaagaa gcttttgaaa
    16981 caattctgta cgctttattt ccagatggta caagtaaaga aatttggaaa tctggcaaga
    17041 ttgattacga agtgaatgac aaagaattta ccaaaaccgt acttgcggaa aaagtggata
    17101 caagtaaata tccagaaggt acaagtttta ccttcaaaga aattaactat gacaaagatg
    17161 ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa atctcaaact ttaacgccca
    17221 aaaaagttcc gacaacacca agtacaccag aacaatctga aatgccaact gttccaagtg
    17281 gctctcaaga atctagtcct acagtgaagg cattccctca aaccggggag aaaacttcca
    17341 atgtgctact gttcattggc tttagcttga tctttgcgac ggctggctat tatttttgga
    17401 atcgtcggaa ctaaggtgat aagatgaaac atgtaaatag tagaaaacaa tacgcgcgac
    17461 cgcctcctaa aaaagtggtc gcgctatttt tgtattcaaa actaacaatg aaaagaggaa
    17521 aaaatatgga attgaaattt gttgtaccag atatggtcga gacgttcgga aaactaacat
    17581 acggtggtga aggtgaagtt ctatcggaag ggtatggtcg aaatactaca attgttggtc
    17641 gaagttacca tctgttttca aataaacaac gggccgatga tattgaagtg gtggtagctg
    17701 cggaagccgg cgaaaaggat tttgaccaag atcaaccact aaaagctgta aatccccact
    17761 taattgccaa aggctatgaa attgagaatc gtgggtttac cgattacgtg ttgtatgttg
    17821 atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt attgtcgtag
    17881 attcaggatt aacctttggc aagttgcgtt tttccgcatt acgtcgagaa gtacgcaagc
    17941 aaaatgagga cggaacggtt agtaacgagg taaaagaacg gacctataat ttgaaatcct
    18001 ccgcgcaagg tcgaatgatc caagtcagta ttccagcaaa tgtaccctta cgcgagtttg
    18061 cttatgatgc agaggtagag ctggtcaatc caatcgtgga tacggtcgcc aattatgttt
    18121 ttcgggaagg aaccaccgtt aattggttta tcaaggcaga tgatttagtc ttgaaacgac
    18181 aactgaatca aggaaaacct acgaaccaaa atgaaggaaa gaaataggtg aagaatatgg
    18241 aaattaatgt tgaacatatt ttagattgtc tcgatcaatt tggcaaaggt gagctaacgg
    18301 aggaacaact cactaacgca ttaacctatg atgaaaaaat gtttctgatt atgcatcaag
    18361 ggttactgga agtaggtaac gatgccaaag aagactttga tgtactggat tggttagggg
    18421 aagaagagtc tttctttatg gttgtcgagg taaatgaaag tttatgtcgg caggcagaat
    18481 ctgttttgga agaaatcgat gtggagatgc cggatgccat cgaaagattt ttaaaacaat
    18541 tagtggaaac gaaacaactt cccgtagtag taaatgacta gcataacttt ttgaaattgc
    18601 ccaatgaaat cctattgggc tttttcgact ggaggtggaa accatcaaaa tgtacaaagg
    18661 acatcgcata cgcgcaggag atcaacactt gatttatcac tttgttcttg gctggctaat
    18721 ctttttgttc attggttgga tgagcgtctt ttatttacaa gaacttagac aatttgatat
    18781 ttctaaggta tcattttcca ctagtgaaat tggctggtct atgaaggatg taatctgctt
    18841 gctaggaagt ctcgctcttt caggaagcat gatgttactt tatatacact tttttcagga
    18901 tcattggcga agtctttggc atcgacaaaa gttagctcgg atgattctag aaaaccattg
    18961 gtatgaagta aagcagactc aaggtgaagg ttttttcaaa gatttgaata gtagttggac
    19021 caaagagagt atcagttact tccccaaaat ttattatcga atgaaagatg gcttactttc
    19081 tattcgcgtt caaatttcac tgggaaaata tcaggatcag ctattgaagt tggaaaataa
    19141 gttagaaagc ggactgtatt gtgagttagt agaaaaagaa ctcaaagact cctatgtgga
    19201 atacaccttg ttatacgata tgattgccaa tcgaattggg atagaagaaa tagtggcaga
    19261 aaatggggcg ttgcgattga tgaaaaatca agtatgggcg tatgattcct tgccgcatat
    19321 gttaattgct ggcggcacag gtggtgggaa gacctatttc ttactcacca ttattcaagc
    19381 actattgaag tcagatgcag aattgtttat cctcgatccc aaaaatgcgg acctcgccga
    19441 tttaggtacg ataatgcctc acgtatactc ccaaaaagaa gaaatttctg aatgtgtgga
    19501 agatttttac gaacgcatga tagctcgtag taggtcaatg aaagaaatgc ccaactacaa
    19561 aacaggagag aattatgcgt atctcggact tccgcccaac tttttaatct ttgatgaata
    19621 cgtggcttat atgggtgcga atcgttttcc tacaagcatc gaatagatgt gaaatggaag
    19681 cctattgaga ttatcaatag tgacaactaa tgttttgaga gatggaatga aagccttcaa
    19741 gttgagctga aacatccccg ctaatactcc tacatgctct aatcaatgaa gtagattggt
    19801 tttggtatga agctaggtga agtcgtctga aatctgccta agtgcttgag cataaggctg
    19861 actatagggc gggcggctga aaaagataac tatggtaaga atgtctgaaa atgactgacg
    19921 aatctgcgaa tgtacgggtc taaaaggctg atcttattga aagataggaa catacggacg
    19981 tatggtgaga taccgacaag taaggttcca tgatatgaaa atcgggatag ctaacgaaga
    20041 agctataatt tcacaggctc atagtagata cctaaggtta tacatgatag ctaaaaacat
    20101 tggaacgatg taagcagaaa actatcaatt aaatgactac ttctagatag gtgcatataa
    20161 gtaataacga aattgctttg tttctgtgaa agtaagggca cagtaccgat gaagcgtcta
    20221 acaaatgtgg agggatagcc ctaagtctat ttaataacaa tgaatgttga aacagatgaa
    20281 gttcaaggat ctggtaagaa cgtgggaaaa tccgttatgg aggtgccacg cttgaacaca
    20341 aaaatacgtt actgggaata ttatgatcta caagaaacat tcgatcagct atttactcaa
    20401 agtagaaacg ggaaaaagtt ttatcaatta tatgagttaa tcatttctga aaataatatt
    20461 cttttagcat accgtactat caaggcaaac aaggggtctt cgacaccagg aacggattcc
    20521 ttcacaattg ataactataa ggaaatgaac caagcagagt ttatccatct tattctcagt
    20581 catttagaaa attacaaacc aaaatccata aagcgagtaa tgatacctaa acccaatggt
    20641 gagaaacgcc cattaggaat tccatgcatg attgatcgaa tgatccaaca gatgttcaag
    20701 cagatactgg agccaatatg tgaagctaag ttttatgaac atagctacgg tttcagacca
    20761 ttgagaagtg caaagcatgc tctaggtcgc attatgtatc tcataaacat cagtaaaatg
    20821 cactatgcag tagacattga tatcaaaggt ttctttgata atgtaaacca ccgtttactg
    20881 ataaaacaat tatggaatat aggaatttgt gacaaacaag tcttagctat tctttcaaaa
    20941 tctctgaaat cacccattca aggagaaggt atcccgagca aaggaacgat ccaaggaggt
    21001 atcatttctc cactattatc taatgttgtt ctaaacgact tagaccattg ggtctcaaag
    21061 cagtggcata cctttgaaac aaagtatccc tatactaaag gttacaataa atttcgtgct
    21121 ctaagagata caaatctaaa gcaaggctat attgtcaggt acgcagatga ctttaaaatc
    21181 atgactaatg attatccgac agcattaaaa tggttccacg ctgtcaagct ttacctaaaa
    21241 gacagactta aattagatat ttccaatgag aagtctaaaa tcgtcaattt gcgtaaatgt
    21301 aagtcggaat ttttaggatt cgctatttgc gtgaaacaaa aagggaaaaa atgggtttgt
    21361 aattcacgta tttctaacaa aaaaaaggat cagattaaag aagagattaa acaaagaatc
    21421 aaagatattc aaaagagtcc tacagcacaa aatgctctcc tgtttaatgc tctcgtgctc
    21481 ggtgttcata attatttcag gtatgccact catattaatt tagatttgaa taggatgaac
    21541 tacgttcttt cattcactac ttacaaccga ttatcaaatt gtagtacaag atgttacccc
    21601 aacaaagctt ccccaactta caaaaagttt tactcaacaa cgatgaaaac gtataaagta
    21661 gctggagtct atttgtaccc tctttgcgac gtatctacaa aaacaattta cggatttaat
    21721 acggaggata caccgtttac tccgtttggt cgtcaaagat tggagcacaa aagcctacag
    21781 tctcttgtgt tccaagaatt aagaaaattg atggaatcga aaataccgaa tagaacggtt
    21841 gagtatttag atagtagaat atctcgttac tcaatgaaat caggcaagtg tgagattaca
    21901 aaacaatttt tgccagcaaa agccgttcac tgtcatcact atttgccaaa aagtcttggt
    21961 ggagacgaca aattcgataa cttgagaatt attcataagg atatccatct tctgatccat
    22021 acaacaaata aaatgataat cgatcactac gtgcatgaac tgaaactggt gccagagcaa
    22081 atagcaaaaa tcaaccttta tcgaaagatg tgtaatctgc aaaacattca atagaaaata
    22141 aatagatgga acgccgtatg aggtgaaagt ctcacgtacg gtgtggagcg ggggaaaaga
    22201 tggagattat atcaaattct tacctatcgc tatagatgtt aacgacaaaa gaaagtgcgg
    22261 tgattttgaa taagcttaaa caaatcgtga tgttaggccg tcaatctggt ttctttctga
    22321 ttctagcttg tcaaaggccg gatgcgaaat atctaggaga tgggattcgc gatcagttta
    22381 acttccgtgt ggctttgggt cgtatgagtg agcttgggta ttccatgatg tttggagaag
    22441 tcgataaaaa tttttttatg aaacgcatca aaggacgagg ttatgtagat acagggggca
    22501 gtgtgattag tgagttttat acgccacttg taccaaaagg gtatgatttc ttaagggaaa
    22561 ttagcaacat tgttggatta accgttcata cggaaagaga aaataatagt gtgtagccta
    22621 ggaatatcaa taatcctggg cttttttatt gaggtgattt atgacatttt tagatttatt
    22681 cgccgggatt ggtggctttc gcttaggtat ggaacaagcc ggtcaccagt gtatcggttt
    22741 ttgtgaaatt gatgaatttg ctcggaggag ttataaagca atccatgata caaggaagga
    22801 ggtggaaatg catgacatca caagtgtatc agacgaattt gttcgatccc tcggaccagt
    22861 ggacatcctt tgcggcggct ttccgtgcca agctttttca attgcgggaa agcggcaagg
    22921 attttccgat actcgaggta ctttattctt tgaaatcgct cggttcgcca ctcttctcca
    22981 acctaagttt ctattccttg agaacgtccg gggattgctc aatcacgaag gaggggctac
    23041 gttcgagacg atcctccgaa cgttggatgg attggggtat gatgtggaat ggcaagtgct
    23101 taactcaaag gcctacgttc cccaaagccg tgagcggatc ttccttatcg gacattctcg
    23161 agatgcatgt accgaacaag tatttcctat cattggatcg tcttcaacat ctgatcaaaa
    23221 catcagaaac ttggtaaata tcaatccttc caatcgagga atgggtggcc aagtctatgg
    23281 ttcagatagc gtagcaccaa ccttgaccgg tgatgaagga attaaaattg ccttaccggt
    23341 aaaggatggg atttctgtag cgggtatgtt gtcgggaaat tttgaacaag gcaataggat
    23401 ttatgaggta accggaacag cccccacttt gtcaacgaaa caagggggaa cgaagattat
    23461 gattcggaaa aatgaaacag aatatcaaga ggtgaagcca ggggacagtg tgaacctggc
    23521 ttttcctaat tccacaagtc gccgaggtcg tctgggaaaa caaagtgtgc ataccttact
    23581 aacaggagac caacaagcgg tagtcacgga tcagtaccag attcgaaagt taacacctag
    23641 ggagtgctgg cggcttcagg gctttccaga ttgggctttt gatcgagctt ctcaagtcaa
    23701 ttcagatagt cagttgtata aacaggccgg aaactcagta acagttccgg tcatttttga
    23761 tattgctaga agattaaagg gggtaaaagc atatgattta tgacgagttg attggagaaa
    23821 tctactggat gattggcaag attcaggcag atccgaaatt ggaagaagaa ctgtaccggc
    23881 tcaattttga tattcgcaaa aacggggtga aagtccaggt gattcgtatg tagtggatga
    23941 ggaaacggat gctcgaatgg agctcaatca agtcatcggt gaattggaac gaattgcgga
    24001 ccttgccaaa gaacctgata tccgccaata cttgtttgag ataaaagcag agttggaaat
    24061 tgagggtatc accgcggagt aatagggaaa taacccactg ataggcactg accgcggggg
    24121 atttgcgaag cagaaatcct ccgtggtgct gtgcctgttc atggacggcg gcagccggcc
    24181 atgatgtctc accccccgta tctaacaggg gggtacaaaa acacaaaaaa caagcaagta
    24241 accataaaaa atgcttgttg tatcaaggat attaagtaat ttgtataggt gttaactcat
    24301 ttggaaaagt taacaccttg aatttggagg tgaaaacttg gcgcaacgaa atttagatta
    24361 tcggttatta aaagaacgac gcaacgaata tggcgtttca caaaataaat tagctactgc
    24421 ttgcggactt agccgcccat acctaaatca gatagaaaac ggtggcgtaa tcgcctctac
    24481 aaagaccatg agaaaaattt ttgatcaact ggaaagtttc aatcccgatt tacctttaac
    24541 gttactattt gactatgtaa ggattcgctt tcccacaacg gatgtgcgga aaattattca
    24601 agaaattctc catttgaaat tcgagtatat gcttcatgaa gattacgcct tttactccta
    24661 tcaagaacaa tatgtcatgg gcgatattgt agtgatgtta tctcatgaag aagataaagg
    24721 tgtccttttg gaattaaaag gtcgtggttg ccggcagttt gaaacttttt tactcgccca
    24781 aaagcgtagc tggtacgact tttttgaaga ttgtctaaaa gccggtggcg tgatgaaacg
    24841 cttggattta gcaatcaatg atcgagtggg gctactggat attcctgatt taacgaaaaa
    24901 atgtcagaag gaagaatgta tctccttgtt tcgtaccttc aaaagttacc gttccgggga
    24961 actgttgaaa gctgatgaaa aggatggtat gggcaatacc ttatatattg gcagcctcaa
    25021 aagcgaggtt tatttttgtt tgtacgaaaa agattatgaa caatatatca agttagggat
    25081 tccattggac cagaccgaaa cgaaaaatcg ttttgagatt cggttgaaaa atgaccgtgc
    25141 ttattatgcg attcaagatt tgttaaaagg tcgtagcatt gaaagtacca cgttttccat
    25201 tatcaatcgg tatttgcgat ttgcggataa agtagatgga aaacggcgaa ccaattggcc
    25261 cttgaatgaa caatggggtc gttttattgg acggaatcgg aaagagattc aattaacttc
    25321 tgaaccgaaa ccgtatacca ttgaacgaac attaaattgg ctaggtagac aagtcgcccc
    25381 tacgtggaaa atggctaagg agttggatcg attgaatcaa acaacgtata tccaggatat
    25441 ggtacagaat gcgcgactct cggaccggca taaaaagatt ttggagcaac aaagtatggc
    25501 aatcgaaaac ttgattgtat gaaaagagga attttgatgg aaattgtaaa agatatgtta
    25561 ttggtacttg gtggcagtat gcttggtgtt accgtgatgt gcttgatgaa tgcaagtgca
    25621 gcagcggatt gagcaatgga aaagaaagga aagtgacaaa atgaatttcg gacaaaattt
//
LOCUS       DAZUMT010000031        24996 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000031 DAZUMT010000000
VERSION     DAZUMT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.031"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..763)
                     /locus_tag="ACG8FM_001169"
     CDS             complement(152..763)
                     /locus_tag="ACG8FM_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5093844.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(886..1011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1093..1809)
                     /locus_tag="ACG8FM_001170"
     CDS             complement(1093..1809)
                     /locus_tag="ACG8FM_001170"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5093845.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1815..2384)
                     /gene="scpB"
                     /locus_tag="ACG8FM_001171"
     CDS             complement(1815..2384)
                     /gene="scpB"
                     /locus_tag="ACG8FM_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS5093846.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2368..3138)
                     /locus_tag="ACG8FM_001172"
     CDS             complement(2368..3138)
                     /locus_tag="ACG8FM_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS5093847.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3135..3506)
                     /locus_tag="ACG8FM_001173"
     CDS             complement(3135..3506)
                     /locus_tag="ACG8FM_001173"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5093848.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3525..4412)
                     /gene="xerD"
                     /locus_tag="ACG8FM_001174"
     CDS             complement(3525..4412)
                     /gene="xerD"
                     /locus_tag="ACG8FM_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS5093849.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4508..4981)
                     /locus_tag="ACG8FM_001175"
     CDS             complement(4508..4981)
                     /locus_tag="ACG8FM_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5093850.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5086..5949)
                     /locus_tag="ACG8FM_001176"
     CDS             complement(5086..5949)
                     /locus_tag="ACG8FM_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS5093851.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6145..8244)
                     /locus_tag="ACG8FM_001177"
     CDS             complement(6145..8244)
                     /locus_tag="ACG8FM_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS5093852.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8464..9573)
                     /gene="rpoD"
                     /locus_tag="ACG8FM_001178"
     CDS             complement(8464..9573)
                     /gene="rpoD"
                     /locus_tag="ACG8FM_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS5093853.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9597..11438)
                     /gene="dnaG"
                     /locus_tag="ACG8FM_001179"
     CDS             complement(9597..11438)
                     /gene="dnaG"
                     /locus_tag="ACG8FM_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS5093854.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11620..13935)
                     /locus_tag="ACG8FM_001180"
     CDS             complement(11620..13935)
                     /locus_tag="ACG8FM_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5093855.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14120..14980)
                     /locus_tag="ACG8FM_001181"
     CDS             complement(14120..14980)
                     /locus_tag="ACG8FM_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS5093856.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(15039..15671)
                     /locus_tag="ACG8FM_001182"
                     /pseudo
     CDS             complement(15039..15671)
                     /locus_tag="ACG8FM_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            15838..16599
                     /locus_tag="ACG8FM_001183"
     CDS             15838..16599
                     /locus_tag="ACG8FM_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5093857.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16625..19216
                     /gene="mprF"
                     /locus_tag="ACG8FM_001184"
     CDS             16625..19216
                     /gene="mprF"
                     /locus_tag="ACG8FM_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5093858.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19404..20591)
                     /gene="tuf"
                     /locus_tag="ACG8FM_001185"
     CDS             complement(19404..20591)
                     /gene="tuf"
                     /locus_tag="ACG8FM_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5093859.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20959..21315
                     /locus_tag="ACG8FM_001186"
     CDS             20959..21315
                     /locus_tag="ACG8FM_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5093860.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21556..22560)
                     /locus_tag="ACG8FM_001187"
     CDS             complement(21556..22560)
                     /locus_tag="ACG8FM_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS5093861.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22726..23577
                     /locus_tag="ACG8FM_001188"
     CDS             22726..23577
                     /locus_tag="ACG8FM_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5093862.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23594..23839
                     /locus_tag="ACG8FM_001189"
     CDS             23594..23839
                     /locus_tag="ACG8FM_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093863.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            24026..24730
                     /locus_tag="ACG8FM_001190"
     CDS             24026..24730
                     /locus_tag="ACG8FM_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093864.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7044 a         5177 c         4363 g         8412 t
ORIGIN      
        1 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
       61 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
      121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
      181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
      241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
      301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
      361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
      421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
      481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
      541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
      601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
      661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
      721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
      781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
      841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
      901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
      961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
     1021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
     1081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
     1141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
     1201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
     1261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
     1321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
     1381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
     1441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
     1501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
     1561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
     1621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
     1681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
     1741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
     1801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
     1861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
     1921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
     1981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
     2041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
     2101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
     2161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
     2221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
     2281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
     2341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
     2401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
     2461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
     2521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
     2581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
     2641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
     2701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
     2761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
     2821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
     2881 gtctgtgacg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
     2941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
     3001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
     3061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
     3121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
     3181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
     3241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
     3301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
     3361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
     3421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
     3481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
     3541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
     3601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
     3661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
     3721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
     3781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
     3841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
     3901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
     3961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
     4021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
     4081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
     4141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
     4201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
     4261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
     4321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
     4381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
     4441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
     4501 tagacactca gacggactgg tttctttttt ctttttcata gcattcctga cagattccat
     4561 ggaacgttag ccgatgatct tttactaaaa aatgataacg ttcttctact acttgctcca
     4621 cttcaagtaa aagatcctct tctacttcct cgatcgtgcc gcagttcaag catagtaaat
     4681 gatgatggaa atgtttggcg ccttcttttc tcaaatcata acgggctaat ccatcattaa
     4741 aacttacttt atcgacaatt ttcaattctg tcaagatttc aagggttcga taaacagtag
     4801 caagtccaat ttccggactc ttttttttca ctaaaaagaa gatttcttct gcagataaat
     4861 gctctttctc attttccaaa agcacaagca gagtagcttc tctttgcggt gtcagcttaa
     4921 accccgcttc atgtagctgc tgttttgttt ttttcatcgc cgaaatagca ttcatgacca
     4981 tttactcaac cccaacaatt tataataatt acaaataaaa aaagatataa cactatgtta
     5041 attaaaatta ttataagcta gttaagaaaa acatgcaagg gattctcaat tagatttttt
     5101 gactagtttt gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa
     5161 tgcgcgctta aatgctcctt tgctgatacc aaaagctttc atgatttctt ctggatccga
     5221 tttatctgta aagttgattt gctgatctgc agaacgttct aaaatggtta aaatcatttg
     5281 tgcatcatct gatatagctt catatgctct tggcttcaat gaaagattca gtacaccgtc
     5341 tggtcggaca ccaatcactc gaccttttat ctgctcgcct agacgaggtt cattatatcg
     5401 ttcagaagga tggataaacc caagatagaa atcttccgtt aaaacgtacg tacctgttaa
     5461 tttcaagcgg tatgctgttc ctatcacatc tttgtttttc atttcttctg ttccatattt
     5521 gcttaaagat ttgaatattt tttcatctgc taaactgccc caaatacggt cttttgcatc
     5581 aactctaagg gaaatcatca agcgatcccc ttttttaggc catagctctg tcattgtcgg
     5641 caattcatca agagatacga caaagtcttt atctggaaga ccgatatcga caaacgcacc
     5701 taaatctttt cgtgtagcag taacgattcc aaaagcgtaa tgaccgatac ggcttttagg
     5761 aatttgtgta gtgatccgat tttcatgttt ttgattttgg taaccgaatc cttcaactgc
     5821 ttcaccgatt ttgtgttcac cttctgattt gcttaagcca aaggtttgtc cgtttttttg
     5881 gacaaagtag tttttttcat tttcatcaat aataatacct gtaaatatag ttgctaataa
     5941 atcgttcatt tttttctccc tctatagtga aaggttttct taatacattt ctttgctatc
     6001 ataacataat gttatctact tgaatagctg agccttggtt tggaaaggtg aaaaaaggag
     6061 tatgacaaca gttgtgtcat actccccttt ttcgaataaa cggtgttaaa cggcaggctc
     6121 acagcctctt tcatttgcct aaaattaatt gctcccattt cctccgaacc atccagtttt
     6181 acagttgatg gttacgatgt aaaatggcaa agcactttct ccaatgcctt tcatcaatgc
     6241 agattttgtt gaagtggcat atagattata atagccttcc ccattttcga ttctggcttt
     6301 ttctaatctg taacctcctt caacgatata tccacgttgg atacatgtag caatcacttt
     6361 gctttggaca cctggtgccc acgtccatgc tggattagat gcatcggcaa tatcacttgc
     6421 tctggcagat ttgattggct gattcgctga gtttgaagag gaaggggtcg aggatgccgc
     6481 ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc
     6541 ttgagcggct tgttttgctt gttcttcagc tgcttttttc gcttcttctg cttttttctt
     6601 ttcctcttct tcttttttct ctttatcagt gagcatctta tctacttgtt tcaattgttc
     6661 attcaactga gcagatagat ctgtattttt cactttttct actgcttctt ttgccgtctt
     6721 gtattggtcg cgtgttgctg aatccactac ttttccgtct ttaaagacta tagctgtttt
     6781 ttctttagct gtttgcaatt cagcatattg atttttagct tctgtttgtg cttgttgaat
     6841 caattttcca aaatctgtgt tatccgtttc gattgcttgg atctctttat ctgctttcaa
     6901 cttgatgtca tctgctagat gatcatcgac gatggcaggt gctatgaacc actcattcac
     6961 tttttggata tctcggatct tagattggat atcttggtaa gttgtttcta actctgaata
     7021 tcctttttca ttcttaagac tggataaatc attttttagt tttgataaat cttgatgaat
     7081 catatctgca cgaataaatt gatggtgatc atctgtataa aaggcagcaa gtttctcatg
     7141 aagttcttcc atttgttgct gtctttttgc ttctgctgct acttcggctt ctactttttg
     7201 atgatggtca taataagccc agcttcctcc accgagtaga agtactgctg ctaaaacgag
     7261 ataaggtact ttttttgatt ttttcttttg cgctttatct ggatctgttt tttctaagtt
     7321 tgattcttga tgtgtttttt cagtatctgg cacgattgtt attgatttcg cttctgcttc
     7381 aaacagctta ggctggttct cttctttttc tatttttgct tcaagatcag taccctcttt
     7441 gcttacagat tgattttcag catcggattc actatttatt tcatcgggct ctgttgactt
     7501 tttgtctgtg ttttcgagta tttctttttt ctctgcttcc ggcagtttat tcgttttttc
     7561 cttttctaat tgtttttcag taaatacttc agttttttct gtgatatccg tttttacgtc
     7621 ttttattttt actgtttctt tacctgtggc gttttctcta tcagagtctt tttcagtttc
     7681 ttgttccttt tctggctcta ctttgttttc ctgtgcaatt ttttcactga tcattttgag
     7741 cagctctttc gcgtgctctt cttttgtatc ttcacgatgt gctttgatat aagcggatag
     7801 tatcgaattt tcttcctcat tatcgacgat ttctttcagt tcagctactc ttcttttttg
     7861 ttttgattca agcgtttctt cttgattctt tcctgattct tccgaagtct catgaacagt
     7921 tgctttcttc tgttcatttt tctctatatt agttacttga tcgtgagcag gtggttcttc
     7981 gtcttctggt tctgcttttt cgaataattc cattacagat tctagaggta aatctttcaa
     8041 ttccgaccat tcgattggat cgtttaattc tgtatcagga aagactgcat cttcagttga
     8101 tttatcctgc atagctgcat ctgtatttgt ttcttcaatt gattctttta tttgctgatc
     8161 ttctgcagtt atttgatgac cgcagtctgg acagatagta ttctgctcca tttttttcga
     8221 tccgcaattt ggacattttt tcatcactta gcgatcggct cctttactag gtttacgcta
     8281 ccatttaatc tatcatattc tatacgtcac gccaatagct tatttttagg atttacttaa
     8341 aaataatgaa tgaatttcct tcctatagaa aaaaaagctt gagactgcgt agtctcaagc
     8401 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
     8461 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
     8521 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
     8581 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
     8641 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
     8701 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
     8761 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
     8821 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
     8881 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
     8941 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
     9001 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
     9061 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
     9121 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
     9181 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
     9241 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
     9301 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
     9361 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
     9421 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
     9481 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
     9541 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
     9601 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
     9661 tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
     9721 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
     9781 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
     9841 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
     9901 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
     9961 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
    10021 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
    10081 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
    10141 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
    10201 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
    10261 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
    10321 cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag atattcatca
    10381 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
    10441 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
    10501 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
    10561 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
    10621 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
    10681 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
    10741 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
    10801 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
    10861 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
    10921 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
    10981 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
    11041 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
    11101 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
    11161 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
    11221 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
    11281 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
    11341 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
    11401 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
    11461 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
    11521 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
    11581 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
    11641 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
    11701 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
    11761 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
    11821 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
    11881 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
    11941 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
    12001 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
    12061 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
    12121 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
    12181 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
    12241 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
    12301 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
    12361 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
    12421 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
    12481 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
    12541 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
    12601 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
    12661 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
    12721 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
    12781 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
    12841 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
    12901 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
    12961 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
    13021 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
    13081 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
    13141 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
    13201 caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc
    13261 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
    13321 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
    13381 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
    13441 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
    13501 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
    13561 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
    13621 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
    13681 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
    13741 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
    13801 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
    13861 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
    13921 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
    13981 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
    14041 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
    14101 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
    14161 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
    14221 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
    14281 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
    14341 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
    14401 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
    14461 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
    14521 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
    14581 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
    14641 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
    14701 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
    14761 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
    14821 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
    14881 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
    14941 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgta ctccttcttt
    15001 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
    15061 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
    15121 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
    15181 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
    15241 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
    15301 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
    15361 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
    15421 tgcagacttt ccgagctctg catgagatct gtcccttttc cttttgattc ggtcaatatt
    15481 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
    15541 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctca taaaaacaac
    15601 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
    15661 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
    15721 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
    15781 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
    15841 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
    15901 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
    15961 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
    16021 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
    16081 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
    16141 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
    16201 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
    16261 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
    16321 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
    16381 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
    16441 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
    16501 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
    16561 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
    16621 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
    16681 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
    16741 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
    16801 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
    16861 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
    16921 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
    16981 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
    17041 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
    17101 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
    17161 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
    17221 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
    17281 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
    17341 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
    17401 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
    17461 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
    17521 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
    17581 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
    17641 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
    17701 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
    17761 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
    17821 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
    17881 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
    17941 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
    18001 cgttattggg gtatacaatc tgccggattt ccctcatcgg cgtcaccatt ttatttcctt
    18061 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
    18121 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
    18181 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
    18241 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
    18301 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
    18361 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    18421 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    18481 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    18541 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    18601 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    18661 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    18721 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    18781 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    18841 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    18901 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    18961 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    19021 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    19081 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    19141 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    19201 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    19261 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    19321 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    19381 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    19441 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    19501 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    19561 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    19621 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    19681 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    19741 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    19801 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    19861 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    19921 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    19981 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    20041 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    20101 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    20161 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    20221 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    20281 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    20341 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    20401 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    20461 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
    20521 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
    20581 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
    20641 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
    20701 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
    20761 cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa
    20821 aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt
    20881 taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata
    20941 aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt
    21001 ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa
    21061 atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact
    21121 tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc
    21181 gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt
    21241 aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa
    21301 aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaaagagg ctgggacaga
    21361 agcgtttaac tccaagaaat aagaaggagt tgcttctgct tccgccgttt atacgtgttt
    21421 agagcgtgag acaaaagtgt tctttacttt tgtctcacgc tctttttttg ttatattcgt
    21481 cttttccgat ccttctcttg acacaattca tttaactgat cactggtact tgaacaccta
    21541 atattctagt catttttata aacgattcaa tacttcatcc acatttttga tcatcacgga
    21601 tatcgtacca tccggattat tcacaaattc aatcaaatct gggttgcgat atacatctac
    21661 tggtacgatc agttcgatcc cgtttgatag cttgaatttt tgttttccaa attttttctc
    21721 agaaatttct tgtacttctt ttacaggagg tgcttctggg acaaatcctt gctcttgtac
    21781 ttcttcttta aaagccattt ttgccgtgat gttttccttg aagacttttt ccgcaatcat
    21841 ctcatgatcc aaccgacctt tttcttctat cgtatcatag acagcttctt tcaaatcagc
    21901 catgatatca aattcttctg tctcaaattt ttttccaaga tgcttggcta ctttttggat
    21961 cacttttaca ttttcttcta aagaaggtgc aggaacactt tcgatcactc ttccagaaaa
    22021 ataaagctct tttttgccag aaaactcata ttttttttca attaattcgt aggtccaatc
    22081 agtcaaattc acagcgaatg cttcatctgc tttttgtgtt ttcgccccta gaattgctcg
    22141 attcaaaatc aagcggttgt cgatccctgc ttcgtctgct tcaacaaagt gagtgtaagc
    22201 ctcacgataa ttgactttca ataaagataa gtacatcact gtatctaatt catacaagac
    22261 aacaaataca tctgcactag gtgcttcttc actttcttgg tagatatcga accaccgctg
    22321 aacgattgct tcacttgctt ccacaaaatt ttcttgagcg attttaagtg attcaacgat
    22381 catgctttct tctgctagtt gtccagtttt tgtttgagca gaagagattt tttggatctt
    22441 cttttgaaga aaatcacgaa tatattcttt tgtcagatcc agctcttttt gtgaaaagac
    22501 tggagaacca gtttctctgt caataatatg cagaattgct ttttttaaat agatatccat
    22561 tttctcattc ccttctgtct tagtttacgg aaaaaaaatc aaaaatacca gaatcaaaat
    22621 cattttcttt taaacaaaaa tttcaagagt agaaacaaaa tggattcttt ttttcgaaaa
    22681 atgcgctaca cttatagttg aacgaataaa aaaggagctg aaagtatgaa ttcattaaca
    22741 agcacttatc gtctatcaaa tggttacgaa atcccggtag taggattcgg cacatggcaa
    22801 acacctgatg gggatgtcgc tgtttcctct gttaaagaag cgctggctgc aggttatcgc
    22861 catatcgata ctgcccaagg atacaagaat gaagaaagcg tcggccaagc gattaaggaa
    22921 agtggaattc ctagagaaga gatattcttg acaacaaaat tatggaatgc aaaccacagc
    22981 tatgaacttg tgatgtcttc ttttgaagaa tcattgaaga agctccaaac agattattta
    23041 gatctgtttt tgatccactg gccaaaccct gttgcttttc gtgataattg ggaacaagca
    23101 aatgcagata catggcgagc atttgaagaa ctgtatgaag caggtaaaat caaagcaatc
    23161 ggtgtgagca atttcttacc ccaccatcta gatacactag caaaaacagc aaaaattatg
    23221 ccgatggtca atcaagtttt ccttgcacca ggcgaattac agacaactgt agttgagtat
    23281 gcaaaaaaac atgatatgat cctcgaagca tatagtccat taggcactgg taaaatcttc
    23341 gatgtgcctg aaatgaaaca aatcgctgaa gcacatgaca agacgattgc acaagttgct
    23401 ttgcgttggt cgctgcaaca cgaattcctt cctcttccta agtcagtgac accaagcaga
    23461 atcaaagaaa atactgaatt atttgatttt gaattaacag aagaagaaat gaaacaaatc
    23521 gatcagttag atggtgtagt cggcaaagca aaagacccag atactaccca attttaagtc
    23581 gaaaggtgat tttatggatc caatcgaacg cttgaattct ctctcggaag aagttaccca
    23641 gacttttcat tccgattttg tctttttgat cgatgcagag aaaatccaac attttcctgc
    23701 aagaaactgg acacatgatc aaatcatcga agaactgaaa aaacggttcg atcattcgct
    23761 aatggtcaaa ccctggcacg aacatgaagt catttattca cctgaactac ctgtttttgc
    23821 cttgataccg aaaaaataga aagaatggaa cggattatgt atatatacat aatccgttcc
    23881 attttttatt tttctttcaa aaaacgggaa aatacgcaaa tttttctgtt gtaatttgcg
    23941 tatacgcaag tttttttgca ttactccttt tcatttggct gtttttttct tataatcgaa
    24001 agggtatgag cgaaggagag aacatatgaa gaaaaaacag aaacaaaaat tattaaatca
    24061 attccgtcct tcattggatg gtgtaagagg acaattattc cgttctctag aagaagagag
    24121 cttgcgacgt tatggtttgc cgttacaagt catgcttgat cccaaaaacc gtcaaatctt
    24181 aacgatcgga ttagctggga aaacaaatga agatacacgg atcaacgtgc caatcgatga
    24241 caacttcaat actgtattaa agagaattcg ttcaggagaa aacgggattt tcgaacgatt
    24301 ccgtgacaat cttcttattg aaatcgtgtc ttactggaat gatcaacgat tgaaaaacga
    24361 tgaaccaact gcgcaagctg ttgcgacacc agttacagaa gaaacagtca gtgtgccaga
    24421 agaaaaaacg gaaaaaccaa aagcggacac aactgaaaat acacgctcaa ctgatacgaa
    24481 tgccgcttta agttttgctg atttttcaaa agccgttgct gagtatccta aattttacac
    24541 agaacaaaat gaacatgaag tagttattaa tgaaaaaaat gctgatgaac ctcgtaaatt
    24601 agcaactatt tctatgacta ctgaaaatga gttcgttatc gaaaaagcac tcgaacggaa
    24661 atacaaagtc aaattgactt tgattccttt gatcgaacaa tttgcagcga cagcgattac
    24721 tgatcgataa aaattctatt tgtcataaaa ggctgggatt taaaatccca gcctttttag
    24781 tcatataata atttctacag caagtaaaaa gaaattcctt ctaacatcag gctcaaatat
    24841 cagcttgacc tgtccaacaa atcacctatg atcctacaat gttaggcgac ttctcttcta
    24901 cctgaagata tcttggagtt gcctgatttc ttaagatgcc taaccttaac tataaaaaat
    24961 tctaataata aacatatttt ctgatagaga aaaaag
//
LOCUS       DAZUMT010000032        24531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000032 DAZUMT010000000
VERSION     DAZUMT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.032"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..248
                     /locus_tag="ACG8FM_001191"
     CDS             102..248
                     /locus_tag="ACG8FM_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFS5093865.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            324..1571
                     /locus_tag="ACG8FM_001192"
     CDS             324..1571
                     /locus_tag="ACG8FM_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5093866.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            1586..2338
                     /locus_tag="ACG8FM_001193"
     CDS             1586..2338
                     /locus_tag="ACG8FM_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5093867.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(3041..3256)
                     /locus_tag="ACG8FM_001194"
     CDS             complement(3041..3256)
                     /locus_tag="ACG8FM_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS5093868.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(3275..3511)
                     /locus_tag="ACG8FM_001195"
     CDS             complement(3275..3511)
                     /locus_tag="ACG8FM_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS5093869.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3769..5922
                     /locus_tag="ACG8FM_001196"
     CDS             3769..5922
                     /locus_tag="ACG8FM_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS5093870.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5891..7306
                     /locus_tag="ACG8FM_001197"
     CDS             5891..7306
                     /locus_tag="ACG8FM_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS5093871.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            7339..8373
                     /gene="sppA"
                     /locus_tag="ACG8FM_001198"
     CDS             7339..8373
                     /gene="sppA"
                     /locus_tag="ACG8FM_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5093872.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            8375..8896
                     /locus_tag="ACG8FM_001199"
     CDS             8375..8896
                     /locus_tag="ACG8FM_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5093873.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            8893..9279
                     /locus_tag="ACG8FM_001200"
     CDS             8893..9279
                     /locus_tag="ACG8FM_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5093874.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(9775..9847)
                     /locus_tag="ACG8FM_001201"
     tRNA            complement(9775..9847)
                     /locus_tag="ACG8FM_001201"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9812..9814),aa:Lys,seq:ctt)
     gene            10228..10797
                     /locus_tag="ACG8FM_001202"
     CDS             10228..10797
                     /locus_tag="ACG8FM_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS5093875.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            10784..11644
                     /locus_tag="ACG8FM_001203"
     CDS             10784..11644
                     /locus_tag="ACG8FM_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5093876.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            11834..12538
                     /gene="yycF"
                     /locus_tag="ACG8FM_001204"
     CDS             11834..12538
                     /gene="yycF"
                     /locus_tag="ACG8FM_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS5093877.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            12543..14378
                     /gene="walK"
                     /locus_tag="ACG8FM_001205"
     CDS             12543..14378
                     /gene="walK"
                     /locus_tag="ACG8FM_001205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS5093878.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            14375..15691
                     /gene="yycH"
                     /locus_tag="ACG8FM_001206"
     CDS             14375..15691
                     /gene="yycH"
                     /locus_tag="ACG8FM_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS5093879.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            15692..16561
                     /locus_tag="ACG8FM_001207"
     CDS             15692..16561
                     /locus_tag="ACG8FM_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS5093880.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            16616..17425
                     /locus_tag="ACG8FM_001208"
     CDS             16616..17425
                     /locus_tag="ACG8FM_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5093881.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(17528..18157)
                     /locus_tag="ACG8FM_001209"
     CDS             complement(17528..18157)
                     /locus_tag="ACG8FM_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5093882.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            18327..19619
                     /locus_tag="ACG8FM_001210"
     CDS             18327..19619
                     /locus_tag="ACG8FM_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5093883.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(19695..20357)
                     /locus_tag="ACG8FM_001211"
     CDS             complement(19695..20357)
                     /locus_tag="ACG8FM_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5093884.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            20582..20866
                     /gene="groES"
                     /locus_tag="ACG8FM_001212"
     CDS             20582..20866
                     /gene="groES"
                     /locus_tag="ACG8FM_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS5093885.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            20917..22542
                     /gene="groL"
                     /locus_tag="ACG8FM_001213"
     CDS             20917..22542
                     /gene="groL"
                     /locus_tag="ACG8FM_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS5093886.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(22631..23770)
                     /locus_tag="ACG8FM_001214"
     CDS             complement(22631..23770)
                     /locus_tag="ACG8FM_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5093887.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYGEGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLSNRAKELIESVIA
                     DNILAGRPTNPDQYIFISTNGTPLTLHSFNAVLHKVEEELELEKNLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKKAKQQVIDKLNRL"
     gene            complement(23881..24465)
                     /locus_tag="ACG8FM_001215"
     CDS             complement(23881..24465)
                     /locus_tag="ACG8FM_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS5093888.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
BASE COUNT         8265 a         3913 c         4805 g         7548 t
ORIGIN      
        1 atacttataa tattaaatat gtctcaaaat tttttgatta gaaaaatagg tatattgaat
       61 cagtgacata aaagtattta tagtttgtag ggggaatcgt aatggaagaa aaaaatagac
      121 taaatgctaa acaatgttca gatcaagagc ttaaaaaaat taaaggaggt gctggaacaa
      181 aacctcaagg aaaaccggca tcaaatttag tggaatgtgt tttttcatta tttaaaaagt
      241 gtaactaaaa ttttttttat gatatcctaa aatcaggagg ggatctcttg attactatcg
      301 atacaaaagc ttatctatta agaatgttcg tgggaaactt tttctttttt ctcttatttt
      361 atatgactaa tactgatctt aacaaaagaa aatatctttt tgtatgttta ttgataccac
      421 tcactttttt tgttgaatta tttactgatg tttccgcagc tatacctata atcggaagtt
      481 attttatatt aaaggaaaag aagaaaacta atatagtgct tttaaataaa ttactattat
      541 gtatgcttat tgatgatttc agatctatta ttacttccat ggttatgacg tatatgtttt
      601 catttgaagg gataaaaggg tatggttatg tagtattcca gataatgctt gattctttgt
      661 ttttgctgat gtttatttgg ttatatagaa aattagctat aaataggatt gttgaacagt
      721 ccagttctaa acttactgct atattcatga tttatttact tacaattggt ttatttgttt
      781 cttatgttgc tcatcaatat caagtatttg atcattttgt gtttggtgtc ttaatatttt
      841 taatcattca aatgtttgtt gttctatttt tatttattag aataactatt aggcaacgag
      901 aacaatatga acagcaattg aaaagaaaag aattggttta tctaaaaaaa tatactgatt
      961 cactggaaaa agatcaagaa aaatttgcca gatttagaca tgactataaa aatttattgc
     1021 tgagtttaaa agaaatcgct aatcaaaacc atgattcgat attggaaaaa gaaataaaag
     1081 aattagagat ctattctaat tcttatctaa ccaataagtt tgaatatcgt tatttaaaaa
     1141 atataaaaaa tgaatatttg aaaagtttgt tgatagcgaa gctataccaa gcaaataaca
     1201 ataagatttg ttgtaaattc gaatgtccaa gtactattat tgaagttcct gttccaatat
     1261 ttgactgtat tagggtgctt ggaattattc tcgataatgc aattgaagca tctatagaga
     1321 gtcaagatag agaactgtct ctagctattt accgagatga taaacagata gaaatttcga
     1381 tatcgaatag ttgtagagag atactactct ctatagatac tctaatgaaa aaaggaataa
     1441 caacaaaaaa gggacatcaa gggttaggtt tatctagtat agaggaaatc aataaaaaga
     1501 atcccaatat gtttgtgaac tatcaaaacg atccgtatca atttactact caagttattt
     1561 tgttgttttg agggaaggag gatttatgag ttatccaata atattatgtg aagatcaaat
     1621 tatacaatta aatcagttag aacgaattat tgataatttc attctttttc acgataaagt
     1681 atttaagata gtattgaaaa cacaaagtcc gttagaagtt aaaaaatacc tcaaacaatt
     1741 ccgaccaaag caaggaatat atttcttaga tattgattta aatcatgaag ttaacggtat
     1801 agaattagca gaagtaatca gaaaatatga tgttcaagca aaaatcattt ttacaactac
     1861 tcatgatgag atgttacccg taacaataaa aagaagagtt gaaacgttag gatttgtaac
     1921 aaaagatcaa acactagatg agtatcgaaa cgagattgtt gagttattgt tattagcgca
     1981 agaaaggata gatgcaacaa aacaggttac gaatcaagca tttgtttttt ccataggttc
     2041 tcaaacattt acttttgata taaatgacat ttattttgta gaagcttcaa aattaccaca
     2101 tagattatca ttgtgtacta aagatgggca gtacgaattt tatggaagaa ttagtgagct
     2161 tgaaaagaaa tatcctatgt taacaagaat tagtcgagcg tgtttagtaa atatctttaa
     2221 cgttaaagaa atagatttta aaaaacgttc actatatttt gattcagagt tggccagaaa
     2281 ctttacttta gggaaagcac aaaaaattaa ggagaagttg aaagaaagca ctgtttagtg
     2341 gttttttctt tagggagatt gcatttttct aataaaatag attccttcac tagtgcaaat
     2401 ttttctgaat cctattaaag ttgtaccaat ttttatagct gttattagtt acgtgcataa
     2461 cagcaatatt agttactgga ggaatatttc tatggtctgg aaccaaaaaa gggccaaatg
     2521 aatagttctt ttcttgtttt tattaagtac cataaagggg aattaaaatt aaatgtatgg
     2581 tggaagatgc gaaagatttg gcaaattcta ctaattttta gaaagtgttt ttcatttttt
     2641 agttaaaaaa tttgtcctgt caagtgcaat cctatgattg tacttgatag gatttgagat
     2701 aattactttc ctgtttttat aaatttaact tgcaggaaca ggctttaatg cacatccgac
     2761 aagtacatta ccaatacctc ctgtaacggc cgaaataggt cctcctgcag caccaactac
     2821 agcgctgcaa gtccttttgt aaaaatatct ctaccattac atttccaaag aatttttatc
     2881 tgactttaat aaaagataaa aatttaatat aattataaaa actagcacaa gaagctttag
     2941 taaaaagttc tgtccattcc aaacttttga aaagataaag aacataccta tgttcgtaat
     3001 cgacacaaac caatcttgag gtttgatatt ttttaattgt ttacgcaccg gtccacatgc
     3061 tgaatccagc acctaataaa gctccaccta caccacaagc agctattgca taaggtcctc
     3121 cggctgaaca aagttttact cctgctgttg ccccagaaaa agtagtgcct aaaactccca
     3181 cggcactccc accttgaata tttttcaact cttctgaaga taatataaat gttgtttttg
     3241 tgtttttaat atgcatacta cttattctcc ttttttattt ttttcgagca tcccagccag
     3301 ctgcagcacc tccagcgaca gctccatata accctccaac aacaccgcaa gctggtccgc
     3361 aaattgctgt tcctaaacct gcaccaccta ctgcccctgt ggtaagtcca cctacaactt
     3421 gccccaattt tccccaagca ttattgcctc caccaacagt ttgtgcattt ccacaatact
     3481 tagttccttt acattttgca tgtttaaaca cgcttcttta aataatattt tttacggtgc
     3541 tactgtaaca tacgtatttg attaacagta aaaatcttcc tgaaacttat caagtttatt
     3601 cttgaatagt aaaatttcca aaataaattt gattcaggaa tgaaaaagtt agtgttcaag
     3661 aactgttttc aaagtttttg tggaattatg agacaatatt ttcactgtat taaagctata
     3721 ttgagaattt ttctataaaa attttttaga aggagttgtg ttagataagt gataaaatca
     3781 tttccttttg tgcagcaaca agatgaaaaa gattgtggtg ttgcttgcct ttcgatgatt
     3841 ttaaaatatt ttaaaactga tatcccgatt cataaattac gggacctatc tgggactgat
     3901 ttagagggta cttcagcatt tggattgaaa aagacatttg aaaaattgtc ttttgattgt
     3961 atggctattc aagcagatag tgagatctgg aaagaaagag atcttatctt accattaatt
     4021 gcacatatat tggttgataa cagctatatg cactacgttg taatttataa aacgaaggga
     4081 aacgtactat ggattgcaga tcccgctaat ggaaaaaata gaaaaacgat tgaagcgttt
     4141 caaaaagaat ggacaggtat tcttttatta cctatgccta gagaaactta tattcctatt
     4201 aaagaaaaag tagctggatt aagttctttt ttgcctataa tttggaaaca aaaaggatta
     4261 gtattccaca tcgttttagc atcgattttc attacgtttt ttggcatagg aagctcatat
     4321 tattttcaag gtattcttga ttttttcatc cctaatcaag cacgttcaac tttaaatata
     4381 atttccattg gcttgatcgc tgtctattta tttcgggtat tgtttgaata tagtcgtagt
     4441 tatcttctta ttattttagg acagcgaatg agtatggcaa ttatgcttca gtatttcaaa
     4501 catgtgttaa ctttaccaat gaattttttt gctacgagaa aatcagggga aattatttct
     4561 cgttttcttg atgccagtaa gattattgat gctttagcaa gtgctacgtt atcaatattt
     4621 ttagatattg gtatggtgtt gcttgttggg gttacgttag ctattcagaa tagtacgttg
     4681 tttttaatca ctctatgctc tttgcctttt tatgtagttg caatattcgc tttcgtgaaa
     4741 agctatgaaa aatcgaacag ggaagaaatg gcagccggtg cagctgtaaa ttctagtatc
     4801 atagaaagtc ttaaaggaat agaaacaatt aaagcatata gcggtgaaga gaaagtatat
     4861 aaccgtatag accatgaatt tgttaaatta atgaaaaaat catttcgtac ggttacgctt
     4921 gataatattc aacaaggaat taaacatttg attcaattaa ttagcagtgc attaatttta
     4981 tggctaggtt cttattttgt tatggataat agaattagtc taggtcaact tatcacatac
     5041 aatgctttac tagtattttt gacagatcct ttacaaaaca ttattaattt acaagtgaaa
     5101 atgcaaacgg ctcaagttgc aaataatcgt ttaaatgaaa ttttcgcgat tgattcagaa
     5161 aaatcgaacc agtcttttga atatatgctt tctgaacaat tatttcaaca agatatcact
     5221 gtaaaaaacg tttcattctc gtatagcatg aaggccccaa cgttgaacga aatttcatgt
     5281 acaattcctt cgcacagtaa aacggctata gttggagtta gtggttctgg aaaatcaact
     5341 cttgcaaagt tatttgttcg tttttatgaa gcatcagaag gatcgatcca ttatggaaac
     5401 attaatcact tagacattcc ttttcagata ttacgcgatc gagtgacata tttgcctcaa
     5461 gagtcatttt tctttagtgg aacaatcatt gagaatctta tattcggttt agatagccca
     5521 ccaagtttcg aacaaataat agaagtttgt gagatagtcc aactaaatga atttattaat
     5581 caacagccat tacgttacga gacattgttg gaagaaggtg gaaataatct ttcaggaggg
     5641 caacgacaac gattagcctt agcacgggct ttattaaaaa atccggacat attaatttta
     5701 gatgaagcaa ctagtggatt agataccttg ttagaacatg caattatgaa ttatctcctc
     5761 aatttaaaag aaaaaacgat tatttttatt gcgcatcatt taccaatagc aaaagcatgt
     5821 gatcaaattt tagttctcca tgaagggcat ttggtagaaa aaggaacgca cgatgaactt
     5881 cgttttgccg atggtatcta tcaaaaatta tgggaaatct aggagaggga aacaatttat
     5941 gcagaataat aattggttcg aacattctag tatctatact aaacaacatc atagcttcta
     6001 ccgttgggtg ttataccctg taattctttt tttatgtatt attggattgt ttttaacctt
     6061 tgctaaaaaa gaggttgtta ttcggacaac ggctcaattg aaccccgaaa aaatagagaa
     6121 gttacaagtg ccattagaag ctaaaataat ggaaaacttc ctaaaagaaa atcaatttgt
     6181 tcataaggac gatgtgttgg ttagattgga ttgttcttta atagagaatg aaaaggctca
     6241 gatagaacaa gaaaaccaac gtattactca acaaataaag atggctcagc tatttattga
     6301 aagtataagt aaaggaaaaa atttgttttc aacggatgac agttttggct acagtaatca
     6361 attaaagagc atgttgtcag aaaaagaatc actccgctac gctttgaagc aaagtgaatt
     6421 aaatgatcaa aagcaattag aagtatacga aaagacaaaa agacaactag aaaaacaaat
     6481 tgagagttca gatagtaaat tacaagaatg gcaacaagta caggtagctt ggagtaataa
     6541 tcaatcatta aaagattttt caaaagaaat gatggcaaac tatgagaatt ggcaagaaca
     6601 actaaataat gtttctgatg atcaaaaaaa tcaagtgaaa ctgacaattt cagcaagcat
     6661 aaatgaacaa attgagcaac taaaaaaaga agtagaacag tatcagtcag aaaaagctaa
     6721 attagttaaa ccaactactt ctgagaatga cagaattagt caaacggaaa aaggaaagca
     6781 agagctagaa caagcaataa caactgtaaa acaaacagtt acagagctaa atgaaaaaca
     6841 agagaagaac cagtcagtta tcaaaagttt ggacgatcaa ctttcaaaag gaatactaaa
     6901 agctcctgta tctggaacgg ttcatttaaa tgaagaaaca aagggacaag cagaactagc
     6961 caaaggaaca gtgcttgcag aaatttatcc gatccacgaa aaatcaaaaa tgaaatttac
     7021 agcactttta cctgcgaatg aaagtaccta tattaaagaa gggatgaagg ttcattttaa
     7081 attggatcaa aaaggtgggg ctccgattac gatcgacgga actttagatg aaatttcaga
     7141 aaattctact gccactgaga aaggtgtttt ttatgcagtg aaaggaacgt taaaaacaac
     7201 tcaatcgttt ccgtttcgtt atggattaac tggagagttg tctttaattg taggagaaaa
     7261 aacatattgg caacaaattg tcgatgtgtt tctaaataaa aaataattat ttttactaag
     7321 aatataggag gagtttatat gaataaaaga agatggattg ctgtgggagt agcgtgcgga
     7381 ttattgttac tatccattat cgtcgcgcta attccaggaa aagataaaga ggaagcatca
     7441 aatacaaccc taacaggaat aaataagatt ttttacggat ctaacgaaat cacagaagaa
     7501 acgttagaag aaggcgcatc aaataaaaag attgtcaaac tatccgtcaa tggagttatt
     7561 gcagatacag gtgaaagcaa tctttttagt agggaacaat ataaccatca gaatttttta
     7621 acacagctaa agaaaattca agaagacaaa gcggttaagg gagtgttatt agaagtaaat
     7681 tcacctgggg gaggaatata tgaaagtgct gagattgcta aagaaatggc aaaaattaaa
     7741 aagctagata ttcctatcta tactgcattt aaaaatacag cggcaagtgg agggtactac
     7801 atttctgctg gttcagataa aatatttgct acggaagaaa caacaactgg atcaattggt
     7861 gtcattattt ctggtttaaa ttattctggc ttattagaaa aacttggcgt aactgatgcg
     7921 acgtataaaa gtggtgcatt aaaagatatg atgtcacctc aacacaaacc ttcagaggaa
     7981 gagaataaag ttatacagga atttgtgatg agtgcttatg atcgttttgt caatgttgta
     8041 gcaaaaggaa gaaatatgga tactaatgca gtgaaagaac ttgcggatgg aagaatttat
     8101 gatggtaacc aagcagtaga aaatgggttg gtggatcaaa ttggttactc tgaagatgca
     8161 ttagattccc taaaaaaaga gaagaaatta actgatgcga cggttataga atataaaaat
     8221 gacacaactg gctttgcttc ttcttggtta ggtaataaga ttgctgaatg gcaagggcta
     8281 aaggcaacta catcagatcg tgtaatttca gtttttgaaa aacttggatt ttctgatact
     8341 ccaaaaccaa tgtattacta tggagggcta taaaatgaat aagaagagtg gcattgaaac
     8401 tttgcctaat tatgtatttg cgggattttg gttacgattt tttgcttttc ttattgattt
     8461 aatttgtatt agcatcatca cccatgctac tataggtctc ttttacaaaa tcatgggact
     8521 ggaatctgac aaatccatta ttacttttta tggtatagcg tcattagcta tttatttaac
     8581 ctattttgta cttttgacta agctgaattc aggacaaaca attgggaaaa tgatttttgg
     8641 gataagagta gtgagctttg aggaaaaaga gctaagttgg caaactatct ttattcgtga
     8701 gtgtatatgt aggtttatat taaaaaattt tgtatttcaa attgggtatt tggtggctgc
     8761 tttcacgccc aagaaacaac atattgggga ttatttttct aacacgtcag tggttgttat
     8821 tcgttcatta gaagctatta actatggtca aaaaatttct aattctaaaa cacttaatct
     8881 ggagagaatt aaatgaataa gagaaaaaca gagtttattt tgattttaat tagtggaata
     8941 acagggattt tatgtagtct tttagcattg atgggtggta tgtttctatc ttctattgat
     9001 tcaactactt taaatactac agaactaacc agtaaggata tagatatgtt agtcaatcta
     9061 agcgggaatg ttgttttatt tggaggaatt accctagttt tttcgataat gattttcata
     9121 ataggttttt tagtaagaaa aaatcaaaaa acagtggtct atggtattag tttactcatt
     9181 ttatctatta ttccattttt tcttttaaca ttattgtggg taattcctgg agttttaggt
     9241 attattgcgg gattgatgct tctaataaga aaatcttaat taggcaatgt gtagacaacg
     9301 tgaaacgatt aatcaattaa aagctaaata cgttttgaga aatgataact tgtagtcgat
     9361 ggaagacatg tagataaaaa aactacgtgt cttttattta ttctaatttt ttcgaaaaaa
     9421 agacactctc tgattgttgt attaatggaa aaggggaaag cagcaagtaa atttagtttt
     9481 tctattaaat attcttcact ttttaacctt ttatagtact tagatttaat cctatatgtt
     9541 tagagagttc attggcagca tgattttgca aagcttctcg aattattgtt atattagaaa
     9601 ctttactgtt cataattatg cttctttttt aactcaaagt ccaaagggct acagtggact
     9661 ttgagttaaa aataaaaagc cctagttatt ttgaaaaata aaaaaagaac agcgtaaatg
     9721 ttttcgaaat gctttcgcac caacatttac ctgttccgtg ggtttggcac ccaatgggcc
     9781 gtgaggggct cgaacccgcg acccgctgat taagagtcag ctgctctacc aactgagcta
     9841 acggcccgta caaaaagtac ttaataatag tagcactaag aattttaaaa tgcaagacta
     9901 catttaaaga aaatcagaaa aatctttact tccttatagg aaatcattca tagattattc
     9961 acatttgctc gctattatat aagcatacca acaaacaaag ttcttgcaag agaacaaacc
    10021 ctaacacttt tttcattttt tactcctttc ccgccgtctc cccggcgggt ctcctgttct
    10081 taacgataaa agacaagaag gcttagcctt cttgtttttt gttttcatgg aatcccgcta
    10141 atcaaatcac ctagttaaag caactagatt gaatgtttat cataagtctg atatcatgtt
    10201 tatgaaatga aaaaaggagc ggttatgatg gaaaaaatgc gtcagcaatt aatggagtgg
    10261 gggaaggaac tagaaacatt ccgtctgcct cactgggaag aactccctga cttagaactt
    10321 tatatggacc aagtcagaac gttagttgat cgttatcttt cacctgtgat acaaggagaa
    10381 aagcatccat tgcttacctc ttctatggta aacaattacg ttaaactagg tttgatacca
    10441 gcaccagtta aaaaaagata taacaaagaa cacgtagcat ttttacttgc aatcactacg
    10501 ttgaaacaag tattgactat tcctgaaatc aaagaaggga tattgttcca aggaaaaaca
    10561 gtaggaattc gtgaagccta taatctattt tgcgatgaac aagaagcagc tgtgtggatg
    10621 gtcagccaat tagcacaagg taaatctcat ccgcaaaaat tcgaccaggc aacccctgta
    10681 gaatatattg ctgtaagagc ggcaacttta tcgtttgcaa tgaaacttct tgcggaaaaa
    10741 acgattgttc tagaaaccga atatttaaaa gaggagaaaa caaatgaaaa acaataaaat
    10801 cgcaatttta gtagattcag gtacggatgt tccagaagaa ttgataaaaa aatacgagat
    10861 gtatatgatc ccattgaaaa ttatttataa agatcaggta tatacagata aaattgacat
    10921 tacccctgaa gagatctatc aacgtttgcc agaggaaatc cctggtacat ctttgccgga
    10981 tggtgagtcg atcacagaaa tctttgagca aatcaaaaaa gacggttatg aaaaggtact
    11041 tgcggtaaca atttccagtg gattgagcgg gacatataat gtggttcgtt taattgcaca
    11101 acagcaagaa gaattggaag catatgtact agatacgaaa aatattggta ttggtgcagg
    11161 tttttctgcg atccaagcag caaaatggct ggaagaagga gtagaatggg atcaactgat
    11221 cagtaatctg aatgagttag tcgaaagaac aaaagttttc tttaatgttg cgacattgga
    11281 atatcttcaa aaaggtggac ggatcggttt agttgcttct attgtaggaa ccgctttaaa
    11341 gcttaaccca atcatctcat gtaatgaaga agggatttat tacacagtag gaaaagctag
    11401 gggacgtaaa aaaagtttgg ataaaacggt tgcttacgtc aaagaaaaaa tcggtaatgc
    11461 aaaggtcttc aacttagctg ttgcccatgg ggatgcgaaa gaagaagcaa ttgaaatgga
    11521 agcgagactt aaaagagagt tcccacaagc aaaccaaatc tactttggcc agatttctcc
    11581 ggcattagta gtccacactg gtccgggatt attgggtgtc ggtgtacaag ttttagaagg
    11641 ataaatcaaa aaaaggaaac actgaggtga aaatcagtgt tttccttttt tattgaagtt
    11701 agaacaaaaa ataataaagt attttgcact ttcattttaa ttttctcttt tgttttatag
    11761 gcaaagatga aacaaaagca gaaatacggt ataatgagaa ggtatgacaa taatatataa
    11821 gaggtggctc tttatgaaaa aagttttagt tgtcgatgat gagaagccga tttcggatat
    11881 cgtcaaattc aatttagcca aagaaggcta tgacgtttat actgcatatg atggggaaga
    11941 agccttggaa aaagttgctg aggtagaacc tgatttgatt ttacttgatt tgatgctacc
    12001 taaaatggac ggcttagaag ttgctcgtga ggtacgcaaa acttatgata tgccgatcat
    12061 tatggtaaca gccaaagatt ctgaaattga taaagtccta ggcttagagt taggcgccga
    12121 tgattacgtg accaagccat tttccaaccg agaattagtt gcacgtgtca aagcaaatct
    12181 tcgtcgcggt gccacggctg cgaaggaacc agaagaggca gcacctgcgg aattgacgat
    12241 tggtgatttg acgatccatc cagaagctta catggtgacg aaacgaggag aaacaatcga
    12301 gttaacacat cgtgaattcg aactactctt ttacttggcg aagcaccttg gacaagtgat
    12361 gacaagagaa catttattac aaacggtttg gggttatgat tattttggag atgtacgaac
    12421 agtggatgta actgtacgtc gtttgcgtga aaagatcgaa gataatccaa gccatccaaa
    12481 ttatttagtg acacgtcgtg gtgtcggtta ctatttgcgt aacccagaac aggagtaatt
    12541 ggatgaaaag aaaagtccgc ttttttcgat cagtcaattt taagatcgct attaccttta
    12601 ttctgatttt attgatctcg atcgaaatta ttggtgccta ctttatacgt ggtttagaac
    12661 gttccacgat caatacattt attaaagata tgaatcaaac ggtggaatca ttagcaacaa
    12721 caatcagccc ggaattgaat cgaaaagata atgcagatga tgaagaggta aatgcgaata
    12781 tcaaaagatt tattgaaaac agtgcgacta gcgatatcat cgaaatacgg gtcgttgacg
    12841 aaaaagggat catccgagga acaaccgatg tcaatgaaca aagcgctgtg ggaaagaaaa
    12901 atgattatgt tgatatcaat gatttcacga ctaaacgtta tgttgcttta gataacgacg
    12961 ataaacgggt gaatatcaat gttcaaccga tcctgtcccc gaccggcgat gccgttatcg
    13021 gggctcttta cgtcaaaagt aatatcgaac aaaaatattc ggaaatcaat aacacagcag
    13081 tcattttctt tactgcctca ctaattgcgg catttatttc aatgatcgta tctgttttag
    13141 ttgctcgttc gattacacag ccgatcggtg aaatgagaga acaagccatt cgaatcgcta
    13201 gaggagacta cagtcggaaa gtcaaagtgc atggacaaga tgaacttgga cagctggctg
    13261 ataccttcaa tcagttagct gaacgtattg aagaagctca agatacaatg gaagctgaac
    13321 gcaatcgttt ggatagcgtg ctatcccata tgacagacgg cgtcattgcg acagatcgca
    13381 ggggtaaagt cattacgatc aatgatatgg ctgtttcctt gctagatatc aaaaacgagg
    13441 aagcaatcgg acaatcgatt ttgacacttt tagatatcga agaagaatat acgttgcgga
    13501 aattattaga agatccaaac gaaatggtac ttgaccgttc aaccagtaca ttggaaagtg
    13561 atcagatgat cttgcgagta gacttttcta tgatccgtcg cgaatcaggt tttatcagcg
    13621 gattagtagc tgtcttgcat gatgtgacag aacaagaaaa aacagaacgg gaacgtcgtg
    13681 aattcgtctc taatgtttcc catgaacttc gtacaccgtt gacaagtatg cgtagttata
    13741 tcgaagcatt gagcgaaggt gcgtggaaag acgaggaagt agcaccaaat tttttgaagg
    13801 taacgctaga tgaaacagac cgaatgattc gaatgatcaa tgacctctta aatctatctc
    13861 gcatggatac aggtaatacc cagcttcaat tagaatatgt caatttcaac gagttagtga
    13921 attttgttct tgaccggttt gatatgatgg tgggaaatca ggaaaaaaat tataagatcc
    13981 gtcgtgaatt tacgcaaaga gatctttggg tcgaagtaga tacagataaa atcatccaag
    14041 tagtcgataa tatcatgaat aacgcgatca agtattcacc agatggggga acgattactt
    14101 gtcgcttatt agaaacgcat aataacgtga ttttaagtat caccgatcaa ggattgggga
    14161 ttcctaaaaa agatctgaac cgagtgttcg agcggttcta tcgagtagat aaagcacgtg
    14221 caagagcaca aggtgggaca ggacttggat tagctatttc tagagaagtg attaaggcgc
    14281 atcgaggcgc tatttgggca gagagcaagg aaggaaaagg gtcgactttt tatatctcgc
    14341 tgccatatga accttatgag gaggaatggt gggaatgaga atctctgaaa aagtcgttcg
    14401 tataggactc attttgatgg ttgcgttaag catatacttc tcttatgcaa tctggctgag
    14461 cccagcagga aaaagttcaa tcaacgtaga tgagacaaac actcaaatga tcgagtctca
    14521 aagcaatcga aaaacttccg aagtctttct tccgcttcat gttacatggc accattcggg
    14581 aatgatccaa gaaaccaaca gtgaaaatct cgtgtaccga ttgcaattga tcatcgataa
    14641 agcaagattt ggaaagcttt ctgaagtagt ttcaggagac aaatcacagt ttgaacaggt
    14701 gaaaaatctg aactctggtt ttgagttggc ctataatgcg ccttttttgc taagagaata
    14761 caaggaagca tttgatttag atttagatgt gtcgaacttg gcaaaagaaa gcgaaaatat
    14821 gtattttact agaatacaat tcgatcaagg aaataaaaaa atccgatttt tgaattattc
    14881 gaaccatctc gtatatgaag caaatatttc gttgaattgg acagatatca ataaagaatt
    14941 aacagcaaaa gacacaaaat ggacagaaat gacaacagaa ccatcgacga tcgacacgca
    15001 atatgctacg aaacattcga tcaaactgaa gaagtatagt tatatcctgt cacagcaacc
    15061 ctatacactt tttagaaatg cttttttcca gaaaccagat agcgtgaaaa ataatgatga
    15121 aagcaatgaa ttgatttttt acggtgggaa tgaaacaatg tctatccatt cggaaacgca
    15181 agtagttgat tttcgtggag agctgccaga gaaaaaagaa aatgctagtg tattttctga
    15241 aagtttcccc tatattagca aattaggcag ttctttaggg aacctcagat attttgaccg
    15301 cagtgacaat acgatcgatt atcggatatt cgtggaaggc ttcccggttt ttgggacaga
    15361 cagcagagga cagattggtg tggaagttgg cggtgaaacg aatggttctg tgcaagtgaa
    15421 tatccaaacc agtttgaata cgattcaagt gccgatacct tcagacgaag aagtagaact
    15481 tgcaagtacg aatgaaatca tccagcagtt aaaagctaag ggagcagata gcaacaagat
    15541 tggttcagtg atcgttggct atacatggca aaatgtcaaa gaaacgaatc gtgtcgttga
    15601 tctcttacct gaatggtatg tgaaatataa agataactgg tattcagcta acgaccttct
    15661 ggaaaaatta cctggattgg aggcgaatta attggatttc aaaaaaatcg aatggatatt
    15721 tttcgtcgct tttttagggt tgaatgtgtt tttattcagc agttatcatg acgcggtata
    15781 ccaagaaaac aatgtcattc gctctaatca gactgaatca atccaacaac gtctggctag
    15841 cgacgatatc acttacacgg ggaagatttc gtctgagaac aagcaaggct attatcttag
    15901 cgcggagcag accaatctta gcacttcatt agcgaattat cgcaaggctg gtaaaagcgg
    15961 cttgctggca agtggtgtgt ttttagacgg tgatgttttg acgaaatctt tatccagttc
    16021 agatgctgaa aagaacgtga tcgatccaga taaaattagt agttccttgg aagatttttt
    16081 atctaacaaa gacaatattt tattcggtaa tgattatgtg tacacgaaaa acttttctca
    16141 tttagaagaa gaaccactag aaatccaagc aagccaagag tatgaaggca tcccattcaa
    16201 tgatgataca gcaaaaatcg ttctttcttt agaaaaaaac gataatgatt atagcatcac
    16261 gaaatacatg cagacgcatt tatctgatat cgagcagctg cgcgaaaaga cggaactgta
    16321 tacggaagag gaagcaatca atacattgta catcaataac aaaataccaa gaggatcaaa
    16381 aattttatgg cgtcagctag cttattcctg tattctgaaa gtccgggaaa aaaatgtgta
    16441 tgttcctgtt tggcatgtag cgatcgaaac aagcgataaa gtggtccaaa ttgagagcgt
    16501 caacgctttc agcaatacga tcgtgacaaa caatactgta ccaaaagtgg aagatcaata
    16561 aaaaagcgag tataatgaaa aaagtttaca aagtaagaag ggaagtagga gtcaaatggc
    16621 tgaagaaaaa ccagctttta aaatcagtat cctagcaagt ggaagttcgg gtaattcact
    16681 ttatatcgaa agtgaaaaaa aacggctttt ggtcgatgca ggacttagcg gtaaaaaaat
    16741 cacttccttg atggctcaaa ttgatcggac accggaccaa ttagatggaa tacttgtgac
    16801 gcatgaacat cgagatcata tacatggcgt gggtgtcttg gctcgtaagt atcatttgga
    16861 tgtttttgcc aatcagcaga cttgggaagc gatggaacca ctgattggaa atgtaccagt
    16921 ggaacaaaaa catctatttg agatggggaa agtcagaaca ttcggcgata tcgatattga
    16981 gagtttcggc gtctcccatg acgcagcggc tccgcaattt tatcgtttcc ataaagaaaa
    17041 tcggtcattc gtcatgttga cggacaccgg atattgcagt gattatatgc gaggtgtcat
    17101 tgaaaatgcc gatggttatt tgatggaaag taatcatgat cttgagatgt tacgcatggg
    17161 accttatccg tggaatttaa aacagcgtat tttaggagat agaggacatc tttccaacga
    17221 agacggtgca cttgtcatga ctgatgtgat cggtaatcgg accaaacgaa tctatcttgg
    17281 ccatcttagc aaggaaaaca atatgaaaga acttgctcat ttaacaatgg aaaatgtttt
    17341 gaaagaaaaa gattttggcg ttggacatga ttttgaaatc tatgatacag atccagatga
    17401 agcaactgag ctattttcga tttaaacagt tcaatcaata aaaatataaa aagcagaaga
    17461 taaaatcaat cagggttggt tttatcttct atttttattt ttttagcata ttaaaaaaat
    17521 aaccgtatta tcgactgact acactgatgt gctgcttttc atgtttctca tccagtatct
    17581 catctactaa tgcaatcgcg taatctgcca tgctgatcgt actgtcgcta gcagcgtttc
    17641 tttgtaaata atcatcactt aacaaataag tacctgtacg tttgccatgt ggatcaaata
    17701 aagaagctgg actgatataa gtccaatgta gatttttaga ttgtttcaat tttaacaatc
    17761 cttcgtacat attgaaagct gttggataga agggtgcatt tttaggtact tggtcaatca
    17821 tacgagtcgt cttttctgta tctaagaata acgaagaggc acccccaacg acaattaagc
    17881 gtgtatcggt tccttcaagc atctcaatca aatgggacaa agaagtttga tgcatttctt
    17941 cttttccttt gggagcgttg aaagcatcaa cgacaacatc aaaagatgca agatcagtag
    18001 ttgtcaactc aaataaatcc ttttgtataa acggaacatc tgatggcaat ttttctggat
    18061 gacgcacgat tgccgtcacg tctaaatctc tggacaatgc ttcgtctaaa ataaatgaac
    18121 ctgcatggcc agtagctcca atgagtgcaa ttttcataaa aaacctcctt ttttattcct
    18181 gtccattgta agcagttgct gaatattcgt caaagaatcc gatttttgaa aagctcagtg
    18241 cgcattcctt tcatggttcg tgatcattgg gtatagttaa attaagaaaa ctgattgata
    18301 aaaatagaag ttgaaggaga taagaaatga aagagtggtt agatcgtgcc tttttaccaa
    18361 tgataacatc tgttgtgaat cttagaccct taactgcttt gcgtaatgga atgacttaca
    18421 cgatcccaat aattattgtt ggctcgctgt ttttcttatt tgctcatttt cctgtggatt
    18481 acatcgctca gtggatagac aaaacaggat tttctatgta ttttacacaa gtataccagg
    18541 ctacatttga tgtgatagcc ttttggatcg tttttgccat tgcatacgct tatgcaaaag
    18601 aagaagacat cgatcgctta ccagtcggca taacagcctt gtctgcttat ctactgctta
    18661 ttcagccact aaatactcag atagatactg tctggacagg tgctgaaggg atactaggag
    18721 cagttacaac tgggctcatc atcggatggg gctatgcacg tttagtaaga cttgcgaaag
    18781 ggtggaagca aagagagaag atacctgaaa atgtttggca gtcttttact tcattgattc
    18841 caatcattgt tatagtcact tttacgctta tcggttcagc ggtttttcaa catttgttca
    18901 gaatgactgt tgttcaagga gtatggacat atgtccagct tcccattcaa tcgttcatag
    18961 acacgttgtt tggtgtgctt ttattaggtt tttttgttcc gtttttatgg ttatttggtc
    19021 ttcatggatc tgcgatggtc aatgggttgg ttagtcccgt cttgcaagcg aattcattag
    19081 caaatgcaga aatcttagcc tcaggacaag agttgacggt ggcaaatggc ggacatatcg
    19141 ttactcagca atttctcgat cagttcatga cggttacggg tgctggattg acgttagggg
    19201 ctgtattttt tatgacggtt tttgcgaaat cgaagaaata cagagaatta ggaaatcttg
    19261 ctcttttacc agcctttttc aacatcaatg aatcaattat tttttccaca ccgatcgtga
    19321 tgaacccgat gatggctgtt ccttttattt tcgcaccggt cgtttcagga ttgattacct
    19381 attccgcttt atattttgga ttcgttcctt tatttacagc agtccaagtc ccttggacaa
    19441 caccgccgat cctctcgggt ttcttagtag gcggagtgcc tgcagcgatt ttacaaggta
    19501 tcgtgttgtc aattagtttt ttcatttatt tcccattttt gaaaaaaata gattctgcaa
    19561 actgcaaaaa ggaaagacag acaaaaaatg atggagcagc agaaaagatg aacgactaaa
    19621 aaacaaaaat actcctgtcg ctttagattg ggaaaatcac caatctagga tgacaggagt
    19681 atttatagtc atgtttatac tagccctttt tggacagcta attgaacaag aacaactaat
    19741 gtgttcattc ctacgtgtgt gatcatcgat gtccagatcc gaccagttgc tttgtactct
    19801 aggctgaaga agaaacctag gaaaaagtag attagcagat gaccatcatt atgagcaaac
    19861 gcaaacagca aagagctgat cactgctccg acccaaaaat ttgaatatcg actgatgatc
    19921 cccagtatac tgcggcggaa tacgaactct tccatgatgg gtccagcaac agttgtcgcc
    19981 aaaataaaag cgggtgcttc catgatcatc tggatgatat tttgtgtatt ctcggaaggg
    20041 gttgctttgc caaacaatac agattctaac atgacagcga tgctttgcag aatcaatgcg
    20101 acaaagattc caaccaatcc ataaataaca atctttggag cagaagcttt ttttaccgct
    20161 gattcgatcg ctaagggttc acgttgctta aaataaaaaa tagctaggac aacggcgcct
    20221 agtatataca tgactgcagt tgctgtgatc aattcagacg tagattttac aagtcctatc
    20281 gaagctagga tcaagggtga gataaaaatt agaccatagc aaaaaatgct gaggaagcta
    20341 tattttttta aagacatgta attcctcctt ttaagggata acaagcatgc aaggcaagct
    20401 tatatcgatt gatttcaatt atatcataat ttcaataagc agctgtcaga ataaaatttc
    20461 ttttctttga tttaaaagct tgcaaaaaag agggagaatg agtattatta aaaatgtagg
    20521 ttagcactcg aaggttaaga gtgctaacag attaaaaaaa gagttatcgg agggatttat
    20581 cgtgttaaaa ccattaggtg atcgcgtcat tatcgaagtc gcaaaagaag aagaaaaaac
    20641 tgtcggagga atcgttttag cttctgctgc taaagagaaa ccccaaactg ggacagttgt
    20701 tgcagtcggt gaaggccgtt tgttagaaaa cggtgaaaaa gttcctgccg ctgtaaaagc
    20761 aggcgaccaa gttatgttcg aaaaatacgc cggtacagaa gtaaaatacg aaggaaaaga
    20821 atatctgatc gtcgcaggaa aagatatcat ggcgattgtt gagtaaattt ggaagataaa
    20881 aaaataatca aaaataatca atgaggtgaa tcaatcatgg caaaagaatt gaaatttgca
    20941 gaagacgcac gcgcagctat gttacgcggg gtagataaat tagcagatac agtgaaagta
    21001 acattaggac ctaaaggccg aaatgtcgta ctagaaaaat cttacggctc tccattgatc
    21061 acaaatgatg gtgtgacgat cgcaaaagaa atcgaattag aagatcattt tgaaaacatg
    21121 ggtgcaaaac ttgtatcaga agtagcatca aaaacaaatg atattgcagg agacggtaca
    21181 acgacagcaa ctgttctgac acaagcaatc gttcgcgaag gtttaaaaaa cgtaactgca
    21241 ggtgctaatc ctctaggcat tcgtcgcggg atcgaattgg caacaaaagc agcagtcgaa
    21301 gaattgcata atatctcaac tgttgttgat tcaaaagaag ccattgcaca agtagcagct
    21361 gtttcttcgg gttctgataa agttggacat ttgattgctg atgcaatgga aaaagttgga
    21421 aacgacggtg tcatcacaat cgaagaatca aaagggatcg aaacagaact agatgttgta
    21481 gaaggtatgc agtttgatcg tggttactta tcacaatata tggtaacaga taacgacaaa
    21541 atggaagctg ttctagaaaa cccatatatc ttgatcacag acaaaaaaat ctctaacatc
    21601 caagatattc tgccattgtt agaacaaatc ttgcaacaat cacgtccatt gttgattatt
    21661 gctgacgatg tagatggtga agcattgcca actcttgtat tgaacaaaat ccgtggaaca
    21721 ttcaacgtag tagctgtaaa agctcctggt ttcggcgatc gtcgtaaagc aatgcttgaa
    21781 gatatcgcta ttttgacagg tggtacagta attactgacg atctaggttt agaattgaaa
    21841 gatgcaacaa tcgaaaactt aggaaatgct tctaaagttg ttgtagataa agacaataca
    21901 acgatcgtag aaggttctgg agaaaaagaa gcaatcgaag ctcgtgtgca attgatcaaa
    21961 aaccaaattg ctgaaacaac ttctgatttt gaccgcgaaa aattacaaga acgcttagct
    22021 aaattagcag gtggtgtagc tgttgttaaa gttggtgcag ctactgaaac agaattgaaa
    22081 gaattaaaat tacgcattga agatgcattg aatgctacac gtgccgcagt agaagaagga
    22141 atggtctcag gtggtggtac agctctagtg aacgttatca gcaaagtttc tgctgtagaa
    22201 gcagaaggag acgtggcaac aggtatcaag atcgttgttc gtgctttaga agaaccaatt
    22261 cgtcaaatcg ctgaaaacgc tggttatgaa ggatcagtga ttgttgataa attgaagaac
    22321 gtggaactag gcactggttt caatgctgct actggagaat gggtaaacat ggttgaagca
    22381 ggtatcgtag acccaactaa agtaacacgt tctgccttgc aaaatgccgc ttccgtttct
    22441 gccttgttat taacgacaga agcagtcgtt gcagataaac cagaaccagc agcgccagca
    22501 gctcctgcaa tggatccatc aatgatgggc ggtatgatgt aggaattaaa agagaaccct
    22561 tatattacag ggttctcttt tctttttttg ttttatttta agtaaaaggg gcaaaaaagg
    22621 ggcagaaata tcaaagccta tttagtttat caattacttg ttgttttgct tttttggtta
    22681 cgtgattgta aatagacaga gttgtatttg catcagaatg tcctacacgc tccataatgg
    22741 ctttaagagg tacgcctaat tcagataata gcgaaacatg gctatgtcta aatatatgcg
    22801 atgatagatt tttttctaat tccaactctt cttctacttt atggagtaca gcgttaaatg
    22861 agtgcagcgt aagcggagtg ccatttgtag atataaatat atattgatcg ggattggtgg
    22921 gtctacctgc aagaatattg tcagctatca cactttcaat caattctttt gcacgattgg
    22981 ataattgtac ttcacgttgt gagtaagtat tttttggagt tgtttttatg gcgttatcca
    23041 tttttacaga tgtgtaatct aaggtcccat taatggaaat tttaccctct ccatagtcct
    23101 tcatttgcaa agccagcaat tccccatatc tcaaaccagt caaatataga aattcagcta
    23161 ttatgccgtg tagttttctg cgaggattgg aatataactg tttcagtatt tgatcaattt
    23221 cttctttctc cagatatttt ttatccatag aaagctttct tttttcttct tccacttttt
    23281 taggatggat tttaactgct agagcagggt ttctttgaat gtattttcta tcaattgcat
    23341 agtttagcat aacggataga gttgtttttg tttgctttgt gtagttcagt gagagatcac
    23401 caaaagtgta catatcttca atcatcttat taatgagtgt ctcatcaatg tttctaacga
    23461 ttgtatcatc gcttatgtgc ttagaaacat gtttcatcat cattggaacc ttcaaatagc
    23521 tagtacgttt aacatgctgc ttataatatt cataccattc tttatacagc tcaccaaaag
    23581 tgatatctga tttattgtaa ttttcgagtg cttctttaat ttttttatct agaattttct
    23641 gagctttttt ccacgcttgt ggtgaattac ttgtaagtgt tgtagatttt tttcgtgttt
    23701 tttctgtata aggatctaca tatctttcaa taaacttgaa ttggccatct ttggtttgtt
    23761 caacccacac ttttaacatc tcctatcatt tgctataata ggcataacaa atagacctat
    23821 ataggtttgt tttctaaaag cacgctctta ctctggacgg tggggcgtgt tttttattta
    23881 ttatacagtt gctttaaaaa tagctgcttt ttcaaatgat actaaaggag agaagtggat
    23941 ttctatttct cccttttggt ttaacgtgaa atgagcagtt acatccattt gttttcctgg
    24001 agcaacagat cccattgtat tttcgttggc atatgtctct gattttttgt catctggtcc
    24061 atatacttcc acatctgtac ctacagggat atctgaatcg ccatcatttt ttacggtata
    24121 agtaattttt actacttgtg caggttggtt ttcttcaaat tgatttcttt catcagttag
    24181 ttctacacta tttagtgtat attcagcatc accaactttt acagtgtcac caatcttata
    24241 gaaggtatcg cttttttctt ctttagaaga tgaagcagag gttgaagatt tagttacttt
    24301 ttcgccacca ttatcacttg ctttattact atctgatcca ccatttaaag cagaaccaat
    24361 aattataatt aaaattactg ctaataccca aaaccatact tttttgtaaa aaggtttact
    24421 aactttatac atttttccgt cttgacccat aacttttttt gccatttaaa tattcctcac
    24481 ttcttgttat aatatatttg cgatctcaga aatgaggtat gagtccgtgt g
//
LOCUS       DAZUMT010000033        24257 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000033 DAZUMT010000000
VERSION     DAZUMT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.033"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..995
                     /locus_tag="ACG8FM_001216"
     CDS             138..995
                     /locus_tag="ACG8FM_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS5093889.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1110..2030
                     /gene="pstC"
                     /locus_tag="ACG8FM_001217"
     CDS             1110..2030
                     /gene="pstC"
                     /locus_tag="ACG8FM_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS5093890.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2030..2914
                     /gene="pstA"
                     /locus_tag="ACG8FM_001218"
     CDS             2030..2914
                     /gene="pstA"
                     /locus_tag="ACG8FM_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS5093891.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2929..3735
                     /gene="pstB"
                     /locus_tag="ACG8FM_001219"
     CDS             2929..3735
                     /gene="pstB"
                     /locus_tag="ACG8FM_001219"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5093892.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3751..4509
                     /gene="pstB"
                     /locus_tag="ACG8FM_001220"
     CDS             3751..4509
                     /gene="pstB"
                     /locus_tag="ACG8FM_001220"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5093893.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4522..5199
                     /gene="phoU"
                     /locus_tag="ACG8FM_001221"
     CDS             4522..5199
                     /gene="phoU"
                     /locus_tag="ACG8FM_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS5093894.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5437..5736
                     /locus_tag="ACG8FM_001222"
     CDS             5437..5736
                     /locus_tag="ACG8FM_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093895.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5777..6469
                     /locus_tag="ACG8FM_001223"
     CDS             5777..6469
                     /locus_tag="ACG8FM_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5093896.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6466..7233
                     /locus_tag="ACG8FM_001224"
     CDS             6466..7233
                     /locus_tag="ACG8FM_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5093897.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7333..8901
                     /gene="liaX"
                     /locus_tag="ACG8FM_001225"
     CDS             7333..8901
                     /gene="liaX"
                     /locus_tag="ACG8FM_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS5093898.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9198..9518
                     /locus_tag="ACG8FM_001226"
     CDS             9198..9518
                     /locus_tag="ACG8FM_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS5093899.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9518..9868
                     /locus_tag="ACG8FM_001227"
     CDS             9518..9868
                     /locus_tag="ACG8FM_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS5093900.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10035..10973
                     /gene="hprK"
                     /locus_tag="ACG8FM_001228"
     CDS             10035..10973
                     /gene="hprK"
                     /locus_tag="ACG8FM_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS5093901.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10988..11818
                     /gene="lgt"
                     /locus_tag="ACG8FM_001229"
     CDS             10988..11818
                     /gene="lgt"
                     /locus_tag="ACG8FM_001229"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5093902.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11862..12887
                     /locus_tag="ACG8FM_001230"
     CDS             11862..12887
                     /locus_tag="ACG8FM_001230"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5093903.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12902..13840
                     /gene="galU"
                     /locus_tag="ACG8FM_001231"
     CDS             12902..13840
                     /gene="galU"
                     /locus_tag="ACG8FM_001231"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5093904.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13965..14405
                     /locus_tag="ACG8FM_001232"
     CDS             13965..14405
                     /locus_tag="ACG8FM_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS5093905.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14408..15010
                     /locus_tag="ACG8FM_001233"
     CDS             14408..15010
                     /locus_tag="ACG8FM_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5093906.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15078..16184)
                     /locus_tag="ACG8FM_001234"
     CDS             complement(15078..16184)
                     /locus_tag="ACG8FM_001234"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS5093907.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG8FM_001235"
     CDS             16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG8FM_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS5093908.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17731..20076)
                     /locus_tag="ACG8FM_001236"
     CDS             complement(17731..20076)
                     /locus_tag="ACG8FM_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS5093909.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20355..21257
                     /locus_tag="ACG8FM_001237"
     CDS             20355..21257
                     /locus_tag="ACG8FM_001237"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS5093910.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21470..23245
                     /locus_tag="ACG8FM_001238"
     CDS             21470..23245
                     /locus_tag="ACG8FM_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS5093911.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23502..>24257
                     /locus_tag="ACG8FM_001239"
     CDS             23502..>24257
                     /locus_tag="ACG8FM_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5093912.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         7748 a         4334 c         5089 g         7086 t
ORIGIN      
        1 tagcatagaa acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat
       61 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
      121 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcca
      181 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
      241 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      301 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      361 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      421 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      481 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      541 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      601 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      661 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      721 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      781 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      841 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      901 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      961 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
     1021 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
     1081 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
     1141 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1201 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1261 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1321 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1381 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1441 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1501 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1561 gaagtatttt tggcgggaca gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1621 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
     1681 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1741 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1801 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggactta
     1861 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1921 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1981 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     2041 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     2101 tttagcagct ttactacttt atatcttagt tcggggaata ccgcatattt catggtcctt
     2161 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
     2221 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2281 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2341 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2401 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2461 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2521 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
     2581 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2641 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2701 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2761 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2821 cgcttctgcc gtgttgatca ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2881 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2941 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     3001 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     3061 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     3121 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3181 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3241 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3301 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3361 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3421 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
     3481 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3541 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3601 caagcagctc gtatcagcga ctatactgcc tttttctatc taggaaaagt aatggaatat
     3661 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3721 ggtcattttg gttaagacgg aggcctcaac atgacaaaag aaattatttc atctaaagat
     3781 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcaatctaag tttcaatcaa
     3841 ggggaaatca ctgcgatgat cggtccttct ggttgcggga aatccactta tctgcgttct
     3901 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3961 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     4021 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     4081 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     4141 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4201 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4261 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4321 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4381 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaacgat
     4441 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4501 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4561 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4621 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4681 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4741 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4801 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4861 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4921 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4981 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     5041 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     5101 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     5161 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5221 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
     5281 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
     5341 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
     5401 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
     5461 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat
     5521 gaagtacctg tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca
     5581 ggaagactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
     5641 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
     5701 ggagatgaat ggcagctagt agcaaaaaaa aaatgatggc tggcttttct tcttcacaag
     5761 tatctccatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg ctttaggaag
     5821 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5881 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5941 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     6001 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     6061 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6121 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6181 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6241 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6301 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6361 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6421 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6481 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6541 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6601 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6661 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6721 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6781 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6841 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6901 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6961 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     7021 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     7081 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7141 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7201 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7261 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7321 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7381 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7441 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7501 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7561 agagaaaggc aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7621 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7681 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7741 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7801 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7861 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7921 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7981 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     8041 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     8101 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8161 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8221 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8281 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8341 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8401 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8461 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8521 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8581 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8641 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8701 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8761 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8821 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8881 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8941 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     9001 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     9061 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9121 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9181 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9241 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9301 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9361 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9421 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9481 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9541 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9601 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9661 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9721 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9781 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9841 tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9901 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9961 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    10021 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    10081 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10141 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10201 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10261 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10321 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10381 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10441 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10501 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10561 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10621 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10681 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10741 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10801 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10861 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10921 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10981 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    11041 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    11101 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11161 tatctccatt attggtgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11221 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11281 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11341 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11401 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11461 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11521 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11581 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11641 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11701 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11761 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11821 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11881 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11941 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    12001 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    12061 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12121 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12181 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12241 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12301 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12361 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12421 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12481 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12541 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12601 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12661 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12721 ctactcaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12781 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12841 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12901 tatgaaagtc aaaaaagcag ttattccagc ggcaggatta ggaacaagat ttttaccagc
    12961 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    13021 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    13081 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13141 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13201 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13261 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13321 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13381 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13441 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13501 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13561 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13621 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13681 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13741 gtatatcctg tctttaggta aaaaattaga agcagaagga agcaagatcg atcataatcc
    13801 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13861 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13921 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13981 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    14041 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    14101 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14161 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14221 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14281 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14341 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14401 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14461 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14521 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14581 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14641 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14701 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14761 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14821 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14881 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14941 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    15001 aaatcactaa aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc
    15061 tctcaccttt tttactctta tttttctaaa actagtaatt cttttggtgt agtcgtgaat
    15121 ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca
    15181 ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt
    15241 ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga
    15301 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    15361 cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg
    15421 ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca
    15481 ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc
    15541 ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    15601 tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc tgcaatgatc
    15661 gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc
    15721 catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt
    15781 tggatgatgg aagctagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca
    15841 tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag
    15901 cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg
    15961 acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg
    16021 aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg
    16081 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt
    16141 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    16201 taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa
    16261 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat
    16321 tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    16381 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    16441 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    16501 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg
    16561 tggcttggca agtaagaaaa caacaacagt tggggtaatc atccctgatg tcagcaatat
    16621 gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat
    16681 tatcttagca aattcagatg gtaacgacca aaaagaagtc aatgtattga ataacttact
    16741 ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg
    16801 agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca
    16861 agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa acaaattggc
    16921 aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ctatcaatgg
    16981 acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga
    17041 aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat
    17101 ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt
    17161 ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa
    17221 caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata
    17281 cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    17341 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa
    17401 ggacaaccat tcagaggttg tgagcctgcc gtttaactct gcgttacaag gagcaatttc
    17461 aaaaaacagc atttcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga
    17521 acaccgttta ttcgggaaaa ggggttatga caaaattttg tttaatcctt tttttttgtc
    17581 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    17641 gatgctgcat tatgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    17701 cagtccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttggt
    17761 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    17821 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
    17881 ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
    17941 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
    18001 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
    18061 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
    18121 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
    18181 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
    18241 atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt cattcatgat
    18301 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
    18361 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
    18421 taaagcctga aatccattgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
    18481 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
    18541 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
    18601 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
    18661 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
    18721 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
    18781 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
    18841 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
    18901 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttctacgcc
    18961 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
    19021 ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
    19081 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
    19141 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
    19201 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
    19261 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcacc
    19321 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
    19381 ttgcggcagc gtcacatctt ttgcatctac accaaagatt cctcttgcag cttcctggac
    19441 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
    19501 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
    19561 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
    19621 agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc
    19681 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
    19741 tttcaataaa ggtgaaattt tatcagatga aatagtcgtg cgcatcagat ctgtctggat
    19801 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
    19861 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
    19921 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
    19981 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
    20041 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    20101 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
    20161 aatttctttt ttaaaagagt taaaacgtga gaaaatgatc ttttttataa aagagttctt
    20221 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
    20281 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
    20341 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    20401 taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
    20461 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
    20521 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
    20581 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
    20641 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
    20701 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
    20761 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
    20821 catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
    20881 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
    20941 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
    21001 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
    21061 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
    21121 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
    21181 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
    21241 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
    21301 cttgtccttt tttgtttaag aaacaaagga gtgctttttg cagtcttttt catcatgcat
    21361 cttctccccc ctctttttaa atttagacaa acagggtata ctggtagaaa agtaaaaaaa
    21421 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
    21481 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
    21541 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
    21601 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
    21661 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
    21721 agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt
    21781 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
    21841 aagtttctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
    21901 aacgtatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
    21961 tatttattac cgtaaatata gttccagcaa aaaaatgctg ccgtggtttt tagggctagc
    22021 tgtttctttt cctttctttt ggttcattcg cgaagattcc atctggcttc ttccttttac
    22081 tgtagtcgcg ttaggcatca gtttcgtcca gatcttgcta ggacaagact tttctttcaa
    22141 agaagcaaag aagttaaaag aacgaccatt tctcctttcg aaacaagaat ggctgaaact
    22201 ttcattgttt ttactccctc tagtcggttt ctccatcgtc tatcagtgga tcgaatataa
    22261 aaacgagaca acatatagta ttgccgccat caatgaccgt tcgagtggcg aatttggacg
    22321 tttgatgaaa cagctgatcc gaatagatga cgaaacgaac ttaaataaag aaaacagtga
    22381 gatatgggtc tcgcatgctg cattaaataa agcattagac gcatctccta catttcatgc
    22441 tgtttctact cgtattgacg aattgtatac gactcatgcg tggacccaag gtggaaaagt
    22501 aaaagaatta ccaggagata tcattttttg ggcattgcga gatgtggtaa gcgaatctgg
    22561 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca atcaagaatt
    22621 agaagaagcc tatcaaaagg gaactatcaa aaagaaaaaa gaattctatc tgatgggatc
    22681 aggggatgga aagacgatga atgatctgcc aatcgtattt gcttttctga aatcaggata
    22741 tgcctatgca tggcgataca aagatttcca tcaagggggg aattatagtt tcggtacgca
    22801 agaacaagtc gaacaggccc aagaattatt gaatcttcca ctaatggata attggatcga
    22861 tagcagtgat ccgagaacac ctgatttcca gttgacaaaa acagcaaaag tcgcgaatgt
    22921 catgataaaa atttaccaag ttctggccat tcccgtgctg attgcttcaa tagcagggat
    22981 attacttatg atactaggaa ccgcatttgc aaaagctgat cgtgcctatt atagttcctc
    23041 tttgattgta gtcaccggat taatactgac acaattggtt tttcttttcg gcgtctcgtg
    23101 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
    23161 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattgaatct
    23221 gaaaaaagct ttatcttcca aataaaaaca tactaagatt agaggagaac atcattgacg
    23281 aatgatgttc tcctctattt tctattttta gattatagtg aagagtgaaa gaaagttcga
    23341 acaaagcctt gaggactatg agtccctatc aaaaaccgaa cttctttact gtcattttag
    23401 aatcaggttt acgtatgttg gggccatgag cccgtgtata ggaaattgga atcgaaatga
    23461 agcagtaaag ttctttcaaa acttaaaaag gaggaaaaca aatgccttac attcttgcat
    23521 tagatgtttc aatgggacac agctacagtg tcctatacaa agacgacacc tgcttatttg
    23581 aagacgagat cgaacacact caaagaggat ttcatgcatt actcgaagag atccagcact
    23641 tgcctgaacg tccattgatt gtttttgaag caacggggat ttattccaga ccagtcgaaa
    23701 agttctttca agatcatcac ttttcttact gtttgttaaa cccgttagaa gcgaaaaaac
    23761 agatggaaga aaacacctta tgcagttgga aaacagataa atcggatgcc catcggctcg
    23821 cacagactca tcttaaaaat caacgtcagc caaaagaagt acaaagcaat ttttacttag
    23881 agatgcgcga tttagcccgt ttttatcaag aaattgagaa gaaaatcact cgtctgcgca
    23941 tggatttaca caactgcctt cagttgactt ttccagagct tgaacagttc tttttaaaca
    24001 ggctgacccc ttacgcattg atattgatcc gtctttttcc ccatccagat tttgtattgg
    24061 catctactcg gacaaaaata aaaaataaac tgatcaacga aacacggaaa aaaatctccg
    24121 cgaatcgtgc cgagcaaaag gcagatcaaa tcatccacta tgcacagtgt gcctatccag
    24181 ctgttgaaaa agacagtatc cactgtcaaa aaacgatcta ttacgctgaa cttctccaag
    24241 atttactcga acaaaaa
//
LOCUS       DAZUMT010000034        24255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000034 DAZUMT010000000
VERSION     DAZUMT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.034"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACG8FM_001240"
     CDS             complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACG8FM_001240"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS5093913.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACG8FM_001241"
     CDS             complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACG8FM_001241"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS5093914.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACG8FM_001242"
     CDS             complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACG8FM_001242"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS5093915.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3371..4678)
                     /locus_tag="ACG8FM_001243"
     CDS             complement(3371..4678)
                     /locus_tag="ACG8FM_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS5093916.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4681..5262)
                     /locus_tag="ACG8FM_001244"
     CDS             complement(4681..5262)
                     /locus_tag="ACG8FM_001244"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS5093917.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5378..5475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5834..7126)
                     /locus_tag="ACG8FM_001245"
     CDS             complement(5834..7126)
                     /locus_tag="ACG8FM_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5093918.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7123..7746)
                     /locus_tag="ACG8FM_001246"
     CDS             complement(7123..7746)
                     /locus_tag="ACG8FM_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093919.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7749..8585)
                     /locus_tag="ACG8FM_001247"
     CDS             complement(7749..8585)
                     /locus_tag="ACG8FM_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5093920.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8602..9489)
                     /locus_tag="ACG8FM_001248"
     CDS             complement(8602..9489)
                     /locus_tag="ACG8FM_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5093921.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9505..10788)
                     /locus_tag="ACG8FM_001249"
     CDS             complement(9505..10788)
                     /locus_tag="ACG8FM_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5093922.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11471..12154)
                     /locus_tag="ACG8FM_001250"
     CDS             complement(11471..12154)
                     /locus_tag="ACG8FM_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS5093923.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12172..12789)
                     /locus_tag="ACG8FM_001251"
     CDS             complement(12172..12789)
                     /locus_tag="ACG8FM_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5093924.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12799..13623)
                     /locus_tag="ACG8FM_001252"
     CDS             complement(12799..13623)
                     /locus_tag="ACG8FM_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS5093925.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13616..14176)
                     /locus_tag="ACG8FM_001253"
     CDS             complement(13616..14176)
                     /locus_tag="ACG8FM_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093926.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14350..15306
                     /locus_tag="ACG8FM_001254"
     CDS             14350..15306
                     /locus_tag="ACG8FM_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5093927.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15611..16567)
                     /locus_tag="ACG8FM_001255"
     CDS             complement(15611..16567)
                     /locus_tag="ACG8FM_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS5093928.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16671..17273
                     /locus_tag="ACG8FM_001256"
     CDS             16671..17273
                     /locus_tag="ACG8FM_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5093929.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17361..18860)
                     /locus_tag="ACG8FM_001257"
     CDS             complement(17361..18860)
                     /locus_tag="ACG8FM_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS5093930.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18876..19400)
                     /locus_tag="ACG8FM_001258"
     CDS             complement(18876..19400)
                     /locus_tag="ACG8FM_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS5093931.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19698..20447
                     /locus_tag="ACG8FM_001259"
     CDS             19698..20447
                     /locus_tag="ACG8FM_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5093932.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20438..21223
                     /locus_tag="ACG8FM_001260"
     CDS             20438..21223
                     /locus_tag="ACG8FM_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5093933.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21192..21908
                     /locus_tag="ACG8FM_001261"
     CDS             21192..21908
                     /locus_tag="ACG8FM_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5093934.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22010..23029)
                     /locus_tag="ACG8FM_001262"
     CDS             complement(22010..23029)
                     /locus_tag="ACG8FM_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS5093935.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23048..24049)
                     /locus_tag="ACG8FM_001263"
     CDS             complement(23048..24049)
                     /locus_tag="ACG8FM_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS5093936.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7620 t
ORIGIN      
        1 ttgctattct attttaattg aattttgcaa ctcactcttc tttattccaa acctactcgt
       61 ttgaatatca cgtcaacgtt tttcaaatga tgatgataat caaaggcatc gtctatttct
      121 tctttagata ataccgatgt gatggttgga tcttcttcta acagcgggcg gaactgcact
      181 tggttgtccc aagcgtacgc agtttttggc tgaacaagat cataagcttc ttctcttgtc
      241 attcccttat cgatcaattt cagcataacg cgctggctat aaatcaaacc aaatgttgca
      301 gacatattcc gcttcatgtt ttcaggaaag accgttagat ttttaacgat attgccaaaa
      361 cgatttaaca tgtaattcaa taaaatcgtg gtatcaggca agatgattcg ttctgctgaa
      421 gaatgagaaa tatctctttc atgccataat gtgacatttt cataggcagt gaccatgtgt
      481 ccgcgcacca ctctggctag accagccata ttttctgaac cgatcggatt ccttttgtgc
      541 ggcatagcag aagaaccttt ttgacctttc gcaaaaaatt cttctacttc acgtgtttcg
      601 gatttttgca atccgcgtat ctctgttgca aaacgttcaa tacttgtagc aataagcgcc
      661 atcgctgaga aatattcagc atgtagatcc cgagggagaa cttgagtaga gatttcttgt
      721 ggacggatcc ccaattgttt acatacatac tcttcaataa atggcgggat atttgcgaac
      781 gttcccactg ctcctgagat ttttcctgct tctactcctt ttgccgcatg ttcaaatcgt
      841 tcgatgttcc gtttcatttc tgaataccat gtggcaagtt tcaatccaaa agtagttggt
      901 tcagcatgga caccgtgtgt ccgacccatc atcactgtat atttgtgttc ttttgctttt
      961 tcgccaatga tttccgtaaa acgtttcaag tcttcccgta aaatatcatt tgcttgcttg
     1021 atgagatagc cataagcagt atctacgaca tccgtacttg ttagaccata atggacccat
     1081 tttctttctt ctcccaatgt ttcagaaacc gctcgtgtaa acgcaacgac atcatggcgt
     1141 gttaattttt cgatttccaa gatccgatcg atatcaaatg atgcatgctc acgaattttt
     1201 ttcacatctt cttgcgggat ttcaccaagt tctgcccaag cttcatctgc taaaatctcg
     1261 acttcgagcc acgcacggta tttgttctct tccgtccaga tattccccat ttcagggcgt
     1321 gtataacgtt ctaacatgtt ttttctcctc taatcccaaa tattcgtttg tttgatttct
     1381 tccagcgttt gctggatgtc ttctgtcaag atagtgatat gtcccatctt gcgacctttt
     1441 tttgcttgtt tttttccata gaaatggaat tgccagtctg gcttattctc aatcaagcga
     1501 tagctttcca gcagttgttc tcccaaaaca ttgaccatta ctgcattgct tatcaaacgt
     1561 actttttctc ctaatggcca accacaaata cctcgaatat gggcatcgaa ctgactcata
     1621 gaacaagctt caatagaata atgtccagaa ttgtgaggac gaggagctaa ttcattcaca
     1681 tatagcccgc ctgtttttgt taaaaacatt tctaccccta aaacacctga aaggctcact
     1741 gcttctgcaa tcacacgggc aatacgctca gcctcctcga tcacatcctg atcaatcgcg
     1801 gctggagcaa tcgtttcatg aagaatattg ttgtggtgga tgttttccac gaccggaaaa
     1861 gtcgtgaaat ctccttgacc atttcctgcc accatgatcg aaatctcttt ttcaaacggg
     1921 atccatgctt ctaaaacaca ggttccttct cttaaaaggt ccatggcagg agctagatcc
     1981 gcccgacttt tcaaaacata ctgccccttg ccatcatagc cgcctcgagt ggttttcaaa
     2041 acacaaggat agccgatccc gtcaatcgca tcttggatat ccgtcggact gacgatcgtt
     2101 gcataaggag caatcacgat gttattcgtt tccaaaaaag atttttctaa caggcggtct
     2161 tgcgtaatcg caagcaagtc tgtcccttga ggaataaatg acatgggtaa aatcgcattt
     2221 aacgcctcta cactgacgtt ttcgaattca taggtaatga catctgttct tctggcaagc
     2281 tctttcagcg caaaggtgtc atcatagaca ctttcaatgt gccaatctgc aacttgtgcg
     2341 gctggacaat cgctcacagg atccagcaca cctactttaa agcccatttc ttttgcacta
     2401 atcgccatca tacgacctaa ttgaccgcca ccgacgatcc cgatcgttga accaggcaac
     2461 aaaggtttaa ccaagttgat cactactttc tataacagtt ttagccaaca ttgaacgtct
     2521 ttcagccaat ttagcctcca attctaaatc atacatggaa agcatctgta tcgcaagtag
     2581 tccagcatta attgctcccg cttttccgat tgctgtagta gctacaggta ctcctccagg
     2641 catttggaca atcgaaagaa gcgaatccaa accatttaat gttcgtgatt ggaccggtac
     2701 accgataacc ggcaaagttg tttttgatgc gaccataccc ggcagatgcg cggctccccc
     2761 tgctcctgca ataatgattt tgattccttt ttttcgagcg ttttgtgcat attcaaacat
     2821 caaatctggg gttcgatgag cagaaacgac ctttttttca taagacacac cgaattcatc
     2881 caatatttga catgcttctt tcattgtttc ccaatctgaa gtactcccca taatcacaga
     2941 gatttttgct gtcattgttt ttcctcccag tttttctaca ttcatatttt aacaatcact
     3001 ttttttgatg tcaaagaaat ttcgaatgtt aacaaagaaa aacattttat tgttcgtgtt
     3061 tattaaagat aaaaaaaagg aatgtgacaa aagttaggtc acattccttt ttatggataa
     3121 acggtgttct cctgccaact cctcggtcac aaggaacaat attcaacaaa catgagaagc
     3181 ttttttttga aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttcct
     3241 tcaacggtgt tcaatcagct gaccctcggt attagctcaa acaaacggca aaatacgagg
     3301 aactattttt ccgtttgttt ctctaacaat caggtcaaaa cgctgatttc acaacctttc
     3361 ttttgctcta ttacttcttg aaggcttctt ccaatatttc ttcatctttt ttcaatccat
     3421 taaagatcaa attcaatatg atagccgtaa tgctactcat tacgatgcca tttccagtga
     3481 acatttgcaa tgtttgcggc atttgactga ataatgtcgg cgtaacattg aaacccagcc
     3541 caaatccaac agaaacagca acaatcaata gatttttatc attgctgaaa tcaacttcca
     3601 acagcatacg gattccttgg actgccacca tcccgaacat gactaacatt cctccaccaa
     3661 gtaccggctc agggatgatt tgtgctaacg caccgatttt agggaataat cctaaaacaa
     3721 tcaagaagaa tgcagagaaa tagatgggtt ttcttgtttt gattcccgaa agttgaacca
     3781 atccgacatt ttgagaaaag ccagtataag ggaatgtatt gaaaataccg cctaagataa
     3841 cggcaagtcc ttctgcacga tatccctttt tcaattcttc ttctccaaca tttttcccag
     3901 taatgtcacc aagtgcaaaa tatacacctg ttgattctac catgctgacc atggaaatga
     3961 tgatcattag taaaatcgac catacgtcga aagttggttt accaaaataa aagggttgtg
     4021 gaacatggaa caacggcgca tctccaatcg cgctcagatc aatcattccc atgaaagcag
     4081 ctaagatgct tcctccgact aaaccaatca ataccgcgat tgaacgaatg aacccttttg
     4141 ccagcatttg cacgcctaca attaggagaa tcgtcacaaa tgcaagtagc agatttgttt
     4201 tgtcaccaaa atcagctgct gttgcattac ctccacccat tttctcgatt gctacaggaa
     4261 tcaaagtcaa accaataacg gtgataaccg ttcctgttac tataggaggg aatagttttt
     4321 taattttaga aaagacacca gaaactaaca caacaaagat cccagaagcg ataatagatc
     4381 cgtagatagc tcctactcct tcattggttc cgatcaagat cagtggagct actgcttgga
     4441 tcgcacatcc taaaacgaca ggcaagccaa ttccgaaaaa tcggttaact gtcaattgca
     4501 ataatgtcgc taatccacac ataaagatat caattgaaat caaataagtc atctgttctt
     4561 gattaaagcc tagtcctgta ccgatcaaaa gaggcaccgc tactgctcct gcatacatcg
     4621 ctagcagatg ctgcagccct aaaaccgcag cttttccatt ttgtgtttct ttttccacga
     4681 ttatgcgtcc tcctcaagaa actcaacttg tccattcgtc aaggaagcaa tacgcgcaag
     4741 agaaacgact ctcaatccca tttcttcaag taactgacgt ccatcttgga aagacttttc
     4801 gatgacgatc ccgattcctt ctacttgcgc tccagcttgc tggcataatt cgatcaatcc
     4861 ttttgccgct tgtccgtttg ccaagaaatc gtcgataatc aatactttgt cttctgaaga
     4921 taaaaattta cgagaaatag aaatcgtact tgtcacttgt ttcgtaaaag aataaacaga
     4981 agctgtcaat aattcttcat ccatcgtcaa actttttgct tttctagcga agatcattgg
     5041 aacatctaat tctcttgcag caaataaagc tggtgcgatc ccagaagctt caatggtcac
     5101 aactttcgtg attccttgtt ctttaaatac ttctgcaaac cgttttccca tttgttccat
     5161 caatacaggg tctacttggt gagtgacgaa gctgtcgact tttaatacgc cctcgcctaa
     5221 tatacgtccg tctttttgaa tacgttcaac taattctttc actttatttg ctcctttatc
     5281 atttattaat accgaaataa ccgcttttgt gcaaaaacct actatgtttc tgttaaagaa
     5341 aaacaccctc cactttatca taagaaaaga agggtgaaat agccaatctt ttacttatag
     5401 tccagcattt acggtactgg ctagaaactg tcatccatat tatgacgata tataagtagg
     5461 tatgtaaaac taataatagt gtaaattcat tttcttgcaa ctgttttgca cgtgaaggca
     5521 gtttgccata tttgaataaa aaatgttgtt tcaaatattg aaaacagtct aacactactt
     5581 tctgaaactg acaacgtggt ttacttattt ttttgtaaaa tctaatgatt tttaatgcat
     5641 tattcctttt tgttcgggtt taaatagtta tttttatttt ctcctatcta aaaaagcagc
     5701 ccacataaaa agaaggcatg acaaaaagca tgtcatagcc cctttttccg aataaatagt
     5761 gttcaaaaga cagcctatcg gcaataaggc aagattttat aaactacggg aagtaatttc
     5821 cgtaaatcta ttcttattct tttaagctga caagctcgtc aatcactact gcttttcctg
     5881 aagcaataga atgatttgct gcgattcctg tcaaaatcga caaaatacca tctacgtgag
     5941 ttgctgcccg attccacgga tcttcttctt tttcttcacc aaatagatca tctaataaac
     6001 gcggatctcc tccgccatgt ccgcccgctt cttgttcgac gattacctca tatggctcac
     6061 cgaacatcgg atagacatgg atggactgaa gaacggctgc tccttcatcc tttttatctc
     6121 cacctgcatt gatgtaagac ttttcgatca ctttcatttc gatccgtcct ttgctgccat
     6181 tgaaagaaac attgaatcct tcccacggca tatacgcgtt tagcgaatac gtcaaaatcg
     6241 caccagtttc atatttgacc gttacgccta gtgtatcttc aatagagata tcatcggaaa
     6301 atacactgcg gtcacgaata tatccactgt ctttttctgc ttctaaatac atttctcgca
     6361 attcctcatt ggctgacagg tctatagcaa atggatcagc tttagctagt tcgcttccat
     6421 agcatcgact gtaaaatatt tctactccgc gtttctccgc attatgcatc ccataaaagt
     6481 ttaatccacc gaatgcaaat accttttggg gacggctgtt caaccaaaaa ttcactagat
     6541 caaaatgatg ggtagattta tgaacaagaa gcccgccgct attgtgctta tttcgatgcc
     6601 atcttctgaa ataatctgcc ccgtgttgcg tatttaacag ccattcgaag tggatagaaa
     6661 acacatcacc aatcacatca ctttctatca gttcctttat cttcgtgtga tacggtgcat
     6721 aacgataatt aaaagtgact cgcaggcttc ttcccgtctg tttttggacc gttattattt
     6781 cttgagcttt ttcctcatcg atagtcagag gtttttcgca aataacatca tatccttttt
     6841 tcattgcacg aatgatataa tcatgatgcg ttcgatctac tgacgtaata atgacttcag
     6901 ccggccgtac gtcatccagt agttgctcaa attgtgatgg catacatgtc tttacttttt
     6961 tctgtccata cttgttcaca agtatccgat ttgcataatc catccttgtt tgagatacat
     7021 cacaaaaacc gactagttca caagtttgat gatacgtaga ggcaattgcc tcataaaaaa
     7081 aacgcgctct cccaccaagt ccgacttgta catatttttt cttcatttaa agtcattctc
     7141 cttttctttt aagcaaaata ctgtgacaaa tggcagccct ggatacagta atgcagtgag
     7201 taaaaaatca tgctgtatac accagaacag tccatacaaa aggcataaat accacagaaa
     7261 ccccttgacg ttttttgaca acagccagat attccttttg atcaaataaa caagactatc
     7321 tgctttatct tctttgcaga tatcaattag cgtactaaaa aaactatggg atacaaaaag
     7381 caacagacac aatagaaaag gaatcagata ccaagctcca atctttccca ctaataaata
     7441 ccaatcaact gcaaaaataa tgacaaacag ctgaaacaac gtaatgatga aacacatccg
     7501 tttgattttt cctttcctcc aacatacctt tttttctgcc aagtaatgga cagatagata
     7561 ataagaagga tacatcatca aaaaataaat cagatagaca acacgagcag acagcactaa
     7621 tcctaaagtg aacaaacatc ctagaactaa agcgaaaaga gcattactga taagactggt
     7681 taaaaaaatc cgacagacat agtagttgag cttgtaaaga aacatctctt ctattggctt
     7741 tttcatattc agcccttcag accgcttgta ctgatcccct ggacaagata tttattgaag
     7801 aataagaaaa tcagaaaaac cggtagcagc gacaacgtag acatcgcaaa catactcgcg
     7861 taagtcgtac tagatccagc gtctgcaaat aatttgatgg caatcgatac cgtgtaagat
     7921 ctcggcttgt ttaaataaat caatggtccc atgaagtcgt cccatttcca ataaaactgg
     7981 atgatgatcg tcgtgatgat cgaaggtttc agcaaaggaa aaataatttt ggtaaagaca
     8041 ctatacttgc tgcatccgtc aataatcgct gattcatcta actctttggg gatgctgacc
     8101 atgaattgca tgatctggta aatgaaaaat gcttgtccaa aaaaatgtgg taaaatcagc
     8161 ggcacatacg tcccaaccaa attcagccga ttgtaaataa taaactgcgg aatcaaaatc
     8221 acttgtgcag gaatcatcat cgttactaac atgacgacga accagaatct ttttcctttg
     8281 aaatcgattc tcgataatgc gtaagcaata caagtcgagc tcagaactgt ccctatcgtc
     8341 gaaactgcag agatgacaaa cgaattccta aagaaagtaa aaaatgaaat tccagcaaac
     8401 cccgcccatc cttttgcgta attttcccat tgaaaagtct ctggaatcaa attcagcgaa
     8461 ccactcataa tctcttgatc cgttttaaaa gaacctgcga tcatccataa gaccggataa
     8521 atcatgacta gacctaatag caaaataaaa ccgtattgaa accatttcgt tactgtatta
     8581 ttcatttctt cctcctctca ttcacacaac gacccatttt tttgatgatt tgaaaatcag
     8641 aaaggtgaca gctcccataa tcaacagcat gatccacgaa atagcactag cataccccat
     8701 agaattaaaa gaaaaagctt ggttatacac ataaagagca taaaagttcg tggcatcatt
     8761 cggaccacct tgggtaatga taaatgcctg cgtaaaagtc ataaacgccg cgattgtctg
     8821 cataaccaag ttatacaaaa tgatcggcga aagaagaggt aacgtgatct tgtaaaacgt
     8881 ctgccatttg ttcgctccat cgatctctgc cgcttcataa tacgagcctg ggatctcttt
     8941 gatacctgca gcaaagatga tcattgccga accgaattgc caagcagaca tcaaaacaag
     9001 agggatcatg accagttttt gatcgctaaa ccaggaaagc gcaggtgcgc ctaatcgttc
     9061 caggaaaata ttcactaacc cgtttttaga aaacaactcc cgccaaacta gagcgataga
     9121 aatactgcca ccgatcaaac taggcacata atacaaagaa cgaaagaaag tcgttccttt
     9181 gatattcctc gttaaaagat aagcaatcaa tagaccaaag atcagtttca caggtacaga
     9241 tgcaaataca tagagaaaag tgactcggat ggagttcaaa aaacgggcat cactcagcat
     9301 ctttatataa ttatccaagc cgatccatga gactggattt cccaaacgat aatcggtgaa
     9361 cgaataataa agtgacaaga tcatcggtat cactgttatc aatagaaatc ctaggatgaa
     9421 tggagaagca aatacgtaac cgaccacatt gtcttgatcc aagaaccgtt ttagtttttc
     9481 ttttttcatg ccttcctcct gctgctatcc tagaatgttt gacgcaaact cataaagact
     9541 ctctgctgct tcttctgctg ttacttcttc ataaatgact ttttcaacta aaagattgta
     9601 ttggtcttga atttccggat tcaaagcact atcaaagaca ttcacttcgc cgttgtccat
     9661 atcggaaatc atttctaaat aatcaaatgt cgcttgcaaa gttgcatcat ctgcacgagc
     9721 gagctgttct cgaatattcg acatgattgg tacaccacgt tctcctttta aaatttcatt
     9781 ggcttcttca ctgttttgaa agaaatcaat aaacttagca gcttcttctt tatgggctga
     9841 gctttttgcc atactcagca tttgcgaaga acgtaaaggg ataccagatt ctccatctgc
     9901 ttttctgcga ggtggattaa tcaactgaat ctccatatct tcaggagccc ctgacaatat
     9961 ttctgtcaac tggttacttg atagaaacac cattgccgct tctctatccc taaccgcctg
    10021 aaaagacaga tccgtcgtac ttgcagctcc accacctggt tctggatggg cccctttgtc
    10081 taccagattt ttacgcagct ggaaaaaatc tgttagatag tctggatttt caaatcccaa
    10141 gctgttagat agatcttccc gattataaaa attcaatcct ttctcaactt gtggtatttg
    10201 aataatacag ccagctataa aatcttcgaa tcttgaaata gcatagattc ctaattcttc
    10261 agtgatttga tagctgattt gttcaaattc atcccatgtc caatttgaag ctggttcagg
    10321 gatatttaat tgttcaaata tatttttatt gtaagcaatc gcaaacgaat tcaccccgtt
    10381 agacagtcca actaattgcc cgtcgtaagt cgtcgttgct aaataggatt catctgcatt
    10441 agaaatatca attatccctt gctccacata ttcatctaat ggttcaatct gattgatgta
    10501 ttgaggaaat tgattgttca gttggaaaat gtcccagata tcatctgatg ctgctagtgt
    10561 acctaatttt gtgtaatatc cttcataatc ataaaattca taatcgatcg tcacatgggg
    10621 atttttttct tcataaagct cgattacttt tactgttgca tcgtgtcgga cttgcgaacc
    10681 ccaccaagcc atgttcagcg tgattttttt gtcatcttct tgcccacaag ccgtcaagaa
    10741 gaataacata acacttaaag ctaagatgat tctcattctt ttcttcattc tctgcacctc
    10801 tacttccatt gttatttttc aatatactgt atttttatac agaacatatt tattaaacga
    10861 tctttttcaa ggggaataaa aaccaagact tgccttatcg ttctgcctag gatgaacaac
    10921 tcaatacttt caagaaaaat tagatagtgc tgattcttct ttcatcgcta aaaacacttc
    10981 ttgcttcaat gatgggagga taattacgca aagaaaaaag gatatcagac aactgcccaa
    11041 tatccttgac atagctaaat aagcatgctt cttcgtgtgc gctcttccct aaaaaacaga
    11101 aaaataacta gtaattctgt atgaaattag gtacaatata cacgtcgatg cacaagcatt
    11161 gactatggca acttgattgg tcagttcccg ctgacttttc aagccccgac aatttagctg
    11221 ctactaaatt gtcgggtgcc ttttttagtt tttattcgtt tttcattcta tagaaagcgc
    11281 ttcaataaat caagacattt ttttgttaga atgctaataa taacaaaagg ttttaactct
    11341 tcttatgtac attacaggta aaaaaacaaa aaaatttctc ttttttttga attctgaaca
    11401 aatagtactg gagtacaaaa gacgaggaag agccactatc tgctcttcct cgtcttttga
    11461 ttctcacctg ttattcatga ttctttttct cagtttcccg atatttttcc attttacgac
    11521 taaaaagctc tccgctactt ggtggcgtat atgtgatggc atttgctcct gcttgaatgg
    11581 tctcagcaat actttcttcc gttggaccac ctgtcgcaat gataggaagt tcaggatatt
    11641 ttttacgaaa atggcgtact gcttctgcag tttctttacc tgcactgata ttgataatcg
    11701 aaataccagc tgctaacttt tcatctattt ctgtatattt cgacgttacc gtactgataa
    11761 ctggaatatc aactactcga caaacttctt ttactgtctc taaaggcgta ggaccgttca
    11821 aaacgacccc taacgaacct tgcgcttctg cgaacatgct catgtaggaa gaacgagcac
    11881 cttgtgtcaa accaccacct acaccggaga aaactggaat gtcagctgcc tcaataatgc
    11941 ttttagtaat cgctggatga ggtgtaaaag gatacacagc aattactgca ttcgcatttg
    12001 tattacgtat gatcgcaata tccgtagtaa agatgatcga acggatcaat tttccaaata
    12061 tcttgatccc gcttgcttct aaaatgattt ccggtactct tacgatatct tttctcaatt
    12121 ccgtcatgat ttctggcgta aattgttctg ccatcagcca cttctccttt gctatctccg
    12181 gtcatactga ttcaattgat acgtctcgat gacctggatg attttttgcg cataaccggg
    12241 gtctgtcgca taacctgcat cttgcaatgc ttgtgcggct tcctgatagt tggtggccgt
    12301 aatcactttt tcatacagcg caggattcca atctactcct tggacgaaca gttgtgtatg
    12361 gtcatccatg gactgttccc atgaatcata cactttgaag tctccttgga tagttatcca
    12421 ttcttcgtta acaaattcct ttgtttccag gcttactttc ttctgatccc cataagcctt
    12481 gatcccgaaa agattattgt atttgctagc caaggtactt tttccccaat ttgattctaa
    12541 tatagcttgt cctaaaatga tactaggaag cactccataa ccatcttgta gttcttgggc
    12601 atgcggaaca agccgttcaa taaattgctg atgggattgc gcttcatttt cttgctgatt
    12661 atcagtaaaa ggagaagaca gattcttcaa ggaaaaaaca aaggctaaga gtatcactgc
    12721 tacaccggca accactgctg gccaacggat attcttgcgc ttttgctttt tccacttttt
    12781 tttcgccatg atcaattcct acttttcatt taattcattc agatattctt ctagcgtcaa
    12841 ttggtgtttg gtgatatact cagcgttttc tttaccgaca taacgtaagt gccaaggttc
    12901 atatgtgatc ttagttatat cttcgtgtcc ttttggataa cgaacgataa atccatactg
    12961 cgcagcatta tctgcgatcc atttagctcc aggctgatct ccgtaacttg catccaatac
    13021 agtagaagga taactgttgt accagttttg atcgacgaca tcgactgcca gacctgtgtg
    13081 gtgttcgctg tatcctggct ccgtaatcgt ttctttagta atctttgtcg cctcctcttc
    13141 tgatacacct tgacgactca tcacatcttg gacattttgt tcgaaaactt gttgttggga
    13201 tgatacagac cggtaagctg agaccatcac taatggaaat ccagctgctt cagcagcctt
    13261 ggcaaatgct tcatattctg gagcaatgcg tttgtcgatc aagtatccgt tacttaatga
    13321 tgccaattga gtagactcat caatttcttt ttctaatttg tgatctggac caaccaaaac
    13381 tagcttccaa tcgtctgttt taacatctgg caattgtttg gacgtacgat cttttgtttc
    13441 tttgtccttt accttgctgc tggttgtttg actgttcttt tctttactag cagttgtttt
    13501 ggtgcttgtt cgttcgactg cttgattgcc ggcttgaaca tgcgaatcat catcgctaaa
    13561 aataacatag cccattgctg cgatcataat cacaactgct ccaaatccta aaaatttatt
    13621 cacttgaaaa atcctttcgt tgttcttcac gttccccatt cgcctcatga acgtaagttt
    13681 cttccagatt ttcattcacc caatcgagaa gttcgatttt ttctccttcg atctgttttt
    13741 gaacatctgc tggcagacga aaattcaaaa tatcatcata gccccattca tcatattgaa
    13801 tacttacttt caagtcaagg tatctaccag cctgctgatt atttttcaaa gggatcagct
    13861 cgacttttgc tgctttgctg ctaagccatt tttgcgcgat gcgtgttttg atttgcttgt
    13921 actctgcaac ggcaacgttt cgtttttcgg gaaaaataat cgtttgacgc aaaatctttt
    13981 gcgttagcat ccattcataa tcagtgaaaa gattttttac tttctgcatc gcttcagatg
    14041 gcagattttt ctcgccatct ttccattttt gccattgttc tggactgatc agcaagtatt
    14101 catctaaaaa atttttttca gtttcaaact gttctaataa tacactcata ataagatcaa
    14161 tatatacttg atgcacgttt atcccctcct tttaactatt ctacttaagt tttgatcaaa
    14221 aagcacctct taaatcgctg ctttcatttt ttttaatgtt tcaagtcctc tcctctttat
    14281 tcttcgtgat ttttgttata ataatagaca gacaagaaac tcaatccaac tatgaaggag
    14341 cgttttcaga tgaaaaaata tcaatgggga atcgttggtc ttggtaccat cgcacatgag
    14401 tttgccgaaa gcttcaatca agagacgagc gaactaacag cagttgcttc cagaacttca
    14461 gaaaaagcgg aaaacttcgc tcaccgttac aatattccaa aagcttacgg aagctatcaa
    14521 gaaatgttag acgatgctga aatcgacatc gtgtacattg ctgtaccaaa tagacagcat
    14581 atcgaccata tcttggcagc attggaagca ggcaaacacg ttctatgtga aaaagccatc
    14641 acgatgaata aaaaagaact ggctgacgct atgcgcttag cagaagaaaa gaatttgatc
    14701 ttagcagaag cgatgacaat cttcaacatg ccactttatc agcagttacg ctcaataatg
    14761 gatacaggaa aactgggggc tttaaaaatg atccaagcac ctttcggcag ttacaaagaa
    14821 ccagacccta aaaaccggtt cttcaatcca gaattagctg gaggagctct gttggatatc
    14881 ggtacgtatg ctgtatcttt cgctcgtttc tttttgagct ctcaacctga agtagttgcc
    14941 tctacaatgg ttccttttga aacaggcgtg gacgaacaat ccgtcactat cttgcgtaac
    15001 aaagaaaatg aacttgctgc tgtcagcttg acttttcagg caaaaatgcc aaaagtcggt
    15061 atcgttgcct ttgagaacgg ttatgtgacg attgctgatt acccgcgtgc tgatcgtgcg
    15121 gagatcttat ttacagatgg tacaaaggaa ttcatcgaaa gcgggaatac atctgaagca
    15181 atgaattacg aaataaagaa tatggtacac atgattgaag gtgacttgcc caatcgctct
    15241 ctcttcttga caaaagatgt catcgaaatc ttggatcaaa tgcaagttct atggaaaaat
    15301 atgtaatttt aaaaggttgt gaaaaagctg ttctgcatca agtaataaga acagccaaac
    15361 aaaaatagct cctcctattt ttcgtttggc tggacttatt accgcagagg aaagcttttg
    15421 aacaccgttt aagaaagagg ttgtgagcct accgttcagc tccgagtcac aaggagcaat
    15481 ttcaaaaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttgacag
    15541 gagaacaccg tttaaagaag aggttgtgaa atcagctttt tgacctgaat attagctgaa
    15601 ttcacaacct ctatttttgg attttttgtt gggagaattc tgccttgatt tcaataaaac
    15661 ttttaaaaga ctgcaaaaac tggaacaatt ctgctcgatc ctcgaattca gggcgactag
    15721 tcggcagagg catttgccga tacaatacat aaacttcttc aatacgtgtt aaaatttgtc
    15781 tgccatcatt tgactctgaa aatgtttctg cagtataaat caataaacca tagatatgtt
    15841 ttccataata agctggttgc tggattctct ctagattttc cagcatgtct ttaatcacat
    15901 tggcctgtgc tcttctcata gagaagtaag tttcgtagta tagcggctgt ctgagccagt
    15961 agttttcttg atgttctctg gcatcttctt ggctttcgcg aatgaatgtc agtaaatgtt
    16021 cgcatttgat ctgcacatct tctttatttt ctgataaaag aaattctgcc atttgtctca
    16081 aaatatttcg aaattctttt tcgatctgta tttggttttc cttcagctgc ttcgtacgat
    16141 ctggcatgta aacatttgca agcaaagcaa atactaatcc aatcgccatt aatcccgctt
    16201 cattgccgat cagctgccaa gacatgtttt cctccactaa ataatgagtg accaaaaccg
    16261 aatttactac tatcccttcg gttaatttga agcgagaggc aagcgggatg aacaggaata
    16321 aaaaaagact gaagccaaaa atagtatacc caacagcaaa aaaactgaac agtgctgttg
    16381 ccgtagctaa tataaaggct gcgattcgat tcatgcccgt acgcaatgta gagcgcttgg
    16441 tatttcctac acttaataat gcaatgatcc ctgcagctgg ccaatacaac agcgataagc
    16501 tacccgctgt gaccatcgaa aagaagacac tgatcactgt tttgactgtt cgcattccta
    16561 tacgcattta cttccccctt ttttatttta attatacttg aataatgaaa aactagctat
    16621 cagacaaaag tttaggtatg atagtaaaaa gaccaaaaag gagaatctct atgcaatttg
    16681 gccgttttcg actaggtatg cgtacgatca aaagtgcctt agctgttttt ttatgtatct
    16741 tgttttttca tttgactcaa cgaggaatcc cgatgattgc tgcattatct gctgtttttt
    16801 ctttgcgtca ggacttgact acaaccgttt cttttggaaa gtctaggatc atcggcaata
    16861 cattaggtgg tacattagct atcatttatt tttttgtcaa aagctatttt cacaatgatt
    16921 ttttggtaga attgatcttg cttcctattc tagttattat tgtgatcgtc gtgtcagatg
    16981 gtattaacaa taattccggt atcatttccg caatagctac gttgttgctg attgcgttga
    17041 gcatccctca aggcgaatcc gctttgtatg ctatccaacg tgtactagat acttttatag
    17101 ggacattcat tgcaatcggt atcaatttct ttcttcgtcc tcctgaaaca gaaaagaagg
    17161 aagaactatc agaagatttg gtcgaactcc aaaagaaaga acgattgcta agaacaaatt
    17221 tacaagagat agaagctaag atccaaaaac aaaagaaaaa tagcgaggac taattttctt
    17281 ttgcaataaa ataaaaaaat ggagagaaga actttaaaaa gtcttcctcc atttttgatt
    17341 gaacggtgaa gtgtactact ttattcttcc ttcatcgagt tttgttctgc ctcttttggc
    17401 gcttcataca tatttacttg gctagtagtc ccacgtgtcg acaaattctc tacgatttcc
    17461 ttcaagtcaa gtcctgttgt cgcttttagt gtttcttgag tagaagaaag caaatttgtg
    17521 gcataatttg tcactcggtt cgctccactg gtttctcctg aactgcttcc tgtatcgaca
    17581 acagagattt tttcgatatt tcctagcggc tgtgctgctt cgcgcatcaa ttgcggtagc
    17641 atatcgataa ccatactcaa gacagctgct tcgccatatt ccttgaaggc attggcgatc
    17701 ttttgttttg cttcggcttc tgcttttcct tttgctagtg ctgcttcggc ttctgcttgt
    17761 ccagctaaac gtgtcttatt ggcttccgct tctgccagtg cttccacacg gaatcgttct
    17821 gcttcggctt cagtcacttc tttgactttt tgagcttgtg cttcttgttc tttagcgtac
    17881 cgatcggcat ctgctttttt cttcacttcc gaatcatatt gcttttcacg acgtgtgatt
    17941 tctttttctt ccagttcgat ttgtttttga cgttctacga ttttaatctg catttcctgt
    18001 tcgattactt gttgttgtgc tcgggcactt tctaaattgt aggcttggtc tgcttttgct
    18061 ttagcaacat cttgttcttg tttgtaggtt gctaatttca gttctttttc ttttaatgat
    18121 tctgcgatct ctgtttgtcg ctgtaattca gctgcttgtg attctttttc tgcttgagct
    18181 tttttgatac gcgtttcttt tagagcttcc gcttctgcta tatctgcatc tcttttgact
    18241 tgggcgattc ttggtttccc taaagagtca agataaccgt ttttgtctct gacctctttg
    18301 atagtgaaag aaacgatgat caaccccatt tttgcaaggt ctacagaagc gacttcttgt
    18361 acgctttgac tgaacttatc gcgattttga tagatttctt ctacagtcat agaaccaaga
    18421 atcgagcgca agtgtccttc taatacttct cttgcttcat tttccagttc ttctcttgtc
    18481 ttgcctaaaa actgttctgc tgctgtagca atctcttcta cagatgaacc gattttgatg
    18541 atcgaagtac catcacacat caccggtacg ccttgttccg tatacacttc aggggtggag
    18601 acgtccaatt tactcgataa tagactgatc cgattcgaac gttgaaaaac tggcaagacg
    18661 aatgcgccac ctccacgcac aattttgatt ttgttattgc tttcatcaat gtgcacattt
    18721 ttgcttccta aataacttcc actgatgatc aacgcttcat ccggttttgc tgtctggtac
    18781 ttcgctacaa agacgatcag caacatcagt aaaatcaaag ctgcgattgc gatcgcgata
    18841 aaaacaactg gtatttccat ttaacacacg ctcctttaag cttttatcat gctttcataa
    18901 ggcatgacat aacagattct ttccttgatc tcaatgatca atactttcgt tccccgctca
    18961 gctgtctgtt cttgggggct gtaaaaacaa gctggacgac taatggaacc ggtaacactt
    19021 ttcaactgaa tttctcccat acctcgtaca gggatcggtg tgatcacaac agctacttgt
    19081 ccttccagag ttttttcaga agacgacatc gttgattcgg catgtttcat cggcatcaat
    19141 atatagaaat taagcaaaaa taccaacact atcgatatgc ctccactagc aataaataaa
    19201 aataaagatg attgatctgt taaacgctca cctagaaaac caaataagct tgtgaaagtt
    19261 aaccaaggga taataagcat cggatcaagc gggccatcaa aatcgaatac atctccaaaa
    19321 ataatcaata ggactgccaa tcctgcacaa atcagcaatg tataccaata aatcgtctca
    19381 agtgcgattc cccctatcat atacacgcct ccttccattt ctacgtctat cataccaatg
    19441 tttgaaaatt ctgtatagct tcttttaccg actcagtggt gttttttata ggaaggttgt
    19501 gtttatcgtt gacaatttgt taaatttttg ttttcatgtt aaaattttca caataaattc
    19561 tttcgaggta gcctatgttt ccttttttta agaaaaaacc attaaagctc cagaacacga
    19621 tcatccttgt cgtttgtttc tccacattcg tttctttgat tgtttctgcc atattgatca
    19681 ggaattttat tctttcgatg aaccgatcta gtgtgtcaga taaaatctct agcattgcta
    19741 aaatcgttgc caatgataaa ctcatccaaa aaaagctgca gactggtgac ccgacacatg
    19801 cgattcaagg aataacaaat gaaatcatga ttgcatcgaa tttagatttt atcgttgtca
    19861 tggaccctga aggcattcga tattctcacg cagacccaag ttacatcggt cttcctttct
    19921 caagtcatga agatgtgaaa aaagtgattg gaggacaaga atttttctct gaaaaagaag
    19981 gaatgttagg gttcggcttc cgctatttcg tccctattta ttatgatcaa gagattattg
    20041 gtgcagtttg tgttggctta acaaacaaca cgattgacaa tcaagtcaaa gatggacaaa
    20101 aaatgattgt tttttcattg attattggac ttagcgtagg catcataggc gccgtttttc
    20161 ttgcccaaaa aatgaaaaaa gtactgcttg gacttgaacc acaagaaata gcagaacagt
    20221 taagtgaaaa aacgattatc gaaaatgaag tagatgaagg aatcttagcc atctcttctg
    20281 ataaaactat cctcttgatc aatacacctg caaaaaacat tttctaccaa acagagcagc
    20341 tattggattt cgaagtagga aaaaaatcag tgataatcta tatgacgttt tgtttaaaga
    20401 gacgattgag catcaaacga ccgttcggac tcaatccatg tatttaaatg ggatagaaac
    20461 tatttctagc gttacacctc tttttagcaa tgaccgtttt ttaggagcag tcgcaacttt
    20521 acgaaatcga tcagaagtaa tgaaattaat ggaggagctt ggggggacga aacaatatat
    20581 aaactccatg agagctcaaa cccatgaatt catgaataaa cttcatgtca tttctggttt
    20641 gattgatttg aagaagtatg aagaagtcac ccattttatc cagcagctaa atcaaaatta
    20701 taaaaaagag attggccaag tctcaagtct gatcaaagta cctgcaattg ccggtttttt
    20761 attaggcaaa ataaacgaag ccaaagaaca atctgttgaa atgctgttag aaagcgattc
    20821 ttttatccct aaaatggaga tggatgactc catccattca cttttgcaag tgttagggaa
    20881 cttacttgat aatgcaaaag aagccgtgct acaaaaacat gaaagaaacg gacaggtccg
    20941 gattcattta aactatgaca cagaagctga agtattcatc gtgaccgtct gcgatgacgg
    21001 tgtcgggata tcagaagaaa tctcagaaaa aatatttacg ctaggttttt cgacgaaggg
    21061 aatcaatcgt gggtttggat tgaatctcat ccattcaatc gtcaaacgcc acggtggatt
    21121 tttagaattt tccaaacaaa caaatggcgg tacaatcgtc tatctagaat taccaaaaaa
    21181 ggaagatcaa aatgatgaca aatgtcctga taatcgaaga tgatccaatg gttgcagaat
    21241 tgaatcgtac ttttgtggaa agcatcccta atatgaacgt aatcgaaagt ttcagaaatg
    21301 tagaagaagg aaaaaaatat ttcagacaaa aaaacgcaga tctgctgctg cttgatgtat
    21361 acatgcctgg cgaaagtggt atccattttt tatcttggct tagacagcaa ggaaatgata
    21421 tcccagtgat tttgatcact gctgcagatg atatcgaaac cataaagaat gtgatccgct
    21481 acggtgcctt agattatttg atcaaaccgt tcactttgca acgttttcag ctggcgatcg
    21541 aaaagtatct tgctttctct cagacaatcc aaacgactga aaaagccgat cagaaattga
    21601 tcgatcaagt attcaaccac accactccca ttcacgaaat accatcattc atcgatttgc
    21661 ctaaaggtct gtcaaaactg actttaaaaa aaataagtgc atttattcta aaagaagata
    21721 ctattttttc aacaaaagaa ctggctcaaa aaagctcact gtcacgtata tccgtcaaga
    21781 aatatttgaa ttttttagta gaacaagata tattgaacga aagaatcaaa tatttggaaa
    21841 tcggccggcc gctaacctgt tatgaattga caaagaatgg gcggaaaaag ctcacagatt
    21901 actgttgagt catcgaatta tcccatacaa aatagtccaa gtatacagaa gtaacaactg
    21961 tttacttgga ctatttttaa acattttatt gacgaatatc catgttttct tatcttaatt
    22021 gacgggcttt aggaagagat acctcatttt tcgtttttcc agtttctatt atttcacgaa
    22081 agttctcgaa acttgtcgaa atcatatttg ataatgcctc atctgtattt gaacctacat
    22141 gcggggttac aagaactcta gggtacattt ctactaattg ctggatagca gggtctggaa
    22201 tcgtctccca tggcttgaag gatcggaaaa aaacctctgt ttcatttgca aaaacatcgg
    22261 ttgcaaaacc agctaaatga ttgcttttca aagctcttaa aatagcttca ttatcttgaa
    22321 gctctccacg ggctgtatta atcaagatgc tacctttttt catttgagct aaaaatcctt
    22381 cattgatcat ttgatcgttt tcacctggga tatgaggtac atgaatactg acaatatcgc
    22441 ttttggctaa taactcttca agcgagtgga acgtcaatac ttttttagca gcttctgtct
    22501 gatgaatatc gtagccgatc actttggctc ctagtccctt aaacaaaaca gcttccgtca
    22561 accctatcct gcctgttcct atgatcccta ctgtgcaatt tctgatttca cgactaaaca
    22621 tttgctcatc cactcgaaaa tctttgaaag atgttcggat cgtcgtatac gcagtatttc
    22681 tcaaaagcat catggcaaaa gtcaaagata actctgctat cgcatttggc gaatacgatg
    22741 gtacacgggc tactgtcata ccaaaatcag ctgccgcttg tagatcgata tgattgactc
    22801 ccacagtccg tgtaaaaaca taacgaatcc catattcctg catttttgca agattggtct
    22861 catctgctat acaatttccc ctcaacaata cagcatcatg gtcctttgct gacacgatat
    22921 tgtcatgtgt caacagttct tcataaagac tgagatcata atggtaaata ttcaattttt
    22981 caaaaaaatc tatctcgttc ggacgaacac cgtaacatgc gattctcata ctttcttcct
    23041 ccatttctta tgaaaataaa cctatgccat gaatcatttg taatacaacg atccatacag
    23101 gtgtcagtac cacagcaaaa atagttgaca gtaaggatgc attggaggct aaaagtgcct
    23161 ccctatcaaa actgatagca taggctgctg ccactgttgc agctggtgta gccatcatga
    23221 taacgattgt tgccagtgct tcaaaattga ctggtagaat attcgttgcc gtcaaaactg
    23281 ctagaatcac gatattcaat gcaggtacaa ataccacttt gactagactg taataccatg
    23341 aagttttttc agatgcagct tttttgaagc ttacttcacc taatgttgcg ccgattgcta
    23401 gccacgccaa tggtgaactt aaaccagcta aataatccat cggctgaaat agccacggtg
    23461 cagtctggtc aattcgcaaa aacgctactt ggcttatctc tccttctgca cctgtaactg
    23521 acacttgtgg cagataccct tggaacagcc aaatgaaaag gccggcaaaa gtagcgataa
    23581 tgattggatt gacgaacatc gctttgatat ttttagcttc catcttcaag ccactcattt
    23641 ttatgtatcc ataagaatac aaaaagattc ggtagccaat attgaaaatt gaggaataca
    23701 tcacgccgac aggaccataa atcgcactga cgataggcgt tccgaaaaag gtcgtagaac
    23761 caaagatcgt caggacacgc aacgttgttt gcttatcccc tttatagctt aagaaaaaag
    23821 gttttgaaac tagaataaga ataatataaa taactaatcc ccaaatcaag acattgattc
    23881 cttgcatcag taattctgta ttgatatctt gcatgaaagc tcgaaatgcc agtgctggca
    23941 aagccactgt taagacgact ttcgatagaa tttttccaac agattcagaa aaaatcccct
    24001 tttttctaca aacaaaccct aataatataa taaaaactgt tgaagtgatt gcactgacaa
    24061 tcgacatatc tgtcaaagtc gatcgaaaaa tgtcaaataa gttcatcttt ctcctcctaa
    24121 ttatgttcat ttattcacaa taaaaaaagc ttttattttg ctaatacgta ttaacatttt
    24181 taattttatc tttacattgt tacttattga acaataggaa attaaagaaa cttttgaaac
    24241 ttatgtaaag tgatt
//
LOCUS       DAZUMT010000035        23985 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000035 DAZUMT010000000
VERSION     DAZUMT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.035"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..173)
                     /locus_tag="ACG8FM_001264"
     CDS             complement(24..173)
                     /locus_tag="ACG8FM_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093937.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1141..1467
                     /locus_tag="ACG8FM_001265"
     CDS             1141..1467
                     /locus_tag="ACG8FM_001265"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5093938.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1484..2383
                     /locus_tag="ACG8FM_001266"
     CDS             1484..2383
                     /locus_tag="ACG8FM_001266"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5093939.1"
                     /translation="MIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLTVSN
                     LKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAISVP
                     QAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIAILS
                     EIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANALLRS
                     KEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPAKEE
                     KVLTQSQALDLLRKRGYTIIDSA"
     gene            3009..3263
                     /locus_tag="ACG8FM_001267"
     CDS             3009..3263
                     /locus_tag="ACG8FM_001267"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFS5093940.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3805..3963
                     /locus_tag="ACG8FM_001268"
     CDS             3805..3963
                     /locus_tag="ACG8FM_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093941.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4035..5261
                     /locus_tag="ACG8FM_001269"
     CDS             4035..5261
                     /locus_tag="ACG8FM_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5093942.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(5350..5742)
                     /gene="rpsI"
                     /locus_tag="ACG8FM_001270"
     CDS             complement(5350..5742)
                     /gene="rpsI"
                     /locus_tag="ACG8FM_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5093943.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5758..6186)
                     /gene="rplM"
                     /locus_tag="ACG8FM_001271"
     CDS             complement(5758..6186)
                     /gene="rplM"
                     /locus_tag="ACG8FM_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS5093944.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLAGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6720..7187)
                     /locus_tag="ACG8FM_001272"
     CDS             complement(6720..7187)
                     /locus_tag="ACG8FM_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5093945.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7307..8845)
                     /locus_tag="ACG8FM_001273"
     CDS             complement(7307..8845)
                     /locus_tag="ACG8FM_001273"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS5093946.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            8925..9611
                     /locus_tag="ACG8FM_001274"
     CDS             8925..9611
                     /locus_tag="ACG8FM_001274"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS5093947.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9812..10339)
                     /gene="msrA"
                     /locus_tag="ACG8FM_001275"
     CDS             complement(9812..10339)
                     /gene="msrA"
                     /locus_tag="ACG8FM_001275"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5093948.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10423..11100)
                     /gene="pgmB"
                     /locus_tag="ACG8FM_001276"
     CDS             complement(10423..11100)
                     /gene="pgmB"
                     /locus_tag="ACG8FM_001276"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5093949.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11097..12225)
                     /locus_tag="ACG8FM_001277"
                     /pseudo
     CDS             complement(11097..12225)
                     /locus_tag="ACG8FM_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12225..14408)
                     /locus_tag="ACG8FM_001278"
     CDS             complement(12225..14408)
                     /locus_tag="ACG8FM_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS5093950.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14405..15430)
                     /locus_tag="ACG8FM_001279"
     CDS             complement(14405..15430)
                     /locus_tag="ACG8FM_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5093951.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15475..16302)
                     /locus_tag="ACG8FM_001280"
     CDS             complement(15475..16302)
                     /locus_tag="ACG8FM_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5093952.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16299..17366)
                     /locus_tag="ACG8FM_001281"
     CDS             complement(16299..17366)
                     /locus_tag="ACG8FM_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS5093953.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17392..18228)
                     /locus_tag="ACG8FM_001282"
     CDS             complement(17392..18228)
                     /locus_tag="ACG8FM_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5093954.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18225..19094)
                     /locus_tag="ACG8FM_001283"
     CDS             complement(18225..19094)
                     /locus_tag="ACG8FM_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5093955.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19164..20447)
                     /locus_tag="ACG8FM_001284"
     CDS             complement(19164..20447)
                     /locus_tag="ACG8FM_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5093956.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20461..22146)
                     /locus_tag="ACG8FM_001285"
     CDS             complement(20461..22146)
                     /locus_tag="ACG8FM_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS5093957.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22421..23446
                     /locus_tag="ACG8FM_001286"
     CDS             22421..23446
                     /locus_tag="ACG8FM_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5093958.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         6972 a         4991 c         4302 g         7720 t
ORIGIN      
        1 tttacgttcc agttttattc tttctaaaaa atatactcac ttcttttctt agacttcttt
       61 ttttgattct cctctttatg gtcttgtcct tttttgcctt caaaatattt aaataaaccc
      121 ctttcaaaag catttctagg ttttggacaa taaggaatga cttttttctt catataaata
      181 cctcatttaa ctatttgata tcatcacatt atcaaaaaaa ataggttttt tattaaaact
      241 atcttgacct ttcgggagat ttcactgtga tttattttta ttcccatact tttttcgttg
      301 gtcactttcg tatacctaat ctaagcgatc atcggcgacg gtgcttttac aacgaatggc
      361 aggacaaaac aaggggcgga aaaacttttt tgcctatttc ataaaaaact ttttcctaca
      421 gcaatccctt gttttgtcct accacacgtt gtgaacccaa agcacgccga cgaacgctta
      481 gctatacgaa aatgacctcc aaaaacaaaa aagtgaggtg aatagaaaca aaaagaaatt
      541 gaaatctccc aaaatgtcgt ccgcctcgga gacaacttgc tgctgaaaat ggttggttaa
      601 ttcatttaag tattaaataa gataaatcat caaattaatc ggaaaaatag catgattggc
      661 cttatcaaaa ataaactgta aaaacttata tttctatgag tggtggaaca atgctaatac
      721 caaattaatt tatcgattac ccacctgcac aaaccaaaga tagcattatt aggaaacgaa
      781 tcaatcagcg aatccagcat tataatgagg atactaaaaa taaagttttc ataattataa
      841 atccgccttc ctacgaaagg caccaatgag gtgatgaaaa ccttagagcg ctttctttct
      901 ttttttattc cgtgttaccc tttccttgca ggtccctggt acactacaga acccgaggag
      961 gtgagtggcg gggctgtata gcggcaaccc cgcataatta tatggtgtcg gtcatccgtt
     1021 aaggcatcaa tgaatggcgc aaacccgtag cccgtaaact tatctcttcc gaagttgact
     1081 cgatttcgcc ggatgaccag tccctaccaa acctgtacta tttttttaca ggaggttgct
     1141 atgttcaaaa tctttcgtaa aaattgttgt ggcttggatg tccacaaaac gtggatctat
     1201 gcgtgtatcg gaatcacaga tgcgaatggt cgtacagtgt ataagcaagc tcgcttctct
     1261 tcctttacaa aaggtctcaa tgaattagct gaatggcttg ctaaatacaa ctgtaaggat
     1321 gtgtgtttgg aatcttccgg taaattttgg attcctgttt ttaatattct tgaaaaaact
     1381 tgttttgtca cactggctca tccgaagtac accaaacccc agaaaggaaa taaaacagat
     1441 cgcaaagatg caaaaatgga tttgtgatct ctttatgtgc gacatgatca aacccagttt
     1501 tattcctggt cttgaaatcc gccagcttcg tgatctaatg cgttatcgga cgaaactgac
     1561 caacatgaaa acaagtgaaa agaatcgtgc tttaaactgc ttaaccgttt ctaatctgaa
     1621 actagatgat gtttttagtg atgtgttcgg aaaatcatcc acttctatca tccaatactt
     1681 gttagaacat cccggtgaat catttgatgt tcgaccattt atccatgccc gttgtaaaac
     1741 tcccgttgaa gaaatccaag cggcagttga gggggcgatt tctgtacctc aagcgattaa
     1801 gcttcgccaa tgcctcaacc atattaacga gattgagaag catcgagaag agatcaatct
     1861 agagattcaa gctcttgcag aacccttctc ccctgttttg gaactacttc gtaccatacc
     1921 gggcttagac actcagccaa taacagcgat tgcgattctt tcagaaatag gaccggatat
     1981 gtccgtgttt ccaacttcga aacactttat ttcttgggct ggatgctgtc caagaaacga
     2041 tcgcagtgcc cgacgaacaa aatcaacacg tatctctcgt gcagggcagt cgttgaaacc
     2101 gctacttgta cagattgcca atgctcttct cagatccaaa gaacatccag aatttaaaaa
     2161 tcgctatcgt agaatcaaag cgaatcgtgg acacaagaaa gctatcattg ctatttgtaa
     2221 gatgctcctg accgctatct ggaatgtctt atcaaaggtg gaaccctatt ctgctaaagg
     2281 atatctggag ccaaaacctg cgaaagaaga gaaagttctg acgcaatccc aagctctgga
     2341 ccttttaaga aaacgcggat ataccattat agatagtgct taaaccaaag ttacgttgat
     2401 tcttcctaaa tagactcgtt gtcttccatt tcaaaaattt tgaaaacggt aggcttattt
     2461 agtgtaccgt tcttcaggta tcttcaaata gatttatttt caaacttatt tcctttcttc
     2521 ttttactctc ctatagctta gcatttaagt gggcactcgt gtcaccttgc tagatagcta
     2581 tgaatgccgt aataagcctc ctagtatata ggcagactgc caattataac tgattcaatc
     2641 cacattatga taaaatccac cttttcgttt catatgtgtt gaaatatcac atatgtcaaa
     2701 agtgcacgag tcttttcatt cagtgctttg taaatactca aaattattcc ttttcacttt
     2761 aaaaaaaaga taattagtac aaaatcagaa atgaggaaag tattaggatg gactagaaag
     2821 gagttgttga atttgttgca acagattggt catttatcaa ttaaaaatta ggtgaagttt
     2881 tattcatcac tgcagctgct gtttcccaga gaataaaacg tctagaagaa actggtcagt
     2941 tactgtatta tcaagcagca attgactacg aacaagcttg attactaacc aaagcgttta
     3001 tccacctgtt gattaaatca aatcaaaaat caggttttta tacgttcata aaagcaatct
     3061 ccagtgtact cgaatgtgac tgtatcatag gtccttattc gatgttaatc aaaatggggt
     3121 ttccttctac cgaatcattg gacagtttta tcaatgattt atcaaagttt ggacgaacca
     3181 acacccaaat cgttttctcg actcttgttt ccttaatggt ttatcagttc tggaatgttt
     3241 atcatgcgct tttattaaaa tagaaaagag cttttttcat gcgacaaagc agtgatcatt
     3301 tattggcaca tgcaatcttt gtggatccat tttttgaaaa tacgatattt agttttgacc
     3361 caaaaatctt taaatacgta tttgataatt tatcttaaca accagataat tttattcagc
     3421 accgtattat ctatattgaa ttctcggaat caagataaat ttttttccgt ttttgtttct
     3481 tttttaatct tcttcttgtt attaatagtg attaggatga tattcctata ttgatctttg
     3541 ataattagat ataatttttg aataacgttc cctgctattc gagctagaaa tacaagtacg
     3601 ccatgaccta gttagcgaca actacttaga gatttctttt gttctgggac aacaaaatgc
     3661 catgacaata gcagtaaata atgatacttc cgtaaggctc gccgtaaact ggggagaggt
     3721 gaaattccta tgtgcttacc caaagcggtt caaaaatttt aaactattat tttaggagat
     3781 gattttataa aatcgaaagc ctacttgaat aaaaaacaag cctgtgaatt tctaggaatt
     3841 gataataata ctttagataa gtggattcgt gtatataatt ttccagtaat aaaaattgat
     3901 ggcgtagttc gctttgacgt agaggatatc aaagatttca tgaatacatt caaaaaaaat
     3961 tgaaattttc ttctcgtcac atatactata aatagcgaaa tgtactgcct tactttttaa
     4021 aggagtgagg aagtttgcct acaaagcttt caaatgggaa atataaaact aatctgaggt
     4081 atccaaaaaa attcagagaa atcactggaa ttgcatcaga gaagtatcaa aaagtatttc
     4141 caactaggca attagctatt aaggccgaaa gggaaatgaa aaagaagatt gaaacagttt
     4201 taagagagga gaatgcaaac tcactagaat tgaaaggaaa aataaaattt aaagattttt
     4261 atgaatcaaa atggcttcct agatatgaac taggtcaaac tactaggtca aacagggctc
     4321 caagtaatgt aacaattagt aatactaagg atatattccg attgcacatc cttccaatgt
     4381 ttggtgaata tgcaatgaat tatctgaatt caaatactga gataatatca gatgaattaa
     4441 caaagaaatc gaaagaatac gcaaatataa aaattatcaa aggctatgtt cgctctatgt
     4501 ttgatattgc cgaaatacta tgttatatcg aattcaaccg aactaccaaa attattcaat
     4561 gtattaccgc gcctaagaaa atggctttag aagaaaaaag gataagggaa gggaatcaag
     4621 cattatcatc agaagagctg actgattgga tagaagcagt aaacaatgac ttaaataatc
     4681 acttgcttac acttcacgat tacacacttt tcatgcttac cttatatcta ggtgatagaa
     4741 aaagtgagac ttatgctctg caatggaaat atattgattt taataaaaat acggttcgtc
     4801 tcaaatatgc tttagataaa tatcaaagaa agacacatac aaaaggttgg aaagacaccg
     4861 ttattcaggc accagaagtt gtgatgacat tattaagaga atggaaatca gctcaggctg
     4921 aacaattgtt gaaactaaaa attatacaaa cacctgacca gtacctcttt acctattcca
     4981 aaccttctgg agaagtaaat tgtcctgttc acgctgacta cttaaactat cgaatcaatg
     5041 caataaaaaa aaggcatcct gaattggctc aattatctcc acacaaactt cgtcatacct
     5101 atgcaactat agctagggaa ggtggagcaa atatgaatca aatctctaat gctttaacgc
     5161 actcggatat ttctactaca aaaatatacg tgaacacacc tgacgtagtt gataaatctg
     5221 tttttgaagc atttcaaaaa ggattgaata agtgtgatta acatgtgttc tcaaatgtgt
     5281 tcttattata aaaaaagaca ctttccaaat aattgaaaag tgtcctgaaa tattgatata
     5341 aaaagaatat taacgttttg agaattgtga agctttacga gcttttttaa gacctggttt
     5401 tttacgttcg accatacgtg cgtcacgagt aagtaagcca gcacgtttaa gagctaaacg
     5461 gaagtcagga tcaacttcta gcaatgcacg agcgatacca tgacggatag ctcctgattg
     5521 tccagcgtat cctccgccgt ttacgttaac aaatacgtca tatgcgcctt ttgtttcagt
     5581 aacagcgaaa ggttgattga ttacttcacg caagtcagca tgtggaatgt attcttcaac
     5641 atctttttta tttacagtaa ttttgccagt tcctggtact agacgtacgc gagcaactgc
     5701 gtttttacga cggcctgtgc cgatatattg aacttgtgcc aatgaaattc cctcctatta
     5761 gattaagttt gtgatgtcta aaacttctgg ttgttgtgct gcatgtggat gttctgctcc
     5821 accgtatacg ttcaattttg taaattgttt gcggcctaaa gtgtttttag gaagcatacc
     5881 ttttacagaa gtttcgatca aacgacgaga atttttagca cgcaattcgc cagcagtgat
     5941 tgatttcaat cctcctgggt agttgctgtg acggtaataa attttgtcag tcgctttttt
     6001 acctgctaat ttcactttgt ctgcattgat gacgatgacg aaatcgccag tatccaaatg
     6061 aggtgtgaaa gttggtttat ttttaccacg tagtacagat gcaacaactg ttgaaagacg
     6121 tcccaatgga acatcagttg cgtctactac ataccattta cgttctactt cgcctggttt
     6181 ggccatatat gttgtacgca cgttattttc ctccaattta agttctgatg tttgacatac
     6241 acttcgagtt tccggggctc tttgtggggc aaacaatacc atttaacata ttacccgcta
     6301 atctagtcaa tgtcaacgtt tttctgcttg attgaaaaat ttatatgaaa ctttacttct
     6361 tttctttctt ttatataaaa gggtcgtttc ctgtcaaaat gaaaaacaaa aagaggctgt
     6421 gacaaaagtc ttgtcacaac cccttatttt gaataaacgg tgttctcctg ccaactcctc
     6481 ggtcacaagt cacaatatcc ataaaacata agaagctgtt ttttaaaatt gtttcttgtg
     6541 actcggagct gaacggcagg cttacaacct cttggtaaac ggtgttcaat cagctgaccc
     6601 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgacgttt gtttctctaa
     6661 tactcaggtc aaaacgctga tttcacaacc tctttttcgg tcaagttcaa caacagtttt
     6721 tacgcgtcga tctctggcgc tttgcccaaa tgagcttgga tcatccagat acgtttttct
     6781 gtggctgttt tgaattcgat aaagatatct tgtgttggat cgtcgccttc ttctccagaa
     6841 acttcgattc ctttttggta aagatctgct agataacgat agcctgcaac aagtgtttcc
     6901 aaacgttctg gaatagtacg atcccagcgg ccaatttcat cagggatttt tgtgttatct
     6961 gcaaattcac gcaatgtaga ataaggtgaa ccgtctaatg tgatcaagcg ttcggatact
     7021 tcatctaact gagcgttcaa atcatccatg aactcatcca tcattgggtg caatgtaagg
     7081 aaacctggtc ctctcatgta ccaatgtgtt tggtgaacaa caacagacat ttggcttaga
     7141 tctgcaacta attgatttaa tacttctttg gtttgttgga atttcatctt tcttgcctcc
     7201 taacattttc ttacacttaa aataatagta gactttcaat gaaaagtaca acaaaatcat
     7261 ttacctgaga acgattttca gttcgctttt ttatccgtaa agccaatcat ttttcagaat
     7321 cagctaattc ttctcgcagt tgagcaaatt gctgataagt ctgtgctaaa tgcttttgtg
     7381 tcgaaacaaa taatggatag attttctgat attttttatg atcagtttct tgtactccaa
     7441 cttgctcttt caattctatc aattcctgtg cttcactgat actttggatc tttccgatgc
     7501 ttctccagcc taaaatcaca gctcctaatg cggatgcttc tgtgacagta gggaaaacaa
     7561 gcgggtaacc cagtacatca gccatcatct gacgaaaaat cggagattga gcaaacccgc
     7621 ctgtagcacg gatctctgtg agctgatttt cctggcaaac tccttttaaa atccgataaa
     7681 ggttgagaca gatcccttct agaatcgcct tcatcatttc tttttctgta tgttgccgct
     7741 gtaaaccgat aaaactgcca aacgcatctg cgtcccaaag cggtgctcgc tcaccaagta
     7801 aatagggatg aaaaatcaga ccattggcac ccgctggcac aaaagcgata gccgaaagca
     7861 gtgcatcata tggattttct cctttttctt tagcagattg aacgacttcc tgtaaaaatg
     7921 tatgccctgc ccaatcaaag atgccagcac cattgctagt cgctcctccc actacccaat
     7981 gtttctgatc cagaacataa caaaacgtct ccatttctgg atggaactga ggctgagtag
     8041 taataaaacg aagtgctccg ctcgttccta cggtcaaagc ggcgatcgta tcaccaaaag
     8101 cacctaaccc tagattagaa agcggcccgt ctgcccctcc caaaacaaat gatatatcat
     8161 ttggcaaacc tagttctgct gctgtttttg atggtagacc tgttacttga taggtggaag
     8221 gaaagacttg cggcaattgc tctattcgaa tgttcaagta gtcaagcact tcttctgccc
     8281 attggaattc atggatatca aaatatccag ttcctgaagc tgttccgtag tcgctgatca
     8341 gttcgccaaa caatctataa aagatatagt ctttgatacc gacaaaataa gcagtttttt
     8401 tgaataattc aggttggctg ttttgcagcc acttgatttt gttcaatggt gtcattggat
     8461 gaatcggcgt cccagtctgc cgataaaatt ctttgccaag cacggtatat ttcagttttt
     8521 ctgcttcgtc cgctgctcgg ttatctgccc aagtgatcat tcgagataac ggtgttttct
     8581 cttcatccaa caatatcagg ccatgcatcg cagaggagaa ggagacaaat tgaatgattg
     8641 tgtctgaagg caactgttta attacttctt ttataacggt gcagacacct tgaaagatca
     8701 attcctgatc ttgttctgcc atcccttgtg tttcttggat caaagggtag cgaaaataag
     8761 cggatgctgc ttcttgggct tcatctgtga aagcaactgc tttggtttgt gtggttccga
     8821 tatctaatcc gattgaaacg ttcatttttt cacctcaaat tttatgtatg tacaaaaatt
     8881 tagtttaagc ttgcgtatct tggttttgaa aagggggaga tggcgtgtta ctttatttta
     8941 tcattggttg ctgtatcgga tcatttttat gtttaaccgc ccagcggatt ccgttaggtc
     9001 actctattat ttatcctaga tctcattgtg tcaaatgctg tcgttcgctt tcttggtatg
     9061 aattgattcc gattctctcg atcatcgtcc aacgatttcg ctgccgctat tgcaggtgtc
     9121 ggttgccctt ctactatttg ttggcagaag ccttatgtgg tggtcttttt gcctggttct
     9181 ttactttttc ttccgcaaga aatttttcga cgttcatatg gatgctttcg gctttgctat
     9241 tttcgttgat ggatttattt tacttcatgg tccattctca cacgctattc tgttcttggg
     9301 caattttatg gattttttgg ttgcaggccg gcgtcttcca atggcaaagt gtgctgttaa
     9361 cgtttatcgt aggcggtgtg tgccttcgct atggtcaatc ctatctcgga gctggcgata
     9421 ctctgttgat gctttcttgg agtggtggct tgtctctaga agagttgctt cagatcttgt
     9481 ttcttgctag tctgtttgga atatccgttt tttctgttta ctttatctgt cataaaaaaa
     9541 agttggaaaa gctgcctttc attccttttt tgagcagtgc gttgctgatt gtccttcatc
     9601 ttaggtattg aaaaaaggtt gtgacaaacg acttgtcaca acctctgata tatagtgttc
     9661 tcttaccaaa tccttgtgac ttggagctag gcgacaggtt cacaacctct ccactaaatg
     9721 gtgttcaatc agctgaccct cagtattagc tcaaaatgct ggttttacaa cctctgatat
     9781 acggtgttcc ataagtagct ttttcggctt cttatttctt gacgccccaa tgtcgacgaa
     9841 ggaaacctgc acgtccagaa ccttctcgat aaagattgta atggagcttg tttttcttgt
     9901 aataatcctg atggtaatct tctgcaggat aaaattcgct tcttggttcg atttttgtca
     9961 cgattggctt atcgaatcgc ccgctgtctt gcaatgcttg ttttgaacgc tctgcaatat
    10021 ttcgttcttc ttcatcatta tagtagatca ctggacgata agaatcaccg cgatcagcga
    10081 actgtcctaa tgcgtcagtc ggatcggttt gctgccaata gatttccacc aactgatcat
    10141 aagaaataac ttctggatca tattcgatct ctactgcttc tgtatgccct gttgtgcctg
    10201 ttgttacttg ttcataagtt ggatttggta catgcccgcc agtataaccg gatgtcacag
    10261 aaatgatccc tggctgctct tcaaatggtt tgaccataca ccaaaagcat ccgcctgcga
    10321 aaactgcttt ttttgtcata taaaatcccc cttttttctc atcatacctt gatttccaaa
    10381 aggaggataa cgttttgctt gtgacagaaa aaacggaaac tattatatac gtgatacggc
    10441 ttctgataaa tgccaaaacg aacgtacttg atcaaaaacg gttaaatcag gaatcacata
    10501 ttcagcagaa ggaagattta tgttttcacc tattcccaat acatgcatgt taccagccaa
    10561 agctgcttga caaccagctt ctgaatcttc aatgaccaag catgcatttg gcggtacatt
    10621 caattgctgt gctcctttga gaaaaacttc tggatcaggt tttgcttttg atacttgcgt
    10681 gccgtcaata aggacatcaa aataacttgt caaattcgtt ttttctaaaa tgaggcgtgc
    10741 atttttgctt gctgatccca atccgatctt gatgtttgtt tttttcaagt actcaagaac
    10801 agctattgca ccaggcaaaa cggatgtttc atccattttt tgaatcgctt gaagatatag
    10861 attgtttttt tgctcagcaa aggctgattt ctctttttct gagaatttct gttccatttt
    10921 tccaaatgac agcaatcgct ctagtgactc ggttctggat attcctttca gttgctcgtt
    10981 ttctttctca gtgaatggaa tggacagctg gttagctaaa accagccacg cttcgtaatg
    11041 ataacgagct gtgtctacaa gaacgccatc taaatcgaat agtgctgctt tgattttcat
    11101 cgattacctc cttgaaaatg aacgacttga agtaaagatg cgatgttttt agctgtgcga
    11161 tgaagattat cagtggcttg ctgtaaagct tcttgtaaag gcacagctgc aggaaggatc
    11221 ggaaaaacgg ccgaaatgcc aacatctgct gctttttcgc ttcctttacc aacacttccg
    11281 caaatagcta tcacaggaac gccctctggt actcttctag caatcccaac tggcgctttt
    11341 ccttttagac tttgtccatc tagacgtcct tcgcctacga ttacgaggtc tgcttgctgt
    11401 acccgtatcg aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga
    11461 agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat
    11521 tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc
    11581 attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa
    11641 aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt
    11701 cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt
    11761 atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt
    11821 actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat
    11881 cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc
    11941 tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg
    12001 gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa
    12061 actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata
    12121 ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc
    12181 tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt
    12241 tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt
    12301 tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga
    12361 tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata
    12421 gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt
    12481 gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca
    12541 atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc
    12601 tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa
    12661 tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg
    12721 acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt
    12781 aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg
    12841 agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta
    12901 tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct
    12961 aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg
    13021 tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg
    13081 ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat
    13141 tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc actgatatga
    13201 tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca
    13261 aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga
    13321 tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc
    13381 aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta
    13441 tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca
    13501 cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac
    13561 tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa
    13621 taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata
    13681 cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc
    13741 gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt
    13801 gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct
    13861 gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc
    13921 aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg
    13981 cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga
    14041 tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca
    14101 tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc
    14161 gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga
    14221 ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt
    14281 ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt
    14341 ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct
    14401 actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat
    14461 tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc
    14521 gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta
    14581 aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg
    14641 cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag
    14701 tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta
    14761 ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc gtaccaattt
    14821 tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg
    14881 tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga
    14941 taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa
    15001 tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct
    15061 ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt
    15121 gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag
    15181 ccaatacatg ttcgaagtga aagcgattgg ctgtacagac actcacgata tcgggagaaa
    15241 tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa
    15301 agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc
    15361 ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta
    15421 ctttcttcat ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc
    15481 tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga
    15541 ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc
    15601 ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc
    15661 tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt
    15721 caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg
    15781 cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat
    15841 atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac
    15901 tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg
    15961 tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc
    16021 tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag
    16081 acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac
    16141 tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt
    16201 accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat
    16261 gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact
    16321 tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct
    16381 gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc
    16441 atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg
    16501 tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga
    16561 ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca
    16621 tagccgatcc ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact
    16681 gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata
    16741 ggatctagcg tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga
    16801 tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg
    16861 atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata
    16921 ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga
    16981 tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt
    17041 ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg
    17101 atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt
    17161 ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga
    17221 cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg
    17281 atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt
    17341 ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt
    17401 tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat
    17461 gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt
    17521 actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat
    17581 cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat
    17641 tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc
    17701 gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa
    17761 tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata
    17821 caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat
    17881 ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa
    17941 agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac
    18001 taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata
    18061 atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga
    18121 atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa
    18181 caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc
    18241 ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct
    18301 gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata
    18361 ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt
    18421 aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc
    18481 tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc
    18541 aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg
    18601 accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc
    18661 aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa
    18721 gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt
    18781 ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg
    18841 cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta
    18901 tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc
    18961 agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca
    19021 gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc
    19081 cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg
    19141 aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga
    19201 tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt
    19261 gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca
    19321 tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct
    19381 ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc
    19441 aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc
    19501 tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca
    19561 gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa
    19621 acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca
    19681 tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa
    19741 aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa
    19801 acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca
    19861 gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt
    19921 tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg
    19981 tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct
    20041 tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata
    20101 acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc
    20161 tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta
    20221 ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca
    20281 aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg
    20341 aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga
    20401 agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc
    20461 ttattttatt attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg
    20521 actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata
    20581 acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg
    20641 aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa
    20701 ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct
    20761 taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat
    20821 ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact
    20881 ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa
    20941 gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc
    21001 gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc
    21061 ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac
    21121 cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga
    21181 tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg aacgaatcgc
    21241 ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt
    21301 cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc
    21361 tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag
    21421 gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa
    21481 tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg
    21541 atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt
    21601 cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct
    21661 tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa
    21721 atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa
    21781 ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc
    21841 ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg
    21901 tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt
    21961 tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc
    22021 ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat
    22081 catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt
    22141 atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga
    22201 aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata
    22261 agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat
    22321 gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga
    22381 attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga
    22441 cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc
    22501 aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga
    22561 accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc
    22621 tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat
    22681 ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga
    22741 agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat
    22801 tttaagctct acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat
    22861 tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa
    22921 tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg
    22981 ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca
    23041 atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg
    23101 cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc
    23161 gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg
    23221 aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct
    23281 attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac
    23341 agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc
    23401 aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa
    23461 agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc
    23521 gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg
    23581 tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt
    23641 aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca
    23701 atccatgtta tttggggata cttatctcta aaacataaat agaataacag acatgtctac
    23761 tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg
    23821 ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga
    23881 ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt
    23941 aagatccttt cccacttttc ttctagttag acaaattatt ttaga
//
LOCUS       DAZUMT010000036        23830 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000036 DAZUMT010000000
VERSION     DAZUMT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.036"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG8FM_001287"
     CDS             complement(54..788)
                     /locus_tag="ACG8FM_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5093959.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG8FM_001288"
     CDS             1115..1525
                     /locus_tag="ACG8FM_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS5093960.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG8FM_001289"
     CDS             1518..2219
                     /locus_tag="ACG8FM_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS5093961.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG8FM_001290"
     CDS             2219..3805
                     /locus_tag="ACG8FM_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093962.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG8FM_001291"
     CDS             3824..4045
                     /locus_tag="ACG8FM_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS5093963.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG8FM_001292"
     CDS             4042..4803
                     /locus_tag="ACG8FM_001292"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS5093964.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG8FM_001293"
     CDS             5183..5692
                     /locus_tag="ACG8FM_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5093965.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG8FM_001294"
     CDS             complement(5767..6306)
                     /locus_tag="ACG8FM_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS5093966.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8FM_001295"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8FM_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS5093967.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG8FM_001296"
     CDS             7319..8170
                     /locus_tag="ACG8FM_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS5093968.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG8FM_001297"
     CDS             8198..8833
                     /locus_tag="ACG8FM_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS5093969.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG8FM_001298"
     CDS             8853..9626
                     /locus_tag="ACG8FM_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5093970.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG8FM_001299"
     CDS             10034..10831
                     /locus_tag="ACG8FM_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS5093971.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG8FM_001300"
     CDS             10809..11222
                     /locus_tag="ACG8FM_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5093972.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8FM_001301"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8FM_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5093973.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8FM_001302"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8FM_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS5093974.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG8FM_001303"
     CDS             15999..16559
                     /locus_tag="ACG8FM_001303"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS5093975.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8FM_001304"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8FM_001304"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS5093976.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG8FM_001305"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG8FM_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS5093977.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8FM_001306"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8FM_001306"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS5093978.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8FM_001307"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8FM_001307"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS5093979.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8FM_001308"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8FM_001308"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS5093980.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG8FM_001309"
     CDS             21907..22485
                     /locus_tag="ACG8FM_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5093981.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG8FM_001310"
     CDS             22537..23019
                     /locus_tag="ACG8FM_001310"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS5093982.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG8FM_001311"
     CDS             23136..23510
                     /locus_tag="ACG8FM_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093983.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23623..>23830)
                     /locus_tag="ACG8FM_001312"
     CDS             complement(23623..>23830)
                     /locus_tag="ACG8FM_001312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5093984.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7781 a         4286 c         5115 g         6648 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact cctttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt
//
LOCUS       DAZUMT010000037        23672 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000037 DAZUMT010000000
VERSION     DAZUMT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.037"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACG8FM_001313"
     CDS             complement(228..1115)
                     /locus_tag="ACG8FM_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5093985.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACG8FM_001314"
     CDS             complement(1112..1981)
                     /locus_tag="ACG8FM_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5093986.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACG8FM_001315"
     CDS             complement(2118..3404)
                     /locus_tag="ACG8FM_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5093987.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACG8FM_001316"
     CDS             3677..4255
                     /locus_tag="ACG8FM_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5093988.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACG8FM_001317"
     CDS             4255..5184
                     /locus_tag="ACG8FM_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5093989.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACG8FM_001318"
     CDS             5181..6917
                     /locus_tag="ACG8FM_001318"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5093990.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACG8FM_001319"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACG8FM_001319"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS5093991.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACG8FM_001320"
     CDS             complement(7813..8322)
                     /locus_tag="ACG8FM_001320"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS5093992.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACG8FM_001321"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACG8FM_001321"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS5093993.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACG8FM_001322"
     CDS             complement(9623..10714)
                     /locus_tag="ACG8FM_001322"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS5093994.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACG8FM_001323"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACG8FM_001323"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS5093995.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACG8FM_001324"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACG8FM_001324"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS5093996.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACG8FM_001325"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACG8FM_001325"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS5093997.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14677..15651)
                     /locus_tag="ACG8FM_001326"
     CDS             complement(14677..15651)
                     /locus_tag="ACG8FM_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS5093998.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15632..17515)
                     /locus_tag="ACG8FM_001327"
     CDS             complement(15632..17515)
                     /locus_tag="ACG8FM_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS5093999.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17533..19758)
                     /locus_tag="ACG8FM_001328"
     CDS             complement(17533..19758)
                     /locus_tag="ACG8FM_001328"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5094000.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19873..20907
                     /locus_tag="ACG8FM_001329"
     CDS             19873..20907
                     /locus_tag="ACG8FM_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5094001.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20974..21987)
                     /locus_tag="ACG8FM_001330"
     CDS             complement(20974..21987)
                     /locus_tag="ACG8FM_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5094002.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22065..22253)
                     /locus_tag="ACG8FM_001331"
     CDS             complement(22065..22253)
                     /locus_tag="ACG8FM_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS5094003.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            22800..>23672
                     /locus_tag="ACG8FM_001332"
     CDS             22800..>23672
                     /locus_tag="ACG8FM_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5094004.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKKGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT         6807 a         5040 c         4286 g         7539 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt acctagtata
    14461 gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt tctgatagtt
    14521 aactttgttt ttcagaaaaa taacaattgg ttttattgaa gaaaatagtc atgaacaaaa
    14581 gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca gctttgtctt
    14641 gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa agccgatttc
    14701 atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc agctattgat
    14761 acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt ctggcggtag
    14821 ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag cggaaaaagc
    14881 aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt tatccgtatt
    14941 atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca tgccgcattc
    15001 ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac cttcgacacc
    15061 accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa aaaattttgc
    15121 tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc ctttttgtcc
    15181 ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg aaattgtcac
    15241 ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg tttcagcgta
    15301 ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct ttttattgtc
    15361 caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc acataccgga
    15421 tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa aaactggaag
    15481 tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc cgcctcttgt
    15541 ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt cgccgaagac
    15601 gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca taataatatc
    15661 ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca ttccctgaga
    15721 attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact tctctgcctg
    15781 tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag cctccttggt
    15841 acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac gaattcccca
    15901 tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca aggtagtcaa
    15961 tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa tcttcgaatg
    16021 ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact tttttgaagt
    16081 aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga gaccaaaaaa
    16141 gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca agaacagccg
    16201 cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg cccatttttt
    16261 gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct gcttggtcgc
    16321 ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg ttgatctttg
    16381 cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg cgtccttttt
    16441 tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca ttgaagtaga
    16501 tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata tactccgtta
    16561 ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc tgaaaatgcc
    16621 cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct tttaacggat
    16681 attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc agctgactgt
    16741 ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga catttgtcat
    16801 ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca actaagaaga
    16861 catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat gcagctgtac
    16921 ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt gttaaatcaa
    16981 attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc aaccaaataa
    17041 acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt agaccttctt
    17101 ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct gaaaagatcc
    17161 cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca tgtccctccc
    17221 acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca atatgactag
    17281 gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat gtttttggtc
    17341 gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac tgtttaaggg
    17401 aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta tgtgcatttg
    17461 attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct ttcatacttt
    17521 tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat caccttgaaa
    17581 atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc gcttcgaggt
    17641 ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat ggaacgctgg
    17701 ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt taggagacac
    17761 aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat aatggccata
    17821 ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt gctttttttc
    17881 ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca tagctgtttc
    17941 tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag ctgatacatg
    18001 agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt tcatacgctc
    18061 cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa acccggcatc
    18121 aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat cagttgtcaa
    18181 atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc cttgtctctg
    18241 tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc atttgatgta
    18301 gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct gccgaacatc
    18361 taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc tacctggctc
    18421 ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga tcatttcagg
    18481 ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta tttcacgtaa
    18541 tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat tgtccgcatc
    18601 tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct cctttgcttc
    18661 atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt cccagttatt
    18721 gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt cacgtagcaa
    18781 ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa ggatcatttc
    18841 aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc tgttgatccc
    18901 accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat ggttgccgga
    18961 ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat gatgaactaa
    19021 agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa atacttttgt
    19081 tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc ctggcaaagc
    19141 aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag ccgctttttc
    19201 tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga tcactgctct
    19261 atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat ccttaagtga
    19321 aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca agccttcgat
    19381 ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta agtctgatcc
    19441 attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat gagaagagta
    19501 ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa catttcctga
    19561 aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga ctggtttgcc
    19621 aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag aaagaaatgt
    19681 atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt tatctgtttt
    19741 tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta ctttttttta
    19801 taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta tttttttgag
    19861 gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt ctctcctgct
    19921 acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac tactcggcaa
    19981 cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa atatatcaaa
    20041 aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga agaacaggat
    20101 atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct gcatttaggg
    20161 atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct agatcagtta
    20221 ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt gttaaaacaa
    20281 aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga tatggtgtat
    20341 gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa tggtcataaa
    20401 agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg gaagcagatt
    20461 tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca tcacttagaa
    20521 aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt acttgcgact
    20581 aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag cgatccaatg
    20641 gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga agagattgtt
    20701 gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt gaccagtgtt
    20761 caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga gcgaattgac
    20821 ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt tagggaaagt
    20881 tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct tcgtttcttc
    20941 atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca caaaatccaa
    21001 tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt attcatgggt
    21061 aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta attctgtcaa
    21121 ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt cagttatata
    21181 cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca attgctcact
    21241 cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga aaaacttcat
    21301 aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc cgcaataaga
    21361 aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt gtttagcata
    21421 cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg cactatctcc
    21481 accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag ctaataaatc
    21541 tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag gcgctaattc
    21601 ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg caaattcttt
    21661 gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata gaacaggaac
    21721 aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat acttggactc
    21781 atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg tttttttatc
    21841 agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag gtctggggga
    21901 aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga agaaccatcc
    21961 gataaattta agaattttct ttttcataag gctccctcag tagttagtat taagcattct
    22021 gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg attttcccca
    22081 aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc aaacaaaaca
    22141 ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg atccaaaggc
    22201 agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc catttcgcca
    22261 tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt tctctaaaag
    22321 aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga cattttcttg
    22381 agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa tcgaataata
    22441 atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga aaataaaaat
    22501 ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca gaatgacgtt
    22561 cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa atcgccaaaa
    22621 taatgtagac taactaaaaa ggctctataa tttatgggac tgatgaatca gaattgattc
    22681 ttcagcccca tttttatttt aggtatcgcc gattgtaaaa ttaagctaga caactaaaaa
    22741 agtcatttgt gctacactca aaatagttcc gaccaaagaa ttataaggag tgagtacaaa
    22801 tgacctatac ccatcttaca acggatgaac ttgtaataat agagtcttat ttcaaaatta
    22861 atcaatctgt tgctaaaact gcccattgct tgaatcgttc aagacaaacg atccataaag
    22921 tatatctgtt cttcaagaaa ggaaaatcag ccttagaata ttatcaacag tataagaaaa
    22981 acaaatcaaa ctgtggtaga cgtccgcttg ttttacccga ggaacaatca gaatatattc
    23041 aaagaaaggt tgttcaagga tggacacccg atgtgattgt tggtcgtgca gcgtttccta
    23101 ttagttgttc tgctcgtacc atttatcgta tgttcaaaaa ggggctattt gattcttctg
    23161 acttaccgat gaaaggcaag cgtaaaccga atggacatca agaaagacgt ggaaaacaaa
    23221 ctttccgccg ctctattcat gaacgtgaaa aggattatag ccaattctca aatgagtttg
    23281 gtcaccttga aggtgacact atcgtaggtc tgaaacataa aagtgctgta attaccttag
    23341 ttgaacgatt atcaaaagtt atcatcacat tgaaaccgtg tggtagacaa gcgattgata
    23401 ttgaaaaaaa attaaatcaa tggtttgaat ctgtaccgaa aaacctattc aaatccatca
    23461 cttttgattg tggaaaggaa ttttcaaatt ggaaacagat cagtaatgtc aatgatattg
    23521 ccatttattt cgctgatcca ggaacgccgt ctcaaagagg cctaaacgag aattctaacg
    23581 gattgttacg tagagatggt ttattgaaat ctatggattt caattcagta gatgaatttt
    23641 ttattcaatc tgtcgcatct aaacgaaata at
//
LOCUS       DAZUMT010000038        23653 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000038 DAZUMT010000000
VERSION     DAZUMT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.038"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACG8FM_001333"
     CDS             complement(26..601)
                     /locus_tag="ACG8FM_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS5094005.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACG8FM_001334"
     CDS             complement(606..1580)
                     /locus_tag="ACG8FM_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS5094006.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACG8FM_001335"
     CDS             1863..2831
                     /locus_tag="ACG8FM_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5094007.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACG8FM_001336"
     CDS             2928..3221
                     /locus_tag="ACG8FM_001336"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5094008.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACG8FM_001337"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACG8FM_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS5094009.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACG8FM_001338"
     CDS             3678..4433
                     /locus_tag="ACG8FM_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5094010.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACG8FM_001339"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACG8FM_001339"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS5094011.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACG8FM_001340"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACG8FM_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS5094012.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACG8FM_001341"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACG8FM_001341"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS5094013.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACG8FM_001342"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACG8FM_001342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS5094014.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACG8FM_001343"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACG8FM_001343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS5094015.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACG8FM_001344"
     CDS             9170..9712
                     /locus_tag="ACG8FM_001344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS5094016.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACG8FM_001345"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACG8FM_001345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS5094017.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACG8FM_001346"
     CDS             11305..12207
                     /locus_tag="ACG8FM_001346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS5094018.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACG8FM_001347"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACG8FM_001347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS5094019.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACG8FM_001348"
     CDS             13650..14072
                     /locus_tag="ACG8FM_001348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS5094020.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACG8FM_001349"
     CDS             14541..14771
                     /locus_tag="ACG8FM_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS5094021.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACG8FM_001350"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACG8FM_001350"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5094022.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACG8FM_001351"
     CDS             16182..16358
                     /locus_tag="ACG8FM_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5094023.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACG8FM_001352"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACG8FM_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS5094024.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACG8FM_001353"
     CDS             16853..17320
                     /locus_tag="ACG8FM_001353"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5094025.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACG8FM_001354"
     CDS             17405..18289
                     /locus_tag="ACG8FM_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5094026.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACG8FM_001355"
     CDS             18403..18639
                     /locus_tag="ACG8FM_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS5094027.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACG8FM_001356"
     tRNA            18727..18807
                     /locus_tag="ACG8FM_001356"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACG8FM_001357"
     tRNA            18814..18885
                     /locus_tag="ACG8FM_001357"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACG8FM_001358"
     CDS             19011..19181
                     /locus_tag="ACG8FM_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094028.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACG8FM_001359"
     CDS             19401..20108
                     /locus_tag="ACG8FM_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5094029.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACG8FM_001360"
     CDS             complement(20167..20811)
                     /locus_tag="ACG8FM_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS5094030.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACG8FM_001361"
     CDS             20938..21225
                     /locus_tag="ACG8FM_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094031.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACG8FM_001362"
     CDS             21333..21653
                     /locus_tag="ACG8FM_001362"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5094032.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACG8FM_001363"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACG8FM_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23463
                     /locus_tag="ACG8FM_001364"
     CDS             23044..23463
                     /locus_tag="ACG8FM_001364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094033.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKRLPGIYLLSNTFI"
BASE COUNT         7820 a         4125 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaa
//
LOCUS       DAZUMT010000039        23549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000039 DAZUMT010000000
VERSION     DAZUMT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.039"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8FM_001365"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8FM_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5094034.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8FM_001366"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8FM_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5094035.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8FM_001367"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8FM_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5094036.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8FM_001368"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8FM_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094037.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8FM_001369"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8FM_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5094038.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8FM_001370"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8FM_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS5094039.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8FM_001371"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8FM_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094040.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8FM_001372"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8FM_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5094041.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACG8FM_001373"
     CDS             complement(7139..7417)
                     /locus_tag="ACG8FM_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS5094042.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACG8FM_001374"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8FM_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5094043.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8FM_001375"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8FM_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFS5094044.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8FM_001376"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8FM_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5094045.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8FM_001377"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8FM_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5094046.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8FM_001378"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8FM_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS5094047.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8FM_001379"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8FM_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5094048.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8FM_001380"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8FM_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFS5094049.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8FM_001381"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8FM_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094050.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8FM_001382"
     CDS             13967..14695
                     /locus_tag="ACG8FM_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFS5094051.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG8FM_001383"
     CDS             complement(14841..15317)
                     /locus_tag="ACG8FM_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS5094052.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG8FM_001384"
     CDS             complement(15277..15633)
                     /locus_tag="ACG8FM_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094053.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG8FM_001385"
     CDS             complement(15611..15796)
                     /locus_tag="ACG8FM_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094054.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG8FM_001386"
     CDS             complement(15797..16069)
                     /locus_tag="ACG8FM_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFS5094055.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG8FM_001387"
     CDS             complement(16066..16266)
                     /locus_tag="ACG8FM_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094056.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACG8FM_001388"
     CDS             16394..16606
                     /locus_tag="ACG8FM_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094057.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACG8FM_001389"
     CDS             complement(16639..16833)
                     /locus_tag="ACG8FM_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094058.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACG8FM_001390"
     CDS             complement(16846..17028)
                     /locus_tag="ACG8FM_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094059.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACG8FM_001391"
     CDS             complement(17025..17165)
                     /locus_tag="ACG8FM_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094060.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACG8FM_001392"
     CDS             complement(17655..18020)
                     /locus_tag="ACG8FM_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094061.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACG8FM_001393"
     CDS             complement(18060..18362)
                     /locus_tag="ACG8FM_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS5094062.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACG8FM_001394"
     CDS             complement(18362..18661)
                     /locus_tag="ACG8FM_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094063.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACG8FM_001395"
     CDS             complement(18654..18818)
                     /locus_tag="ACG8FM_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094064.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACG8FM_001396"
     CDS             complement(18824..19093)
                     /locus_tag="ACG8FM_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094065.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACG8FM_001397"
     CDS             complement(19105..19854)
                     /locus_tag="ACG8FM_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094066.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="ACG8FM_001398"
     CDS             complement(19851..20543)
                     /locus_tag="ACG8FM_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5094067.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="ACG8FM_001399"
     CDS             complement(20549..21220)
                     /locus_tag="ACG8FM_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5094068.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="ACG8FM_001400"
     CDS             complement(21213..21554)
                     /locus_tag="ACG8FM_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094069.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..22430)
                     /locus_tag="ACG8FM_001401"
     CDS             complement(21732..22430)
                     /locus_tag="ACG8FM_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS5094070.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22517..22987
                     /locus_tag="ACG8FM_001402"
     CDS             22517..22987
                     /locus_tag="ACG8FM_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094071.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23030..23209)
                     /locus_tag="ACG8FM_001403"
     CDS             complement(23030..23209)
                     /locus_tag="ACG8FM_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094072.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23196..23534)
                     /locus_tag="ACG8FM_001404"
     CDS             complement(23196..23534)
                     /locus_tag="ACG8FM_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS5094073.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT         6544 a         4964 c         3637 g         8404 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct
    22441 ctcctttttg gtataattta gttaaaaact ggtggtgttt attttgaatt tttttcactt
    22501 aacaattttt caatggatgt caataataag ttttatattg agtacgtttt taacagggat
    22561 aaaaataaaa ggaatgcgcc ctcaactaga agttgaactt aatgcttctt acttcgctgc
    22621 agatatgatt tatacaaaag taatcatttc taactattca acagaaccag ctatgttagt
    22681 taatttagaa ctttcatccg ctaacttaaa ccgcagatgg tcagctactc catttaagaa
    22741 attaattgct aaaggaggat cagtcgatga taatagaata tactctgaag ctgtaccgct
    22801 aaatattcct cctaaaagtg ctatatcttg ttaccttgca tttgaagtag ggaaaattaa
    22861 ttttaataaa ctccttaatc accaaacaaa aatgatattt actctaaata gaacccaaat
    22921 ccacaaaatt gttgatatca aaaatacaaa cttcccaata gagaaattag tgaaagaatt
    22981 aaactgagta atagtaatat acattctagt ttctcttttc tattcatttt tagtcagccc
    23041 cctcggttgg ctttttcgct ctgtactcag cttcagcttc atcaagcccc ataaaaatcc
    23101 aaaccatgta aacaatcgtt cctatcaacg cttgcctgct tccccaaagt cctaaagcgt
    23161 agatgattag cggtgcgctg aacactagcg ctctattaaa ttttcccatc cgcttgcctc
    23221 cttaagattt ccgaaaaatt tgtttctaaa aattcaagtg ttttacttct taaaaataga
    23281 tatgtgtctc ttccttcaac tggataatag acaaatccat ttttgttttt ctcgatatcg
    23341 ataatatttc tatatcttgg gttctttaaa actctagaag taaaccaatc atattttctg
    23401 ttaatccgtt ctagcacttc tggcaacgtc atccatctac cagtatcatc agcttttttt
    23461 aactcctcat aatccacttg ggagataatt acatagcctt ctggaattgg gatttttgct
    23521 tctagatatt gcattagctg ttcctccct
//
LOCUS       DAZUMT010000040        23083 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000040 DAZUMT010000000
VERSION     DAZUMT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.040"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..2680
                     /locus_tag="ACG8FM_001405"
     CDS             209..2680
                     /locus_tag="ACG8FM_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5094074.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2783..4519)
                     /locus_tag="ACG8FM_001406"
     CDS             complement(2783..4519)
                     /locus_tag="ACG8FM_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5094075.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4516..5220)
                     /locus_tag="ACG8FM_001407"
     CDS             complement(4516..5220)
                     /locus_tag="ACG8FM_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5094076.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5351..5854
                     /locus_tag="ACG8FM_001408"
     CDS             5351..5854
                     /locus_tag="ACG8FM_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS5094077.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5814..6152
                     /locus_tag="ACG8FM_001409"
     CDS             5814..6152
                     /locus_tag="ACG8FM_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS5094078.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6173..7591
                     /gene="ffh"
                     /locus_tag="ACG8FM_001410"
     CDS             6173..7591
                     /gene="ffh"
                     /locus_tag="ACG8FM_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS5094079.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7703..8281
                     /locus_tag="ACG8FM_001411"
     CDS             7703..8281
                     /locus_tag="ACG8FM_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS5094080.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8285..8806
                     /locus_tag="ACG8FM_001412"
     CDS             8285..8806
                     /locus_tag="ACG8FM_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS5094081.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8885..9439)
                     /locus_tag="ACG8FM_001413"
     CDS             complement(8885..9439)
                     /locus_tag="ACG8FM_001413"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS5094082.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9692..9967
                     /gene="rpsP"
                     /locus_tag="ACG8FM_001414"
     CDS             9692..9967
                     /gene="rpsP"
                     /locus_tag="ACG8FM_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS5094083.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9979..10230
                     /locus_tag="ACG8FM_001415"
     CDS             9979..10230
                     /locus_tag="ACG8FM_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS5094084.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10355..11146
                     /locus_tag="ACG8FM_001416"
     CDS             10355..11146
                     /locus_tag="ACG8FM_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094085.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11316..11837
                     /gene="rimM"
                     /locus_tag="ACG8FM_001417"
     CDS             11316..11837
                     /gene="rimM"
                     /locus_tag="ACG8FM_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS5094086.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11827..12588
                     /gene="trmD"
                     /locus_tag="ACG8FM_001418"
     CDS             11827..12588
                     /gene="trmD"
                     /locus_tag="ACG8FM_001418"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS5094087.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12724..13071
                     /gene="rplS"
                     /locus_tag="ACG8FM_001419"
     CDS             12724..13071
                     /gene="rplS"
                     /locus_tag="ACG8FM_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS5094088.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13444..14106
                     /locus_tag="ACG8FM_001420"
     CDS             13444..14106
                     /locus_tag="ACG8FM_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094089.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14185..17412
                     /gene="fss3"
                     /locus_tag="ACG8FM_001421"
     CDS             14185..17412
                     /gene="fss3"
                     /locus_tag="ACG8FM_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS5094090.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17526..17840
                     /locus_tag="ACG8FM_001422"
     CDS             17526..17840
                     /locus_tag="ACG8FM_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5094091.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17853..18227
                     /locus_tag="ACG8FM_001423"
     CDS             17853..18227
                     /locus_tag="ACG8FM_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5094092.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18237..18581
                     /locus_tag="ACG8FM_001424"
     CDS             18237..18581
                     /locus_tag="ACG8FM_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5094093.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18652..20004
                     /locus_tag="ACG8FM_001425"
     CDS             18652..20004
                     /locus_tag="ACG8FM_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5094094.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20064..21206
                     /locus_tag="ACG8FM_001426"
     CDS             20064..21206
                     /locus_tag="ACG8FM_001426"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS5094095.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21231..21485
                     /locus_tag="ACG8FM_001427"
     CDS             21231..21485
                     /locus_tag="ACG8FM_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094096.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21741..22925
                     /gene="mobT"
                     /locus_tag="ACG8FM_001428"
     CDS             21741..22925
                     /gene="mobT"
                     /locus_tag="ACG8FM_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5094097.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22922..23059
                     /locus_tag="ACG8FM_001429"
     CDS             22922..23059
                     /locus_tag="ACG8FM_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5094098.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7675 a         4103 c         4967 g         6338 t
ORIGIN      
        1 ttgagttgaa acgaagaaaa agtgttagta gaaaatcgcc ttcgattttc caccaacaca
       61 aaatattata gaaccctttt tttttgctat atgacaggaa tgaaaggtgt ttgcaagctt
      121 aatgtgagga acactttcca tatctgttct atactggata ggagaagttt ggaaatatcc
      181 agaaaacgaa atcaatcgag gtagccacat gaagaataaa tggataaatc gaagtaaaaa
      241 aaagaaagta acacaaagat catcagataa agtcatccgt ctttttccat cgtataaaga
      301 aggattaacg gcagatcaag tcaaggaacg aatagaaaaa ggtgcagcaa ataattcagt
      361 cgatcccaca ttcaaaacaa atcagcaaat cgtattggaa aacatattta cgtatttcaa
      421 tttgatcttt cttatcttag ccattttgct ttgtttagta gaatcctata agaacttgac
      481 ttttttacca gttatcatag ccaatacagg aatcgccatt tttcaggaaa ttcgatccaa
      541 aaagatcttg gacgaactaa atgttttaaa tacagcaaaa gtcaatgtca tacgagacgg
      601 gatagaacaa acaatagata tagaagacct tgtaatagat gacattgtta ttttagagac
      661 tggatatcaa attccagcag atgcagaggt cgtggaaggg aaactgcaag ttaatgaggc
      721 tttattgaca ggcgaagcag atgaaataac caaagaaata ggtgatccat taatgtccgg
      781 cagtttcatt gtttccggaa aagcttacgc ccgattagac aaagttggag cagattcgta
      841 tatttcccag ctgacagtca aagccaagac aatgggagaa ggagaacagt cggagatgat
      901 tgcttctctt aatcaattga tcaaatggat tgggatcatc attattccta tcggtatcct
      961 gcttttttct caaagttact tttttaatgg aaacacgatc aaagaaagtg tcgttgcaat
     1021 ggaagcagca ttgatcggta tgatacctga aggactgtac ttgctgacga cgattgcatt
     1081 agcattaagt gcgactaaac tagcaaagca aagagttctt ttacacaata tgaaaagcat
     1141 tgagacattg gcaagggtaa atgtcctttg tgtagataaa acaggaacga tcacagaaaa
     1201 taaaatgtct gttcaaaaaa tgatcgtctc taaaaaacaa gaaataatca gtcagtcaga
     1261 tcaattggaa gcatgcattg ctgactatgc aaaagcgatg gcaggagaca atgcaacaat
     1321 ggaagctgtt aaagcatatt ttacaaaaac cactgatcaa tcctatcacc atgttattcc
     1381 tttttcttct gtccaaaaat tcagcagtgt agcgttggca gacaaggtgt atgttttagg
     1441 agcgcccgaa atggttttgc gcgatcaatt gccggaatat gcggaagagt ttgtttcttt
     1501 cgctgaaaaa ggctatcgcg tgttggtctt tggtgtgtac gatgggattc ttgaagaacc
     1561 agtattgaca gaagcagtca caccattagg ctatattctg attgctaatc ctatcagaaa
     1621 agaagcaaga gccacatttg attacttcaa aaaacagcaa gtagccatca aagtgatttc
     1681 tggagataat ccgctcaccg tatccaatgt agctgtccaa gcagggattt cacatgctga
     1741 tcagtacgtt gatgcatcta aattatcaga agaagaatat gaagcagctg tcgaaaaata
     1801 tacagttttt ggacgcgtca agccagaaca aaagaaaatc ttcgttcaat tgataaagcg
     1861 taacaataca gtagctatga caggtgatgg cgtgaatgat attctagcca tgaaagaagc
     1921 agactgcagt atagcgatgg cttccggaaa tgaagccgcc atgcaggcct cacaagtggt
     1981 cttacttgat tctgactttt cacgcatgcc agaagttgtt gcagaaggac ggcgcgttgt
     2041 gaacaatatt gagcgatcag ccagcttgtt tttagtgaag aatattttct cctttttgct
     2101 ctcactgttt tcagtgatct ttgctctgac ctatccttta gaaccatcac aaatcacatt
     2161 gattagtttg tttactatcg gacttccatc tttcttgctt gctctagaag aaaacaaaaa
     2221 gcgaatcaga gggaaattca tcatgaatgt gatggaaaaa gctgtgccag gtggactaac
     2281 agatatgatc gtagtcggtg cgttggtgat ctgcggggtg actttgaatc taaataaaac
     2341 agatgtttct acagcatcta ccatgctttt gattgcagta gggttcctcg ttctctataa
     2401 aatctgttca ccgctgaata aattcagaag tcagatcatt cttttctgtg ctagcggtaa
     2461 ctttttttcg gtcatctttt tgcataagct gttttcaatc accggtatct cagctgtatc
     2521 acttttgctg ttgagcattc tatttttctc ggcagattcg atttttaggc atttgaccac
     2581 attggtagaa tatttgtttg aacgtaaaaa tgaaaaagag caaaagccgt ttagtcgcgt
     2641 agctaaaaaa tttctatcga tatttaaacg agaaaattga cgaaaagtat ctcaaaaaag
     2701 agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa
     2761 acgcacgagc agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg
     2821 ttccaagatg actgtctaca gtaatcgtac cattaaggag ttgcacgtag tcttttacaa
     2881 tcgctaaccc taatccggtc ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa
     2941 aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag
     3001 aaaacgatag atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat
     3061 attggacagc attttcaatt agatttttga taattggata aaggatatct gttttagtag
     3121 taaatggttc gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg atgattggct
     3181 gataattttg aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag
     3241 aattctcgcc atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg
     3301 catctttttg catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat
     3361 ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga
     3421 cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg
     3481 ccacccctag aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta
     3541 atgtctgccg accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca
     3601 cttgatgaat cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag
     3661 gttgatagct taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag
     3721 aatcagcatc atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt
     3781 gctcttctgt ggtagtacac gcccgataca tcttactcat ttgttcgctc aacgcattaa
     3841 tcgtctgata caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag
     3901 gattttgcac catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat
     3961 agatcagata atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga
     4021 cccaacggcg aaaactctca gttctcggta aaaaaccgct tgtcggttct gcgatacgta
     4081 aaatggcttc taactggcca ctttttttga ctggtaatgc gacatataaa agatcctcat
     4141 tcaaagtagt acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga
     4201 tttcgggacg ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct
     4261 ttccagtagc atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag
     4321 cagttttgtt ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg
     4381 ctttttttgt caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa
     4441 tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat
     4501 aaattttttt ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt
     4561 caccaagtac tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg
     4621 tacatctacg attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt
     4681 gtctcggtcg atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt
     4741 cggtgttagt tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt
     4801 gatttgtcca atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc
     4861 aattcgtctg aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt
     4921 caaatagtcg tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc
     4981 cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat
     5041 gccatccatc gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc
     5101 taattctaat ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa
     5161 attgaacgtc aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat
     5221 tcaaatctcc tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag
     5281 ggctatttta caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga
     5341 agagcagtca atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg
     5401 acagaaaaaa gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt
     5461 ctgtccagaa gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg
     5521 aaatggattc gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt
     5581 aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat
     5641 tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg
     5701 aagtttttcc ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca
     5761 aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa
     5821 tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa
     5881 aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg
     5941 aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct
     6001 tggaggatta tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat
     6061 tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat
     6121 atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt
     6181 tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt
     6241 atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga
     6301 tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc
     6361 agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt
     6421 gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt
     6481 tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa
     6541 ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc
     6601 agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat
     6661 gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa
     6721 gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat
     6781 ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga
     6841 tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat
     6901 tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat
     6961 ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt
     7021 agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac
     7081 attgattgaa aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa
     7141 aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg
     7201 tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat
     7261 cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat
     7321 gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc
     7381 tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc
     7441 aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat
     7501 gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa
     7561 aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg
     7621 aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac
     7681 tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg
     7741 gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa
     7801 attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga
     7861 agcattcgat gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac
     7921 aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc
     7981 agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga
     8041 gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga
     8101 agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt
     8161 taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga
     8221 tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata
     8281 aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa
     8341 aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc
     8401 tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa
     8461 atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc
     8521 acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa
     8581 tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc
     8641 gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg
     8701 ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac
     8761 gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata
     8821 aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg
     8881 ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga
     8941 aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga
     9001 tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat
     9061 ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt
     9121 tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata
     9181 ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca
     9241 cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat
     9301 tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt
     9361 tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa
     9421 atccaatttt ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa
     9481 aagataagta actggagcaa ataaaaacac ttactgacaa aagctacata gaagatagat
     9541 tacttatttt taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt
     9601 aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa aaactgttac actacatctt
     9661 gtgaataaaa caatggaggt ggaaataaat aatggcagtt aaaatccgtt taaaacgtat
     9721 gggttctaaa aagagtccgt tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga
     9781 tggacgtttc atcgaaactg taggtactta caatcctttg aaagaccctg cagaagtagt
     9841 tttaaaagaa gacttagttc ttgactggtt atcaaaaggt gcacaacctt ctgacactgt
     9901 acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta aattctcaaa
     9961 gaaataaggt gaccggatat gaaagattta agcgacttag tcttaacaat cgttcgtccg
    10021 ctagtcactt atcctgatca agtcaagtta gacgtcgtag agtcgaaaga cttttacgag
    10081 tacaatctaa cagtggctcc tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt
    10141 gctaaagcaa tccgcacaat cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt
    10201 ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg
    10261 cagctttttt gtttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa
    10321 gcgtatcctt ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc
    10381 atttgctcta ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct
    10441 atcaagcagt atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggcttc
    10501 caccactgaa caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt
    10561 gaaagaacaa cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct
    10621 caatatcgca gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg
    10681 tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata
    10741 tcaatatggg tttgcttcta gtcaagaaac aatccaagtt ttgcagccat ttgagatcga
    10801 cacaaatggt cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc
    10861 aggttcaagt tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa
    10921 tatagaaggc caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa
    10981 cagtctacca gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa
    11041 tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga
    11101 tcctatgtct gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga
    11161 tctgagccca tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt
    11221 ttgcataatt ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta
    11281 aaataaaaca gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa
    11341 aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc
    11401 cagaagaacg ttacaaaaaa ggaacggtgc taacactatt ccaagacgga aaagcacctg
    11461 tggaattaac agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag
    11521 gacatccttc tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag
    11581 acaactctgt agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg
    11641 tattagaaga tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg
    11701 atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg
    11761 tcgtcaaaga agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga
    11821 ttgacgatga agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg
    11881 gaatccatca tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc
    11941 cgcgattatt cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca
    12001 ggtatgctgc tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca
    12061 ccggatgtca aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa
    12121 atggcagaag aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga
    12181 tatgatgaac gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt
    12241 actgggggag aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat
    12301 gtgttaggga atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat
    12361 ccccaatata ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat
    12421 ggaaatcata aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa
    12481 cgtcggcctg atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa
    12541 gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa
    12601 tcttctttac agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa
    12661 ctattacgat attccgctgt gagaaaagct cataagaata tttggaataa ggagaagaaa
    12721 accatgaatc cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg
    12781 ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt
    12841 atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat
    12901 acagtacgta aagtttctaa cggagtgggc gttgaacgta cattcccatt gcatacacca
    12961 cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac
    13021 ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa tcagaagata aacacggaaa
    13081 acttccttgg aaatctgaaa tttgtgaaaa gtccttgtat atcaagggct ttttcttttt
    13141 tctctatcac agttttaagc catttttggc tgttttggac catttttcaa tcatattccc
    13201 ttaaaaattg tcctagattg tccgacattg actatcagtg tcgtcaaaat agggcaaaat
    13261 ttagtagtca gcttgacgac aaatataata cgaaaaatac caaaacattt accgaattta
    13321 gcaacttttg aacccaagtt tctcaaaggg tcagctatta gcaaaaaaga caaaacatgt
    13381 acttttctac taaaaagttg atgtgatagg cttctatcta aataactatt aggagttttt
    13441 atcgtgaagc aaaatgatgg acgtatcatt tggaaaaatc aaagtgagtt aaaactgatt
    13501 ttaacaatta atgaattcat cgaaaaacac ggaattacat ctagccgtca ataccagaaa
    13561 aagctatcag aaaatccaaa ttctgcacca agtatgtggt tcattaacaa aaaatatgga
    13621 tcatgggaga atttattgat tagtatagga cgagaaaata ctgggtatgg aaaatgggct
    13681 agaatgtcag aacaagaatt attggaaata gtagaagcct ttattaagtg cgagaagata
    13741 acctctcaac gaatgtacga acaaaaatca gtagggaaga atattccttc acttagtacc
    13801 attaaaaaga tgttaggaga tatacgtcca ctttttaaag aaaaaaatga tggatctcga
    13861 tttactgatt tcgaactgct gttagaacta aaaaatgaaa taatccgatt aaaactacag
    13921 gatgatttat caatgacaaa gtttcgaaaa ctagttcaat ccccaaaact accatcagtt
    13981 gatacaatta tgaaaagaac gaacaaaaat tgggaggaat tgatggcaga gattggattt
    14041 gattatcgaa gaatcaaaat ttataagcag agaaacaacc tatccaagac aaagaaaact
    14101 aaatagtatc actctaaatc agacgttcaa aattaaaatt tggacgtctt ttttattacc
    14161 caaaaacgaa aggagattta ttttatgaaa aagccaagat ttaagaattg gcgtttgctc
    14221 gcaacactga gtctattatg ccaaacaatt ggtggatcgc ttggcccaac gattgccttt
    14281 gctgatgaaa ttactcatcc acaaacggta actgtggaat tggatttggc tcaccagtat
    14341 gctgtcgaag gtacatttag cgatggtcgt cccatgtcag aagttaccgt tccgcactat
    14401 gcagtttaca atggtgtgaa gcaagatatt ttctgtattg agccaggggt gccgatttac
    14461 aatgagttca ctcctggtta tgagaaaaat ccattgcctg atatgtcgga gaaagcgaaa
    14521 ttagtttccg ttttatggaa ggaagctggt acggatgtgg atacacatat tgtggcccag
    14581 aagatgattt ggcaagaagt taatggctac acgcttcact caattaaacg ttctgatggt
    14641 agtgcagtaa atatcgcagc cattgagtca aaaatcaatc aagccattgc ggattatcaa
    14701 aagaaaccaa gcttccataa ctccacagtg aaaaccgtgt taggtcaatc gactactgtg
    14761 acagatacga acggcttgaa tttatcggag tttgatgaag ttgtcgagaa tatcgcaaat
    14821 attgattatc gtgtgaacgg caatcaacta gtgattactc caaatgccaa ttcaaaagag
    14881 agtggtgaat taaccctaaa gaagtcagct aatacaggaa caccggtagc ttataaaatg
    14941 gctggtcaac aaactttgat ggcgggggca attgataagc ccaataccta cacaatcaaa
    15001 attgatgtgg aaactgaggg ttctttgaag attaaaaaag tcgataaaga aacaggaaat
    15061 gttgtaccgg gaaccgtttt ccatttagac tttggaaaaa cattacctgc aaaagacgtg
    15121 accaccgata aagaaggcat tgcgacattg gatgagattc ctcatggcgc gaaagtaacc
    15181 attactgaaa aatcagtgcc agctccttat acgattgaca ctacaccaat caccgcaacc
    15241 attaaatctg gtgagactat ttctgtgact tcaaaaaata ctcgcgaaaa aggtcaaatc
    15301 attttgaata aaatgggggt ggaaacagga actgatcttt ggaacgacaa atactcatta
    15361 gccggaaata cgtttgccat tcgcaaagac agtccaaccg gtgaaattgt ccaagaaatg
    15421 accacggatg aaaaaggtca tgcggaaaca ccaaaagaga ttgccaatgc gttggaattg
    15481 ggaacctatt atgtgaccga aaccaaagct agtaatggct ttgtgaatac ctttaaacca
    15541 gtaaaagtcg aattgaacta tgtcaatcaa accgtggctc ttgttaccag taacgtaaaa
    15601 gggcaaaacc aagaaatcac tggtgaaacc actttgacaa aagaagacaa agataccggt
    15661 aatgaaactc aaggtaaagc tgagtttaaa ggagctgaat atactctctt tactgcaaaa
    15721 gatggtcaag cggtcaaatg gagtgaggct tttaaaccag agttagtgaa gggaacgaaa
    15781 gcttctgatg aaacggtgac tttgacttta gatgaaaaga atcaagttgc tgttaaacac
    15841 ttagcgatta atgagtatta ctggcaagaa accaaagcac ctgaaggata taccttggat
    15901 gaaacaaagt accctgtttc catcaaaaag gtggatgata acgaaaaaaa tgccgtgatt
    15961 acccgagatg ttacggcaaa agaacaagtc attcgctttg gctttgattt ctttaaattt
    16021 gctggatcag ctgctgggac tgccgaaact ggcttcaatg atctgacctt taaagtatcg
    16081 ccattggaag gaaccagtga gattacaggt gccgaagatg aagcaattac agcttataac
    16141 gagcaactag gttttgatgg ctatggcaag tttgaaaatc ttccttatgg ggattattta
    16201 cttgaagaag tagaggctcc agaaggattt caaaaaatta cgccattaga aatccgttct
    16261 acttttaaag aaaataaagc ggattacact aagagtgaat acatttttac gattaccgaa
    16321 aaaggacaaa aacaaccaat taaaacggtg accgttcctt acgagaaact gacgaataaa
    16381 gcattttctg ttagcttgaa tcgtttgatg ctctatgatt tacctgaaga agaagatagc
    16441 ttaacttctc ttgggacttg gaaagacgga ggcaaagaat tgacagacct tgattctacc
    16501 gagctagttg ataaattgag ctataacttg catgaaatca aagaagactg gtatgtggta
    16561 gcgcaagcca ttgatgtgga agcgacaaaa gctgcccaag aaaaagatga aaaagccaaa
    16621 ccagtggtaa ttgccgaaac aaccgcaacg ttggcgaata aagagaaaac aggaacttgg
    16681 aaaattttgc ataaattaac cgcagaacaa gttttggata aaaccatcgt cttattcaac
    16741 tatgtgtatg aaaacaagaa accctatgaa gcaggtgatg atccagtagc gaaagatgct
    16801 agcttgaaca atcaagccca aactgtcaat tgtacggtgg aacgccatgt ttccatccaa
    16861 acaaaagccc acctagaaga tggttcgcaa acctttactc atggggatgt ggtggatatg
    16921 ttcgatgatg tgtcgattac ccatgatgta ctggatggct caaaagaagc ttttgaaaca
    16981 attctgtatg cattacttcc ggataatacg accaaagaaa tttggaaatc tggcaaaatt
    17041 gattatgaag tgaatgacaa agaattcact aaaaccgtac ttgcggaaaa agtggacaca
    17101 agtaaatatc cagaaggtac aagttttacc ttcaaagaaa ttaactatgg caaagacgga
    17161 aacatcaatg ggaaacacaa tgaggatcta aaagaaaaaa ctcaaacttt aacacctaaa
    17221 aaagttccga caacaccaag tgcaccagaa caaccggaaa taccaactgt tccaagtgac
    17281 tctcaagaat ctagtcctac agtaaagaca ttcccacaaa caggggagaa aaattctaat
    17341 gttctgctgt ttattgggtt taccttgatc tttgcgactg ctgggtatta tttctggaat
    17401 cgccgaaact aaggtgatgt gatgaaacaa ctaaatagga aaaaccaacg tgcgcgaccg
    17461 cctcctaaaa aaagtggtcg cgctattttt gtatccaaaa ctaaaaatga aaagaggaaa
    17521 agaagatgga attgaaattt gttgtgccag atatggccga aacctttgga aagataaggt
    17581 atgccggcga aggcgaagtt ttaactgaag gatatgggcg gaatactaca gtaattggtc
    17641 gtagttatca tttgtattcc agtaagcaac gagccgatga tattgaagtg gtggtagctg
    17701 cggaagccgg cgaaaaggat tttgaccaag atcaaccact aaaagccgtg aatccccatt
    17761 tggttgccaa aggctatgag attgagaatc gtgggtttac cgactacgtg ttgtatgtcg
    17821 atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt attgttgtag
    17881 attcaggact aacgtttggg aaattacgat tttccgcatt acgccgagaa gtacggaagc
    17941 aaaatgagga tggaacggtt agtaacgagg taaaagaacg gacttataat ttgaaatcct
    18001 ctgcgcaagg tcgaatgatc caagttagta tcccagcaaa tgtaccctta cgcgagtttg
    18061 cctatgacgc agaggtagag ttggtcaatc caatcatgga tacggtcgcc aattatgttt
    18121 tccgagaagg aactactgtc aattggttca ttaaggccga tgatttagtc ttgaaacgac
    18181 aaccagatca gggaaattct acaaaccaaa atgaaggaaa gaaataggtg aagcgtatgg
    18241 aaattaatgt tgaacatatt ttagattgtc tcgatcaata tggaaaaggt gagctaacgg
    18301 aggaacaact cactaaagca ttaacctatg atgaaaaaat gtttctgatt atgcatcaag
    18361 ggttactgga agtaggtaac gatgtcgaag aagattttga tgtactgaat tggttagggg
    18421 aagaagagtc cttctttatg gttatcgaag taaatgaaag tctatgtcga gaagccgaaa
    18481 ccgtattgga agaaattggt gtggaaatgc cggatgccat tgaagttttt ttaaagcaac
    18541 tagtggaaac gaaacaactc cctatagcag taaatgacta gcataactgc ttgaaattgg
    18601 atcaatgaaa tcctatgaag cttttttcga ttggaggtgg aaaccataaa aatgtacaaa
    18661 gggcatcgta tacgggcagg agatcagcac ttggtttatc actttgttct tggctggcta
    18721 ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagcttag acaatttgat
    18781 atttctaagc tatcactttc tactatagaa atcgttcgtt ctataaaaga tttaatctat
    18841 ttgctaggaa gtctcgttct ttcaggaagc acgatgttgc tttatataca cttttttcag
    18901 gatcattggc gcagtttgtg gcatcgacaa aagctagccc ggatgattct agaaaatcat
    18961 tggtacgaag taaaacaaac tcagagtgaa ggatttttca aagacctcaa tagtagtcga
    19021 accaaagaga caatcagtta cttccccaaa atctattacc gtatgaagga tggtttactt
    19081 tctattcgcg ttcaaatttc actgggaaaa tatcaagatc agcttttaaa gctggaaaag
    19141 aagttagaaa gtggtctata ttgtgaacta gtagaaaagg aactcaaaga ctcctatgtg
    19201 gagtacactt tactgtatga tataattgcc aatcgaattg ggatagacga agtagtggca
    19261 gaaagtgggg ctttgcgatt gatgaaaaat caagtatggg cttatgattc tttacctcat
    19321 atgttaatcg ctggcggcac aggtggtggg aagacttact ttttactcac tatcatcgag
    19381 gctttattaa aatctgatgc cgagcttttt gttcttgatc ccaaaaatgc ggacttagcc
    19441 gatttaggta cggtaatgcc tcatgtctat tcccaaaagg aggaaatttc tgcttgtgtg
    19501 gaagatttct atgagcgcat gatgactcgt agcaaggcaa tgaaagaaat gcccaactac
    19561 aaaacaggag agaattatgc gtttcttgga cttccaccaa actttttaat ctttgatgaa
    19621 tacgtggttt atatggaaat gttaacgacg aaggaaagtg cggtgatttt gaataagctc
    19681 aaacaaatcg tgatgttagg tcgtcaatct ggtttctttc tgattctagc ttgtcaaaga
    19741 ccggatgcga aatatttagg agacgggatt cgcgatcaat ttaatttccg ggtggctttg
    19801 ggtcgtatga gtgaactggg atattccatg atgtttggcg aggttgataa aaaattcttt
    19861 atgaagcgta tcaaaggtcg aggctatgta gatacgggag ggagtgtgat tagtgagttt
    19921 tatacgccac ttgtaccaaa aggatatgat ttcttaaggg aaattagcga cattggtgga
    19981 ttaaacgtta atacggaagg ataaaataat agtatgtagc ctaggagtgt caagaatcct
    20041 gggctttttt tacgaggtga tttatgacat ttttagattt attcgctggg attggtggct
    20101 tttgcttagg tatggaacaa gcgggtcacc agtgtatcgg cttttgtgaa attgatgatt
    20161 ttgctcgcca gagttataag gcaatccatg atacaagtaa ggaggtggaa atgcatgaca
    20221 tcacaagtgt atcagacgag tttattcaat ctctcggacc agtggatatc ctttgcggtg
    20281 gatttccgtg ccaagctttt tcaattgcgg gaaagcggca aggattttcc gatactcgag
    20341 gtactttatt ctttgaaatc gctcggttcg ccgctattct ccaacctaag tttttattcc
    20401 ttgagaacgt ccggggatta ctcaatcacg aaggaggggc tacgttcgag acgatcctcc
    20461 gaacgctgga tggattgggg tatgatgtgg aatggcaagt gcttaactca aaggcctacg
    20521 ttccccaaaa ccgtgagcgg atcttcctta tcggacattc tcgagacgca tgtaccgaac
    20581 aagtatttcc tatcattgga tcgtctccaa catctgatca aaacatcaga aacttattaa
    20641 atatcaaccc ttccaatcga ggaatggggg gacaagtcta tgggtcagat gacgtagcgc
    20701 ctaccttgac cggtgatgaa ggaatcaaaa tcgcattgcc ggtagatgat ggaatttctg
    20761 tagcgggtat gttactggga aattttgaac aaggcaatcg ggtttatgag ataactggaa
    20821 cagcgcctac tttgtcaacg aaacaaggcg gaacgaagat catgcttcgg aaaaacgaaa
    20881 cagaatatca agaggtgaag ccagggaaca gtgtgaacct ggcttttcct aattccacaa
    20941 gtcgtcgagg ccgtctggga aaacaaagtg tgcatacctt attaacagga gaccaacaag
    21001 cagtcgtcac ggatcagtat caaattcgaa agttaacacc tagggagtgc tggcggcttc
    21061 agggctttcc ggactgggct tttgatcgag cttctcaagt caattcagat agtcagttgt
    21121 ataagcaggc tggaaattct gtaacagttc cggtcatttt tgatattgct agaagattaa
    21181 aggaggtaaa aacagatgat ttatgatgag ttgattgggg aatctactgg gtgattggca
    21241 agattcagtc agatccggaa ttggaagaag aactgtaccg gctcaatttt gatattcgca
    21301 aaaacggggt gaaagtccct gatgatccgt atgtaattga tgaggaaacg gatgctcgaa
    21361 tggagctgaa tcaagtcatc agtgaattgg aacgaattgc ggactttgct aaagaacctg
    21421 atatccgcca atacttgttt gagataaaag cagagttgga aattgagggt attaccgcgg
    21481 agtaacggcc aaataaacca ctgataggca ctgaccgcgg gggatttgcg aagcagaaat
    21541 cctccgtggt gctgtgcctg tttatggacg gcgttagccg gccatgatgt ctcacccccc
    21601 gtatctaaca ggggggtaca aaaacacaaa aaataagcaa gcaacgataa ggaatggctg
    21661 tcctatcaag gataataagc aatttgtgta gatgttaact gatttgaaaa agttaacacc
    21721 ttaaatttgg aggtgagaac ttggcacaaa ggaatttaga ttatcggcta ttaaaagacc
    21781 ggcgtaatga atatggcgtt tcacaaaata aattagctac gacttgtgga cttagtcgcc
    21841 cgtacttaaa tcagatagaa aatggtggcg taaccgcatc gacaaaaacc atgaggaaaa
    21901 tttttgacca actggaaagt ttcaatcccg agttaccttt aacgttactg tttgactatg
    21961 taaggattcg ctttcccaca acggatgcga ggaaaatcat tcaagagatt ctccatttga
    22021 aatttgatta tatgctccac gaagattacg ccttttactc ctatcaagaa caatatgtta
    22081 tgggagatat tgtggtgatg ttgtcacatg aagaagataa aggcgtcctt ttagaattaa
    22141 agggtcgtgg ttgtcggcag tttgaaactt ttttactcgc ccaaaagcgt agctggtatg
    22201 actttttcga agattgtcta aaagccggtg gcgtgatgaa acgcttagac ctggcaatca
    22261 atgatcgagt tggcctgtta gatattcctg atttaacgaa aaaatgtcag aaggaagaat
    22321 gtatctcctt gtttcgtacc ttcaaaagct atcgttccgg ggaactgttg aaacctgatg
    22381 ataaagatgg tatgggcaat actttgtata ttggcagtct taagagtgag gtctatttct
    22441 gtttgtacga aaaagactat gagcaatata tcaagttagg gattccttta gaccagaccg
    22501 aaacgaaaaa tcgttttgaa atccgactga aaaatgatcg agcttatcat gcaattcaag
    22561 atttgttaaa aggtcgcagt attgaaagta ccacattttc cattattaac cggtacttgc
    22621 gatttgcgga taaggtggaa ggcaaaaggc gaactaactg gcccttgaat gaacaatggg
    22681 ggcgcttcat tggacggaat cagaaggaaa ttcaattaac ttctgaaccg aagccttata
    22741 ccattgaacg aacgttgaac tggcttggac gacaagtcgc ccctacgtgg aagatggcca
    22801 aggagttgga tcggttgaat cagacaacgt acattcagga tatggtaaat aatactcgac
    22861 tatcagaccg gcacaaaaag attttggagc aacaaagtat ggcaattgaa aatttgatta
    22921 tatgaaaaga ggaatcgtga tggaaattgt gaaagatatt ttattggtgt ttggtggcag
    22981 tatgcttggt gttactgtga tgtgcttgat gcatgcaagt gcagcggcgg accgagcaat
    23041 ggaaaagaaa ggaaagtgat gaaatgaatt tcggtcaaaa ttt
//
LOCUS       DAZUMT010000041        22823 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000041 DAZUMT010000000
VERSION     DAZUMT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.041"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG8FM_001430"
     CDS             3..371
                     /locus_tag="ACG8FM_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS5094099.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG8FM_001431"
     CDS             551..1492
                     /locus_tag="ACG8FM_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS5094100.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG8FM_001432"
     CDS             1561..2292
                     /locus_tag="ACG8FM_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5094101.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG8FM_001433"
     CDS             2436..4616
                     /locus_tag="ACG8FM_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS5094102.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG8FM_001434"
     CDS             4636..5118
                     /locus_tag="ACG8FM_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS5094103.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG8FM_001435"
     CDS             5134..5895
                     /locus_tag="ACG8FM_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5094104.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG8FM_001436"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG8FM_001436"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS5094105.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG8FM_001437"
     CDS             7667..8152
                     /locus_tag="ACG8FM_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS5094106.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG8FM_001438"
     CDS             8145..8828
                     /locus_tag="ACG8FM_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS5094107.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG8FM_001439"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG8FM_001439"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS5094108.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG8FM_001440"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG8FM_001440"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS5094109.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG8FM_001441"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG8FM_001441"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS5094110.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG8FM_001442"
     CDS             13125..13457
                     /locus_tag="ACG8FM_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5094111.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG8FM_001443"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG8FM_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS5094112.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG8FM_001444"
     CDS             14283..15656
                     /locus_tag="ACG8FM_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5094113.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG8FM_001445"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG8FM_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS5094114.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG8FM_001446"
     CDS             16711..17151
                     /locus_tag="ACG8FM_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS5094115.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACG8FM_001447"
     CDS             17295..17996
                     /locus_tag="ACG8FM_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS5094116.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACG8FM_001448"
     CDS             17993..19114
                     /locus_tag="ACG8FM_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS5094117.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACG8FM_001449"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACG8FM_001449"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS5094118.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACG8FM_001450"
     CDS             20493..21401
                     /locus_tag="ACG8FM_001450"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5094119.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACG8FM_001451"
     CDS             21436..21708
                     /locus_tag="ACG8FM_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS5094120.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACG8FM_001452"
     CDS             21719..22765
                     /locus_tag="ACG8FM_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS5094121.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7696 a         3980 c         5011 g         6136 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ata
//
LOCUS       DAZUMT010000042        22720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000042 DAZUMT010000000
VERSION     DAZUMT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.042"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..499)
                     /locus_tag="ACG8FM_001453"
     CDS             complement(122..499)
                     /locus_tag="ACG8FM_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094122.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /gene="purC"
                     /locus_tag="ACG8FM_001454"
     CDS             755..1483
                     /gene="purC"
                     /locus_tag="ACG8FM_001454"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS5094123.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="ACG8FM_001455"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="ACG8FM_001455"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS5094124.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="ACG8FM_001456"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="ACG8FM_001456"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS5094125.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="ACG8FM_001457"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="ACG8FM_001457"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS5094126.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /gene="purF"
                     /locus_tag="ACG8FM_001458"
     CDS             4618..6057
                     /gene="purF"
                     /locus_tag="ACG8FM_001458"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS5094127.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /gene="purM"
                     /locus_tag="ACG8FM_001459"
     CDS             6080..7132
                     /gene="purM"
                     /locus_tag="ACG8FM_001459"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS5094128.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="ACG8FM_001460"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="ACG8FM_001460"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS5094129.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="ACG8FM_001461"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="ACG8FM_001461"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS5094130.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="ACG8FM_001462"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="ACG8FM_001462"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS5094131.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="ACG8FM_001463"
     CDS             10651..11565
                     /locus_tag="ACG8FM_001463"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS5094132.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="ACG8FM_001464"
     CDS             complement(11698..13263)
                     /locus_tag="ACG8FM_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094133.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="ACG8FM_001465"
     CDS             complement(13256..14707)
                     /locus_tag="ACG8FM_001465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5094134.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="ACG8FM_001466"
     CDS             complement(14721..15737)
                     /locus_tag="ACG8FM_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5094135.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /gene="chvE"
                     /locus_tag="ACG8FM_001467"
     CDS             15957..17045
                     /gene="chvE"
                     /locus_tag="ACG8FM_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5094136.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /gene="mmsA"
                     /locus_tag="ACG8FM_001468"
     CDS             17099..18625
                     /gene="mmsA"
                     /locus_tag="ACG8FM_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5094137.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /gene="mmsB"
                     /locus_tag="ACG8FM_001469"
     CDS             18627..19832
                     /gene="mmsB"
                     /locus_tag="ACG8FM_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS5094138.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="ACG8FM_001470"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="ACG8FM_001470"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS5094139.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..>22720
                     /locus_tag="ACG8FM_001471"
     CDS             21330..>22720
                     /locus_tag="ACG8FM_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5094140.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYS"
BASE COUNT         7307 a         3837 c         5148 g         6428 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa
//
LOCUS       DAZUMT010000043        22645 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000043 DAZUMT010000000
VERSION     DAZUMT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.043"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..846
                     /locus_tag="ACG8FM_001472"
     CDS             34..846
                     /locus_tag="ACG8FM_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS5094141.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            843..1643
                     /locus_tag="ACG8FM_001473"
     CDS             843..1643
                     /locus_tag="ACG8FM_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS5094142.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1804..3072
                     /gene="rseP"
                     /locus_tag="ACG8FM_001474"
     CDS             1804..3072
                     /gene="rseP"
                     /locus_tag="ACG8FM_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS5094143.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3140..4849
                     /locus_tag="ACG8FM_001475"
     CDS             3140..4849
                     /locus_tag="ACG8FM_001475"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS5094144.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5061..9419
                     /locus_tag="ACG8FM_001476"
     CDS             5061..9419
                     /locus_tag="ACG8FM_001476"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS5094145.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9564..10037
                     /gene="rimP"
                     /locus_tag="ACG8FM_001477"
     CDS             9564..10037
                     /gene="rimP"
                     /locus_tag="ACG8FM_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS5094146.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10060..11235
                     /gene="nusA"
                     /locus_tag="ACG8FM_001478"
     CDS             10060..11235
                     /gene="nusA"
                     /locus_tag="ACG8FM_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS5094147.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11257..11550
                     /locus_tag="ACG8FM_001479"
     CDS             11257..11550
                     /locus_tag="ACG8FM_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS5094148.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11547..11858
                     /locus_tag="ACG8FM_001480"
     CDS             11547..11858
                     /locus_tag="ACG8FM_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS5094149.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11871..14177
                     /gene="infB"
                     /locus_tag="ACG8FM_001481"
     CDS             11871..14177
                     /gene="infB"
                     /locus_tag="ACG8FM_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS5094150.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14204..14551
                     /gene="rbfA"
                     /locus_tag="ACG8FM_001482"
     CDS             14204..14551
                     /gene="rbfA"
                     /locus_tag="ACG8FM_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS5094151.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14708..15164)
                     /locus_tag="ACG8FM_001483"
                     /pseudo
     CDS             complement(14708..15164)
                     /locus_tag="ACG8FM_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15329..17691
                     /locus_tag="ACG8FM_001484"
                     /pseudo
     CDS             15329..17691
                     /locus_tag="ACG8FM_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18059..18982
                     /gene="truB"
                     /locus_tag="ACG8FM_001485"
     CDS             18059..18982
                     /gene="truB"
                     /locus_tag="ACG8FM_001485"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS5094152.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18986..19927
                     /gene="ribF"
                     /locus_tag="ACG8FM_001486"
     CDS             18986..19927
                     /gene="ribF"
                     /locus_tag="ACG8FM_001486"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS5094153.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20279..21064
                     /locus_tag="ACG8FM_001487"
     CDS             20279..21064
                     /locus_tag="ACG8FM_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5094154.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21077..21928
                     /locus_tag="ACG8FM_001488"
     CDS             21077..21928
                     /locus_tag="ACG8FM_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5094155.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22052..22504)
                     /locus_tag="ACG8FM_001489"
     CDS             complement(22052..22504)
                     /locus_tag="ACG8FM_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094156.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7683 a         3956 c         4955 g         6051 t
ORIGIN      
        1 agtgggaatc ttttaagtat aggaggaaaa agaatgttac gtttttttcc gcaaaaaaac
       61 aaatatattc aagaaaaaag cgaatttcaa tttaataccg atggaccaat tcctcagcac
      121 attgccatca tcatggacgg caatggtcgt tgggcgcaaa accgtcgttt gccacgagtt
      181 gctggccata aagaaggcat ggaaacagta aagaaagtca caaaaaaagc atcaaggctt
      241 ggagtgaaag tgttgacgct ttatgccttt tcgactgaaa actggaagcg accaaaagat
      301 gaagtaagtt ttctcatgca actacctgtc gacttctttg atacgttcgt accagaattg
      361 attaaagaaa atgtcaaagt tcatgtcatg ggttatgaaa acgtattgcc agaacatacg
      421 caagatgctg taagacgtgc aattgagcaa acaaaaaaca atactggaat ggtattgaat
      481 tttgctttga attatggaag cagagcagag atcgtgactg ctgtccaaga aattgccgaa
      541 gaagtagcaa aaggagaaat acacgcagaa gaaatcgatg atgaattgat tgctaagcat
      601 ttaatgacag gtttcttgcc taaagagtta caagatcctg agctgatgat ccgtacaagt
      661 ggagaagagc gaattagtaa ttttctgtta tggcagatcg catatagtga gctgtatttt
      721 acaaaagcgc tttggcctga ttttgatggt gcgcatcttg aagaagcaat cgcctcttat
      781 caaaatcgag accgtcgatt cggcggtgta aaaaaaacag aagatgagaa aggggatcag
      841 tcatgaaaca acgtgtggtc acagcagccg tggcgctagt cttgtttatt ccgattatat
      901 ggatcggagg catagcggtt gagcttacag cagcaatctt agcagtaata ggagtttacg
      961 agctgtttcg gatgaaagga ttgacactct taagttttga aggagtttta tccgccgttg
     1021 gtgcagtgtt acttgttttg ccaaaagaaa gatggttttt ctttcttcca gagaatgtaa
     1081 gtacatttat gttattttat cttatagtta tgattctttt aggaacatca gttatttcta
     1141 aaaatacgta tacgattgat gaagcaggtt ttcctgtgct tgtcagcttg tatgtgggaa
     1201 taggtttcca gaattttgtc aatgcacgaa cagaaagctt ggctgttttg atatttggtc
     1261 tatttattgt ttgggcaact gatatcggag catatatggt tggtcgacgt tatggtcagc
     1321 ataagttaag tcctgatatt tcgcccaaca aaacgattga aggtgcgctc ggtggtattg
     1381 gtagtgctgt tgtagtcgca tttctttatt tattgctata cccagcaaaa gaactattta
     1441 atcatggtat gtttgtcatg cttctgatga cgattatttt ttcagcagtc ggacaatttg
     1501 gtgatttggt cgaatctgca atcaaacgtc attatgatgt gaaagactcg ggaaatatct
     1561 taccaggcca tggtggaatc ttagaccgtt tcgacagttt gctgtttgta tttcctatca
     1621 tgcatttatt tggcattatt tagtaagtat acctgcgttt ctttgtgaat ttcataaaag
     1681 acgcaggttt ttatttttca ttatattgat actattggtg tctttggtat actatggtaa
     1741 aattatagtg ttttatcgtt gttttataaa aaacacaaaa aacaagtaaa ggagaaaaaa
     1801 gctatgaaaa cgattctgac atttatcatc gtttttggta tattagtgat tgttcatgag
     1861 tttggtcatt tcttctttgc gaaacgatca ggaatcctcg ttcgcgaatt tgctatcggt
     1921 atgggtccga aaatttatgg gcatcaagca aaagacggga caacatatac gcttcgccta
     1981 ttacctattg gcggttatgt tcgaatggca ggcaatgggg atgatgaaac agaaatggca
     2041 ccaggaatgc ctctttctct tttactaaat tctgacggca tcgtagagaa gataaattta
     2101 agtaagaaaa tccaattgac aaatgctatc ccgatggaat tgagccgtta tgatttagaa
     2161 gatgagctga ctatcactgg ttatgtgaat ggagacgaaa cagaagtagt aacctaccct
     2221 gttgatcacg atgcgacgat tattgaaaac gacggaacag aaatcagaat tgcgccaaaa
     2281 gacgtacagt tccaatcggc taaattgtgg cagcgtatgc tgaccaactt tgctggaccc
     2341 atgaataact ttattttggc aatcctgtta ttcatcattc tggcatttat gcaaggtggt
     2401 gttcaagtga cgaacactaa tcgcgtagga gagatcatgc ctaatggagc tgcggcagaa
     2461 gcaggcttaa aagaaaatga tgaggtagtc agtgtggacg gaaaagaaat ccattcatgg
     2521 aatgatctga caacagtcat tacgaaaaat ccggataaaa cattagactt taagatcgaa
     2581 cgagaaggtc aggtgcaatc tgtcgacgtg acaccgaaat ctgtagagtc aaatggcgaa
     2641 aaagttggcc aacttggtat aaaagcccca atgaatacag gttttatgga taagatcatt
     2701 ggagggacgc gtcaagcttt cagcggttca ttggaaattt tcaaagcctt aggatcacta
     2761 tttacaggtt tcagtttaga taaattaggc ggacctgtca tgatgtacca attgtcttca
     2821 gaagcagcaa atcaaggtgt aacaactgtt attagtttga tggcgcttct ttcaatgaat
     2881 ctcggaatcg tcaatctgct tccgattcct gccttagatg gcgggaaatt agtcttgaat
     2941 atttttgaag ggattcgcgg gaaaccatta agtcaagaaa aagaaggaat attgacatta
     3001 gctggctttg gatttttaat gttgttgatg gtgctagtta catggaacga tattcaacga
     3061 ttctttttct agaaaaagtt gacaaatagg gtataataac aaacgataac tattttgagg
     3121 aaataaagga gtatatagaa tgagacaatc aaaaatgttg attccaacat tacgcgaagt
     3181 tccaaatgat gcagaagtat taagccatca aatcttgcta agagctggat atatccgcca
     3241 agtatcagcg ggtatttatt catatctgcc attagcaaat cgtgtattgg aaaaactgaa
     3301 acgcattatg cgggaagaat ttgaaaaaat cggagcggta gaaatgctga tgcctgccat
     3361 cttgccagca gaactttggg aagaatccgg gcgttacgaa acatatggac caaacttgta
     3421 tcgtttcaaa gaccgcaatg atcgaaaaat gatccttgga ccaactcacg aagaaacttt
     3481 tactgatttg atgcgtaatg aaatcaattc atataagaaa ttacctttaa atctttatca
     3541 aatccaacct aaatatcgtg acgaaaaacg acctcgcttc ggtttgcttc gcggaagaga
     3601 gtttatcatg aaagatgcgt attctttcca tgcaacggaa gaaagtttgg acgagacata
     3661 taaagattac gaaaaagcct acaccgaagt attcaagcgt tgtggcttag aatttcgctc
     3721 gattatcgga gacggtggcg caatgggcgg gaaagactca aaagaattca tggcgatctc
     3781 agaaatcggg gaggatacga tctgttattc gactgaaagt gattatgctg caaacttgga
     3841 gatggctaca agctactatg tatcaaaaaa atcgcatgaa acacagcttg aattggagaa
     3901 agtagctaca cctaacgtaa aatcaattga cgaagtagca gatttctttt ctgttgaacc
     3961 tcaaaaaatc atcaagtctg tattgttcat tgcggatgaa caacctgtcc ttgttttggt
     4021 acgcggagac catgaagtca atgacgtaaa actgaaaaac tttttatctg ctgacttttt
     4081 agaagaagca acagaagaag aagcacaaaa atatctaggt gctgagtttg gttctgtcgg
     4141 accagtcggt gtttcagatg acgtaaaagt atatgcagac cgttatgtcc aagatttagc
     4201 gaatgccatt actggagcga acgaaacagg attccactat aaaaatgtga atccagaacg
     4261 cgatttcact gtactttctt atgaagatct tcgttttgta caagaaggag atccttcacc
     4321 agacaacaat ggtgttcttg cctttactcg aggaatcgaa atcggacata tcttcaaaat
     4381 cggcactcgt tacagtgaat ctatgggagc aactgtacta gacgaaaatg gacgagaaaa
     4441 atttgtgatt atgggctgtt atggtatcgg tgtaagtcgt ttattatcag caatcgtaga
     4501 acaacattca gatgaacaag gaatcaactg gccaaaagga attgcgccat ttgatttgca
     4561 tatcgtccaa atgaatctaa aagacgaata ccaaacaaac ctgacgaatg aactagaaga
     4621 agcgatgaca aaagctggct atgaagtact ggttgatgac agaaatgaac gcgcaggagt
     4681 gaaattcgcg gattctgatt tgattggctg tccaatcaga atcactgtcg gtaaaaaagc
     4741 agtggatgga atcgtagaag tgaaaatcaa gaaaactggc gaaatggttg aagtacgtaa
     4801 agaagaattg gtgaatactt taccaatcct cttgaaccaa ggcgaataat aagcgttaaa
     4861 acacagaaaa taaaaatagc tgaatcagct gtttttgaca atctgatgat tcgcttttaa
     4921 aattggaact gcgatttgaa tcgcagttcc aattttttaa tgtaaaactc agaaaaaacg
     4981 agtatactaa aaggtactga aaaagaagag gataagatgc gatcaattgc attgtttctc
     5041 atcagaaagg gcgtatatct ttgtcagaaa aacgagattt atttgaaaaa ctgttggaac
     5101 aaatccaact agaggaagca gaaaaaaatc atccactgct tactgctggt gaaatggata
     5161 gtgtagtggt ccatcgtaaa tcgcgtttat gggaatttac gctgcatttt tccaaaattt
     5221 tgcctattat gctatatcgg tcattgactc agcatctgac tatcgctttt aaagatattg
     5281 cacaagtcaa actgaatatc ttagcagatg accagacatt cgatgaacag ctcttgcaag
     5341 attactgggc ccaagctttg gaaaatcagc aatgcgatac accactagca cagcaagtac
     5401 tgaaaacgca ggtacctgtg ataaaagatc gtaaagtcat cttgccaatc gacagtacag
     5461 gagctatttc ttatttgaag caacaatact tgccattagt agaagagttg ttcgtcagtt
     5521 acggattccc aaaatttcgt atcgaaccag aagtggatga gcagcaagcg gaacgagtat
     5581 tgaaactttt tgaagaacgc aaacaagaac aggcagaagc ctttatgaag caagcagctg
     5641 aaagtctagt tgtccacgaa caaaagaaaa aggaaagaaa agagcaatcg ccagctcttg
     5701 aaggacccat ccacatccag ctaggacgaa atattccagc cgatgaacca acgacaccaa
     5761 tgatcagtat cgttgaagaa gaacgaagag tcacacttga ggggtacgta ttcgacaaag
     5821 aagtcagaga actacgttca aaacgcaaga tcttgacttt gaaaatcaca gattatactt
     5881 catcatttat cgtcaaaaaa ttctcgaacg gcgaaaaaga tgaacaagta tttgatgcga
     5941 tcagtgtcgg tagctggtta aaggtacgag gaagtattca agaagatacg tttgtccgtg
     6001 atttggtcat gaatgcgcaa gatatcatag aagtcaaaca tacacctcgc aaagattatg
     6061 ctcctgaagg ggaaaaacga gtagagcttc atgttcatag caatatgagt acgatggatg
     6121 caacaaacag catctctgat cttgtggcac aagcagggaa atggggacac cgagctatcg
     6181 ccattacaga ccacggtggt gcgcaagcct ttccggaagc acatagtgct ggaaaaaaag
     6241 caggtgtgaa aatcttatac ggcgtggaag cgaatgtcgt agatgatggt gtcccaattg
     6301 cttataatga tgcacatgaa gcattgagtg aagccacata tgtcgtattt gacgtggaaa
     6361 cgacaggatt gtctgcagta tatgacacga tcatcgagct agccgcagtc aaaatgtata
     6421 aaggaaatgt gatcgaaagc tttgatgaat ttatcgatcc aggacatcct ttgtcacgaa
     6481 caacggtaga tctgactggt atcacggatg gaatggttcg aggatcaaag tcggaagaag
     6541 aagtattgcg gatgttcctt gaattttcaa aagatacaat tttggtagcc cacaacgcgg
     6601 cttttgatat ggggttcttg aatactagct acgcaaggta tggtattcct gaagcggcca
     6661 atccagtcat tgatacgttg gaattagcta gatatctgta tcctcaattc aaacgtttcg
     6721 gattaggtgt gttatctaaa aaatttggtg tcagcttgga acagcatcac cgagcaatct
     6781 atgatgctga agcaactggt cacttagcat ggatctttgt gaaagaggca atggacaatc
     6841 ataacatgct ttatcatgat cagctaaatg agcatatcgg agaaggagat tcttacaaaa
     6901 gggctcgacc tttccatgta acgattttag caaaaaatca ggcaggactg aaagatttgt
     6961 tcaaactgat ttcaatgtcg aatgtcgaat acttcgaacg cgtcccacga ataccacgct
     7021 cccaattgaa aaagatgcgt gaaaatctat tgatcggctc agcttgtgat aagggagaaa
     7081 tattcgaagc catgatgcaa aaaggggtag aagaggcaag aaaccgagca aaattctacg
     7141 attatattga agttatgcca aaagcggtct atgcgccttt gatcgagcaa gaattggtaa
     7201 aaaatgaaca tgatttggaa gaaattatcc aaaatttggt agaaatcgga aaaagtttgg
     7261 ataaaatcgt tgttgcaaca ggtaatgtac attatctgaa cgaagaagat gccatttacc
     7321 gaaaaatttt gatcaattct atgggcggag cgaatccgtt gaatcgtcat agtttgccgg
     7381 atgtccattt ccgaacaacc gatgagatgc taactgcatt tcacttttta ggggaagaaa
     7441 cagcgaaaga gatcgtagta gaaaatacga ataaaatcgc agatatctgc gaagaagtga
     7501 ttccagtcaa agatgaactt tatacaccga agattccagg ttcagaagat gaaatatcag
     7561 aattgagtta cacaaaagca aaacaaatgt acggagatcc gttacctgag atcattcaaa
     7621 aacgattaaa aaaagagttg aattctatta atggtaatgg cttctcggtt atttatctga
     7681 tcgcccaaaa actagtgcat aaaagtaatg aagatggcta tctagtaggt tcacgtggct
     7741 ctgttggctc tagctttgtt gcgacgatga caggaatcac tgaagttaat ccgttagccc
     7801 ctcattatta ttgtccagag tgccaatatt ccgagttttt tgaagatggt acgtatggtt
     7861 ctggatttga catgccagaa aaacaatgtc caaaatgtgg tgcacgctta aacaaagacg
     7921 gtcatgatat tccatttgag acatttttag gattccatgg agacaaagtg cccgatattg
     7981 atttgaactt ctcaggtgat tatcaagcag aagcccataa ctatacgaaa gtattgtttg
     8041 gcgaagacta tgtttaccgt gccggaacga ttggtacagt cgccgacaaa acagcatatg
     8101 gctatgtaaa aggatacgaa agggacaata atctgcagtt tcgaagtgca gaagtcgaca
     8161 gattagcaaa aggcgctact ggagtcaaac ggacaaccgg ccagcatcca ggggggatca
     8221 tcgttattcc cgattatatg gatgtctatg attttacgcc gatccaatat ccagcagacg
     8281 atcaaaattc ggaatggaaa acgactcatt ttgattttca ctcgattcac gataatgtat
     8341 tgaaattaga tattctaggg catgatgacc caactgttat tcggatgctt caagatttat
     8401 cagggatcga tccacaaacg atccctacgg atgacccgga agtgatgcgg atatttgccg
     8461 gtcctgaagt acttggagta agtcaggaac aaatttattc caaaacaggg acattaggta
     8521 ttccagaatt cggcactcgt tttgttcgag ggatgctaga agaaacgcat cctacaactt
     8581 ttgcagaact actgcaaatt tccggactat ctcatggtac agacgtatgg ttaggaaatg
     8641 cggaagaatt gattcgacga ggagatgcga cactggcgga agtaatcggc tgtcgtgatg
     8701 atattatggt ttatttgatc catgctggat tagatagcgg gatggcattt aaaatcatgg
     8761 aaactgttcg taaaggacaa tggaacaaga tccctgatga attgcgagaa acctatctat
     8821 cggcgatgaa agaaaataat gtgcccgact ggtatatcga ttcttgttca aaaatcaaat
     8881 atatgtttcc gaaagcccat gctgcagctt acgtattgat ggctcttcgt gtcgcttatt
     8941 tcaaggttta tttccctatt ttgtattact gtgcatactt ttctgtacgt gcagacgact
     9001 ttgatctagt ctccatgtgc aaaggaaaag atgctgtgaa acaagcaatg aaagaaatca
     9061 cggacaaagg attagatgct tccgtaaaag aaaagaatca actgacagtg ctggagttag
     9121 ccaatgaaat gctggaacga ggattcaaat ttggaatgat tgatttgtat aaatcggatg
     9181 ccgttaactt tgttattgaa ggtgatacgt tgatcgcacc atttagagct gtcccaagtt
     9241 tgggaaccaa cgtggcaaaa caaatcgttg aagcaagaaa agacggtcca tttttatcca
     9301 aagaagatct tgccacccgc ggtaaagttt caaaaacatt gatcgaatat atgaacgaca
     9361 atggcgtatt gaaagatcta ccagatgaaa atcaattatc gttattcgat atgctataag
     9421 catctgtttt cctgtctgct tgtaatttta agcagagtat ggtatagttc ttataggtaa
     9481 tgatactaac tgtgagtgag cggatttttc gctcactctt tttaatggaa ttacgcactt
     9541 aattttttaa ggaggcgaaa cttttgagta gcgtcgttga aacagtcaca gaaatggtga
     9601 caccgatttt agatgaacaa aattttgaac ttgtcgaagt cgaatttgta aaagaaggaa
     9661 aaagctggtt cttacgagta tttattgaca aagaaggcgg tatcgatatc gaagaatgcg
     9721 cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc agatccagat ccgattcctc
     9781 aagcttattt cctggaggta tcctccccgg gagcagagcg gccattgaaa aaagaaagcg
     9841 actatgaaaa ggcacttggt gattatgtcc atgtctcctt ataccaacca gtagatggcg
     9901 aaaaacaata tgaaggattt cttcaatcat ttgatgctga acagcttaca ttgaaaatac
     9961 gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa aaatattgca aaagctcgct
    10021 tagctatcca attttaatca cggagaggaa tcagaaaaaa tgagtaaaga aatgttaaat
    10081 gcgttagacg ctttagaagc tgaaaaaggg atctcaaaag aaatcgtgat cgatgcttta
    10141 gaagcagcat tggtttctgc atacaaacgt cactatggcc aagcacaaaa cgtcgaagtg
    10201 gaatttgatc agaaaaaagg aaagatccat gtctatgcgg taaaagaagt aacggaagaa
    10261 gtgatggatt cacagttgga agtctcattg aaagatgcac ttttaatcaa tccagcatat
    10321 gagatcggtg atacgatccg ttttgaagta acaccaaaag attttgggcg gatcgctgcg
    10381 caaacggcta aacaagttat tttacagcgt gtacgtgaag cagaaagaac gattatttat
    10441 aacgaattca gtgcttatga aaaagacatc atgcaaggaa tcgtcgaacg ccaggataaa
    10501 cgttatattt atgtcaatct tggtaaaatt gaggcagttc tttctaaaca agatcaaatg
    10561 ccaaatgaat tttatcaacc acatgatcga atcaaagttt atgtttcccg agtagaaaat
    10621 acgtctaaag gtccgcaagt atttgttagc cgcagccatc cagacctatt gcgccgctta
    10681 tttgagcaag aagtaccaga agtctatgat ggaatcgttg aaattgtcag catcgcccgt
    10741 gaagctggcg atcgttcaaa agtggcagtt cgctcaaatg atccaaatat cgatgcagtc
    10801 ggtacatgtg tcgggccaaa gggacaacga gttcaagcaa tcgtcaatga attaaaaggc
    10861 gaaaacatgg atatcgtcga atggaatgaa gatccagctg tattcattgc aaatgcattg
    10921 aatcctgcac aagtcatgga tgtgatcttc gatgagacaa atccaaaggc ctgcacagtt
    10981 gtcgttccag actaccaatt atcgctagca atcggtaaac gcggacaaaa cgcacgttta
    11041 gctgctaaat tgactaacca taaaatcgat atcaaatcag aatctgacat gacggaattt
    11101 tatgaaaatc aagaacaaca aaaagaaaca gaagaactgc atgatgaagc aatcgttcag
    11161 tcagatttaa cagacgatga atatgaaaca attgcattca acaatgaaac ggtcgaagaa
    11221 aaaccagaag cttaacttta gtaaggaggc aaagagatga aacaaaggaa aattccttta
    11281 cgcaaatccg tggtttcagg tgaaatgaaa cctaaaaaag agatggtccg aatcacccgt
    11341 tcaaaagaag gggaagtatc gatcgatcca actggaaaaa tgcctggacg aggagcgtat
    11401 gtctcacttg agccggaaga agtacaacaa gcttgggata aaaaaattct cgatcgagta
    11461 ctcgaaacaa cattaacaga tgagttttat caagaacttc ttgactatgt cactcaccaa
    11521 aaagcacgta aagagttatt tggaaaatga atacagttaa tcggcaaaaa gccatgaatc
    11581 tcatcggtct agcgatgcgc gcgggaaaac taatcacggg tgaagaactg accattgctg
    11641 aaatccgcaa acaaaaagca aaattggtgt ttgtggcaac cgatgcaagc gagaatacaa
    11701 gaaaaaagat aaaagataag agttcatatt atgaagttcc ttgctttgag ctattttctg
    11761 aagcagaaat cacgcagatg atcggaaaac ctcgcaaggt attcggaata atggatgccg
    11821 gatttgcgaa gaagaccaag gagctaattg aaggttagga aggtgattgc atgggcaaca
    11881 aaagaatcta tgaattagca aaagaactca aaaaatctag taaagacgtc gtggaaaaag
    11941 cacaacaact tggcatcgat gtgaaaaacc atatgggtgc gattactagc aatgatgaga
    12001 agaaattgca acaagcgttc aaaaacccaa gcacgaaaca acaagctgca caacaagcaa
    12061 gcagaaaacc agccaatcaa cagccgaacg atcaacgtaa aaaagaaaca acgaacaatc
    12121 aacaaaaaaa taaaaataac cgcagttatc aagatcgcgg tcaagggagc ggtcaagtga
    12181 atcaaggaaa aaaaccaaca acaaaccaaa ataatacggg aaataccagc agccaaaatc
    12241 gtcaaggaac gagccaaggc ggcaatcaaa accgtactaa caataacaac aaaccaaaca
    12301 ataacaatcg cggcaaattt aataataata accgtaatcg ttataataaa aaaggtaaaa
    12361 aaggcaaaca gcaacaatca actaaaccag ctgtaccacc acgcaaattc cgtgaattgc
    12421 cagatgtatt agagtatact gaaggtatga acgtagcaga tattgcgaaa aaaattcatc
    12481 gtgagccagc agaaatcatt aaaaaattat ttatgcttgg tgtcatggtc aatcaaaacc
    12541 aagcactgga taaagatacg attgaactgt tagcgacgga ctatggcatg gaaccacaag
    12601 aaaaagtcca agttgacatt gctgatatcg ataaattctt cgaggcagaa gaagtcgatc
    12661 cagataaatt agtctcacgt ccgccagtcg taacgattat gggacacgta gaccatggga
    12721 aaacaacttt attggataca ttacgccatt cacgtgtaac aagcggcgaa gccggcggaa
    12781 tcacacagca tatcggtgcc taccagatcg atattgatgg taaaccaatc actttcttgg
    12841 atacaccagg acatgcggcc tttacaagta tgcgtgcacg tggagcaagt atcacagata
    12901 tcacgatctt agttgttgct gcagatgatg gcgtcatgcc acaaacagtt gaagcaatca
    12961 accatgccaa agctgcaggc gttccaatca tcgtcgctgt aaataaaatt gataaaccag
    13021 gtgcaaaccc tcaacatgtt atgcaagaat taagtgagta tgagttaatt cctgaagcat
    13081 ggggtggaga aacgatcttt gttgagatct cagcgaaatt cggccaaaac atcgaagaat
    13141 tgctggaaat gatcctgtta gtcgcagaag tagaagattt aaaagcagat ccaacacaac
    13201 gcgcaatcgg aacagtgatc gaagcacgtc ttgataaagg taaaggtcct gtgtcaacct
    13261 tacttgtcca agaaggaaca cttcatgttg gtgacccaat cgttgtcggt aatacttacg
    13321 gacgtgtccg tgtaatgaca aacgatcttg gccgtcgcga taaagaagca ggacctgcaa
    13381 cgccagtaga aatcactggc ttgaatgatg tacctcaagc aggtgaccgt ttcgtagtct
    13441 tcgaagacga aaagacagca cgtgcagcgg gtgaagaacg cgcgaaacgt gcattattag
    13501 aaaaccgtgc tgccacaagc cgtgtgactt tggataactt atttgaaagc ctgaaagaag
    13561 gcgaattgaa agaagttaac gtaatcatca aagcagatgt acaaggttca gcagaagcac
    13621 tggctgcatc attgaagaag attgatgtgg aaggtgtccg tgtaaaaatc gtccattcag
    13681 ctgtcggagc aatcaatgaa agtgatgtaa ccttagcagc agccagcaac gctattatca
    13741 tcggtttcaa cgttcgtcct acaccgcagg caaaagtgca agctgatact gaagaagtcg
    13801 atattcgtct tcaccgtatc atctataaag ccatcgaaga aatcgaaact gcgatgaaag
    13861 ggatgctaga ccctgaattt gaagaaaaaa tcaccggtca aatgatcgtt cgtgaaacct
    13921 tcaaagtttc taaagttggt acgattgcag gtgcctatgt aacagaagga tatattcgta
    13981 gagatagcgg tgtccgtgta atccgtgacg gaatcgtaat ctatgaaggc caacttgcaa
    14041 gtctgaaacg attcaaagat gacgtgaaag aagtcaaaat gggttatgaa tgtggtgcaa
    14101 tgattgaaaa attcaatgac atcaaagtag atgacgtaat tgaaggattt atcatggaag
    14161 aagtcaaaaa tgactaaaaa ctgataaaag gaggccaatc attatggcta actaccgtga
    14221 ccgccgagtc ggtcaagaaa tcttaaaaga agtcaatgat attttgcgca aaaaggtccg
    14281 tgaccctcgt gtggagaacg taacgatcac agatgtgaaa gttacggggg acttacagca
    14341 agcaacgatc tattacagca ttttatctga cttagcatca gataagaaaa cagcacaaga
    14401 tggattgaat aaagcatcag gattgattcg tcgagagtta gggcacaatc taagtattta
    14461 taagacacct gaattgttct ttgaacttga tgaatcagtt gtctatggca accatatcga
    14521 tgaattgctt cgcgacttga acaaagacta atcaaaaact cccgcaaatc ctgtgaaatc
    14581 aggggttgcg ggattttttt gttttcatta agacgtatta cctaagagtt ccatgacttt
    14641 agtcttgcgg cgtgtcagct aaccaaaaaa gagaaaaaga aaagtaaaaa attcgacttt
    14701 cttttttcta gtagtatata aatttttgca tatagataaa gatcaagata ccgataacgg
    14761 aagctagatt gtaatctatt ttagtgacag tcaggtatag ccaagtaact agaagatcag
    14821 tggaacctgc accataaaag tttgagtttg tcggtccgtt gacagttaaa agaaaaatca
    14881 cgttgaagtt attgatattt ccgattacgc cgcttcgatt tggtcacgat ccaaattttg
    14941 gataatccct gtagaaatta gcatagtagc tgggatacca attcacatat tgacaaagat
    15001 aacagagact tttacataaa acggatcaat caggaaagga attgctcgat ccagccccag
    15061 ttttccagca atgtgttaac tggcccagcg tcattcagca aattatgcat gatgaggagc
    15121 gaaatgaact gcggaacagc cattgtgata acgacaagtt ttttggatca atgtaaaaaa
    15181 cgtcagcgaa aggaaaaatt tcgtggtttt ttatacctgt tcatttggct agatataaaa
    15241 tccaaacaaa tgaacagaag tgcttattag gtttcaattg ggagtatctg cgctagaata
    15301 agagtaataa tgaaaaggag tgttatgtat ggattattct agtaaagaat actttgataa
    15361 aatgacggct tggtggcgtg ctgctaatta tctttctgtt gggcaacttt atcttaaaga
    15421 taacccctta ctgagacgta cactgaaacc cgaagatgtc aaaaaacatc caatcggtca
    15481 ttggggaacg attcctggcc aaaattttat ttatgtccat ttgaaccgag tcatcaataa
    15541 atatgatttg aacatgtttt atattgaagg gccaggacac ggcggacaag tcatggtatc
    15601 gaatgcttat ttagatggaa gttacacaga gatctatcca gaagtaacgg aagatgagac
    15661 aggaatgcaa aaattattca aacgattttc atttcctgga ggcattgctt ctcatgcagc
    15721 accagaaaca ccaggttcta tccatgaagg cggagagctt ggatactctc tttcacatgc
    15781 agtcggagct gtattagaca atcctgaagt aatctctgct gtcgtgattg gtgatggtga
    15841 agcagaaacc ggtcctttag ctggaagctg gttctcaaat gtctttatca atccagtgat
    15901 agatggagct gttttaccga tccttcattt aaatggtgca aaaatagcca acccaacgat
    15961 cttggcacgt aaatcagatg gagaattagc aaattacttt aacggacttg gctgggaacc
    16021 tttctttatt gaaggaaatg attcagaaaa attaaatcct gttatggctg aaaaaatgga
    16081 tcaagcaatc gagaaaatta aatccatcca aaaagaagct cgcctgaaga cagcaacaga
    16141 tgtcgtgatg ccaaaatggc cagtattgat cgtacgcaca ccaaaaggat ggaccggtcc
    16201 aaaagaatgg gacggggaac caattgaagg aaccttccga gcacaccaag taccgattcc
    16261 tgtagatcaa gaacatatgg atcatgcaga tgctttactc cgttggctga aatcctatga
    16321 accagaaaaa ttatttgatg cacaaggacg tattttagaa gaaatcagag aaatcgcgcc
    16381 tactggtgac caaagaatgg caaaaaatcc tatcaccaat ggtggaatcg atccaagacc
    16441 gcttattatg ccagattgga aaaaatatac tttgcaattt gaaaaaccag gctccgttaa
    16501 agcagaagat atgaccgaac ttggtaaatt cgttcgcgag atcattgaga aaaatccaga
    16561 aaatttccgt atatttggac cagatgaaac aaaatcaaac cgattgaatc aagttttcaa
    16621 aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa aatgatgaat ggctttcgcc
    16681 atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa gatgaagggt tcttagaagg
    16741 gtacgttctg actggacggc atggtttttt gcaagttacg aatcattctt gcgagtggtt
    16801 gactctatgt tgactcagca cttcaaatgg atgcggaaat cacatgacct ttcttggcga
    16861 aatgattatc cttcgctaaa tctcattgca agctcaacag tcttccagca agatcacaat
    16921 ggatatagcc accaagatcc aggaatattg acacatttgg cagaaaagaa ggcggagttc
    16981 atccgtgaat atctgcctgc ggatgcaaat acattgctcg cagtcatgga taaagcattc
    17041 cgctcgtcag aaaaaatcaa tttgatcatc tcttccaaac atccgcgtgc tcaattttac
    17101 tcagcagaag aagcagccgt cttagtgaat gaaggcttga aaatcattga ttgggcttcg
    17161 acagcaaaag aggaggaacc tgaattggtc attgctgctg caggaacgga atctaactta
    17221 gaagcattag cagctgtaac tttgctgctg gaagaattcc caaaactaaa aattcggttc
    17281 atcaatgtcg tagatttatt gaaattacgt catccaagtc aagatccgcg tgggttgagc
    17341 gatgaagaat ttgatcaata ttttacaaaa gacaaaccga tcttatttgc tttccacggt
    17401 tatgaaacat tggtccgcac gattttcttt gatcgtcata accatcattt aatgattcat
    17461 ggctacaaag aaaatggaga tatcacgaca ccatttgaca tgcgtgtagt aaatgaactt
    17521 gatcgttatc atttggcaaa agacgcagct ttaaaaatca aaggaagtca agcagaagat
    17581 tttgcgaaaa aaatggatca gaaattacaa gaacatcaaa actatattcg tgaaaatggt
    17641 attgatctgc cagaagtatt ggattggaaa tggaaaaatc ttgaccaata aaatgataaa
    17701 atcgatcccg caaaatagaa ttttttacat tgattaagaa actgaaaaaa taaataaaaa
    17761 aaacggaaaa aagactggaa gaatggccag tctttttttt cgtttttggg aagtgaaaac
    17821 caaactattt acctgtaagc cacccataaa tccaatcaaa tggtgcaaag aaaaaatctt
    17881 ccattctatt ccctccttta aaaaagcata acatgatttc tatttacgtg agcatgaatt
    17941 ttgttacgtt tttattgccg aaaaataaaa accgttcatt atgatgtcaa attcgtcagt
    18001 aattgatgca atgtgttata ataattcagt taaatcaaaa atgaaatgga gaaaagacat
    18061 ggacgggtta ttacctttat ggaaagaacg cggaatgaca agtcatgatt gcgtattcaa
    18121 attaagaaaa atcttacata cgaaaaaaat cggacatggg ggtacgcttg acccagacgt
    18181 agacggtgtg ttgcccattt gtattggaaa agccacaaaa gttatcgaat atttgacaga
    18241 ttctgggaaa atctatgtag gcgagataac tctaggtttc agtacaacga cggaagatcg
    18301 atccggagaa cgggtagaaa caaaaccagt agatatgtct ttatctgaaa aaaaaataga
    18361 tgaagtgatg gcttcattta ttggtgagat cacccagata ccaccaatgt actcagcggt
    18421 aaaagtcaac ggtaagcggt tatatgaata tgcccgcaat aatgaaacag tggagcgtcc
    18481 tgttagaaag gctcagattt accgttttat ccggacaagt gaattgacaa gagacgaagt
    18541 gacaggaacc atttcttggc gcttcgaagt agaatgcggg aaaggaacct atgtccgcac
    18601 attagctgta gatacaggca agaaacttgg ttaccctgcg cacatgtctg atctgacccg
    18661 aacagcaagt gccggtatgt cccagaagga tgctctgaca ttagaagaag tagctcaagc
    18721 tgtgaaaaat gggacaatcg atgattgttt gctgccaata gaaaaaggag tggaacggtt
    18781 caaacgagta gatattactg atgaagtatg gcagaaagta aaaaacggta tgagacttga
    18841 ttatactgta tttgcgctcc cagaaatgcc gtcagaagaa attgctttgt tttataaagg
    18901 aaaagttgtc agtatttatc agccaaaccc gaaagaaaaa aacaaattaa aacctagtaa
    18961 agtattgaga aatgaggaat aacgaatgaa tatcatcaag atcagacatc cttaccaatc
    19021 agaacaaatt ccagaagacg atgtcgtttt agtgctggga tttttcgacg gcgtgcatat
    19081 cggacaccag aaagtcataa agacaggtat ggaaattgca caaaaagaag gattgaaatt
    19141 agcattgatg actttcaatc agcatccatc tattgtcttt aaaaagatcg atcctagcag
    19201 cgtgaaatat ttgactagcc ttaagcaaaa agaagaaaaa atggccgctt tgggtataga
    19261 ttatttatat gaagttgatt ttacttcttc ttttgctcat ttagcaccgc aggaatttgt
    19321 tgatcaatac atcgtagggt tacatgcaaa atatgctgtt tccggttttg attatacgta
    19381 tggaccaaaa gacattgcag atgtcgctca ttttcctgaa tatgccaaag gaagattcaa
    19441 aatcgtcaca gtttctaaag aagaagaaca aggtgaaaaa atcagctcta cccgtattcg
    19501 tggattacta gacagcggac aagtaaaaga agcagcaaaa ctattaggtt gcacatatga
    19561 ggtagaaggg gtagttgtcc atggcgatgc acgaggcaga ctactaggat tccctacagc
    19621 aaatgtcaaa gtaaagagca cggttcattt gcctaaagaa ggtgtttatg tgtcggaaat
    19681 aaaaattggg gaaacatggt atccagccat gggttcaatt ggacataatg atacattcgg
    19741 tgaaggacgg gagctgactg tagagttgta tattttggac tttaatcaag atatctatgg
    19801 tgaacatgtc gcggtacgct ggaatcaatt tttaagagat caagtgaaat tttctagtgt
    19861 agaggaattg attgaacaac tcaaggagga tgaacaagca acagctgaat atttttctca
    19921 gcaataaaac attatgcata aaatctcttg tttatgtata actagcaatt acaataaaat
    19981 gggtgataaa taagcccagg aaaggggctg tgacaaaagt cttgtcacag cctttttttc
    20041 caaataaatg gtttttcaga agtagcttct tcggaaataa gccgaaattt cttgaagctg
    20101 accacttctg tcacaacctc tttctttata gaaataaaag gacactagca attcatgaaa
    20161 acgattgcta gagcgtatca tttgctttag actatatatc ttggtgtaca ctcactttaa
    20221 ggcggaaata aaagttatcc gtaataacag gtttaggtat gaaaaaggag tgggtactat
    20281 gtacaaggaa ttagaaaaca aagtcgcagt agtaactggt ggctcaaagg ggattggaac
    20341 agcgattgct caacgattcg ggaaagaaaa aatgaatgta gttgtcaact ataactcgga
    20401 taaagaagga gcagaaaaag cagtcaaagc aatcgaagag gcaggcggaa aagcagtctc
    20461 tgtacaagca gatgtcagtt ctgaagaagg aatacagcgt ttacttgata cggctttaga
    20521 aaattttggg acactggacg tttgggttaa taatgctggg atggaaaatc agcatccaac
    20581 ccatgaactt agccttgaag attgggaaag agtgttaaaa gtcaatttga ctggcgtttt
    20641 tatcggaacg aaagcggctt taaattactt tacagagaat aataaaaaag gaaatatcat
    20701 caatatgtct tctgttcatg aacagatccc atggccaacg tttgctcatt acgctgcttc
    20761 aaaaggcggg gtaaaattat ttaccgaaac ggtagcaatg gagtatgcac cgaagggaat
    20821 cagaatcaat agtataggcc ctggtgctat ccgtacacct attaatgcgg caaaatttga
    20881 tgatcctgaa caaaaagaac tcttggaaag tatgatccca ctaggacgcg taggtgaacc
    20941 aaaagaaata gcggcgacag ctgcatggtt ggcttcagac gaatccagtt atgttacagg
    21001 tattacacta tttgcagatg gtgggatgac tttatatcct tctttccaag gtggaaaagg
    21061 ataagggtga ataaaaatgg gtttattgat tgcattgatc ccggcagttg gctgggggat
    21121 ccagccactg attttgaaaa agattggtgg cagaccaaca aatgagatat taggaactgg
    21181 tattggagcg gtgcttgtcg gcttactcgt tcaattattc gcttctccag ctggtatttc
    21241 agtgactaca tttttgatta gtttactttc tggtgcattt tgggttattg gacaaacagg
    21301 acagtataca tctttcaact tgattggtgt ttctaaaacg atgccaattt cgactgcatt
    21361 acagttagtc ggaacctcgt tgattggtgt cttcgctttt ggagaatggt ctggaagtat
    21421 ggggaaaatc attggagcag cagcaatcat tcttcttgta tttggttctg ctttaacggc
    21481 aatcagtgat gatcctggaa aaaaaggcgg tatcacaaaa ggaattagtc tattagcatc
    21541 aacaagtatt ggttattggg tatacagtgc attgcctaaa ctagttgatg caagcggact
    21601 ttccattttc tttccgcaaa tgatcggtgt ctttttaggg gcagtgattt atgtgatttt
    21661 ccgtaaacca accgcatttt ctgaagacaa gagttggaaa gcaacgatcg ttggagccat
    21721 ctttagtgtg tcagcattgg cttatatttt ttcagcaaat gaaaatgggg ttgctactgc
    21781 atatatcatt acacagctaa acgtagttat ctctacatta ggcggtatgt acatcctgca
    21841 tgaaaataag acaccaaaag aaaaaaaatt aacgattatc ggattattgc tgatcgtagt
    21901 aggaagcatg gtcacggtct ttatttgagg ataaagatgg taaaatttca tttattaaaa
    21961 aaatatttgt ttgaaccaaa cagtaagtac ttataaaaaa atagcgctag aatttccaac
    22021 atctctggaa tttctagcgc tttttaattg tttaagatac aagggaaata tctgtctgga
    22081 tcaataattt ttcattgatc cattgactaa agtttacgac ataattgaag ttaccaccga
    22141 ttagtatctc cgcatgattg tctttataaa gatcgtattg taaaagacgg tcttttttat
    22201 ttgcgtaaac atcgttatgg tactggacta attgttctcg gaattgattg attgctgtaa
    22261 aatcacgata ttgaaggttg aaacgcatcg tgattggtgc atttagcatg aatcgctgca
    22321 tttcttttgc tgcttcctct gcttgatctc cgtataggaa ctcaaaaaga ttacgttcag
    22381 aaaatttcca ttgcccgcca acctttgttc cttttagttt tccttcatga agataatttc
    22441 tgatagaacg tgtagtaaca cctaattgtt cggctaattc atttactgag taaaaatctt
    22501 tcaatgttca ccctcctatg tgaatggttc tactgactta gttgatattt gcttaatcac
    22561 gaaatgtaga aagaacgatt ttaattaagg tcattgtaaa aatcaagtca gaaaattcta
    22621 acttttattt ctctaaagaa attcg
//
LOCUS       DAZUMT010000044        22576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000044 DAZUMT010000000
VERSION     DAZUMT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.044"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(250..702)
                     /locus_tag="ACG8FM_001490"
     CDS             complement(250..702)
                     /locus_tag="ACG8FM_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5094157.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(715..1125)
                     /locus_tag="ACG8FM_001491"
     CDS             complement(715..1125)
                     /locus_tag="ACG8FM_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5094158.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1400..1612
                     /locus_tag="ACG8FM_001492"
     CDS             1400..1612
                     /locus_tag="ACG8FM_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFS5094159.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1615..1827
                     /locus_tag="ACG8FM_001493"
     CDS             1615..1827
                     /locus_tag="ACG8FM_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094160.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1967..2122
                     /locus_tag="ACG8FM_001494"
     CDS             1967..2122
                     /locus_tag="ACG8FM_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094161.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2119..2343)
                     /locus_tag="ACG8FM_001495"
     CDS             complement(2119..2343)
                     /locus_tag="ACG8FM_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS5094162.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2421..2609
                     /locus_tag="ACG8FM_001496"
     CDS             2421..2609
                     /locus_tag="ACG8FM_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094163.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2748..2876
                     /locus_tag="ACG8FM_001497"
     CDS             2748..2876
                     /locus_tag="ACG8FM_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094164.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2935..3270
                     /locus_tag="ACG8FM_001498"
     CDS             2935..3270
                     /locus_tag="ACG8FM_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094165.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3503..4444
                     /locus_tag="ACG8FM_001499"
     CDS             3503..4444
                     /locus_tag="ACG8FM_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFS5094166.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4446..5336
                     /locus_tag="ACG8FM_001500"
     CDS             4446..5336
                     /locus_tag="ACG8FM_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFS5094167.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5379..6179
                     /locus_tag="ACG8FM_001501"
     CDS             5379..6179
                     /locus_tag="ACG8FM_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS5094168.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6194..7045
                     /locus_tag="ACG8FM_001502"
     CDS             6194..7045
                     /locus_tag="ACG8FM_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5094169.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7084..7245
                     /locus_tag="ACG8FM_001503"
     CDS             7084..7245
                     /locus_tag="ACG8FM_001503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094170.1"
                     /translation="MLQIIGDRDMDELSQQESRMVRKISKLIIYRDTERFLTVLGNVY
                     ENPELLEVE"
     gene            7246..7707
                     /locus_tag="ACG8FM_001504"
     CDS             7246..7707
                     /locus_tag="ACG8FM_001504"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5094171.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7733..8185
                     /locus_tag="ACG8FM_001505"
     CDS             7733..8185
                     /locus_tag="ACG8FM_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094172.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8291..8485
                     /locus_tag="ACG8FM_001506"
     CDS             8291..8485
                     /locus_tag="ACG8FM_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094173.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8516..8809
                     /locus_tag="ACG8FM_001507"
     CDS             8516..8809
                     /locus_tag="ACG8FM_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094174.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8839..9279
                     /locus_tag="ACG8FM_001508"
     CDS             8839..9279
                     /locus_tag="ACG8FM_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5094175.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            9354..9821
                     /locus_tag="ACG8FM_001509"
     CDS             9354..9821
                     /locus_tag="ACG8FM_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFS5094176.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10568..10798
                     /locus_tag="ACG8FM_001510"
     CDS             10568..10798
                     /locus_tag="ACG8FM_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094177.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10957..11790
                     /gene="terS"
                     /locus_tag="ACG8FM_001511"
     CDS             10957..11790
                     /gene="terS"
                     /locus_tag="ACG8FM_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFS5094178.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11783..13198
                     /locus_tag="ACG8FM_001512"
     CDS             11783..13198
                     /locus_tag="ACG8FM_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5094179.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13210..14739
                     /locus_tag="ACG8FM_001513"
     CDS             13210..14739
                     /locus_tag="ACG8FM_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5094180.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14657..15709
                     /locus_tag="ACG8FM_001514"
     CDS             14657..15709
                     /locus_tag="ACG8FM_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFS5094181.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15711..16028
                     /locus_tag="ACG8FM_001515"
     CDS             15711..16028
                     /locus_tag="ACG8FM_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094182.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16155..16778
                     /locus_tag="ACG8FM_001516"
     CDS             16155..16778
                     /locus_tag="ACG8FM_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFS5094183.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16793..17683
                     /locus_tag="ACG8FM_001517"
     CDS             16793..17683
                     /locus_tag="ACG8FM_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFS5094184.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17706..17921
                     /locus_tag="ACG8FM_001518"
     CDS             17706..17921
                     /locus_tag="ACG8FM_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094185.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17933..18274
                     /locus_tag="ACG8FM_001519"
     CDS             17933..18274
                     /locus_tag="ACG8FM_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094186.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18274..18645
                     /locus_tag="ACG8FM_001520"
     CDS             18274..18645
                     /locus_tag="ACG8FM_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094187.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18638..19033
                     /locus_tag="ACG8FM_001521"
     CDS             18638..19033
                     /locus_tag="ACG8FM_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFS5094188.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19035..19412
                     /locus_tag="ACG8FM_001522"
     CDS             19035..19412
                     /locus_tag="ACG8FM_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFS5094189.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19413..20021
                     /locus_tag="ACG8FM_001523"
     CDS             19413..20021
                     /locus_tag="ACG8FM_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS5094190.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20021..20362
                     /locus_tag="ACG8FM_001524"
     CDS             20021..20362
                     /locus_tag="ACG8FM_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094191.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20604..>22576
                     /locus_tag="ACG8FM_001525"
     CDS             20604..>22576
                     /locus_tag="ACG8FM_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS5094192.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINL"
BASE COUNT         8095 a         3314 c         4623 g         6544 t
ORIGIN      
        1 ttcttcctta cttaatataa atctttctct atcatgtaat ttatttagtt tttcatcatt
       61 tttttgttct aattcataag cttctttttt tattgtgtta gctttcgaca gtttagtgat
      121 tcctataatt aataaaataa gtccaatcaa taataacatt caaaactcct catttctttg
      181 atataattat tgtagagttt actcagaaat gaggaaagag tccatgtgaa agcatgggct
      241 tttttatttt taatgttgta atactaaatg ttcaagatta tgaggaagcc ctaaatattc
      301 aataatttgg aactgactca tatcagataa atcttgttca tctattgttc tagttaacat
      361 ttcaattgca aacatattag cttcagcttc tataccataa atcatacaac taaggttgtt
      421 tgctctaaaa gctgctgtgc aaataccttt atggagtcta acgtgggcta tttcatgcca
      481 taatacaaat cttttcatag aaggtgatag gttttgatta attaatatca cataacatct
      541 tttattgtgt atcgtttgtc ctaatgtttt attccctaaa tcagtttcaa ttaaatctat
      601 atcagaatat ttgattattt tatatggatt aatcgcatca gatccaaact ctacaaataa
      661 tttttctatt tcttctaata tccagtattt gtttaaatgc ataccaaagc ccacctattg
      721 acgatatttt ttaggagtga attttttctt tgctttaact ttggttaagg caagtgcaag
      781 ttgaatggca tcgtataatt tttccttatc ttcatcgtct aaaggttcac cataataatt
      841 tgtttcagca tctgaattca agccttcaag taatttttca gcttgtaaag caatatcttg
      901 tttatctttt tctgttaaat cataataatg gcgtttatca gtacgtccta aaagataatc
      961 agttgataca ttaaagtaat ctgcaacttt ttgtatttta tctccagatg gagtactttt
     1021 attccatcta gacatgcttc catttccgaa atttaacttt ctttcaagtt ctgccaacgt
     1081 cattgaacgt tcttttaata agatttttat acggtctaat agcataggat atgcattcct
     1141 ttcaaaatac cgacaaaaaa agtatttttc tgcaaattat ctatttacaa atagagaatt
     1201 tgcagttata ctagttttgt aagcaagata tttacggcat acaaagacta aaagcaataa
     1261 actaatagtc tccccagaca tcagtaagct cgctttttgt gcgctgtatt ttgtatgctt
     1321 acataatagc atatttgcag tttttgtcaa acttattaga caaaaatatt agaaaatatt
     1381 cgataagggg gtatgaagca tgactttaaa acagcaagta ttaattgctt tagtaaagaa
     1441 aggttggagt caaagagagt tagctcgacg aatgggtata tcaattactt atttacgaga
     1501 tatcttcatc gaaaaaagaa aacctaagga acgtttaaaa caaatcgaag aacttttgga
     1561 tatcaagtta gaagtagatt ctaatgatca gccatcagaa caggaggcgt aaaaatggta
     1621 caacgtttga gcaaagcgga gtttaaagag aaatataagt attctgaaag tacttatcaa
     1681 agaagaatga aggaatttaa aaaatcacgt tttagtgagg ggtataacgc agttacttcc
     1741 aaagaaataa caattgatgt cgatttatat gaacagttta gaacatggcg ttctaaaaat
     1801 cgatttagag ttagagctgt ttgttaatag ctaaaaaatc taaattcacc acttctgaaa
     1861 agaggagttg ctgctctcct ttcaagtttg ccaaagtcca cgttttttca ttttggcaac
     1921 tcctcttctt ggaggtggtg acggcgagag acgaaggagg gatgacatga aggaagaatc
     1981 aaaaataggg attttgaatg agaacaatta cacaagatta attattgaaa cagaagaagg
     2041 gaaaaaagta gcagaagtta cattgaccga tgcaactcct gctgacggtt atcgtattcg
     2101 attaactcct aaatatgatt aaccttttgg tgggtgtgga tcgtttccgt gactatcacg
     2161 gctttgaatt tttccatctc tgccatgaat aaatagctca gatccttgat ttttagaaat
     2221 ctctctagcg atttttactg cttctgattt tgtattagta tgtgcagtcg ctttagaatt
     2281 tccagcacct ttgacattcc aaccaccatt ggaattaggt acaacatgtt gattttttcc
     2341 catagttttc acctccattt ctatagaaat attttatcaa atagaaatgg agaaattcaa
     2401 taaacgaaag gagaagtgat atggcatata ccttgcaaca agaacatcaa attctcggtt
     2461 tgattaaaca acgcaggaaa caattacaag atgaccgtgc agcgcttaga aaagccgatg
     2521 agctatcaga taggcaagct gaactaattg cttctgaact tgaggatttg agaatgctag
     2581 aaataaaaaa tagggagatt agattatgaa gaagacagac acacttttta taggattcat
     2641 tttggggtta ttagtgattg tagcgcacca aagtattatc gggggaagct tgttcgcagc
     2701 attgatggtt ttaatcaatc tgcttgattc aaaagaaagg agcaactatg gcacgagaag
     2761 aagcgctaaa aatcggtaaa gtgattgctg ataatcggtg gggaaatagc cgccctatta
     2821 ttttaagcaa gcaacatatc gaaaagcaaa aagcatggca acaaataaaa aagtgactct
     2881 gccagccagc aaagagtcac atacaaattt ataaaactag gagaattata tcacatgaat
     2941 aagaaaattg aaaatcttat cgaagaattg aaacgtgaat gccaaaagca aggtgtttct
     3001 attatttgta cagcccaaaa agaaggggaa cttaaaagtc tcgtttatgg tgagacaaca
     3061 gaaattttac tttgtcttgc tatgcaagaa gaacatttag atgaaaatct cccattgtca
     3121 gcacatatta tgcgtagaat tgctgtggat gcttacgaac aagcgaaaaa tgaagaagaa
     3181 aatcaacctt caaatcatac ttttgttatt aataacaagg aagatttagc tgatgtgatg
     3241 actcgtattc tcaaggggga atttcaatga tgaatgaaaa aacaattgat ctactcatta
     3301 ttttattttt cacgttagtg attgtagcga aaagttatgt aatctttggc ttgttattcg
     3361 caattaccat gcttgtgctt tgcgtgttag caagcagaaa agaggttaaa catgaacgag
     3421 ttagcaaatc ttgacaacta tttaactgat cctgattatg ctaaaccacc atatgaagca
     3481 ccaattgatg aggaggatga agatgagtaa atctacctta gaaatgagcc atcaagaatg
     3541 gcttgaagac cgtaagaaag gcatcggagg ttcggatgtt ggaacaattt taggattgaa
     3601 taaatggaaa tctccttatc aactatggct agaaaaaaca ggacaagtag tacttgaaga
     3661 atcaggaagt gagcccgctt attggggtaa tgttttagaa gaagtggttg ctaaagagtt
     3721 tcaggaacgt acaggcaaaa aagtacgcag aagaaaccaa gtatttgaac atccattaca
     3781 tccattttta agagcaaata ttgatcggga agtagtggga gaaaatgcca ttctggaatg
     3841 caaaacagcc aatcaatttc tcggtaaaga atgggaaggc gaagaggtac cgcttagcta
     3901 tctctgtcaa gttcaacatt acatgaacgt tctaaacaaa gactattgtt acatcgctgt
     3961 cttaatcggt ggtcaaaaat ttatctggaa gcggattgaa cgagatcaag agctgatcga
     4021 tacaatcact gaacaattag tagagttttg ggaaacaaac gttcttggag gtatcgagcc
     4081 aattattgat ggtagtcaag cgacagctga tttcttgaaa gagaagtatg cagatgttga
     4141 agacgttcaa acgacgttac caattcattt cgatgaacta gttgaacaga aaaacgaact
     4201 caaacggact aagaaagaaa ttgaatcagc tatccgtcaa gtagacaacg agattatcag
     4261 cgagctaggt aaacgtgaag ccagtatcgg tatcactcaa aaaaacatca tcagttggaa
     4321 atttgtccgt acgagacgta tgaactcgaa gaaactagca gagaaatatc cagatgtcgc
     4381 aaatgatgaa gagatttata acgttactga atcaagaagg ctaaccgaaa gggagatcaa
     4441 ataatatggc aacaaatgaa tcgttaaaaa atcaattggc agcaaagcca cagaaacaag
     4501 ttgcaccagg tcaattagga cttaaagctt tgatgaatac accaacaatg agaaagaagt
     4561 tcgaagaggt gcttcatgac aatgctaatg cttttatgtc gaatgttatg actcttgtat
     4621 ctaatgacag ttatcttgca gatagtgaac cgatgtctat catgagtggt gcgttaactg
     4681 ctgcaacatt aaatcttggg ctagataaga atttaggtta tgcatattta gttccattca
     4741 atagtaaaaa caagcaaaca ggaaaatggg aaaagaaagc tcaattcatg cttggctata
     4801 aaggatatat ccaattagcc caacgatcag gtaaatacaa agcattaaat gtgatcgaag
     4861 tttacgaagg agaactaaaa agctggaacc gactgacaga agagtttgag tttgatccaa
     4921 atggtagaac gtctgatgaa gtcattggat atgttggcta tttcgagtta ctgaatggat
     4981 tcaaaaaaac tgtctattgg accaaacaag aaatcgaagc tcatcggatt gctaacaata
     5041 aagatcgcga taagacaaag ttaagtggtg tgtgggcatc tgattacaat gcaatggcac
     5101 gaaaaactgt tttgagaaat cttctttcta aatggggaat cttgtccatc gaaatgcaag
     5161 aagccaccac atcggatgag agagtccaaa gggttcaaga agacggcagc attattgctg
     5221 aaacagaagt tgaagaagat attcctgaaa gaaaagaagc agaggttata tctgaagaaa
     5281 atgaagatgt acaaactgga ttatttgatg catctaatcc gccgttaaac aaataatgag
     5341 ggagctttct cccttgcttt tctagaggag aaatacgaat gaacacagga tatgtcaaat
     5401 tacatcgaaa agtgatgaat tcattcgttt ggaccaatcc ttatatgtat aaattatgga
     5461 acttgtgttt gatgaaagcg agtcatgaga atcgcaagat actttttaat ggaaaagaga
     5521 tacaagtgaa cagcggagaa ttcgtcacag ggcgcgatgc tatcacatct gagatgaacg
     5581 aaggcgtgaa acgtgaacat caagtgaaca gcggttctgt atggagatgg ctaaaacaat
     5641 ttgaaaaaaa cggaatgttg aacatcaaat caactacgaa atacagcgtt atatcaataa
     5701 aaaactggtc tttgtaccaa agcagtgaac aacaaatgaa catcaaacgc acaacaagtg
     5761 aacaacaagt gcacacaaac aagaatgaaa agaattataa gaatgaaaag aataataaaa
     5821 catcatcgtc gcaaccacgc aaaaagcgtg tttacgacac cgactcagtt tactacattc
     5881 tcgcggagga gttattcaaa cagatttgtc agaatcagga aatcaaaaag ccgaatctgc
     5941 aaagttgggc agatgacatt cggaaaatga tcgagatcga caagcggacc gaaaaccagg
     6001 tacggggaat gattgaatgg agtcagcaca atgtgttttg ggcatcgaat attttatctg
     6061 ctaaaaaatt gcgagaaaaa tacgacacaa tggcagcgca agcaaatcgt gattataaaa
     6121 caaaacaaac taaaacgctt gaatacgaga aatttagcac agatgagttg cctatttgag
     6181 aggaggcgta ggcatggaaa ctgttgggga aatcatggaa aagctgatac agaaagtgct
     6241 tgtccaacgt ggcgaatgtc ctgaatgcgg acagcctttg tatggatggc gtacgaagaa
     6301 ccctgatggc tcagaacgtt gtaagccaac ctgtatgcaa tgtgggtata aagcattgcg
     6361 agttcaggaa gacttacaaa cagaacgaat ttacaacgag agcctgaaag caagagcaat
     6421 caattttttc aaaggtggtt ctgttgtgcc taatcaagcg ttgtttgatt gcacattgca
     6481 gaattatcaa attgtcgatc aagaaacaag acaagcggtt gaagtaacca aacgctttgt
     6541 taattcagtc ttgttaggaa atccaagtca ccttgtttta actggaaaac aaggaacagg
     6601 taaaagtcac ctagcgatgg cagcggcttg ggaagtcttg aagcgatcaa actacgataa
     6661 aaaaatctta tttatcgggt tacaggaaat gctggatcaa atcaaatttt cgtacaacaa
     6721 tcctgaactc agaaaaacga ttgagggatc gttgattgca gatatcaaaa cagcggattt
     6781 agtcatcatt gatgatatcg gttcagaact aggaaaagat gcatcagata gtcgagcgtt
     6841 tggcataaac acgctaaatt cgttcttgga tgcacgacag aacctagcaa cgattatcac
     6901 aacgaacttg cttggtgaag aactgaaaaa agcttacggt acgagaacaa tatcaagaat
     6961 gtttgtcaac tctgatggat ttacgatggt attttctcaa acagcagaca agcgcataaa
     7021 accagtcaaa ggagcgatcg catgaatttg actaaacaag aaaaaagaat ctgtcgtcag
     7081 ataatgttgc aaattatagg tgatcgtgac atggacgaat tatctcaaca agagagtcga
     7141 atggttcgga agataagtaa gctaattatt tatcgggata cagaaagatt tctcactgtt
     7201 ttaggtaacg tctacgagaa tccagagtta ttggaggtag agtaattgcc aaaaatatta
     7261 gatgcatgtt gcggtagtag attgttctgg tttgataaga acaattctga cgtaacattt
     7321 atggattgtc gtcagcagta tgaagaatta tcaacaggtc atgtgatcaa tgttgatcca
     7381 gatgtggtcg cagactttcg cgatatgcct tttgaagatg atgtgttcga tatggttgta
     7441 tttgacccgc cacacttaag acatgtcggc gaaaactcat ggcttgccaa aaaatatgga
     7501 aaattagacg aactttggcc agaagacatc aggcaaggtt ttgcagagtg tatgcgagtt
     7561 ttacgacctt caggaacatt aatttttaag tggaacgagg aacagatacc actttctgat
     7621 gtgttagagg ctattggaga acagccattg tttggtaata aacgaagcaa aacacattgg
     7681 cttgtgttta tgaaaaaatt cagctaacgg aggaaacaaa ggaggaaaag taatgaagat
     7741 tgtattaaat aaatgttttg gtggctttgg gttgagccat gaagcaaaaa tggaaatttt
     7801 taaaagaaaa aatattgaag tattcccata catcaataac tttagctatg actcagatga
     7861 tgaatacacg agatacacag gccaaaagct aggttcgatg gattttatct actatttcaa
     7921 aaaagacccg aaaatcgaca aagttacagg aatttatagc gaaattgacc gactgtacgg
     7981 aattgctgat gattccagtt tttcaagtga ttcaaacaga ggtgataagg acctcgttgc
     8041 tgttgtcgaa aaactaggaa gtgaggccag tggaccatat gcaagtctta aagtcgttga
     8101 tatacccgat ggggctgagt gggaaataag tgattatgac ggcgttgaaa ctgcacatta
     8161 tggttttcaa acaggtagta tttagtccac taatgacaac taaagaggag gcagaaggat
     8221 gagtgaagat gaatactttg aacagtagtc tattaacgac agagattttt aactggaaag
     8281 gggtgtagtc atggacgcat cggaagtagt aaataaaatt aatagtctca aagaggtttt
     8341 cggagacgtc gaagtagtga taaacgcaca aggctatgac aacgaatttt ttaagcagat
     8401 taccgacatt agcattcagc aaggaatgga agacgaagac ggaaacttta tcgatgaagt
     8461 agccatttta gtgacttgcg agtaagtaat tgacagctaa agaggaggaa gcggaatgaa
     8521 actaaaagac ggattttact ccagcggcca cggcatcggc ggtttaatgc tagatatgcc
     8581 gacaaagaat cctaaaacac gtaagaaacc aaaattcaaa gtcggtgaca tggttcgctg
     8641 cgaagcagaa ggattcatct atccatttcg tggatatgta gaacacgtct ataatcactc
     8701 agcaatcatt cgcattgaaa acacgatgga atgtgataag tggttagcga aaagcaaaga
     8761 gaatttagca gtggtgagat tggtggacat ggaagttata aaatcttaat tttgcttttt
     8821 tacaactatc ctacaacaat gagcgttatt ccacaactgg aaggagaaga ggcaattgtg
     8881 aacattttag agaatataga tattaagcaa acaaggaaaa atgctagacg attactaaaa
     8941 agatacagaa atttagaacg tttggtcgga ccagtgaaaa tagacttttc cgtgatgact
     9001 gttaccaaaa atttgaaatt cacaattgac agtcaaaacg aagaaatcat tgaagcgata
     9061 agtactcgag attcggttat cgaagcatta acacggctaa gtagaatcca tttccaggta
     9121 ctttattata gttattgttt tcctaataag atgtcaatgt atcaaatagg agaaaaatta
     9181 ggatattccg atagaactat cgagagaatg aaggcagtag ctttggttga atttgctgag
     9241 gcgtataaat caggagaact catttcacgt acaaaataaa aaaagtcgga ttcctccgac
     9301 tatgaataat atttccgaca taagtattat atcataattg ggggaatcag aggatggtac
     9361 ttttcgatgt aaagaaatat gagacaccaa gcgcgaaaga tgttgatatg gaacgcacaa
     9421 aacataatgt cgctgtgttt ctttcagcat atctatcagc tagatgtaga gtagggcaac
     9481 atcgagaacc aaaagtgaca gcatcttatt ccttggttcc accttctaca gctgatcata
     9541 tatttgaagc agaaagaatg ctgatcgata aagaagaagc acaggaagaa tttgagtatc
     9601 tgcataaatt gtttgttcga ggctattcag cgatacagca tccgcataag cctgatgtga
     9661 ctgaaaggcg caagaagata ttctatgatc gttatatcaa tggtctgccc atttatgtaa
     9721 ctgctcaaag gaataatact agcgaagaat cggttaaagt agaatcaaac agaattatca
     9781 tccaatttgc ttcatcgtta gaactggttg ctttcaagta gccagttttt acacttttta
     9841 taccctttta ttaccaattt ggtttccatt ttataccttt tttataccaa tcacttacct
     9901 attcaacgtt gtattatgat agtgtcgaaa aattaggaaa caggatcgac aaaataaaat
     9961 gtaagggagg aaatctccct catcgtttaa ttaagcttcg ataggcagca acggaaatat
    10021 taagaataag gatgtgaatt tcaactcctt ctaaattgtt cttattatct atcatccgtt
    10081 gctgtctatt gtttttttaa ttattcacac gataactaaa ggtaagtgaa gagaaatgat
    10141 tccattaata atttcaattt ttgcgctttg tcttaatgta tatatgattg gatttaaaaa
    10201 tgggcaaaat aaaaaatagt agcagccaag aataatttta tagtgtcact gtggcggaaa
    10261 gggtagacgc taagcatgtg tgctaggtca atgcttcggc aaccatgcaa ggttcgattc
    10321 cttgccagtg actttggcaa ccgaggcatc ggcggtttaa aaatataggg gtgcgcaatt
    10381 tcgtacgcgt tttgtgcatc gtgtaggttg ctattacata actggttagg ttagattgag
    10441 atttgggatt cggtacaaat gaatcgtcaa atgtctcaag cacaggatcg gaaacgtccc
    10501 tgcctgtgca ttacatatta gatcactctt tgagtggtct ttttctttac ataaaggagg
    10561 ctacataatg agaaactact ggtatgtatc gctaactaat gaatatcctc gaaccattga
    10621 tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact ctattgtcga
    10681 aatggggaga gaagcagaac catgtgagat tgatgcgtgc aagctcgttt attgcggtca
    10741 tggtttctat gatgaaccaa acattcaaaa taatattaac aagaatttga gggattagaa
    10801 tgcaagaaat ggcttaaaaa tctccaatca ggacaaatca agttagacaa agtttccgat
    10861 ttaaagatat tgattgaagc tgatttaatg ctaaaagata ttgaaaatta gaaaacaaaa
    10921 ctcaacctaa acagaatgtg aggtggtgtg tattgaatgg caagacaacg tgatccaaga
    10981 cgtgatgaag ccaaaagaat ttggttagaa tccaacggag aaaagcagtt aaaggaaatt
    11041 gcatctgaat taaatgtttc agattctcag gttagaaaat ggaaatcgca agacaaatgg
    11101 agcgctgaat tgattagtaa cgttaccaat ggcaaaagta acgttactaa tcaaggtggc
    11161 gctcctattg gtaatcaaaa tgctaaaggt aacaaaggaa atagccgagc ttctccgcca
    11221 gtgggtaata aaaacgcttt gaaaacaggt gagtatgaaa caatattttt tgacacgtta
    11281 accgataatg agaaggacat ctattctagt ttgaatgatg atccttcttt tgctttgtcc
    11341 gaagaaatac gacttcttaa aataagacaa cttcgaatga tgaaaagaat ccaacaagct
    11401 gaagctggac taaatgatga agaagtcgaa cgattgcagc agctaagaaa gattaaaact
    11461 ccgattgaaa aaaatggtaa aaagctagaa atcaagcgtg aggttatgca agatgtgcag
    11521 attagcagaa aaaaacatcg caaaattgat gatattcttt caattgaaga ttcattgact
    11581 cggattagca accagttagc taaagccatc aagcaaatga atgaacttta tatgaatgaa
    11641 tacagaactg atttaattaa agctcagact gataagatcc aagctgagac aaatgaaatt
    11701 ggcggaaata attcaggtga agaaatagaa gaatggaaac aggcagtttt aaatgccgca
    11761 aacaaacggg cggtgaaaga aaatgaataa tgaatttatt ccttttgctg atattggttc
    11821 tgccattgat tattactatg ataaaccagt agctttttgc caagatattt tgcatttgaa
    11881 tcctgatgaa tggcaagaaa atgttttaaa tgatttagct gaattttcaa aggtttctgt
    11941 tcgttctggt caaggagttg gaaaaacagc attagaagca ggagcaatac tttggttctt
    12001 aacgtgtcga ccctacgcta aagtaatagc aacagctccg acaatgaagc aactttacga
    12061 tgtactttgg gcagaggtag ctaaatggtt aaatgatagc ttgatcaaaa acttactgaa
    12121 gtggacaaag accaaaattt atatggttgg tgattcagag cgttggtttg ctacggctag
    12181 aacagcgact aaaccagaaa gtatgcaagg ttttcacgag gaccatatgt tgattgtggt
    12241 agatgaagct tctggtgtgt ctgatccaat tatggaagct attcttggta cgctatcagg
    12301 ttttgataat aagctgttga tgtgtggaaa ccccaataat attgaaggtg tcttttatga
    12361 ttcccataat tcagaccgtg ataaatacag agttcataaa gtatcaagct atgatagtaa
    12421 acgtacaaac aaagacaata tagaaatgat tcttaaaaaa tatggaaaag aaagtgatgt
    12481 tgctcgtgtc cgtatttttg gagaatttcc gaaaggtgcg ttggattcat ttatcagtct
    12541 tgaaacagtt gaattggcta cagaaaaaca aattagtgat tctttagtca ataaaacaaa
    12601 ggttgctcat attggtgtcg acgtagctcg atatggtgat gattctacga ttctctttcc
    12661 tagaattgct accagggcat tggagtatga gaagtattca aaacgtagca ccatggaaac
    12721 aacaggatat gtcatcaaca tggccaagaa tctaatgagt caatatccga gtattgataa
    12781 agtgatgatt aaagtcgatg acactggtgt cggaggtggt gtaaccgacc gcctagaaga
    12841 acttatagaa gacaaacatt atccttttga ggtgtttgga gtgaataacg gttcaacatc
    12901 agaagacgat ttttacgata atttaggtac tcaactatgg ggaaacatca aggaaatgtt
    12961 agaagaaaat atgacagcaa atcttaacgg agaacagcct gttattgaat tgccttctga
    13021 tagttcgtta atcaaagaat taagtactcg caaattcaaa atgacaagta gaagtcgtat
    13081 acgtttagaa agtaaagatg atatgaaaaa gcgaaatatt ggtagtcccg atattgctga
    13141 cgcactggct ttagcgtttt atgagccacc aagtcactat caatttattc aattttagga
    13201 ggtgagcttt tgattagtac agtattatct ttagagagat ataagaagct acgtgttaaa
    13261 tatgcaacac aaattgaaga tggaatgttt gatccgaatg gttttataga agatatgaaa
    13321 ccattttttg ctgatcgcga aagaaaatat ctagcttata ctagcgaaaa gaatgaaata
    13381 gataatagac caaagcctaa cactgatatt gtaaaagtta ataataaact tcatgctggt
    13441 atgtattcaa ttgtcgtaga ccaagcagtc aatcatttta ctggtatacc tatcaaatgg
    13501 gattacgatg tatcggaaca aaaaagaaca ctcattcaaa gattaaaaga taaattctta
    13561 aaaaatgata tagaacttcc gacagttcca gaagctttta acaaattaac aagcaatctt
    13621 gattccatga gattcgcaat gcttgattct gaaactgcaa cttttcaagg agcttgtgga
    13681 gtagctttcc gattgctaga gcctgtggaa gaagatgatg gttggaagtt aagagcaagt
    13741 aatattgaac cttggagagc agaaagatat ggaaatgccg gaatctatat caaagaaaag
    13801 tatgactctt accagaaaaa attttttcaa gaaatgaaag ttatgacaag aaataaaatt
    13861 ctgacatatg cttgctatgg tgatttgaat tttgttacaa gcggaacgtt caaaaaaatt
    13921 gatgagaccg ataacccttt ggggacgatt accttggcag aatttaaaaa taatacgaat
    13981 cgttattgtg attttgaagt agctgaggaa attggagatg caattgatcg tgctttgtct
    14041 gatcaacaaa acgaaatcga acaatttaag cttgcgtaca tgcttgttac tggcacaaca
    14101 atgagcaaag gcactgctaa agaaatgatg aatcagctag ggattatcaa cttaaaagcc
    14161 cctactgcaa aagctgaata tgtcacaaaa aatttagcaa aagatttcaa tgaatatcat
    14221 atggatctat tgaaaaaaca gttttacacc atttgtaaag caatcgattt taacgatgag
    14281 gtgtttaaat caaacagttc tggagaagct cgcaagtggc aaatcattag cctagaagcg
    14341 aaaacaaata ctaaagagca atattttaga gaaggattga aagaatgcgc agaaacaatt
    14401 gctgcttttc ttaagtttca tgacaaagta gaaattgagc ctgaaaaaat tatttttact
    14461 ttctctagat ctttgccaac tgatttaagt tatttagctg aagctttacc taaacttgca
    14521 ccatacgtat ccaaacgaac tattcagagc caaattccat ttgtaacaga tgttgattat
    14581 gaaaatgaaa tgatggaatt ggaaagtgga agtgcttatc cagatagtga atacaatttt
    14641 ggcggaggtg gcaatagtga cgatagaaac gaagtattgg accaaacgtc gagaactgga
    14701 ggatcaagca aggctcaaac aagaaaatca gacacttaaa aaattaacta gtgtatttcc
    14761 tgaagcactg aaagagatac aagcaaaact attatcacaa gctgacttac acaatatcac
    14821 ttatccagaa atgatggagt tttacagtac aagtaatcag aaaaaatatc gtgaatatgt
    14881 ggaaaaaaat tataagtcat taaaaatgta tgatgcaaaa tacaaagagt ttatcgatga
    14941 attttttcca ccatttgact atgcaaaagt caatcgctta ttacaaatac gatcagatgt
    15001 atttaaaatt cttgcagaat atgcgatgga tgcagatgtg aatcaatatt tttctgatcg
    15061 cttagaggaa attcttcaaa gaacatattc ttctaatgct aatgtttttg ttcaactttt
    15121 aaacgttgat ataccaaatt atttaccaga aaatgaaatt aaacattatt tgaactatcc
    15181 atggtcagga aagacatttt caagaagact ttggggaaat atttcatcgc ttgaacagaa
    15241 actctctaat gctattgtaa aaagtgttgc tagtggagaa ggcgttatac acgcattaaa
    15301 caccatgaga ttagactcag aaatttgtga catgtttaag ttagaagaat caaagtataa
    15361 caaagcgata gaaaatctcg ttcgaacgga gtatgcaaaa tttgcacaag atggtattga
    15421 aaaatcatat ttagaaacag gtattgagga atacaacgta ttgactgcaa aagatgagag
    15481 agtttgccgg atttgtggag gaaaggcaag taagaatccc tataaactga aagatgctgt
    15541 cataggtgaa aatcgagcac ctttccatag tcgttgtaga tgtacggatg ttcctaattt
    15601 accaaaatta ggaaaggata ttgatgaaga atatgaccgt ttatttggcg atttattaga
    15661 tgagtttgca catgattctt ttggaattaa tttgaaacgg aggaagtaga atgaaagatt
    15721 tttttgaagc agtactaaca attaatgtaa atgctgatat tgcagaagcc tacaaaacag
    15781 ctattgagtc tgagaaccat cctaatggct tgagagacca ttggaatggc aattatgcct
    15841 acgtggttat tggcgatcaa actgttaatt atcaagataa tactccagtt gataagaata
    15901 ccgttaattt aacgattcaa ttattatctc attcattacc aaatttaaaa gaaacagttg
    15961 attggtatga aaagatggga tgtattgttg ttagaactga ctacaaagaa ggaaagtcta
    16021 gtaattaggc ttttttattt tgtccgaaat gacgttaaac tagcgcaata ctgggcttgg
    16081 ttgaatggtg gggcgcaact attaaaactc aaagcaatgc ggggcgtgaa aacgaatcgt
    16141 ggggcgaaag gagaatgagc atgaaacaca aatcattaat gccaatgaat ttgcaatact
    16201 ttgccgaagg tgacgatcaa aagttttctt ttgatgactt caaatctttt gtggaatcca
    16261 atgaagaagc tcaaaaattt attcagtcac aatctcaaag tgttgccgac aaacaattgg
    16321 aagcttggaa acaaaataac ttagataaaa ttaaacagga taccatcaag gaatatgagg
    16381 aatctaagaa aaacaagtca cctgagcaaa ttcaactgga aaaactacaa gctgaatttg
    16441 aagcagaaaa agcgttgcgt gtgacaagtg ataataaagc atttgttgca gaacaaattg
    16501 ctggattaga actagatgga gagttaaaag agtctatttc tcaatttatg ctaaataatc
    16561 ttgttagttc ggatacagat ttcactaaga atgctgttga aggttttaca agtgttttga
    16621 atgcaattaa tgagaaacat gcagatgcat taaaagaact acaaatgaag tctgcatttg
    16681 gtggaactca acaatcgaat aaccaggttc agcagaacaa tgaaacatta acaaatccag
    16741 aagaacaatt aggacaaatt cttcaacaat ttaactagga gagtgaaaaa ttatgaaaaa
    16801 aacatcttta aataatttag agtatttgga tatttcacca gcgattaatg ctatgcaagt
    16861 accaaataca ccttttttaa gctatttact tggtgctgga aaaacagagc cagcaaactc
    16921 gacagaaatt aaatggcgtg aatatgatat caacaacgat gattcttctg aaaaacttga
    16981 gggcggagaa tatccagatg ctgaatcagg tcgaacttgg tttaacaact atactgaaat
    17041 ttttagaaaa tcaacctctg tatctggtac attagatgct attaatgtga atggagtcgg
    17101 aaatgaatta actaatcaag tagcactacg tggtatggaa atgaaaattg atttgaaccg
    17161 aaaattgatt actggtgtaa aagctgatga aaatggttct aaaggtcgtc gaatgaatgg
    17221 gattttgaac ttgatcaatt cagcaaataa ggcagaaaca gccactgcgg gtgcagtaac
    17281 aagaaaagat atcgatactt tatttaaatt gatgtatgaa aaaggttata tgggagaaaa
    17341 actatgcttg atttctccag atatgcagga gttaatgact gatgagttag atggaaaatc
    17401 aacaaaaatt gttcagttcg gggaaagagt aacttttgga ttgcaaattg gaaatatcgt
    17461 gtctaattac ggtacaggta ttgctctact agaaccatca ttgccaaaag gaacaattgc
    17521 cgcaatcgat actaattatg tgaaactacg tccattacgt gaatggagag cagaagaact
    17581 tgcaaaaaca acggattcta gacgtatcgg tcttgtaggt gaatactctc ttgaatacaa
    17641 tgcttcaaac tctggggcaa ttttaaattt aaagtctgaa taaaagggag ttagtactcc
    17701 cttttttgat aggaggaatt acagtggtaa aaaaagatga aactaaaaaa gatgaagtcg
    17761 tgaaatatag agtaggtaaa actaaaaatt ttgttgggtt tgttcatcct aaaactcgta
    17821 gatttatcac agcagattca aataacgaat ttatcatttc tatagatgat aaagaagcaa
    17881 ttgcaatttt ggaagatgca attgatgtta atgaaattta ggaagtgatc tgatggatga
    17941 atcgctaaaa acggaaatca ttgagtctac aaaagaagat tttccagatt tgagtgaaga
    18001 acgcataact aatttattag aaataatttt gctagaaatt gaatcataca acacttgtaa
    18061 aaatgatatt tcatgggaaa agttaaaaag cgtgattagt gaagtgttgt ataaaataat
    18121 aaaaaatgaa tcagaaaaaa cagtatcttc agttagacgt ggcgatacaa cgattagtta
    18181 tgcttcaaca acaaatgatg ttagtgagtt acttctaggc tatggtgatt tgatacgaag
    18241 agttattggt tgtggaggat tggagttttt ttaatgaatg aagcagatat tttagaaact
    18301 acttatgaag atagttgtat tattgaaaga ctgacggaca ttgaagattc taatacaaat
    18361 attactattc aagattacaa aaaagtatat gataatccta tttcttgcgc tctttcacaa
    18421 ggtcaaatcg atggactagc agtcatagaa gatggagaga tggtaaatgt ttcaactgac
    18481 acatataaat tatttgttca tcctaagatt aaactaaaaa aaggagatcg aataacaata
    18541 actcaaaagg ccagtggctt aattttttct ctatttgcta ctaagccttt ttactatcct
    18601 agtcattgtg aagtgaattt gataggaagt gagaaaaatg ggtaatctca aatatgaatc
    18661 aaatgcagaa aagatcattg agaattttaa aaatatgact gtaattgctc aaaaagaagg
    18721 aatatccttc gttaacgatt caatgaataa agtcgttagt ttagctaaac cattaactcc
    18781 tgtaaaatca ggtaatttaa ggcgaggata tcgtgtagta aaagctagaa aactatcaag
    18841 cggtcgaatt gttggagaag ttataaacaa tgaacattat tttaaatatg tagaagaagg
    18901 tcataggact aaaaatggtg gctttgtcaa agggaaattc atgttaactc gtgcaacgaa
    18961 tcttgcaaat atgtcttata ttcctcgaag atttaaacaa atggcaataa aaatcgttaa
    19021 gaaaggaaag taacatgaaa gatgaaatca ttgctgcaat cagtagcaaa ttaaaagaaa
    19081 tctatcctga tgggacaata tatcttgatt cagttatgca gtcaactaaa gatttttatt
    19141 tcgttttatc agtaatggaa tctggaactg aaaatgtagg aattgatatt caaaatgttt
    19201 ctttcttaat tgatattgca ttgattgata ataaccctaa tagaaattta attaatgaat
    19261 tagtctcacg ttgtgggact ttttttaata cgattacaat cgatgagcaa atattatttc
    19321 caaaagccta tttacctgat gaagcagatg gtgttcaaca tattcgtttt acattagaat
    19381 ttccacaata tattgaatgg agtgaaagat aaatgggaga aaaaagaagt aaaaccggaa
    19441 ttatttctgt cgaaaaacct acttggtttc ctttggaaga tgagacaggg aattttccgg
    19501 tatatggtac tgcgttgcca atgggaacag cagtaagtat caaaccaaca gctaattatg
    19561 aaacaacaca agattatggg gattcagtgg ttcaagatca atttacagct tttggtggtg
    19621 cagaagttga actggaagca aatggatata ctcataaagt tttatctaca attactggag
    19681 ggaaaattgt taaaggtggt gccttgcgtt ctggagaaga tattgcgcaa gatggtgcgt
    19741 ttgcatatag acgtaaaaaa tcgaatggga aataccgtta tacggtcttt tataaaggac
    19801 agtttgctct agattcagat gaaacatcaa cgattgaagg aagtaaagta agttttactc
    19861 atccaacttg gaagggttca tttgtagatg taccaggact tggatatatg tattcagttg
    19921 atgaagatga tgaaggcgtg gataaagcaa tgatcggaaa ttggtttaca aaagtagcaa
    19981 ttccaattga agaagcagaa ctttcaggag gtacagaata atgtcaaaat atcaaacaac
    20041 gattaaatta acgaaaaaag acgaagaagg taaatatgag caagtacaat ttaaatctgc
    20101 tgaattttta ccaggaacag ttgtagaaga tgctgcagga gttatggaag aaatgcaaac
    20161 agcgactgat aagcaatcag ttaaaaaagc tttaagtcgt gcttattcat ttattgcaga
    20221 tactttattt gagggacaat ttactggtga agattattgc aaaggaattg atgctcgtga
    20281 gattgcctca ttgacaggaa aattattgaa gtctgttact gcaggttttg atgaaactta
    20341 tacagagacg aaaaaaaagt aagtgaggcg ctcaagtcac cttcatttaa gtattcgatt
    20401 acttaccgag aattagatat aaagacgcaa ttactcgaag caggttggac gttaccagaa
    20461 attgaacgta ctgacttgga tgagttaatg cgtctttttg cttttagaga tgcggtaaaa
    20521 gagcatgaag atgttgagta ctatgataat ttcactcaat tttaggaggt gatactttga
    20581 ataacgatga cctaattctg aagatgatac tagatgaatc tggttttact gccggtatga
    20641 ataatgctgt caaaaagttg aactcttttg atgaaacaat cgaaagaaaa agtagaaata
    20701 gtggcagttc attaggtagt atttggaaaa tatttgctgg aagttttcta gctagcggag
    20761 taactagaat cgtaggagct ggctttgatc taatcaaggg ttccataagt ggagcggttg
    20821 atcgagtaga tacgatgaat aacgctctac gaaatttcca aaatatggga tttagtaatt
    20881 cagaaattat gaagaatatc ggaaagaatg ggcttttatc tcaaggtatt caaggacttc
    20941 ctactgcttt gaatgatgcg ataagtcatg ttcaacttct tgcttcttct acaggtgatt
    21001 taactcgttc aactcaaata tttaaagctt taaatgatgg aattcttggt ttcggtggtt
    21061 caactgatca agttaatgaa gctgttattc aattatctca aagtttctca aacggaaaag
    21121 tagacgcaca aacttggaac tcaatgatca acgctcaact tggacctact ctttctgcta
    21181 ttgctaaaaa gatgggaatt acaatgggag atctgaaaga aggtttatct caaggtaaga
    21241 tttctgttga agaattccaa aatcaattaa tagaaatgga taccaaaggt ggcggaggac
    21301 ttaaatcatt aagtcaaatc gctaaagatt ctactaaggg aattaaaacc tctatacaaa
    21361 atgcaaaaac agctgttaca cgtggtgttg gtgaggtaat agaaggatta aataaagcat
    21421 tagtagattc agatttaggt ggatttaagg gaattattga taaagtgggc agttcaatgg
    21481 aatcgttctt aaaagtaatt gctgcaaata ttcctacagc agtatcattt ttaagtaatt
    21541 tatttaacga aattcaaaaa tttggttctg ctttgaaatt catgatgccc tttcttgttc
    21601 ctgcagctac cgcttttgga gcgttcatgt ttcaacttaa aggtatacca gcaataataa
    21661 aaagcttcaa taattttaag aatgccataa ttggcgttgg aaattcatta aagattatgg
    21721 gggcaatagc tgctgctaat ccatttgttt taattattgg tgcagtcgtt ggtgcaatag
    21781 ctgttttcgg ttactttatg gcaaccaatg aagagcttag aaacaaggtt atatctattt
    21841 ggaatgatgt gaaagattcc gtaattggtg tattaaaaaa tataaaggac tggggaattg
    21901 atacttggaa ttctgctaaa gaaatggcat caaatgcagt tgagggtgtc aaagatgctt
    21961 ggtcaggaac aaaggaatgg ttttctaaca cctggaaagg tattaaggat ggggccacag
    22021 gtttatttga taaaacagta gagacttcaa gaaatgcagt cgatagtgta aaaaatgcat
    22081 ggtctaatat gaagaaatgg ttttctgata cttggcaaag tataaaagat tcagccagag
    22141 aaaaatggaa tggaatcaaa ggttctatta tgtcagttgc tggtccattg attacaggta
    22201 taaagaatgc atttttacat gttacttttt atctagagac cttatggaat aatttagttg
    22261 agattggtaa aaacgtcttt gagattttaa aaaatgttat acttgcgccg gttttgttta
    22321 ttacctccat gatttctggt ggatgggaag aaacaaaaaa taatatgatt gctgtttgga
    22381 ataatattaa agaaagtgcc ataaatattt gggaatctat aaagaatata tttgtaagtt
    22441 attttacaaa tatttatttt gctgcactca atatttggac agggttcaag cttacattga
    22501 taaatatttg gaatgaagtg gtaaatcaag ctaaatcgat ttggttaaac ataaagtatt
    22561 tttttattaa tctttg
//
LOCUS       DAZUMT010000045        21992 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000045 DAZUMT010000000
VERSION     DAZUMT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.045"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..1166
                     /locus_tag="ACG8FM_001526"
     CDS             201..1166
                     /locus_tag="ACG8FM_001526"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5094193.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1265..2437)
                     /locus_tag="ACG8FM_001527"
     CDS             complement(1265..2437)
                     /locus_tag="ACG8FM_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5094194.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2424..3569)
                     /locus_tag="ACG8FM_001528"
     CDS             complement(2424..3569)
                     /locus_tag="ACG8FM_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5094195.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3583..4507)
                     /locus_tag="ACG8FM_001529"
                     /pseudo
     CDS             complement(3583..4507)
                     /locus_tag="ACG8FM_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4624..5703
                     /locus_tag="ACG8FM_001530"
     CDS             4624..5703
                     /locus_tag="ACG8FM_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5094196.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5700..6328
                     /locus_tag="ACG8FM_001531"
                     /pseudo
     CDS             5700..6328
                     /locus_tag="ACG8FM_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6438..6665)
                     /locus_tag="ACG8FM_001532"
     CDS             complement(6438..6665)
                     /locus_tag="ACG8FM_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5094197.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6768..9356)
                     /locus_tag="ACG8FM_001533"
     CDS             complement(6768..9356)
                     /locus_tag="ACG8FM_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS5094198.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9376..10035)
                     /locus_tag="ACG8FM_001534"
     CDS             complement(9376..10035)
                     /locus_tag="ACG8FM_001534"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5094199.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10084..10587)
                     /gene="trmL"
                     /locus_tag="ACG8FM_001535"
     CDS             complement(10084..10587)
                     /gene="trmL"
                     /locus_tag="ACG8FM_001535"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS5094200.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10808..11674)
                     /locus_tag="ACG8FM_001536"
     CDS             complement(10808..11674)
                     /locus_tag="ACG8FM_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5094201.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11783..12424
                     /locus_tag="ACG8FM_001537"
     CDS             11783..12424
                     /locus_tag="ACG8FM_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS5094202.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12486..13847)
                     /gene="mgtE"
                     /locus_tag="ACG8FM_001538"
     CDS             complement(12486..13847)
                     /gene="mgtE"
                     /locus_tag="ACG8FM_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS5094203.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13865..14764)
                     /locus_tag="ACG8FM_001539"
     CDS             complement(13865..14764)
                     /locus_tag="ACG8FM_001539"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5094204.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14766..15563)
                     /locus_tag="ACG8FM_001540"
     CDS             complement(14766..15563)
                     /locus_tag="ACG8FM_001540"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS5094205.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15541..16245)
                     /locus_tag="ACG8FM_001541"
     CDS             complement(15541..16245)
                     /locus_tag="ACG8FM_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS5094206.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16376..16951
                     /locus_tag="ACG8FM_001542"
     CDS             16376..16951
                     /locus_tag="ACG8FM_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS5094207.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            17069..17746
                     /locus_tag="ACG8FM_001543"
     CDS             17069..17746
                     /locus_tag="ACG8FM_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS5094208.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(17825..19633)
                     /gene="pepF"
                     /locus_tag="ACG8FM_001544"
     CDS             complement(17825..19633)
                     /gene="pepF"
                     /locus_tag="ACG8FM_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS5094209.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(19809..20807)
                     /locus_tag="ACG8FM_001545"
     CDS             complement(19809..20807)
                     /locus_tag="ACG8FM_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS5094210.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(21033..21686)
                     /locus_tag="ACG8FM_001546"
     CDS             complement(21033..21686)
                     /locus_tag="ACG8FM_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS5094211.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         6492 a         4698 c         3814 g         6988 t
ORIGIN      
        1 ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg ctataataac
       61 caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat tcaaaaaata
      121 gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata tcttgtttta
      181 ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat ccatcggcag
      241 gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa aataagcata
      301 ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt gctgaaactt
      361 tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc gatactttcc
      421 cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat actttctatc
      481 agatggaagt agaattccca gttgcttata ttccagcagg ttctggagat gattttgctc
      541 gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta gcagctcaat
      601 caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag
      661 ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca aatcattctt
      721 ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta ttctctattc
      781 tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga ggaaaacaat
      841 taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt ggcggaggaa
      901 tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac
      961 gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa aaacaattga
     1021 aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca acaacaccgc
     1081 aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc acaataacca
     1141 caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta catctggaac
     1201 accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt tttttactat
     1261 gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta cccaaaataa
     1321 attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg aaccaaaaag
     1381 attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta ttttgaacaa
     1441 accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag tattctgctg
     1501 gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg atcccaccgc
     1561 tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg atcggcaata
     1621 aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag gtgtggatca
     1681 ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa ctaatcaaac
     1741 taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg tttcgtttac
     1801 tgatctcttt gcgattaaat gctagattga tgacgctgaa accgctaaaa atggatagaa
     1861 acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc atccgctgac
     1921 tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt gtctgttcca
     1981 tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt
     2041 tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt atcttgatcg
     2101 tcggcatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt aagataatgt
     2161 ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca tccaatgtaa
     2221 cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc gactcttctt
     2281 cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca atattttgcg
     2341 caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc gatttatttg
     2401 ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct ttccatccaa
     2461 gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt ggtataaaaa
     2521 ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa gttgacgaga
     2581 tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg ttccgacccc
     2641 atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct atccttttgc
     2701 accagattca tttttggtat caaattacca accaatgtac ctaacaaaat cgcacacaac
     2761 gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc tacttgatag
     2821 ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt aaagcttgct
     2881 gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca
     2941 tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag
     3001 agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct
     3061 gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca
     3121 acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc
     3181 atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat
     3241 gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct
     3301 acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag
     3361 actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta
     3421 atcgtttcga gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct
     3481 aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt
     3541 gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt
     3601 gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta
     3661 actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt
     3721 agttgttact tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg
     3781 acaacacgtt ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca
     3841 tatcttttag agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga
     3901 tctggtcgca aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc
     3961 cttgtcgatt cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag
     4021 ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt
     4081 tcaaccgacg tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta
     4141 atcccactaa ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat
     4201 ataaaccgca aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt
     4261 cttgaggaac gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc
     4321 cgaacaattt aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag
     4381 tcgatttccc actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca
     4441 gatcaaaatg attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga
     4501 taatcatcat gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag
     4561 aaaagaaact agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct
     4621 gctatgcata aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt
     4681 ttgctggtaa gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta
     4741 ttcactggtg tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta
     4801 tttcttttat gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg
     4861 atcgcccctg aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg
     4921 acggttggta atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc
     4981 ttttacttac gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc
     5041 aaagatactc attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg
     5101 tcacaagaaa cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac
     5161 atccatgaca atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa
     5221 atgatcaatc aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac
     5281 actgggatgg atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt
     5341 caacactcct tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac
     5401 ttgtttgaag gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc
     5461 ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg
     5521 ccggcctttt atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct
     5581 cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat
     5641 attttccaag aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa
     5701 tgataacagt agtcattatt gagatgatcc atttgtgacg aaatcgttac aaaccatttt
     5761 agaaagcacg aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt
     5821 cttatacgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac
     5881 gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt
     5941 gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta
     6001 tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca
     6061 ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac
     6121 gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt
     6181 agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt
     6241 tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc
     6301 tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg
     6361 ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt
     6421 cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc
     6481 gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga
     6541 tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac
     6601 tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta
     6661 gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa
     6721 gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt
     6781 gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg
     6841 ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct
     6901 ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg
     6961 tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt
     7021 ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa
     7081 gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc
     7141 aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc
     7201 cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga
     7261 agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt
     7321 gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa
     7381 gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt
     7441 gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta
     7501 ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc
     7561 ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc
     7621 cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta
     7681 tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg
     7741 aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc
     7801 tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc
     7861 attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag
     7921 ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat
     7981 aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct
     8041 tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga
     8101 tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt
     8161 ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt
     8221 ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct
     8281 ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc
     8341 gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct
     8401 ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc
     8461 ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt
     8521 ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat ttcaattggg
     8581 cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta
     8641 tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc
     8701 ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt
     8761 ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc
     8821 ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc
     8881 atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt
     8941 ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata
     9001 gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat
     9061 ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat
     9121 ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga
     9181 taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg
     9241 taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt
     9301 tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga
     9361 agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt
     9421 ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat
     9481 cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt
     9541 catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc
     9601 tttatgttgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat
     9661 cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag
     9721 attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc
     9781 tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat
     9841 accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac
     9901 atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga
     9961 atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt
    10021 aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac
    10081 tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg
    10141 ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac
    10201 atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc
    10261 tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt
    10321 ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa
    10381 aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc
    10441 caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg
    10501 aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa
    10561 tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc
    10621 tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact
    10681 gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag gaacctgata
    10741 aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct
    10801 ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt
    10861 caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct
    10921 gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt
    10981 cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac
    11041 gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct
    11101 ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt
    11161 ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag
    11221 caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag
    11281 ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt
    11341 ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt
    11401 caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc
    11461 tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat
    11521 aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat
    11581 cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca
    11641 aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct
    11701 taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt
    11761 gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat
    11821 tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg
    11881 tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga
    11941 tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga
    12001 attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt
    12061 atttggttgt cttactcctt caggctggct agacgaagag gccttagaac tattgatcga
    12121 aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg
    12181 gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg
    12241 cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta
    12301 tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca
    12361 tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt
    12421 ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg
    12481 tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc
    12541 gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga
    12601 cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc
    12661 atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga
    12721 aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca
    12781 agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct
    12841 agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa
    12901 atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacatacct
    12961 aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca
    13021 ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca
    13081 ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta
    13141 acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg
    13201 cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca
    13261 ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca
    13321 tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag
    13381 cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt
    13441 tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt
    13501 aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta
    13561 tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc
    13621 atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga
    13681 taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca
    13741 tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct
    13801 tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct
    13861 cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga
    13921 actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt
    13981 gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc
    14041 caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa
    14101 ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt
    14161 catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat
    14221 gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa
    14281 acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa
    14341 gcatcaaacc agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt
    14401 aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt
    14461 gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat
    14521 acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa
    14581 tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat
    14641 taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt
    14701 tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact
    14761 gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga
    14821 taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct
    14881 tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt
    14941 tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta
    15001 ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg
    15061 ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct
    15121 ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc
    15181 cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa
    15241 ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg
    15301 tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta
    15361 tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat
    15421 aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc
    15481 cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta
    15541 ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag
    15601 cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc
    15661 ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc
    15721 acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga
    15781 atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat
    15841 tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta
    15901 gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct
    15961 tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa
    16021 cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga
    16081 ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg
    16141 tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac
    16201 agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac
    16261 ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt
    16321 atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac
    16381 acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat
    16441 cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac
    16501 cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca
    16561 agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt
    16621 tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt
    16681 ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct
    16741 tacaacaaaa cgggctgaat tccctattga agaaggactt ttagctattg atgaaagctg
    16801 gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa
    16861 aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa
    16921 gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag
    16981 ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg
    17041 tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt
    17101 aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg
    17161 gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat
    17221 tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt
    17281 attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa
    17341 aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata
    17401 ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa
    17461 agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg
    17521 ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga
    17581 ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg
    17641 taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata caaatgattc
    17701 tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaaaa atacgtcgac
    17761 tcccttacaa gagagccgac gtattttttt cgtttctatt cgagaaaatc aaaaatagaa
    17821 agccttataa acgatcgatc aattcttcta attcatttag acgttgttca aacatcgaca
    17881 ttgcatcttc aatatatgca gcctgtgtca tatcaactcc tgcttttttc attacttcca
    17941 caggataatc gctgttacct gcttttaggt aagccaagta gttctctagt gcctctggtt
    18001 cctggtttag tattttcttc gccagtgctg aagcagcaga aaagccagtc gagtattgga
    18061 aaacatagta attataataa aaatgcggta tccgtgacca ttcgaattta atttctgggt
    18121 cttcttcgac tgctggacca taatattttg cattcaattt accataacta tcacttaggt
    18181 attcactagt caatggcaca cctttttcat cttccgtatg catgaaatgc tcgaattcag
    18241 cgaattgtgt ctggcggaaa actgttcctt taaacccatc caagtaatgg ttgagtacat
    18301 aagcccgtac acgagggtct ttttctgttt ccaataaata ctccgttagg atattttcat
    18361 tcgttgtcga agcaatttct gctaaaaaga tggagtagtc gccgtacaca taaggctggt
    18421 tcgaacgagt gaaataacta tgaacactat gtcccatttc gtggacaagc gtaaatagct
    18481 gatccagcgt atcatgccaa ttcaataaaa tatatggatt tgtgtcatag cttcccgaag
    18541 aataagcatc gcttcgtttc cctttatttt cgacaacatc gatccaacgt tcagagaatg
    18601 ctttttctac gatggccatg tattcttcac ccattggttt cagtgcttct aaagcttttt
    18661 cttttgcttc ttcgtacgta aaggtaattg gagcttcccc taagaccggt gtataaagat
    18721 catacatgtg cagtttttcc acttctaata aacgtttccg taattccatg tatcgatgta
    18781 acaaaggcaa atgcttgttt accacgtcta ccaaagtatc gtatacactt tcaggaatat
    18841 gattattgct caaagacgct tctctggcag agctgtaatt acggactttc gctttaaaat
    18901 tatgtccttt tatatgtgtg cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa
    18961 cactgtacaa tcccttaaat gctgcttcgc gcacccttcg atccgtgctt tctagcaact
    19021 gaccatacac gccatgagat aattggacta tttcaccatt ttctccttca atcgttggaa
    19081 aaactagatc tgcattattc aaaacagcaa atgtatcact tgaagcatca aagatttcac
    19141 ctgctccagc aagaagagat tcctgctcag cagataagac atgggctcga ttatctacta
    19201 tttgctggat ataatggcga tagacttcca attttggttc ttctttgaaa tattgccaaa
    19261 tctggtcatc tgacaattgc aatatttctg gttcaaacca ggaaacggct tcactaactt
    19321 tcgaaaacag actgcttgct cttgcataaa gcgcttggta atctgtattt ccagtgtctt
    19381 gatcgttttt aaggtgcgca tatacataaa tgacttcagt ttggcgataa accctcaata
    19441 cgaattcaat cgcatttaaa aattgagaag cgccttgatc aagtgttcct ttgtgttttt
    19501 cagattgttt taactcttct gataattcca aatatttctc atcaaactct tggtcgcttg
    19561 aaaagatctt ggtcaagtcc caagttaaat tttcaggcaa ttcttctcgt tttggcaact
    19621 gctttacttc catgaaatcc actccttttc ttcccttagc tctatctatc aaaatagatc
    19681 atagtctaac atccacatgt cacagcatct aacttttcta ctcattcatg tgtctgcttc
    19741 catcaatttt agcataacta aatcttgttt tacatagaaa accgattcct agcctatttt
    19801 tatacttact attttttcaa agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag
    19861 accatctctt catcaatcat aggaaaaagc cattcccttt tatgttcttt tatttggcaa
    19921 agccatgagt tgaaatcagg aactttctgg ttttccaagg cttcttcgta caacattcga
    19981 taaagaaaaa cctgttcttg taaataaaag aaaaaccgac ttttttgata gatccacgat
    20041 gatagatgca aaagatgttt atgacgcaag tagcattgtt cttgtatgga tactgtattc
    20101 ttttgcttgt gaataagttg gcgtgtcagc catttcttcc catcttcact cactttttct
    20161 atttttttca tttggttcat tttaatttcg ttactagtga atatttcttt aaggtcccta
    20221 ctagaaaaag tccagcagct actgcaaaag gaaatatgcc ctgatacagt ctctgtaata
    20281 tgatgataca aataaagctt ttgtttcttg agatcagctt tccagcaatg cactcctctt
    20341 tttctattat agtagcagca acttttttct atcaatgaga attgcgattg tccaagaaaa
    20401 gaattaccaa gtatccacca tacttggtaa ctgttattca aataattttg ggttctttca
    20461 ataaatctct ggtgagacag tctagaacat tggatttcta ttgctagatt cgccaacaaa
    20521 atatccggcc tttgccgcaa cctcggcaaa taggcttcca aaaatactgg ttctactgac
    20581 tgtcccagcc aatccatgaa gcattccttt aactgtaagt gctcttttga ttctctttca
    20641 ctaaagaccg aacagttatc tgatttttga tgggcaaaat gagcacattt tttctttccc
    20701 tttttcaaaa tcactctttt tcgacagcca cgacaataat atttctttcc tttttctgca
    20761 tgtttagcaa aacagcactt attatattca ttcattgcaa gcagcatttt tctccctctg
    20821 aacttattta cgtctaagaa tttatttaca cgaaaaaaga gggcgtgaaa aagctattct
    20881 gcatcaagta ataagaacag ctaaacaaaa atagttccca atatttttcg tttggttggg
    20941 cttgttaccg cagatgcaag cttttgaaca catttatacg aaaaaaagac tgcgacacaa
    21001 tttttgtcac agtctcttac tataatgctg atttatacaa aataaaattt cgttcgctcg
    21061 atagcattac gcgccatcag acattgtcca tattcattca atgtttctgg tgtacgatcg
    21121 ctcttttcag caaactccaa aattcgagca atctgattct ctacatccgc ttggttgaga
    21181 ttttccatcc agaagtgaac gactaggtaa tagcgctctt cgaataaata caaatcagtc
    21241 caagcagact ctagttcgac atctgttgca agttggatca cttgttcaaa atcttcaaaa
    21301 gcaaaagttg cttgtctttc ctgttcttct agggcgcttc cagataattc ttcctccgga
    21361 tcgctgccaa taatctgctg acgaagaaag tcactcacat cttctgaatc aacagtagaa
    21421 tcttctgatt gagaaatttc ttcatccgga agatttttac tgataaacag ttctaatccg
    21481 tctccttttg gcaaaacttg gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata
    21541 tcaacttctt ccaaaatact ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa
    21601 tccaaaaatg aaacgccccg ttcctctaaa tcttcatgac caattagtac acggatcgta
    21661 ttttcattaa tatgttccat ttccattttg ctacacctca ctttgccaca atgtttaata
    21721 tagattcatt gtagcgaaat cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga
    21781 atttaataaa accagcggat ttttcaacct ttcaagctat cttatttaac tataacattt
    21841 ttggcagacg aatactatat agccgacttt tcagctaatt ttccctctct atttttcgtg
    21901 caatcatcat cttctactac ctttttatac atctaatcaa gaatactttt tgtaaaaaca
    21961 aaaaaacttg gaaaaactat ctttgtaata gt
//
LOCUS       DAZUMT010000046        21779 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000046 DAZUMT010000000
VERSION     DAZUMT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.046"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..2801
                     /locus_tag="ACG8FM_001547"
     CDS             126..2801
                     /locus_tag="ACG8FM_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS5094212.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGIAKIEATFVEAQKKIWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2983..3222
                     /locus_tag="ACG8FM_001548"
     CDS             2983..3222
                     /locus_tag="ACG8FM_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094213.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            3238..4611
                     /locus_tag="ACG8FM_001549"
     CDS             3238..4611
                     /locus_tag="ACG8FM_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5094214.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            4615..6252
                     /locus_tag="ACG8FM_001550"
     CDS             4615..6252
                     /locus_tag="ACG8FM_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5094215.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6415..7347
                     /locus_tag="ACG8FM_001551"
     CDS             6415..7347
                     /locus_tag="ACG8FM_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094216.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            7463..7783
                     /locus_tag="ACG8FM_001552"
                     /pseudo
     CDS             7463..7783
                     /locus_tag="ACG8FM_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            7961..8320
                     /locus_tag="ACG8FM_001553"
                     /pseudo
     CDS             7961..8320
                     /locus_tag="ACG8FM_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            8384..11041
                     /locus_tag="ACG8FM_001554"
     CDS             8384..11041
                     /locus_tag="ACG8FM_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5094217.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            11113..11589
                     /locus_tag="ACG8FM_001555"
     CDS             11113..11589
                     /locus_tag="ACG8FM_001555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5094218.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            11603..12391
                     /locus_tag="ACG8FM_001556"
     CDS             11603..12391
                     /locus_tag="ACG8FM_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5094219.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            12393..13190
                     /locus_tag="ACG8FM_001557"
     CDS             12393..13190
                     /locus_tag="ACG8FM_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5094220.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            13209..13604
                     /locus_tag="ACG8FM_001558"
     CDS             13209..13604
                     /locus_tag="ACG8FM_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5094221.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            13606..14727
                     /locus_tag="ACG8FM_001559"
     CDS             13606..14727
                     /locus_tag="ACG8FM_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5094222.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            14747..15616
                     /gene="scrK"
                     /locus_tag="ACG8FM_001560"
     CDS             14747..15616
                     /gene="scrK"
                     /locus_tag="ACG8FM_001560"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5094223.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            15817..16326
                     /locus_tag="ACG8FM_001561"
     CDS             15817..16326
                     /locus_tag="ACG8FM_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS5094224.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16426..17076
                     /locus_tag="ACG8FM_001562"
     CDS             16426..17076
                     /locus_tag="ACG8FM_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094225.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17527..18465
                     /locus_tag="ACG8FM_001563"
     CDS             17527..18465
                     /locus_tag="ACG8FM_001563"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5094226.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18478..19530
                     /locus_tag="ACG8FM_001564"
     CDS             18478..19530
                     /locus_tag="ACG8FM_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5094227.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19796..20431
                     /locus_tag="ACG8FM_001565"
     CDS             19796..20431
                     /locus_tag="ACG8FM_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5094228.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            20622..21413
                     /gene="msrA"
                     /locus_tag="ACG8FM_001566"
     CDS             20622..21413
                     /gene="msrA"
                     /locus_tag="ACG8FM_001566"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5094229.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7400 a         3238 c         4438 g         6703 t
ORIGIN      
        1 ttttttcttt tgtcaagatt ttgagtggga tatatttttg tagaactgaa gatcatgaaa
       61 ttatgagagg ttggaggtaa tgtagtcata caaataaaaa ttttttagaa agtgagtggt
      121 aattaatgaa aatgaatcac acttcaattc agaagcattt acaactgtta aatacactgg
      181 ggatttctta tgttaatttg agaacttttt ctacattaaa caagaaaacc gtttctcata
      241 ttttttctac tgcagattct caagcaatta ttaactattt agaaaaacag aaattgtctg
      301 aaggtacagt aaacataact tataatccta ttaaacaaga ggtgattaca caaaaaaagc
      361 attctgtgag agacaatgat attgaaaaaa ttcggtttgt atttattgat attgacccta
      421 aaaggaaaga gggaagtgca acagatagcg aaaagaaaaa ggcagaaaat gttatggaac
      481 aagtagaacg ttatttgaaa gaaaagagaa tagaatcatt tgtaaaagtt gatagtggga
      541 atggctacca tatttttctt cccataaatg aacaaccaaa taatcacgag acgattctta
      601 ctattcagaa ttttcttcaa ttacttcacc gaagatttgg agtagaagat gaagttgata
      661 ttgatacttc tgtttataat ccttcaagat tatgcaaatt agttggtaca ccagcggtca
      721 aaggaaagtc cacaaaagag cgtccgcatc gtttatctag tttcctttct attcccgata
      781 acatagatag aagtaattgt tttaaaattc ttgaacagat agttgaagaa aattccatga
      841 tgtcaacctc tagagtaaaa agccatccat cttttagaga agtgttacct tttattcaag
      901 cagactataa acaatggtta gctcactatc ctgaattaac ttataccata aaagagggcg
      961 atcataaagg agttacctta cttatatttg acgaatgtcc attaagaaaa cacacgaata
     1021 attctaatgg ttcttgtttg tctgttacgg ctgataataa gattcgattt cattgtctgc
     1081 atgcttcgga taaaaataaa gatattcaag actttgtaaa aaaatatccg ttgccaaaac
     1141 ctgtaaatcc ttctgtagaa tcattagaat ccaatcaaga atattatttt gaccagttta
     1201 aactgaatca agaaggagta tttttggtga aagaaggtca agaagaaaaa gttgcttgtc
     1261 cgatgtttat taagcaaata cgcaaaaata tcgaacaaaa agtcattaca cttgttcttg
     1321 cctattatgt cgatcattgt tgggatgaaa tagaaataga aggtggatgt ttacaaacta
     1381 ataattttaa acaattagca aaacatggct taatcattaa agctagaaga gaagctgaag
     1441 tcgttgactt tttacagatt caacgggaga atgctccaat cttttttgaa catgaatctc
     1501 ttggctggaa aaaagaaaat gaagcaatga tttttcagtt acaagattct tttggacgtg
     1561 aaattccttc tgtattaact aaaaactcat gctaccaatt aacaactaat ggtacagaag
     1621 cggaatttga cgcattggtt cataagtatg ttttaggaac gaacttagaa cttgcttggt
     1681 cgattggtat tgcttctatt gtgttaggct atttaaacgt tacgaaggct aatacttttt
     1741 tgagtttact aattaaccta cagggcaaga gcacaacggg taaaaccact acgttacact
     1801 ttattgcctc tttatttgga aatccaacta cggttcttag aaatatgaat gctaccaata
     1861 aagccattat aaagctagca tcaaacaatc agggaggtgt tccactaata ctagatgagt
     1921 taggagcagc aactcaaaat aactttagct cgttgatcta tcaattagct tcaggagaag
     1981 aaaggctaag attagattct aattgtcagc ttatgccaca agagactttt tctgttttga
     2041 cccttacctc ttctgaagag cgtttggaaa attatttcaa tgagaaatta actggtttga
     2101 aagttcgata tatcgaattt tctgatatca agtttacaaa ggatgcgaat cacgcagaga
     2161 aagttaaaag tctttcttct aatacttttg gttggggtat aaagttattt cttttgaaac
     2221 tctttgaaca agggattgca aaaattgaag caacttttgt cgaagctcaa aaaaaaatat
     2281 gggctgtttt acctgaacat tctttgaagc aacgcattgg taataatcta gctttgattt
     2341 atagtggagc tctcctaatt caagatatac tgggctatcc tttacgatta gagcatatac
     2401 agacggtgct tattaattcg tacgaacagt taattgtaga tactagttta ggaagtgagc
     2461 aaacggtatt tgaccgagtg aaaacgttgt tggtgatgaa tagccataaa tttttaacaa
     2521 acagtgacaa caaacatacg cccagtacga tttggggaaa agtatcaatt caaaaaaatg
     2581 gggatattaa ggtaaatatt tttcctatcc cgtttgagga cttgttaagg cgagaatttc
     2641 aagtaaagga tctaacctat ctttttagac aattgcttga ttcgggtaat atgtgtgcag
     2701 aaaaagggag aagaacgaaa cgtattcaca tgaatcgtca agttcttcca acatacgaga
     2761 ttctattacc ttcttcactc aaatcgtatt ttcgattgta atttagaata aaccaaagta
     2821 tgaaagtgag gcaccatgtt atggaaatat ttcttattga tttagcaaaa caactgatta
     2881 cttatctgta tgatgacgag caaacccaaa cggaagtaaa tgaagcttga tctatgcttc
     2941 atttacttca ttcaatcaaa catttaagaa aggggaattc taatgactaa aaataaaatg
     3001 acactaaaag cagaggttct tctgtatatt caagaacatt tttctaatca agcctttttt
     3061 acaaaaccaa tttacttggc tttcgaaata agaggggtat ctgctggatc tattggaggt
     3121 actcttcaag cgttgaagaa cgaaggatat ttagaaaatc actttgtgca acgttcgttc
     3181 aatggaagag tcgcgaaaga atggtattta gttcattcat agagaggtga ttcaagcatg
     3241 cgtgtggctt gttatgatcg aacgtctgtt tatgaagaag cgaaaatcag taatgacgct
     3301 caatatgatg cagtgaaaga gtatgtttta gagaaatatg aacaaattcc tcaacgattt
     3361 tcagacaaag gagaatcagg tggaaaagcg gatcgtccag cttttcgaca gcttagagct
     3421 tgggtttgtg cgtcagaaga agagggggtg atctatgtct ggcgttatga tcgattggca
     3481 cgtcaagcca aaatagcctt agaatttatt gagttgtgcc aacaacatca ggttcaagtt
     3541 tattcgattt ctgaacctct tcctgcttct ttctcgcacg cgtcaacgaa taaactattt
     3601 gtgcaattat tgttcacttt tgcagaacaa caacgaaacg tcattattga aaatgttcgt
     3661 gccggacttg cctataaaaa gcaacaacgg caatatttgt cttctgcggt tccatatggg
     3721 tatcgtttag tgaatgggaa aatccaacaa gaagcacatg aagcaaaagt tgtccgccat
     3781 atctttcaac tttatttgac taaaaaatat ggctacaaaa agctttgtca acgattgacg
     3841 caacaaaagt tcttcttccg tgaacgtcct tttcagccct atcatattta tagtatccta
     3901 aaaaatccat tgtactatgg cgaaatcaaa ggaggatctt tggggaagta tctaggaacc
     3961 tttgaaccga ttctctcgaa aacaatcttt ttacaagccc aagagattcg ccaatcaagg
     4021 tgtacagcta aaaaagatac gtatccgtac ttattacgtc aaaaaattcg gtgtcccttc
     4081 tgtggtcgtc atctgagttc gaagtatcaa tggaatacaa agaagacgaa gacgcttcat
     4141 tattatcatt gtaccgaccg aagctgccaa ggaatctttc ttcgggcaga aagggttgag
     4201 tcagaagtat tggaaaagat acaggctttt tttcaatacg aaggcgtcta tcaagccttg
     4261 attgcccaat tgacgcaaca attagccgaa aagcgaaaag atgaaaaaca caaagtaatc
     4321 caacaaaaga aacaagaagc acaatggctc aaggcatttg aagagggcaa ttgttcactg
     4381 gaagaactca agcaacagct gcaagctctt caaaagccga ggtctacttc ttcgtttctt
     4441 tcactccaac agtgtgaagc aaaagtgaaa gaatggcttc aactaaaaga cctctctagt
     4501 cgacaattgt tatgtgacca tgtttgtcgg atcgaggtaa caaaggaaaa aacagtctgt
     4561 gggatttact tccgacaaat gacacaaaat attttagaag ggaacgaatg atctatggaa
     4621 acgacaccaa ccaaacgagt cgcattgtat attcgggtga gtacccaaga acaagcttta
     4681 gaaggacata gtattcaagc acaagaacaa aatggacgac agtttgccga acggatgagc
     4741 tatgaagtgg tagaagttta tgccgatgaa gggattagtg ggaaatccac aaaaaatcgg
     4801 ttcgcctatc aacgaatgat gaacgatgct cggaaccatc gttttgatct actagtgatt
     4861 tggaaattga cccgtctcgg acgcaatatg ttagatgtct tacaaaccgt cgaggaactg
     4921 ttgcgatatg atgtggggtt acattcgatc tcggaacaat ttgacgtgac tacttcgaca
     4981 ggaaaactaa tgttacaact ccttgggagt ttcggtgaat ttgaacgcaa ccaaattgcg
     5041 gagaatgttc agatgtcaat gcaaagctta gtgaaagata aaaagcgtta tgcaggtgga
     5101 cgacgtttag gctatgtcag tggtattgac acgaatggtc aaaaacaatt ggtcattgaa
     5161 ccagaagagg cgaaaattgt tcgtctgatc tatgcaaaat atttaggcgg agcagggtat
     5221 cgagcgattg ccaatttttt gaatcgtcaa ggatatcaaa cagtcaaaaa gaatgctttt
     5281 tctaccacgg cagtcaaaga tatcttacgc aataaaattt atgcagggta tttagaatat
     5341 gcacgctata tcaattggga aaccaaacga cgaaagggca agaacccaaa accgatttta
     5401 gtgaaaggta cccatgaacc gattatttcg gaagaagatt atcaaaaagt gcaagaacgc
     5461 cttgttttag aaggcaaaca gcccaaatgg aaccatacag gtgaaaatgt attgacaggc
     5521 ttgttacgtt gtccagaatg tggggcaccg atggcagcga gtaatgtcac aaatactcta
     5581 aaggatggca caaagaaacg aattcggtat tattcttgtt cagtctttcg gaataaagga
     5641 gcaagtgtgt gtcatgccaa ttcgattcgt gccgaacaag cggaaacatt tgtcgccgac
     5701 cgattaaagg agatcgttca gctaccagaa gttcttcccc gattggtggc agcgttaaat
     5761 gaagaaatag tgcgacaaag ccaaccattg gaacaagaac tcgtggtgtt actggaacga
     5821 aaggaagaat tgaagactaa aattgaaaaa tgggaagctg ctttggaaga tagtcctgaa
     5881 ttgtttccga tgttaaaaga ccgcttagac gaattgactg aaaaacgacg acagttgcac
     5941 attcgagaaa atgaaatctt agggatcttt caacaacaag gagagccgat tcaagtcaaa
     6001 gatgtccaac ggatattgac tagtttggat cgttttcttg cccagagtga gaagaagcaa
     6061 gtcaaagcct tgtatcgtac ctttattgag aaaataacct ttgatcctaa ctataaggag
     6121 atattggaga ttacaatgaa attcacgcct gccgtggtac aacaattgaa tgagcaatat
     6181 caaatagccg tatccaaaac aaaggatacg gctatttttg tgttaaagac accctttacg
     6241 ttaacgatct agtctaggtt atatcaaaca acgttagagg ggcgctaacg cttctctagc
     6301 gatttgaaat cgttagatga gtcgttaggg aggtccttat cataaatagg gatatttatt
     6361 aatgaatatt tgaaatataa ttgtatataa gaattaaaaa gagtggggat agatatgaaa
     6421 aagatgggta agtgtatttt atgtgaaaaa tacactgaac ttactaaaga acatgtacca
     6481 cctaaaaaat cagggaatac tggaggtaaa aagaccagaa ctggtaattt agatgatttc
     6541 cttaagagcg attttacaaa aggtgatttt ccgaagggga ttaaaaggaa acctcaagga
     6601 aatgtttatt atacgttatg tagtaaatgt aatagttttt ttggtagtga gtatgttgag
     6661 gaatatatta gatttgcaga agacaataaa aatttccttt ataacaatac aagtttaaag
     6721 aatggtaagt ctgatttaac acattcaata aagaaaatga attcacttag agttgcaaaa
     6781 gcaattgtag caatgttttt tagtttaaat ggggatgaag attcaatgga taagccattc
     6841 ttagattcgg tacgagaata tcttagcaat cctaaaagta ctttatttcc tcatgaagac
     6901 tacaaaatta taatgaatta ttatcttcca ataggtgctc cacagaattc taaaatcaaa
     6961 attcctaatg acattgtaaa tgatggtgac actcacaatg taataaaagg ttttatgccc
     7021 cttgatctat caactgggag aaaaattcga ttttcagaaa ttcagtatga aggaattggt
     7081 tttactctta tagatttaaa aaattcaact tttgaaataa aagaaggatt agatttaaaa
     7141 aatctccttt gtgcagatga taaaatcaaa aaaatacaat tgtacaaagt ccctatatta
     7201 acaccaacag tacttacaat gttggagatg gaaaataata ttaattatat tgactcaggt
     7261 tcagatttgc tgattaggtg gcaggtaata actaattttc ttatggaatt aaaaaaatac
     7321 agtaatactc aaacgaagtt ctcttgaagt tataaagaat acaataaata taaatttcct
     7381 ttagactata ttaaaatcag caatcgaagg aaaataaacg aagaaaatgg tacgcaaaat
     7441 ctaactatta gagcaaaaac aggaagctgc tttggaagat agtcccgaat tgtttccgat
     7501 gttaaaagat cgtttaaatg aattgactga aaaacgaaga cagttacaaa ttcgagagaa
     7561 tgaaatctta gggatctttc aacaacaagg ggaaccgatt caagtcaaag atgtccaaca
     7621 gatattgact agtttggatt gctttcttat ccagagtgag aagaagcaag ttaaagcttt
     7681 gtatcacacc tttattgaga aaatcacctt tgatcctaat cagaagtaga tattgccgat
     7741 cccgatgaga ttcacaccta ctgtgatttg ataattgaat gagtaatatc aaatagccgt
     7801 atccaaacca aaggatatgg ctatttttgt gttagggata tacgtcacgt agcttgttat
     7861 caagaataag tttctttatt tttttgcttt ttctaaaact gagtatgcta aaataagagg
     7921 gtatatagca actttaaata agagatagga gtgtatcttt ttgggtgcag aattaaatca
     7981 gaagttattt agtgcggcag ataatctccg tagtaaaatg gatgcgtcag aatataaaaa
     8041 ttatttattg gggctgattt tctataaata tttatccgac cgtttgttgg aacaagtggt
     8101 tcttttagca gatgaatcct tagaggaata tgacacagta agcaaacaaa cgatgttgta
     8161 tcgtgaatta ctttcagatg aagaatcaaa agaagatctg attgcaacaa tcgttgatat
     8221 tttggggtat gcgatttcgc cagagtatct tttcaacgtc ttagcggatc aagcaaaaca
     8281 agcaacgttc caattgaatg atctaaacaa agccttttta gcaagtcata tttatggaaa
     8341 aatactgtcg gcaaaattcg aatagtgtcg aggaggtgct gtgatggaag aattaaaaga
     8401 acgcattgtc aattatgtca tagagaactc tgagattaaa aataaatggg ggaacaatac
     8461 agcacagaaa atatctgaaa aattaaatgt tagtcgtaat attgtctctc aatatttgaa
     8521 tgaattcgta aaagatggag aatttggaaa aatcaatact cgtccagtaa tcttcttttc
     8581 tttaccggag aaagttccaa atcaacaaat ttttagctct tcagaggaag cggaaaaatg
     8641 gtatgatagc tcttttaggg ttggaataga aagggttatc ggaagtcaag gaagtctgcg
     8701 aaatgtaatt gctcaaatta aagcagcaat ctactatccg ccaaatggct tacccatttt
     8761 acttcatggc gagactggaa ctggaaaaag ctatttagca aattatgtgt ataaggattt
     8821 gttggccaat aattatatag aagagaaggc atctttcatt tcagttaatt gtagtgaata
     8881 tgccaataat ccagaacttt tcttgtccaa tttttttggg catgtaaaag gtgcatatac
     8941 aggagccgaa gatggaagaa agggtttagc agaattagct aataatggag tcttattttt
     9001 agatgaaatt catgcattga gcaatgaatg tcaggagaaa ctttttcagt ttatggatac
     9061 gaggaaattt catcggatgg gtgataatga aaactggtac acatcaagtt gctggattat
     9121 atttgcaaca tcagaaccac ctgaggataa actattatca actttgttga gaagaatacc
     9181 tgtaaagcta acacttccaa cattagacag tcgttctctt tatgaaagaa aacagttaat
     9241 caagatattt tttaaatcag aagaagaaag actagcaaag aaaatactga tatctcaaca
     9301 attattagat tttttattaa actatcgctt tagtgaaaat gttggcaatt tgaaaaatat
     9361 cattcaattg acctgtgcca atgcctttta tttcttagat gatttggaag aactttatat
     9421 tcagatgtat catttaccac aatgggtgaa tttcaacggc atgaaggtta tgaaaagaca
     9481 gaatgaatta aaatatattc aaatggatga tttggaaaaa gtagacgaag tttttaatat
     9541 caacgaactt atagagtatc tttatcaaac gtggaagaaa gacactgata agcagcagct
     9601 ttttgagaaa tatgatagat attttagaca aaagaatgtt gatatccaaa ataattcagc
     9661 tttaacatat cccacctatc aagagattcg atttagacag tatgttaagg aaatagagag
     9721 agattttcaa atagaatttg actattatta tcttcaaata acgtctatct ttttgaattc
     9781 ttggatttat caaatccaat taacatcgga cgataaatta tattccttca tcaatgatat
     9841 taatagttgg tgtagtcgag aagttcaatt agtcaatcgt tttattcaaa taggggatat
     9901 ttatttaaca aaaattcaaa aaaatgtttt ggttatttta tttaagttaa tgtgtagtaa
     9961 agaatttcag caaaaacgtt tagcactgat tttggctcat ggagatctaa cggcaacctg
    10021 tatagcaagt actgctaata ggatcgttga gagatatata tacgacgcca ttgatatgcc
    10081 actaatgact tcaagtagtg agattgcaga caaaattaat agatacttac aagagtgtac
    10141 cggatatgaa gaaattattt tacttgttga tatgggttca ctggagaaaa tttatctaaa
    10201 tttggaacta gacgctcaaa aaaaattcgg actactaaac aatgtttcaa caagaatggc
    10261 tattgatgtt gcaaatcaga tattattaga tttaccgctt aaacagattc ttgaacaagt
    10321 aaaagaaaat caaaatgtgg attatattta tgaatcatca aatattaaga aggatatgat
    10381 tgtctgttct tgtgcaagcg gattaggaac ttcgtttaaa ttaaaaaaga tacttgaaga
    10441 tagctttcca agggatactg atcttgtcat agaaacgtgt gactattact ctttagtgaa
    10501 tgaaaactat ttatcggagc ttcgtaaaga gaacaatatt ctttttattg ttggaacact
    10561 aaatccacat ctagaagatg tagaattctt ttcaattgaa gatatgattt taggaatgaa
    10621 tataagtaaa ttctccaatc aattgaatag acactttaat gataatgaac tgaaagtttt
    10681 gaacaataat attctgaaga attttagtct aacgaattta atggagcaat tgaccatatt
    10741 aaatccgact aaattattag aacgggtttc agatgctata tacatcttac aaaatgatct
    10801 aggaatttca tttgataata atacgtgttt cggattatat gttcacatta gttgcttaat
    10861 tgaacgatta gtaaaacaaa atactttgga agatgaaatt tatttcaatg aaacgagtga
    10921 ggaatttcaa aaatttcaga cacattttaa gcaatctttc agtgtcgttg aacattatta
    10981 tagtgtcgac atcccgattc atgaggttaa atacgtttat gattatgtaa aaagagcttg
    11041 atttatcaag ttctttttgt tttggcatgg taattgcttg taagaaaatg aaagataaag
    11101 gaggaagtat ttatgagtat tgttatgtcg agagttgata accgtttatt gcatgggatt
    11161 attgtcacgc aatgggcacc aatgtctaaa gcaaacagag tgatggtgat cgatgattct
    11221 gtcgcaaata acgacatcca aaaatcaagt atgcgtttag ctagacctgc tggaatggct
    11281 gtatcaatta tcactgaaga aacagcgatt gcgaatttta aaaaggggaa atacgatcag
    11341 gaaaaagtat ttgtcatcgt gaaagagccg gaaacaatcc ttcatttagt agataacggt
    11401 attagaattg accgactaat tgttggcggt actgttgtta aagataatgc attacagcta
    11461 tcttctagag cctatgcatc agatgaaaat atcagcacgt ataaaaaatt gttggattct
    11521 ggtgtagaaa ttgttacaca atttgttccg gcggataagg cacaggaaat tcaatcaaaa
    11581 ttaggataaa ggagtaagga taatgcattt tactattttt caaattatta tgctgactat
    11641 attagcattt attaaacatg ttgattatta tgggattccg atgatctttg taaattacgc
    11701 agttttttgg ggattattca caggaatttt gatgggtgat ataaaaacag gtttgatagt
    11761 tggcggtaca gttcaattga tgtcattagg agttgccggt tttggtggct catctgtacc
    11821 ggactatgga acgtgcgcaa ttattgcaac ggcatttggt agccagcttg gagtagaagc
    11881 cggattagca ataggactac ctgtaggtat gttaggggtg caactagatg taattgtcaa
    11941 aatcttaaat ggttttgttg ttcgcaaatc acaggaatat gcgaataaac gagaatttaa
    12001 gaaaatgaat agtgttttgt ggttggggcc aattttcttt ggtttatctg cagcaattcc
    12061 agtatttatt gccgttactt taggacagtc tgctgttaat tacgttttgg atgttttacc
    12121 taattggttt atggatgggc taacattagc cggaaaaatg ttgcctgctg ttggtattgc
    12181 gatgttgtta cgctttatgc ctacaggtcg atacattagt tatttacttg ttggattttt
    12241 cttagccgct tttttgaagg tacctattat tggagcggcg atcataggat tcgctttagc
    12301 atttaatgtg tatcatagag ctgaaaaaga tgcacaaatc actgcttctg cgggagcttc
    12361 tgatgttggg tttattgggg aggatgaata aaatgagcga aaaatatcaa gtaacgaaaa
    12421 aggatcttag gaaagcgaac tatcgttggt tgatgtcagt ttgtaccttt aattatcaaa
    12481 cacagcaagg tgcatctgtg acttacgcat tgtcacctat tttaagaaaa ttatacaaag
    12541 atgatgatga atacatcgaa gctttaaata atcactttca gtattacaat acacagcctt
    12601 ggctggcagc cattatttta ggtgcttgtg ttgcaatgga ggaaaagcaa ggattagaag
    12661 caaaagatgc gattaatgac tttaaagttg gtacgatggg tccaattgct ggtattgggg
    12721 actctctttt gatgacgatg atcccaacca tcatgggatc aattgcggct tatatggctt
    12781 tagaaaataa tcctgtagga atctttttat ggttcatttt aatcatggta atcttctttt
    12841 tccgaatgag aggctttgaa tttggttata agcagggaat gaagattgtt actgaatacg
    12901 gtgaaaagat taattattta actgaagctg cttcagtatt ggggctaaca gtagttggat
    12961 cattaattgc ttcagttatc agtgtttcaa cgccacttaa attcactttt ggtgaagtta
    13021 gtatgaaaat ccaaccaatg ttggacaaaa ttttaccaac attaattcca gtaattatta
    13081 ctgctgcagc atattatcta ctcagtaaga aaaaaatcag tatgaccatt ttgattctac
    13141 tcgttattgt ttttgcgatg gttatggctg catttggaat attagcgtaa aggaaaagga
    13201 tttgaattat gagaagattg atcattgcta gccattcaat actagctgag ggttttaaag
    13261 agacactaga gtttttaaca ggtatgcata atcaaattag tagcttatgt gcatatgtcg
    13321 aagaaattga tgatatagat gaacaaatta aagagttagt ggatactaca gacgaaataa
    13381 ttgttttaac tgacatggct ggaggaagtg taaatcaaaa gttttttacc tatatttctg
    13441 acagagtgca cgtaattgca ggaatgaatc ttcccttggc aatgtcaatc gctttgcagt
    13501 taggaaatga taatttagat attggagcaa ttattgcgga ggcacaacaa caaatcatct
    13561 atatgaataa tcaaaaaagt gaagaaagga tagaggacga ataaaatgaa ttgtttaaat
    13621 aatatatggc tccacaaggg agaggaaagt agaactattt caccagaaaa taaaaatggt
    13681 gaaaaaggga aagcggcaat gacagcgagt catctaggaa ttgggagaaa agggtcacct
    13741 tctgttccga taaatgctgg agagacaatt actttagcag aaatggaagg ccctggaatt
    13801 ttacgtcatt tttggatgac agtgacagac aaaacaatcg ctgggagttt cgtcttacgt
    13861 gatttaattt tgcgtattta ttgggatgat gaatccactc cttctgtaga aagtccatta
    13921 ggagattttt tttgtaatgg gtttgggaca agatgcgaag tgaattcctt accaatcgta
    13981 gtgaatccaa catcagggat gaattgctat tttgagatgc catttcgaaa aaaagctaag
    14041 attactttaa cgaatgaaca tcctgagttt attaagcaca ttttttatac atttaattat
    14101 gcattgactg aagtaccgga tgatgcgatg tattttcatg ctaaatggaa ccgtgaaccc
    14161 atcacgacaa aaggaaaaga ttacaccctt attgatggaa tcaaaggcac tgggtattat
    14221 gtaggaacct actttgcttt gtgcgcattg gaaagatact ggtgggggga aggagaattt
    14281 aagttttata tagatggaga tgaagatttc cctacaataa catctacagg agcagaagat
    14341 tattttggcg gcgcttgggc tttccataaa aagaactttg gagaaagggc atatgctcaa
    14401 aattttaata cgctatttat gggctatcct tttcaatcta aaagagatca cacccgggat
    14461 tattttagtg ctggaaaacc taatccaaat ccagttcatg gttttggcga tgatgcttta
    14521 ccgatgcatg ggttgtaccg ctggcattta cccgacccca ttagtttcaa agaaaattta
    14581 cgtgttactt tccaaaccct aggaaatgat gacatcaagc tttatgagcg tcaagacgat
    14641 atttcttctg ttgcctattg gtaccaaaag gaaccacatg agtttttcga aaaactatta
    14701 gacagggaag aacgaattcc tagataaaag gacggtggag taagaaatgt atggctcaat
    14761 agaagcaggt gggactaaat ttgtttgtgc cattggcaat gatgatttaa aggttttaga
    14821 aagagtgagt tttcctacaa ctacaccgaa tgaaacaatg agtttggtaa ttgatttctt
    14881 taatcactat aaagaacaat tagaaagcat tggagtaggg tcatttggac cgattgatat
    14941 ccatcgtgaa tcaaagacct atggacatat cacctctact ccaaaaactg cctggaaaaa
    15001 ttttgatttt gttggaacgt tgaataagca tttcgaaatt ccaattgcct ggacaaccga
    15061 tgtgaatgct gcttgttacg gggagtatgt ttcaggtcat ggaaaaggtt tatccagtgt
    15121 tgtttattac actgttggaa ctggaattgg tggaggagct atacaagatg ggatttttgt
    15181 tgaagggttt agccatccag aaatggggca tacgttagtt aagagacatt caggagatac
    15241 tttctctggt acttgtcctt ttcatcatga ttgtctcgaa gggttggcat ctggacccgc
    15301 tatcgagatg cgtacgggaa ccaagggaca agatctttct atagaagatc cgttctggga
    15361 aatcgaggcg gattatattg cacaatgtgc ctgtaatacg accctgatgc tttcaccaga
    15421 tattataatt tttggtggtg gtgtcatgca acaagaacat ctgaaaaaga aagtacaaag
    15481 acgtttttta gaactaatta acggctatgt cgatacgcca aatatcgaag aatacattgt
    15541 cacacctaag ttagcagata atgcaggaac tataggatgc ttaactttag caaaagatgt
    15601 tagaatcaat tcttaagcaa aaaagtatta gtcaataaaa tgcttatgaa gtccgatcaa
    15661 cttaatgtgg tcggactatt tgtattcagc aatatcattc actcatgata cccagcgcct
    15721 tatcataagt gatgcggttt ttatcattga tcctaaaaat cttagcaaga caaaaagtac
    15781 gtaagaaagt aaagactaaa tacttcggag gaacaaatga ataacaatga taggttattg
    15841 cgattgcgtt atgcgattga tttgaaagat acggatttga ttaaagcttt tgagctaggc
    15901 ggtgtaccac tgactaaaga agaagctcag gcggtactga ccaaagtaca ggataagaac
    15961 aaagataatg cagaaaataa tgtgtatgaa aaaaccatca acaaccaagt ttttgacgca
    16021 ttcctaaatg gcctgattct cttggcgcgt ggtccgcaaa aagataagcc gatagtagac
    16081 acgccgcaaa aaagcaagga aatcaaatat atcaacaatg tgttgctaaa aaaattaaaa
    16141 atcgccttgt ctatgaccac tgatgatatt ttagacgttt ttgctgaggc agaaatctat
    16201 ccatcaaaag gcgaaatcgg ggcatttttg agaaaagaag gccaacgaaa ttttaagcct
    16261 tgcggagata aatatatgcg taattttctc aaggggttag gaatttataa tcggagaaaa
    16321 gtataaagaa atttcatgag aaatcaaaac aatggttgtt ttgatgagag ctagtttttg
    16381 tatgagatta aaatattatc ttaatcgagg agctggattg gttttatgaa tgatgaatat
    16441 aagaatgatg aagataagat gctttttgaa gagatagaga atcgatgtag gctcaatttt
    16501 gagcttcggg gaaagatgtc tttaatacaa caaaaaaagt atttagcgaa taaatcagag
    16561 tttactctgg gacatgttga aaaattaatc tcggattgga ttagtagtag aagcgaattt
    16621 acaaaaataa aacagccgat taaatttgac atgaaaaaac ttttgttaaa taagagtgag
    16681 ataggtaata gagatcaata tattagagct aaagggcaag aaataattga ttcattaggt
    16741 gaaatgaggt catacaatta tttgtatgta acacatagag ctgatggaat ggtcattact
    16801 gtggggaaat cttcttctaa tgatattttt ttagatggag atctttttta tcaactcaat
    16861 acaaatcact tgtctggcac tgaaaatata attttaagga cggaatatgg caatgaaata
    16921 tttgcaaaat atgatgagat tttaaaaaat tatttggact gggcttggat aattccggtt
    16981 gagtcaggag atgctaagaa actggagaga ttattgggcg atgaactaat taataaaaaa
    17041 gtaccaattt taaattatta ttcccataga caataaaagg ctatttttca tgataatact
    17101 agttataact gtaaaaatgg aagaaaatct gggaaagaag tttttaaata agtgtattgt
    17161 tcgattttaa taaataataa gcaataccat ccgtctgtgt tgcgcaacat cgtactacaa
    17221 tgaatatttt ttcacaaaaa gaaaatcatc catcgaatga atggaaggtt ttagtataca
    17281 cgaggttaat gccccctctt ttataatcag aggagtgagg ttttttctat gaatgtttct
    17341 attttcttat tgctagtcta gtttctctta cgatgaaatt gggattctat tccagttttt
    17401 attgatttgc tgataaaata acttgaatgg tggatataaa catgataaat aagagaagaa
    17461 taacaaaaca aggttgtgaa atgattctca aatgaatata atagaaagaa aaaggaggaa
    17521 gaatcaatga aaatagcaat tgcaggcgct ggcgcaatgg gaagtcgctt tgggttaatg
    17581 cttcaccaaa gtgggaacga agtattatta atcgatggct gggcagaaca tgtccaacaa
    17641 atcaaagagc atggattaca agcaaatttt aatggtaaag aggtagaagc aaaactacca
    17701 atcgttcttc aatccgaagt agaaaaagaa gatcaagttg atctgattat tctatttacc
    17761 aaagcgatgc agctggaaaa aatgctgcag gatatccaat cattaatcaa aaaagataca
    17821 gaggtcttat gtctattaaa tggtatcggg catgaagata ttattgagaa atttgtacca
    17881 atggaaaata tctatattgg aaataccatg tggacggcag gtcttgaagg tcctggtcag
    17941 gtcaaattat ttggaagtgg ttcagtagaa ttacaaaatt taggtgatgg aaaagaagca
    18001 gctgcaaaaa aattagcaga caaattgtct gaatcaggat taaatgctca tttttctgac
    18061 aatattcact attctattta tcgcaaagca tgtgtgaacg gaacaatgaa cggactatgc
    18121 acaatcttag acgttaatat ggctgagcta ggaaaaacat cgactgctca taaaatggtg
    18181 gcgacgattg tcaatgagtt tgccaaagta gcagcagtag agaagattga actagatgtc
    18241 ccagaagtca ttgcacattg tgaatcttgt tttgacccgg aaacaattgg gttacattat
    18301 ccttcaatgt atcaagactt gattaagaac catcgattaa cagaaatcga ttatatcaat
    18361 ggcgcaattt ctagaaaagg gaagaaatat ggtgttgcga cgccttattg tgatttcttg
    18421 actgagcttg tccatgcaaa agaagatagt ttgaacgtaa aataaaggag agattgaatg
    18481 gaaacttcac aaaaaatatc accgaaaatt tttctgaata aagtcctcgc tggtacagca
    18541 acaggaatca ttattggatt gattcctaat gcagtattag gtgccatttt aaaatatttt
    18601 agtgcgtatc cctttgcggt aacgatcgca cagttagcag ttatttttca actagcaaca
    18661 cctttgatca ttggtggttt gattgcctta caatttggat ttgatccgat gagaatgatg
    18721 gttgtagccg gagcttcttt tgttggttct ggtgtagtca aattcacacc tgatttgggt
    18781 gaaacgggtg tttacgttgg tgctggtaca ggcgatatta ttaatacgat gctgacagca
    18841 tcgattgctg tcgggctgat tttactgatt ggaaatagtt ttgggtcagt gtcgattgtg
    18901 ttaacaccga ttgttgtagg tgtaggtgca ggtttacttg gtttttattt attccctttt
    18961 gtaacagcaa ttacttctgc aattggatca ttgattaata acttcactac cttacaaccg
    19021 ctgttaatgt ctatcttgat tggttgttcc tttgcctttt tgattatctc tcctatttca
    19081 acagtagcaa tcggaatggc aatccaacta gatggtgttt ctgctggtgc ggctgcgatg
    19141 ggcgtagctg caaccacttt tgttttagta gtgaactctt ggaaggttaa taagtcaggt
    19201 gtgacaattg cgattgcttt aggcgctatg aagatgatga tgcctaatct atttagaaaa
    19261 cctgtgattc ttattccttg tttagttagt gcagtgatta cagctattcc tgtcgctcta
    19321 ttttctattt caggtacgcc tgcttctgct ggtttcggcg tggtcggact tgttggtccg
    19381 ttggcttctt tagatgcagg gctaaatggc tttcttgttg ttctttgttg gttagtcatc
    19441 cctgtttttg cggcactaat tactcaattt ttatgcgaga aagtatttaa actgtatgat
    19501 cgagaagaag tcttcaaatt tttaggctag tagattttac taagaaattt tcaatcaatg
    19561 gatgcgtaag tgagatgtca acaacatctg acttacgttt ttttgccttt ttgagtagga
    19621 gattgatgct gacaactgaa ctcttcagtg ccattcgatt ttttaaaatg tagaagaaac
    19681 tcacctttat gttattgatt gttcaaaagg caactagaat aaaaatatgt gttaaaatga
    19741 gaaaaaattt gattgttgga tgtatcgaat aatcactaaa aagtaggaga agaaaatgat
    19801 ttttttgcaa aaatatggtt tttatttttt attattaggt gtgattagtg atttatcgac
    19861 accgtatatc cttggccttt tttatcccaa actgaatcaa atgactaccg taattagtgt
    19921 gtttggtgac gtagatagtc cagtcagacg tgctttcttg gtctggtcag ttgtatctgg
    19981 actttttttt gttctttcgt tgcctgcgtt gtatcaccta tttattggaa catcaaaaac
    20041 acttgcaata cttgctgttg caacagttgg tttatatggg ataggtgatt gtatttttac
    20101 tgggttattc agtatcaata cgaatgaaag ctcttggaat ctatcgactt ggatccataa
    20161 tataggttct gggctaggct atgcaggctt tctacttttc ccattgttac ttgttctgct
    20221 ttatcgtcaa agtggtcaag gtacgcttag ccatttttat cttgttttaa cagttattag
    20281 tttactaatt gcaggtcttt acggattagc acggattccg tcgattagtc agtttgcctt
    20341 ctttaagcaa ttgggcttct ttcaacgctt aagttttttc tttaactatt tgccgatgat
    20401 ttgtttaagt ttttttcaac tacgaaagta gaaaaagcag tgtgaagctg attaagtaaa
    20461 agaatttctc tatgggtaac cattcgtgtg gatgtgttcg actaagaata gagatgagga
    20521 gagcgaaaag caagttagtc ttaacttgtt atgcgttgtg ctaaatttca ggtagtcatt
    20581 ttttgaatga ataagaagaa aacttgaaag agggtgactt catgttagat gtggagcaga
    20641 tggaaataga cctttataat attattttaa atcgaaatac aagagaatgg gaaagaacac
    20701 aatttgaaga ggcaaaagaa gacctagcaa aaatgtcgat aaagcaggtg atagaaaaac
    20761 tagaggtcaa cttgcgtccg ctagcactac gagataattt gacaccagat gcgatggatt
    20821 tttatttaag aatcacaggg gagtcagcga gtgcaaaggc ttatgattat gccaaacata
    20881 agataacttc acccttatac caagaacggg cgatttttgc cggtggctgt ttctggtgta
    20941 tggttgagcc ttttgaaaca aaaccaggtg taatctcagt gttatctggt tatacaggtg
    21001 gagagaccaa aaaaccaaat tacgatcaag tcagtggtgg atatactggg catgttgaag
    21061 ctgttgagat catatttgat acacgtcaaa taacctatga agagctgttg gagatttatt
    21121 ggcaaatttc agaccctaca gatgcctttg gtcaatttca agatcggggg aaacaatatc
    21181 gttcaattct atttgttgtt aatgaaaagc aaaggcaatt ggctgaaaaa tcaagagaac
    21241 aactgattca atctaaccga tttagccgtc ctatcgttac agagattcgc caagcaaaga
    21301 ctttttggcc agctgaaaat catcatcaac aattttatca gaagcaacct aaaagatata
    21361 aaaagatcaa gcattcgcat cagcttcatt tattttttca gcgacataga tagaggaagg
    21421 ttgtggcgca aagcgttcag ctccgacaaa ataatgagca actgacaaaa atagcttttc
    21481 atatttttgg acagttatga attatttcga ggagttgctt tgagaacacc gaggaaaagg
    21541 ttgtggcgca aagcattcag cttcaagtcg caaggaaaca ggaggtatga caagtctttt
    21601 gtcacaactc ctgttttttt actagccttt atttccatgt gaaagagaat tcgaattgta
    21661 gaatcttgat ttttctatgg aattttgtag agaatcatct tgataattaa tagacaccta
    21721 atcgaattcc acattaatga tgaactaaat tccgtataaa agcgcaaaca ataactata
//
LOCUS       DAZUMT010000047        21776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000047 DAZUMT010000000
VERSION     DAZUMT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.047"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1422
                     /locus_tag="ACG8FM_001567"
     CDS             82..1422
                     /locus_tag="ACG8FM_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5094230.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1640..2083
                     /locus_tag="ACG8FM_001568"
     CDS             1640..2083
                     /locus_tag="ACG8FM_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS5094231.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2587..4005)
                     /gene="nhaC"
                     /locus_tag="ACG8FM_001569"
     CDS             complement(2587..4005)
                     /gene="nhaC"
                     /locus_tag="ACG8FM_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5094232.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4408..5631)
                     /locus_tag="ACG8FM_001570"
     CDS             complement(4408..5631)
                     /locus_tag="ACG8FM_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5094233.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5662..6501)
                     /locus_tag="ACG8FM_001571"
     CDS             complement(5662..6501)
                     /locus_tag="ACG8FM_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5094234.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6518..7198)
                     /locus_tag="ACG8FM_001572"
     CDS             complement(6518..7198)
                     /locus_tag="ACG8FM_001572"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5094235.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7200..8273)
                     /locus_tag="ACG8FM_001573"
     CDS             complement(7200..8273)
                     /locus_tag="ACG8FM_001573"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5094236.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8329..8567)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8610..8843)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8920..9591)
                     /locus_tag="ACG8FM_001574"
     CDS             complement(8920..9591)
                     /locus_tag="ACG8FM_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5094237.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9678..12326)
                     /gene="mgtA"
                     /locus_tag="ACG8FM_001575"
     CDS             complement(9678..12326)
                     /gene="mgtA"
                     /locus_tag="ACG8FM_001575"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS5094238.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12668..12842)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13015..13452
                     /gene="ndk"
                     /locus_tag="ACG8FM_001576"
     CDS             13015..13452
                     /gene="ndk"
                     /locus_tag="ACG8FM_001576"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS5094239.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13474..14340)
                     /locus_tag="ACG8FM_001577"
     CDS             complement(13474..14340)
                     /locus_tag="ACG8FM_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5094240.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14500..16593)
                     /locus_tag="ACG8FM_001578"
     CDS             complement(14500..16593)
                     /locus_tag="ACG8FM_001578"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5094241.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17342..17965)
                     /locus_tag="ACG8FM_001579"
     CDS             complement(17342..17965)
                     /locus_tag="ACG8FM_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094242.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18124..19521)
                     /locus_tag="ACG8FM_001580"
     CDS             complement(18124..19521)
                     /locus_tag="ACG8FM_001580"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5094243.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19553..20524)
                     /locus_tag="ACG8FM_001581"
     CDS             complement(19553..20524)
                     /locus_tag="ACG8FM_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5094244.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20898..21323
                     /locus_tag="ACG8FM_001582"
     CDS             20898..21323
                     /locus_tag="ACG8FM_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094245.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         6278 a         4499 c         3775 g         7224 t
ORIGIN      
        1 tttatcatta gtcattgtta aatattgcta tttattctta atataaattt tgattgtgaa
       61 tttaaaagaa agaggtcatt tatggaagat aaaaaattga aaaaacaact tagctccaga
      121 catatcacca tgttagcttt aggtggagca atcggtgcag gtctatttaa aggaagtggc
      181 gaagcgattg ggatcgccgg cccttctgta ttgattgctt ttttgattgg tgggctgatt
      241 ttatatatcg tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga cacgcatcac
      301 ggattatctg gtttgatacg cccttatcta ggtgctcatt ctgctgattt tactgattgg
      361 gtctactggt ctatgtggat tatcaatatc atcgctgaag ctgttgcagc tgcttccttt
      421 ttgcagttgt ggttcccgca cattccagcc tgggtatttg tttttatgtt ggctgtttta
      481 actacgatca tcaatcttta ttcggtacgg ttattcgcag aaacagaata ttggctggct
      541 tttgctaaaa tcagtgtcat tatcttactg atcatttttg ctacttatct tgtcggtcag
      601 caaatgttgg gcacaggcgt tttccctacc cttcagcaat tgactgacca tggtgggttt
      661 actcctcatg gtatgaaggg aatcatcagt tctttgcttg tagttatcta ttcttacggc
      721 ggttctgaac tgattgctat cacggtaagc gaagcagatg atcctaaacg tgcgattcct
      781 aaagcaatca aaggtgtcat gggaagaatc atttcctttt acatcattcc tttgtttttg
      841 cttttgattc tttttccttg gaatacttta gcaggcacga atgtcagtcc ttttgtgatg
      901 gtatttgaaa aaatgaatat cccatttgca gcggatatcg tcaattttgt gattgtcctt
      961 gctttgttct catctatcaa ttcgggtgtg tatgcatctt caagaatttt gttcttccgt
     1021 ttgaaggatc gaaaaggttc ctctagaaaa ctagctgttt taaacaagca tcaagtaccg
     1081 caacgtgctg ttttattttg taccagtgtt ctttatcttg gtgtagctct ttcttatttt
     1141 gtcggtgaca aattatttgg ctatctggca ggttcccttt cgtatacagt attattgatc
     1201 tggatcatta tcagtgcggc atcctttgta ctggcactaa gaaaaggaac attatgggat
     1261 aaaggaatca gcttttttgc attagctgtc ttaggattga ttttccttgg tatcctgttc
     1321 acgaactcca ttggggtaac tgtattgact ggcctgcttt acctgttcat ttatttcagt
     1381 tatcaaaaaa agaatgatgc gttcgtattg acgaatgaat gaaatcgata gaaaatttta
     1441 ttttatttat gtggaataga tggagctatt gaagaaagga gttgtgacaa aagtcttgtt
     1501 gcaactcctt tttccgaata aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc
     1561 ctctgtgcaa aataattgat tgttctccat gctcatgaca tgatagaaga aaggaatgaa
     1621 aaaaaggagt ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc
     1681 aacggtagtt atgtttggta tgatgtactt gaatgtctat gcgctcgatc atatcttctt
     1741 cagtcgtaca cgagtattca tggcgttgat gatgggtgca gtcatggcag tcatcatgct
     1801 gttgttcatg tggaaaatgt atgaaaacaa aaagataaat gttatgatct tagccgctag
     1861 tcttcttgtt tttgccggtt ccttgtttat ggtacgaagc caaactactg tcaacgacac
     1921 agcttggatg aaggccatga ttcctcatca ttctatcgct atcctggcaa gtaaacgagc
     1981 tgatttaaaa gatcctgaag taaaaaaatt agcggaagaa atcattcagg cacaagaaga
     2041 agagatcgac aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat ccgtccaaat
     2101 aaaaaaagag gatgttccag aagttatcag cttcaagaaa taagaagaag ctacttctgg
     2161 aacgccgtga ataaggggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     2221 acaaaaatag cttctcctat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt
     2281 tgaacaccgt ttattaagag gttgtaaaat cagcgttctg acctgagtat tagagagaca
     2341 aatggaaaaa tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga
     2401 ttgaacaccg tttatcgaga ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa
     2461 ttttgtgaaa tggctttcca catttttgta taaatttggc ttaataccga gaaactggct
     2521 tttgaatacc gtttattcgt aaaagggatt gtgacaaaag tcttgtcaca atcccttttt
     2581 atgtttttat tgttctaatt gcgctgtctt gcttactgct tcttgcggtt tcacaaattt
     2641 ttttgagatt ccccaacctg tgaaggcact gaccatcgaa aacacaggag aaagcaaact
     2701 gaaaaatacg aatggcaagt aagtgagtgt aggaatacca aatgtacttg ctacgaaact
     2761 tccggcaatt ccccaaggca ctaagtagtt gattactgtt ccaccatctt ctagcgttct
     2821 acctaataca gttggatcaa atcctgcttt ttcataagtt tctttaaatg cattaccagg
     2881 cagaataaca gataggaatt gttcaccaat aaaaatattt actccgatac ttgtcatcaa
     2941 tgttgtaaaa atcaaatttg gtgtgttcgt catctttctt gatactcgtt ccatcacggt
     3001 tgagatcaag ccaaattcga tcaacaatcc acctaatgac aaagttagaa taatcaatga
     3061 cacagtaggc atcatacttt cgattcctcc gcggctaagc agtgaatcga tttgttggtt
     3121 tccagtattt gaaacaaacc ctgattcgat gaccccagcc aacttcgtta aggaagtttg
     3181 tgggttttga ataaaaatca ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat
     3241 tgccggcacc tttttccatg cacacagtaa cattaaaaat aaaggaatca gtgaccagat
     3301 tgaaatagta aagtttgctt ctagaacatc agtcacttca cgaatagatg aaagacttgc
     3361 ggctttactt gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac
     3421 agttgaccac atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc
     3481 tagatttgtt gattcggata atggagacat cttatctcca aaaacggcac ctgaaacaat
     3541 agctccaaca accagcgctg gatttaagcc tagtgttgtc ccaataccga aaaatgcaat
     3601 cccaatcgtt gacataacag taaaggcact ccctacagcg ctacctacga ttgcacacac
     3661 aacaaataca gaagggacaa accactggac actgatcaat ttgaacccaa tgaccattag
     3721 agttggaatg atccctgctt gtatccatac agcaatcaat gaaccgacta aaataaaaat
     3781 aaagattggg atgattcctg gtttgatgcc attgatgatt ccttcgtgca gccaatctgt
     3841 cgggtatctt tttatcactg catacaacat gaccattccg atcgctgcta agatcgtgat
     3901 attaggtgag acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa
     3961 tacaagtgtt gcttctaaaa aacttggtgt tccttttttt cccatgataa tctctccttt
     4021 tttagttgtt cttatgttct aaaaaagtta agatatcgga ttactccgcc gcaattcatc
     4081 cgcgattcct cctttataaa aataaaaaga cctgttttaa actaaggacg aatgactccg
     4141 cggtaccacc ttaattaaaa acagatctgt ttttcacttt caaactttta ttcaaatgta
     4201 tggactttcc atgtaattcg tcaattaggc ttgatcggtt cacagcgacc accgactttc
     4261 tgaacaaaac acctaaccgc tactgcgtga aaagttgact tgtacgaagt ttaccttcca
     4321 taagatcatt tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa ttatttttat
     4381 aactttcaca cacaagatta ttttttacta gaaagtagcg ccctcttgtt tttttcttgc
     4441 tactatttct tgatgttctt gcttgattct ttctaatagc tttggctctt tcaacagatc
     4501 aagagctgta ttggcgagta gttctgctgc aatcgcaata gaagcaaggc ctttgtcact
     4561 tttagcagca gctttgaatt cttctgtgtg tccggcaata tatgtctcag agattgctac
     4621 cgtaggctgg attgttggta tgacttggct gacatttcct acatcagaag aaccgagact
     4681 tgttctttgt tccgtttcta tttcttcatc tggaacacct gcttcttttg catgctggaa
     4741 gaataagtca tcaaaagaag gagtaacgat gacatcatca actgcatttt ggaacaatcc
     4801 aaactcaaaa gtcgtgcccg tacttatcgc agcacctcgt acgatatttt ccactttttc
     4861 atatacttta tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta
     4921 atctggaatg acgttggcag ctactcctcc gtctgtaatg atcccatgga tattgacatc
     4981 ttttggcaat tgcaagcgta gagcgttgat tccattaaac acttggatca atgcttccaa
     5041 agcatttact cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat
     5101 gtcgactgga tcattcgcta aagattctgt cgttttccca taacgaaaag ctggatggac
     5161 gcataacgct acatcgacat cctcaaaaaa accttctcga acaaagctgc ctttagctga
     5221 tccgttctcg cccccttctt cccctggggt gccatataca cggatttctc caccagtttc
     5281 atcaatgaat tgcttcacta tgctggcggc taatacggag taagtaccaa acaaattatg
     5341 cccgcatgca tgaccaatac ctggtaaagc atcgaattct gccaaaaagg caattgtggg
     5401 accgggttta ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt
     5461 ttctacccaa aacctttctt tttctaattg tttaatcaac gctgctgagg aataaaactc
     5521 atagttactt acttcaggat gttcgtggat atctagagca agtgcttgat attctggtaa
     5581 acgatattgg attagatcct tcaattggtt tgttgagatt gttgttgtca taaaaaattc
     5641 ctcctatgct agtcattttt cttattttct cccgaatgtt tcccatgcag gcacgcttga
     5701 tccttttgat gttttctcga ttactttgac tgtgtcttct gtttgataag cgtctactat
     5761 tttttgatac gttttattgt tttcatcttc ttttcttgca acgatgatat tcacataagg
     5821 acgtgcatgg tcatccactg gttctaagaa gatggcatca gaagcaggat taaatccagc
     5881 atctacagct acaccgctat tgatcactga aatgtctaca tctgacaaag cacgtgctgt
     5941 ttgggaagca tctaattcag aaatgtctag attttttcta ttctcggtaa tatcagaaac
     6001 agtgggcgct tgtccttttg ccttatccac tttgatcaat ccagcagttt gcaataacag
     6061 aagagctctg ccgccatttg taacatcatt agggattgct acttttgctc cgtctttgat
     6121 atcagctaca tccttcaaag tgttagaata aattcccagc ggtgcattga cagtatttcc
     6181 gatcgatact aaatcagttc cgtgttcttt attgtaattg tctaaaaaga attgatgttg
     6241 gaaagaattc aaatcaatcg atccatctgc taaagccaca tttggttgat tgtaatctgt
     6301 aaatttcacg tattccaaat taatgttctc ctttttcaaa cggtctttca cgttgtccca
     6361 tacatctgta tcttctccaa taatccctag ttttacgata gtttcttttt tgtcagttgt
     6421 atctgctttt ccttggctac accctgccaa ccaaataatt gctacaaatg taatgactac
     6481 aaaacgaata atttttttca ttttttctcc tccactttca atgtgtaatt tttttaatca
     6541 gttgattact gataaattga cttaaaaaaa ctaagattag aatcaataat gtagccacaa
     6601 tcgttacatc tgtttggaat cgattatacc ctctcgatat agcaagattc cctaatccgc
     6661 ctgcacctac tgctccagcc atggtagtca gtccgatcac gttgatgata gtcaacgccg
     6721 agacccgaat gataccaggc aaaccttcta acaaatacac acgtaaaatg ataccgattg
     6781 gactggttcc catcgcttct gccgcttcga tcaccccagg atcaacttct agcagtgcgt
     6841 tctcgatttg acgagcaaaa aacggaacaa ctccaaaaat caatggcact aaagctgctt
     6901 tcgcaccaat cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga
     6961 taatgaaagg gatggaacga atgatattga tcagcttatc aagtatttca aatagcagac
     7021 ggttagctag taatccatta gggcgtgtca ctactaacgt cacacctaag atgattccaa
     7081 tcatcccgcc aatgaatgct gtccaaaaag acatatatag tgtttgcttc gtcgcttcga
     7141 caaattcatc cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa tcagtcattt
     7201 tatcttcctc cttcgatcac tttgatacga ctatctaaat cgtttttttg taaaatattt
     7261 atttttacac cagatttgat caaataatcc aatgcttttt tccgctgttt ggtttctcca
     7321 gctaaagtaa cgatcaaact ccctaacggc gtttcttgta ataactctac attgccatat
     7381 aaaatattcg ctgttacttg atatttgctg tacagatgag cgattagagg ttcgttcgtc
     7441 tgatttccaa tataacttaa ttccaccagc catttattgg cgatctgatc ggcaaaagca
     7501 gaatgggcaa tgatcgtttc taatgcttgg tcaagatgag tagctgttcg aataaaatct
     7561 ttggtcaatt cttcttcagg attgctgaag atctggatgc tgctaccttt ttcaacgatt
     7621 cttccatctt ccataaccgc taccttgttg cagatttctt tgacaacttg catctcatga
     7681 gtgatcagga cgattgttaa acctaactgg cgattcaatt tttttaacaa tgcaaggatc
     7741 tgaagggtcg ttttcggatc aagtgcactg gttgcttcat cacataataa taccttaggt
     7801 tcattcgcca aagctcgtgc gattgctact cgttgttttt gtccccccga cagttgttta
     7861 ggataagcag atgctttttc ttccaaaccg acaagttcca gtagttcatt cactttttga
     7921 cgacgttcct gtttcgattt tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata
     7981 gttcggcttt ccattagatt gaaatgctga aagatcatac cgatggcctt tctttctttt
     8041 cgcaattctt tgggagagag ctgactcaga ttcgtctgat tgacaatgac ttctcctgct
     8101 gttggtttct gcagcaaatt catcacacga actaacgtac ttttccctgc acctgagtaa
     8161 ccgacgattc cataaacatc gcctttttca atcgttaaat ctacagaatc tactgcctgt
     8221 atctcttttt ctttttgttt gaaacataca gaaacatctc gcaattcaat cattaaaatt
     8281 cctccttatc taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct tgaggacgct
     8341 tttttgcgtg ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac
     8401 gatcatactc tagcaagata tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat
     8461 gaattgctcg atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct
     8521 ctctgtcaaa ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa
     8581 agtcctttta ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt
     8641 tttcggaagt ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt
     8701 aacggtcgaa cagttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt
     8761 cagcttttct tacttgactc ttctcaccag ccagagcctc tctgcaaagt ttccaaactt
     8821 actttccttt tcaacgaaat cgatattttg ttgttgggat taatctacat gaggaaaact
     8881 gaaaagtcaa gtacgaaatt caaattattt tttctatatt tatttttctg tttttttatt
     8941 taacatttca ataacagttc gaagatcttt tccttccgca agaagtttca atttttcctt
     9001 ttcttcgacg gtataatttc ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc
     9061 tgctcgtgaa gcagctgttg ctcctactaa agaatcttcc actgcaaagg ctgaactagc
     9121 ttctaatcct gttcgttcca aagctgtcag ataaggaatc ggtgaaggct tgttctccga
     9181 cacttcgtct ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt gatccaagat
     9241 atctgttgct cttttcgtaa cagtggtcgt agcaatgcct agtttcatat gacgttcttt
     9301 taagtctgcc aatatttctt ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg
     9361 ttgataaaaa tattcttcac gcttcgctcg gacttcctgt actttttcag ggctacctgc
     9421 tgctttcact aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat
     9481 ttcttttggg atatggatat caaattgctg aaatccccaa agccagccat cacgatacgt
     9541 tttttctgtg tcaactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt
     9601 aatcctttcc aaaacaatac acatagttta gcacgactgg tccaaaaaga acagaagcaa
     9661 agaaccagcc ttctcgttta taagagttct tggaatcgac ggatatagat tttcttcaca
     9721 gctgtcacaa gcgccagata agcgatgact gtagcaaata accataacca gtaattgcca
     9781 ggaagtggca tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac agaaccgaca
     9841 ataattccta atgtagtgat aacaaacatg gcaaatgttg cgttgctttg gataaatggt
     9901 actttcggtg tacgcagtgc atggagcact aaggtttgtg tccatagtga ttcgacaaac
     9961 catcctgcat ggaataatgc gatgaaaatc actcgctgtg aactatccaa tgtatggaaa
    10021 cttccgccca ctacagcagg acaaataaca aaatacatca acaagtaagt agtaatatca
    10081 aatatcgaac ttgttggacc aaagtaaacc atgaattccc caatactaga tgcattccat
    10141 ttttttggct cgtccaaata ttctttatcc atccgatccc atggcaagga aatgcaggaa
    10201 acatcataga tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg
    10261 aacgtacttg caatcagcac ggaaaacata ttgccaaaat tagaacttgc tgtagccttg
    10321 atatatttca tgatattccc aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc
    10381 gtcaaatctt tttctaaaag gatcacgtct gcagattctt tcgcaatatc cacagctgta
    10441 tctacagaaa taccgatatc agccactttc attgcaggag cgtcattgat accatctcct
    10501 aaaaatccga cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc tttttgctga
    10561 ggattcaatt ttacgaatac ttcatggtct tcagctactt gtgctaattc atgatcactc
    10621 atttccgaga ttttctcacc ggtaaccaat tcattgatct ctagacctac ttgacggcac
    10681 actgaacgtg taactaattc gttgtcccca gtcagaactt ttacagctac accatgttct
    10741 ttcagagctt tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa agcaagataa
    10801 ccaatcaaaa ccatgtctga ctcatctttg actgaaaatt ctccaacgac gcttggattg
    10861 gtcttttgtg cgacaccaat cactcgtaag ccatcttcat taagtgactg tacttttgca
    10921 agtacggctt cttttcgttc ttttgttaat ggtgtaacag taccgccaag atcaataaat
    10981 ttagaaatgt tcagcatttc ttcaatcgca ccttttgtga tcatttgtgt tttaccagct
    11041 ggatcagcaa ccacaacact catacgacga cgttcaaaat caaaaggaat ttcatccact
    11101 ttttgataat tgatcttatc tgtatctaaa gtttctgttg cttcatcaat gatagccaca
    11161 tctaacaaat ttttcagccc tgtttgatag tagctattta gatacgcatg gcgcaaaaca
    11221 cgatcatctt catttccttc acaatccata tgatattcaa gaatgatttt atcttgtgtc
    11281 aatgtccccg ttttatccgt acataacaca tcgattgcac caaagttctg gatggcattc
    11341 aaatttttga tcactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc
    11401 gtaacgatca ttggaagcat ttcaggtgtt agaccaactg ctacagatag tccaaaaaga
    11461 aaagcttcta gccaatcccc ttttgtaagc ccattgatga cgataactgc tggagccatc
    11521 aatgacataa atttgatcag tagcatggat gttttatgga tgcctaattc aaagcttgat
    11581 tgaattggtt tttcagatag cgtttctgct acatgaccaa atagtgtttc atttccaaca
    11641 gaaacaatga ttccttccgc acttccactg atgacattac ttcccataaa gacaagattt
    11701 tcgtaacttg tttcgctttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga
    11761 tagctttccc ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga caataaacgc
    11821 atatccgctg gtatcatatc ccctgctgaa agccgaacaa gatctccaca gacgatttct
    11881 tccataggaa tctctgtgta ctttccatta cgttttacag ctgccgttac tttcacaagt
    11941 gatttcagtt tttcagccgc ttgattggaa cgtacggact gaatcaacgt cattgtcccg
    12001 ctgacgataa ccatagtgaa gataatggct acaccgaaaa gatctttatc tccaggagct
    12061 gctaatacat attccgtgat gaatgaaata attcctaatg cgatcaacac taatgtaaat
    12121 ggcgtgatgt atgctttcaa tatttctaca atgaatggtg tttttttgcc atatgaaatc
    12181 gcattttcac cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct
    12241 gatgcatgga atagttcaaa taattcttgt ttcgtttttc cagcaaaagc tgcatagatt
    12301 ttttgtgtat ctttcttttt ttccatctca atctctcctt tgtgtccaaa agacagctgt
    12361 tctcgctgct tttggtcttt agctattcat cggatcgata gagaaaaaac tgaaaggggc
    12421 tgagaacaga tagataatag ccctggcctt tcctctagat ccttgttttt cgaatgactg
    12481 tcatccattt gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt
    12541 ttcgaatagt tatttttcca attagaccac ctccttttta tttttcaccg atggggattt
    12601 gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa
    12661 aaaagcttct cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatacc
    12721 tgcttttagc taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt
    12781 ctctcgacgt ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatctta
    12841 ttcggttttc cttcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg
    12901 tttttcatac tttttttaaa atacacacgt tatattcctg tctcacgcgg ttttatttgt
    12961 ctcattttcc acctcccgct ataatagaaa tatagtagga aacgaggtgc tgttatggaa
    13021 agaacacttg ttattatcaa gccagacggc gtgagacgcc atttagtcgg aagcatcatc
    13081 caacgttttg aagcaaaggg actggccatt gctgaaatga agtttgaaac tatgactcct
    13141 gaattagcaa aagaacatta tcagcacttg actgagcgca gtttttttga tgaactgatc
    13201 gattacatga cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat
    13261 atcgtccgta agatggttgg agcaacaaaa gctgccgatg ctgttccagg tacgatccga
    13321 ggagactacg cgctgccggg gactgaaaac atcattcacg cttctgattc ccgtgatgct
    13381 gcggtaaaag agatcgcccg ctttttcccg ccattcgata taaacaaacc gtctgaaaaa
    13441 gcggcgaagt gatacaaatt ctaaactgct ggtctagttg ctgggttcag caatggccag
    13501 cagttttttc tctaaggacc tccgttgatt ctttcctaac atatattcga gcactgtgaa
    13561 gaattcttgg tagtctttcg tcttttcttc ccctaaagca gcagaaatca atctttgata
    13621 ataatctgcc atcaatgttt catagatcac tgtacttctt tctttatgtg ctaagctccg
    13681 tttcatatct tcattcacac tacgtgcctt tttcaaccga ccattttcta tataaaacga
    13741 agtcaggttc attaaaaaag taaagagata ctgctcatga ttgttcaact gtgctaatcg
    13801 tcgcattctt tttttgattt tcaaataatt gttctcaatg aaccgatcac tgaatatcca
    13861 aggaaaatca gctagaatag atagttcaaa ttgaccatag tctggtatct gcatcaagta
    13921 ttcttcaatg attcggcatt cataacgtaa aaattcaaat tctgtctcag atacttctgt
    13981 tttttcgacc atgtgaagat tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg
    14041 cctacggtta ttgctgatct ctttataagg atcaaagctg tcttcttgta cccgatctag
    14101 cattcgtctt tttttcttta actttattgg ttcatccagc tggtttcgca tccattcata
    14161 ttctgcaagt gtcatgttca ttcgctctaa aaatgatctc aatgttacaa atgacatttt
    14221 tttcactgaa tttgtctcaa tcccgactag agaagtctta aaacagattc cttccgctaa
    14281 ttgggactgt gtcaacttct tgttgactcg aagtttcttg atcaattcac catcatacat
    14341 aatagttctc ctttttaatt taaatttttc cacattcaat aaatagatac gttaaaaagg
    14401 aaaaatacac gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa ccttcaagca
    14461 ttagaatgtt gtctaactac tgaaggttgg tttgattgtt tattgattat ttttttgctg
    14521 ttcaacgtct gttcttgtta ctggatataa gttggttgtt gttttattac cattttttga
    14581 aaagacactt gtcgaatgtt cgcctttttg cttttcgatt gcttcattac gttccgcttc
    14641 atttttatag tcatattttt tcggatcaat cgtctcaaat ccgtttggca cataaaaccg
    14701 caagagattt tcctgattca gcttatctga aagacttaac gcagtcttga ctttttcctg
    14761 atctttggag atttcctcat cgattcctgc tgctttcgta tcgatgattt ctcctgtctg
    14821 attattgtaa gcttttcctc ctaggacggt gtatttgggt gtcacaaagt tcccatttcg
    14881 aaaagcaact acttgatcat gctgagaaga taataagtct gtaccaaaat gaagataatc
    14941 tttatcatcg attcccaata gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta
    15001 ttcatgattg actgtccctt tagtataacc aggcatatgg atcatcaatg gcactcgttg
    15061 catctgtgcg ttgtcaaatt catcccatgt atctggatct ttaccaagag cgcttgctaa
    15121 atctttatta tctgtattcg agatcccaaa atgatccccg taaatcacaa agattgaatt
    15181 ttggtaaata ccagattttt tcaaatacgt aaaaaattgt tccaatgatt gatccagata
    15241 atgtgccgtt cgaacataat tatctactgt gctattgcca gtattcaaag atggaaagtc
    15301 gaaattcttg tcatccgtat aataaggcgt gtggttggtt acggaaagaa atttcgtgta
    15361 aaacggctgt tgtagatgct caagatattg ggcagattct ctgaataaat ctttatctaa
    15421 tatcccataa cctaacgttt cgtcggattc atcaaaataa gatcgatcaa agaaattgtc
    15481 ataacctaag tttttatata catgatctct attccaaaaa ctagctacat tcccatggaa
    15541 aacagcgctt gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt
    15601 gtctgaccca agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa
    15661 catattttca gcatcacttg tctttccctg tcctacttca tgaaagaaat tgtcaaagct
    15721 gatcgttgct tgattctgga aaatactatt taaaaacggt gtgacttctt gtccgtctac
    15781 cttcatatta atcaaaaact gctggaaact ttctaaatgg agaacgatca catttttccc
    15841 tttagcgatc ccaaatgttt cgggatttgg ctctgcataa tgtttctttg tataatctaa
    15901 aacttcatca atcccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt
    15961 gattccgtca taaatagtat acgcatcgat tcctagatat ttgacaatat atgaacggtc
    16021 aaatgttcgc tgcagcaatt gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa
    16081 aataagccct gaaagacagg taacagcgat agccatcggt ttgccgacag ggttgctttt
    16141 gataggtatt ttcttgacct tcaaccaaac cagtagccca atgaacacag cgatatccag
    16201 ccagtagatg acatcatggg gcttcatcaa agaaaaggag cttcctgaca atccttgcga
    16261 cactttcgaa aagcctaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa
    16321 aataatattc aagtacaaca aaagcgtatt taaaatatca atgatcaaaa gaatagctaa
    16381 cgctggcttt ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagtcgcaat
    16441 tgggttgatc cacaagataa agtactggat cgtaccttcc acacctaatg aaaaatctgc
    16501 atagtaggca atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa
    16561 acgattttgg cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt
    16621 tttcttattt tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt
    16681 tttattcttt ctaaagatta ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa
    16741 gcgattaaac taatgaagag ataaaagtct tgtggtgacc gcttcactcc acggtattca
    16801 aaagccagtt tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc
    16861 tccttatttc ttgatgcagg acagcttttt cacaacttct tccttaaacg gtgttctcct
    16921 gccaactcct cggccacaag tcacaatatt tataaaacat gagaagctgt tttttgaaat
    16981 tgttccttgt ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa
    17041 tcagctgacc ctcggtatta gattatgtca ggacgctaat tttacaacct cttggtaaac
    17101 ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat ataaggagct
    17161 attttttgaa attgctcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata
    17221 cacggtgttc aatcagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
    17281 tattttgccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctctgttt
    17341 ttcattgtcc ataggattga tattgtcctt gtgatgactc attcgtaata attgaggctt
    17401 gattttcttc acttcccgtt atcgtttgtc cactgccaga atctgaagga cttgttcctg
    17461 tcgactcgtt tttttgagaa gagacagttg ttttcttgac ttgttcattt gtctttttcc
    17521 attccgctaa acgatcggcg accacttttt tgtctacttt ttcagaataa gcggtgatca
    17581 cttttccatt tttcaaacgg gctactttgt cgccatcatg agtcaaaagc aaaacggtca
    17641 aatgcttgtc tttaaattga tattgtctga gagaaatcgc actaagtacg tcatcgatat
    17701 taataaattg attttcttct tttccttttg gcaaataaat gtccaattcg cctttttcgt
    17761 aattggcagt tattttatac tccgcccacc aataagttga gaattctttt ttgaaattat
    17821 ctactgattc tttgcttgct acgatttttt ctgtttcttt ctgagcaaga tctgctgtca
    17881 tttttcctga aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga
    17941 gaagaaacgg tctttttttc ctcatgcttt cctccactcc tatttaattt tgactattct
    18001 attatataca aaaaatgaaa tagaaagaca aaaaagttga aaaagatcgt aaataattga
    18061 acttcttttt cacaaaatgg taagaatgga gaatttcacg catttgctct caactattct
    18121 ttcttaagcc tcaaatccgt ttttatctga tacgtctttg aaccaatgac ccgatttttt
    18181 gattgttctt tttccatctt gtgctaagtc tactgaaata aagccatatc gattcttata
    18241 tgcattggtc cacgaccagt tatccataca tgtccacata tgatagccta aacaattggc
    18301 accttcttga atggcttgat ggacatattt cagatgagat tggacaaatt cgatccgata
    18361 atcatcgtgg atcatgccat cttctttgat aaatcgtgct tcgttttcta cacccatacc
    18421 gttttcagaa ataaagcagt cgatatttcc ataatgatct cgtacattga tcaaaatatc
    18481 atagattcct tgttcataga tctcccatcc gcgataagga ttcattttac gaccaggcat
    18541 ttcataattg tcgaaataat cctcaggcat tggaccgttt ttagtatcga ttggtgtttc
    18601 ttttgctttt acccggcgtg gctggtaata attgatacct aaaagatcta ctgtattttc
    18661 tttgataacc gtcaaatcct ctgcatggat cacaggcatg agatcaagtt cttttactat
    18721 ctcaatcaat tcacttggaa actcgccttt tacagctgga tcaaggaatg aacgattgaa
    18781 gaacgcatct gctatttgcg ctgctttaac atcttcaggg tttgattcgt ctcttggata
    18841 gcttggtgtc agattcaaga tgataccaat aacaccacct tgctccattt catggtaaga
    18901 tttgatggct aacgctgaag ccaacgcttc atgatagcca acttggatcg cttttttcat
    18961 atctgactct tctggataat gccatcgata taaataacct gcttcgactg gaacgattgg
    19021 ttcattatga gtaaaccatt tttttacttt cgaaccaaac aattcaaaac aacttttggc
    19081 atatttttca taggcttcaa ccgtttctcg gttcaaccat ccgccttttt cttgcagata
    19141 catcggcata tcgaaatgat acagattcac aaagggttcg ataccgtttt caatcaactc
    19201 atcaaaataa cgatgataga aacgaacggc ttcttcgttg attgttccag attcatcagg
    19261 tatcaaacgg ctccattgga tagatgttcg aaatgaatta tgcccagtct gtttcatcaa
    19321 ctgtacatcc tcttgatact tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc
    19381 aaagaacttt tgcggttctt ttttgtacca ataatcaaaa atgttctctc cttttccatc
    19441 gcctgaaatc cttccttctg tttgtgggcc acttgctgca gaaccccacc aaaaatttgc
    19501 tggaaattga tattttttca tcaaagcacc tctttttctt ttttagttcg ttttagttga
    19561 ttccatgtat gatattttcc atcacaatca ctttttcatt ttcttcctct tcttttttga
    19621 ttcgttttaa cagctgttct gctgctcgtt ccccgatttt atctgttggt ggcgtgatcg
    19681 tgattttcgg aaggatactc agatcgtttt tatctaaata atcatagtga ctgaacgcaa
    19741 tatccttccc gattctgaca ccttcttgat tgaaacattc gatggcacca atcgacatat
    19801 tgtaattgct gacaaaaata gcagttgccc ctttttctaa taatgctttt gccccgtaat
    19861 agccgctttt tctagaatag tctcctgtaa agacctctaa ttggatcgat ggatcatttt
    19921 ggaccgcttc tttggctcct gctaaacgtt ctcttgctac gtagtctgtt tgagtggcag
    19981 caataaatcc gattttttta tgtccttgtt caccatgatg agcgatcact tttgctacac
    20041 tttctcgatc attcactaat attgtatcaa tacctgctgg tccttctgga caattcaatg
    20101 cgataactgg aatactcatt tttgtgagca tttcaaatcc agaccactct tcttcggata
    20161 agaagacaat cagcccatcg atcacatgct tcatcaggta ttcaattttt tgttcaattt
    20221 tttctggctg atcggcaaac ccgcttaata ataagctata cccattttct tcagctactc
    20281 tttcgatccc tgagacaact tccgacccaa aacggctgga aatcccgttt atcaaaagtc
    20341 caatagagaa gcttcgatta ttcttcaacc ctttagcaat gatgttttct ttatagtcca
    20401 acgctgaaat cgcttctgct atttttttca tgttcttttc cttcagttgc tgaccattca
    20461 agtaacggga aactgttccc actgaaacac cagccatctt agccacatca cggatcgatg
    20521 ccatacattc tcactcctgc cagttacatt tgatttctct ttcttgttgt tcgattatga
    20581 tgaccgcatt ctttgcatag tgttgcttgt catcaatatc catataacta aagtcttcag
    20641 tagagcaact tcctctcact attaattata cttaaacaag tatttgaacc ggttcatttt
    20701 taatatagca tttttttaaa agattgcaac acttttttaa aacgctttct gaaagtggcg
    20761 gattttttgc ctttttaaaa ttttattcta aaatttactc tatttgtggg tgttttgtgc
    20821 gtttttctct tccatccgtt tgaagtttta cattccaatg gtataatttc tttatcctat
    20881 caaatggggg aagtattttg aagaaattaa tcgtactatt tattttgtct gcgggacttt
    20941 tgacattgac tggctgtgat ttgtttggcg gctctgacca atcctcggac gaaacaaccg
    21001 attcttctgt cttaaaagcg gaaaaagcag ataccaccaa cagtgtatat acgctaaaag
    21061 tacgtaaaga tatggattta tccgcgggac agttgacatt tacagataaa aaaatggaat
    21121 ggacacgcac gtatcagaca caaggaactg attcttctga atcatctgaa gaaaaaacag
    21181 tgctgacaga tatcaagatc actaccaaag atgacgaata tattatttca ggaaaagaaa
    21241 ataaaaaaga aacaacagtc atatttacaa aaatcggtaa ttaccgtttg aaggatgagg
    21301 atggcaacat ctacagtcta tagtgccgtc tgttggatct tcttttgttt ctccccttct
    21361 tttctgctat agtaaagaag caagatgatt gtaaaggaga cgaaatggat gatcgatagt
    21421 cctgattatt gtacatgtaa taacaacaaa aatatccata cagaatttaa tgaccaagga
    21481 agcttgagca tatgcgacaa ctgtcacaaa ctagtcaaag attcatttaa aacatttgaa
    21541 tcttatcctg ttcaaaatta tgtaaaaagt agaagaagat aaacaaatcc aagcaaaact
    21601 cactatggct gatttttatg aacttcaaac tcagcttctt cctttgatca agcttcatca
    21661 gtaaaacaaa cagtggatca tttcagttta caaatcaaag agtaaagatt tatttatttc
    21721 aaatatttga atataaaata accaaacgat agaaaggctt attctacatc acgtag
//
LOCUS       DAZUMT010000048        21585 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000048 DAZUMT010000000
VERSION     DAZUMT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.048"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..966
                     /locus_tag="ACG8FM_001583"
     CDS             265..966
                     /locus_tag="ACG8FM_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5094246.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1575..2591)
                     /gene="tsaD"
                     /locus_tag="ACG8FM_001584"
     CDS             complement(1575..2591)
                     /gene="tsaD"
                     /locus_tag="ACG8FM_001584"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS5094247.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2588..3052)
                     /gene="rimI"
                     /locus_tag="ACG8FM_001585"
     CDS             complement(2588..3052)
                     /gene="rimI"
                     /locus_tag="ACG8FM_001585"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5094248.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3059..3601)
                     /gene="rimI"
                     /locus_tag="ACG8FM_001586"
     CDS             complement(3059..3601)
                     /gene="rimI"
                     /locus_tag="ACG8FM_001586"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5094249.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3585..4307)
                     /gene="tsaB"
                     /locus_tag="ACG8FM_001587"
     CDS             complement(3585..4307)
                     /gene="tsaB"
                     /locus_tag="ACG8FM_001587"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS5094250.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4498..5478)
                     /locus_tag="ACG8FM_001588"
     CDS             complement(4498..5478)
                     /locus_tag="ACG8FM_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5094251.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5502..6986)
                     /gene="galT"
                     /locus_tag="ACG8FM_001589"
     CDS             complement(5502..6986)
                     /gene="galT"
                     /locus_tag="ACG8FM_001589"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS5094252.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6998..7987)
                     /gene="galE"
                     /locus_tag="ACG8FM_001590"
     CDS             complement(6998..7987)
                     /gene="galE"
                     /locus_tag="ACG8FM_001590"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS5094253.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8235..8402
                     /locus_tag="ACG8FM_001591"
     CDS             8235..8402
                     /locus_tag="ACG8FM_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS5094254.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8464..10275)
                     /locus_tag="ACG8FM_001592"
     CDS             complement(8464..10275)
                     /locus_tag="ACG8FM_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS5094255.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10272..10637)
                     /locus_tag="ACG8FM_001593"
     CDS             complement(10272..10637)
                     /locus_tag="ACG8FM_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS5094256.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10800..11195)
                     /locus_tag="ACG8FM_001594"
     CDS             complement(10800..11195)
                     /locus_tag="ACG8FM_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5094257.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11213..12175)
                     /locus_tag="ACG8FM_001595"
     CDS             complement(11213..12175)
                     /locus_tag="ACG8FM_001595"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS5094258.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12175..12387)
                     /locus_tag="ACG8FM_001596"
     CDS             complement(12175..12387)
                     /locus_tag="ACG8FM_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS5094259.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12408..13106)
                     /locus_tag="ACG8FM_001597"
     CDS             complement(12408..13106)
                     /locus_tag="ACG8FM_001597"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS5094260.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13126..13668)
                     /locus_tag="ACG8FM_001598"
     CDS             complement(13126..13668)
                     /locus_tag="ACG8FM_001598"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS5094261.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13800..14804)
                     /locus_tag="ACG8FM_001599"
     CDS             complement(13800..14804)
                     /locus_tag="ACG8FM_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5094262.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14801..15790)
                     /locus_tag="ACG8FM_001600"
     CDS             complement(14801..15790)
                     /locus_tag="ACG8FM_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5094263.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15787..16593)
                     /locus_tag="ACG8FM_001601"
     CDS             complement(15787..16593)
                     /locus_tag="ACG8FM_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094264.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16759..17715
                     /locus_tag="ACG8FM_001602"
     CDS             16759..17715
                     /locus_tag="ACG8FM_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5094265.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17792..18310)
                     /locus_tag="ACG8FM_001603"
     CDS             complement(17792..18310)
                     /locus_tag="ACG8FM_001603"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5094266.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18398..18547)
                     /gene="rpmG"
                     /locus_tag="ACG8FM_001604"
     CDS             complement(18398..18547)
                     /gene="rpmG"
                     /locus_tag="ACG8FM_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5094267.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18775..19221
                     /locus_tag="ACG8FM_001605"
     CDS             18775..19221
                     /locus_tag="ACG8FM_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS5094268.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19416..21311)
                     /gene="asnB"
                     /locus_tag="ACG8FM_001606"
     CDS             complement(19416..21311)
                     /gene="asnB"
                     /locus_tag="ACG8FM_001606"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS5094269.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6506 a         4564 c         3710 g         6805 t
ORIGIN      
        1 attgaaaaaa tttttttgaa taaattaaaa aaataaatta tattatcgtt gttagcgttt
       61 actatatgta ttattgttgt tttttataca caaatcattc gcttcaaaac aaattattca
      121 ataccattgt atatttttta cataataaca aattatattt gtaaagtatt aaaacttgtt
      181 tctaatgttg gtatgttaaa actttacaaa ttctttaaaa aaagtgacaa taaatatagt
      241 gaacaataag gagaggactt tttaatgcgt gttaataaaa tcgattttca cttttcaaaa
      301 ctcagatatc aacctgattt tgaacatttc tcggatgaag agttccaaca gatcaaggaa
      361 catactgttc ttcgttctta taaaaaagga cagatccttt tcgacgatgg tgatcccaga
      421 caacggttgt atatcttgac caaaggactc gttcgtttag aacgttatga tgaaagtgca
      481 acttattact actatgacta tgtgaataca aataaccttt ttcctatgtc aggaatgttt
      541 ggagaagaga cctacactta tacagctcaa gcgatgactg atattgaaac attctacctt
      601 cctgtttcct actatgaaaa attagcacga aaaaatcccg aacagttgat ctatatgata
      661 caaaaaattt ctaaagtagt tgaaatacat gaattgcgta tccaaatcgg attgacctct
      721 agcgcctttg accgggtaaa acaaaattta ttcattttga aagaagaatt gggcgtacca
      781 acaaaagatg gtcatatcac cattccttat ccgatcacct taaaagaatt agcagtgaat
      841 agcggcacga cacgtgaaac tgctggacaa gtaatcaaac aactacgtga ttccgggctg
      901 ttagattatg acaaaaagcg ttttactttc tgtaagacag ctactactcc agctatgaga
      961 gaataacgcg aacaaaaata agcagtgacc ctgataaata tcagggtcac tgcttatttt
     1021 cctccctaaa acttctgaaa gatccggcag ttttagatca ctggacaaga ctcaaagatt
     1081 ttctatgtgt gtttcagtta tgtaatattc actgtttgca aaaaatactg ttagattaac
     1141 tagatgaagc gtcttgtcct catccgatct agattattcg atattcctcg acaataaacg
     1201 attactcagt gagggcaatc agaggaacgg ataatgataa ataagctgta aaaaatagca
     1261 aaataacaat aatgattaaa agtatgtaaa aaatagggct ttgacaaaaa cgtgtcacag
     1321 ccctatttcg attaaacggt gtgtacctgt caactcttcg gtcacaagtc ataatgttca
     1381 taaacatgag gagctgtttt ttgaaattgt tccttgtgaa tcagagttaa acgacaggct
     1441 tacgaactct tgattaaacg gcgttcaaaa gcttgcatac gcggtaataa gaacagccag
     1501 gtcaaaaaca tgagaagccg tttttcctgg gctgttctta ttacttgatg cagggcagct
     1561 ttttcacaac ctctttattc agataaaacc aagcttggat cagcattcaa ctgatagcta
     1621 gcaaaatttc ttttttgcat ttccacatgt gctgctgctc caatcattgc agcattatct
     1681 ccacaaagtc gaagaggggg aatcactagt tctacctcag ggagtttagc tgccaaagca
     1741 tcctgcaatc cttcacgcaa cccttgattg gccgctacac ctccagctat aatcagttgc
     1801 ttgactggat aattttgaca agctcgaagt gttttagtaa tcaatacatc gatcacactt
     1861 gcttgaaagc tagctgctaa atcatttttg tctagatctt ctccacgctg ttgtgcatta
     1921 tgcaccaaat tgataaatgc actttttaat ccgctaaaac tgaaatcata attatcttca
     1981 tggatcatcg ctcgcggaaa atggtaatta tcttttccct gatgagctag ctgatcgatt
     2041 tcttttccgc ttgggtaaga aagacctaat actcttccaa ctttgtcata agcttctcct
     2101 gctgcgtcat ctcttgtctc cccaatgatt tcatatgatc catcttcttg catatagact
     2161 aattctgtat gtcctccgct aacaagaagt gccatcaatg gaaactggaa tggtttaacc
     2221 agacgggctg catagatatg ccctgccata tggtttacag gaataagcgg caattggtgt
     2281 gcccaagcaa aagccttagc tgcagaaata ccaatcagca acgagccgac aagtcctggt
     2341 ccataagtga cagcgactgc cgataaatct tctgccgaca caccagcttc taccagtgca
     2401 tcttccaagc ataaggtgat ttgttctacg tgatggcggc tagctacttc aggtactacg
     2461 ccaccaaaac gtttatgact gtttatttga gaagcaacga tgttagataa gatctctgtt
     2521 ccatttctaa cgaccgctac actcgtttca tcacaacttg attcaatcgc cagaatcaat
     2581 tcttcattca tttcatcgtc tccgttttta actttctgct cataatgatc gcatcttccg
     2641 tcggattgcg ataatatgat ttccgtttac ctaatatacg aaacttttct gattcataaa
     2701 gttttcttgc tgcttcatta gatagacgta cttctaaaaa aacatgcagc gaatctttta
     2761 tttgttccgt aatcagtaat tgtaacaaat gccgtgcatg cccttttcct tgttctgaaa
     2821 ctgcaacagc aatatttgtg atctcgactt catctattac tttcgagtat ccaataaagc
     2881 cacagatctg gttatcttca gctaaaagca gataatcaga gtgaggctgt tggatgtctg
     2941 ccaaaaactg cattttcgtc caaggtgaac catgtggata agcattttcg ctaatcatcc
     3001 atacgcattc tgccaagtac gcttcatcaa aatctttttt tctgcaaatc aactaatctc
     3061 actcattttc taaaagtttt atcatatcca acgcatcttc ttttgtttca gtgtaatatc
     3121 tttttttgat cgttgttgct tgaaacccca atttccgata aagtctttgt gcatcttgat
     3181 tactcatacg cacttctaat gacatcgtct cacagcgatt cattatagca aagttctcga
     3241 tttcgtcaat aagcagcgaa ccgattcgtt tacgctgata tgccggtcga acggcaatat
     3301 tcgtgatatg gcaatctaaa ccgaacgctc ttgaaccgat aaaaccaatc attcttccat
     3361 tatcaaagat acctagatat agatgaggaa gcggtgactt caattctgat ataaaggcac
     3421 tttttgtcca tggcaattcg ccattataga cttctcgttc gacatccagc agctctttga
     3481 tatcttctgt agtcaattct cttagtacgt aacgacgatc gatttgttcg actgtttttt
     3541 ctgtataaat cattttatct tgaaaaaagc ttctggcata cgctctaaat tttttcaaca
     3601 taactttcgt ctccaggtgt gtgcgtctcc aaccattttt cttctgcttc tactcgtttt
     3661 aaatagcgag gaagaaaagc ttgtttctct gcaactggtt caactaaagc acctaattga
     3721 gccacagtta caccacttgg aatatcccat gctggaactg tattaatcat cgcactaggc
     3781 aatatttcct ttatttcaga gaagaatttg tgacaatcac tcccgacgaa ataaatagct
     3841 tcattcagct tttgtagttg tgtgaacaaa tcagaagcag aaatatgtac atcttcaagt
     3901 acagtttcta atctattttc tttccagcgg tatgccccag cgtatacatt ttttcttcga
     3961 gcatcaaaca tgggcacaat aatattatcc acaccgatgc aattagccgc gatggttttc
     4021 agactagata ctcctactag ctctttatct aatgtataag caatcgtttt tgctgtcgtt
     4081 acacctatac gaagccctgt ataagaaccg ggaccatcag aaaccgcgat tcggtcaatg
     4141 tcttttggac ttatttgaag atctgcaaac aattggtcga ttgcaggcat cagtgttaca
     4201 ctgtggttgc gtttgatatt tgtttgtatt tgtccaagaa tttgttcgtc ttctaccaca
     4261 ccgatcgcca aagtttggtt tgcagtatct attcctaatg taatcatata tttgatcctt
     4321 tctgctatga cattctcgcc ttccattgta gcatactttt ttattctgag gatgaaaata
     4381 ccaaaggcaa aactagtaca ataatacatg attatctgct ggcgggcaaa ttttgcccct
     4441 tcctgaaaca caaaaaagct gcggtatcac tccattttcc cacagctttt ccatctttta
     4501 tttcgttgat tctcgaataa tcaactctgt ttccacaata atctttcttg aaaccggtgc
     4561 tttggaatta cttaattgaa gaatcgtctc cactgctagc tctcccatcc attcggtata
     4621 aacttttaca gtagaaagcg agggcgttac atatttcgct acactgacat cattgaagcc
     4681 catcacagaa atatcttctg gtaccactaa acctttttct tgaatcgctt tcagtgcgcc
     4741 aatcgctaat gcatcattag ctgcaaaaag cgcatcagga acatgcggat tcgtttttaa
     4801 atattgcttc actgcttgat atcctgactc gacagaaaaa tctgcttcga taattggacc
     4861 ttcgtcgtag cctttttctg ccaaaacttg ttgaaatgcc ataattctgg gatcttttag
     4921 ccgctgatgg tttttcttcg tatattctgt tccagcaagc atagcgatat gttgcgcttt
     4981 atgggagaca aagtgttcaa tgactttttg aacacttgac ttaaagtcga caactagaga
     5041 gtcgattcct tgagaagtgc cgtcagaatc taccaaaagc aaggcatttt gggatccttt
     5101 catttctaat aactgagctt cgtcgaattt tcctagtgcg atcgttccat ctgccggaag
     5161 atcactaatg gaatcccacg tttctttcaa taaagtaacg tttgcttctt ctgccttttt
     5221 ttcgattcct aggcgaatag ataaataata aagatcttca agctcttccg tatcgtcgta
     5281 ccactggatc aggcgaagcg tttgcttagt atttgctgca tttttacgat gtttcgtata
     5341 atttaagcgt tctgctgctt caaaaatttt ttgctttgtg gctaagccaa cggaaagatc
     5401 aggatcatag ttgagaactc ttgaaacagt agcaggagaa actcctgcca actgcgcaat
     5461 gtctttaatt gttgccattt gtctctcctc cttttttacg tttataaaat cgcagtaaat
     5521 cgttcaaacg ctgcctgtcc tttttcatct cgcttgaata ctccagcgtc ttccagtaca
     5581 cgtgcaaaaa cctgacctac agatttttgt acgacatcgg tgaccgtatc tttcgtcaaa
     5641 tcaggatgtt ctttttttaa ttgttctgcc cacggacgat gatattcagc catctggttt
     5701 tcttctccta ataagaaatg ctctacttct actagttctg gtttcaaacg aggtggtaaa
     5761 acagcaagcc ccattacttc gatcaaccca atgttctcct ttttgatatg ttggacatct
     5821 ttatgtggat gaaaaatccc atcaggatgt tcttctgaaa cattgttgtc tcgaaggaca
     5881 aggtccaact caaagtaatt gtcccttctt cgtgcaattg gcgtaatcgt atggtgtggt
     5941 gtaccatctg ctgaaaaagc tctgatagat actgtttcat ccgaatatgc tttccatttt
     6001 tccaatactg agttagctgc ttcaataagc aagtttttct ctgttgattg caaacggatc
     6061 acagacatcg gccatttcac gactcctgcg atgacttgcg gataatcttc tagtttgata
     6121 agtttttcta tcggtgcttt tgccatagga aattcatgac gtcctgcctg ataatgatca
     6181 tgagaaagaa tcgatcctcc aacaattggt aagtcagcat tcgaaccaac aaagtaatga
     6241 ggcaagactt cagtaatccg aagcagtcta gcaaatgttt cctttgtgat tttcatcggg
     6301 cggtgttgtt ctgataaaat gatcgaatgt tcattgtaat aagcataagg ggaatattgg
     6361 aatccccagc tttcaccgtc tagattcatt cgaataatcc ggtgattggt gcgggctgga
     6421 tagttcaagc gacctctata tccttcgttt tccatgcaaa gcatgcaggt aggatagtct
     6481 acttttaccg cctcgcgttc tgctgcgatt tgttttggat ctttttctgg cttagataaa
     6541 ttgattgtga tttccagttc accatactcc gactctgccg gaaatttgat gttttttgcg
     6601 atggctcttg ttttgatgta gtcatttttc ttacttaaat taaagaaata ctcagttgct
     6661 tcctctgggc ttttcgaata gtgctgtgca aagaatgcat tgacgactga aggtggcggt
     6721 gtgagaaaat ccattaattg cgcttcaaaa atctcttgtt ctgctggcaa ttctgaaaga
     6781 acaccatttt ctcttgcctt agcgacaagc tgatccaata agtctacaga ttcttcctta
     6841 actggtctta cttcaatgct ttctagagca tcttctccta ccatccctaa aagacgattc
     6901 tgtaaatata agcggtccat ttccatccat ccgccagctt cgatcgcaag tgtggcaaaa
     6961 tcacaaatcg tttgactgat tgacatatct cattccccta ttcttcataa ccatttggat
     7021 ggctgacatg ccaatcccat gctgttttga taatcgcttt gatatctgtg tattcaggtt
     7081 tccagcccag aatctctctc gctttttcac ttgaagcgac taatcggcta ggatctccag
     7141 ctcttcttgg tgctatttct gctggaattt cttttcctgt cacttctcta gctgcttcta
     7201 gcatttcctt gactgagtat cctttgctgc tgcctaaatt gaagaagttg ctttcatttc
     7261 cttctttcaa gtattccaaa gctaaaatat gtgcagcaat caaatcttct acttgaacat
     7321 aatctctgat acatgttcca tctggtgtat cataatcgtc tccataaaca gccaaggctt
     7381 ttctttgacc aagagctact tgtaaaatga ttgggacaag atgcgtttct ggtgtatgat
     7441 cttctccgat agatgcatct gcttttgctc cggctacatt gaagtagcgc aaagcgacat
     7501 agcgcatgcc atatgcttgg tcacaccact tcatcatttt ttccatcatc aatttacttt
     7561 caccatacgg atttttagga ttagctggtg tatcttctgt gattggagac tctttcggct
     7621 ctccataagt tgctgcagta gaagagaata cgattttatt cacgttgaac tcgtgcatga
     7681 cttcaagtaa gatttgcata ccgtatacgt tgttgttgaa atacatcaat ggtttttcga
     7741 cagattctcc tactaaagag cttgcagcaa aatggattac tccttcaatc ggttcctttt
     7801 caaatacgga acgtagaaat tctttatccc ggatgtctcc ttcataaaaa tgagcatctg
     7861 gatgtactgc ttgtttgtga cctgtgagca aattatctac cacagctact tgatacccct
     7921 tttgaactag ctggtctaca gcgtgcgaac cgatataacc ggctccgcct agtactaaaa
     7981 ttgtcataaa aaacactcct ttgattagta aaattgtgtt tgttgattct atataattta
     8041 aaaaacgaag tttactttta tggtgaaaac aaacaaagta aaatttcgta aattcatatg
     8101 gtaaatttat aataaaacat ttagtaaaca ttttcaaggg ttttttgcaa aggtttacaa
     8161 acaaacgtaa aactagcctt tttttctctt ttgttccata atagatataa caaataataa
     8221 atgaggtgga ttcaatgaaa aaatttgtat ctggaatatt agtaggaagt ctggcaacag
     8281 ccgctgccgt tgctggtttg gttgcttctg tgaaaaaaac agtgatcgat ccaattgacg
     8341 aaaaagaagc aatgatcgaa gaaaaccgta aaaaagcaat gcgtaaacgc gtttctcgtt
     8401 aataattatt taaaaatacg catgaagact ttctaaaaaa cagataagtc atcatgcgta
     8461 tttttatttg tcagtcaata taagttgtgc tattttatct gcctgtctca gattccatgg
     8521 atctgtataa ggtctagcta ctgctgcagt tagccaatcc acaccttcaa caggtattcc
     8581 atagcaataa aagtcagact ggatttcccc tgaagcatca atcacgcgat tcgtacctcg
     8641 ctcaacatct atagcttctg aatgataact tcctgtatta tctgaaaatg aaaaagggcg
     8701 aacaagtttg ttttccttca aattttttat gacaggatta cttgaacgag aaaagcttgt
     8761 ttgtggcaat cgagcttcaa tcaaatagcg actgctgatt tctttatctt gtagagaaat
     8821 aacatagcgg ttgtgctttt gctgaatatc aaatggcggt tcgatcaaat gaaaaatacc
     8881 cgcatctatc agtgctgcta attctttcgt ccgaatgatt ggcggaccga tcgttaaaaa
     8941 gctatttaga tgagtaaacc atccccatag tatttctttg tattccttag cagaaaaaac
     9001 gtgccattca atagcctgcc ggattggatc acgccagtct tttaatgcat caaatgttga
     9061 agcaaatgct cctgtgcaat tgcctttttc cgcttctttt atctgccctt caataaatat
     9121 tcggctagct tctgaaaagg gctcattctg ataggcaaga ggagtttcta aatacgacca
     9181 atcccatcta taagggatta gtgctggatg ctttccgaga cactcctctg aagagaggga
     9241 gagaaaatca tgctcaaaat ttttctttgg aatcggcaag tgatattctt cgatgatttt
     9301 cttgtaataa aacaactcaa cgtctttttt cagatagtca aaaaatgttt cgccagtcaa
     9361 aaatcccgac tgatgccatt gctccaggtt ttcttttgtt agtaaccgtg gccatgccat
     9421 tgctccgcct tgcttttgat ttcttcctcg agggtaataa gggagccccc ttcttgatcc
     9481 actgtataca atcggttcct tgcctgatgg atgatagacc agttttccct tttcgttctc
     9541 gaagcgccct cctcttgata ctgtaaacaa tccgacataa tcaaaaaaac ttaagcctag
     9601 acctcttaaa aagacatttt ctttaggcgg tatcttcgaa agatctacat ctgcaggatt
     9661 agaaggaagc tgatacagta aaacattttt ctttgcgtag tctacccaca ctctttcttc
     9721 ttctgtctca tcattttccc agtgaccgct tgctaaaaca agctgatcga tagaatagct
     9781 ttgctgatct gtctgtaaaa gataaccatc ttttcttttg cttacttttt ttactaaaga
     9841 ataaacaaac tgaatagaag ctggaaattc ttcttgcaac tttgagaaaa accagcgttg
     9901 atagagtccg tataaacatc gagtagattg ctgatctttt tccagacgat tgatttcttc
     9961 tataattagt tgcttatttt tggttaccgt ctgttcgata taatctgtag caggtccttt
    10021 actccattga aacaagttgg gtccctgtgt taccaatcct ccactggaca atgtttcatc
    10081 tgtaaaaagc gtcacttgtt gagaaacaga attcattaat aactccggtg gctgattcaa
    10141 ccgccaaact cgacctccag gaccattggg atcgaaaaga aaaagttcaa gagaatgtct
    10201 gtcctgatta ttatggagaa tcctttctgc tacacttaat cctctaggtc cggcaccaat
    10261 gatccctatt ttcatttgca tgcctccatt tcgataaatt ttttcaataa aacagcatgg
    10321 ttctgatgtt cattttttgc accataaaca aaagtaatga ctggatgttc tttcataagt
    10381 tgactgagtt taaaaatagc ttgcttcttt tcttcttcga aaatcaattc ttgctggtat
    10441 ttctttgcaa atgaagcaaa tcgttctggt acatggtcaa aagttttgcg tgcttcatta
    10501 ctaggtgcaa tttctttcaa ccacagatct aactgcgctt tttcttttga aattcctcgt
    10561 ggccagaatc tatccacgag tattctatag ccatcctctt tttcagcatc aaggtaaaca
    10621 cgttttaatt tgatcatagt tgtttccttc tttctacctt ttcgcttagc ttatatatgt
    10681 agataatagc acaaaataaa aaaaatgaac ggcacaacta tccattgcgc cgttcatgtg
    10741 atcttggtca aaatctaatg attgattcgg tttttaaagc tgtcatacaa tcatctgttt
    10801 tatacttttt tacttttgat ttttccagac catccgtcat atccaccctt aagtaaataa
    10861 atctcttgat aacctgcttt acgtaattta tttgctgtac ggatgctcaa acttttcttt
    10921 tgatcgtaaa tatagacagg ctggtctttt cgaatagatc ctaatgaatc tttcaaaaca
    10981 gtgtaaggga tgtttcttgc acctaaaata tgcccagcat caaacacatc tttttcgcga
    11041 acatcgatta cttgtgcttt tctcattcct tctcgaaatt cttcttctgt caaagtcgtt
    11101 gctgaacgtt tggccattac tcgtagatag acttcccaaa ggatgattgc aagcacaatc
    11161 aatagcaaaa tgatattaat tacccataaa accatgtaat gcctccgttt ttttaagcaa
    11221 atttcaaggc taatgaggca gcaccaatga caccagcttc attaccaagc tctgctaatt
    11281 tgatttttgt tgattctctt acttgcggga aagtaaattc tttaaaatat ttctctacac
    11341 ggctacgtaa aaattctcct gctgccgata ctcctccacc taacacgata cttgacggat
    11401 ttaatgtatt ccccaagttt ccgcaagcta aacctaagta gaagcatacg cgatcaacaa
    11461 ccatcaatgc aaacggatcg tcttcttgag ccatttcaaa gatatcttta cttgttactt
    11521 cttctccgtt gtctagcatt gctttcagtt ttgaatcccc tgcgtattct tctgccaaga
    11581 aacgacctag gcgtaccaca cctgtagcgc tagatacagt ttctaaacat ccgcgtttac
    11641 cgcaagtaca ttcgaaccct tcaggatcca cagtgatatg tccgatttct cctccagctc
    11701 cgctcacgcc gtgaatcaaa tttccgccag caacgatacc tccgccaaca cctgttccta
    11761 gagtaacgaa tacaacgtct ggatcgtttt ctccagcacc tttccatcgt tcacctaaag
    11821 cagcaacatt cgcgtcatta tcgatagcga atttgattcc tgttcctgcc tcgatcagtt
    11881 ttttcacaaa ttgaacttct gtccagttta agttatacgc accaataacc gttccagctt
    11941 cgctgtccac gcttcctggg gtacccatgc cgattccgat aaagtcttcg gctttcatat
    12001 tatataaatt caaacgatga ttgattgatt caatgatttc aggaacgata tgcttaccat
    12061 catccaaaat attggtatcg atgctccatt tttgctgaat ctctccttct ggtgttaaaa
    12121 ttgcaaattt tgctgttgtt ccacctaaat caatcccaat aatcttcttg tccattacaa
    12181 atttcctttc tttcggcttt cttcaattcg gtgctctctt tttaatatgc gccacgctgt
    12241 catgtatgta tcatgatcaa tcaaacggct atcatacaga ttacgcaatt cgatagtcat
    12301 taattcaatg tcatagatgc gtgcacccac atagataaag atgccaaatt ttttgaacag
    12361 ctgctgtaca tcatataagc tctccatctt taagcacttc cttcatatta tacaagtaaa
    12421 ccatattttt ttaaacccag taaaatacat attacaaaaa gaaatacaaa aactagtgta
    12481 gaaagaatcc tttggtgtat cgaatagctt gtctgttgtt tcggtagacc caaaacattc
    12541 cctaacacta atccgccaat tagcccaccg acatgtcccc aaacatctac agaggtatcc
    12601 agaagaccaa aaacaaatgt caacacaata aataaaagaa aatgtctgac tagctgctgg
    12661 atcgctgggt tatgtttaaa atgaaaccct aagataaaca acgcaccgaa caaaccgaat
    12721 atcgaagtac ttgcaccacc agacagaaca ttcgcttcgt taaaagcaaa gcttgctgta
    12781 tttcccatta tcccgctgaa catataaatc aagaaaaaac gccaatggcc atagattgcc
    12841 tcgatttgct gccccataaa atacagtatc agtgagttca ttacgaaatg catcagtcca
    12901 aaatggatga agattggtgt cactaaacgc caccattcat tgaagtgaac aagataagga
    12961 ccatacattc cggcataata agggatctca agtcgcggaa ataattccat catgaggaag
    13021 accgctgtct gtatccctaa aaaagcatag gtccataacg gtcgtcttaa ccatgcacgc
    13081 aattttattt gctgttgata attcatgaaa acctcccatt tcattttagt caataaacaa
    13141 ttgttggatc ttctgatcaa agctctccgg ttgccattgt tcattcaatt gttcacgaaa
    13201 aacaaggcta catgtttttt tatgaaaatt atttaaatac cggtcgtaat aaccaccgcc
    13261 aaaaccgatt cgataaccgg ctctattgta gataatacct ggtacaatga ggagatcgat
    13321 catctccggc aaatagagct tctctgattg tggctcaaga acaccaaaac tcgaacgtac
    13381 aagaacggac tgttcatcca tttcataaaa cgccatctga cgtttcggca aagttttagg
    13441 tacaactatt ttcttttgtt ctttttctgc tcgttggata accggctgtg tatccagttc
    13501 aaattcattc gataaagtcg tccctatcac ttttgctgac ttccagtact cgctggcgaa
    13561 tagctgttta tagattcgct gttctttttc ctttttcttg ccacttttgg ccagattttt
    13621 cagcttttcg attcctttcc ggcgtaatct tactttcaat tggtccaaaa actcaccctt
    13681 ccttctctca taaaatggta taggaaaaaa agaaaaatag caatcgaact ttcaaataaa
    13741 tgcataaata gaataaaaag aagacctaga aatgattggc cttccttttt taatgaattt
    13801 caactgttca gcgcgtgttt ttgtctgata aaaagtgcat acagaaaata tggcgcacca
    13861 aaaagggcaa tcaaaattcc tgtaggtact tccccacttg gtagaatcac tctagcaacc
    13921 atatctgcac taagcagaaa aatggaaccg aacaaaccgc tcagaacaaa ggtccagtta
    13981 ttttctttac gtacgactag cttcgcagca tgaggagcta gtagtcctac aaaagataaa
    14041 ctacctgtca ccgctacact accgcttgcc aaagcaacag ctaacaacaa aaggattttt
    14101 tgacttttag ccacgttaag gcctaatcct tgcgcgcgtt cttgaccaaa tgatagcaat
    14161 acaatcctct gtgcataaag tcctcctagc ggaattaaga taaccgccca tggaagcaat
    14221 gcccaaacat aaggccagct cgcaccccag attgatcccg ctaaccatgc attcacaaat
    14281 ccataatttt ccttagataa ttggatcgtg catagcaatg ttaatgcaga aaggccggca
    14341 ttaacggcaa taccggacaa taagattcga ttcataccca aaaagccatt tcgatcatag
    14401 gccgcaagat agacacagca agcagctaaa acaccaccta gaaaggctac aactagcaat
    14461 agaaacggca gcgaatagtg cgaaaagaaa cctaaataaa gcatcactaa aaatcctgct
    14521 ccggcattaa tgcccaatat actagaatct gccaattcat ttcttgttac tccttgcaag
    14581 atataaccag aaacgccaag acaagctcct gctaataaac tgatcagtaa tcgaggaact
    14641 cgaaattctc taacaatcag cgccaaagaa gatgattctt tccctatgaa cgtggcaaaa
    14701 agctgatcca ggtaaataaa aggcgtacca atcattaaac tacctataga caggatgaca
    14761 agtacaaaaa gcaagaaata gaacgtccaa tatatttttt tcaccgttgt tccctcctta
    14821 cttgtatcag taaaaacgga acgccaatca atgcaatgac taatccaaaa ggtgtttcaa
    14881 aagggggatt gatcatcctc gctgtaaaat ccgccaacat aaccaatcca ccgccaacta
    14941 aaaaagagac aggtagaaca taacgatagt ccgttccaac cattccgcga acaactggcg
    15001 gaaccaacag cccaataaat gaaacaggtc cgactaacga aacggcaatg gctgctaaca
    15061 ctgcaactag acagaaaaca atcaaccgcg ttcgtttagg atgacttcca agagctaatg
    15121 catgtgtttc actcaaatga atcaaagata ttgcttttcc taatgaaaaa gaaaccaaaa
    15181 cagcaagtat atataaagga aacaatactg ctaattgttt ccatgtgata ttggcagctc
    15241 cgcctacaaa ccaaaaagca agatcctgct gcagattaaa gaattgggta agtgcttggc
    15301 tgatagcagt aaaaaaagaa ctaatagcta cgccagctaa aatcagctgt aaaggattca
    15361 tgcccatttt cgaaaactgc aaaaaaccaa atacgagcaa agttacagca aaagaacctg
    15421 ccaatgacat tagaaacaca gaaaaatcag aagctttagg ccaaaaagca aaactaaggg
    15481 ccattgccaa cgaagcaccc gcgttgattc ctagtaagcc tgaatctgct aaaggatttc
    15541 ttgtcactcc ttgcatcaat gcaccgctca atgcaaaaca actccctaca agaaatgctc
    15601 ccgccacacg aggcaaacga atatttcgta tgatttggtg actttgtttt gatctatcaa
    15661 aatcaaacaa cgcctgccaa aaatctgctt tttccacagt cgctgcgcca tttgaaagag
    15721 caacaaaaaa aataatcaaa agaacaaaac agaccaaaag caggagtcta tttttcttca
    15781 tagtcctcat aattgatcct cctgctcttt tggcaacaaa tcataggtta gaaaaactgg
    15841 tttatttgtt cttggaagcg tgacaatcgt tgcatcaatc tcaaatattt ttttcataca
    15901 ctcttttgtg atcacttctt caggtgtacc ttctgtgatc aacttaccgt ttttcattgc
    15961 taataaataa tctgaaaaac gtgaagcatg attaatgtca tgaatcgaca tcaagatcgt
    16021 tgtttgtttc atttgattga tttcagtcaa taactgtagg atctctaatt ggtgtgccgg
    16081 atctaaaaat gttgtcggtt catccaagat caagatatct gtttcctgag caagcgccat
    16141 agcgatccag acacgctggg tttgtccacc agacagcgtt gcgatatccc gcatacgaaa
    16201 tccagctaga cctgtagcat ccaacgccca ctcaattgct tcatgatctt gcgtgtccat
    16261 gccactgaac agtttttgat aagggtatcg tccgtaagat actacttctt ctactgtcat
    16321 acctgtaggg tgttcactag tttgtgctaa taatgcaatt ttttttgcaa gttcttttga
    16381 actgtatgta gagatatcag agttattgac aatgatcttt cctgatttca caggtagtat
    16441 acgggtaaaa cttttcaata aagtagattt tccacttcca tttggcccaa tcaaactagt
    16501 gattttccct tcaggaatcg taacagacat gttttcaatg atttttttgt tttcgtagcc
    16561 ggctgcaatg tcagctgctc ttaatctcgt catttcaccc atcctttcgc tgaaaatcat
    16621 tctcaattat gacgctgttt caacatttcg tcaagcgcta ctttcatatc tatagccaaa
    16681 tgaagcggtt gacagcgttc gaaatatcag gaataataac caatgagaaa gattctcaat
    16741 tatgttagga ggaaagaaat gaaattaaaa acaaaagtat tcacattatc tagcatattg
    16801 ctgctggctg gagtactgag cgcttgctcc ccctcttcag ataaagaaac agctacaact
    16861 gccaattcga cgacttcatc caatagtact catacagtca cagatacact tggacataaa
    16921 gtagagattc caaacgcgcc caaacggatc atcggcagtt acttagaaga ctatctgatt
    16981 gcactagggg aaaaacctat cgcccagtgg actgttggca gtggttccat tcaagattat
    17041 ctgcaagatg atttaaagga tgtcccaacg atttcttatg accttcctta tgagaaagtg
    17101 ttaagctttg agcctgattt gctgttgatt tcttcttccg ctactgtcga aggcgggaaa
    17161 tacgaacaat atagcaaaat cgcgccaact tatgttgtaa aaaacggaac agatgtcaca
    17221 tgggaagaac aactaaaaga tgtcggcaaa gctttggcaa aagaaaaaga ggcagaaaaa
    17281 atcatttccg attatcaaaa atccatcaag aaaactcgag aagaactagc agataaaatc
    17341 gagaacaaga cagctgcagt gttgtgggtg accaacaact cagcctttat ggtcagcaaa
    17401 aatcggtcaa gtggaagaat cgtatacgac gatctggaat ttggtgttcc tgatctagtg
    17461 gaagaggtct ccaaaaaagc cacttctgac tggtctgctg tttcctctga aaagctagct
    17521 gaattagatg ctgactacat catccttgtg aacagtgaca aagatgctgc catgttcaat
    17581 gaaccaagct ggaagaatct aaaagcagtg acagacaatc atgtactgca attcggacct
    17641 gaatctagtt ggctatataa cggacctatt gccagtcagg aaatggtcaa tgatatcaaa
    17701 gaaaaactaa attgatttta actaaaaaaa gcatcttcac gattagtcgt gagatgcttt
    17761 ttttcttatt aacaaactag acgcatagat tttattcttc ttttgaagca accctatttt
    17821 ttactctttc ccatttttga aaacgatggg catatagatt ttcttgatcc gttatcccct
    17881 catactcctc tataagggcg aagtctgaaa aatcaatatt cccaatatac gtatcgcctt
    17941 caaacgtgct atcgatcaat gttcgccaga tccgaccagt gtaaggaaga agttcttgaa
    18001 aaatccgact accacccgaa acatagatat cttctgagat cgtttgacta tacgacaaaa
    18061 gttcatcgat cgaatgcatt acttccgcat tttctttcgt gcgaaaattt ggttgcgttg
    18121 tcaacacgat gatatggcga taaggaagtg aaagatcccc cattccttca taggtctttc
    18181 ttcccatcac taaaattcga tccattgtat gttcgcgaaa gaaacgcata tcatttggca
    18241 atcgccatgg taacaaacca tttttgccga tcagaccatt tttatcttgt gcccacatgg
    18301 aaataaacat attatccgct ccttatgatt ttagatgttt tgagataaaa aaagtctcaa
    18361 ttctttgacc agcaaggaat tgagaacttt aaaaacatta ttttgtttca cggtgcaaag
    18421 taactttgcg ttcgcgtggg caatattttt gtttttccaa acgatcagga ttgttacgtt
    18481 tgtttttgct tgttagatag ttacgttctt tacaagaagt acactctaaa gtgatgttta
    18541 cgcgcatgtt tttccctccc atttttacaa gaatcctcta agatgactct cagacttgat
    18601 tatcttacca tgattttttc tagattgcta gtgtttttca cgattttgtt aagtaaaact
    18661 acttgacgat aaatacaacg gttttacttc tcaaacaaaa tctttttctt tttgtataaa
    18721 tacccttaca cttgtttata tagattatgt tttttaataa agggggatac taatatgcca
    18781 tggaacatga aggactttcc tgcatcaatg aaaaacttag ataaattgac aagaaaaaaa
    18841 gcaatcgaca tcgcaaatgc attattagat gaagggtacc ctgataaccg tgcgatccca
    18901 attgccattg atcaagcaaa aaaatggtat gaagatgcta gtgaatcaga aaaacgaact
    18961 ttcgaaaaag aaaaaaatcc aagcaagacg gatgaacacg atacgaatcc tcgtgctgga
    19021 aaactgcttg attcagatgt gatcgtagaa tatgaggaag aacaatggat cgtgaaatct
    19081 aaaggggcaa aaaaggcaag caaccatttt gatacaaaaa aagaagcaat cgaaaaagga
    19141 aagcaagtag cacaaaacaa agaatcaact ttgttcattt ataaaaaaga tgggacaaaa
    19201 gaaaaagaaa tcgattattg aacatatgta aaggggttgt gacgaaaatc gtgtcacaac
    19261 ccccttatgt tcgaaacaat ggtgttcctc tgccaactcc gaaccataag tcataatatc
    19321 tataaaacgg gaagagctgt tttttgaaat tgtttcttat cactcagtgt taaacggtag
    19381 gagaaaacct cttttttatt gatatttgat tttcatcaaa cagtcacata gtcgattgct
    19441 tctgcttgcg gaatcgtttc atctacaaag tagactttat accatgtcaa gaaactgaag
    19501 attgtccaga tttttctttg ctcttcttta cgtccttcgt gatggtcatc tagcaacttc
    19561 atgatttttt cttgatcaaa aaattctttt gcaaaatctg cttcgaataa tgctttgact
    19621 tgttcgtagc cgtgttcttc tttcaaccaa gctttgattg gcacagggaa tcctaatttc
    19681 acacgattcg accattcttc cggaagatgt ttgttcgcag cttgtctgaa aatatctttt
    19741 gtgttgtttt gattcaataa aaacttactt ggtatttgtt gagccaattt catgacttcg
    19801 atatccaata aaggtactcg gacttccaaa gaacttgcca tagaaagttt atctgccttt
    19861 aacaagatat cttttggcat ccattgatga agatcgacat attgcatctt attgacttca
    19921 tctttgattc cttctgtttt ctcgtaatga acagtcatga tttcatccac agatggtgct
    19981 gtttgatatt ttggtgttaa cacttctgat gcttctgatt cttcgaacac tcgagcatga
    20041 ccgataaaat attcttttgc aggagcaagt gattcatata aatggatccg tccatggaag
    20101 tttttcattt ttccgatttt acgtcctaaa ttatagcgtg ttccttttgg taattttttc
    20161 aatccttgag caatcacacg gatgaatttt gaattcgtat ggaatccata cgtttggtat
    20221 cccgcaaaca gttcatccgc cccttctcca gattggacta cccgcacact ttgtgaagca
    20281 agatttgcta aaaagtacaa aggtacgcaa gaaggatttg aatcaggttc atctaagtga
    20341 tactggatca aagggaatgc tttgaacgac atgtctccgt cgatcactgc tgctgtatta
    20401 tctaagtcca atagttcggt tagttttctg gcttcaatcg cttcattata ggttttatca
    20461 tcaaacccga ttgagaaaga atgatctggt ttcaatacag aggttacata actggaatca
    20521 accccgctgg ataagaaaga accaacttct acatcactga ccgtatgtgc ttcaatagaa
    20581 gcttgaacgg tttcgtcgat tttttcgatc cattcttgtc gggaatgcgt ttcttttgtt
    20641 tcaaaatctg catcccaata ttgctgaatg tctatttttc catctttata cgtataataa
    20701 tggccttcag gcaaacgata gacatctttg aagaatgttt cgccgtttaa tggagaatat
    20761 tggaaagtca tataaggttt caacgcttct ttgttcaatt ctttgttaaa atcaggatga
    20821 ggcagaaaac ttttgatttc tgaaccaaac ataaatgttc cgttcatttc tgcataataa
    20881 tatggtttga tcccaaagtg gtctcttgca ccgaataact cattcttttc attatcccaa
    20941 atagcaaaag caaacattcc gcggatcttt tgcaataatt ctgttcccca ttcttcgtaa
    21001 ccatgcaaaa ggacttctgt atctgcatgt gtttgaaaaa cgtggccagc tgcaatcaat
    21061 tcttctctca aaggctggta attatagatt tcgccattaa aaataatgat ctttgtccga
    21121 tcttcattgt aaataggctg agtaccgccc tctaagtcaa taatacttaa tcttctgaat
    21181 cctaaagcaa catgtttatc gatatattct cctgaactat taggtccacg gtgaacgatt
    21241 cgatccatca ttgtattaat aattgttttt ttgttgtcct tgtcgttaac aaaacctaca
    21301 ataccgcaca tttaactttc acatcctaat ataaaattac tgtttatttc attcatatgc
    21361 atcttatcat agaataaata gcggacgaat attttctctc ttacaatttc gtcacgtgaa
    21421 aacaaaagat gaaagtttct gtttttacag aaaataaagt cccaatatct aatttaccat
    21481 gttttctttt ttgaggctac cttattctct tcgtgttaaa aaaatattag tggatgctat
    21541 ttccaaaaaa accagcaaaa aaagacacga cctaaagatc gtgtc
//
LOCUS       DAZUMT010000049        21302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000049 DAZUMT010000000
VERSION     DAZUMT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.049"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8FM_001607"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8FM_001607"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS5094270.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8FM_001608"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8FM_001608"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS5094271.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8FM_001609"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8FM_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS5094272.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8FM_001610"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8FM_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS5094273.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8FM_001611"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8FM_001611"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS5094274.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8FM_001612"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8FM_001612"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS5094275.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8FM_001613"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8FM_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS5094276.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8FM_001614"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8FM_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS5094277.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8FM_001615"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8FM_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS5094278.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8FM_001616"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8FM_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5094279.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8FM_001617"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8FM_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS5094280.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8FM_001618"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8FM_001618"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS5094281.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8FM_001619"
     CDS             15402..16376
                     /locus_tag="ACG8FM_001619"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5094282.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8FM_001620"
     CDS             16546..17946
                     /locus_tag="ACG8FM_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS5094283.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8FM_001621"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8FM_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5094284.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8FM_001622"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8FM_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5094285.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8FM_001623"
     CDS             19454..21253
                     /locus_tag="ACG8FM_001623"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS5094286.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLFQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5925 a         4668 c         3840 g         6869 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttttca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ct
//
LOCUS       DAZUMT010000050        20481 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000050 DAZUMT010000000
VERSION     DAZUMT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.050"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(383..1849)
                     /locus_tag="ACG8FM_001624"
     CDS             complement(383..1849)
                     /locus_tag="ACG8FM_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5094287.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2140..4224)
                     /locus_tag="ACG8FM_001625"
     CDS             complement(2140..4224)
                     /locus_tag="ACG8FM_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5094288.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4366..4488)
                     /locus_tag="ACG8FM_001626"
     CDS             complement(4366..4488)
                     /locus_tag="ACG8FM_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094289.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(4749..5108)
                     /locus_tag="ACG8FM_001627"
     CDS             complement(4749..5108)
                     /locus_tag="ACG8FM_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS5094290.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5138..5479)
                     /locus_tag="ACG8FM_001628"
     CDS             complement(5138..5479)
                     /locus_tag="ACG8FM_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094291.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5476..6150)
                     /locus_tag="ACG8FM_001629"
     CDS             complement(5476..6150)
                     /locus_tag="ACG8FM_001629"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5094292.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACG8FM_001630"
     CDS             complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACG8FM_001630"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS5094293.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8664..9854)
                     /locus_tag="ACG8FM_001631"
     CDS             complement(8664..9854)
                     /locus_tag="ACG8FM_001631"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5094294.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9875..10402)
                     /locus_tag="ACG8FM_001632"
     CDS             complement(9875..10402)
                     /locus_tag="ACG8FM_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS5094295.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10449..13238)
                     /locus_tag="ACG8FM_001633"
     CDS             complement(10449..13238)
                     /locus_tag="ACG8FM_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS5094296.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13385..13585)
                     /locus_tag="ACG8FM_001634"
     CDS             complement(13385..13585)
                     /locus_tag="ACG8FM_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5094297.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14038..14358)
                     /locus_tag="ACG8FM_001635"
     CDS             complement(14038..14358)
                     /locus_tag="ACG8FM_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094298.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14861..15979)
                     /locus_tag="ACG8FM_001636"
     CDS             complement(14861..15979)
                     /locus_tag="ACG8FM_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094299.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16212..16334
                     /locus_tag="ACG8FM_001637"
     CDS             16212..16334
                     /locus_tag="ACG8FM_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094300.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            16450..17757
                     /locus_tag="ACG8FM_001638"
     CDS             16450..17757
                     /locus_tag="ACG8FM_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS5094301.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17878..19077
                     /locus_tag="ACG8FM_001639"
     CDS             17878..19077
                     /locus_tag="ACG8FM_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5094302.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19104..20372)
                     /locus_tag="ACG8FM_001640"
     CDS             complement(19104..20372)
                     /locus_tag="ACG8FM_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS5094303.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5929 a         4358 c         3572 g         6622 t
ORIGIN      
        1 aatggtgttc aatcagctga ccttcagcat tagctcaaaa agcggcaaaa tatgaggagc
       61 tatttttccg tttgtttctc taatactcag gtcaaatcgc tgattttaca acctcttcct
      121 taaacggtgt tccaaagctt gcatctgcgg taataagccc agccaaacga aaaatatgag
      181 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
      241 gattcacggt gttccagaag tagcttcttc gaaaaacaag ccgaaacttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
      421 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacgattg
      481 gtgtgatgat attcgtaaat aattggaacc accatgcttt aggatcagca gtataatcta
      541 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
      601 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
      661 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
      721 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aaaatcggca
      781 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
      841 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
      901 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
      961 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     1021 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcactagca aacagcatac
     1081 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     1141 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     1201 cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     1261 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     1321 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     1381 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     1441 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     1501 catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt tgtgccaaat
     1561 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     1621 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gatcctaatt
     1681 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     1741 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     1801 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     1861 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     1921 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     1981 ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt
     2041 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     2101 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct ctttttttac
     2161 atgcaagctt cgcatggcat tcaaaacagc taatactgtc accccaacat ctgcaaatac
     2221 tgcatctccc attgatgcaa aaccaaaagc acctaaggca agtaccaaaa tttttatccc
     2281 aatagcaaag acaatgtttt gtttgacgat tttcaatgtt ttctttgcta aatggattgc
     2341 ttctgcaatc ttcgatggtt gatcattcat gatgactaca tctgctgctt caattgcagc
     2401 atcgcttccc aatccaccca ttgcgattcc tacatctgct cttgctagca ccggtgcatc
     2461 gttgattcca tctccgacaa atgcggtctt tttattgttt ctttgtaaga tttcctctaa
     2521 ccgctgcact ttatcttcag gaagcaattc gctatatatc ttgtccacgc ctacttgctt
     2581 cccgatatga tcagcaattt ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc
     2641 agcctgtttt aattgaacca atgcgtctac ggcatcttct ttcaaggtat ctgcaataac
     2701 aagataacca ctgtaacttt gatcgatcgc tagataaagc aatgtaccga tttcttgact
     2761 taaaggaaaa gagatttcaa atctttccat caatttgtga ttacctgcta atacttgttt
     2821 gccatcaact tcagctaaga tcccttcacc tgcaaattcc tggatattag ttgttggtaa
     2881 aagcggacct gcgtagcctt ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc
     2941 tgcactcgca atatattgca tgaatttctt tggatcgatt tttgtatcga tcgtctggat
     3001 cgaaaagtca cctttcgtta atgtgccggt tttatcgaat accactgtct cggtttgcgc
     3061 aagcaactcc aagtaatttg aaccttttac taatacaccg attttacttg cgcctccgat
     3121 accgccaaag aaactaagtg gcacggaaat cactaatgca catggacatg agatcactaa
     3181 aaatgtcaat gctcgataga tccattcaga aaacgccgct ccagaaacaa caagtggagg
     3241 taaaacagct aataaaacgg ccaatcctac aacgactggt gtgtaatagc gcgcgaattt
     3301 agtaatgaaa ttttcagctg gtgctttttt actgctggca ttttctacta attctaataa
     3361 tttgctgata gtagaatttt cataagattt ttctacttga actaaaaatg gtttattttt
     3421 attgataaat ccgcttaata ctgtttcacc aacatataca gatttaggga ctgattctcc
     3481 tgtaagtgct gacgtatcga ctaatgattc tccttcgatg atcgttccat ctaacggtac
     3541 acgttcacca ggcttcacaa gtacaaactg accgatctgc acgtcttcag ggttcacggt
     3601 catcaatcct tccgtcgtct gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg
     3661 aatcgatttt cttgaatgac tgactgccaa tccttggaaa tactcgccga tttgataaaa
     3721 tagcatcact gctacggctt ctggatattc accgatcaac atagcgccaa tcgtagcaac
     3781 agacataaga aaattctcat caaagatttt tcctttgacg atattcatca ctgccttctt
     3841 cactacatca tatcctatca gcagataaat caataaatac cctatcaagc gaattggttc
     3901 attaaaatca aataatagca ataaagcgat acctgcaagg gaaaataaaa tccggaacag
     3961 cgtatttctc tctttttcat ttccatgttc agatactttt tccttatcta tttctgttac
     4021 tttgacatct ggttctaatt ccttcactac tttttttgtt tcttgttcaa tcgtagccaa
     4081 atgatcttcc tctgcgtcaa tcgttagttt tccagaagtg aagtttaccg ttgcttcctt
     4141 gactccattg atcttctgaa ctcctttttc gattttcatt gcacagttcg cacaatctaa
     4201 accttctaat ctgtaacttt tcattttaat gacctcctcg cctcaacata tgaacatctc
     4261 ttcataacta attataaatg aatggttatt catatgtcaa ggtatccgaa agaaaaaatg
     4321 aaaaacatct gcatcggaaa atttcttcca tacagatgtt ttggcttatt ctttcttttt
     4381 ctcttcgatc attgcccctg ctcttatcca acccgcactt gccttcttga tcttgacagg
     4441 atagcaacta agtttgaagc ctgttgctgg caatttgtct aaattcatta atttttcgat
     4501 aggacaatat cttttttcaa tccctgcaaa atgtccttcc cagatattcg aagctccact
     4561 acgctttcaa aaatatagaa agagttcttt aatgaaaatt atttcaactt tccataaaca
     4621 gtaatcccat ataaataaaa gacgtaaaag tcacatggga gaacttatac aggaaaacac
     4681 atagagtcag gtgaaataca tgtatcccac ctgactctat gcgttttcta acctttatta
     4741 ttctcttttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa
     4801 gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca
     4861 tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac
     4921 atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc
     4981 ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct
     5041 gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa
     5101 caactcattg tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt
     5161 ttattcttct ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt
     5221 ccgtattttc ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca
     5281 cccgttcttc tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg
     5341 ttacagcaac gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta
     5401 cgaatgattc tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg
     5461 cccaccgtat ttccatcatt tctcactttt ctttcgctca tttttatctc cactaaaaaa
     5521 atcgactgcg gcaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt
     5581 tgggtggacc ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt
     5641 gaatatgcca tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat
     5701 ttggatttta gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac
     5761 aaacggctcc atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt
     5821 tcgataaacg gattcaaatt gctgcgcaga tttctcagtt ccgaatatag cctgatcgct
     5881 agtattatgc caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc
     5941 taaacaatct tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg
     6001 gttcatgcat tcaaatttca tcgttggata ttgggagatt agttttccgg caatcaaaag
     6061 tggataacct ttacctagat cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct
     6121 gtcaccgata aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga
     6181 tgattttctt ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta
     6241 taaaaaatga caccagccta gtcccttcaa tcaattgaat gaacgctcat caagtgtcta
     6301 gttttttata ctttttttct taacgtgccg gattcccctt gattccttcg ttgtttggtc
     6361 cagagaaatc gatcacacct aagcttccgt caatcagacc actgatgacg ccatcagaac
     6421 atatatttat aggctcatct aagacttcca gtaaatgttc ttttactaat atctcttcta
     6481 gattgcttgg gatagattgg tcataacttt atctggtccg atgatttcaa ggatgaatta
     6541 aacttacata tggatcattg catgctgaaa atacggacct actccatctt cgaatgttac
     6601 tattagtttg ttttcaggta ctagatgctg ctttatttca acagcagaag catcatcaaa
     6661 tcagatttcc atttgaataa ccttctttgt atatgtttga ttgattgcga tttcgttata
     6721 gaaccttgtg aatcaaatag caatttcttt tgctttcttc tcttgtctgc tggaaacttc
     6781 ctttatcccg tattttaaaa aaagaactgc gagagttcgc agttcttttt tttaaataaa
     6841 cgttgctctc cggccaactc ctcggtcaca agtcacaata gtcatcaaac atgaaaaact
     6901 gttttttgaa attactcctt gtgactcgga gctgaacggc aggcgcacaa cctcttgata
     6961 taaacggtgt tcaatcagct gaccctcgat attagctaaa aaaacggtaa aatatgagaa
     7021 gctatttttc cgttttgttt ctctaatact caggtcaagg cactgatttc acaacctctt
     7081 ccttaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
     7141 gaaagccatt tccacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaacct
     7201 ctccactaaa cggtattcaa aagcttacat ctgcggtaat aagtcccgcc aattcaaaaa
     7261 catgaacagc tgtttttgct tggctgttct aattacttga tgcagaacag ctttttcatc
     7321 acctcttatg gataaattcg gttcaacaaa cgtgggaatg gaatagcttc acgtacatgg
     7381 tcgataccag caatccaagt gactgttcgt tcaaggccta gaccaaaacc agagtgcgga
     7441 acagaaccgt atttacgtag atcaagatac catgaatatt ctttttcatc caaaccgtga
     7501 ttacggattt gttctagcaa gaaatcatag tctgttgccc gttcgcttcc gccaataatt
     7561 tcaccgtaac cttctggtgc gatcatatct gcacaaatta ccacgtcttc tcttgttgga
     7621 tgtggtttca tgtagaatgg cttgatggct tttggatagt ttaggataaa gacaggctgt
     7681 tcgaatgaat tcgcaatgaa cgtttcatga ggtgaaccaa agtcttcgcc ccattcgatg
     7741 tcatcaaatc catttttctt cagtaattcg attgcatcat cgtaagtaat acgagggaat
     7801 ggcaattttg tataagtttt taagacttct ttgtctcgtc ctagtacatc taatgcatat
     7861 tcgcaatgat ctaaaacatt ttgtactaag aatgctacat attgttcctg tacttctaaa
     7921 ctttcttctt gatgagtgaa agccatttct ggttcgatca tccagaattc gatcaaatga
     7981 cgtcgtgttt ttgatttttc agcacggaaa gtcggaccga atgaaaagac ttttccgaaa
     8041 gccatagcag cagcttccat gtataattgg ccactttgag ataaataagc tttttgatca
     8101 aagtaatttg tttcgaatag atccgtagtg ccttctggtg cagaaccagt caagatcggc
     8161 ggatcgattt tgacaaaatg atgttgatta aagaattcat acgttgcacg aatgatttca
     8221 tttctgatct gcataatcgc atgttgtctt gaagaacgca gccataaatg acgatgatcc
     8281 atcaagaaat ctgttccgtg ttcttttggt gtgatcggat aatcgtggct ctcgcttacc
     8341 acttccacat ttgtaattcc gatttcataa ccaaattttg aacgactgtc ttcacggatt
     8401 tctccagtta ctaacacgga tgtttcttgg tgtagatttt tagctgtttg gaaaacttct
     8461 tccgacactt cgctttttac aactacacct tggaaaaagg ctgttccgtc tcttaattgt
     8521 aaaaatgcga tttttccgct tgatcgctta tttgcgaccc aagcgccgat tgttactgtt
     8581 tctcccacat ggtttttcga atcgatgatt tgaatttgtt ccacgtatga ctctcctttt
     8641 atattccttg aatttcttca ggattaattt tgacttttac tctcaataaa ttgcttgatt
     8701 ctgcgtaccg cttcttctaa cgtagcgcga tcagtggcat aactcatgcg gacattttct
     8761 ggtgccccaa agccttcgcc agtcacaagc gctacccctg tttcttctaa aagatcttct
     8821 acccattgtg tgacattttc atacttacac atgcgtagcg tttcttttac attaggaaaa
     8881 agatagaaag ctccttgcgg cttttcaaga gaaacacccg gcagttctgc cacaagcgga
     8941 tataacgtat tcaggcgttc ttcaaaggcg ttccgcattt cttctactgt atcttgctca
     9001 cctttcaatg cttcaacagc tgcatattga ctgactgctg tcggattgct agttgattgg
     9061 gaagcaacag cgatcattcc attgatgatc acaggatttc ccaccgcata accaatgcgc
     9121 cagcctgtca tcgcatatgt tttagacacg ccgttgatga tgatggtctg atttttgatt
     9181 gcttctgaaa tcgttgcaat tggagtaaac tcattgccat tataaactaa acggccataa
     9241 atatcatcag caactatcaa aatatcattt tttactgccc attcaccgat tgcctgcagt
     9301 tcattcttgc tataaatcat tccagtcgga ttcgatggag aattgatgat tacggctttt
     9361 gttttttctg aacgtgcttg ttctagctgt tcaacagtaa ctttccagct attggattct
     9421 tcccctttca caaagacagg aagtccttct gccagtttta cttgctcacc gtaactgacc
     9481 cagtaaggca caggaatgat gacttcatct tgcgggtcaa ggatcgtttg gaaaagggta
     9541 tacaaagcaa atttcgctcc gtctgttacg actgtttgtg aaggttcata gacaagtcca
     9601 tagtttttct tcagataatc aacgattgcc tgtcgaagct ctgggattcc tgctgttggt
     9661 gtataatagc tagctttccc attgcggatt gcttcgattg ctgcttcttg gatgttttct
     9721 ggtgtcgcaa aatccggttc tcccacagtc aaactcagca catctctgcc ttttgctttt
     9781 aatgccttgg cctttgccgc agctgccaaa gtgacagacg gttctaatcg ttttgttctt
     9841 tttgataact ccatcccatt cattccttta ccttttaaat tcccttgatt gtcttgactt
     9901 catctccaga ttcaaagcca atcaggtaat aattcagttt cccatttgtt tcttttccta
     9961 ctacttccca cacaggctga tcatcataca tccctaaagc agctttttcg atttggattt
    10021 caggatgggc cgcagcaatt gtctgttttg cttcgttttc agtcagtccc tttgtttgat
    10081 ccaatacttt tactttttca ccagatttag gaatgataac ggcgatttct tttcctttat
    10141 catttgtacc tgtcagtgaa aagtatgtct tctttcgagt aaaccaataa aaatgatcgg
    10201 tttcctcaag atttgcatat ttctttgcga tttctactgc ttctttcttc gcctgcaccg
    10261 ccggacgact ggaacgcaag taaaaaatcg agctgatcac gatgaccgct aataaaaata
    10321 cgatcaagcc aagtaacacg cgattaatcg ttttttcctt tgtctgtttt ttcttcaagg
    10381 gtcgtcgctc ctttatcttc attccttttc attatagcag attttaatta tgaaaaaaga
    10441 cagctcctct aacttccttc ttcatttttc tttaagaatt catggatatc ctgatagatt
    10501 tcctccatag ctagctcttt gatcggtact tttttcggta atgcatggat gatccgtgcg
    10561 ccatattttg cagtgactag tcttcgatcc aaaataagca atacgccacg atctgtctcc
    10621 gtccttatca agcgacctaa tgcttggcgt aacttcaaag ctgctttagg tacggattcc
    10681 tggtaaaaag gattgatccc ctctgattct aaataagcgt tcctagcttt gaccatcggg
    10741 cgaagcggat tatcgaaggg taaacgtgtg actacaacga tctgcaaagc atctccaggc
    10801 aaatctacac cttcccaaaa gctgtcagcg ccaaacaata cgcttttttc tgacaaaatg
    10861 aagcgtttca aaagcttctc ccggcttcca ccaaaccctt gagctagtat ttctcgtcct
    10921 ttttctagca tagacagatg cattttctga tagaccttct gcaaaacatc atgagaagtg
    10981 aataacacaa gtattggccg atcttcttgt tcgatcagac ctgtaagtgc tgtctctaaa
    11041 taatcgatat atgcttgatt gctgacttga tggacggcag gtgcctcttt tggcacatag
    11101 atccttgcct gtttctcata atcataagga gaggcaatca ctttaagagg tgctgcttca
    11161 atgccaagcc tcttagagag atagtgtcga ttagggccga tcttcagcgt tcctccaata
    11221 taaagaatac gatcatatcg aggataccaa acagtcgacg gcaacatagc cgcttgaaaa
    11281 tcatggagat gaaaaatcat ctgctgcgct gtgttcccag gaacgaacca atggatcaaa
    11341 tgatctcgcc agtcttccag ccattgatcc atcagcaagt attgcttgtc taattgatca
    11401 aataggctat ataaacttgc taaatcgatc cgcatctggt ttgtccagct ttcttggata
    11461 atctcaaaag tcagacgcat ttgtgactgt agctgctgga tctcctgata aaaaagctgc
    11521 aggtgatcaa tggctgtccc agattccagt ggcagctgat ccaacatctc ttttgtcatc
    11581 atgacttctt ctggatactg tccgttaggt tgcttgccgc tcactaaact cattaatcct
    11641 tctgccatac aaatctgtgc ttcgcatagc gcagaaagtt cttctcgata aatcgataac
    11701 cagcgtaagg cttcatgttc tttcggtaaa tagctttgta ttcggtcaaa cagctgattt
    11761 ggttcttcta attgctggat ttgtttatgg aaatagttaa tacttaatcg tttgtcattt
    11821 actttttcag cgatttccgg caaatgatgt acttcatcga tcaacaaaaa tggactagct
    11881 ggaagcaatg gttcttttcg ctgtgtttct tgtactaaaa acgcatgatt gacaatcaaa
    11941 aaattacttt gcctgatttt cttttgcaag aaacgaacaa agtcttgttc gtaaaatgac
    12001 tgatttcttg ctaaaaatcc aatacctctg tgcgcgatat ctgcaaagaa tgaatggttc
    12061 aaatttgtta aatgtaattc atccaaatca ccagtcttcg tttgcgtcag ccaaactaag
    12121 accgccattt gatacaaagc atattgtttt tgttctggcg gttccacaag tgtgccttta
    12181 aaacgctgca aatcaatata gtggcgatag cttttgataa ttacaggttg tatgggctga
    12241 tctagcaatt gattcagcaa tggaatgtct ttttctatga tctgctgctg caaaactagc
    12301 gaaacggtac tgatgacagc gggtttttca ggtgtagcca aataactcaa tggcagcaga
    12361 taaccgattg ttttacccat tcctgtagct gcttcgatca tcaaatcttt tgattcatct
    12421 tttgtgaaat ggtcgtagac cgcattcatc agtctgttct gttcttttcg atacatcagt
    12481 gttttaccaa acattttttc ttttgcttgt ttttttcttg gatatgtccg ctcgccataa
    12541 tggacggatg tgaatagttc cacttctttt ttctgcaaag ctagcccatc gacgatttct
    12601 aatgagggat caagtggctc tggtttttct ttcatttctt caatcacatg ataaatgaaa
    12661 cgactcgtat ccatacctgt gtgtctactt aatcgcgcaa tctgctctaa cgtgatgatt
    12721 ggcaattttc tcatccgttc ttcgatcagc aaaagaagtt gacctgtcac ttcggcatca
    12781 ctatctgcct gatgaggatt ctcatgaata aatccaaggc tttctgacaa atcagctaag
    12841 cgaaagcttg gttccgtcgg caagaaaatt tgtgcaagtt ctactgtatc gatgcctggt
    12901 atcttcaaac taggaagtcc gcatcgcatg agttcatggt tcaaaaaatt ataatcaaaa
    12961 taaatattat gcgcgacaaa tatggtgtct gccaaaagat tgtaaatcgt ttgggcgaca
    13021 tcttcgaaat aaggcgcttt ttggacgcgt tgattcgtga tccctgttaa atgctggatt
    13081 tgttttgaaa tcttccgccc tggatgaata tcgatcgaaa aacgtgaagt gatccgacct
    13141 gattcaatca tgacgcagcc aaattggatg atccgatctt cttttggatt cgtcccagtc
    13201 gtttcgatat caacgactgc ataagttctt ggtttcaaaa tatcttcctc ttctcctgcc
    13261 tctaatgttc ttttgaaatt atactacaaa gtgcagaaaa atactctcat cgctttttaa
    13321 agaaaataga caaaaacgcc cagacacgca aatgtctggg cgtttatttt ttagtatagg
    13381 ttaattaagc tttagtaacg tttgttgctt gaggtccgcg ttggccttct tcaacatcga
    13441 aagttactgc ttggccttct tctaaagttt tgaatccgtc gccttggata gctgagaaat
    13501 gaacgaatac atcgtttccg ttttctgcag tgatgaatcc aaatcctttt tctgagttaa
    13561 accattttac tgtacctgtt tccataataa aaatcctcct tgtgtatcaa cacatatttt
    13621 gcaattactt gaatggtgta tatctggaag atgtttattt gaaacaagct accacagatt
    13681 accgaacaaa aactactcct atagtatatc ataccgttta caaaaaagct agagtttttc
    13741 gacttttttt attaaaaaaa gttttctcag cgattacata aagtgaaaaa ccgaactttt
    13801 ttttaaaata aacgacatat tgttcagttg gtgttttttt gctctgatgt tcttttttaa
    13861 actaaaaaaa ttattctttc tgactactta tcggttctgt ttcaacggtc gaggctgggg
    13921 aagattcttt agtaggtctg atgttctcat ggtatttctg atagcctatg acagttacca
    13981 gaagaacaag gcaaaccaat acgaccaacg agattagttt ttcttttttg ctcatatcta
    14041 tacctcataa ataacaggat gatgtccatc atatttctga gtatgagtaa aagcaaaagc
    14101 tgcagctgta gaatgtaaga aatgccagca actttcataa tcacaggtcg gacagtacca
    14161 aaagtaacca aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg cagaatcaag
    14221 atctggtgtc tgtacataaa catttttgta cagacgtgtg cctgtaatag gaaaccgctg
    14281 tgtttcttca gcaaaaactg gctgacttgt taaacttagg gatagagaca gcaaacacca
    14341 taacagtctt tttttcataa agacctcctt atcgataagt ttttcccaat taaaatcata
    14401 tctcaagaac gcccgttatt cacattttgt tcataaatga tgttttttgt atcataatcg
    14461 tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc agaagtagct
    14521 tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa taatttttgt caatctcttc
    14581 ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaaacggtg ttcaaaagct
    14641 tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa gagctatttt tgcttgctgt
    14701 tcttattact tgatgcagaa cagctttttc acaacctctc atacacggtg tttaatcagc
    14761 tgaccttcgg tattagctca aaaaacggca aaatatgtga aactattttt ccgtttgttt
    14821 ctctaatact caggtcaaaa cactgatttc acaacctctt ttaataacga agaatcgctg
    14881 tcagactctt ttcatctggc gcatcttttt gatccaaaat aaacactttt ccgccattaa
    14941 tgatcacatc atctgctaat gcattcaaca cttgacgccg atcatattct gtttcaggat
    15001 cttcaccgaa accatcagct aagtttgacg tagcgataaa taaatgtgac acttttcctt
    15061 ctttcgcagc tggaacgata tcaactaact gatcgatgaa tttcttatct aagagtttat
    15121 tgtattcttt tgattcttga gctgcgagtt cacttgacag cttcttcgcc ccttcttcga
    15181 tatctctaaa ggatagctgc gcaggagaag cagaaatcgc aacgtctgca caataatagg
    15241 gatttttagc aatttttttg aacatcgtct gattttctgg tagagcaaat aaataaatcg
    15301 gccattgttc tggattttcg aaagaatcct tcaagtacgt atcaactgct tggtaatagt
    15361 tgacccaatc gatctctact tcttcatctt ttggatttac gccatggaaa gttactccat
    15421 ctcgggctcc gcctcctcca ttagaagaga agttcaaacc gccacctgtc agttcagatc
    15481 ccaatgcagt aacgacatca accggtgccc cttctggtag agatacttct tttacttgtt
    15541 tgttgctgac ttcatacaat ttcatggagt cgcggttcaa tcccaataaa taataagaat
    15601 agttgaactg cccatttttg ataatagcca gtaaataagg aagatcactt acatagtatt
    15661 gatcgtctac cgggatactc aaacgatgga tataggtttg gtcagcagtt ataatgaccg
    15721 ctacactcgt agttgcacta cgccaaaacg aaggatctcc taacaaattt tcaatttttt
    15781 cttggaacgg ggaccagtca tgttcggaat attttttctc aaaacgtttc tttgcttctt
    15841 tcgcaaaatt tttgaatttg atctgatctt tttccacatc ttgatgggcg acatgcgtat
    15901 tcagcatgat cgtgacgaat ggtcctttag cgccttctga agtcaattgt gacaataatt
    15961 ctttatcctt ttttgccata aacgtacccc tcctaaaaaa atcattcctt tgtttctatc
    16021 taaagtctac cacaacacta tttttccata caataaaacg tatctaaaaa aataaaacgg
    16081 tttctacggt tttaaattta gctatttttt taagttatct tcataatttc ctcaattatt
    16141 tagccccagt cttcaactat tcttcatgaa atgctgataa actaagaaaa agaataaaag
    16201 aggtggatct tatgcatttg attatgattt tcagtttatt aagtattatc agtttatttg
    16261 ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc tattcctgta aaagtacgta
    16321 aaaacgaccg ctaaccttcc ataacgtgcg ttcttatcct aaagtttttt cagaaacatt
    16381 gaaactctct cccctctctg ataaaatttt aatttgtagc gaaacaaatt aattacaagg
    16441 agatcggata tgtccataaa aaaaacacgt ctagtcctat tattggctag tctcattctt
    16501 tgccttgctt cttttctacc agctgttgct gtgcaagctg aagatacttt caaagtaaat
    16561 gcaaaagcag cctttgctgt agatgcgcaa agcggtaaga ttctttatga tcaagatgga
    16621 gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg gtctttacat cgtccttgat
    16681 caagtaaaag aagggaaact atcatgggat gataaagttt caatttcaga ttatgcagaa
    16741 aatttgagtg tcaccccaga tttatcaaat gttcctttgc ataaagaaaa cacctacacc
    16801 gttaaagaat tattcgactc tgcaatcatc cagtcagcca acgcttcaat ggttgccttg
    16861 gcagaaaaaa tctctggcag cgaagcaaaa ttcactgaac gaatgaaaga acaattgaaa
    16921 gattggggta tcaaagatgc aacgatcgtt aatgcatcag gactgaataa ctcttatctt
    16981 ggcgaaaacc gaccagaagg tactggagaa aacgacgaaa atcaaatgtc tgctcaagat
    17041 gtggctatcg ttgcccgtca tttgatcttg gattttccag aaatactgga tgtatctagc
    17101 actacgacac aaatgtttgg cgaaaacacg caatcaccag tagaaatggt caactggaac
    17161 tggatgctcc caggattcgt gaattataaa gaaggcgtgg acggattaaa aactggtacg
    17221 actgaacttg caggagcctg tttcgtcggt acgatcacta aaaacgatca acgaatcatc
    17281 actgtcgtat tgaatgcaga cggccatgcg gaaaacccaa gtgcccgttt caatgaaaca
    17341 gctcgcttaa tggattattg ctacgacaat tggtcagaaa aagagcttgg aaaagccaat
    17401 gcaagtatcc ctagtctaaa aaccattgat gtaaaagacg gaaaagaatc aacagtgccc
    17461 gttgtgctta aaagtcctgt aaaagtatgg gtacggaacg atatggatac tggaaaatta
    17521 acaatcacac caactcttga caaaaaacaa gtcactgaca atgagatcca agcacctatg
    17581 gataaaggaa caaaaatcgg taccgcaacg atcaccttgg cagaagataa attaggttat
    17641 cttgaagaca acaacgcgcc atccacagaa atcatcacgg ataaaaccgt agaaaaagca
    17701 aatattttcg ttattggctg gcgacgcgtc gcagaatttt tcggtaactt gttttaattg
    17761 aacaaaaaga aaaaagcatc tttccatgtg gaagatgttt ttttgctttt tctagtttaa
    17821 ataacagctt ttttcatcta ttttcgttat actgtataag aaaaaggagt tgaatttatg
    17881 aatctaacca gagatctgat ttttttggtt ctctatctta ttggggtatt ctccatcgtt
    17941 tattggcgaa aacagaaaaa ggatccttcc ttgttctttt acagcagtac atggacgttt
    18001 gcggtaatct tgacttattt tgtcgtgttg gaaatttttt attcgacctt ttattttttg
    18061 attcccttag tgtttttcag tatctttgcc ttttcctatt tttcagaaaa gcgaaggctg
    18121 ttgaatggtc ttttattcaa tattttcctc attagttttg gtatctactt gtttgtcttg
    18181 ctatacgaaa cacaaaatat ttttcttggc ggactgattg ccttgatcac gatcccgtta
    18241 ctgcttgtct ttctattcgg catttatgga ttgatcgttt ttcttttttg gaacggtgtc
    18301 acagttttac gtagagagtc gcattcatta gctaatcttt taacgttgat actagccatt
    18361 tttcttacat tgtttcttgt ttttgacttt ttcttgttaa aatatttacc acaatggata
    18421 aatgctttgt ttttctgcat cccattgatc ttgatctatc tatttatcgt attctacaac
    18481 tttttgactg tctcattctt gtatcagttc aaccgccctc gatacaacca agacttcatc
    18541 gttgtgttag gcgccgggtt gatcaatgga gaaacagttt ctcctcttct tgccaagcga
    18601 atcaataagg cgattgcttt ttattgggca caaagccgtg cgacattaaa tccgcctatc
    18661 ctattaatgt cgggtggaca gggagcagac gaaaaagtac cagaagcaat ggcgatgaaa
    18721 caatatgcca tggaacaagg aattcctgaa agagatattc ttgtcgaaac caattcgaca
    18781 accactttgg agaatatgcg ctattcaaag gaaatcatgg atcagcaaat gaaaggtccc
    18841 tatcgcgcga tcttcagttc caacaattac cacattttca gagctggtct ctatgcacga
    18901 caagcgaaac tgaaggcaaa tggcattggg gcaaaaacag ctttctatta cttgcctaac
    18961 gcgtttttaa gagaatacat cgccatcttg atgatgaaca aaaaaagaca tatgatcatt
    19021 tgcggattgc ttttcgcagg ttccgcattc ttatcactga tcactttact tttctaaaaa
    19081 aacaaggtgt gacaaaacat atgtcacacc ttgttttttt ctttcattct cttaactatt
    19141 ggcaaaaaga gtatcccttt tctgaccgct acttgctgag cagaataaat accgctgttg
    19201 cctgaacaca taaagctgat caagcagatc ataaagaagt atactgccgc ttgtgaaccg
    19261 aacaattcga tccccatcac aaagcaggca atcggtgtat ttgtcgcacc agaaaatact
    19321 ccgataaagc ccaatcctgc aagaaaagga acagaaacat ggagcaaagg cgctagtgct
    19381 gcaccaagcg ttgcaccaat ttcaaacaat ggtgtcactt ctcccccttg atatcccgcg
    19441 cctaaagaca aaactgtgaa gaataacttc ccaataaaat catagacatg tgcctgtcca
    19501 ttaaatgatt cctgcagtaa tggaagactc aatccgagat aacgttgtgt ttgcaaaatc
    19561 aatgctgcgg ctacaactac agccccacct atcgtatttc gccaaacagg atgaggaatc
    19621 cattgtgcat atcgcgcttt taaaaagacg atcgaacggc tgaataccca accaatcaaa
    19681 ccaaaagcaa tcccagcaac aaaaactttg aagaacagca acatactcca ttcaggaatc
    19741 acacccattt ggtaatgtag atgagaaaca ccatagcttt ctgtcataaa atttgcaaac
    19801 agtgcagcaa agaagctagg gaagatcgct tctgttcgga ctttcccgat tgccaacact
    19861 tctaatccaa ataatgttcc agctaaaggt gtgccaaata cagaactaaa acccgcactg
    19921 attccactga taatcaagat ttcccgttct gatttatcca atcggaacaa acgtgctgta
    19981 ttatctgcta acgctccccc catttggact gctgttcctt cacggccaac agatcctcca
    20041 aataaatggg tagttatggt tccaaacagt gtaagtggaa taagtcgcaa cgggattttt
    20101 tcttctccac catttccttg atcgatcact aagttgtttc cacgactaga aagaccgccg
    20161 tgatacgcat agagataagc aaaaagcgca ccactaactg gtagtagata aaggagccaa
    20221 ggatgtgata gtcggatatc tgtcacaact gtcaggctat ttaagaaaaa agcagataag
    20281 ctccccatga atataccgag aatcgctgtg atcaccagcc acttcagcat gtacactaaa
    20341 atgcgtactg gactcggtaa ttgtttttcc ataaaaaatt cctcctcaat aaaaaaaggc
    20401 tctataattt atgggactga tgaatcagaa ttgattcttc agccccattt tcattttagg
    20461 tattaaaaaa catatcgttc t
//
LOCUS       DAZUMT010000051        20181 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000051 DAZUMT010000000
VERSION     DAZUMT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.051"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACG8FM_001641"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACG8FM_001641"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS5094304.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACG8FM_001642"
     CDS             1011..2258
                     /locus_tag="ACG8FM_001642"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS5094305.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACG8FM_001643"
     CDS             2334..2678
                     /locus_tag="ACG8FM_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094306.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACG8FM_001644"
     CDS             complement(2795..3121)
                     /locus_tag="ACG8FM_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094307.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="ACG8FM_001645"
     CDS             complement(3494..4696)
                     /locus_tag="ACG8FM_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5094308.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="ACG8FM_001646"
     CDS             4813..5328
                     /locus_tag="ACG8FM_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5094309.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="ACG8FM_001647"
     CDS             complement(5527..5973)
                     /locus_tag="ACG8FM_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5094310.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACG8FM_001648"
     CDS             complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACG8FM_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5094311.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="ACG8FM_001649"
     CDS             complement(7852..8481)
                     /locus_tag="ACG8FM_001649"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5094312.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="ACG8FM_001650"
     CDS             complement(8465..8929)
                     /locus_tag="ACG8FM_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5094313.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="ACG8FM_001651"
     CDS             complement(9416..10684)
                     /locus_tag="ACG8FM_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5094314.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="ACG8FM_001652"
     CDS             complement(10915..11652)
                     /locus_tag="ACG8FM_001652"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5094315.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="ACG8FM_001653"
     CDS             complement(11719..12288)
                     /locus_tag="ACG8FM_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS5094316.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="ACG8FM_001654"
     CDS             12416..13288
                     /locus_tag="ACG8FM_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5094317.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="ACG8FM_001655"
     CDS             13630..14502
                     /locus_tag="ACG8FM_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5094318.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /gene="nagE"
                     /locus_tag="ACG8FM_001656"
     CDS             14499..16457
                     /gene="nagE"
                     /locus_tag="ACG8FM_001656"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS5094319.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="ACG8FM_001657"
     CDS             complement(16708..17559)
                     /locus_tag="ACG8FM_001657"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5094320.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="ACG8FM_001658"
     CDS             17682..18149
                     /locus_tag="ACG8FM_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS5094321.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="ACG8FM_001659"
     CDS             complement(18412..18894)
                     /locus_tag="ACG8FM_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5094322.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="ACG8FM_001660"
     CDS             complement(18994..19857)
                     /locus_tag="ACG8FM_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5094323.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6067 a         4156 c         3662 g         6296 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgacta caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgactccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactaacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt t
//
LOCUS       DAZUMT010000052        20024 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000052 DAZUMT010000000
VERSION     DAZUMT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.052"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..289)
                     /locus_tag="ACG8FM_001661"
     CDS             complement(32..289)
                     /locus_tag="ACG8FM_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094324.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            451..1560
                     /gene="pdhA"
                     /locus_tag="ACG8FM_001662"
     CDS             451..1560
                     /gene="pdhA"
                     /locus_tag="ACG8FM_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS5094325.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1563..2540
                     /locus_tag="ACG8FM_001663"
     CDS             1563..2540
                     /locus_tag="ACG8FM_001663"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS5094326.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2565..4208
                     /locus_tag="ACG8FM_001664"
     CDS             2565..4208
                     /locus_tag="ACG8FM_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS5094327.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4216..5622
                     /gene="lpdA"
                     /locus_tag="ACG8FM_001665"
     CDS             4216..5622
                     /gene="lpdA"
                     /locus_tag="ACG8FM_001665"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS5094328.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5784..6059
                     /locus_tag="ACG8FM_001666"
     CDS             5784..6059
                     /locus_tag="ACG8FM_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS5094329.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            6065..6832
                     /locus_tag="ACG8FM_001667"
     CDS             6065..6832
                     /locus_tag="ACG8FM_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS5094330.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            7000..8829
                     /gene="typA"
                     /locus_tag="ACG8FM_001668"
     CDS             7000..8829
                     /gene="typA"
                     /locus_tag="ACG8FM_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS5094331.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            9090..10862
                     /gene="recQ"
                     /locus_tag="ACG8FM_001669"
     CDS             9090..10862
                     /gene="recQ"
                     /locus_tag="ACG8FM_001669"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS5094332.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10976..11278
                     /locus_tag="ACG8FM_001670"
     CDS             10976..11278
                     /locus_tag="ACG8FM_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS5094333.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11281..12444
                     /locus_tag="ACG8FM_001671"
     CDS             11281..12444
                     /locus_tag="ACG8FM_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5094334.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12470..15898
                     /locus_tag="ACG8FM_001672"
     CDS             12470..15898
                     /locus_tag="ACG8FM_001672"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS5094335.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15919..17043
                     /locus_tag="ACG8FM_001673"
     CDS             15919..17043
                     /locus_tag="ACG8FM_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS5094336.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            17024..17470
                     /locus_tag="ACG8FM_001674"
     CDS             17024..17470
                     /locus_tag="ACG8FM_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5094337.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17454..17792
                     /locus_tag="ACG8FM_001675"
     CDS             17454..17792
                     /locus_tag="ACG8FM_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS5094338.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17911..18471
                     /gene="rsmD"
                     /locus_tag="ACG8FM_001676"
     CDS             17911..18471
                     /gene="rsmD"
                     /locus_tag="ACG8FM_001676"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS5094339.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18468..18959
                     /gene="coaD"
                     /locus_tag="ACG8FM_001677"
     CDS             18468..18959
                     /gene="coaD"
                     /locus_tag="ACG8FM_001677"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS5094340.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18949..19992
                     /locus_tag="ACG8FM_001678"
     CDS             18949..19992
                     /locus_tag="ACG8FM_001678"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS5094341.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         6786 a         3494 c         4402 g         5342 t
ORIGIN      
        1 taaatagatt aaagaatata acagcttgga tttaaatttc aagctgtttc ttttttttcg
       61 gaaaaagaat acgatataaa gaaactaaga aaaaaccatt atttgtacgt ccgttcacga
      121 tgtgtaaata aaataaatgt ttgcaacaaa actgtataac accaacgttt ttaaatagaa
      181 acagatggtt gtaagtttga ttagaaaata gaaaaaatgc agatgaaata taaaacgctt
      241 tccttattgt gattttattc actaatgctg ttaatatgcg gattggcata atttcctttc
      301 atcgtgaaaa tttgtgcttt tagcttgtat ttctcataaa tccatgataa tttattaatg
      361 aagacggtta aatagtgata cagttttatt cactgtttca atcgcagaat gaagagattt
      421 tcttatagat gagagaggaa tgtgtgacaa atggcaaaca aaaaagcacc gatcgacttc
      481 caagcactgc tagatgaagt caatgcagat ttcccagtat atcaagcact tgatcaagat
      541 ggaaacgtag ttaacaaaga tttagtacct gatttatccg atgatgaact cgttgaatta
      601 atgactcata tggtatggtc tcgtgtatta gaccaacgtt caacagcgct gaaccgccaa
      661 ggacgcctag gcttttttgc accaactgct ggacaagagg caagtcagct ggctagtgct
      721 tacgcatttg ataaagaaga tgttcttctt ccaggttatc gggatgtacc tcaactagta
      781 aaacatggtt tacctttatc tcaagctttt ttatggtctc gaggacatgc tgcaggtaat
      841 tcttatccgg aagaattgaa agctctacca ccacaaatta tcatcggtgc acaatatgta
      901 caagcagccg gtgttgcatt aggtttgaag aaacgaaaca agaaaaatgt cgtcttcact
      961 tatactggtg atggcggttc atcacaaggt gatttttacg aagcaatcaa tttcgctggc
     1021 gcttaccatg caaatgccgt attttatatc caaaacaacg ggtatgcgat ctctacacct
     1081 cgtgaaaaac aaactgcagc aaaaacactt gcacaaaaag ctgtcgcagc tgggattcct
     1141 ggtattcaag tcgatggaat ggatccgctt gcagtatata ctgtatctaa aatggcacgg
     1201 gaatgggcga tcagtggaaa tggaccagtc ctaatcgaaa cactgactta tcgttatgga
     1261 ccacatacac tatctggtga cgatccaacc cgctaccgta caaaagacac agatgacgat
     1321 tggcagaaaa aagatccatt gatccgcttc cgtaaatatt tgacggaaaa aggactttgg
     1381 tctgaagaaa aagaagaaca agtcatcgaa gcaactaaag aagaaatcaa agcagctatt
     1441 gctgaagccg acaaagtacc aaaacaaaag gtttcagatt tcttgaaaaa catgttcgaa
     1501 gtatctccac aatcaatcaa agaacaaatt gcaatttatg aagcgaagga gtcgaaataa
     1561 tcatggcaca aaaaacaatg atccaagcaa tcacagatgc cttagcactt gaactagaaa
     1621 atgacgagaa tgtcgttgtt tttggtgaag atgttgggaa aaacggagga gttttccggg
     1681 caactgaagg attgcaggaa aagttcggag aagaccgagt attcgatact cctttagctg
     1741 aatctggaat cgcaggattg agtttcggtc ttgcgttaga aggatttcgt ccagttcctg
     1801 aaatccaatt ctttggtttt attttcgaag caatggacga agtagtagct caaatggctc
     1861 gtacaagata tcgaatgagc ggcactagaa atctacccat cacaattcgt tcgccatttg
     1921 gtggcggtgt tcatacacca gaattgcatt cggataattt agaaggtttg atcgctcagt
     1981 ctcctggtat tcgagtagtg attccttcca acccttatga tgcaaaagga ctattgattt
     2041 cttctattag aagtaacgat ccggttgttt tcctagaaca catgaaacta tatcgctcat
     2101 tccgagaaga agtgccggat gaagcttacg aagttccttt ggataaagca gcagttactc
     2161 gtgagggaac agatgtatct attatcacat acggagcaat ggtgcgtgaa gcaatcaaag
     2221 cagcagacaa tcttgcaaaa gaaaatatat ctgttgaaat cattgatctt cgtacagttg
     2281 cgccattaga tgtagaaacg atcattcaat ccgtggaaaa aactggtcgc gtagttgttg
     2341 tccaagaagc acaacgacaa gcgggcgttg ccgcgcaagt agtttctgag atctcagaac
     2401 gcgctatcct ttcattagaa gccccaatcg gacgagtttc tgcacctgat acagtcttcc
     2461 catttggaca agcagaaaat gtctggttgc caaatgcaaa agatatcgaa gacaaagtaa
     2521 aagaaatcgc ggaattttaa gaaaagaagg gacgaaacaa agtcatggct tatcaattta
     2581 aattgccgga tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac
     2641 caggagatac gatcaatgaa gatgatacat tactagaagt tcaaaatgac aaatcagtag
     2701 aagaaatccc gtcacctgtt acaggaacgg ttaaaaatgt gattgttcca gaaggtaccg
     2761 tagcaaatgt tggggatgta ttagtagaaa tcgatgcacc aggacacgaa gacaatgaag
     2821 gggacagtgg tgtagccgca gaatcccaaa ctccagctaa accggctgct gaacccactg
     2881 ttgatacaga atcagctgga tcgtcaagcg aaggagtctt ccaatttaag ttaccggata
     2941 tcggtgaagg aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga
     3001 tcaatgaaga tgatacatta ctagaagttc aaaatgacaa atcagtagaa gaaattccgt
     3061 cacctgtcac agggacagtt aaaaacgtga ttgttccaga aggcactgta gcaaatgttg
     3121 gggatgtatt agtagaaatc gatgctcctg gacacaatag tgcaccgtca actagtgcgc
     3181 catcagctga agcaccaaaa gaaaaagtgg aaacatcagg ttcagcaagt gtcgttgaag
     3241 ctgccgatcc aaataaacgt gtattggcta tgccatctgt tcgtcaattt gcccgtgaaa
     3301 aagatgtaga tatctcacaa gtaactgcaa ctggaaaagg tggcagagta acaaaagagg
     3361 acatcgaaaa cttcttggca ggtggtccaa gcagtgcacc agcgaaatca gaagcgccag
     3421 aagcggcagc accgaaagaa gcagctcctg cagcagaaag caaaccagct gcaccagcaa
     3481 aaccattcaa gtccaactta ggagacttgg aagaacgtgt agcaatgacg ccaacacgta
     3541 aagctatcgc taaagcaatg gtcaacagta agcatacagc tccacacgtg acattgcatg
     3601 atgaagtaga agtgtctaaa ctatgggata atcggaaacg atttaaagaa gttgctgctg
     3661 caaatggtac gaaacttact ttcttgccat atgtcgtgaa agctttaaca gcgactgtta
     3721 agaaataccc agtattgaat gcatctatcg acgatgcaaa ccaagaaatc gtttataaac
     3781 attactacaa tatcggtatt gcaactgata cggatcacgg actatatgta ccaaatgtga
     3841 aagatgcaga ccgcaaagga atgtttgcta ttgcagacga aatcaatgag aaagcaaaat
     3901 tagctcatga cggcaaacta tctgcagaag atatgcgcaa tggaacaatt acgatcagca
     3961 atatcggatc tgtaggtggc ggttggttta caccagtcat caactatcca gaagtagcta
     4021 ttttaggtgt aggaacgatt gcacaacaac caatcgtaaa tgctgaaggc gaaatcgttg
     4081 tcggtcgtgt gatgaaactg tcattaagtt tcgatcaccg tatcgtagat ggagcaactg
     4141 cgcaacaagc aatgaataat atcaaacgtc ttttagctga tccagagcta ttaatgatgg
     4201 aaggatgata aacaaatggt agttggagat tttgctgtag aattagatac agttgtaatt
     4261 ggtgcgggac ctggtggcta tgtagctgcc atccgcgctg ctgaaatggg acaaaaagta
     4321 gcaatcattg aaagagagta tattggcggt gtttgtttga atgtcggctg tattccttca
     4381 aaagcactga tagctgcagg acaccattat caagagtcac tagattctga aatgtttgga
     4441 gtaactagtg aaaacgtcaa actagatttt gcaaaaacac aagaatggaa agaaaacaaa
     4501 gttgtccata cgttgacttc tggtgttggt ttcctattaa aaaaacataa ggttgaaaca
     4561 atcgaaggag aagcgttctt cgtagatgat cacacactac gcgtcattca tccagattca
     4621 gctcaaactt attcgttcaa taacgcaatc atcgcaacag gttcccgtcc aatcgagatt
     4681 ccaggattta aattcggagg acgtgtgtta gattctactg gcggattagc attgaaagaa
     4741 gtacctaaga aatttgtgat catcggtggc ggtgtgattg gtgccgaatt gggcggagcg
     4801 tacgcaaact taggagcaga agtaacgatt cttgaaggct caccacaaat tttaccaact
     4861 tacgaaaaag atctagtaaa attagtagaa gatgatttca agaaaaaagg cgtaacagtc
     4921 gtaacgaatg caatggcgaa agaagcagtc gataacggtg acagcgtgac agtgaaatat
     4981 gcagtggacg ggaaagaaga atccgttact gctgattatg tgatggtcac tgtaggtcgt
     5041 cgtccgaata cagatgatat gggcttagaa caagcgggtg tcgaagtagg agaacgagga
     5101 ttgatcacgg tggataaaca aggacgtacg aacgtaccta acatctatgc catcggtgat
     5161 atcgttccag gggcggcatt agcacacaaa gccagttatg aagcaaaaat cgctgctgaa
     5221 gcaatctctg gcaaaaaagt agctgtagat tataaagcaa tgccggcagt agccttcact
     5281 gatcctgaat tggcttctgt aggtatgacg atcaaagaag caaaagatgc tggtatcgaa
     5341 gcaaaagcat ataaattccc attttcaggg aacggtcggg cattatcgct tggtaaaaca
     5401 gaaggattca tccgtctagt tacgacgatc gaagataacg tgcttattgg cgcgcaaatc
     5461 ggtggagtcg gcgcaagtga tatggtctct gaattagctt tagcaatcga atcaggaatg
     5521 aatgctgaag atatcgcctt gacgattcat cctcatccat ctcttggcga aatcgtcatg
     5581 gatgcttcgg aattagcatt aggtttacca atccatattt gatttgaata atacaggcga
     5641 aacgcaactg aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt
     5701 aaacttaaaa atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg
     5761 aatatgaaat aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga
     5821 caacagacga aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa
     5881 aagggataaa caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat
     5941 ccattggtga ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat
     6001 atcgtacagt gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag
     6061 ataaatggaa cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat
     6121 caaatcttct caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac
     6181 aaatgtcgat cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga
     6241 ggcgaaaata ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt
     6301 ctttatcatc gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg
     6361 tattatgctc gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat
     6421 gagaaatgaa cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt
     6481 gcctagacca gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt
     6541 cggtcataat aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta
     6601 cggatgcgct ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc
     6661 aaaccttagc ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt
     6721 aacaagcggt ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg
     6781 gacttcttgt gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg
     6841 tagaaatcat tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga
     6901 aaacatttta ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg
     6961 ccctgtcata aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc
     7021 cgtaacgtcg caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta
     7081 ttaaaacaat cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca
     7141 aatgcgctag aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat
     7201 aaaggtattc gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta
     7261 gaacggatca tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca
     7321 atgcctcaaa cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt
     7381 gtcgtaaaca agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt
     7441 gaactattca tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca
     7501 tctgcactaa atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg
     7561 gcacctattt ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa
     7621 ccattacaat tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc
     7681 ggacgtgtat ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac
     7741 ggaacagtta agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta
     7801 gagattcaag aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt
     7861 gtcggtgaaa cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac
     7921 gaaccaacat tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt
     7981 aaattcgtaa ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta
     8041 tccttacgag ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa
     8101 ttgcatttgt ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct
     8161 cgtccagaag ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg
     8221 caaatcgata caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa
     8281 ggtgaaatgc aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca
     8341 cctgcccgcg gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga
     8401 atcatgaacc atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt
     8461 caccaagggg cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt
     8521 atcgaagaac gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt
     8581 atcggggaaa acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg
     8641 acaaacgtac ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg
     8701 acgttggaag aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa
     8761 agcattcgct tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag
     8821 aaaaaataat ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt
     8881 tctttttttg tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca
     8941 gaaaatacgc cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg
     9001 tttcataata agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa
     9061 gtaaaatggc aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct
     9121 atgatgagtt tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg
     9181 tattaggaat catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac
     9241 tattagatgg gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg
     9301 acgcggcgaa tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg
     9361 aaacagctag acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc
     9421 ctgaacgttt tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga
     9481 tcgctattga cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt
     9541 tacagatggc gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag
     9601 ctacagcgac tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca
     9661 accatattca gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa
     9721 aaaaagaaca atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt
     9781 atgcagcaac gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt
     9841 ccgtgggtag atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct
     9901 ttttatacga tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata
     9961 aaagcaatgt ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc
    10021 aggaagctgg tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc
    10081 ctcaagatat ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa
    10141 agcaaaaaga atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc
    10201 ttcagcaata catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta
    10261 actgtttaga tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt
    10321 gcttaaaacg tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat
    10381 cgaaagaaca aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga
    10441 aaaaccaatc gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc
    10501 ttctgaccat caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat
    10561 tgaaaggaca agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg
    10621 aagaaaccga tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag
    10681 caggcgttcc cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac
    10741 gtccatcaag cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata
    10801 aatatggcga agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt
    10861 agaaagaacg aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc
    10921 cttatgaaaa tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga
    10981 ttctttgtcg aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg
    11041 attaatccga aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt
    11101 ggtagataca cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt
    11161 aatcggaaag aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt
    11221 atatagtgag gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac
    11281 ttgcctaaga aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta
    11341 attttatgtg tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa
    11401 aacggacaaa atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg
    11461 attgctatcg cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc
    11521 atggcagcca tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag
    11581 atcaatgggg cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac
    11641 ctaaaaatca tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta
    11701 caaaaagatt ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca
    11761 ttagtcgcta ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt
    11821 ctgcttttag ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt
    11881 tgtctaagta cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga
    11941 cgtttacagt atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta
    12001 aataaagggc atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc
    12061 aggggattag gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt
    12121 atttatgcaa tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg
    12181 ctattcttta tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat
    12241 tcgcttttgt gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga
    12301 ggaattactg gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc
    12361 tccagtttgc tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa
    12421 aaacgcaaga gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt
    12481 tttagttgct aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg
    12541 aattaaaact gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc
    12601 ggatgaagca tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga
    12661 agggatcatt tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct
    12721 tttgtcggaa aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg
    12781 acccaaactg catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat
    12841 cgaagcgggg attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc
    12901 cttggagttt gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg
    12961 cggacgcggc atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc
    13021 gaaaagcgaa gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc
    13081 taatcctaag catatcgaag tacagattct cggggatacg catggaaacg tcatccatct
    13141 ttttgaaaga gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg
    13201 tgtctctatg aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa
    13261 atatgtcggg tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta
    13321 ctttatcgaa gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga
    13381 tattgatatc gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat
    13441 cggattgcct cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac
    13501 tacagaagac ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc
    13561 acctggtggt tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac
    13621 accgtatttt gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc
    13681 cattcagaaa atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat
    13741 tccatttatg ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac
    13801 cttcattgat agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa
    13861 aacgatgaaa tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga
    13921 aaaaccattt tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt
    13981 aacggctaag aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg
    14041 acaggaaaat ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc
    14101 aactcgtgtt cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc
    14161 tgaactcttt tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt
    14221 gaatgaagat ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct
    14281 gcaaatgctt ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt
    14341 ggtggaattt gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag
    14401 tttgaactgg actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat
    14461 cgcagaggca gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa
    14521 tgttcagtat tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc
    14581 aatcaaagat atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt
    14641 gaaagaaacg acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat
    14701 catcacttat tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc
    14761 aatgagtggg aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg
    14821 tccacggctg ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga
    14881 tgtgcgcatg tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata
    14941 tatgcatgaa atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg
    15001 attaggtcat cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg
    15061 cgatatcgtt aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt
    15121 ccaaaatgat cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga
    15181 atctgttgtg actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa
    15241 attgcaaaag attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc
    15301 tgaatcagtc gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc
    15361 aaaacaagac gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa
    15421 agcatataat caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat
    15481 acgtttgggt gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt
    15541 agatgaaatc ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg
    15601 acaaagacgt gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt
    15661 gaaagctgaa ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga
    15721 tgtattagtg aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc
    15781 gatgaaaatg gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt
    15841 gtcttcagga gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata
    15901 agcgaaaggt ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt
    15961 attgtggcat tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac
    16021 tctgatcaac aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact
    16081 ggtttttcta cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc
    16141 tatgatcgat taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt
    16201 catctcacat ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa
    16261 acagggcctg ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa
    16321 atcactacta tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa
    16381 ctaatggaag aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct
    16441 attttatttt ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat
    16501 agtcttttga aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca
    16561 gataaatcgg ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta
    16621 ttaaatatgc ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa
    16681 aaccacagtc agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag
    16741 gaacgtatgg ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca
    16801 ctgacgaatg atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg
    16861 ctatttattc atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct
    16921 tacaaccatg accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa
    16981 gaaaatatgt tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt
    17041 taacgaagag ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa
    17101 gagtaaaacg atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt
    17161 ttgccagttg aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata
    17221 tggtgagtat gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca
    17281 gcttgatatg gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct
    17341 gttggatcaa attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc
    17401 aggtagcccg ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat
    17461 tagacaatag tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct
    17521 taaaacaatt aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga
    17581 aatatgtcgt gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta
    17641 aattacattt tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg
    17701 ccgaaaaaat aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa
    17761 atacagaaat ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa
    17821 aaaataactt ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta
    17881 gtaccagtac catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg
    17941 cgccgtttaa aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa
    18001 tccatattca atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca
    18061 ggaagcggtg gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta
    18121 gacaaaaatt accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca
    18181 gagaaattta ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag
    18241 aaacaacaat tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat
    18301 caaatccata aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa
    18361 acggataaaa cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag
    18421 atttatggca taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat
    18481 tattcccagg aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg
    18541 ctaaattatt cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt
    18601 ttcccccaga agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa
    18661 aagtcatgca tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg
    18721 cgttgatcag aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga
    18781 tgaatcatca tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata
    18841 gtcacgtgag ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct
    18901 acttaccgcc agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa
    18961 caatggttga aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta
    19021 gttcccttgc cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt
    19081 acggtcaatg ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc
    19141 aggcaagcta caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc
    19201 agtgaagaag aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat
    19261 tatatggatt cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct
    19321 tataaaatgg aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt
    19381 ggcaagcttt cagttggaga tacagtaaca aaagtagacg ggaaaagctt tcaaagtaca
    19441 gaagcattta tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat
    19501 ttgcataatg gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa
    19561 aaggcaggaa taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt
    19621 cgtattaatt ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc
    19681 tatgaacaag tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg
    19741 atcaacagtg aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg
    19801 gataaagctg gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag
    19861 aaagaatatc ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg
    19921 aattcctcaa tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag
    19981 aaaatgaaat gaaactaaat aattaaagag gggttgtgac acga
//
LOCUS       DAZUMT010000053        19507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000053 DAZUMT010000000
VERSION     DAZUMT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.053"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8FM_001679"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8FM_001679"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS5094342.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACG8FM_001680"
     CDS             complement(1173..1868)
                     /locus_tag="ACG8FM_001680"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS5094343.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACG8FM_001681"
     CDS             complement(1881..2597)
                     /locus_tag="ACG8FM_001681"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS5094344.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACG8FM_001682"
     CDS             2927..3532
                     /locus_tag="ACG8FM_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS5094345.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACG8FM_001683"
     CDS             3857..4180
                     /locus_tag="ACG8FM_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS5094346.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACG8FM_001684"
     CDS             4362..5732
                     /locus_tag="ACG8FM_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS5094347.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACG8FM_001685"
     CDS             complement(5862..7163)
                     /locus_tag="ACG8FM_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5094348.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACG8FM_001686"
     CDS             complement(7393..9384)
                     /locus_tag="ACG8FM_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS5094349.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDKVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACG8FM_001687"
     CDS             complement(9528..9794)
                     /locus_tag="ACG8FM_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5094350.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8FM_001688"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8FM_001688"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS5094351.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8FM_001689"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8FM_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS5094352.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACG8FM_001690"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACG8FM_001690"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5094353.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACG8FM_001691"
     CDS             complement(13954..14475)
                     /locus_tag="ACG8FM_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS5094354.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACG8FM_001692"
     CDS             complement(14478..15320)
                     /locus_tag="ACG8FM_001692"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS5094355.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACG8FM_001693"
     CDS             complement(15313..16038)
                     /locus_tag="ACG8FM_001693"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS5094356.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACG8FM_001694"
     CDS             16214..16510
                     /locus_tag="ACG8FM_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094357.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACG8FM_001695"
     CDS             17078..18670
                     /locus_tag="ACG8FM_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5094358.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACG8FM_001696"
     CDS             18683..19423
                     /locus_tag="ACG8FM_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5094359.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4001 c         3702 g         6224 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt tatcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAZUMT010000054        19410 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000054 DAZUMT010000000
VERSION     DAZUMT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.054"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..3562
                     /locus_tag="ACG8FM_001697"
     CDS             35..3562
                     /locus_tag="ACG8FM_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS5094360.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3559..7281
                     /gene="addA"
                     /locus_tag="ACG8FM_001698"
     CDS             3559..7281
                     /gene="addA"
                     /locus_tag="ACG8FM_001698"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS5094361.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7350..7691
                     /locus_tag="ACG8FM_001699"
     CDS             7350..7691
                     /locus_tag="ACG8FM_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5094362.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8000..9043
                     /gene="pheS"
                     /locus_tag="ACG8FM_001700"
     CDS             8000..9043
                     /gene="pheS"
                     /locus_tag="ACG8FM_001700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS5094363.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9048..11468
                     /gene="pheT"
                     /locus_tag="ACG8FM_001701"
     CDS             9048..11468
                     /gene="pheT"
                     /locus_tag="ACG8FM_001701"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS5094364.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11638..12648)
                     /gene="trpS"
                     /locus_tag="ACG8FM_001702"
     CDS             complement(11638..12648)
                     /gene="trpS"
                     /locus_tag="ACG8FM_001702"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS5094365.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12902..13639
                     /locus_tag="ACG8FM_001703"
     CDS             12902..13639
                     /locus_tag="ACG8FM_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5094366.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13653..14468
                     /locus_tag="ACG8FM_001704"
     CDS             13653..14468
                     /locus_tag="ACG8FM_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5094367.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14481..15128
                     /locus_tag="ACG8FM_001705"
     CDS             14481..15128
                     /locus_tag="ACG8FM_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5094368.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15144..15800
                     /locus_tag="ACG8FM_001706"
     CDS             15144..15800
                     /locus_tag="ACG8FM_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5094369.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            15962..16783
                     /gene="racE"
                     /locus_tag="ACG8FM_001707"
     CDS             15962..16783
                     /gene="racE"
                     /locus_tag="ACG8FM_001707"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS5094370.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16786..18141
                     /gene="rph"
                     /locus_tag="ACG8FM_001708"
     CDS             16786..18141
                     /gene="rph"
                     /locus_tag="ACG8FM_001708"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS5094371.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18142..18660
                     /locus_tag="ACG8FM_001709"
     CDS             18142..18660
                     /locus_tag="ACG8FM_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5094372.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18767..19267
                     /gene="cbpB"
                     /locus_tag="ACG8FM_001710"
     CDS             18767..19267
                     /gene="cbpB"
                     /locus_tag="ACG8FM_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS5094373.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6518 a         3341 c         4172 g         5379 t
ORIGIN      
        1 ggagcaccgt atcatgtgtt gaaggaggaa aagcatgtct ttacaattta ttcttggaaa
       61 cgggaaaaaa gatcaccgtc aagtcttgat cgacgaagca tccgcttggc tcgaccagga
      121 tgcaagtaac caagtatttt tccttgttcc caactacagc aaattcgaac aggaacaaga
      181 aattttagcg gagatgcgaa gaagaagcgg aaaaaaaact ttcagcacaa caaacgttca
      241 agtatttagt ttttatcgtc tggcttggta tttgctgcaa cagacaactc ttcttacagg
      301 aaatgaatta tcagaatcag gatctgcaat gatcttgcga cagattttag aaaaaaatga
      361 ggaagaatta acaatctttc gcggagaaat caataaaaga gggtttattc gtcaattaca
      421 agaactctac agtgaattgc gtacaggaaa tattcaacca gaagacctga tgcttttatt
      481 tggtgattca cctaatgcag aagaccagca acttaaaatg aaagacttga agctgatttt
      541 ttcagcttat gacagcgaat tgactcaacg tgcactaaca aatgaagatg cactatctat
      601 actagcaaat tatatgcaga cacaagattt caacaacgtc aaatttattg tcagtggttt
      661 ttctcgaatc aatggtcaag agtatcaatt actgcaggca atgatggaaa aggggcaatt
      721 ggttgtcgat ttattgctgg atcgtcctta tacagcggat cttccagaag tacttacgct
      781 ctttcatgag actggaaagc tttatttccg tctttttcaa agtgcaagag aacggaaagt
      841 ccctatatta gtggaccgat ttgcaccaga gcagactcgt ccgacagagc ttcaactgct
      901 gcaaacaatg tgggaagaga catctagcca aaagaaagcg gcagtacaca aaataggggg
      961 acaagagcat ctccatttgt gggaagctga aaatccaact gaagaagtcc gacaattagc
     1021 tgttgaaatc agacgactag tcagtgaaga aaactaccgt tatcgtgata tccaagtgct
     1081 gacaaaaaat atgtctttat atggaaatat ccttcggccg atctttcaag aaatggcaat
     1141 tcctttttat atcgatgaag aacaaatgat ggaaaatcat ccgctgatcg aatggatcaa
     1201 cagtctgttc gctcttgacc gctattctta tcggctaagt gatattatgc gttttttcaa
     1261 aactgaatta tttctgtcga cggaagcaga aaccgaactt gatgaatgga aacaagagcg
     1321 agatgaattc aggaaaaaac tggacatcac tgaaaatgta gcacttgctt acaattatca
     1381 aggaacttat tggactcgtg aaaaagactg gcagtttgtg gcgtatgatt ttgaagcaga
     1441 acagattgaa gacgtacagt atttggaaga acagtcaaat acgattagac gattatttca
     1501 gaaagctgta ccagtatttt tgaaaaaaat aaagaaaagc aaaacaggac aagaggccgc
     1561 aaatgttttt tatcaatttt tgatccaaag tggcgtacag caacaattat tgttttggcg
     1621 taatcaggaa gtagacagag gaaacttaga agcggcaaga aaccatgagc agacatgggg
     1681 agcgctgatt gatctgttgg atgaatatgt agtaatttat ggtgaatcgg cttttgactg
     1741 gtcactgttc caagaaatca ttacaagtgg tctagaaaat ctaagttatg gtaaaatccc
     1801 taccgcaatc gaccaagtgc gaatcaatcg tttggaatta gttcgaccaa atcaagctgc
     1861 cgtcactttt gcattagggt tgaatgatca agtgtttcca gaccgaaaag agacaaaagg
     1921 attgttgtcc agtgaagaac gagaagtatt aaatcaggcc ttgccagatg gacagttttt
     1981 attcgaccca tcaaaggaaa gtatctcttt tgagcctttc caagcctatc ttgtttttct
     2041 ttccggaaca gatcgattat atcttagtta tgcacaaagc tatgacacag atggttcgct
     2101 gaagatgtct ccttatttgc gtagaatcac agaatattta tctgttcccg tcgagagaaa
     2161 agagcatttg acgatcgaaa gcgaccctaa ttgttatgta gggacttaca gaagcggaat
     2221 caatacagtc aataggattt atcgattagc tttagatgaa aaaaaacctg taccaaagca
     2281 ctggcaaaag ctcaaagaat tagtactgaa ttcgtcatgg cgaaattttg ctttacagat
     2341 ttttgaaagt caggaacatt taaatgttcc tgtttcgatg ccagaaaaaa tggcggaaga
     2401 gctttatgga aaagagattt atacttcaat ttcacgtatg gagaattttt ataattgtga
     2461 atacaaatac ttcatgcaat ttggtttgaa attgaaagaa cgaactgttt atggtctaac
     2521 tccagcagca actggtgatt tttatcatga atcattggat cgtttcttca agctactgtt
     2581 ttcaaaccaa ttatccttag tgaatatgtc cgatgcgcag cgtagagact ttactgaaaa
     2641 agtgctgcag gaagtattcg gagaacttcg gtttgagata ttggatagtt ctgcaagaat
     2701 gaactatatc cgctaccagt taggacagac gattcaaaaa gtgacatggg ctttgcaaaa
     2761 acagagcaaa aatacaggca tgcatcctct tcagacagag attctatttg gtcagattgc
     2821 tggagcaaaa ggtatcacag gtctggaatt accgttgagt aatggtggaa aactccatgt
     2881 tcgagggaaa atcgatcgaa tcgatgtagc ttctgaacaa gaagatactt ggctaagcgt
     2941 cgttgattac aaatctagcg gacgttcatt cgatgtcaca gaagcttatt atggcatggc
     3001 tatgcaatta ttgacgtatc tggacgtagc attgatggat gcggtacgtt tgactggaaa
     3061 ggcggctgtt aaaccagcag gatcatatta tttacatgtt cataatcctg tattgacgga
     3121 gacagatgat gtagagaaga atacattgaa aaaatttagt tatgatggcg tttttgtcga
     3181 tgatcccgct cttttagaag tactagatca ttcactggaa gcaaaagaac aatcactgat
     3241 ctatccagtg aaaaaaaatg cgaaagatga ataccaaaaa ggtagtttca gtaaagacaa
     3301 atttttcacg gaagaagaac tgcaactatt catggcgcat aatcgggaaa atatgcggtt
     3361 agctggagat gcgattactt ccggagaaat caaactaaat ccagcttata aagataaaca
     3421 gcggattgcc tgcgaatttt gtccattcag aagcatctgt acgtttgatg tgatgctgaa
     3481 agaaaataat taccaccgtc tggagaaaat agataaggaa gaagcactgc gtagaatatt
     3541 gaaaaggagt gaagaagagt gaatcaaatt cctttaaaac cgcaaaatga acgattcaca
     3601 gatgatcagt ggcaagcgat ttttgatcaa ggagataatc tattagtctc tgcgtctgcc
     3661 ggatcaggta aaactacagt gcttgttcga agagtgattg aaaaactaaa aatgggtttt
     3721 gatatcgatg aattattgat tgtgacattt actgaagctg ccgcaagaga aatgaaagaa
     3781 agaattcaag aggcattaca agaatccgtc aatagcgaaa gtgaccctgt acgtagacag
     3841 cattttacta agcagttagt tttattgccc acagcgaata tctcaacctt acatgctttt
     3901 tgcctgacag ttattcgacg ttattattac ttaattgata ttgatccagt ctttcgcatg
     3961 ttaacagatg aaacagaaac gatcttgatg aaggaggatg tgtgggacga gctgagagaa
     4021 gcactttatg cagaaaatga tgaacgattt ttccaattaa caatgaattt ttctaatgat
     4081 cgttcagatg acggcttgac gaatctggtg ttttctcttt atgagtttgc ccgtgccaac
     4141 cctgaccccc aaaaatggct agaacagtta agcgataatt atcgtttgcc tgaggggcta
     4201 gcgaaatctc gattatatca aaaacaaatc cgtcctttag ttttggcaga tatataccaa
     4261 tgtgtacagt tatatgaaca gatgactcag ttagcacaag gtgaaggatt agaaaaaatg
     4321 catgagcaag tagctggaga acaacagcag atcaagaata tctatgaagc gttctcgcaa
     4381 gatcggttag aagaagctta tgctggtttg gaacaactaa ctttttcaac ttttaagagc
     4441 agtcggaaag cagaattgaa agaaatttcc aatgaagtca agggcatgag ggataaagca
     4501 aaaaaactga ttcagcagat aagtaaaagt tattttcctg tttccccaag ccaaatggaa
     4561 gaattaacgg acaaagcact accgttagtc gaagaaatga caaaagtcac gcaaagcttc
     4621 atggacggtt tcagtatgcg taaacgggaa aaaggtgtgc tggatttcaa tgatctggaa
     4681 catttagcgt tgcagattct cacagaaaaa acgaaagatg tatggctgcc aagtgaagct
     4741 tcaaaacatt atcgaaaaaa attcaaagaa gtaatggtag atgaatatca agacgtgaac
     4801 caattacaag aagccatttt atactggcta agagaacccg atgatacaaa aggaaatatg
     4861 ttcatggttg gggatgtcaa acaatccatc tattctttcc gtttagcaga tcctagttta
     4921 tttatcggga aatacgagaa tttctcaaag aaagaaggcg gacgtcgaat cgttttggct
     4981 gagaacttcc gctctagaaa agaagtcctt tcttttacga atcttatttt tgaacaacta
     5041 atggatccag cagtgggtca aatcaattat gacgaagcag ccaaactgat ccaaggattt
     5101 tcagactttc cagagaatga acagtttgag ccggaaatca tgatctatga aaaagaacag
     5161 gaagaatcag agattgagat tccgacggat gatatactgg aagacaaaac agaaggcgaa
     5221 ctttttatga caggactaaa aattcgtcaa ttaattgatt cctcctttat gatttatgat
     5281 aaaaaatcaa aaaaaagtcg tccgatagag tacaaagaca ttgttctctt gacacctaca
     5341 aagaagaaca acttaacgat tttagagatt ttcaaaacat tggatattcc attagagatg
     5401 aatgatgctc aaaactattt tcaagcgaca gaaattcgca cgatgatctc gctgctacaa
     5461 ttaatcgata atccctatca agatattccg cttgcggctg tattgcgctc accgatcgtg
     5521 ggtctgattg aaccagaatt agccagcatt cgattagcta accgtgcaca tacttactat
     5581 gatgctgtgc ttgcttatca agcatcaaac gaagatgaat tagcagctaa gctggaacat
     5641 tttggcaagc aactagagca ttggcgagaa cttgctcgcc gttcttcgat cactgacttg
     5701 ttatgggata tctattatga gacgggatat ctagagtatg tggtcggtct gccagcaggg
     5761 gcacagagac aagccaatct ttatgcattg gttgatcgtg ccaaagctta cgagcaaagt
     5821 tctttcagag gattatacca gttcgtgcgt ttcattgaaa aaatgcaaga aaaagataaa
     5881 gacttagcag aaccagtcat ttcaatagaa gataatgctg ttcgcgttat gacgatccat
     5941 gcaagtaaag gcttggaatt cccagttgta tttttgttag atatgacaaa ggagttcaac
     6001 ttacaagact tgcgcaatcg gtatgctttt gaagaaaaac ttggagcagg aattcgctat
     6061 atggatcctg aaacacgagt gttatatgat acgttaccgt ttcaagccat caaattggca
     6121 aagcaaaaca aattgttgtc ggaagaaatg cgtaaattgt atgtaggatt gactcgggca
     6181 gaacaaaagc ttttcattgt cggttcctat aaaaataaag agcaaatgat ccaaacgtgg
     6241 tctgaagcag ctgatcatga ggagttagtt tttgatcctg cgctacgttt gaagggcaga
     6301 agcagcttga tgaattggat tggttacgga ttgatccgtc atcctgaaat gcagaaatac
     6361 ttagaggaag aaatttcgac ttctcttctt caacattcaa atgcacagtt ttctatttca
     6421 tggatgaatc agcaatcgat cattgaacaa agacagttgc ttgctgaaaa agaactggtt
     6481 aacttagatc agcagatgaa agaagatgaa actttattag cagattcgtt acaaaaacgt
     6541 ctggcatatg aatatcctta tcaagcttct tcacaaacca caagttatca atctgtatct
     6601 gaaatcaaac gattattcga agatccagat gatacacaag aatcacgttt gactctggaa
     6661 agcagtcaga acaaagcagc atcccgtcaa tttcgttata cgcaagaaca actagcagaa
     6721 ccaaaatttt tgcaaaaaga tcggcaagtg tcagcagctg ctgttggaac tgctacgcat
     6781 gctttgttac aattactgcc attagagatg ccaactacag agagtatcca tcaaaaatta
     6841 caggaattgg taaggaaacg gttagtagac gaaaaagtcg cgaaaaaagt agatgtttct
     6901 tctattatat ggttctttca aacagaactt ggtcaacagc tgattgccaa caaagaaaac
     6961 gtaaaacgag aacaaccttt ttccatgctg cttcctgctg acgaagtatt tcaagactat
     7021 ccaaatcaag aagatgaact attaatccac ggaatcgtcg atggatacct agaagaaaaa
     7081 gatcatctca atatctatga ttttaaaaca gattttattt tacatccgga tgatccagca
     7141 gaaatagatg caatcgtcca aaaatatcaa ggacagctaa gactttacca acaagcaatg
     7201 tcagaagctt tgaataagcc agtagaaaat gttttcctaa ttttgcttcg agtgaaacaa
     7261 attataaaca ttaacaaata attataattg agcaaacttg acttatgttt tgtaaggatt
     7321 atgctatact ctgggaagag gtgagtatga tggagctaac acagacagaa ttcgttttat
     7381 gccctaaatt tgaaaaaaca ttctctattt taggaaaaaa atggaacgga ttgattatcg
     7441 atgtactact cgaaaacggt ccgcaacgct ttgttgagct tgcggcaaaa atcccgatgg
     7501 tcagtgatcg agtattagtc gaacgcttga aggaattaga aaaagaagga atagtcactc
     7561 gaaactttgc ctgtagcgaa aagagccgtt cagaatatct cttgactgaa aaaggagcag
     7621 acttgcaaaa agcgatgcgt caaatacaag tttggagcga aaaatggatc acagaggaag
     7681 aatgcggttg attttgtaaa aaaaatcttg taaactatag tccatacgaa taagtaaaga
     7741 acgatgacga aaaagagtag tttggctaaa ccagttcagg gagatctgtc gcagactgaa
     7801 agcagatcct gtgtttgaaa aatgaagttc actttccgag ttcgtctgat aaaatcagac
     7861 cggcaattgt cgttaacgaa gtaagagtgc ggtgtaagtc atttatccgc aaattaggat
     7921 ggtaacgcga agacctcgtt ccttaacagg aatgaggtct ttttgttttg tatttggaat
     7981 ttaaaggagg aacaagcaca tgtctctaca agcacaatta gagagcttga aagaagcaac
     8041 gctcgtcaaa atcacagcgg cacaagattt aaatgaatta aatcaaatac gtgttgaaac
     8101 gttaggaaaa aaaggaccga tcacagaagt attacgtggg atgaaagatc taagtccaga
     8161 agagcgacca aaagtcggaa gtttcgcaaa tgagatccga gatcttttga cagaagagat
     8221 cgagcgaaaa aaacaagtat tagaagaaga agcgttaaat gcagctttgg caaaagaaac
     8281 aatagacgtg acactacctg gtagaaaagt aaaacatggt acacgtcata tcgtgacaca
     8341 aatcatggaa gaaattgaag atatttttgt gggtatgggc tatcagatcg tagaaggata
     8401 tgaagtagaa tctgatcatt acaatttcga acggatgaat ctgccaaaag atcatccagc
     8461 aagggacatg caagatactt tctatatttc agacgagata ttgattcgga cacatacttc
     8521 accagtccaa gcacggacaa tggaaaaaca tgatttctct aagggtgctc tacgaatgat
     8581 ctcgccaggg aaagtcttcc gcagagatac agatgatgcg acccacagtc atcagttcca
     8641 tcaaattgaa gggctagttg ttgataaaaa catcacaatg ggcgacctta aaggaacatt
     8701 agaagtcgta atgaaaaaaa tgtttgggga agatcgtgaa attcgtttgc gtccaagtta
     8761 tttcccattt acggaaccat cagtagaagt agatgtcagc tgtttcaaat gtggtggtgc
     8821 cggttgtaac gtatgtaaat acactggatg gatcgaaatt ttaggagctg gcatggtaca
     8881 cccgaatgtg ttgaagatgt caggaatcga tccagaagaa tattcaggct ttgctttcgg
     8941 ccttggacca gaccgcgtag ctatgttgcg gtatggagtg aatgatatcc gtaatttcta
     9001 tcaaaatgac ttacgtttct taagtcagtt caagggggag taagaaaatg ttagtttcat
     9061 ataaatggct caatcgttat gtagatttat cgaatgtaac accaaaagaa ttagcggata
     9121 agatgtctgt gacaggaatc gaagtagagg gagtaacggt tccagaagaa ggcttgaaaa
     9181 aaatcgttgt cggtgaagtc aaggaatgcg taccacatcc agattctgat cacctttcta
     9241 tttgtcaagt cgatattggc gaagacgaac tgtctcaaat cgtttgtgga gcgcctaatg
     9301 tgaaggttgg aatcaaagtg attgttgcac ttcctaactc acgtatcgct ggtaatgtaa
     9361 aaatcaaaaa aggaaaaatg cgtggccaag tatcaaatgg catgatttgc tcattacaag
     9421 aaattggcta ttcagatagt gttataccga aagaatacgc tgaaggtatc tattatatgc
     9481 cacaagaagc tgtaaatggc gatccagttt ttccttattt ggatatggat gattcgatta
     9541 ttgaattatc tatcacaccg aatcgtgcgg atgcattgtc aatgcgtggc gtagcttatg
     9601 aagttggggc aatttatcgt cagactccta cattcgatga cgaaaagctg gtagaatcct
     9661 cacgtcctgc tagcgataaa attcaagtgg aagttgaaga taaagaagca gttcctgctt
     9721 atcaaatcag aatcattgaa ggagtaagaa tccagccaag tcctcaatgg ctgcaaaatc
     9781 ttttgatgaa cgaaggcatt cgtccaatca acaatgtagt agatgtgacg aactatattt
     9841 tgcttttatt cggacaacca ctacatgcat ttgactacga caaattgaaa tcaaaagaga
     9901 tctttgtccg ccgtgcaaaa gaaaatgaaa cattagtaac actagatgga gaatctcggg
     9961 aattgacaac tgacaatctc gtcattacta acggcaaaga accagttgct ttagctggtg
    10021 tcatgggagg gttagactca gagatcacga atgaaacaac aacagttgca ctagaatctg
    10081 ctttatttga tccaatttcg attcgtcgta cagcaaaaca atttaatttg cgtagtgaat
    10141 cctctgcacg ttttgaaaaa ggaatcaacc atgcgactgt tggagaagcc agtgaggttg
    10201 ctgcagcaat gatcgtttct cttgcgggtg gggaagttct acaaggcgcg gtcaaaggct
    10261 cagagatcaa agcagaagac gtatcagtga agatttcact tgaacgcatc aatcaatatt
    10321 taggaacatc tcttgtcgtt aaagaagtaa atgagatttt tgaagcactt ggatttagct
    10381 atgaagaaaa ggagtatgtt tattccgtag tagtaccacc aagacgctgg gatatccaaa
    10441 tcgaagcaga tattatcgaa gaagttgctc gtatttatgg atacgaccag ttgccatcta
    10501 cacttccaag tggagaaacg gttgctggaa gcttgtcaaa agaacaagaa actactcgta
    10561 aaattcgtac aatctttgaa ggaagcggat tgagcgaagc aatcagttat gcattgacaa
    10621 cagaagaaaa atcaagacag tttgttataa atgaaagtaa tattacacgt ttggactggc
    10681 caatgagcga agaacgttct gtattacgaa tgaatttgat cagcggacta ttagatgata
    10741 tcagttataa cactgcccga aaaaatacag agattgcttt atatgaaatt ggtcgggtgt
    10801 tcttccaaga aaatgatcca cgtacacatc tgccgcaaga agtaaaacat gcagcaatcg
    10861 ctatttctgg tatttgggaa gaaaaagatt ggcagacgaa agctcaaaat gtagactttt
    10921 tcaccatcaa gggtctttta gaaaacttat ttgaacaact aggcatcact gaagatgtcc
    10981 attatcaaac agtgacagaa atgaaggaaa tgcatcctgg aagaactgct gcgatttacc
    11041 ttggcgaaaa atttattggt tttgtcggtc aagtgcatcc aacgattaca aaaagttatg
    11101 atattccaga aacatatgtt gctgagtttg atttatctgc tatagtggaa gctgctcaaa
    11161 aagggatcgt tttcgaagca gtgacaaaat tcccagcagt cagccgggac atcgctttgc
    11221 ttgtaaaaga aactgtgaca aatcaggaat taacagaagt tatccgttct gctgccggaa
    11281 gatttttaac agatatccaa ttatttgatg tctatcaagg agaaaacatt gaaaaaggac
    11341 ataaatccat ggcgtatagc ctgacattcg tcaacccaga agcaacattg acagatgaag
    11401 aaatcaataa agcaatggaa aaagtcgaaa aagcgttggt agaaaaccta gaggcaagta
    11461 ttcgctaaga aggaaaatat agtaagtaac gatcaagtag cttttgaaga ccatacattt
    11521 gaaaaaaaga tgtgacacaa cttcgtcaca tctctttttt tgattttaaa aaaggaatag
    11581 cacagagacg agataaaaaa acaggccttt acagcctgct tttttagagt aaatgaatta
    11641 atggaagtaa ttgattccca ttgctgattt tacttcagaa agtgtttgtg cagctacttt
    11701 agcagcttct tcactacctt tacgtaagat ttccataaca gctgaaggat ctttttccaa
    11761 ttcttctcgg cgcgcacgga tcggtgcaaa ttcagcttcc aatacatcaa tgaggtatcg
    11821 tttgatcttt acatcaccaa gtccgccgcg gcggtaatga gctttcattt cttcaattgc
    11881 ttctttatct gttccgaata catccaagta ggtgaagacc atattccctt ctacttgtcc
    11941 aggatcttgt acatggatgt ggtttggatc agtatacata ctcatgactt ttttctgtaa
    12001 gacatcggca ggatcggaga tataaatacc gtttcctaac gatttactca ttttgccatt
    12061 tccatcgatt ccaggtaatc tacccattcc ttttggtggg aatacgcctt ttggttccac
    12121 caagacttca ccatatgtat gattgaagct ttgtacgatt tcctgtgtct gctctagcat
    12181 tggtttttga tcctcaccaa caggtacaag attcgctttg aatgcagtga tatctgctgc
    12241 ttgagaaaca ggatagataa agaatcctgt aggcacgctt tcaccgaatt ttttttgttc
    12301 gatttctgtt ttgacagtcg gattgcgccg aacgcgtcct acgctgacta agttcaaata
    12361 atacatggtt aattcagcta attccggaat ttgtgattgg ataaataaag tagattttgc
    12421 aggatctaat cccacggcta aatagtctaa agcaacttgc aagacatttg atgaaacctt
    12481 ctcaggattt ttagcattat ctgtcagtgc ttgcatgtca gcaatcatga caaacagttg
    12541 gttgtcttca tctgcctgca tagccactcg ggtttttaga gaaccgacgt aatgccctaa
    12601 atgcagcttc ccagttggtc gatctcccgt taaaataata tttttcaaaa aagacacctc
    12661 tttacgattt gtaagtctga gcacttaata tcttaccgtt tttcctgaaa agaaacaaca
    12721 gttcctaagg aaaaaacaag gaaagaggat aaaataacta atagattaga aatggaagaa
    12781 atatttatgg gagaataaac gttctaagaa actttacaaa ttactaaact atcagatata
    12841 atgtatttta ttatacatct ttaaaagaaa tcttactaaa taataatggg agtgtaattc
    12901 tatggcaatg attaaatttg atcacgtgga taaatattat ggcaagttcc acgcattaaa
    12961 aaatatcaat ctcgaattcg agaaaggaga agttgtcgtt gtcattggtc cttctggttc
    13021 aggaaagagc acgatgcttc gctgtatcaa tggattagag acgatctctt caggtaagct
    13081 gctgattaat gatactgact tgcatgataa aaagacaaaa ctgacagaag tccgaaaaaa
    13141 catcggcatg gttttccaac atttcaattt gtacccaaat aaaactgttc tggaaaacat
    13201 caaattagca ccaatcaaag tgttgaagca agaccaggca actgcagtca aaaacgcgga
    13261 gaaatttttg aaaactgtca atatgttaga taagaaagat tcttatcctt ccatgttatc
    13321 cgggggacaa cagcaacggg tggcaatcgc cagaggttta gcgatgaatc ctgagatgct
    13381 gctgtttgat gaacctactt ctgctttgga tccggaaatg attggtgatg tattagacgt
    13441 tatgaaaaaa ttggctcatg acggtatgtc aatgattgtc gtgacacatg aaatgggctt
    13501 tgccaaagaa gtggcggatc gtgttatctt catggctgat ggagaagtat tggaagacag
    13561 cagaaatgtg agagactttt tcgaaaatcc aaatgaagtc cgagcacagc aatttatcag
    13621 taaagttatc aatcattgat aggaggaaaa agatgcgcag gttaaaaaaa atacgcctga
    13681 ttttcctttt aggcattgct tttcttgcac tagccggatg taaaggaaat gggttggcaa
    13741 atgaagatat tttagagcga agcaaatcta ccaatgaaat catctggggt gtcaagtatg
    13801 atactcgatt atttggaatg atggatattg agagcagaac tgtacaagga tttgatgtag
    13861 atattgcaaa agcaatcact aaaaagatcc ttggtgacaa tggaaaaaca gaatttgttg
    13921 aagtcacttc taaaactcgg attccattat tgaaaaacgg aaatatcgat gcaattattg
    13981 caacgatgac gatcacagat gaacgaaaaa aacaggttga cttttcagat gtctatttcg
    14041 atgctggcca agctttgttg gtaaagaaag gaagccaaat caaaagtgta gatgatttga
    14101 atgcttctac aacggttcta gcagtcaaag gttctacatc ggctgcgaat atccgacagc
    14161 atgcaccaga tgcaaaaatt ttagaattag aaaactatgc agaagcattt actgctttac
    14221 aatctggtca gggagacgcg atgactacgg ataatgcgat tttgttaggg attgcagatg
    14281 aaaatccaga gtatgagtta gttggtggaa cgtttactaa tgagccttac gggattgcga
    14341 tcaacaaagg gcaagaaaac ttcttgaaag ctgttaacca agcgctagag gaaatgcatg
    14401 cagatggcac atacgataaa atctatcaaa aatggtttcc aaatgaaact gaaggaaaag
    14461 tagagtaagg gggagaggca atgagcgaat tgatacaaac ttatggacca atctttgtcg
    14521 acgggttcaa gatcacattg ttttcgagtg tcttagcact tgtgtttagt ttgattatcg
    14581 gaacgttaat ggcaatcttc caattatcaa agaataaagt agtgagcgcc ttagcaaagg
    14641 catatgtaga attttttaga aacattccat tattaatcat cgtgatgttt ttttatgttg
    14701 tcgtaccgat ttattggttt aatctagatg gattccaagc aggtacgatt ggattaacga
    14761 tctatacatc tgcttttatt gctgaaacag tacgttcagg gattcaaacc gtaccaaaag
    14821 gtcaaacaga agcaggacta tcttctggtt ttacctattc agaaatcatg cgttatattg
    14881 ttttaccgca agcattcaaa atcgttatcc cgccattagg caaccaattt atcaacctcg
    14941 taaaaaactc atctatttta gcaatcgtag ctggtttaga tttgatgtat caaggagact
    15001 tgatcgctag tgaaacattc aataccttcg atacgtatat catcgttggg ttattttact
    15061 tagttatcac gctaccgtta tcttacttaa tggtttatct tgagaaaaaa tgggcggttc
    15121 gttcatagaa ggaggggaaa tacatggatt ttagcggcgc tttttcatgg atgaatatcc
    15181 gttttttatt ggaaggatta aaggttactg ttgaagtttc attgttttcg atcattttta
    15241 gtttcttgat cggtggtctg acaggtgtga ttcgttttgc gaatatccct ttcttttcca
    15301 aaatccttgg tgtagtgatt gatattatac ggaatcttcc attattattg atcattttct
    15361 tcacttactt tgccttacct caaatcggca ttcagatgaa catcttctgg tccgctgtcg
    15421 cagcattaac gatttttgag tcagctatgt tgtcggaaat tttccgagct ggactcaatg
    15481 ctgttccaaa aggacagatg gaagcaggat tatcaacggg actgacttat gtagaatcga
    15541 tgcgaacgat cgtgttaccg caagcattta aatcaatgat cccggcaatt gtgagccaat
    15601 tgatttcact gatcaaagat acttcattag cagtaatcat ttctttacct gaactttctc
    15661 atcaggcacg tattatttat ggacagaaca ccaattatgt gcttccgatg ttcatcatca
    15721 tgacacttat gtacttcgtg gtatgttacg cgctttctct gctttccggt tacttggaaa
    15781 agagacggta cagttattaa tagatgattc ttttagcggt tgtggcaaac taaagtcaca
    15841 accgcttttc ctctttccat tcgaaaaaaa gagatttcga ttgttttaag caagtaagag
    15901 cacttgaaaa ttcacgctaa aaagtacaca atagggagga tagaatttat tgaaaaaaag
    15961 gatgatacga ttgacagata atcgccctat cggatttatt gattcaggtg tcggcggctt
    16021 gactgtagta aaagaagccc tgaaacaatt accgaatgaa aatattttat ttgtaggaga
    16081 cacagcacgc tgcccatatg gccctagacc cgcggaacag gtaatccagt atacttggga
    16141 aatgacggat tatctggtgg agcaaggaat caagatgctg gtgatcgcct gcaataccgc
    16201 aactgcggtg gctttagaag aaatcaaagc tgctctttct attccagtca tcggtgtgat
    16261 ccttcccggt actagagcgg cagtaaaaaa aacacaaaat aaacaagttg gcattatcgg
    16321 tacgattggt acggtaaaaa gtcaagctta tgaaaaagca ctgaaagaga aagtaccaga
    16381 attgactgtg acaagtcttg cttgtcccaa atttgtttca gttgtcgaaa gtaatgaata
    16441 ccattcatcg gtggcgaaaa aaattgtggc agaaacatta gctcctttaa ccactaaaaa
    16501 aatcgataca ttgattttgg gatgcaccca ttatccatta ttacgcccca tcattcaaaa
    16561 tgtaatggga gaaaatgttc aactgatcga ttctggagca gaaacagtag gtgaagtatc
    16621 tatgctgtta gattatttca atttgagcaa ttcaccgcaa aatggtcgga cattatgcca
    16681 gttttataca actggctctg ccaaactttt cgaggaaata gctgaagact ggcttggaat
    16741 cggacactta aatgtagaac atatcgaatt gggaggaaaa taaggatgcg tcatgacggg
    16801 agaaaagccc aagagcttcg caaactggag cttcaaacaa atgttttcaa atatccagaa
    16861 ggatcggtag tgatccgttt tggtgataca acagttattt gttctgcaac aatagaaaat
    16921 tctgttcccc cttttttgag agaaacagga acaggatggg tgacagcaga atatagtatg
    16981 ctgcctcgag caacgagtac gagaaatcgt agagaaagca gcaaagggaa actttctggt
    17041 agaacaatgg aaatccagcg tcttattggt cgttccttgc gtgcagtagt agacttggaa
    17101 aaattaggag aacgtagtat catcgtagac tgcgatgtga tccaagcgga tggcggtact
    17161 agaacagcaa gtatcactgg ggcatttgtg gcattgaaat tagccattga aaaactttta
    17221 agagaaaaag aattgtcaga agatccaata aaagaacatc ttgctgcagt tagcgtagga
    17281 atcttaccag atgggacttg cgtcacagat ttggattacc aagaagattc cgcagcactt
    17341 gtagacatga atcttgtaat gacagaatct ggaaaattcg tggagattca aggaactgga
    17401 gaagaagcaa cgtttgatgg tgaacagttg aatgaaatgc tcttttttgg aaaaaacgcg
    17461 attgaagatt tgatcaaaga gcaaaaacat gctttgctaa cagagtttgc tcaaaatgat
    17521 gagcggatag aagaaacaaa aacaatcatc attgcgacaa gaaatccagg aaaagcagaa
    17581 gagtttcgaa atatgttcaa agaagcggga tatcatgtaa aaacattact tgactatcct
    17641 gaattgccag atgtggaaga aactggcagc acatttgaag aaaatgcccg attaaaagca
    17701 gaaacgattg cacaattgct tgatcagcca gtcttagcag atgattcagg tttgaaagtg
    17761 gatgctttag gcggtatgcc aggaatctat tctgcacgtt ttgcagggga acaaaaaagt
    17821 gatgcgggga ataacgccaa attattatat gaattgacag atgtgccgga tgaaaaaaga
    17881 acagcacaat tccattgtac gttagtattc gcggcaccca aaaaagacag ccttgtagta
    17941 gaagcggaat ggcccggtcg agtagcccgt ataccatctg gcgaaaatgg ctttgggtat
    18001 gatcctttat ttatcccaga aggtaagaaa caaacagcag cagaattatc gagcgaagaa
    18061 aaaaacaaga tcagtcatcg ggctcaagca atgaaaaaat tgagtgccga gtggaaacaa
    18121 tggctggaag gagagagata gatgcggtat ttagtagtaa gtgataatca tggagacaga
    18181 gatatcctcg ttgacttagt agatcgttat cgttctgaag tggatatctt tttccattgt
    18241 ggtgattcgg aattagaagc ttctgaccca ttatgggata cattccaagt ggttcaaggt
    18301 aattgtgatt atggaccagg attcgaacag aaaaaagtga tccaaactgg tcaggataca
    18361 gtattcatga ctcacggtca tttatcgaat gtccgatttg gtttgacaca gttagcaatc
    18421 gaagctcgag ctgcaactgc cgatatggtg ttttttggtc atacccatca aataggctgt
    18481 gagatggatc agaaaatcct gtttttgaat cctggtagca tttctcagcc tagaggcccg
    18541 atccaaatcc catcatatgc tcttatcgaa agtttagatg accaaatttc tgttcaatac
    18601 tataaccgtg cccatcaggc agttgaagag ttggcctttt ctttttcgag agagcgctga
    18661 ataaaaaaac tctaattttt aataaaaata agctttattt tatgaatttt gcatccggtt
    18721 tcggtaaaat agtagtgttg aatgaaatag aaatggaggc catcagatga ttggacctat
    18781 agtaagagaa atgctgctgg aaaatcagga aacgtttttg attcctgcag aaaatgtggc
    18841 aaatgttatg tatcatcatc cattgtccca tggtttactt gtgttatcga aggtcggtta
    18901 ttccaaaatt cctgtattag gaaaagatga tcactttgta ggactagtaa gtttatcaga
    18961 tgtggtcaat aaaatgattg acttgcaaac aatcagcatg gacccgttag aaggactaac
    19021 ggttgctgat gtgacagaaa cagatgttcc gacaattgat gaaaattggg aactagaaga
    19081 agtactccat ttgttagtcg attcgccttt tttaccggtt gtcacagaag atcggatttt
    19141 caaaggaatc attacaagaa aagagctgct taaagccgta aacttcatgg tgcatgaatt
    19201 agaacgtcgt aatatattgt ctccgaaaat cgaagaatta aaagaacaaa tcgatatcgt
    19261 gagctaaaga acgagaaact aaaagataat caaaaaataa gtagaaaaaa cagcgtacca
    19321 gttttgactt tatcttgaaa aagaggctgg gacaaaagcg tttaactcca agaaataaga
    19381 aggaattcac gaaaattgct tttcaaattt
//
LOCUS       DAZUMT010000055        18564 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000055 DAZUMT010000000
VERSION     DAZUMT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.055"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8FM_001711"
     CDS             6..305
                     /locus_tag="ACG8FM_001711"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5094374.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8FM_001712"
     CDS             378..1739
                     /locus_tag="ACG8FM_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5094375.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8FM_001713"
     CDS             1761..2021
                     /locus_tag="ACG8FM_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094376.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8FM_001714"
     CDS             2035..2787
                     /locus_tag="ACG8FM_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS5094377.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8FM_001715"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8FM_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5094378.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8FM_001716"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8FM_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS5094379.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8FM_001717"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8FM_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5094380.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8FM_001718"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8FM_001718"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5094381.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8FM_001719"
     CDS             7161..8558
                     /locus_tag="ACG8FM_001719"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5094382.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8FM_001720"
     CDS             8639..9079
                     /locus_tag="ACG8FM_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5094383.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8FM_001721"
     CDS             9227..9553
                     /locus_tag="ACG8FM_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5094384.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8FM_001722"
     CDS             9554..10303
                     /locus_tag="ACG8FM_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS5094385.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8FM_001723"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8FM_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5094386.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8FM_001724"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8FM_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094387.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8FM_001725"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8FM_001725"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS5094388.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8FM_001726"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8FM_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5094389.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG8FM_001727"
     CDS             14770..17436
                     /locus_tag="ACG8FM_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5094390.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG8FM_001728"
     CDS             17800..18552
                     /locus_tag="ACG8FM_001728"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5094391.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT         5964 a         3212 c         3832 g         5556 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct tttt
//
LOCUS       DAZUMT010000056        18506 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000056 DAZUMT010000000
VERSION     DAZUMT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.056"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..1881)
                     /locus_tag="ACG8FM_001729"
     CDS             complement(115..1881)
                     /locus_tag="ACG8FM_001729"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094392.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1841..3595)
                     /locus_tag="ACG8FM_001730"
     CDS             complement(1841..3595)
                     /locus_tag="ACG8FM_001730"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094393.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3705..4274)
                     /locus_tag="ACG8FM_001731"
     CDS             complement(3705..4274)
                     /locus_tag="ACG8FM_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS5094394.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4292..5707)
                     /locus_tag="ACG8FM_001732"
     CDS             complement(4292..5707)
                     /locus_tag="ACG8FM_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094395.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5718..6386)
                     /locus_tag="ACG8FM_001733"
     CDS             complement(5718..6386)
                     /locus_tag="ACG8FM_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFS5094396.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6517..7101)
                     /locus_tag="ACG8FM_001734"
     CDS             complement(6517..7101)
                     /locus_tag="ACG8FM_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5094397.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7436..7666)
                     /locus_tag="ACG8FM_001735"
     CDS             complement(7436..7666)
                     /locus_tag="ACG8FM_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5094398.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(7681..8631)
                     /locus_tag="ACG8FM_001736"
     CDS             complement(7681..8631)
                     /locus_tag="ACG8FM_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS5094399.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8628..9470)
                     /locus_tag="ACG8FM_001737"
     CDS             complement(8628..9470)
                     /locus_tag="ACG8FM_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS5094400.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(9603..10328)
                     /locus_tag="ACG8FM_001738"
     CDS             complement(9603..10328)
                     /locus_tag="ACG8FM_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5094401.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(10789..11103)
                     /locus_tag="ACG8FM_001739"
     CDS             complement(10789..11103)
                     /locus_tag="ACG8FM_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5094402.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(11119..12714)
                     /locus_tag="ACG8FM_001740"
     CDS             complement(11119..12714)
                     /locus_tag="ACG8FM_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFS5094403.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(12737..13627)
                     /gene="iolE"
                     /locus_tag="ACG8FM_001741"
     CDS             complement(12737..13627)
                     /gene="iolE"
                     /locus_tag="ACG8FM_001741"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFS5094404.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(13644..14669)
                     /gene="iolG"
                     /locus_tag="ACG8FM_001742"
     CDS             complement(13644..14669)
                     /gene="iolG"
                     /locus_tag="ACG8FM_001742"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFS5094405.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(14693..15700)
                     /locus_tag="ACG8FM_001743"
     CDS             complement(14693..15700)
                     /locus_tag="ACG8FM_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5094406.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(15813..16973)
                     /locus_tag="ACG8FM_001744"
     CDS             complement(15813..16973)
                     /locus_tag="ACG8FM_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFS5094407.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(17049..18503)
                     /locus_tag="ACG8FM_001745"
     CDS             complement(17049..18503)
                     /locus_tag="ACG8FM_001745"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS5094408.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         5708 a         3852 c         2881 g         6065 t
ORIGIN      
        1 catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta ttctttttga
       61 aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat ttcatcatcg
      121 tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct ttatgagatt
      181 tttgtatatt tctgaccttc gatacaactc actatgagta ccttgatcaa ctatttttcc
      241 ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat gcgatataat
      301 tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt tttcattttc
      361 cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt tcaaaaaagc
      421 ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt tttctccgac
      481 caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag ctaaacgagc
      541 tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat tttccaaaat
      601 acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa acaaagaagc
      661 tggagataca cgtttaatat catacccatc aattaaaata cagccttcat cataatcata
      721 cagtctagac actaatttca agatactagt tttcccggaa ccactagcac ctactaacgc
      781 agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg tattgttatc
      841 atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac tatctgatcc
      901 ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag ctaaataaaa
      961 tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta ttgcggctag
     1021 taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt gaacacctac
     1081 tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca tgactgaaaa
     1141 agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt tgaaaagtct
     1201 gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt ctatagtttc
     1261 ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg ctttcgtttg
     1321 ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa taacagctaa
     1381 tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa acagagccat
     1441 accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa cgtcagacat
     1501 gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg gtaaatttga
     1561 caaatcgtct gcaatttgca ttcttaaatg cgcactttct ttataagtac tattatataa
     1621 gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacga acgatactgt
     1681 aaaataaagc caaggcggct ttacattttc taaaactaat tcgtctatca gcatcattat
     1741 aatgaatgct ggtaccatat taatgcagta tacaaaaaag ctagaaatag atgctttaac
     1801 caaatcttct gcgccctgat tagttaaagc gaatttgttt ttataaaaac ttttcattgt
     1861 caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac acttgatgtt
     1921 tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac aagacaagaa
     1981 tttcatctac agatttaatg ctggataatc gatgagctat cataataact gttttttctt
     2041 tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca atagatgcac
     2101 tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca atagctatac
     2161 gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta tgctctcttt
     2221 cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca aatactttac
     2281 ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga ttgaatagtt
     2341 tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa tagtgattta
     2401 aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct gaaattagtt
     2461 tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt cttttttgtt
     2521 ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag ctgacatttt
     2581 caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt tggtcttgct
     2641 tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca aacaagtgca
     2701 tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa aaaaacaaca
     2761 tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa aaggttatag
     2821 gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca aaaggtgttt
     2881 tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct ttattcaagg
     2941 ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattcct cggatatatt
     3001 ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata aaatttgtat
     3061 tctctgtcat tttctttaac aagaatacac caattactaa catggcgatt agaaccaatc
     3121 caattttaaa attaatgaaa aagcctaagg caactgataa aacaggtgtt actatggctt
     3181 gagcactatc tggaatcaag tgagcaattg ccatatgtgt tttagccgcg ttgtcatcaa
     3241 ttgtctttcg aatatatccc gaagaattta aatcaaaaaa acgaaaactt gcttctgtta
     3301 aaccgctaat tccctttttt ctcaaattag tctctaagcg aaacccaagg atatgagaaa
     3361 ataagccaga aaaaatataa aataaagctc ctaaagttaa ccaagcaaca gtttgaaccg
     3421 cataactctt agctaaatat tcatctccta caataattag agacttaaga aatttgtaaa
     3481 tactgtaata tccatagact gttaatacga cagaaaaatc agaaaataaa gtggctaacc
     3541 atccgtagta tttcatttta gggacataac taaataattt tttgtatact ttcatttttt
     3601 cctcccccgc attcaatttc ttctcttatt cgtttacaac tgttaatgct ttccgtaata
     3661 gcgtaaatta gagatgtaca aacagataga aggtaaacta cgttctattt tttgtatttt
     3721 tttaagactt ttcttccaat caatacacca atcatagata aacttgctac aactaacact
     3781 gaaatgattc cccatatacc taccgcatag tggatcattg catccattgt tgcagcagat
     3841 tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa agaaccatat aggaatccac
     3901 tgtccggcag cactgcctaa ttgcattaga gcccaagacg caacttgagg aattgctcga
     3961 tcatatccaa atacagatag aataatatca gctaagatcg ccatactaat cgaactgata
     4021 aaccaaggta agtacgcccc gcccattaac gtaaaaaata acaataaaac aattgtgtaa
     4081 atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat gattccagat
     4141 aataatccaa aaatccctgg ggatatgctg tgaccaaacg ccccaccaat actactaacc
     4201 attattgaaa aaaagtaaat tactagggaa ataactgtag ctaaaccaat aaaaatataa
     4261 tcttttgttt tcatatatat tctcctcaat tctatttcgt tatgtcatta aaaatctgtt
     4321 ctatcaaagt tgaatcttct actctaagtt ttatatcttt ttttattcca ccatttttta
     4381 aataaataat tctattgcat gttttataaa gaaattccgg atcatgagtg gctataacta
     4441 ctgcaccctt ttcttttaag tctttaataa ggtcagcaat cctttccata ttaatataat
     4501 ccaacccact tgtaggttca tcaaatagaa ataaattagt ttttgacaat gaagctatag
     4561 aaaccaataa tctttgtttt tctccaccgg ataaatcaaa cggtaattga tctttcattt
     4621 tataaagttc catttttttt aaaatttttt cttgctgatc tttactaact atttcattcc
     4681 ccagggctaa ctcgctcctc actgattctg tgaaaaattg gtaatccatt tcttgcatta
     4741 ctaaaaacgg cgatgacaaa ttttttttat tttcgattat ctttcctttg gtagggcgaa
     4801 taatcttcat gagtattctt agtaaggttg ttttccccac accgtttttc cctaccaaac
     4861 caataacatc tccaactttg acatcaaaat ttatgtcttt tagaatattt tcataagtaa
     4921 tttcttcaac ttttaataca tctgcactta ccttttcgat atagggaact tcaatcgaac
     4981 tattaaaaat gtcaaatttc cttaatgcgt tattgttttc agtttttaac aactgaatag
     5041 ttgtagtttt tacagttcta tttttaacaa atagaacttg atcaattaat tctttcaaat
     5101 agtaaaaacg atgttcagct ataacaatag taaatccttt attttttaaa tcacaaagta
     5161 agtcagcaag tttcttagtc gctttgaagt ccaaatttga agacggttca tccaatataa
     5221 ttaatttaga gtttagcgat aaactacatc caatagaaat tttctgccgt tcaccacttg
     5281 atagcgaaaa aatgttttta tctaatatat ttgaaagatc taataattca gctaattcat
     5341 tgattctttt atccatatgc tcttttgtaa atccaaagtt ttccatcgaa aatactaact
     5401 cactcgttgt atttgttgta aagaattgtc ctctaggatc ttgacttacg tagccaatat
     5461 tttttgatat actttcttgt gttaaggatt gtagcgagtt attttcaaat aaaatttccc
     5521 caaccaattt tccgtcataa agtcttggaa taagtccatt cattaacttt aatagtgtgg
     5581 atttcccact cccagaagaa ccagtaatca atgtaagact accttttttt attgaaaaat
     5641 taaggtcaac aagcgcttgt tccttaacat ccttatatga aaaagaaaca ttgttaaatt
     5701 caaacaattc tttcacctca aatcaaaata cttaagaaaa aaatagtaac ggaaaacagg
     5761 atgatatagt ctaataagct catgtttgtt ttatagtaag aacttctttt cacaggagat
     5821 tctatcccct ttgttattgc attagcggtc atttcttcag caatcatagt tgcacgtata
     5881 agaacgggaa ctaacagaaa ttccaaatat tgcagaggct gaaaaaaaga caagcgaatg
     5941 tttctcattt ttaaagattc tttaatcatt cttatttcag aacgataagt cggaaagaat
     6001 ctgatcacta ctgtaatact taacattatt tttttcggta aatgtatttt ttctaaagag
     6061 gataataatt cattgctagt atagacacta accaacaacc atgagatcat tacgaccggc
     6121 aacattcgaa atacaatata taaggatgtc ccaaaaaata tagtaaaaaa ctctatcttg
     6181 atagcatcaa taattttcat tactaaaaac agaactgaat aaatcaagat gaaataaata
     6241 catgtcttta ttttacgaaa catcaatact aaaactgtag caataatgaa acaccccact
     6301 tgaaatatat ttccctttcc taaaaaaata atgaccggaa aaaaaatatt taaagtaatc
     6361 aaggttaacg gattgatttt tgacactctt tcacctccaa aaaataagat cgcactgtta
     6421 tcttaagctg ataaaaatgc accgttattc agataacagt gcgatcttat caaaaagcat
     6481 aactgagaac aattctcaat gcaagctttt ttttaattaa ctcagtaatt caatccaccc
     6541 aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt ttgaatagct
     6601 atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt ggatttcttc
     6661 ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat ctagacaaca
     6721 gactgtcttt tttgtcacaa atgatgtaat tttttcaaca atatcttgat atttggttcc
     6781 agctgattgg aataaaacca aatcgaatat acgtttattc tcaaaaatat aattaataaa
     6841 tggatctaat tcattgaagt tctctctcca acttttctct atatctatat tttctaagtt
     6901 taagaggtat ttggtaaact cttcattata catattaata agcgcattaa tatgcggctg
     6961 aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag cacccgtagt
     7021 tacgcctgcg gtcaagcaca gttgccttat cttagtttgc tgaaatccta ctttttcaaa
     7081 atggcttagt ccactttcta ttaatttttg gtgagttgta atataatctc tggtttctat
     7141 gggtacttcc tcctaataag atgaattaac tatattatct tacacaggac ttgaagtttc
     7201 aatcgttaac gtctattact gaactcattc cttagagcta aactaatgag aaagagcaaa
     7261 ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat cattcacctt
     7321 ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg ataagtccta
     7381 tcataatcac tccaatagcc attttcttat cctccctaaa aataaagcct tttacttaaa
     7441 accttttttt gtaatcgtca ataaattttt tccctttatc agttaattga aatcctatat
     7501 ctaaatagtc ctcatatcta agtttagtca gcatctcact tacagatgct ggactaacac
     7561 ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat aattcctgaa
     7621 ttgctagtaa ataaattagt atagtagttg ttttaatttg taccatacct accctccaaa
     7681 ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg catagtaact
     7741 atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat agataggaat
     7801 attggtatct tttgatactg ttttcattgg tctatcgtcc acactgcttt ctacgaataa
     7861 ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat ctggtgttcc
     7921 ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg ctttcgagaa
     7981 atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa ttgaagcaaa
     8041 tttagattta gcttctttgt ctagagaatc caatttttca atatacttat ctagattttc
     8101 tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa tatttttagc
     8161 gtaaataata ccgttttcta aatttaacca agcatgggga tcttccttcc ctttctcact
     8221 ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt ctttgttttc
     8281 ctctttgttc gcatttttta ctaattttgt aaaccaagca tttcctccag tttccaagtt
     8341 aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt caggcaaagg
     8401 ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa ttttatcttt
     8461 tgctatattt tcagtaatat ctgctaaaat cgaattagta actactacct ttaatttttc
     8521 gttcgaagcc actgtatctt tgtctttact atttgttgaa catgccccga atataaaaag
     8581 agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca taactttctc
     8641 tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac aaataagaat
     8701 gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc aatgaacaaa
     8761 cctagcaatg atgagcacgc tccgactgtc gccgaaataa atatcatagt ttttaaactg
     8821 tttgcatata aatatgctgt agcagctggt gtaattaaca ttgcaacaat taatatagtt
     8881 cccacacttt gcatagctgt tactgctact aaagttaata gaatcattaa tagataatga
     8941 taaaaactga cattcatccc cattgctttt gccattaatg gatcaaaaga agtgattaat
     9001 aattctttaa aaaatagaac aactgtaacc aacactaaaa cagaaacgcc aattgtaatc
     9061 cacttatcaa tatcttgtac agctaaaata tttccaaata agatatgaaa taaatccgtt
     9121 gagctatttg caactcctat aaggataact cctaatgcta aaaaagaact gaaagtaata
     9181 ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat aatgatagaa
     9241 gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat ataagaaatt
     9301 gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat tcctcttaaa
     9361 ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt aattagagca
     9421 ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat attattcccc
     9481 ttgtatatta gagttagggt tttaaccttc cggttttgat tggataaccc tatgaaccat
     9541 cgttatttta atttggtatt aactcatttc ttttgagata atatatttat tggattgaga
     9601 ggctaagccg ttcccccctc tccaataaat atagtatctc cataggcttt ttttaaattc
     9661 tcttcattaa atacagattc tacacttccg tgagcaatta gatttcgatt caaaagaata
     9721 atattatcaa aatacttctt taccttgctt aaatcatgat gcacaattag cactgttttt
     9781 ccctgatgtt tcaattcttt taacgtatcc ataattattc gttcactaac taagtctatc
     9841 cctacaaatg gttcatctaa aaatatgaaa tccgcatttt gagcaagaca tcttgctaaa
     9901 agtacacgtt gaaattgtcc tccagataat tctcctattt gatgattaga atattttccc
     9961 atatcgacct tttccaaagc ctttgaaact ctttgccatt ctgcattttt aattctttga
    10021 aaaactttca ttccggcata tgttcccata gatacacatt cttttacttt tattggaaaa
    10081 gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct tcaccagttt tttattgata
    10141 tcttcattat taatggagac ctctccctga tgagggataa tatttaacat tgctttaagc
    10201 aatgtagatt ttccagcacc atttggacca ataatacctg ttaaagcacc ttcttgtatt
    10261 tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac aaacattaag ctttttcact
    10321 tctaacataa ctttctcctt ctaaatgata atgattatca cgatcatatg gtatacaaaa
    10381 attatatttt agtcaaccct aaattttata atttagattt ttttctgttg ttgaagatta
    10441 ggatgtcaaa attatttgaa actactcgta tagtaaatag taagtaattt tcaaaacacg
    10501 aagaaaaaca aaaagaaaaa aggaaacgcc gaagcatttc ctttcagtga tttaatgaga
    10561 acaacacgat ttgacttaga gtctcaaaaa gtcgaatagg tacactcatt taagtgtctc
    10621 cattcgactt tttggggatg agctggctat tgaggtccgc ttaccctatt gaataaatac
    10681 aagattgggt atttctaata agtccgccct tcatattggg gtgatcgggg cgaacatgcc
    10741 atcttttcat ataccacctc ctcgacactc aataatcaaa gattctcgtt aatcttcttc
    10801 gatgcttgca atgatgtttt caatattcat ataacgaaaa tcttcttgtt tgatataaac
    10861 gatctgagcg gtacattttt cctttttgat gatttctttt actttttcct cagaacgtgc
    10921 gacctgcggc gttaaaagta aaacgtcata gtctttataa ttcagttttt cttcaacgtt
    10981 tcggatcgtt gcataatcta tctcatacga tagatttttg gctctgatcg tttcaagcat
    11041 ttttgatacg agcagcgacg cactgattcc ggtctcgcaa ataatcaata gatccttatg
    11101 cataataccc tcctgttttt agcctgcaaa gccaattggt gaaaatgcca cataagttaa
    11161 aataacaatg actaccaatg ctatagcagt caccttggtg tatttccaag gctcgatatc
    11221 gacctttgta tcgaatgagt tcaacacaaa ttctccatca ggtttcaatt tggaaaccac
    11281 catcaaaatg ataatgtcca gaacgaataa tgccgtcaaa acataaagga aatggatatt
    11341 ttggaaaagc atttttgata gtgcatacaa gacgatatgc acggcaatcg tcgtttttgc
    11401 cccaaaagca gttgcttttt ttgaataaaa ggcaaagaga atgatcacga gcagcggcat
    11461 gttgtatagt ccattgaatt cttgaacgaa ctgatacaat ccagccggtg caaaagaaat
    11521 gaatggcgct acgataatgg agataatccc cacaacgacc gttactgttt taccagcacg
    11581 agcgatttgc ttatcatttg ctttcttatt gataagcggt ttataaaaat ccagtgaaaa
    11641 caaggtcgat gtcgcgttca aagctcccgc aaatgatgat aagatcgcgc caaagataac
    11701 agcggcgaaa ataccaaaga tcattttagg caatacttca ctgactaaaa aaggatacgc
    11761 gttatctgga tttgacatta aactatcgcc aaaaatattg cgagcgacga tccctggaaa
    11821 gacaaggaat aacgcaccta agattttgaa gacgccgact aggaaagcgc ccttttgtgc
    11881 ctcagctaaa ttcttgccgg acaatgcttt ttggacgatc atttgattgg tacaccagaa
    11941 atacagattg ttaaacagca tccctgtaaa tagtgtcggc catggaacaa cagccgagtc
    12001 gatcgatccc acagagttaa gcagccatgg cgtgtttacc acaacgtgtt cgatcccgcc
    12061 aacaaagctg ccgtcaccaa gcttcattaa gcctaaaatt gggaccagca gaccacaagc
    12121 cagcagaccg actccataaa tggagtcact attggcactt aacgacaagc cgccgatcag
    12181 cagatagagg atgccgacaa taccaattgc catcgctaca attatgatcg cgacgatcgg
    12241 ctggacgccc agcagctcat caatatggaa gatcttattg aacaccagcg atccagaata
    12301 aagaaccacg ggtaagaaag aagtcatata ggtcacaata aataagatcg aaataattcg
    12361 tttggtaacc gtatcataac gaatctcgat gaaatctgag attgtattga tgccgtattt
    12421 aaaatatttt ggcaagaata tcacagccaa agctacgatc gcaatcgtag cagttacttc
    12481 ccaggccatt acttccatcc ctgcatgata actttgacca ttttgtccga ctatttgttc
    12541 ggtggacagg ttcgtcatga tgatcgtgcc ggcgattggc aaagcagtta agctccgccc
    12601 tcccatgaag aacccttctg atccagaagt atcgacactt ctggatctag aataggcaaa
    12661 taaccataca cagatgacaa tcactaggaa actgattata gaaaaaagat tcatcgtcta
    12721 gctccttttc aatagattaa agaccggtga cctctcgaat gtagcttctg gctttgactg
    12781 catattcaaa tggattggct ttagccgggt cctgttctgc ttcaactacg atccagcctt
    12841 catagtcgct ggcatggatt gctgtccaga ttggagtgaa atcaatcatc ccatcaccag
    12901 gaaccgtgaa gacgcctttc ttcacgccgt ttaagaagct gtctttttcc ttttctactt
    12961 cttcagccgt ttcttgacga atatctttga aatggatgtg tttgatacga tcttgatgac
    13021 ggttgtaaac cgcgattgga ttttcaccag aaaagaccaa atgtcccgta tcgaacaata
    13081 atttaacttt gtctggatca gtcatttcca ttaaacgatc gatttcttcc gtcgtttgaa
    13141 cgccggtacc catgtgatga tggtaaacga tctccatatc cttttcgtgg gccaaatccc
    13201 ctaatttttc taatccttcc gccagttttt tccactcttc atcagtgaaa actggtttat
    13261 catcaaagac tggcgctgtc ataatccctt ggatactatg accctgctca gctactacga
    13321 tgactttagc gcccatcgca tgtaagaaat cccgatgtgc tttgaaggct tcggcagttt
    13381 cttcaaaagg tttagttgtc agaaacgcac tgaaccatgc actggcaacc tttaagccgc
    13441 gaatattcaa atattgttcc aacacctctg gatcttttgg atatttgttg ccaatctctg
    13501 ttccggtgaa cccagcaagg gccatttcac tgatacattg ttcaaaggta ttttcactac
    13561 caagctcggg catatcatca ttggtccacg cgattggtgc gatggctaat tgaatttgtt
    13621 tggtcatatt ctcacactcc tgcttatttt tttagttgta ctaaactctt cgtatcgaat
    13681 gattcttgac aagccaacgc gatcttagtt gattgcaatc catcttctgc attaaccgct
    13741 ggttgacgtt tttcttgaat acatgagaca aattcttggg cttcacgaat gaaggcttct
    13801 ctgaatcgtt ctgggaagtt ttgtgaacat ggacggatga tcccttgatc gttcatgatc
    13861 gtcacaagat ttttctcagg cgcttgcgct acccgcagca ttcctttggt tcccatgatc
    13921 tctgtttcga catgatagcc atgggctgcg tttctgccgg caactagtaa agctattgtt
    13981 ttatcgctca ggctcatcat tgcggctcca gtttcaagct caccagcctg atccaattct
    14041 ggatacgccg catttttccc aatcgcccac acttgatcga cttcttggcc ggtaaaccag
    14101 cgaaccaaat caatatcatg gatcgccata tctaagaaca agccgccgct gtcgttattc
    14161 atggcaaatt tgacaaagct ttccaagcct tcgctcggat cgatcccata acaacgaatc
    14221 agtgtgattt cgccgatttc gccatcttct accattttct tcgcatactg atacgactca
    14281 tcatagcggc gcatgaagcc taacatgaag acctgttcag gatgctcttc gatgactttt
    14341 tgggtctttt taatatcttc aacttcaagg cctaatggtt tttcagtaaa gacatgcttg
    14401 ccattttcca tcgccaagcg gacttggtca cagtgaaagc ctgatggtga aacaataaag
    14461 actgcatcga tcacggaact tttgaccatt tcttcatagc tggaaaaagt atgcttgacg
    14521 cctaatttct tctgtgcgaa tgctaattct gtttcaacta aactgcacgc agccgtaagc
    14581 tcacaacaac tgacacctgt tgctaaattt tgtgcatgta cttttcctaa tcttcctaaa
    14641 ccgacgatgc ctactctaat gttgttcatg aaatacctcc gttttctgag aattactcat
    14701 tgtatagtgc tggtcgttca tccatttgaa tcattacttt tgtttttgtt tcacgagact
    14761 catgacaagc catcatggtg atcgctgcta catacccatc ccaagcggta ggaccgacaa
    14821 ccgcatcatt tttgatcgaa tcgatccaca gcttgaattc ataatcatag gcatccaaaa
    14881 agcgggtctg ccaatctggt gaaacaggtg tgtaattttt ttcttccgat ttaattcgtg
    14941 tataggctgg gtccgtcatt cctacttctg ccgtttcgaa aaccacatca catttaatat
    15001 cgtagccaaa gcggcaattc acaaagattt ccaaatcaat cgttacaccg gattttgttt
    15061 gaaagatcag cagctgcgga tcatgcaaat cggcatggga gttgctggtt tttttcggca
    15121 agatcatttg aacagattca taatcttcat ttagcaacca gcgaagcaca tcaatctcat
    15181 gaatcgcggt gttgctgatc gccatcggtg ttgtgtagtt ttcatctgcg ctaggatttc
    15241 tatgggcaca atgcatcatt aatgcttctc cgtattgctt cgattcgatt gcttctttca
    15301 gttcaatgta gccgcggtca tagcggcgca tgaagccaac ctgaaccaat ttcttcccat
    15361 aagcaacttc cgcatcaacg atccggcggc agcctgctgc atccatcgct aatggctttt
    15421 cgcagaaaac atatttccca tgcttgattg cttctaacac atactgctca tgggtcgggt
    15481 cccaagaagt cacaacaacg acatccacat cttcagaagc gatcaatgct tctccggttt
    15541 caaagaattt agcatcgaca cgtttggcaa cctctttagc agcttttaca ttgatatctg
    15601 agactgctgt cacataagcc ccttgtgttt tctgattgat cctgtcgata tgctcgatgc
    15661 cgattgcacc tgttccgata acgcctactt ttactctcaa ttacttatcc ccctgttctg
    15721 ttgtttaagc acacatagta tgcgcttaca aaattattcc tgtgtagctt ttattcttaa
    15781 aaggtagagc cctctctttg ctccgccttt tactacccat aagcttgtac gtataattcc
    15841 ttaatttgac tatgactagg caccttttta ttattttgcg gacttccgct ggtcaacgca
    15901 tccgcggcca ttttgtctaa ggctgccata aacgcttgct gatcaatacc ataatctcga
    15961 atcgttggga tcgacagctg ctcacagagc tcttggattt ttttaatcag ataatccgca
    16021 gcctcttcca gcgataaatc ttcggctgcc ggataaataa atctggcgat cgtcgctaac
    16081 tcagtctgaa tagcatcctt tgagaattca aaaaagaccg gcaataacat cgcatttgat
    16141 atcccatgag gaatgtgaaa aagtgccccc accggtcgac tcattccatg aatcaatgtg
    16201 actgacgcat tcgaaaaggc aatccccgcc tccgttgaag ctaagctcat ttgttctctg
    16261 gcttcagtgt cgtcgctatt ttcatatgcc tgagaaagcg tgttcaaaat tttcttcatc
    16321 gcttccagtg aaaatagttg cgttacttca ttggctttga ctgaaacata agattccaaa
    16381 gcgtgacata gcgcatcgac tcccgttgcc gcggttactt ttggtggtac agttttcgta
    16441 aaatcagagt caacgattga gatctgtggc atgaaaacga gatccttcat catcattttg
    16501 actgctgttt ttgtattggt aataactgcc acatcggtta cttccgaacc agttccagca
    16561 gttgttgaca ctgcaatcag cggaatgccc gcttgcagct gatcaaaatt tcttgcatga
    16621 atcgcttcga gacttaattg attagcagcc aaaacggcta ccaccttggc tgtatccaga
    16681 cagctgccgc caccaaaagc aatgatatga tcacattgat gcttctgaaa aagcttcagc
    16741 gcatcataaa cattttcttg cgtcggttcg gcttgaatgt ctgtatactc cgctaattga
    16801 tcatctgttt ttcccaacag ctccttgatc ttttgaagat gcccggtctg ttccataacc
    16861 gtatcactaa tgattaacgg cttgttgcct agttgttctg attcttccat aagaaattgg
    16921 attgttccgc cgccaattct taaattggca ggcattttca gttgaatatt cacagaaaaa
    16981 acctctctct ggcttatagc ctataacaga atcctcgaag ctctttaaca gagcttcgaa
    17041 gcaaatcttt aaatctcact ttgcgcataa cgtgctgtaa caactttccg atgggtataa
    17101 aactcaacac tgtctttgcc attggcatgc aaatcaccaa agaaagagtg cttccagcct
    17161 gaaaaggcaa accaagccat cggtgcaggc accccaagat tgacccccag catcccagca
    17221 tcaacatttt ctctgaagta gcgaatcgct gatgcgttgt tggtatacag acacgaacca
    17281 ttcgcaaatt cagattcatt cgtcagctca acggctgcca tcaaatcttt tacccgcacg
    17341 attgatgcga ccggggcaaa aatctcatcc tgccagatct tcatatccat cgtgacatta
    17401 tcaaaaatcg ttggtccaac aaaatagcct ttttctggaa tgttttcgcg accatctagt
    17461 accagtttcg ctccttcatc gacccctttt tggatatagt caaaggtccg ctgctgtgca
    17521 tcttttcgaa tcactggtcc taaaaagacc ccttcgtcca agccattgcc gatcttgatc
    17581 gcttttgccg cagccttaaa tttttcaaca aactgatccg cgatttcctc ttccaaaaca
    17641 atgacggagg ctgccatgca gcgttcacca gcagaaccaa aagctccgcc aataaggtcc
    17701 ttgactgccc gatcaatatt ggcatctttc agcacaacaa catgattttt cgctccagtc
    17761 aacgcttgta cccgttttag attttttgtt ccttcacggt aaacgtattc accgacattt
    17821 tttgatccta caaagctgat gccttttacc agtggatctt gcagcaatgt gttgacaaca
    17881 tcgtgggcac catagacaac attaaaaaca ccgctaggaa agcctgcttt ttcaaccagc
    17941 tcaacaattt tctccatcaa caatggtgtt ttttctgaag gcttcagcac caccgtattt
    18001 cctgccgcca gagccatcgg gaagatccag aagggcacca tcattggaaa attaaacggt
    18061 gtgatccctc cgattactcc gattgggtaa cgataattcg tgatctcaat atcagtcgcc
    18121 gctgttgata aagaatctcc catcattaaa ttggtgattc ccgctgcgtg ctccacattt
    18181 tcgattcctc gttgaacttc tcccagtgac tcggacaatg atttcccatt ttccagcgtg
    18241 atcaaacgcg ccaattcact tttatgatct gttaaaagct gatgcagctt gaacagataa
    18301 cgtccgcgaa caggaacagg aacttttttc caagtttgaa acgcagcatc ggcagcttta
    18361 actgcttcgg ctgtctctgc tttgctagac aacggtacat tcgccagcgc ttcttctgta
    18421 gccggattca ttacttcttc aaactctgtt gagcgactag cgacccaacg accatcaata
    18481 taatttctta aaaatcttgt cattta
//
LOCUS       DAZUMT010000057        18454 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000057 DAZUMT010000000
VERSION     DAZUMT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.057"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(315..398)
                     /locus_tag="ACG8FM_001746"
     tRNA            complement(315..398)
                     /locus_tag="ACG8FM_001746"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="ACG8FM_001747"
     CDS             926..1426
                     /locus_tag="ACG8FM_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5094409.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="ACG8FM_001748"
     CDS             complement(1616..1960)
                     /locus_tag="ACG8FM_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS5094410.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="ACG8FM_001749"
     CDS             complement(2114..3034)
                     /locus_tag="ACG8FM_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5094411.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="ACG8FM_001750"
     CDS             3221..3712
                     /locus_tag="ACG8FM_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS5094412.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="ACG8FM_001751"
     CDS             complement(3736..6111)
                     /locus_tag="ACG8FM_001751"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS5094413.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="ACG8FM_001752"
     CDS             6437..7063
                     /locus_tag="ACG8FM_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5094414.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="ACG8FM_001753"
     CDS             7086..7790
                     /locus_tag="ACG8FM_001753"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5094415.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7982..8815
                     /locus_tag="ACG8FM_001754"
     CDS             7982..8815
                     /locus_tag="ACG8FM_001754"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS5094416.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9012..10940
                     /locus_tag="ACG8FM_001755"
     CDS             9012..10940
                     /locus_tag="ACG8FM_001755"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5094417.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11078..11533
                     /locus_tag="ACG8FM_001756"
     CDS             11078..11533
                     /locus_tag="ACG8FM_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS5094418.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            11613..11741
                     /locus_tag="ACG8FM_001757"
     CDS             11613..11741
                     /locus_tag="ACG8FM_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094419.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            12003..12272
                     /locus_tag="ACG8FM_001758"
     CDS             12003..12272
                     /locus_tag="ACG8FM_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094420.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            12672..12926
                     /gene="rpsT"
                     /locus_tag="ACG8FM_001759"
     CDS             12672..12926
                     /gene="rpsT"
                     /locus_tag="ACG8FM_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS5094421.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13015..14061)
                     /gene="holA"
                     /locus_tag="ACG8FM_001760"
     CDS             complement(13015..14061)
                     /gene="holA"
                     /locus_tag="ACG8FM_001760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS5094422.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14099..16372)
                     /locus_tag="ACG8FM_001761"
     CDS             complement(14099..16372)
                     /locus_tag="ACG8FM_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS5094423.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16365..16904)
                     /locus_tag="ACG8FM_001762"
     CDS             complement(16365..16904)
                     /locus_tag="ACG8FM_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS5094424.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17040..17258)
                     /locus_tag="ACG8FM_001763"
     CDS             complement(17040..17258)
                     /locus_tag="ACG8FM_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS5094425.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17255..17500)
                     /locus_tag="ACG8FM_001764"
     CDS             complement(17255..17500)
                     /locus_tag="ACG8FM_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5094426.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(17616..18344)
                     /locus_tag="ACG8FM_001765"
     CDS             complement(17616..18344)
                     /locus_tag="ACG8FM_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS5094427.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5656 a         3547 c         3228 g         6023 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt
     7861 tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt
     7921 gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt
     7981 tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga
     8041 atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga
     8101 tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc
     8161 tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga
     8221 aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat
     8281 cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc
     8341 cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa
     8401 aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt
     8461 attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc
     8521 tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc
     8581 tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt
     8641 caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt
     8701 cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg
     8761 gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa
     8821 accagaacac ttctcttatt tgagaaatgt tctggttttt tattttcttt aataataatc
     8881 attataaata ataaaaacaa catcaagctt aatcaccaaa tgataaccat tatcgttctc
     8941 atttagctgt tattattatc aattacattt ttattatgct atccttgttt tgacaaggag
     9001 gtctaataac gatgactaat cagaaaaaag cttttgcagc aacaatcggc tgccttttat
     9061 ttatgatctt cggttttatc ttcgaccgat tctctcttac catcacccca cttttttata
     9121 tcagtgccat tatcatcggt ggatataaac aaacatcgga aggcttgaaa gaattgtgga
     9181 atgatcggac actgaatgtg gatttattga tggctttagc tgctattggt gcctgtttga
     9241 tcggtaatta ctttgaagga gcaatgttga cattcatctt ttgtttaagc ggtgcattag
     9301 aggaatacac aactaataaa agtcaaaaag aaattactgc tttgatgaat cttcaaccac
     9361 aaaaagccca attattaaaa gaaaatggac aaatgcaaga agtagatgtt tctcaactaa
     9421 aggtggataa cttggtattt gtcgctaaag gagcagctgt tccgatagat ggctttttag
     9481 aaagcacaca ggcaacgatc gatgagtctg cattaagcgg tgaatctgtg cctgtagaaa
     9541 aatatttagg cgatgagatg tatgcaggaa cgctgaatca aggaaatccc atcacgattc
     9601 gagtaacaaa aacgagttcc gaaactgtct ttgctaagat tatccaatta gtagaagaag
     9661 cgcagaatac gccaactcaa acagcttcat ttatcgaacg aatcgaaaat aattatgtaa
     9721 aattgatttt gcttgctgtt cctctaatga tccttctccc ccactttttc ctaggttgga
     9781 gttgggatga aagcttttat cgaggaatgg tattattagt tgtggcttct ccttgtgcgc
     9841 ttgtcgcatc tgctacacca gcttctctag ctgctctttc aaattctgca aaaaatggga
     9901 ttttaatcaa aggcggtatc catttagaaa agctttctga attaaaagcg attgcctttg
     9961 ataaaacagg aacgctcact aaaggaaaac cagttgtaac tgacagcttt ttctttgatg
    10021 aaaaggattt ggcacaacaa cttcttgttg ctatggaaca aaaaacgacg catccacttg
    10081 ctcatgcgat tttgcaatat ttcgattgca caatacctga agaaatccaa tacttaccaa
    10141 tagaagaaat tactggtttt ggattacaaa ctacctattt aggtgcgcaa tggaaagttg
    10201 gcaagcacgc atatgatcct gagacgatga taatcagcaa agagattgcg gaaatgatcg
    10261 aacgtttgga aaatcaaggc aaaaccgtga tttatctttc taaagatcag cagttaattg
    10321 ctgttttagg tctgctagat attccaaaag caaataccca acaagtaatt tcctacttta
    10381 aatcccaaaa catccataca agtatgataa ccggcgacca ttctggcaca gcaaaggcca
    10441 ttgctgaaca agtagggatt gaacattact atgcaagctg tactccggaa gaaaaaacac
    10501 aactagtcaa aaaagagaat gaactttacc aagtaaatgc gatggttggt gatggagtca
    10561 atgatgcgcc tgctttagca gccgcttctt taggtgtcgc tatggggcaa gggacagatg
    10621 ccgctatgga tatcgcagac atcgtcttaa tgaaaagcga cttaaataaa tttgtctaca
    10681 gtcatcggct atctttaaaa atgcggcgga tcatcaaaca aaatattatt ttttcgattc
    10741 ttgttattct cttgttgatt gcttctaact tcctgcaatt tttaaatcta cctcttggag
    10801 ttgtaggaca cgaaggaagc acaatattgg ttattctgaa tggcttgcgt ttactaagac
    10861 ctttacctga tctttctgat tcttcaaaaa aatgtaagtc ttgtccacta tacaaagcgc
    10921 ggctgtcttc tgtcaaataa gcaaagtgtt tttttatgat tttgtttatt ttatgaaaaa
    10981 aacagcttat gaataggtct tgaaggatga attcctactt ttgtaacttt ctaaaaacat
    11041 gttatgattg tttcgaagaa agtgaggaat atatttcatg gaaagttggc tttttttagg
    11101 aattatttta cttgtagcaa ttttagctaa aaatcaaagt ctgatgatag cggcagcttt
    11161 tgttttgatt atgaagctca taccagcctc ccgttccttt ttcccttggc ttcaaggcaa
    11221 aggaatcaat attggggtga cgatcatcac agcggctatt ctaatcccta tcgcaacagg
    11281 agaaatcggt ctacgtgatc tattagaagc ctttaaatcc cctattggct gggtagctgt
    11341 tttttgcggt gggctagtgg ctgtcttatc ttccaagggt gtaggattga ttgcttctga
    11401 tccacaaatc actgttgcac ttgttttcgg cacgatttta ggtgttgtct tcttaagagg
    11461 aatcgccgcc ggaccggtca ttgcttctgg tatcacttac tgtatcttac aagtatttgc
    11521 tttaggaaaa tagaacaaat tttgtctatt ttaggagatt cttaacaccc ccaatttact
    11581 gatccgtgat atactgacaa cgaggtgata caatgttcga aaaattaaag tatttcatag
    11641 gaacaatcgg ctggtttctt ctagcggtac ttttgattct gggcttagct tatctattat
    11701 ttcctgattt gatacaaaat gtgtttgata ctgctttttg attgacctat taaaacgata
    11761 taggggatgc tcctgcgatg caggggcttt ttttaatcaa tgttttgtaa taatttaggc
    11821 cgtgccaatg agacttccta tagaattcga tgctcttttt tctttttggc tcttctgctc
    11881 ttttttgtta tactagatta cagtggacaa aagaaagaat gatcgcatcc acttcaacga
    11941 atatcaaaat ggaagaatta gtgcctgttt tcaggttact ttgaaaagaa aggaagacgc
    12001 ttgtggaaac ttttgaaggt cttttaattg atacgtacgt tttacaagaa cagccaatga
    12061 tgctgactgg gatcaacttg cagcttgagg accgtgtgat caatggcctg cttaaagatc
    12121 gctatttggc aaaatatatc ttacgtttgc ccctaaataa atatgtgctg gaagtaagtg
    12181 gcaaatggaa ccgtcggaac cagttggtca taaaaaaaat gttgataaaa aatatcgatg
    12241 aatacattaa aataatcggt acaccagaat agcctttact gatcaaagga tactggttat
    12301 aaaataactg cagtttctta atagaaaaac aaagaacaac agggaatcgt atcccttttc
    12361 aaaaaaaact ttgcaaacgg ttctctttgt tatataataa cttagtacca aacattccca
    12421 ttcaaacttt gtttaaatag ataaataaaa atctagtttg gtttgacgct attgcctccc
    12481 acaatagcgt ctttttgatt tttttcagct tgtactatcc gtttttactg aaaaaaaatc
    12541 ctaataaaag actgttaagg aaaaaaactt gtcatgttca tattgcaaag cacgctgcct
    12601 ttaggtataa taatgaatgt tgaattatgc ggtaacacgc gtaagaactt aagttatcgg
    12661 aggtgaaact aatgccaaac attgaatctg caatcaaacg tgttcgtaca agcgaaaacg
    12721 ctaatgtgaa aaattcatct caaacaagcg ctatgcgtac tgcaatcaaa aaatttgaag
    12781 atgctgtagc ttcaggtgct gacaacgtag atgcgttata taaagaagct gttaaagcta
    12841 ttgatatggc tgaatcaaaa ggtctaatcc acaaaaacaa agctaaccgc gacaaatctc
    12901 gtttaagcaa aaaaatcgct aaataaaaga atcggcttcg gccgattttt ttatttgtca
    12961 aaaaaaggac aaaaacaaac gaccttcccg catgctttgc cctcgtcttt cttcttatcg
    13021 atttcttaac aactgccctg tgcgcaaaac gaatagttga aaacttaatt ctttttcgat
    13081 ttgtcccgtt ttgatttgat aatccatctc aactaattga tcaaacaact gtaccagcaa
    13141 atgttcatcg aattttttta cttcctgcat ggcaagtttt acacgatatg gatggatttt
    13201 caatgtttca gcaatatttg cctgctgata accaatcttt acaagaaatt ttgtctgtaa
    13261 aaataaacgt aattgagaaa gcaaaatcgc attaattttg attgtttctt ccccttgcat
    13321 aactaaatct tcgtataaac gcaaagcttg ttccgtcttc ccttttaaaa tatattgggt
    13381 catgtcaaaa atattatgtt ctaacgtttt aggaactaat tcttctactt ctcttaccgt
    13441 gatttgctgt tgattttgtc cataaaggac caatttctgt aactctctca tcattttgga
    13501 taaatctaag tcggtcaacc tcaagaataa atcaattgca cttcgttcca ttttgattga
    13561 ggaattctct aaggtattca tcagatattg acgtacttct ttttcattca actgttggac
    13621 attgatgata attgctgttt ttttcaattg tttcgttact tttttccgtt catccaactt
    13681 ttcatatggc gcaaagaata ctaatacagt cgattctaat ggttctttca aataagccgt
    13741 caataaatca gtattttgtt cgattccatt gtttactttt tcacctgtta aaaaataagg
    13801 attctctacg aatactaacc gttgatctcc gaaaaacggc aacgtctctg cttcagctac
    13861 tacctcatct aaacttgcat tttccatgtc aaaacttaaa aaatttaagt catcttcgcc
    13921 ctctaattgg atttttttga caatttctgt ccgaacttga tcttggagaa acttttctgt
    13981 tcctaaaatc aagtaacaag atgcaagttt ttctttacgt attttttgta actctgcttg
    14041 tatactcaaa ttactcacca ctttcaaaaa ttatcctacc atgagcagat gagaattact
    14101 agtctatcat tgtttccact agcccatgag gatgaaaaac tggatgccag cgataggtga
    14161 tcattccttg ttgatccgtt cgccatgttt ttatctgatt ttcttctagt gtctccaata
    14221 cttcgctgtg cgggtgacca taacgattat ttacgccgca ggatataatc ccatgttttg
    14281 cttcaatatg ggaaagaaaa tctgtgttac ttgacgtacg gctaccgtga tgacctaatt
    14341 tgactacatc ggctttcaga ttaggataaa tcaaaagcaa ttgtttttcc attgcttgat
    14401 tcaaatctcc taaaaataaa aagcgcctat tgttatattc catccataga ccaatcgaat
    14461 cttcatttct tccctctccc ttttcaaaag gagtcaatac ataaagatca taataaccgc
    14521 tgatcctgtc cccatgtttg atttcaatga tctcagtacc atttttttga gcttcagata
    14581 aatgcctttg taatggctca tttcgtccag cgccccaacc gatataaatt tttttgatag
    14641 taaaatgctg agataacgtg ggtaattctc ccacatgatc cgtatcgtcg tgtgtcaaga
    14701 tgagtttatc gattacactt atcccctgac cttttagata tggaacgata tttctttcag
    14761 caggaggacg tatcatacct tgcgcccact tttctctttc aaagtgtaac tgacctccag
    14821 tatcgatcaa aatcgtttct tttcgaaaag gactttgcaa aaagatactg tctccttgac
    14881 cgacatcgat aaaagcgatc gttgaagaaa agcgtaaaaa tggcaaagtc aaaaaaataa
    14941 aagaaaaaag accaacataa aacaagtgtt tcacaggttg ttctttcaac ctatccatac
    15001 aaatcagtaa tccgaaaata tacatcccag atagcacaag gggcaaacgt cctataatga
    15061 gagacgaccc tgaagaaaaa tcgattattc gctcaaataa gattaatccc tcttctatca
    15121 gcaaaagtaa agtttccggc agccagcgat tgaagagtgt gagaaataac acaccaggaa
    15181 gaaaaagaaa cttgaatacc ggtgcaatca gtaatgtaaa aagactacct aaaataggcc
    15241 actcataaaa atgccataca atcaacggca tcgtgatcat agtaaacagg acgtgagagg
    15301 ccgttttttt caaccaatct ccatcaaaag aaatcaggat aaacaaaaaa ctcatgaaaa
    15361 aactaagctg tccacccgca tgaaaaagaa atcgaggatc aaatagtaaa agaagccaaa
    15421 agacaactgc aaataagtcc atggtggaat aatgcttatt cagcaatttg actagcaatc
    15481 gcaataaaaa cagcaaacta gcacgcaaaa cagaaataga acttcctgca atagaaacat
    15541 aacagatagt tagtagactc aacggaataa tactgacgtg tagtggccat ccgatacgtc
    15601 gaaacaaata atacagccac cctaaataaa attggatgtg tagacccgat agactgaaca
    15661 gatgcagcaa accactctcc ctaaaaattt cttctggttc tctgaatcct gcatctttat
    15721 agccccataa taaagaatta atatatacac ttgttttttg cggaaattca gattggacaa
    15781 aggagataaa agatcctctt tttctctgaa gccagtttcg ccagtgattt tgcatggtga
    15841 tctgttcaat cttttgaatt tggaaccgtc cagaaattct atggttcctg aagtaagctt
    15901 cttggtcaaa attataaaga ttccgctttt ttgctggaga gatgaactcg ccttcagcag
    15961 tcaattctat tccctttcct cggaagttaa gccatgtttt caattctgct tctgtgttta
    16021 cttgataaga aagctggagc ttttgtttat tctctgataa ccttccttcc attgtcaaaa
    16081 aattaccatt tgcacgaatc gtatctgtaa atacagtaat ttgcgcagat gttgtttttt
    16141 cctctaaaaa tgaatactct tgtagcctat aagaaatgga taccgttatg ctacacaaaa
    16201 tacacaagta cgcgataaaa cgtccttctt ttagcgaaaa ccataaaaaa attccggcaa
    16261 gaagcaaacg ataaccccaa ttgtcagtgt aatacaacaa acagataatc atcgacgtaa
    16321 ttgccggaaa aatcaaccga tttcgaacat gcctttctaa aacgctagac atgttttcct
    16381 gttaattcat tttgaagttt tgaaaaatgc tgtgagtcta aagcaacttg gtgtacagat
    16441 acacctaatt gttcgatcaa cgcttgggca taaggatcat ttcgatagtc atggagataa
    16501 ttgatccctt tcacaccagc ttgcaacaaa gcctttgtac aggccaaaca tggaaaatgt
    16561 gtgacataga tttctgaacc ttccgtcggg ataccaagtt ttgcacactg taggagtgca
    16621 ttcatctctg catggatcgt tcgcaagcag tgtccatcca cgacgtaaca cccttcatct
    16681 atgcagtgga catctccact gactgctcca ttatagccgc cagcaatgat ccttttttct
    16741 cgaacgagtg tcgctcctac ttctaaacgt gtacacgtac tacgtaaaga taacagtact
    16801 gcctgagcca taaagtactg gtcccaagga atgcgttcat tatgtgactt atcagtaact
    16861 ttttctttat tcgtatcgca tgcttttgaa gatttttcta acatgtttta tattcctccg
    16921 ttttttcaag ttgattaaaa agtactcatt gcttaaacaa aatattcttt aaatataacg
    16981 aatcatcctg tctgacaaaa gatcctaccc ttcactgccg aaatgaagtc cgtcatccat
    17041 tagcccatta taaaaattct tcgaatccat ttgttcaaga aattccgtat aaaaatcgat
    17101 acttcacaat catggcgttt tgctacttct gtgagaacag agatatattc tttgatcaac
    17161 ttgttattcc gatatttttg ttttgtctca tcgactgctg gtggcgtgat aaagaaagca
    17221 tgatcagaac tatagtcttt tttcagctga actatcatcc actgcagatt ttttggtacg
    17281 tttctttaga aagttgttta tgctctgcta gatcattcgt cccaatgaga ataaatactt
    17341 tatctgctgt atagctaagt acatctttgg caaagcgttc tcgtagagct tgactgttat
    17401 tgccgctgac agccgtattc ataatatgaa tcccagatat tttattttgg agatagacat
    17461 tcagcattgg tttttttaat gtttctttgc gaacaatcaa gctatttcct atccaaaaat
    17521 actacctcct cgtcctgcca agctcttaga attggattat tcttagtatc cattagcgtc
    17581 catttattgc tcgtgccagt cgattttgaa tcattctatt taattgtaac gaagtttttt
    17641 agtttttcaa ctgtcttttc accgatacca gaaatctcct gcaggtcttc cactttctgg
    17701 aaggatccgt tttcttctct aaatcgaatg atttcttgcg ctttgactga tccgatccca
    17761 ggaatctgct gaagttctga tggagtggct gtattgatat ttattttggg aggctcggaa
    17821 tctgctgtag gctgttttcc ttccgaggat gccaactgat caagatgttc tggcagctct
    17881 tcacctacag cagggatata taggacttgc tgatcttcta tcttcgcaga aaagttgatt
    17941 cgatcgctat ccgcttcttc taataatcca cctgcacgct ttagtacatc atagacccgt
    18001 tcttctgaag aaaaggaata gataccaggc tccttcacac ttcctttgat atcggtgtac
    18061 atgacctttg gacgagcatc ctcttctaca tcgtttcctt cagctgatgg atcttctcca
    18121 ctggaactaa acgaagaaag tatttctttt gcagcagtct cttctgtttc tctataaaat
    18181 agcaaatttg ataataaaaa accacttgcg atacaaaaga taagaactat catagacatc
    18241 caaatgatca atttgggtga agctagtatt ttcttgctga ttttttctat cattgttctt
    18301 ttttcaaaga atgaccagta aaaatcattc attactcctt tcatttaaaa ttgaagaaac
    18361 agtgagctct ggcaaaatcg tcccttcaac atctaattac gtcaattttt tcttttgttt
    18421 ttatttttat cgtgaagatt tttgacgaaa aaga
//
LOCUS       DAZUMT010000058        18176 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000058 DAZUMT010000000
VERSION     DAZUMT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.058"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..725)
                     /locus_tag="ACG8FM_001766"
     CDS             complement(27..725)
                     /locus_tag="ACG8FM_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS5094428.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="ACG8FM_001767"
     CDS             complement(718..2070)
                     /locus_tag="ACG8FM_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS5094429.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="ACG8FM_001768"
     CDS             complement(2293..3693)
                     /locus_tag="ACG8FM_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5094430.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="ACG8FM_001769"
     CDS             3854..4591
                     /locus_tag="ACG8FM_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS5094431.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="ACG8FM_001770"
     CDS             4716..5195
                     /locus_tag="ACG8FM_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS5094432.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="ACG8FM_001771"
     CDS             5192..5959
                     /locus_tag="ACG8FM_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS5094433.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="ACG8FM_001772"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="ACG8FM_001772"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5094434.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="ACG8FM_001773"
     CDS             complement(6862..7464)
                     /locus_tag="ACG8FM_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5094435.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..7800
                     /locus_tag="ACG8FM_001774"
     CDS             7570..7800
                     /locus_tag="ACG8FM_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094436.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7791..8039
                     /locus_tag="ACG8FM_001775"
     CDS             7791..8039
                     /locus_tag="ACG8FM_001775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5094437.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8145..8729
                     /locus_tag="ACG8FM_001776"
     CDS             8145..8729
                     /locus_tag="ACG8FM_001776"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS5094438.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="ACG8FM_001777"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="ACG8FM_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS5094439.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="ACG8FM_001778"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="ACG8FM_001778"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS5094440.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="ACG8FM_001779"
     CDS             10630..10809
                     /locus_tag="ACG8FM_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094441.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="ACG8FM_001780"
     CDS             10831..11856
                     /locus_tag="ACG8FM_001780"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS5094442.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12047..13291
                     /gene="glyA"
                     /locus_tag="ACG8FM_001781"
     CDS             12047..13291
                     /gene="glyA"
                     /locus_tag="ACG8FM_001781"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS5094443.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13444..14391
                     /locus_tag="ACG8FM_001782"
     CDS             13444..14391
                     /locus_tag="ACG8FM_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS5094444.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14525..15154
                     /gene="upp"
                     /locus_tag="ACG8FM_001783"
     CDS             14525..15154
                     /gene="upp"
                     /locus_tag="ACG8FM_001783"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS5094445.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15293..15535)
                     /locus_tag="ACG8FM_001784"
     CDS             complement(15293..15535)
                     /locus_tag="ACG8FM_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094446.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15656..15728
                     /locus_tag="ACG8FM_001785"
     tRNA            15656..15728
                     /locus_tag="ACG8FM_001785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15689..15691,aa:Thr,seq:cgt)
     gene            complement(15847..16485)
                     /locus_tag="ACG8FM_001786"
     CDS             complement(15847..16485)
                     /locus_tag="ACG8FM_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS5094447.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16608..17240)
                     /locus_tag="ACG8FM_001787"
     CDS             complement(16608..17240)
                     /locus_tag="ACG8FM_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5094448.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17591..17890
                     /locus_tag="ACG8FM_001788"
     CDS             17591..17890
                     /locus_tag="ACG8FM_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5094449.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6245 a         3163 c         3579 g         5189 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg tagattacaa
    12061 aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac aagaacataa
    12121 tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg cacaaggaag
    12181 tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg gtgggtgtga
    12241 atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat tcggagcgaa
    12301 atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt atctcgcttt
    12361 ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac atctaacaca
    12421 tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg gtgttgatcc
    12481 gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac atcaaccaaa
    12541 attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga aattcagaga
    12601 aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg caggattagt
    12661 agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga ctaccactca
    12721 taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag cattagcaaa
    12781 aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc atgtgattgc
    12841 gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat atagtgaaca
    12901 aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg gaaccagagt
    12961 catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt taggtataaa
    13021 tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata aaaactcgat
    13081 tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca cgccagcaat
    13141 cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag ttgtcaaagc
    13201 attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg tacgcgaatt
    13261 aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt aaacagaaaa
    13321 gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga caatatcggt
    13381 gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca gggaggaaaa
    13441 gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga tgcaaatgcg
    13501 cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact agaagaagta
    13561 caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt ggatactggc
    13621 gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga taaattacca
    13681 gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac ccatgataaa
    13741 ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga atatagtcga
    13801 tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt gcaacagctg
    13861 aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc aggagattca
    13921 gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa gggcctccca
    13981 atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac agattcctat
    14041 catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt ttgggaccaa
    14101 tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag agcaaatata
    14161 aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga tgtattaaga
    14221 gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt tacacaagta
    14281 cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca gacaaaggca
    14341 acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata agctttgctt
    14401 gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc gagtttgact
    14461 agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa ggagagctta
    14521 aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac taactattat
    14581 tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa tcgctatgtt
    14641 gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg aaacaccgat
    14701 cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc caatcttgcg
    14761 cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta aagtgggaca
    14821 tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg tcaaacttcc
    14881 agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa caggcggatc
    14941 agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta aatttgtttg
    15001 tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg atattgacat
    15061 ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc ctggactagg
    15121 ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg gtgtgacaca
    15181 acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag ctttttcaca
    15241 gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa attcaaatct
    15301 tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc aagttcgcat
    15361 tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt gaaatcgttc
    15421 cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg aatttttcac
    15481 caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt ttcataaaac
    15541 ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt aaactaattt
    15601 tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg ataaggccag
    15661 cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt cgatccctat
    15721 agctggcatt aaaagagaag acagatatat caaggatttt taatccattg atacatctgt
    15781 ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta ttatttctat
    15841 aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata tcacatgaac
    15901 tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata acaatcttgc
    15961 caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat tatcataagt
    16021 actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag gaatgctgaa
    16081 cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa gtatcatgat
    16141 aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc ctgttaaacc
    16201 ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta cattgataaa
    16261 aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt ttttcgtatc
    16321 ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag aaaaaatgct
    16381 aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacgacaa tagtgcccaa
    16441 tatccctttt ttaagttcta ttttttttcc taaaatgtta ttcatgataa taactcctac
    16501 tattcagttt ttgaagatag agtcagtata catgaagagt taaaataggc aatgtttttt
    16561 tatggatatt tgtattgcta aaaatttatc ttatatattc attttgatta tactactttt
    16621 atttgtaaaa attttctagc aaaaccaagt gcttgcaact gattaagcgt acaatttttt
    16681 agatgttccg agcgtacttc aatcatttca aaggagttgc tacttaaatc gatatttttt
    16741 aaatcagtat caaaaaagct agcattttct aaatcattag tagaaaacat tattttagtg
    16801 agggaacagt ctacaaaatc tgcgcttgat aagacagagt gctcaaaggt actttctttt
    16861 atgtttgaac cagataaatt tacatagtcc attttacaat tctcgaataa cgtgttccca
    16921 atataacttt ccatcaattt agtacctgtt aatttacaat tgacaaattt cacgcgatgc
    16981 aacatgcttt ttgtcaatat acagcctgaa aaatcacagt tttcgaatat acagtcggta
    17041 atctccattc tttgaaaatc attggaagca aaaaatgcat acttgaaaat agatttgtaa
    17101 ataattggat ggcagattat ttggttttcg atgacctctt tttgtgaaag gatattatac
    17161 agttcaaaat cttcgttttc atagatatga aaaaaggatc ctttattcaa gcttgcttca
    17221 ttaactttag gaaaaatcat atttaattac ctccattttc ataaagtcat attaacaaaa
    17281 gctaaaccaa cttacattag cagaaaatga gataggtttt cgcctttttc gataacgata
    17341 gtgtcgctat ctcttgaaac gttcttagaa ttgccctgag agacacaaat taggcgtaat
    17401 tctaataaaa atcttagaat aaaaattttt actgatttaa tttagaatgt ttttaaaata
    17461 aaaatcgttg ccataacagt gtattgaaag gttttaacaa tccagtaatg atttaaaaaa
    17521 atctagatgt agcgtatact ttgtttatag aatgtagtgg gaagaaatat atttttggga
    17581 ggaaatcgtg atgtctaaaa gtgaagaaat agaaaacatg gtactaaaat tatatgatct
    17641 cgttttaaat ccagagatca aagaaaagga acgtatactt ttgatagacg caaagacagg
    17701 cttagaaaaa ggtcagtatt atcctaaagt cataaataat ttggaacgta gtttacgtcc
    17761 gttagcaatc aggggagaat tgtctaaacc agtatcaccg ttttatatgg agatatcaac
    17821 aataggaaaa tttgaaaaag aattaggtcg aggaatggct tcggcaccga ttacttttgg
    17881 gcatttataa aaacgagtta ttcatctcaa tgaattaaaa agtatagtca atctcataat
    17941 gcaggctagg ttcatttatt tgaacagagt ctgtattttt ttattctaaa taaagaaaca
    18001 gatgtttctt tatattgtat tttatccttt aattaacaca aaaacagcta gtataaacta
    18061 ataatgttaa ttaaaagtgt attttatgaa actatcgtta tttattaatg ttgcattttt
    18121 gtaagttaaa aattggaaat atagattgaa aaaatgaaag caataagtaa aataaa
//
LOCUS       DAZUMT010000059        17910 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000059 DAZUMT010000000
VERSION     DAZUMT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.059"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACG8FM_001789"
     CDS             133..636
                     /locus_tag="ACG8FM_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5094450.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACG8FM_001790"
     CDS             763..1800
                     /locus_tag="ACG8FM_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFS5094451.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACG8FM_001791"
     CDS             1815..3512
                     /locus_tag="ACG8FM_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFS5094452.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACG8FM_001792"
     CDS             3505..3966
                     /locus_tag="ACG8FM_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFS5094453.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="ACG8FM_001793"
     CDS             3979..4839
                     /locus_tag="ACG8FM_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094454.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="ACG8FM_001794"
     CDS             4877..5266
                     /locus_tag="ACG8FM_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5094455.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="ACG8FM_001795"
     CDS             5253..7700
                     /locus_tag="ACG8FM_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5094456.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="ACG8FM_001796"
     CDS             7705..9813
                     /locus_tag="ACG8FM_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5094457.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="ACG8FM_001797"
     CDS             9810..10814
                     /locus_tag="ACG8FM_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5094458.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="ACG8FM_001798"
     CDS             10833..11744
                     /locus_tag="ACG8FM_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5094459.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="ACG8FM_001799"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="ACG8FM_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="ACG8FM_001800"
     CDS             complement(12406..12621)
                     /locus_tag="ACG8FM_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094460.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13089)
                     /locus_tag="ACG8FM_001801"
                     /pseudo
     CDS             complement(12880..13089)
                     /locus_tag="ACG8FM_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13392..14831)
                     /locus_tag="ACG8FM_001802"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="ACG8FM_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="ACG8FM_001803"
     CDS             complement(15379..16050)
                     /locus_tag="ACG8FM_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS5094461.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="ACG8FM_001804"
     CDS             complement(16399..17850)
                     /locus_tag="ACG8FM_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5094462.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT         5834 a         3012 c         3466 g         5598 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat
//
LOCUS       DAZUMT010000060        17050 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000060 DAZUMT010000000
VERSION     DAZUMT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.060"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACG8FM_001805"
     CDS             complement(33..1076)
                     /locus_tag="ACG8FM_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5094463.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ACG8FM_001806"
     CDS             complement(1237..1920)
                     /locus_tag="ACG8FM_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFS5094464.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ACG8FM_001807"
     CDS             2115..3044
                     /locus_tag="ACG8FM_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5094465.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ACG8FM_001808"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ACG8FM_001808"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFS5094466.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ACG8FM_001809"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ACG8FM_001809"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS5094467.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ACG8FM_001810"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ACG8FM_001810"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS5094468.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ACG8FM_001811"
     CDS             5429..6232
                     /locus_tag="ACG8FM_001811"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5094469.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ACG8FM_001812"
     CDS             6316..7863
                     /locus_tag="ACG8FM_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS5094470.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ACG8FM_001813"
     CDS             complement(8160..8357)
                     /locus_tag="ACG8FM_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094471.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ACG8FM_001814"
     CDS             complement(8433..8633)
                     /locus_tag="ACG8FM_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5094472.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ACG8FM_001815"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ACG8FM_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="ACG8FM_001816"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="ACG8FM_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5094473.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11515..12297)
                     /locus_tag="ACG8FM_001817"
     CDS             complement(11515..12297)
                     /locus_tag="ACG8FM_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094474.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12362..13198)
                     /locus_tag="ACG8FM_001818"
     CDS             complement(12362..13198)
                     /locus_tag="ACG8FM_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5094475.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13216..14625)
                     /locus_tag="ACG8FM_001819"
     CDS             complement(13216..14625)
                     /locus_tag="ACG8FM_001819"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5094476.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(14639..16462)
                     /locus_tag="ACG8FM_001820"
     CDS             complement(14639..16462)
                     /locus_tag="ACG8FM_001820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5094477.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(16742..16969)
                     /locus_tag="ACG8FM_001821"
     CDS             complement(16742..16969)
                     /locus_tag="ACG8FM_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094478.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         5053 a         3460 c         3191 g         5346 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag tataaaatat
    11281 acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat
    11341 atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg
    11401 ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag
    11461 tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc
    11521 aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga
    11581 ataatctttt ttcccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt
    11641 cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt
    11701 agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt
    11761 aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc
    11821 agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc
    11881 cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata
    11941 ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata
    12001 accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata
    12061 gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga
    12121 ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt
    12181 aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac
    12241 cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga
    12301 atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca
    12361 ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg
    12421 cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa
    12481 aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa
    12541 tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt
    12601 tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc
    12661 ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa
    12721 acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt
    12781 ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca
    12841 ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa
    12901 ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga
    12961 taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat
    13021 ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg
    13081 gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt
    13141 tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta
    13201 gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa
    13261 gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat
    13321 aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg
    13381 taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt
    13441 ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg
    13501 tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct
    13561 caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata
    13621 aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc
    13681 tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata
    13741 attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat
    13801 tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg
    13861 aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc
    13921 atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt
    13981 tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg
    14041 gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat
    14101 cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt
    14161 gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg
    14221 atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac
    14281 aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc
    14341 cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg
    14401 aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa
    14461 atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga
    14521 cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc
    14581 ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt
    14641 aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg
    14701 cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa
    14761 tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta
    14821 caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata
    14881 attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga
    14941 cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc
    15001 ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt
    15061 ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag
    15121 tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg
    15181 taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta
    15241 agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa
    15301 ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga
    15361 gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca
    15421 actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag
    15481 aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag
    15541 cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca
    15601 tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa
    15661 gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta
    15721 aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa
    15781 agggctctac ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat
    15841 aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct
    15901 gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat
    15961 caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag
    16021 caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca
    16081 agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa
    16141 aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg
    16201 ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt
    16261 cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg
    16321 ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg
    16381 tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc
    16441 ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca
    16501 aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt
    16561 aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc
    16621 aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga
    16681 tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta
    16741 actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca
    16801 tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat
    16861 gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga
    16921 tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata
    16981 aaggtatcga tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa
    17041 ctgatttatt
//
LOCUS       DAZUMT010000061        16961 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000061 DAZUMT010000000
VERSION     DAZUMT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.061"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(284..2176)
                     /locus_tag="ACG8FM_001822"
     CDS             complement(284..2176)
                     /locus_tag="ACG8FM_001822"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094479.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2173..3900)
                     /locus_tag="ACG8FM_001823"
     CDS             complement(2173..3900)
                     /locus_tag="ACG8FM_001823"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094480.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3916..4566)
                     /locus_tag="ACG8FM_001824"
     CDS             complement(3916..4566)
                     /locus_tag="ACG8FM_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS5094481.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4770..5066
                     /locus_tag="ACG8FM_001825"
     CDS             4770..5066
                     /locus_tag="ACG8FM_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5094482.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5238..5858
                     /locus_tag="ACG8FM_001826"
     CDS             5238..5858
                     /locus_tag="ACG8FM_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5094483.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5950..7116)
                     /locus_tag="ACG8FM_001827"
     CDS             complement(5950..7116)
                     /locus_tag="ACG8FM_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5094484.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7128..7481)
                     /locus_tag="ACG8FM_001828"
     CDS             complement(7128..7481)
                     /locus_tag="ACG8FM_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5094485.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7609..8631
                     /locus_tag="ACG8FM_001829"
     CDS             7609..8631
                     /locus_tag="ACG8FM_001829"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS5094486.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8650..8811
                     /locus_tag="ACG8FM_001830"
     CDS             8650..8811
                     /locus_tag="ACG8FM_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094487.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9070..9504)
                     /locus_tag="ACG8FM_001831"
     CDS             complement(9070..9504)
                     /locus_tag="ACG8FM_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094488.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9614..11473)
                     /locus_tag="ACG8FM_001832"
     CDS             complement(9614..11473)
                     /locus_tag="ACG8FM_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5094489.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11474..11614
                     /locus_tag="ACG8FM_001833"
     CDS             11474..11614
                     /locus_tag="ACG8FM_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS5094490.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11591..12751)
                     /locus_tag="ACG8FM_001834"
     CDS             complement(11591..12751)
                     /locus_tag="ACG8FM_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5094491.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13058..13969)
                     /locus_tag="ACG8FM_001835"
                     /pseudo
     CDS             complement(13058..13969)
                     /locus_tag="ACG8FM_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(14135..15328)
                     /locus_tag="ACG8FM_001836"
     CDS             complement(14135..15328)
                     /locus_tag="ACG8FM_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5094492.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(15412..15612)
                     /locus_tag="ACG8FM_001837"
     CDS             complement(15412..15612)
                     /locus_tag="ACG8FM_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS5094493.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(16036..16269)
                     /locus_tag="ACG8FM_001838"
     CDS             complement(16036..16269)
                     /locus_tag="ACG8FM_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094494.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(16276..16707)
                     /locus_tag="ACG8FM_001839"
     CDS             complement(16276..16707)
                     /locus_tag="ACG8FM_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5094495.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4770 a         3705 c         3182 g         5304 t
ORIGIN      
        1 aagtaataag aacagccaaa caaaaatagc tcctcctatt tttcgtttgg ttgggcttat
       61 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtaaaat cagcggtttg
      121 acctgagtat tagagataca aaacggaaaa atagcttctc atattttgcc gttttttgag
      181 ctaataccga aggacagctg attgaacacc gtttattcgg aaaaagggac tgtgacacga
      241 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
      301 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
      361 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
      421 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
      481 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
      541 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
      601 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
      661 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
      721 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
      781 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
      841 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
      901 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
      961 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
     1021 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
     1081 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
     1141 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
     1201 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
     1261 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
     1321 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
     1381 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
     1441 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
     1501 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
     1561 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
     1621 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
     1681 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
     1741 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
     1801 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
     1861 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
     1921 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
     1981 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
     2041 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
     2101 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
     2161 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
     2221 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
     2281 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
     2341 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
     2401 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
     2461 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
     2521 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
     2581 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
     2641 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
     2701 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
     2761 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
     2821 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
     2881 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
     2941 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
     3001 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
     3061 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
     3121 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
     3181 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
     3241 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
     3301 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
     3361 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
     3421 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
     3481 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
     3541 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
     3601 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
     3661 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
     3721 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
     3781 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
     3841 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
     3901 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
     3961 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
     4021 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
     4081 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
     4141 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
     4201 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
     4261 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
     4321 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
     4381 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
     4441 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
     4501 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
     4561 cggcatatgg atcctccttt ctttcaagat gacacattgt catatgacgc tgtgtcatta
     4621 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
     4681 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
     4741 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
     4801 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
     4861 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
     4921 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
     4981 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
     5041 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
     5101 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
     5161 ttatcaatat gacttgatag ccttttcttt ttctcatagt tataacaatc atagcaataa
     5221 aaggagaagg taagcatatg gcatctgttt tagtagtcaa aggtcatccc ctaactgcag
     5281 aagaatcgcg cacagttaag gcattgacgt cttttttaac aagttataaa gaaaatcatc
     5341 cagatgatga agtgactgtt ttggaacttt atcgcgatga tattccggaa atcgatgaag
     5401 aattattatc aggttgggaa gcattacgtg ctggtgcaga attcacttcc ttatcagaaa
     5461 gtcaacaaca aaaaattgct cgtttcaatg aattgactga tcagttcctt gctgctgata
     5521 aaatcgttat cgcaaacgcc ttatggaatc taaatatccc aacaaaatta aaagcttggt
     5581 tcgataccgt caatgtagct ggaaaaacgt tccgctatac agaaaacggg ccagaaggat
     5641 tagtgacagg taaaaaagca ctgcacatcc aatctaatgg cggtgtatac aacggacaag
     5701 actttgcctc tcaatatgta aaaggtattt taaactttgt aggcatcgat caagtagatc
     5761 agctattcat cgaaggaatc gattatgacc ctgaccgtgc agatgagttg atgcaaaatg
     5821 cattagataa agctgctgct ttaggtaaat cattttaaaa attgagataa atgcatacaa
     5881 aagagacaga ggaaaaaatc ttcaaagcag catttgtacc ttggatattt tcccatctgt
     5941 ctctttttgt tattctttct gctttttcaa cggattttct tcataaagag aagagattgt
     6001 tgtgtaccat tcaaaaacat gagtaatcac tactttagct gctgcataaa caggaatccc
     6061 caagatcaca ccgatgattc cgaacaattt cccagaagtc agcagaacta ataaaatcgt
     6121 tactggatgg attgctaact gattccctag tactaatgga gagatcaagc gtccttctat
     6181 tgtctgctct actgcaaaaa cgatcagtac tttgatgatc atgaatggac ctgctacaat
     6241 ggctaaaaag atagccggaa tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa
     6301 aaaacccgca ataatcccca acgtgacagc atactctaaa ccaatgaccg caaaaccaat
     6361 cataaacatg attgccacag caaaagccac agttagttgt cctcttatat aagaagaaac
     6421 ttgactattc atttcactca gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa
     6481 tttgacaaaa taaggtgcta attgtttacc gtctttcaat agataaaata agataaacgg
     6541 catcgtcagc agagccacta aaatagtcgc taccgcgccg acaaaacttc caagactctg
     6601 aactgtactc ttagatatat cttggatcat atcgcctgcc gaattcatga acttatcacc
     6661 tgctgtttcc aactgttcgc gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa
     6721 tttattatca atcgtatcta catattgagg gaagttactg atgaagctca ctgtttgttc
     6781 ctgaatctta gggatgatca ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa
     6841 cagcccaatg atactgtaaa gccttggtac ttttcgcttt tcaaagtaat caactaccgg
     6901 attcatcaag taatacaaga ttcctgctaa aatgataggc agacctacga ttgctaaaaa
     6961 ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat aaaaagacga tcagcaatat
     7021 taacaataca acaagtaagg ctgtgactac ttgattattt aaaaaccagc gccaaaacca
     7081 cgataccggt ctgttcttct tattttttcc ttccataatt cctcctgtta gttataaaaa
     7141 atttggcttc ccaaaccaat agcaggcagt ctatgcggat gaagatagat tccatttgca
     7201 atttgtcctt cttctggaca agcatcaaaa atcaatgata cgtgagctac tgtctctaaa
     7261 ttcccattgg ccatcggacc gacataatcg atcgtatata cctgatcatc aaaagcaatt
     7321 gttccgcctg ctttcaaaga aaaaggttcg tcttttgata cttcttgaat caccgaatat
     7381 tttcgtaagg cagctgttgc tgttttatcg aacaaaatca gcatgggttc ttccttgttt
     7441 attgcttctg tcccaatttc tgtaatcttt gcctgaatca ttgcactctc tcctttaatt
     7501 gctttcaaaa acagtataac acattctcat tctttagccg tgtggaataa ttatgtgata
     7561 gaataaaaac gaaaactatt tcattacaat taggaggaat cagctcatat gatcgaaaag
     7621 ataattttag gaacctatac gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat
     7681 acagaaaaag aagaattagc caacctttca ttagctgttg aagaaaatag ccctacctat
     7741 gtagcaaaaa gtaaagctgg caatctttac acagttacta gtgtggatgg attaggtggt
     7801 gccggtgcgt acgataagga ttttcatttt ttgaatgcgg taacagagtc tggcgctcct
     7861 ttgtgttatg tagcggttga tgaagcacgt caattactat acggggcgaa ctatcataaa
     7921 ggtgaagtaa atgtctacca tatattagga gatggcgaac tgaaagcagc agattcaata
     7981 ttccatcaag aagcgacagg tcctcataaa aaccaagatc atgcgcatgt ccactatacg
     8041 gatctgactc ctgaccaacg attagtcgta tgtgatcttg gtacagatcg tgtgtatact
     8101 tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta ccttcactgc agagccagga
     8161 acaggccctc gccatcttgt ctttcatcca aatggacaat tcgcttattt atttggtgaa
     8221 ttagacagca cagtccgcgt gctttcttat aataaagaaa caggtgcttt tgaagaatta
     8281 cagaaaatat cgaccttgcc agaagaattc gacggtgaaa atggaggggc agctatccgc
     8341 atttcaaatg atggcaagtt cctttatgct tctaaccgag gacataactc gattgctgta
     8401 ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc aaatcatctc aacagaaggg
     8461 gattttcccc gcgatttcgc actagatcca agcaataatt ttgtcgtgtg tgccaatcag
     8521 aacaccgata atctgacatt atatcgccgt catcctgaaa caggattact ggaattactg
     8581 aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag
     8641 gagaatgaaa tgaatcagca agacgtatta gacagactac gggaagaact aaatattcct
     8701 ttctttgatg ggaaaatcga agataaagag tatagcgaag aggaatatca gaaactcaaa
     8761 gccgatttac agaactactt tgaacaatat gtacagaatg tagaaaacta atcagctttc
     8821 catactatct gaaaagaaca tcaggacaag acttttccgt ctatcctgat gttcttttct
     8881 acaaataaaa aaccgccgag atggttagac tcgacggaaa aggagtattt actcgatttt
     8941 gagtaagtac aaggttgttg ttggttatag gtatgtcatt atcataaagg attttgatca
     9001 ctctatcata aaggtaatat tacatttata ttagataact ctagttttta ttgttatcaa
     9061 agatttcttt tattttttca acaacaaatt agtcattttt tggatggcag ctgtcaattg
     9121 gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat agatcttcat ctacccctga
     9181 ttcaagatcg atactttgga tcatttcttc acagcctgct acatatgaaa gataagcttt
     9241 ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga cgcaaagtac tgactttctt
     9301 aagcattgcc cgatattttt ttgttccttc ttcaaataac aggatgatct cttcaagagt
     9361 cgatttgtcc agttcacttg tttttccttc atcaattgcg tttctgatca cttcatattt
     9421 cggatgcatt ccttctccga ttgcttctgt ttccttgacg atttgatcga tggactgcac
     9481 ataatagcca atattcggta acatgttcgt tttcctactt tctccattgt tgttattatt
     9541 tattctactt gtttttatcc tcaaatgaca agtacttgaa aaaatttaat ggtttctata
     9601 cagctcgcca ttcttattcg tgctcaaaga aaccatctgc taaactatat tgtttccctg
     9661 cgacaatgtc atattggatc aattgatatt cgtcgtaaat ctcttgttgc gagcgattga
     9721 attgcgcttc tttataccac tgtccgtttt gatagtacag ttctcgttca aacgccggaa
     9781 gttcctgttc taatgataaa agcagctgat aaaagccatt tgttgtctga ttcgtctgtt
     9841 ccattagatt cgctgcaaaa taaaacggac tggtgattgc atcgtgagtc gtttgctttt
     9901 ctaattctcc tttactgtcg aacataagaa attgtgtttc atgcaatgtt tgtttgtcat
     9961 tttttgcttg gatcgtatct gaatagatcc ctggaagatg atccccccaa aaaacaacta
    10021 aagtccttct agaaagattc ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat
    10081 aggaaatatc ttgcagataa ttctctaagt caagtgtccc acttccatct gaagtttttg
    10141 caggatagtc tagattatca tactttccat cgtagggcat atgtgtctgc atcgtgacta
    10201 aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt cattacttct ttgtaagctg
    10261 actcatcaga aatatatgga ttattctcta tcgtatccgt ataggtcatc gtacgctcgc
    10321 tgatgaactg gtcaaatcct aatgtctgat aaacatcctc tcttttatac atcgaagtat
    10381 tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt taacgctgaa acgatagaag
    10441 gaagctgatc catttttggt acaagcatcg tgtaaggggt ggtgagttgc gcattaaata
    10501 acgccataga aaaaccagtc aatgcttcaa attcgatatt tgctgttcca ccgccatagt
    10561 tttgagaaag catattgcca ctatatgttt gatcagctac ttcataataa tctgccaatg
    10621 gctcaccaga tacttctacg ccgtttaatc gactaggatc tgagaaactt tcactcatca
    10681 cgaacacgat gttcggcgac tcttcctcta cagttttatt tttttcatcg gctagcttct
    10741 ggtatttttc agtgatttct ttgatctttt ctttcgaata tccttcaggt tcatccattg
    10801 gttctacctt cagattgtac aaaaacccac cgataaaacc agtgttatag tagttcattt
    10861 tctggctgta aggtatccat agtgccgttt tattatatgc ttttcgcaac aaattatcgg
    10921 gattgttgaa attactgata taccctagca gtccaattgc cacaagcagt gtcgttaatc
    10981 ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa catgtaccag caaaacaatc
    11041 ctatcacggt tcctgcggca gctaagatca ctgtaaacag catagtgccg gtcatatctt
    11101 tcagcaatcc gatttcagtg atcatcttca aatcgtctgg ataaatcggt tcttgccgat
    11161 aactcatttt catataattt gcaaaaccta atacaccgat ggctacgaca tacgtaaggg
    11221 aacctaaagg gacagatcct gctagtgcaa tcagaaaaat aagaataatc agcaagacga
    11281 gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta
    11341 cggatagatc attttgacac cactgcaaaa ataaattact aaaaacaaca agaaaaaaga
    11401 tactgatgat tgctgctgtt ccttttaaaa tctttttctg tttggtcgaa agtctgctta
    11461 tatatctttt caaatgcttg ctccttcatt tctatcaata aaagaatgtt gcttctctta
    11521 ttttagttgt acgaaattcg aaagtaaaga acggaaagaa gaattaatga aatccaaata
    11581 ttctctttct ttatggcctt tctggtttct ttaataagcg gacctttcgt actccagaaa
    11641 ttttttctag ctgttgtttc acttgattga gctgttcaaa atcagtactg tctatgtcaa
    11701 tcaatatgta gacatgctca ttcttatggt tacgcgatat atctgcgatc gctagctcgt
    11761 tctcattcag cagagaaaat atctctgcta atatattggt tttcttttga taaaatatag
    11821 tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg ataattgacc gcttctctaa
    11881 ccgtaccgaa caaaagataa tcttttaagg cttcaactgc caaacgtccg ctgtctgcca
    11941 aagcttcctg agtcgatccc cctagatgag gcaacataag tatacggtca ttttctaaga
    12001 actcttcttc tggaaaatca gtcaagtatt tagccagatg ttgtttttcc aatgcttgca
    12061 taacagcata tttatcaaca atttcacttc ttcctacatt gataagtaca gcatcttttt
    12121 tcattttttc tatattcgcc tgatcgatca atccttttgt atcttctgtc aacggcagca
    12181 tgactacgat gtaatccgag gtagaaagaa ctgtttctaa atctgcttgc cgaacataat
    12241 ccaactgggc atcgcggata gaataaccca agacatccat ccctaagcta tagcaactta
    12301 gcgctacttt tgtgcctatc gctccaagac caagaagacc gatggttttg ccttgcaatt
    12361 ctcgtcccac ataagcactt cttttgtttt ctgcctgttc taaaatatta ggacctgtta
    12421 gtgtttgtac catacgactt gcttcgatta tcggacggga agataagagc aaacagcaca
    12481 aaacaagctc ttttactgca ttagcattta cacctggtgt attcatgacg atcgtcccgt
    12541 tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac accagctctt gaaattgcta
    12601 gcaaatctgg tgtaagccat tcatctttca ctttcgtact acgaataact attccttcgg
    12661 ctggcaaatc atctattatt ttgaaatatt ccccttgtaa cttttctatc tcttgttctg
    12721 gaatagcatc aatcaaatga acgtgataca taaaaccccc tccttactaa caagtatctc
    12781 gaaatttctg caattagaca actgtttacc atttgacttc ctagaagcaa aaagagcagt
    12841 tagagcagag taggctgagt gataaaaata aggctatgac aagttactgc cataagcctt
    12901 attttcgaga gcaaaatatt gagggtgaga tatcttgata aatggtgttc cgttagctga
    12961 cattgataat aagactaaat ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt
    13021 atttcttaaa actaagtggc tttttcacaa cctcttttta tctaataacc actgtttcca
    13081 actcaactaa ttttgcccac gtttcaattt gatctgttgt tagatttagc gagacaacgg
    13141 tatgatgacc gccacctgcc tgaatccatg aatggacacc ttcatggaaa ttcggtttag
    13201 ttttccataa cacacgtgca acaggtaaat taggtgcttc agtcgttggt tcaaaggctt
    13261 ctacttcatt gatcagcagt ttataatgcg taccgaaatc tgccatcgat acgactaccc
    13321 cttctcctgc tttcccatca aatacaagac gcgccggatc ttctctatct cccattgata
    13381 aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt cggatctact tccatcatat
    13441 gggattccaa gattgcttct tggccactag ctagctcgta agtataatct tccatgaatc
    13501 ctgtatcttt ccctcgagcc atgattttca gcaaccgatc aatcgctgca gttttccagt
    13561 cgccttcccc agcaaaaccg tatccttctg ccatcaaacg ttgtacagct agtccaggta
    13621 gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa agcgttatag cctccttctt
    13681 ctaagaaacg gcgaataccg atttcctgct gagcttgtac ttttacatgt tcctcccatg
    13741 tcttttcttc ataatcacca tagtcgaagg tatacaattc cttgtactct tcaaataatt
    13801 gatcgacttc ttcgtctgat acgcgatcga ttacttgtac aagatctccg ataccaaaat
    13861 aatcaacagt ccaaccaaat tgaatctgtg cttccacttt gtctccttct gtgacaccca
    13921 cgtttctcat gttgtcacca aaacgagcca cttttattcc aaaattatat gggattttag
    13981 attatctttt tagaagttct tgaatgtcgg tatagcccgt gtttccgggc ttttccggca
    14041 tttttgatat tgttagatac ttacaaatgt tcttattacc tctcaccatt tccccctcaa
    14101 aagtagtaaa tggagtagta aaaccggcct tgtgtcaggc tgccagccgt tccatttctg
    14161 cccttgcgga atcgaatgtg gcgtgtgcat aatagttcag cgtcatggtg atattggaat
    14221 gtcccataat gtactgcaag gctttggggt tcattcccgc attggctaac cgggtgcaga
    14281 atgtatgacg catagcatgg ggcgtcgtcg ttttcggcaa cgcttcttca tggctcttgt
    14341 tgtacttctt cacaagcctg ccaaacatcc cgccataatc taccgccgtc atgggatagc
    14401 cgttctgttt gagaaacagg aaattggcat acccgtcaat aatgaacggc tttgcacctt
    14461 tgcggttttt tatcactcgc tggaacgcct gatacacttc ctcattcatg gggatttttc
    14521 tgacgccgct ttgggtcttg ggttcgtcaa tatagtaacc gacgccggta tttctcaaaa
    14581 gctggtggct cacgatgatt acccggtttt caaaatccaa atctttgtcc gtcagtccgc
    14641 acagttccga aatgcgaagc cctgtcccca gcagaataac gattgcgtca taatgctttt
    14701 ggtaaacctt atcaccttga ataaaggaca gaaaactttc ttcctgtgct ggtgtaagag
    14761 gcacctttgg ttccgtatcg tcgtccagaa cgtcactaag ctggaagtca aacgggtttt
    14821 ttcggatgca atcgtcctgt atggcggtgt aaaaagccgc tttcagggaa cgcttgtcgt
    14881 tattgatggt cttataagaa aggccctttt ctttcatccg aagcgcccat tcctttgcgt
    14941 cggacagctt cacactgtca atcgggcagc tccccagccg atcttgttcc agaatacgca
    15001 tcagccgttc ccggcccagt ttggtgttgt gcttcacatt gccccggtgc ctgatctgct
    15061 tggcgtagag ctggcagacg gtcattttct tgccgatggt gtctatcccg tcgtcaaggt
    15121 ctttctggat ttctttctcc ttttcccgga gtgaaatatc ctcccgttta cctgccgggg
    15181 tcttatccgt aggcactaac ttccatgagt aaacaaactg cggttttcca aaggtatcaa
    15241 catatttgta agcatatcgt ccgtcttttc tctggctctc tccagaccgc aaaacgcgat
    15301 ttttgctgtc tcgtcttttt tctgacatct cgtcatgctc ctttccatga cggaaagagc
    15361 cgcatatatg cttataaatt attataacat atctgcggcc ctttttcact attagattgt
    15421 gtccaaagtg tcaattattt tctcaaactg cttgcgtttg atctggatgc ggttgccatt
    15481 cataatgacc cagccagagg ccggattttc ctcggccagc ttacgcaatt tgttttctcc
    15541 gatacggaaa tatttagacg cttcctcaat cgtcagcgtg tatttttccc aaataggcac
    15601 atcatggttc ataatgcagc cccctttcac aaattcaaag gtttgtgtga tttaggggca
    15661 tagacggacg gctgctggca cagctccacg ggactttcac ccccgcgtgg ttctcacgcg
    15721 gccctaccca ttgcctgcga cgcttttaac gctcggactg tggctataag ggagtatcat
    15781 agtcctgcct gtacgtcgtc gcccgcaagc cgccacgctc acattgcggc gtggtgttcc
    15841 tcgctccgct tttcccgtag ggaaaagtat catggcgcac cagccgtccg gctcggcaca
    15901 gacagaatgt gtccgtatgc cctatgatgc gccgccgtta tcttgcgggc atatttatca
    15961 aggtgcgatc aggccaccgg gaaaacaccg aaacggggaa agggtaagcc gtttcatgct
    16021 gccggggccg gttcgtcagt tcgtgacgat agcgtggatg agcttgattt ccagacggcg
    16081 cttcatggcc tcgtccagac acacatgcgg atagccgttt tcgtcataga gcgtccgggt
    16141 acacagcttg ttcatgtaac cgctgtaata ctgcaaaacg cgctctacgg ctccggcatc
    16201 cccagcgcgg gcggcctcga tcaccgaaag gggcaaaagc tcaatgccct tgtagttcgg
    16261 gttcttcatc cgcgtctaac ctcccttcgg catcaacgcc tttagctgtt tccgcagctc
    16321 cgataaggta tttgtcctgt gccgctgaac ggcactccgg gacattccca caaggccgcc
    16381 gatctcacca tctgccatat ccagaacaca gtgcaggatt aaaatactct gctccatgct
    16441 gggcagcgcg gcaaaggctt cggcaacaag ctcattgttg atatgtaggt catagccatg
    16501 cgacgaaaac gtaaagctgt cgctggggta gtgatctact gtacagagct tgtccaaatc
    16561 tgcctgtgac aaagagctta gtgacacttc acggtcacgg cggcgcttca tttcagccaa
    16621 gtagcttttg gcctcgttac gcagtaccgt cttgcaaaaa gcatcgaaca tgcaccgcgc
    16681 ttcatagtcg cgggggatag catccatctt ctcaccccct ttctgtgggg gacttaggtg
    16741 gttctctccc tttccgctaa caagactttg acaaggccgc cagctacccg ctaaatacct
    16801 cctttttgaa aatttctcaa aaaatttttt tggggacagc cgggccgcaa aagacgacaa
    16861 aaatcgcccg gcaggtcgca aacctaccgg gcgagaaaat gctttgctat gctgctgatt
    16921 tacatggtat agtacatact cataaccgga atagccctgt g
//
LOCUS       DAZUMT010000062        16894 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000062 DAZUMT010000000
VERSION     DAZUMT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.062"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8FM_001840"
     CDS             complement(451..1761)
                     /locus_tag="ACG8FM_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS5094496.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACG8FM_001841"
     CDS             complement(1773..3191)
                     /locus_tag="ACG8FM_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5094497.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACG8FM_001842"
     CDS             complement(3431..4999)
                     /locus_tag="ACG8FM_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS5094498.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACG8FM_001843"
     CDS             complement(5003..5413)
                     /locus_tag="ACG8FM_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5094499.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACG8FM_001844"
     CDS             complement(5410..6333)
                     /locus_tag="ACG8FM_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS5094500.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACG8FM_001845"
     CDS             complement(6330..7208)
                     /locus_tag="ACG8FM_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS5094501.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACG8FM_001846"
     CDS             complement(7195..8532)
                     /locus_tag="ACG8FM_001846"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS5094502.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACG8FM_001847"
     CDS             complement(8535..9638)
                     /locus_tag="ACG8FM_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5094503.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACG8FM_001848"
     CDS             complement(9712..11220)
                     /locus_tag="ACG8FM_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5094504.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACG8FM_001849"
     CDS             complement(11186..11878)
                     /locus_tag="ACG8FM_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5094505.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACG8FM_001850"
     CDS             complement(11900..12874)
                     /locus_tag="ACG8FM_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5094506.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ACG8FM_001851"
     CDS             13174..14046
                     /locus_tag="ACG8FM_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5094507.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14628..16826)
                     /locus_tag="ACG8FM_001852"
     CDS             complement(14628..16826)
                     /locus_tag="ACG8FM_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094508.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4973 a         3535 c         2740 g         5646 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagctt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattaaacgg cgtttatctt
    14521 gttgattctg cggcaataaa atcagccagc agcaaaacat gaagtgctgt tttttgctgt
    14581 cctcttttat tgcttgaatc agatcacaag aaaaacagcc tcttgattca caaatatttt
    14641 tttcgatatt ctcccggcgt aatcgattcc ttctttttaa agaaacgaat aaaattttgc
    14701 ggatttcgat attgcaatct ttctgcaatc tcttttacgg agaggttagt ctctttcaac
    14761 catttttttg ctacctctaa acgatgattt tgaacaaaat caccaaattt ttctcctgtt
    14821 tcctttttaa atacattact taaatagtta gggttataat gcaacctatc agcgatcaca
    14881 tctaaagaca gatcacgatc atattctgtg tagacgatat gcataatatt ctccgacaaa
    14941 tttttaaatt catgatcagt gattgcttga gagcattctg tgattggcag aactaattgt
    15001 tctaaaacaa gtttttctag ttctttggga tgataattgt tcagtgcttt tacataaatt
    15061 tgtttcatgt tatcaaagtt ttttgaatct actcccaata actgtcctaa ttgaacgagt
    15121 tcattgatca agcggaccga aactacttcc cgactcaaag gatttttatt ttgtgcaaac
    15181 aatgcatcaa tcaattcatg tactaaattc ttcacttcct gatttccaga acgaatggct
    15241 tcaaacaatc gattttgttc ttctatcgga tatctaataa gtgttttttc atgagcagca
    15301 ggaacgattt cctgataaaa aataattgaa cttggaccag tatttacacg ataatacagg
    15361 gcttcttttg cccgatcaac cgattgcttg ctttcattca gcgtttcgaa ccggttactg
    15421 atgcctatac tgatagttaa attcaagtat tcttttacac gtttttgaat gagtcggcaa
    15481 tactccatga cttttttgct tgcttctgca tcatctttat ccattcgata aatagtggct
    15541 tgcatttctt cattcaaaac gatcggcagc attcgatttt cctttggcac aatttccgtg
    15601 atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc caccgtgact atcttctaac
    15661 agatcgattt gaaccaaagc tgtataatag cagtcatttt gagaactata accaaattgc
    15721 tgcaaccgct gatctagttc tcgcctttct actcgatttc taaataaact aagtacaaat
    15781 agcgtttcca gctgtggctt ttgggtaact aagtgtgcac ttaacgcttc tttttctcct
    15841 atgatccgac tgactgattg agcgaccaaa tctagttctt tcccactgaa tccttttgat
    15901 ttaagattca gacgttcctg tatttgagaa atcggacggg caaatctgtc cgaaaataag
    15961 taggaaaaaa gaccaatgag tcctaccagt gttaaagaca ctgcaactag cccgattctc
    16021 agattgcgga tcattgcgcc aattgcacta gagtcaactt ctaatctgta aatccaatta
    16081 ttataatctg atttcagtac tatatacgtt ttatcttttt ctcttatcac tttcagctga
    16141 ttgtaatcta gatccacgtc cgttaataca gttggttgct ctttattttt tactgtctga
    16201 gtagtaaaaa ggttgccaac ttcgttttca atagaaagta tgtcttctcc actttcacca
    16261 acaacttgtt ctattgtatg tttctctatc gtagcaattc ccaatgcata tttttctttt
    16321 tcaaacattg gcaaagaaat caccatttcg attcctttgt ttttcttttg ccaaaataaa
    16381 ttatttttgt tatttatgta cttctgttga tacttttcaa cttcctcttc ggtcagttga
    16441 ctcaacgatc cattgattac tccccattta ccagccaaac tgactaaatc ataattatta
    16501 tcatccataa caatcgtttc tataaaattt agttctttcc tgatttcaga ataagtggag
    16561 taatctgtat atgtcagcgg atgcttgaat atgtccgaga aatttttggt cgaaccatac
    16621 gtagaaaacg aatattcaat cgttctaatt ttccattcaa cgttagtttt aacttgcctg
    16681 agcaaattct ttttgtctgt taaataactc gattcgatcg attcataggt attctggtaa
    16741 atgaataaac taaatcctat gacaagtacg gctcctaata aaaacatcgg taaaaatatt
    16801 ttataaaata ctgtactttt cttcattttc ttcctctcct ccttttaaaa taaaaggctt
    16861 tcatttttct gtaaaattga tactcatcta atta
//
LOCUS       DAZUMT010000063        16090 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000063 DAZUMT010000000
VERSION     DAZUMT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.063"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8FM_001853"
     CDS             226..894
                     /locus_tag="ACG8FM_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS5094509.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG8FM_001854"
     CDS             complement(1094..1414)
                     /locus_tag="ACG8FM_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094510.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG8FM_001855"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8FM_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5094511.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8FM_001856"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8FM_001856"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS5094512.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8FM_001857"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8FM_001857"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5094513.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACG8FM_001858"
     CDS             complement(5260..6684)
                     /locus_tag="ACG8FM_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS5094514.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACG8FM_001859"
     CDS             6859..7764
                     /locus_tag="ACG8FM_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS5094515.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8FM_001860"
     CDS             7855..8532
                     /locus_tag="ACG8FM_001860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5094516.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8FM_001861"
     CDS             8529..8699
                     /locus_tag="ACG8FM_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094517.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG8FM_001862"
     CDS             8813..9709
                     /locus_tag="ACG8FM_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5094518.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG8FM_001863"
     CDS             complement(9764..11134)
                     /locus_tag="ACG8FM_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5094519.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG8FM_001864"
     CDS             11372..12118
                     /locus_tag="ACG8FM_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5094520.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG8FM_001865"
     CDS             12115..12954
                     /locus_tag="ACG8FM_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5094521.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG8FM_001866"
     CDS             12955..13905
                     /locus_tag="ACG8FM_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5094522.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG8FM_001867"
     CDS             14046..14798
                     /locus_tag="ACG8FM_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094523.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG8FM_001868"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG8FM_001868"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS5094524.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5220 a         3003 c         3233 g         4634 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt
//
LOCUS       DAZUMT010000064        15900 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000064 DAZUMT010000000
VERSION     DAZUMT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.064"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACG8FM_001869"
     CDS             complement(52..522)
                     /locus_tag="ACG8FM_001869"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS5094525.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACG8FM_001870"
     CDS             complement(681..1139)
                     /locus_tag="ACG8FM_001870"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS5094526.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACG8FM_001871"
     CDS             complement(1310..2455)
                     /locus_tag="ACG8FM_001871"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS5094527.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACG8FM_001872"
     CDS             complement(2468..3385)
                     /locus_tag="ACG8FM_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS5094528.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACG8FM_001873"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACG8FM_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS5094529.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="ACG8FM_001874"
     CDS             4477..4647
                     /locus_tag="ACG8FM_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094530.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="ACG8FM_001875"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="ACG8FM_001875"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS5094531.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="ACG8FM_001876"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="ACG8FM_001876"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS5094532.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /gene="dapA"
                     /locus_tag="ACG8FM_001877"
     CDS             6911..7792
                     /gene="dapA"
                     /locus_tag="ACG8FM_001877"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS5094533.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /gene="dapB"
                     /locus_tag="ACG8FM_001878"
     CDS             7789..8505
                     /gene="dapB"
                     /locus_tag="ACG8FM_001878"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS5094534.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="ACG8FM_001879"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="ACG8FM_001879"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS5094535.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="ACG8FM_001880"
     CDS             9222..10223
                     /locus_tag="ACG8FM_001880"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS5094536.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="ACG8FM_001881"
     CDS             10220..11581
                     /locus_tag="ACG8FM_001881"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS5094537.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="ACG8FM_001882"
     CDS             11541..12692
                     /locus_tag="ACG8FM_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5094538.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="ACG8FM_001883"
     CDS             12667..13836
                     /locus_tag="ACG8FM_001883"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5094539.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="ACG8FM_001884"
     CDS             14011..15390
                     /locus_tag="ACG8FM_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5094540.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="ACG8FM_001885"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="ACG8FM_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5202 a         2803 c         3384 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAZUMT010000065        15888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000065 DAZUMT010000000
VERSION     DAZUMT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.065"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8FM_001886"
     CDS             complement(59..187)
                     /locus_tag="ACG8FM_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5094541.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8FM_001887"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8FM_001887"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5094542.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8FM_001888"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8FM_001888"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5094543.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8FM_001889"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8FM_001889"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS5094544.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8FM_001890"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8FM_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS5094545.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8FM_001891"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8FM_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS5094546.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8FM_001892"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8FM_001892"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS5094547.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8FM_001893"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8FM_001893"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS5094548.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8FM_001894"
     CDS             10491..12050
                     /locus_tag="ACG8FM_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS5094549.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8FM_001895"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8FM_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5094550.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8FM_001896"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8FM_001896"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5094551.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8FM_001897"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8FM_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5094552.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         4104 a         3483 c         2903 g         5398 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtc
//
LOCUS       DAZUMT010000066        15255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000066 DAZUMT010000000
VERSION     DAZUMT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.066"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="ACG8FM_001898"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="ACG8FM_001898"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5094553.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /gene="rpoZ"
                     /locus_tag="ACG8FM_001899"
     CDS             952..1257
                     /gene="rpoZ"
                     /locus_tag="ACG8FM_001899"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5094554.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="ACG8FM_001900"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="ACG8FM_001900"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS5094555.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="ACG8FM_001901"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="ACG8FM_001901"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5094556.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /gene="fmt"
                     /locus_tag="ACG8FM_001902"
     CDS             4417..5355
                     /gene="fmt"
                     /locus_tag="ACG8FM_001902"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS5094557.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="ACG8FM_001903"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="ACG8FM_001903"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS5094558.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="ACG8FM_001904"
     CDS             6726..7466
                     /locus_tag="ACG8FM_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS5094559.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="ACG8FM_001905"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="ACG8FM_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS5094560.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="ACG8FM_001906"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="ACG8FM_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS5094561.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /gene="rpe"
                     /locus_tag="ACG8FM_001907"
     CDS             10609..11259
                     /gene="rpe"
                     /locus_tag="ACG8FM_001907"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5094562.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="ACG8FM_001908"
     CDS             11260..11898
                     /locus_tag="ACG8FM_001908"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS5094563.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="ACG8FM_001909"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="ACG8FM_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS5094564.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="ACG8FM_001910"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="ACG8FM_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS5094565.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..15249
                     /locus_tag="ACG8FM_001911"
     CDS             13675..15249
                     /locus_tag="ACG8FM_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS5094566.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5114 a         2864 c         3319 g         3958 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc aacagaagaa
    14641 tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca
    14701 gcaacagaag aatcaacaac agtgcctgaa tcttcaacaa cagaagaatc aacaacacct
    14761 gcgcctacaa caccatcaac agatcaaagt gttgatacag gaaacggcac aggaagtagt
    14821 actccggctc caacgccaac accaacacct gaacaaccaa aacctgtaac acctgctcca
    14881 gctccttctg gaagtgtgaa tggtgcagca atcgtagcag aagcttacaa atatattggt
    14941 actccatatg tttggggcgg taaagatcca agtggctttg actgctcagg gttcacacgc
    15001 tatgtttaca tgcaagtaac tggtcgtgac atcggtggtt ggacagttcc tcaagaatca
    15061 gctggaacaa aaatttcagt ttctcaagca aaagctggag acttgttatt ctgggggtca
    15121 caaggcggaa cttaccacgt agcgattgct ttaggcggag gacaatatat ccatgctcct
    15181 caaccaggcg aaagcgtaaa agttggttca gttcaatggt ttgcccctga ctttgctgtc
    15241 agcatgtaag aatga
//
LOCUS       DAZUMT010000067        15137 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000067 DAZUMT010000000
VERSION     DAZUMT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.067"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG8FM_001912"
     CDS             4..2376
                     /locus_tag="ACG8FM_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS5094567.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG8FM_001913"
     CDS             2437..3897
                     /locus_tag="ACG8FM_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS5094568.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG8FM_001914"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG8FM_001914"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5094569.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACG8FM_001915"
     CDS             6128..6277
                     /locus_tag="ACG8FM_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094570.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACG8FM_001916"
     CDS             6352..6945
                     /locus_tag="ACG8FM_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS5094571.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACG8FM_001917"
     CDS             6958..7857
                     /locus_tag="ACG8FM_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS5094572.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACG8FM_001918"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACG8FM_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5094573.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACG8FM_001919"
     CDS             8723..8983
                     /locus_tag="ACG8FM_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094574.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACG8FM_001920"
     CDS             9428..9634
                     /locus_tag="ACG8FM_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS5094575.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACG8FM_001921"
     CDS             9655..9885
                     /locus_tag="ACG8FM_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094576.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACG8FM_001922"
     CDS             9901..10338
                     /locus_tag="ACG8FM_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094577.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACG8FM_001923"
     CDS             10331..11038
                     /locus_tag="ACG8FM_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094578.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACG8FM_001924"
     CDS             11202..11402
                     /locus_tag="ACG8FM_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094579.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACG8FM_001925"
     CDS             11418..11597
                     /locus_tag="ACG8FM_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094580.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACG8FM_001926"
     CDS             11922..12185
                     /locus_tag="ACG8FM_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFS5094581.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACG8FM_001927"
     CDS             12179..12529
                     /locus_tag="ACG8FM_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5094582.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACG8FM_001928"
     CDS             complement(12553..13167)
                     /locus_tag="ACG8FM_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5094583.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="ACG8FM_001929"
     CDS             13481..13903
                     /locus_tag="ACG8FM_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094584.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="ACG8FM_001930"
     CDS             13913..14116
                     /locus_tag="ACG8FM_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094585.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="ACG8FM_001931"
     CDS             14327..14911
                     /locus_tag="ACG8FM_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5094586.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5570 a         2450 c         3060 g         4057 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc actcatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
    15001 tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
    15061 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
    15121 ttcaaattaa ttagtaa
//
LOCUS       DAZUMT010000068        15126 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000068 DAZUMT010000000
VERSION     DAZUMT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.068"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(501..1067)
                     /locus_tag="ACG8FM_001932"
     CDS             complement(501..1067)
                     /locus_tag="ACG8FM_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5094587.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1067..2023)
                     /locus_tag="ACG8FM_001933"
     CDS             complement(1067..2023)
                     /locus_tag="ACG8FM_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS5094588.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2023..2679)
                     /locus_tag="ACG8FM_001934"
     CDS             complement(2023..2679)
                     /locus_tag="ACG8FM_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5094589.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3049..5067
                     /locus_tag="ACG8FM_001935"
     CDS             3049..5067
                     /locus_tag="ACG8FM_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5094590.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5137..5343
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5494..7908
                     /gene="leuS"
                     /locus_tag="ACG8FM_001936"
     CDS             5494..7908
                     /gene="leuS"
                     /locus_tag="ACG8FM_001936"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS5094591.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8256..9809
                     /locus_tag="ACG8FM_001937"
     CDS             8256..9809
                     /locus_tag="ACG8FM_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS5094592.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10190..11974)
                     /locus_tag="ACG8FM_001938"
     CDS             complement(10190..11974)
                     /locus_tag="ACG8FM_001938"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094593.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11989..13710)
                     /locus_tag="ACG8FM_001939"
     CDS             complement(11989..13710)
                     /locus_tag="ACG8FM_001939"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094594.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13810..14571
                     /locus_tag="ACG8FM_001940"
     CDS             13810..14571
                     /locus_tag="ACG8FM_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5094595.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4933 a         2772 c         2979 g         4442 t
ORIGIN      
        1 ctgctttgat cataaaagtc tgcatattca tcactgatat tttttctaat aaattcttcc
       61 atcataaaaa tcacctcaaa acgattatac gaacttacgt tcttgttgta aagcgatatt
      121 tgaggggcaa aaaaggggca taatttgact gatattaagt tagctattga tatatgaatg
      181 acatacaaaa atataaaatc ttttaaaaat gcttatatat caatgttttt gccattcttt
      241 tatatatctg ttatatgtca aaaaaatgtg ttcaatttca atttgtattt gaataaagag
      301 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caatcaaaaa cagcttctca
      361 tgtttttgat ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa
      421 gaggttatga aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat
      481 ttatgttttg cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg
      541 ttcttttggt tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa
      601 caaaaagaat caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca
      661 aggatcaatc gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta
      721 gtctctggca tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc
      781 cctgctttga gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga
      841 aacaatgaga cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt
      901 gcttgttgct ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga
      961 aaaaccgtat cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag
     1021 atttcttgca gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct
     1081 ttttccgtac atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg
     1141 cattcaagac ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg
     1201 catcccctgt caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa
     1261 tgacattgac atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca
     1321 acattctcgt tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat
     1381 ctaaaatggt gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt
     1441 tgatcaaatg cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat
     1501 aagtttgata atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca
     1561 atccaagttc tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt
     1621 catctggcag acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga
     1681 ctacttgctc aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt
     1741 ggaaataaac gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt
     1801 tctggaattg gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata
     1861 cactgcaaaa agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc
     1921 ctccatcgat cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc
     1981 aagaatgata tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct
     2041 gtaagagtta tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca
     2101 agaaagactg agtaagtagc cgccgatgat atcagtagga aaatgaacgc ccagataaat
     2161 gcggcttatc ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa
     2221 ggttcttttt tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact
     2281 gcctgctgca tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga
     2341 aggacgctct cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat
     2401 aaatccagaa acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc
     2461 cgcacaagca aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg
     2521 gtagctgttc agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc
     2581 agaaagccac gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa
     2641 gaagctacca gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa
     2701 tgacttatta tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta
     2761 tgattcctct ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat
     2821 tttttggtaa tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt
     2881 aaacaagaac gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa
     2941 attacaatat gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga
     3001 gaagttataa ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt
     3061 gcaagaaaag aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg
     3121 gcagcaatcg tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg
     3181 gttcctgttc aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca
     3241 tttatcacag aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca
     3301 tcagtgatga tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa
     3361 gcaccttatt ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg
     3421 gatacgcttg aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat
     3481 ccttcttttg ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc
     3541 aacggttatt actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca
     3601 gcttgtttga cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc
     3661 atcgcaactt acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga
     3721 gcgactacag gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct
     3781 ggtaatacaa actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct
     3841 gtttggggaa tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac
     3901 atccaaaaca atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat
     3961 ggctcttcga cgaataattc cggtaacaat accaactctt caggaaatgc tggtacatca
     4021 aacggaggac aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag
     4081 atctcaaatg atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac
     4141 aatttcgttt atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga
     4201 gcaaatactg gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct
     4261 tctaatacag cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc
     4321 tcaaataaat acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac
     4381 tttatctacc caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca
     4441 ggtaatacag gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt
     4501 tcgtctcaag gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa
     4561 aacggcatca cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat
     4621 ccaggacagc aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact
     4681 acttctgcag cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact
     4741 ggtaatacga tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat
     4801 catatcacga tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca
     4861 ggacagcaag tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact
     4921 ggttcgaaaa cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat
     4981 ggaattacaa tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca
     5041 ggtcaaacgt tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc
     5101 acaaaaatat ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt
     5161 aaaaagactg aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa
     5221 ctcgcctttg agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta
     5281 caacgttcga ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta
     5341 taaaaagagc aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa
     5401 agcaaaagga aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa
     5461 acaagtgttt tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag
     5521 aaaaaatggc aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa
     5581 aagccaaaat tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta
     5641 gggcatcctg aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga
     5701 tacaacgtgc tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg
     5761 ctagatacag gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc
     5821 caaatcaatt ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg
     5881 gagtattata aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat
     5941 gaagcagaag tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa
     6001 gtgatcgatg ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa
     6061 tggatgctga aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat
     6121 tggccagaaa gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat
     6181 attgaattta aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat
     6241 actctattcg gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt
     6301 acgacccctg aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca
     6361 gatctaaaga gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca
     6421 atcaatccag ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca
     6481 tacggcacag gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct
     6541 caaactttcg gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct
     6601 tacacagaag atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa
     6661 gcaatcgata aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc
     6721 tatcgcttac gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc
     6781 atccattggg aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg
     6841 cctaagacaa aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag
     6901 gagtgggtca atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca
     6961 atgccacaat gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa
     7021 aaagaactag cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt
     7081 ggagcagaac atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac
     7141 gatatcggtg ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc
     7201 ttaggcgaaa acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat
     7261 gtggtcgaaa cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta
     7321 gatgcttcga ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt
     7381 gtatggcgtt tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat
     7441 gatggcaagc ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa
     7501 aatcttcatt tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa
     7561 gtggacgctc tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct
     7621 cctcacatgg gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca
     7681 ccatggccaa catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa
     7741 gtaaacggta aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta
     7801 gaaaaagcag cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga
     7861 aaagttattg cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt
     7921 caataaggac tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc
     7981 tcagattttt tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat
     8041 gttctcaaac acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat
     8101 catcaaattt ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag
     8161 agtacatacc aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac
     8221 gcataatgaa tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg
     8281 aagtattatc agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa
     8341 tgttcaagct tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt
     8401 tcctatatcg ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata
     8461 atttgtatca cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc
     8521 ttctacttat ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc
     8581 aaagagcaca acagttttta cataataaac caacttctca agatgatgac tatctttatt
     8641 ttgagaagac ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc
     8701 tgagatcact tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga
     8761 acacattcca tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc
     8821 ctggtggata cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt
     8881 tagctggaac aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg
     8941 ctgcttctca catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag
     9001 ggttgttagc tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca
     9061 aacttgatac agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat
     9121 ttgtgccgat tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga
     9181 ttcctgaagg ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta
     9241 tctattcttc tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc
     9301 aaacgtctat tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg
     9361 gcaacgatgg tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca
     9421 tttacactac tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc
     9481 agccagtaca aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa
     9541 gtttgtttta tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg
     9601 aaaactatca gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa
     9661 gcgcgaatac gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga
     9721 aaatgtttgc ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc
     9781 aactatcgca agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca
     9841 ccgagtcaca agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc
     9901 gtgattcgga gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt
     9961 acaagatcgt gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg
    10021 gattgtgaca agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag
    10081 tatcattagt attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat
    10141 cgatcatgga ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct
    10201 gaaattgact attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg
    10261 ttcctttttc gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg
    10321 ttgacaaacg gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt
    10381 gctgaatcaa tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga
    10441 caggaggatt cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata
    10501 acgtgccgtt ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt
    10561 gatcacattg tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc
    10621 cgtaggcgat attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc
    10681 caaaagaacg acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga
    10741 tctttccttt attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg
    10801 caccagttgg accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat
    10861 tcatcaatat tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt
    10921 tcggttgttc taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata
    10981 tctcaaatat cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag
    11041 cggtagaaat tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga
    11101 tcgtcacacc acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga
    11161 gttgattaat gaaacgaatg gccggataga tagcacagtt cataaattga gcacgtttaa
    11221 aagcgttgta ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat
    11281 tgaatgtttt gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac
    11341 ccagttcata ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa
    11401 caattgaact gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa
    11461 acagcatcag accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg
    11521 tcaataacac ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta
    11581 cttgatgatt gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc
    11641 gcaccctcaa tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag
    11701 tgatgctgct taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt
    11761 tgatatcact gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat
    11821 cgatcccgat tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc
    11881 atagaaacaa tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca
    11941 aagcacgcgt gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg
    12001 tgattttgca aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg
    12061 tccaacgatt tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag
    12121 gcgttgagca acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa
    12181 tgctgcatct gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc
    12241 tggtttgatc aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga
    12301 accgccttga gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa
    12361 ttgagcgatc ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg
    12421 caagttgctt aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg
    12481 ggagcggatc tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc
    12541 tatcgtaggg tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt
    12601 cccgccaacg atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac
    12661 tggatcagag atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg
    12721 atcaaaggtt ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg
    12781 cagtatttct tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat
    12841 agctgcgatc atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac
    12901 accgatttgt aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata
    12961 aacgattccc atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt
    13021 gatcatttga ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg
    13081 attatcaaag gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat
    13141 ccgatccatt tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata
    13201 aacaatccat acataaaaaa caacagagat caatgtaacc catgttagta atagagaata
    13261 acgaaacatc atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca
    13321 aaataaaaca atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga
    13381 tccagaacta aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt
    13441 ctcccgcgtt tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac
    13501 tgcagcgatt gttcctagaa acgctacaag cagcatttca actccaattt tttttataac
    13561 tgaggcttcg ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc
    13621 taatgtaccg gcgatttcta aacagatcat cacaaaggta aggagagcga gtcctttgtt
    13681 tttctgtaag aatgttttta agatagacat attattcctc cttttttcct gatacttttc
    13741 gattctatac cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg
    13801 tgagaaaaaa tgaaaaaaat gtatacgatt ggccaagtat cggaactatt tgatttgcca
    13861 aaatcaacga tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag
    13921 aatgattatc gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat
    13981 agaaaattag atataccgat caaacagatg aagaatttat atggaaaaac attgacggag
    14041 ttatatgaga cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa
    14101 caaaaacaaa aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac
    14161 gaggaagaat ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa
    14221 gatattgtgg aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg
    14281 ccagcttcca gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta
    14341 tttactcaag acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg
    14401 aaatccgaga tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat
    14461 gaaaaaggat ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tacggatgaa
    14521 aacaatatca gaactgaata ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt
    14581 gtttaagata actcgaaaat gatggttgtt ttagttataa agaaaaattt tctggttcga
    14641 ttacgggttt aatgtggggg attattagtc gtggatagat agaattttag ttttttagag
    14701 gaaatatgtc tttaaaaatg gaatggttgc aattcttagc gatatatacg atgtaattgt
    14761 cctcttaaaa aagtatggaa aaataattgt attgggtgtt atgtataatt catggtgaac
    14821 tgagactaat gagaagaaaa attcatattg ttatgagtga gattgtatca gatgagaatg
    14881 ataaattaaa taggtgcata aaagcattac acttaaaata cattgttgct tacattatta
    14941 caatatgtta ataaggcaaa aataaataga tggattgaat aaacatattt tttaatcaaa
    15001 tagtccctac tgtgactttc ctcagctatc ggtgatacta ccgatagcat ttttttgctt
    15061 ataaaataat tataagtatt tagagatttt tcttctaaca ttcttcaaac aagaaaaatc
    15121 acaaag
//
LOCUS       DAZUMT010000069        14994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000069 DAZUMT010000000
VERSION     DAZUMT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.069"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG8FM_001941"
     CDS             <1..608
                     /locus_tag="ACG8FM_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094596.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="ACG8FM_001942"
     CDS             628..1656
                     /locus_tag="ACG8FM_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5094597.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="ACG8FM_001943"
     CDS             1653..2411
                     /locus_tag="ACG8FM_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5094598.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="ACG8FM_001944"
     CDS             complement(2497..3237)
                     /locus_tag="ACG8FM_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5094599.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="ACG8FM_001945"
     CDS             3298..4551
                     /locus_tag="ACG8FM_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5094600.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4846..5100
                     /locus_tag="ACG8FM_001946"
                     /pseudo
     CDS             4846..5100
                     /locus_tag="ACG8FM_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="ACG8FM_001947"
     CDS             5222..5548
                     /locus_tag="ACG8FM_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5094601.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="ACG8FM_001948"
     CDS             5616..6329
                     /locus_tag="ACG8FM_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5094602.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /gene="licT"
                     /locus_tag="ACG8FM_001949"
                     /pseudo
     CDS             6639..7491
                     /gene="licT"
                     /locus_tag="ACG8FM_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7612..9477
                     /locus_tag="ACG8FM_001950"
     CDS             7612..9477
                     /locus_tag="ACG8FM_001950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5094603.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9491..10934
                     /locus_tag="ACG8FM_001951"
                     /pseudo
     CDS             9491..10934
                     /locus_tag="ACG8FM_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11042..11743
                     /locus_tag="ACG8FM_001952"
     CDS             11042..11743
                     /locus_tag="ACG8FM_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5094604.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11869..12381
                     /locus_tag="ACG8FM_001953"
                     /pseudo
     CDS             11869..12381
                     /locus_tag="ACG8FM_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12779..12988)
                     /locus_tag="ACG8FM_001954"
     CDS             complement(12779..12988)
                     /locus_tag="ACG8FM_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS5094605.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13229..13804
                     /locus_tag="ACG8FM_001955"
     CDS             13229..13804
                     /locus_tag="ACG8FM_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS5094606.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13804..14244
                     /locus_tag="ACG8FM_001956"
     CDS             13804..14244
                     /locus_tag="ACG8FM_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5094607.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14260..14961
                     /locus_tag="ACG8FM_001957"
     CDS             14260..14961
                     /locus_tag="ACG8FM_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5094608.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5066 a         2485 c         3161 g         4282 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
    13141 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
    13201 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
    13261 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
    13321 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
    13381 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
    13441 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
    13501 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
    13561 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
    13621 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
    13681 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
    13741 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
    13801 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
    13861 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
    13921 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
    13981 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
    14041 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
    14101 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
    14161 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
    14221 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
    14281 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
    14341 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
    14401 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    14461 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    14521 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    14581 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    14641 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    14701 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    14761 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    14821 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    14881 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    14941 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaaa aatt
//
LOCUS       DAZUMT010000070        14975 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000070 DAZUMT010000000
VERSION     DAZUMT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.070"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..632)
                     /locus_tag="ACG8FM_001958"
     CDS             complement(51..632)
                     /locus_tag="ACG8FM_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS5094609.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(638..1897)
                     /locus_tag="ACG8FM_001959"
     CDS             complement(638..1897)
                     /locus_tag="ACG8FM_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5094610.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1899..2945)
                     /locus_tag="ACG8FM_001960"
     CDS             complement(1899..2945)
                     /locus_tag="ACG8FM_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094611.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3180..3455)
                     /locus_tag="ACG8FM_001961"
     CDS             complement(3180..3455)
                     /locus_tag="ACG8FM_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS5094612.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACG8FM_001962"
     CDS             complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACG8FM_001962"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS5094613.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACG8FM_001963"
     CDS             complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACG8FM_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS5094614.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACG8FM_001964"
     CDS             complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACG8FM_001964"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS5094615.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7283..7918)
                     /locus_tag="ACG8FM_001965"
     CDS             complement(7283..7918)
                     /locus_tag="ACG8FM_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS5094616.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7984..9387)
                     /locus_tag="ACG8FM_001966"
     CDS             complement(7984..9387)
                     /locus_tag="ACG8FM_001966"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS5094617.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(9374..10405)
                     /locus_tag="ACG8FM_001967"
     CDS             complement(9374..10405)
                     /locus_tag="ACG8FM_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094618.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10436..10657
                     /locus_tag="ACG8FM_001968"
     CDS             10436..10657
                     /locus_tag="ACG8FM_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS5094619.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10822..11559)
                     /locus_tag="ACG8FM_001969"
     CDS             complement(10822..11559)
                     /locus_tag="ACG8FM_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS5094620.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11712..13052)
                     /locus_tag="ACG8FM_001970"
     CDS             complement(11712..13052)
                     /locus_tag="ACG8FM_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5094621.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13289..14812)
                     /locus_tag="ACG8FM_001971"
     CDS             complement(13289..14812)
                     /locus_tag="ACG8FM_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5094622.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         4244 a         3216 c         2495 g         5020 t
ORIGIN      
        1 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
       61 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
      121 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
      181 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
      241 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
      301 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
      361 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
      421 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
      481 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
      541 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
      601 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
      661 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
      721 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
      781 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
      841 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
      901 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
      961 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     1021 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     1081 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     1141 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     1201 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     1261 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     1321 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     1381 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     1441 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     1501 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     1561 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     1621 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     1681 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     1741 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     1801 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     1861 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     1921 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     1981 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     2041 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     2101 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     2161 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     2221 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     2281 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     2341 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     2401 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     2461 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     2521 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     2581 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     2641 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     2701 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     2761 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     2821 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     2881 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     2941 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     3001 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
     3061 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     3121 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     3181 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     3241 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     3301 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     3361 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     3421 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     3481 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     3541 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     3601 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     3661 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     3721 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     3781 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     3841 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     3901 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     3961 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     4021 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     4081 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     4141 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     4201 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     4261 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     4321 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     4381 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     4441 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     4501 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     4561 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     4621 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     4681 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     4741 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     4801 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     4861 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     4921 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     4981 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     5041 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     5101 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     5161 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     5221 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     5281 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     5341 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     5401 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     5461 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     5521 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     5581 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     5641 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     5701 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     5761 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     5821 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     5881 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     5941 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     6001 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     6061 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     6121 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
     6181 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     6241 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     6301 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     6361 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     6421 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     6481 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     6541 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
     6601 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
     6661 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
     6721 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
     6781 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
     6841 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
     6901 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
     6961 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
     7021 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
     7081 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
     7141 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
     7201 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
     7261 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
     7321 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
     7381 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
     7441 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
     7501 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
     7561 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
     7621 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
     7681 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
     7741 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
     7801 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
     7861 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
     7921 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
     7981 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg
     8041 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
     8101 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
     8161 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
     8221 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
     8281 catccatttt ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag
     8341 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
     8401 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
     8461 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
     8521 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
     8581 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
     8641 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
     8701 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
     8761 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
     8821 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
     8881 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
     8941 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
     9001 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
     9061 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
     9121 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
     9181 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
     9241 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
     9301 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
     9361 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
     9421 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
     9481 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
     9541 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
     9601 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
     9661 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
     9721 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
     9781 gttgcagata aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg
     9841 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
     9901 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
     9961 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    10021 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    10081 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    10141 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    10201 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    10261 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    10321 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    10381 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    10441 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    10501 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    10561 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    10621 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    10681 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    10741 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    10801 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    10861 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    10921 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    10981 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    11041 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    11101 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    11161 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    11221 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    11281 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    11341 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    11401 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    11461 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    11521 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    11581 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    11641 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    11701 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    11761 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    11821 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    11881 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    11941 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    12001 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    12061 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    12121 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    12181 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    12241 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    12301 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    12361 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    12421 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    12481 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    12541 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    12601 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    12661 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    12721 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    12781 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    12841 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    12901 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    12961 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    13021 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    13081 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    13141 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    13201 agctattgac ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    13261 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    13321 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    13381 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    13441 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    13501 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    13561 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    13621 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    13681 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    13741 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    13801 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    13861 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    13921 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    13981 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    14041 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    14101 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    14161 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    14221 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    14281 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    14341 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    14401 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    14461 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    14521 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    14581 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    14641 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    14701 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    14761 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    14821 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    14881 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    14941 cctattccct cctctttttt caggttttta aagct
//
LOCUS       DAZUMT010000071        14947 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000071 DAZUMT010000000
VERSION     DAZUMT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.071"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..967)
                     /locus_tag="ACG8FM_001972"
     CDS             complement(68..967)
                     /locus_tag="ACG8FM_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094623.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(1132..1956)
                     /locus_tag="ACG8FM_001973"
     CDS             complement(1132..1956)
                     /locus_tag="ACG8FM_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5094624.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(1967..3262)
                     /locus_tag="ACG8FM_001974"
     CDS             complement(1967..3262)
                     /locus_tag="ACG8FM_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS5094625.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(3278..3757)
                     /locus_tag="ACG8FM_001975"
     CDS             complement(3278..3757)
                     /locus_tag="ACG8FM_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS5094626.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(3763..4791)
                     /locus_tag="ACG8FM_001976"
     CDS             complement(3763..4791)
                     /locus_tag="ACG8FM_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS5094627.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(4808..5914)
                     /locus_tag="ACG8FM_001977"
     CDS             complement(4808..5914)
                     /locus_tag="ACG8FM_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5094628.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(6143..8740)
                     /gene="adhE"
                     /locus_tag="ACG8FM_001978"
                     /gene_synonym="adhC"
     CDS             complement(6143..8740)
                     /gene="adhE"
                     /locus_tag="ACG8FM_001978"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS5094629.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(9045..9425)
                     /gene="yajC"
                     /locus_tag="ACG8FM_001979"
     CDS             complement(9045..9425)
                     /gene="yajC"
                     /locus_tag="ACG8FM_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS5094630.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(9522..10667)
                     /gene="tgt"
                     /locus_tag="ACG8FM_001980"
     CDS             complement(9522..10667)
                     /gene="tgt"
                     /locus_tag="ACG8FM_001980"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS5094631.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            10885..11583
                     /locus_tag="ACG8FM_001981"
     CDS             10885..11583
                     /locus_tag="ACG8FM_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5094632.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(11751..14390)
                     /locus_tag="ACG8FM_001982"
     CDS             complement(11751..14390)
                     /locus_tag="ACG8FM_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5094633.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         4231 a         3294 c         2651 g         4771 t
ORIGIN      
        1 aataaccgca gtttctgctt ctatgtaaac aacagcagct actgcggtta ttttttagtg
       61 atcattctca ttgttcatct tcaaaatgtt cataatgaac caactgtttc tgtgtatcac
      121 gtttcaaaaa ttctttaggc gttatgccga actctctctt gaatgctttc agaaaatgcg
      181 gataagaaga aaaaccactt ttactatata cagcactaga gctgcggtat tctcgcaaca
      241 agtgcttggc atagatcagc tttttcttca ttacatactg atgaaacgta aatccagtgt
      301 actttttaaa ttctcgtgtg atatggtatt tgctgacaaa aaactgcttt tccatttcct
      361 ctaaagaaag cggatgagat aaattttttg agatatattg taacatttgt tccacacgct
      421 cattaggaac tacagcattc tgttcttgcg cactctcttc tttttgtaca acttgattga
      481 gaaaaatcaa gtattctacc agagcttgtt catattttat gtcttcaccg taaatttttt
      541 tatcaggagg atggtcgata aatgataatt tttgcttcaa tacttcctgt tctatttgca
      601 atacacgact cctcttctct cccattggtt gaaaacaagc cgatagatta gaccatggcg
      661 tggaacgact ttctagaaaa gaaggggtga caaagatata gacacgctcg aatacatctg
      721 aagattggcg gacgatacga tgcaaatcat tggaatggat gagcagcaca cttcctgctt
      781 tgatcgtaaa ttgtcgtccg ttcaaataaa aagtcgcttc tccctttaat gtagcatgga
      841 tctcatagaa atcatgatga tgatagatcg ttttattttg gctgacgaca tctttgacat
      901 gatagatttc atactgatca gaaaccatcg tattgacttg ttcaccgctg attttctttt
      961 ggagcatgtg ccgcacctcc ttttgttcac ttatgcacaa ttttattata ccacgttttt
     1021 tgtaatcaaa aagagctgct tttatatact cttataaaaa agaagagacg tggcatcttc
     1081 tagccccgcc ccctccattc taaatattag ttaaccaacg tcttctttac attattcaag
     1141 aagacttctt ccataacgaa tcccatcatc ttttgtttct tcaagaacca catacaggta
     1201 cggacgattc ttcatgatga tttctttgat ttttgtgtac tcgatcacac cgtctcccaa
     1261 atttaccggc acgatttttt cctgttcgac acgaaaatct ttcaaatgaa ccgcacatat
     1321 cttttcacca aaacgttcaa aagcttcttc taccaattgg acttgttctt gataatcact
     1381 acttgtgatc aaattggtcg gatctaaaat gattcctaga tagtccgaat ctactgcttg
     1441 gatcagctgc tctacccttg ctaaagaata caccggatga ttcaaacctg gttctatccc
     1501 caccatcatt tggtattttt ctccagcttt taccaagcga cggataacgg acacagcttc
     1561 agcaaatgct tcatctgtga aattttcttc cgtatattgg atttccggca aaacacatcc
     1621 agtttccgaa gcaaccatcg acgcaccaaa ggaagaagca tagcgaagat actgctcgaa
     1681 ctttgtcagc aactcttgtc gtatcgttaa atctggatga atcatattga tatagcaact
     1741 taaaattcct acggtcactt cttgcttttc taaaactcgt ttgacataat ttcccatccc
     1801 agaattgatc tcatttgcgc cagaaggtgt ttcaggaaac gaaagaccta aggcgagctg
     1861 cagtgtatga atcccttgtt cttttgtttt ttctgcaaga tcttctatcg tttggacttc
     1921 ggaaagatca tgtcctctaa ttgtaatgtt caacataact attctcctaa ctcagcatat
     1981 tgggtagcca catgacgatt tgcgggaagt aagtaatcaa aaacaagacg actaagataa
     2041 ccccataaaa aggaagaagc ggtttcagca ttgcctctgc ttttacgcct cctacacttg
     2101 caccgacata tagtcctgta cctactggcg gcgtgatgga gcctacacac agattcatga
     2161 ttgaaaacaa cccgtaatgt acaggatcaa cccctacact tgtgacgatt ggtaaaaata
     2221 ttggtgtaaa aatcaagata gcaggcgcca catccataaa catacccacc agtaataaca
     2281 caagattgac taataacaga ataataaatt tattatccgt tacgcccata atcaacgaac
     2341 taattgcctg aggaataccg gtaaacgcca tggcgaaaga catcatggaa gaagtagcca
     2401 ataagaacat gatcgtccct gacattgcta ctgattgaaa caacatatta ggtatatctt
     2461 ttagttttac cgttttatag taaaacatag caatcaacag ggagtaaatg acagctatcg
     2521 ctgaagcttc tactgctgta aatatccccg tcaagatacc accgataatg atgacaatca
     2581 atagaaggct aggaatcgcc tcttttatta tttttccagc atgagccatc tgtccttttt
     2641 cgacaacagg gtatttattc tttttagcaa cgatgaatgc tactagcatg attcctagcg
     2701 cccacaaagc gcctacgaga taacctccca taaacaaagc agagatcgaa gcaccaccag
     2761 caacaagtga atacatgata aaagcagtac ttggaggaat gaccatacct gtcggagctg
     2821 acgcgatatt gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact tgctgaggga
     2881 tcaacactcc gccaatcgcg gtagaagcgg caatggccga gctagagatg gaaccaaata
     2941 aagcattccc taacacattc gtatgagcca aagcccccgg cacgcgcccg acgaacaaca
     3001 tcgcaaaatc cactagtttc ttagctatcc ctccattatt catgataatg cccgagagaa
     3061 cgaaaaacgg aatagcgatc agcgaaaaac tattgatact tcccaccatt ttttgagctg
     3121 aactaaaagc agaaacatca aacggtataa ctaacaataa agtcatcatc gatgagaatg
     3181 caatacttat agcaatcggc atccctaata caagcaacac aacaaataca aaaaataaca
     3241 ctaatccggc ttctattgcc attttattcc tccattttta tattttttct tgaattgctg
     3301 gcttgttgct taatgtatcg attagattga tcaggctata gataatgatg agtacccccg
     3361 atacaggtac tgacagataa acatatccca taggaatact taaagaaggc gagatctgcc
     3421 ccattgtcaa tgtgacagca ttccatccgc catacaccat gatcatcgca gcaaatacca
     3481 gaaataaact ttgaacaaca acttctaaaa ttttactttt tttgccagtt aatttttcac
     3541 ttaaaaccgt tacggctaaa tgtgcttttt gtcccacaac atacgcggaa gacaacatgg
     3601 caaaccagac aagaccgaat cgaacaagct cttccgtgac cgtatttgga tttcctaaaa
     3661 ccgttctgga aaatacttgc cataaaacaa cagcgaccat cccaacaagc aaaacagctc
     3721 ccaggatctc taataccttg tttaaaatca ttttcatcac agctacgctc cttgctcttg
     3781 gatcttttga taaaggtctt tgtaggtagg atcgtttttg aatatatcat gaagtggggc
     3841 aactgcctct cgaaacggtg tattatctac atcattgaat tggactccat gttcttcttg
     3901 tgctaaagct ttttcttctt caattgcttt tgcaaaagct tcaatttcaa aaaccgttga
     3961 ttctttcgct gcttcttcaa tggctttttc ctgctcatca gttagctgtt ctttgatggc
     4021 atcattcatg atgatgatat ctggcacacg tgtatgctca tcataggaat agtatttagc
     4081 cacaccacca tgtccagctg tatacaagac aaattcattg ttttctgatc cgttgatcaa
     4141 attggattga agagacgtgt acacttcatc acttcccata ggtacgggcg aaccgcctaa
     4201 aagctctacc attttgatag cggtttcgct ttgcatcaca cggatcttct ttccctccaa
     4261 atcttccggc gtatggatcg gaccgtctac catataaaag ctgcgctgac cagaatcata
     4321 ataggtcaat ccggtaaatc ctaaattttt ggtcgattgg tagattggct ccattatttc
     4381 ttcattatcc atcaccttaa agaaatgttc ttcatcattg aatagataag gaactgaaaa
     4441 aatcgaataa tctttagaga aactttccaa cgctgaggca cttacttttg tgaaatctac
     4501 tgctcctgac tgtgtcaatt cgatcagttc acgttctcca cccagttgtc catctgggaa
     4561 atactggatc tgtacggaac catctgtttt ttcttttact aaacgcccaa attcattcat
     4621 tgcatcaatt actggctggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt
     4681 tacatctgca tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag
     4741 cagacctagg ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc
     4801 ctttctttta acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc
     4861 cataaggcat tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg
     4921 tcccgattga aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga
     4981 tagcacgata agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca
     5041 aaataccgta agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca
     5101 acaacgtatg ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt
     5161 caagtgtttc gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa
     5221 tgatctctgg aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat
     5281 agttatgatt tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca
     5341 agtatttgat atggatcata aactggtaac gagcttcttc taaatattct gggcgattca
     5401 acagtttccc gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact
     5461 gattcttatt ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca
     5521 tgtcattcat gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg
     5581 tgtcttcata caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg
     5641 gagcgccttc ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat
     5701 tttttgtcat ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc
     5761 acactgtcca gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat
     5821 tatcgtcttt gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt
     5881 ctttaggtac aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt
     5941 cattttatat ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa
     6001 aaactgtcta aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa
     6061 ttaaaaaaag aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc
     6121 ttttttctac gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt
     6181 cagagatcaa tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag
     6241 ccatacggtc tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca
     6301 tgttgatgcc gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt
     6361 cctctgttgt ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg
     6421 cacggaaata gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc
     6481 ggataatatg cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc
     6541 cgattttatg tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag
     6601 ccattgtaga agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt
     6661 tttctaaata gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg
     6721 aagccatgac tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg
     6781 ctgtgacaga tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg
     6841 tcaatgcata atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa
     6901 atggcgttac ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt
     6961 ctggtttgtc gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa
     7021 agaaagatgt gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag
     7081 aaccgcctcc aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg
     7141 cgtatacggt atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat
     7201 catttttacg tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac
     7261 cagggtcaca aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag
     7321 agttcttttc aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt
     7381 tcgctacagt tttgacattg attaagttta cagcagagac gttacgagaa acagagttct
     7441 taccataaga accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga
     7501 tgccgccttc tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga
     7561 atttgacatg caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa
     7621 gattcaacat accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg
     7681 caagaactgg agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt
     7741 cagcaatcag cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca
     7801 tcgcagaatg tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat
     7861 cttctaattt ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg
     7921 cttttactgc tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca
     7981 ttccattatc aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg
     8041 ttttttcaat ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag
     8101 agtaagcaga cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat
     8161 ggttcattag catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt
     8221 caggagctcc ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt
     8281 tttgggcact tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg
     8341 atttgaaaat cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga
     8401 ctggttcagc aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt
     8461 tatcatgttt gatattatgc caaatatatt cggacgcata catatttttg attgctttat
     8521 cttcgtaaac accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat
     8581 ccaaagctgc cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt
     8641 ccatttcttt caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt
     8701 caattgtctt cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc
     8761 atggttattt gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac
     8821 acttttttgt gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg
     8881 tttgtataga aaaatatata aagttattta ttattattta tatgacacac ccgattattc
     8941 aatattgagc aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta
     9001 ggacatcttt ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc
     9061 tcttctgtct cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt
     9121 tcttctgttg tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc
     9181 gttttgattg ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc
     9241 cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa
     9301 tcacctggtt tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa
     9361 cgtgacataa aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt
     9421 cccatttttt tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct
     9481 gataacacta gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt
     9541 tctttattga acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc
     9601 atgattgctt gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa
     9661 gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta
     9721 tagtttttac atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat
     9781 ttcgcatttt tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt
     9841 cgagtaggca agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa
     9901 tcagcagcac cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg
     9961 aattctaata cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag
    10021 taaccaggga aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac
    10081 cctgctcctt ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat
    10141 cctcgttctg cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca
    10201 tcgaatggtg gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg
    10261 ttgattgctt tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc
    10321 acgccttctt cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa
    10381 tctgttaaaa tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact
    10441 aagtcttcac cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc
    10501 atttctttca actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg
    10561 aacattggtg tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta
    10621 tgtttttctt tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact
    10681 ttctatataa aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa
    10741 gttccctcgg cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat
    10801 aacaaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt
    10861 attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa
    10921 gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt
    10981 ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac caccatatat
    11041 gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt
    11101 ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat
    11161 cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga
    11221 ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca
    11281 acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa
    11341 agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat
    11401 acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat
    11461 ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg
    11521 ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt
    11581 taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc
    11641 agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc
    11701 tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct
    11761 tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata
    11821 gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct
    11881 tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct
    11941 accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg
    12001 aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc
    12061 tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac
    12121 aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca
    12181 aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct
    12241 cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca
    12301 gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa
    12361 atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct
    12421 tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca
    12481 ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac
    12541 gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa
    12601 gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg
    12661 acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct
    12721 gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct
    12781 ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt
    12841 tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt
    12901 gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact
    12961 ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa
    13021 ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc
    13081 atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt
    13141 ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg
    13201 gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca
    13261 tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg
    13321 atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc
    13381 agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc
    13441 aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac
    13501 gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg
    13561 aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc
    13621 gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt
    13681 ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca
    13741 atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata
    13801 ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact
    13861 gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa
    13921 cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt
    13981 tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc
    14041 atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc
    14101 gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta
    14161 gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg
    14221 gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc
    14281 aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct
    14341 gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc
    14401 ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta
    14461 cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata
    14521 tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa
    14581 tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt
    14641 ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg
    14701 gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg
    14761 tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa
    14821 tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa
    14881 tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg
    14941 gcttttt
//
LOCUS       DAZUMT010000072        14679 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000072 DAZUMT010000000
VERSION     DAZUMT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.072"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..1033
                     /locus_tag="ACG8FM_001983"
     CDS             137..1033
                     /locus_tag="ACG8FM_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5094634.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1183..2124)
                     /locus_tag="ACG8FM_001984"
     CDS             complement(1183..2124)
                     /locus_tag="ACG8FM_001984"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5094635.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2250..3371
                     /locus_tag="ACG8FM_001985"
     CDS             2250..3371
                     /locus_tag="ACG8FM_001985"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5094636.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3629..5131
                     /gene="eat(A)"
                     /locus_tag="ACG8FM_001986"
     CDS             3629..5131
                     /gene="eat(A)"
                     /locus_tag="ACG8FM_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5094637.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5198..5725
                     /locus_tag="ACG8FM_001987"
     CDS             5198..5725
                     /locus_tag="ACG8FM_001987"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5094638.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5936..6997
                     /locus_tag="ACG8FM_001988"
     CDS             5936..6997
                     /locus_tag="ACG8FM_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5094639.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7000..7896
                     /gene="murQ"
                     /locus_tag="ACG8FM_001989"
     CDS             7000..7896
                     /gene="murQ"
                     /locus_tag="ACG8FM_001989"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5094640.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7910..9892
                     /locus_tag="ACG8FM_001990"
     CDS             7910..9892
                     /locus_tag="ACG8FM_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5094641.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9957..10403)
                     /gene="nrdI"
                     /locus_tag="ACG8FM_001991"
     CDS             complement(9957..10403)
                     /gene="nrdI"
                     /locus_tag="ACG8FM_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5094642.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10614..12038
                     /locus_tag="ACG8FM_001992"
     CDS             10614..12038
                     /locus_tag="ACG8FM_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5094643.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12219..12992)
                     /locus_tag="ACG8FM_001993"
     CDS             complement(12219..12992)
                     /locus_tag="ACG8FM_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5094644.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12989..13894)
                     /locus_tag="ACG8FM_001994"
     CDS             complement(12989..13894)
                     /locus_tag="ACG8FM_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094645.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14048..14368)
                     /locus_tag="ACG8FM_001995"
     CDS             complement(14048..14368)
                     /locus_tag="ACG8FM_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS5094646.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4693 a         2614 c         3103 g         4269 t
ORIGIN      
        1 agaagaatga tttatttcat catattttca tacaaatgtt aaattttcat acaaatatag
       61 gaaacgtttt actggctttt tactatttaa acaggctatc ataggaaaaa gtgacagaga
      121 aagtgaggcg gaaaagatga acaaaaatga cgtactgcta gttgcaaaaa ataaatcact
      181 ttattttttg ccttccatcc gctctttaga tgtttggtcc ggaaaagatt tactacagtc
      241 ggaattcatt aaatcagcta gacaacagtt ggcaaattat cagctgatcg tttttttaga
      301 ttatggattt gatatggaga tggctcgacg cgtgcgccca tttaccaatg caaaaattgt
      361 gttatttttt tggaatcact tcaaagcaga acacgaggcg atgctgaagc aggcacaaga
      421 tgaaccggca atcgatgaga tttatcattt tgattttttg gaagcaaaag aacttgggct
      481 gaaacataat agttcatttt attcgaaaca tatgaatttt tcttctagcg aaccaaagac
      541 ggatctattt tttggtgcaa cgaataatgg gagaaaagaa cgagcagaaa gctataagca
      601 ggaatttata aaaagagatg ttacgaccaa ctatttcatt cttccacatc gagggaatga
      661 acaacctgga tatcttactt atgaagagta cctgaaaaaa acaggggaat cgcgcgggat
      721 tctggaactg ttgcgggagg gtcagcaagg agtgactttg cggacatttg aatccatgta
      781 ttttcaaaag aaactagtga cggataatca agcgattgct cattatcatt tttatcgtcc
      841 tgaaaatatt tttttgcttc gagaacgtga tttagatgaa ttaccggaat ttctgcatac
      901 accatttaag ccaatcctat cagagctatt ggaattttat gatgcagaaa attgggcaaa
      961 acgatttatc actaacgatt ccactattta tgagcaatac gaatatgacc tctcctctgt
     1021 tgaaaacaaa taaaaaacct acattttgaa gagattatgt actgaaacaa acaagcacaa
     1081 ttcttatggc atcatgagag ttgtgttttc tttttgatct tttattgggc ttaaaaaaag
     1141 agaaccagct gctgattcag ctgattctca tcagagagcc ttttattctg cgccaaggat
     1201 tccttctttt gcatggacta attgggtcaa aaaggcacaa tatggtgtag caatatcata
     1261 tttttgtcct tttcgccaaa tggcaccatt gatgtagtcg atttcagtca aacgatggtt
     1321 tttgatcagg tcttggtaca tagatgggta atgcaacccg atgttgtcag gatcataagt
     1381 agaagcaata tgttggtaca cttcttcttg atctagtatg attccttctt ttgctgcgat
     1441 agaagcaaat tcagatacga ttgttcgaac catgctctct gccgcttttt gcgcgcctag
     1501 ttcagcaata ttacaatcca aaatcgtgca taaaccattc aatgttccat ttacacaagc
     1561 ttttcgccaa atggaataac gaacgttatg gctatagatc ggatttaaat ttgcttcttg
     1621 aaagacttct acaacttttt ttgtaaatgc ttcgccttct ggttccaagt tctctagttc
     1681 gatttctcca tcacctaaca atgtgacttt tccgggacca gctagaccag ctgtccacat
     1741 cgtgatccca aataagatat ttttctttgg cacatatttt tccagtacat cttcatgacc
     1801 taatccattt agtaaacaca aaacataagt attgtcagta ataatggatt ggatcgaaca
     1861 aaacatatca tctaattgat tggcttttgt taatgctacg atcaagtcca cgtgttcgtt
     1921 gctttcgatg atttcttctg gtgaatagat tggcagtttt gcgaccactt ctttaccgtt
     1981 aaagtcagca atcaacccat ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat
     2041 caagaggacc tcatttccgc tttgatgtag catcaatccg attcggctgc ccattgctcc
     2101 ggccccagct attgcgattt tcatgttttt tctctccttt attcaatgag ttccctttta
     2161 gtatatagaa aaatggaaga aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt
     2221 cttacttaag aagaaagtga gggatacaga tggatatttt ggcggcaatt gattcattta
     2281 aaggaagtgc ttcttcattt gaactaaatc aggcagttct aaatggctgg gaagatcgta
     2341 aaggaagaaa aatcaatgtg ccgattgcag atggcggaga aaatactgca gaagcaatct
     2401 attacgcgct caggggaaca tggcgtacca ttgatgggat ggatctctta ttccgtcagc
     2461 gaaaaatcag ctatttattg acaacttggc aaggaaaaaa caccgcagtc atcgaatctg
     2521 cacaagcatt ggggttagat ctacttgata aaccaacgga tcaaactatc cgacttgctt
     2581 ccagttatgc cttaggagaa cttattaaag atgcactgct tcaagaagta gaacaaatta
     2641 ttgtcacttt aggaggaagc gggtgtagcg atggtggtct aggattactc caatccttgg
     2701 gtgcttcttt agaagggtac acagaaggaa atcctttatt atctactaaa aaacttcatt
     2761 ttgatcgaag aaacgttctt gtttctaaag taaaattgat tatcgctgca gatgttacca
     2821 atccatatac aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag
     2881 atacgacgct agctttctta gataaacaag caggaaagat tgctcagcaa ataaaagatt
     2941 tctgggaggt ggatttagat gatattccag gaagtggagc agcaggcggt attggtggag
     3001 cccttttttt gttgggagcg aagatggttt ctggtttcga tttagtcagc caaatcactg
     3061 gattggaaaa aaagatcgaa caagcagatg tcatcattac aggagaaggg cggatcgatc
     3121 agcaaacgat ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa
     3181 aaccaataat cgctatttgc ggaagtagag accaagagct agaaggactt tctgattttc
     3241 tcccaattgt cttaagtatt catttgggtc caataggttt agaagaagca atggaacaca
     3301 ggcaaacatt agaaaaaaca aaaatcttag gacagacact tagcagattt ttcaccatcc
     3361 tcccgaagta gttgacaaaa tcattttctt tatgctatcg taatttcaaa tataggacaa
     3421 agtgagggat ccgtgcattg aaaaattaag tcgattgatg aaagctgcat tcagactgta
     3481 ccttgtggag acaaggaagc agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt
     3541 atgggtcttt ttgtgtgtat ccaacccttg catcctcttt agccagttct gaaaatagga
     3601 ctggtctttt tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa atctgacatt
     3661 cggctacgac agccaaggca cattattatt tgaacaagca aatctaaatt ttgacacaca
     3721 atggaaacta ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct
     3781 acaaaacaaa ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc
     3841 gcaacagaca aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt
     3901 tgagatatgg gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg
     3961 gagagaattc agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt
     4021 tgtggatgac actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg
     4081 tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca
     4141 tgaccgggga tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact
     4201 ggaactttat caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt
     4261 tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga aaacagcagc
     4321 tgaaaaagcc gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt
     4381 catcgatact gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac
     4441 gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa
     4501 actattaaaa gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca
     4561 taagcgactt ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc
     4621 aattcatttt acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg
     4681 aaagtcatcc attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa
     4741 ataccagcca aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg
     4801 aacgttggcg gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct
     4861 ccgaaagctt gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg
     4921 ccaacggaaa aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg
     4981 ggatgaacca ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt
     5041 gaacgtgaaa cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc
     5101 tactgagatt atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat
     5161 ggttaagggg aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg
     5221 agaaacaact tggaacgcac aaggattagt ttgcggacgg gcagatgtgc cgctgactga
     5281 aaaaggacag atgcaggcac agaaactagc agaaaaggta gtagatttgc ctgttccaat
     5341 cacgaaaatc atccattccc ctcttcaacg agcaagagat acagcacaag cagttgctga
     5401 tcgtctcagc ttgccgctga ccgttgatga acgattggta gaaatggatt ttggggatta
     5461 tgatggactg ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg
     5521 attcccaaat ggggagtcag tcttagatgt gtatgcacgg atcgttcctt tattgaaaga
     5581 atgcatcgaa gacgaagaaa atgtctatct gttagtttgc cataatgcct tgataagagt
     5641 gatcaatgct tattttcatc cgatgcctaa tgaggggttc tttacattca tggtagataa
     5701 tacagaattg gtttcttatg agtaattatg tttttgtgtt atttaaataa aatatttcaa
     5761 ggcactgctt ttgcttagca gtgccttttt taggttatga caattctttt gataatataa
     5821 actcaacctt tagaaatgaa atttaattat tttaataaaa gtattgaaat tttgtttctt
     5881 tagatatata ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt
     5941 tggcttttca atatttatga acgattcttt gtcagaagag aagaaacagt atatccgcca
     6001 aatggcatcc aatggctttg tcggtatttt cacttccatg catataccag aagatgatgt
     6061 ttcggcatat caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga
     6121 attgatggtc gatatttcag gagaggcatt ggaacgagcg ggtttttcat tagaagatct
     6181 aacgcctttg aaagcaattg gtgtaacagg tttaagaatg gattatcata tttccaatca
     6241 gcagattgcc gactggtcac atcaattgaa aatcagtttg aatgctagca cgatcacgcc
     6301 taaagatatc gatgaattga aagaagcaga agctgatttc tcacagattg aagcttggca
     6361 taattattat ccacgtcccg aaacagggct ggacaaagaa tggtatcaga aaaaaaatca
     6421 atggctcaaa attcaaggtc tccttgtcca aggttttgta ccaggagata cagagttgag
     6481 aggcccacta tatcaaggat tgccaacttt ggaagaacat cgagggatcc atcctcttgc
     6541 aggcgctcta gatctattag caagtaacac ggatattgtt tacatcggag atgcaggtct
     6601 gtcagaaaac gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca
     6661 tacggaacct gtagatgaag aattttacga atatatatta gggaagcata cgaatcggca
     6721 agacgatgca agagatgtca tacgaagcgt agatgcacgt tttcgagaga ttccgcctat
     6781 cccagcaaga aatacagcta cgcgtatgaa agggtctatt actttagata acgaaaagta
     6841 tttgcgctat atgggtgaga tccaattaac caaatatgat ttgccagcgg atgaaaaagt
     6901 gaatgtagta gctaaagtta tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg
     6961 tatgaactat caatttataa gaaaagaagg aagataaaaa tgagcaaaat caatttagag
     7021 aacctaacaa cggaacgcag aaaccaagaa acttttggat tagatgagat gagtatttct
     7081 caagccgttc aactaatgaa caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa
     7141 ctgacaagta tcgaaccagt gattgctaac acgattgagt cctttaaaaa aggtggacgt
     7201 ctgatttata tgggagccgg aacaagtgga agactaggtg tgttagatgc tgctgaatgc
     7261 gtaccgacat tcggtgtaga accggaaatg gtcataggtc taattgctgg tggagaagaa
     7321 gcaatgactg tagctgtcga aggggccgaa gattcttatg agctagggca agaagatcta
     7381 atcaagctga atttgacaaa aaatgatata gttattggta tcgcagctag cggtcgtaca
     7441 ccatatgtga tcggtggatt agattacgca aaatcgatcg gtgctgtcac aggaacgatt
     7501 tcttgcaata aacaagccga aatcagtaaa cacgcggatt tcccaatcga agtcgatgca
     7561 ggtccagaat ttttgacagg ctctactcga ttgaagtcag gaacagcaca aaaacttatc
     7621 ttgaatatga tttctactat cagtatgatt ggtataggta aagtattcaa taacttgatg
     7681 gtcgatgtca aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat catcatgcaa
     7741 gcgacagaag ctgattacga aacagctgaa aagtactttg aagaagcaga tcaaaatgtt
     7801 aagctggcta tcgtcatgat tttgacaaat agtgataaag aggaagctca agcaaaatta
     7861 gctgaagcaa acggatttgt aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa
     7921 cgaactgact ttggaagaac tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa
     7981 tgtagaaagc attgtccact gcatgactcg tgtgcgtatg aagatcatca atgatgatct
     8041 tgtaaatgtc tctgaattaa aagcgattcc aggagtgctt ggtgtagtag aagatgaaca
     8101 gcttcaagtc atcatcggac ctgggaaagt gaacaaagta gcacagcaaa tggttgattt
     8161 ggcaggcgtg aaattaggag aaaaactttc ttctgtaagt acagctacag caaattcatc
     8221 agcatctgga aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc
     8281 gaaacaaaaa ccatctaaaa tcaaatcggt attaaaagac atttctaata tctttgtgcc
     8341 aatgattcct gcatttgtcg gtactgggat cgtggcaggg attgccgcag ttttagcaaa
     8401 cttagtcaca gccggaacac ttgatgcagc aacatggcaa caatatatcg atgtgatgaa
     8461 catcttgaaa aacgggatgt ttagctacct agttatctat acaggtatca atgctgcaca
     8521 agtttttggt gcaacaccaa ctcttggtgg agttatcggg gccgttgtga tgctgacagg
     8581 gatgaatccg gaagcaccac tgaaaaactt atttactggt gaagctcttg cagctggaca
     8641 aggtgggatc ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg agaaaaaact
     8701 gcataaagta gtacctgatg cggtagatat catcgttaca ccaactcttt cattgattgc
     8761 catcggtctg attgaaattt tcttcatcat gcctttagca ggtttcattt ctgatggcat
     8821 ggtcggtggg atcaactggg tacttggcgt tggtggagca tttgccggct tcgttctagg
     8881 cacattattc ttgccgatgg ttatgttcgg actgcatcaa attttgactc caatccatgt
     8941 gcaaatgatt gatgaaactg gtcgtacgct attgcttcca attcttgcga tggctggtgc
     9001 aggacaagtg ggtgcagcat tggcattatg gattcgctgc aaaaaagata aagaattaac
     9061 agaaatgatc aaaggagctt taccagtcgg gatcctagga atcggcgaac cattgattta
     9121 cggagtaaca ttacctctag gacgcccatt tattactgct tgtatcggtg gaggtatcgg
     9181 cggggcagtg atcggaatga tcggaaatgt cggggctatc gctattggac cttcaggagc
     9241 cgctttgatc ccattgatct ctgacggtaa atggttaggc tacatccttg gattattggc
     9301 agcttatgct ggtggcttcg ttgctacatt cttcttcggt attcctaaag accgtttaga
     9361 aaaagaagaa ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc
     9421 tcaaacttca gctgaaagtg tgactatcct ctcatcagtt gctgacggaa cagttgtttc
     9481 tttagataaa gcaagtgatc cagtttttgc acaaaagatg atgggtgaag gctactttgt
     9541 agaaccagaa aacggacata tctattctcc agttactggg gtagtcagct ctgtcttccc
     9601 aacgaaacac gctattggga tcacaacgcc aaatggttta gaaatcttgc ttcatatggg
     9661 aatcaacaca gttgacttag gcggtacgcc atttgatgtg aaagtaacag aagggcagca
     9721 agtgacacct gatacgcttg tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa
     9781 agaaacatcc atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga
     9841 aaaaacaggt aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt aatatgatag
     9901 aaacaataga aacgaaagaa gaaactgaaa tctatttttc agcttcttct tttttattat
     9961 ttattgtttt ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt tccccgtaat
    10021 tcataatctg ccaagaaagg aaagccaaat gtttgtgcat attgtttggc tgttagacaa
    10081 tactgattat tgaagttttt atttccgcta ccgactacac caagacaaag tttatgattg
    10141 tcttcatgtt ccagatattc acgcatcgtt tcggtcaaga tttctgtatc accgttatct
    10201 aaaccatttc cgccgtctaa ataagtagga acgaaagtaa aaaatggttc tgtttctttc
    10261 gtaaaatcac tattttcatg gatttctttt aaagtaactt caggattgac aggatcttct
    10321 gcatgcattt tttcagcata ttctgctaga tgtttggcaa aggcacgcgt atttcctgaa
    10381 atcgatatat ataaaatgtt catggatcat cctccaattc tttcctttta gtatagcatc
    10441 ttttttaagt tttttggtgg tgtaatttgg aaagggacta ggcgctttgc ttgtataatg
    10501 agaaaaaagc cggtagaatt atatttctat taaagttttt attaattaag taaaaataaa
    10561 aaaggtttgt tatacttttt gataaaagcg gttttatttt ggaggatgct tacatggata
    10621 cagagcaaca gaaaaaagga attggtttaa ttccacttgc tgccttagta attggttcag
    10681 cgattggagg tggcgtattt ggtattatga cggacatctc cggatcggct ggcggcccag
    10741 ctattttcag ttgggtactc gtaggaacag caatgttgat gctctccttg tcgatcaata
    10801 atatcaataa aaaacgtcca gatttaaaag gcgggatctt tagttacgca gaagaaggtt
    10861 ttggccgttt cgctggtttt atcagcggtt ggggttactg gctcacatgc tggttaggca
    10921 acgtagcttt tgcaacattg ttgatgagcg cattaggata tttcttccca gtatttgaag
    10981 gtgggcaaaa tctggcatcg atcgtgatga gtacagtctt tttatgggga tatgcatggc
    11041 tagtgaatcg gggagtcgaa aatgctagtt tcgtcaatgc tatcgtcaca ttctgtaaat
    11101 tagtgccatt atttttattt attgtagtag ctatcagcac gttttcgatc caacaattca
    11161 ttgataactt ttctagcgac attatattga atgcgaatgg acagccgatc ccgttttcca
    11221 gtcaagtatt gagttgcatc atggtgatgc tttgggtatt tgttggtatt gaaggagcct
    11281 ctgtcgtagg ttcacgagca aaaaaacaat cagatgttgg gaaagcaacg attctaggga
    11341 tattcggctt gattattatt tacgtcttgg tttccatgct tccatatggg acaatgacga
    11401 tgaatcagct gcaaacgatc aaggcgcagc ctgctatggg ctatgtgttt gaaatgatgg
    11461 ttggtaaatg gggagcagtc gtgatcaacg gcggtttgat catttcttta gttggtgttt
    11521 ggctctcatg gacgatctta ccgattgaaa caatgggaaa catggctgaa gacggacttt
    11581 tgcctaaagc atggggaaaa ctcaacaaaa atggggcacc aacttatgct atcgtattga
    11641 cgacgttatg tacgaatgtc tttttacttt cattattatt cactgacagt gcttataact
    11701 ttgcttacac acttggtaca gcagctattt tcttcacgtg gcttttctta ggattgtacc
    11761 aaatgaaact ttcttatcag cggaaagaat gggtccaatt tttagtaggg cttttagcaa
    11821 gtgcctttca agtatgggct atgctagtcg tagcctttaa tgaagtaatg cttgttcttg
    11881 ttcttttctt gccaggcatt tatttttacc tcaaagcacg taaggagcaa aacgagcctg
    11941 tacaacatat ccacttagat aagattttac ttataggtgt gacgatggtc ggagtgattg
    12001 caatggtttt atttgccact gggatgcttc acgtatagaa gtataaaaga agtataaaga
    12061 gaggttgtga gcctgtcgtt tattcaaaaa catactagga aaggagctgt gacacaaatg
    12121 ttgtcacagc tcctttttca gaataaacgg tgtttaagag acagccaatc ggcaataagg
    12181 ctgaacgtct ctttcacagc ctcttttttg atgagtcatc atacattttt tcgcttaaat
    12241 acgagatatc ctaatgctaa gaacaaaacg atgtaagcta aattaccgaa tagcatttga
    12301 ttagtagaaa gatgagtcaa ttgcttcatg atctcttcgt ttccaatttg gttttgcaga
    12361 ttcaacatac tgatcggatt ccatttgatc cactcccatt tttggatgag tgcaataagg
    12421 ataccagata tgattgacga agcaaaataa aagacgattc ctgctgagat ggatgcgccg
    12481 gaactattga taaagcaagc aatcatcaac actaaactta aaatcagcca taaaccgata
    12541 taactaccca aagtataagt gaacaagctt tggatcagtg tttgaccagt agaaaccttc
    12601 tccgtgaagg aaatcgaagg gaaaagaatc agtttcatca acacagtgac aataatagct
    12661 aaaaggtata aataaacaga ataaataact aatgtgatcc atttgcttac taatatttct
    12721 cctcgagaat actttcgata aaggagattt ttcaatgtac catattgtgc ttccatcgaa
    12781 atgatcgtgc ttgcagctgc aatcatgata aagacaatcc aagatgtggc actaaaaagc
    12841 tgagcagtca tttcaactgg atcaatgatc gtcgtgtatt ttttggctag aagtgctgtt
    12901 cctatgagca aaacaactaa tagaacagat aaaacagcag ttgacttttt cttgatcaat
    12961 ttaaataatt cttgttttac aagcaatgtc atttttcttc ttcccccttt actgaatcca
    13021 gaatttccaa tagagaagat tccaaatcat tcgtcagatg ctggacgtct gtcacatgga
    13081 tattttgtgc agacaatagc tggatcacgg ttcctaataa ttcttcgttt tcttttaaaa
    13141 gcttgatttt atcaggatct tccaaagtat atccagcttc tgtcaagatc gttcgagcag
    13201 ctagatcatt tgttgtagag ataacgatga aatcttgcgc tgtttggttc aattcagcca
    13261 ttgtacattg tttgatgatc tttccttgat tgatcaggat cagctcatcg accatttttt
    13321 caagctcgct gagtaagtga ctggagatca agaaagaggt gccctgagta gccaagtcgg
    13381 taatgatttt tcgcacatct cttgttgctt gtggatccaa accgttcatc ggctcatcta
    13441 aaataacgat agacggatgg ttcagtaagg caagagcaat tcccaatttt tgtttcattc
    13501 ccaacgagta ggatttcgct tttttgtgaa tgtacttttc catcttgagc tgattcacga
    13561 tagtgtcgat agcagataca tcgttggttt cagagaataa cttcagatga tcatagcctg
    13621 ttaagaatgg atagataccc gggtattcga tcaatgctcc tacttgttcc aaccctttat
    13681 gactagaggg agaaatctca ttttcaccaa tacagattgt tccatctgag taatggatca
    13741 gtcctaaaat agctttcatg atcgttgttt ttccagcgcc attaggacct actaatccaa
    13801 cgattttgcc aggggataag ctaaaactga catcgctgag ggcttgtttg tgaccaaagt
    13861 tcttggataa ttcttggacc gaaagacttt tcaaaaatat tcactccttt tctatagata
    13921 gtacaagcat acacctgatg agtggagaag tcgctaggtc attggactta tttcttattt
    13981 tttcataaaa aaattgaata ttaaataaaa aggcttattt tttctgatca gtacttattc
    14041 caagtgtcta gtggccgcta gtaatcttga tgccaatgat actgatgagt agcatcccca
    14101 caaagaacca agtggaaaga ttcagcttat catgaaacaa gaccacacca attatgaccg
    14161 aaccaactgc gccaatccct gtccagatag gataagagag actcatcggc aaacttttga
    14221 ttgcctttga caaaaagtaa aaactagcaa tcatgccaat cactgtgtaa aaagaataat
    14281 tgattttaga aaaaccatca ctccatttca tcatcgttga ccagaatact tccaaaattc
    14341 ctgctatgat taattccatc catgccatac ttacattcct ccttaactat aaaaaaatac
    14401 acaaaaagag gaaaatcatc ccttcaaaga cctaatggaa tgatcatcct ctttatccta
    14461 cccggtggca gattgtatat ctttttctgt agtatagcac acctatccag atatcggcaa
    14521 tctcatactt tttagtaaag tgtaccaagc actgacggat atatcgttag cggaaaagaa
    14581 gatgaatgct ttgcaatttc atagaaagaa gttgttattt attaatcatt taaaaaaaac
    14641 agtaactaga ccttatttct atatattatc gggcaataa
//
LOCUS       DAZUMT010000073        14189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000073 DAZUMT010000000
VERSION     DAZUMT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.073"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..483
                     /locus_tag="ACG8FM_001996"
     CDS             <1..483
                     /locus_tag="ACG8FM_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS5094647.1"
                     /translation="FGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIET
                     ALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNG
                     ILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI
                     "
     gene            805..975
                     /locus_tag="ACG8FM_001997"
     CDS             805..975
                     /locus_tag="ACG8FM_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094648.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            989..1591
                     /locus_tag="ACG8FM_001998"
     CDS             989..1591
                     /locus_tag="ACG8FM_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5094649.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1607..2284
                     /locus_tag="ACG8FM_001999"
                     /pseudo
     CDS             1607..2284
                     /locus_tag="ACG8FM_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2595..3092
                     /locus_tag="ACG8FM_002000"
     CDS             2595..3092
                     /locus_tag="ACG8FM_002000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFS5094650.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            3093..3509
                     /locus_tag="ACG8FM_002001"
     CDS             3093..3509
                     /locus_tag="ACG8FM_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFS5094651.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3529..5073
                     /locus_tag="ACG8FM_002002"
     CDS             3529..5073
                     /locus_tag="ACG8FM_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5094652.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5216..5440
                     /locus_tag="ACG8FM_002003"
     CDS             5216..5440
                     /locus_tag="ACG8FM_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094653.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5455..6324
                     /locus_tag="ACG8FM_002004"
     CDS             5455..6324
                     /locus_tag="ACG8FM_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5094654.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6305..7039
                     /locus_tag="ACG8FM_002005"
     CDS             6305..7039
                     /locus_tag="ACG8FM_002005"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5094655.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7072..7980
                     /locus_tag="ACG8FM_002006"
     CDS             7072..7980
                     /locus_tag="ACG8FM_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS5094656.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7977..8519
                     /gene="sat4"
                     /locus_tag="ACG8FM_002007"
     CDS             7977..8519
                     /gene="sat4"
                     /locus_tag="ACG8FM_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS5094657.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8612..9406
                     /locus_tag="ACG8FM_002008"
                     /note="aph(3')-IIIa"
     CDS             8612..9406
                     /locus_tag="ACG8FM_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS5094658.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9682..9813
                     /locus_tag="ACG8FM_002009"
                     /pseudo
     CDS             9682..9813
                     /locus_tag="ACG8FM_002009"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9889..10382
                     /locus_tag="ACG8FM_002010"
                     /pseudo
     CDS             9889..10382
                     /locus_tag="ACG8FM_002010"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10552..11448
                     /locus_tag="ACG8FM_002011"
     CDS             10552..11448
                     /locus_tag="ACG8FM_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5094659.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11547..11756
                     /locus_tag="ACG8FM_002012"
     CDS             11547..11756
                     /locus_tag="ACG8FM_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS5094660.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11773..12045
                     /locus_tag="ACG8FM_002013"
     CDS             11773..12045
                     /locus_tag="ACG8FM_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5094661.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12047..12910
                     /locus_tag="ACG8FM_002014"
     CDS             12047..12910
                     /locus_tag="ACG8FM_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5094662.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13173..13910)
                     /gene="erm(B)"
                     /locus_tag="ACG8FM_002015"
     CDS             complement(13173..13910)
                     /gene="erm(B)"
                     /locus_tag="ACG8FM_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS5094663.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14035..14118)
                     /locus_tag="ACG8FM_002016"
     CDS             complement(14035..14118)
                     /locus_tag="ACG8FM_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS5094664.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5018 a         2222 c         2928 g         4021 t
ORIGIN      
        1 tttggtcacc ttgaaggtga tacgatcgtt ggcattcatc ataaaagtgc cgtcattact
       61 ttagttgaaa gattatctaa agtcattatc acgattaaac ccaacggccg taaggcatta
      121 gatattgaaa ctgcccttaa tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct
      181 attacgtttg actgtggaaa agaattttct aactggaaag ccattagtaa ccaacatgat
      241 attgatatat attttgcgga ccctggaaca ccttctcaac gcccattaaa cgagaattct
      301 aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg attttagaaa agtgaatcag
      361 acatttattt ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac
      421 agaacaccaa tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt
      481 tgacaaatca tactatgata cgtgtttgtt tttctgtgct gtttagtgaa tgattagcag
      541 aaatatagag agtaagattt taattaatta ttagggggag aaggagagag tagcccgaaa
      601 acttttagtt ggcttggact gaacgaagtg agggaaaggc tactaaaacg tcgaggggca
      661 gtgagagcga agcgaacact tgatctttta agttgctatc tttttaaggt caatagagta
      721 tacttatttg tcctaatatg gttttagcag tataattgac ttggtgaata ggtcatttaa
      781 gttgggcata ataggaggag taaaatgaaa aaatttattt atcgagtttt agaaaatgac
      841 gaagtggtgg ctatttttaa tgagcaacaa tatgcgcaag attttatcgc ttacgaaaag
      901 acaatttctg ataagcaatt tgaaattgaa aaagtagata tagctgattg gttattgcaa
      961 ccgagagaat tttagaggtt ggttgaaaat ggctaaaatt ggttatgcac gtgtcagtag
     1021 caaagaacag aacttagata gacaactgaa agcgttagag ggcgtttcta aggtcttttc
     1081 agacaaatta agcggtcaat cggtcgaacg cccacaatta caagctatgc ttaactatat
     1141 tcgtgaaggg gatatagttg ttgttactga attagatcga ttaggacgaa ataataaaga
     1201 attaacagaa ttgatgaatc aaattcaaat taagggggca accctggaag tcttaaattt
     1261 accctcaatg aatggtattg aagatgaaaa tttaagacgg ctgattaata atttagtgat
     1321 tgaattgtat aagtaccaag cggaatctga acgcaaacga attaaagaac gccaagccca
     1381 aggaattgaa attgctaaga aaaaaggaaa attcaaaggg cgacaaccta aattcaaaga
     1441 aaatgatcca cgtttacaac acgctttcga tttatttttg aatggttgtt ccgataaaga
     1501 agttgaagaa caaactggaa ttaatcgccg aacgtttaga aggtatcgat caagatacaa
     1561 cgtgacagta gatcaaagaa aaaaacaatg aaaagaggga tagttaatga gtacggttat
     1621 tttagctgaa aaaccaagcc aggcattagc ctacgcaagt gctttaaaac aaagcaccaa
     1681 aaaagacggt tattttgaga tcaaagaccc actatttaca gatgaaacgt ttatcacctt
     1741 tggttttggg catttagtcg aattagcaga accaggtcat tataacgaaa agtggcagaa
     1801 ctggaaactt gaatctttgc cgatttttcc tgatcgatac gattttgaag ttgcaaaaga
     1861 taagggaaag cagtttaaaa ttgttgctga acttctcaaa aaggcaaata caattattgt
     1921 cgcaacagat agcgacagag aaggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa
     1981 tgccttttct aaagataaaa cgtataaaag attatggatc aatagcttag aaaaagatgt
     2041 gatccgaagc ggttttcaaa atttgcaacc aggaatgaat tactatccct tttatcaaga
     2101 agcgcaaaca cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttatatac
     2161 cttgaattta caacaaaaag gcgtacaagg tacattttca ttaggacgtg tacaaacacc
     2221 gactttatat ctcatttatc aacgccagga agcaatagaa aactttaaaa aagaaccttt
     2281 tttcctgaat aattcatagt tttactaata gtctttccta acattgattt aatagtcttt
     2341 aaagtattct gcttcatcac ccattaggtg atgaaaaaag aacccctttc tgatttagcg
     2401 gacaaatact ttgacctaat ctcagaagat ataaaagaaa aaataataaa atatccgatt
     2461 tatactttgt ataaagaact aaagtagtta gcaaatcatt tacagcaaat cactatgttc
     2521 cacataccat tttacatact taccttatcc acgacacatg ttgagatagt atcgaagata
     2581 aagaaagttg ctccatgttg tttttgcgag atggggaaat caaagccaca ttgacaacca
     2641 tgatgaataa gcttgctttc agtcataagc taatcctaga accacttttc aagtcagtta
     2701 gccaaatcga tgaagaaagc gaccgtgaaa gaatggacgc tattgataag ctaatggagc
     2761 aactattgga agaacgcaat acccttattg cactgatgtc caaaggtttc ctagaacctg
     2821 ctctttttaa tcaggaacga aatgttttag atagtgagat taaaaatctt actaccgaaa
     2881 aaacaaacct ggtaacgaac tctgcgagtg gggttttgcg agcaaacgag ataaaggacc
     2941 tcattaatta tgtgtcagca gataatttta atggtgacta cacggaagaa ctatttgaag
     3001 aatttgtagt gaacatcatt gtaaattcca gggatgagct gacattcaat ttgaaatgcg
     3061 gtctttccct gaaagaaaag gtggtgagat aaatggcata tattccatat ggatacaaaa
     3121 ttcaagatgg agtggttact gtcgatgaaa aggcagcagg tcaagtaaag gtattctttg
     3181 agaaatacat atcaggacta tcccttacag tggctggcga acaggcaggt attgaaaaga
     3241 cacattctgt gatggggcgc attttgaaaa acgtcaacta ccttggaaat gatacgtatc
     3301 cagcaattat tgataaagag atatttgata aagctgaaga agttagagat aaacgtgcaa
     3361 aggatttagg acgagtggta gagcttgccg ctttcacctc tccccctccc aaagaacgat
     3421 tcaaaatgaa aaaggcagat aataagatgc cagtggatcc ttttgaacga gcagaatact
     3481 tatacagtct gatagaaagc gaggaataaa gtgacagaga aaaatataat ggttattcct
     3541 gctcgtaaaa gagtaggaag tacagccgca aaagaaaaga taaagaaact tcgtgttgct
     3601 gcctattgcc gtgtttctac agaaacagaa gaacaaaatt ctagctatga ggttcaggtc
     3661 tcacactata cagagtttat aaagaaaaat aatgaatggg agtttgctgg catctttgca
     3721 gatgatggta tctctggtac gaacactaaa aagcgtgacg aatttaatcg catgattgca
     3781 gagtgtatgg acggtaacat cgacatggtt attacaaaat ccatcagccg atttgcgcgt
     3841 aacaccctag actgccttca atacattaga cagctcaagg ataagaacat atcagtttat
     3901 tttgagaaag agaacatcaa caccacggat gccaaaggtg aggttttgct gactattatg
     3961 gcatctttgg cacagcagga aagccagagc ctttcacaaa acgttaagct tgggctacag
     4021 taccgatacc aacaaggaaa ggtgcaggtc aaccataagc gatttatggg ctacacgaaa
     4081 gatgaagatg gaaatttaac tattgtcccc gaagaagctg agattatcaa acgcatctac
     4141 cgagaatacc ttgagggtca gagtctagtg ggcattggtc gagcccttga aaaggatggt
     4201 attttaacag cagcgggaaa accaagatgg cgaccagaat cagttaagaa gatacttcaa
     4261 aacgaaaaat acatcggaga tgcccttctg caaaagactg tcacagtaga ttttctgacc
     4321 aagaaacgag tgaaaaatga agggcatgtt ccccaatatt atgttgaaaa tagccatgaa
     4381 gcgattattc ctaaagagtt atttttgcag gcgcaagaag aaattcatcg gagaagcaat
     4441 atctacacag gagaaggcaa gaacaaaaga atttacagta gcaagtacgc tttaagtgcc
     4501 atcaccttct gtggagattg tggcgatatt tatagacggg tctattggaa tatccacgga
     4561 agaaaagaat ttgtctggcg atgcgtgact agaatcgagc aaggtcctga agtttgtaag
     4621 aaccgaaccg taaaagaaga tgaactttat ggtgcagtaa tgactgcgat taataagcta
     4681 cttgcaggtg ggaacaatat gataaagaca ctggaagaga atattcatgc tgtgattggc
     4741 gaaaccacag aataccaaat ttcagagatt aacaactcac tagaggaaaa acaaaaagaa
     4801 ctaatcaagc tggcgaacaa gggtcaagac tatgaccact tagcagatga gattgatgag
     4861 ctgagagaca agcgacagat ccttttagta gaagatgcct ccctcagtgg cgagaacgag
     4921 cgaatcaatg agctgattga atttatccgc aagaacaaat tccgcacctt agaatatgat
     4981 gataagcttg taaggaagat aatccagagc gttacagtct atgaaggcca cttcgtcata
     5041 gccttcaaat ctggcatcga aatggaaata tgaaaaccag aagtaaatag cccatgactc
     5101 tacagtagag ttgtgggttt tcttttgctt gctgattata ataatatttt aataactatt
     5161 gtgtttatca accaaatggt ttataatatt cttagcgtaa acttggaggt gctgtatgac
     5221 tacggcagaa atgattaaag aactgtgtga gcaaatgaat ataagtgttt ccgaacttgc
     5281 tagacgtatt ggccagactc cacagaattt caataaaaaa ttacaacgag aaacagtaac
     5341 cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc aagtttgtgc aggcatttat
     5401 tttaccagat ggagaagaaa taaaaatatc taacgaataa aggaggatgt ttatatggta
     5461 ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt tgccttttat agaaggcatc
     5521 gtattagggg gctcacgtgc aagaggcacc catacagaaa attctgatat agatatcgga
     5581 atctattaca attcagactc atttgacctg acagcgatta atcaaattgc tacagagttg
     5641 gatgatgaga atagaaacaa ccttgttgta cctcccggag cgtggggtga ttgggttaat
     5701 ggcggtggat ggttagttat aaacgggtgt catgttgact tgatattacg tgatataaaa
     5761 cgggtggaac aaataatcaa agatacggag caaggaattg ttactgccaa ttatcagacc
     5821 gggcatcccc atggttatat aagtgctatg tatcgtggag aattagcgat tagtaaaata
     5881 ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc aggcagaaat ttaccctact
     5941 gctctaaaga aaagcttgat gaactttttt atatttgaag ctgaattctc tttaatgttt
     6001 gtaaaagcaa atgcgggggc agaggataaa tattatattg caggccatgt ttttcgtatt
     6061 atttcatgct taaatcaagt actatttgca tgtaataatg cttattgcat taacgaaaag
     6121 aaagctataa aactgcttga aacttttgaa tataaacctg aaaaatatgc cgaaagagtt
     6181 aatcatattt ttgaagtact cggtctttct ctttttgaat gctacgacat gaccgagaaa
     6241 ctttataaag aagtgaatga aattgtatcg gagataaata actttttaaa cgaggagagt
     6301 tcagatgaaa gaaaacaaat atgatgataa tatatttttt caaaaataca gtcaaatgag
     6361 tcgctcgcag aaaggactgg ctggtgcggg agaatgggag actttgaaaa agatgctacc
     6421 tgattttaag ggtaagcgtg tgcttgattt aggatgcggc tatggatggc actgtatata
     6481 tgcgatggaa aacggtgctt cctctgtagt aggtgttgat atttctcata aaatgctcga
     6541 agtagcaaaa ggaaaaaccc attttccaca gattgaatat gaatgctgtg ccatagaaga
     6601 tgtggatttc ccagaggaga gctttgatgt aatactaagt tcgcttgcgt ttcattatgt
     6661 agcagactat gagaatttaa taaaaaagat atataggatg ctgaaggctg gtggcaattt
     6721 agtttttaca gttgaacatc ctgtttttac tgctcatgga acacaagact ggtattataa
     6781 cgaaaaagga gaaatactgc atttcccggt ggacaattat tattatgagg gcaaacggac
     6841 agctatgttt ttggaagaaa aggttacaaa atatcataga acactgacca catatctaaa
     6901 tacactgctt tcaaatagtt ttataataaa tcagattgtg gagccacagc cgccagagaa
     6961 catgatggat attccgggga tggcggatga aatgcgacgc ccaatgatgc tgattgtatc
     7021 ggcaaaaaag aagatgtaat aatatagaaa aaataaacga ggagtatgta aatgagatca
     7081 gaaaaagaaa tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt
     7141 gtgacccttg aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat
     7201 gatattacat attttgtaag tgatatagaa ccgtttatat ctaatgatga ctggcttaat
     7261 caatttggga atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa
     7321 gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta
     7381 ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt
     7441 gataaagatt gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga
     7501 aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat
     7561 gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt
     7621 gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa
     7681 ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag
     7741 aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta
     7801 tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat
     7861 ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt
     7921 aaaaccggct gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga
     7981 ttacagaaat gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg
     8041 tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct
     8101 atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag
     8161 attgccttga atatattgac aatactgata agataatata tctttactac caagacgata
     8221 aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat atagaagata
     8281 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
     8341 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
     8401 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
     8461 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     8521 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     8581 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     8641 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     8701 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     8761 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     8821 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     8881 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     8941 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     9001 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     9061 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     9121 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     9181 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     9241 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     9301 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     9361 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     9421 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     9481 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat agagttctat
     9541 attagagatg taaagagttt tggtgaagag gtttgtgagt atggctttta taaccagggt
     9601 gcaatgagaa gcactaacag cagctagctt aagaacgcat aggatatact ttttatggaa
     9661 gtcacagagg aggaataaaa tttgaacagg attaacagag taacctctat tcttattcag
     9721 ctgcaatcaa aaaaaataat tcctgccaaa gaaattgcac agcgatttaa tataagctta
     9781 aggacagttt acagagatat ccggacactt gaagaagccg gaataccaat tggatctgag
     9841 gccggaaaag gatattttct tgtagagggc ttcctacttc cgccggtaat gtttacagcg
     9901 gcggaagtgg gtgcattgat tacagcaggg aaatttttaa attgccatgg agatgaatca
     9961 tttataaagg attttgattc agctatgtat aaaatcaaat ctattttgaa gcatggtgaa
    10021 aaaaactatg cacaggagtt ggagaacagt attaacgtgt acagcacatc tggacagaaa
    10081 aatacattgg cggataacgt tattgcagca attcagactg caatctgcaa taaaagggta
    10141 atatcaattc aatatcctgc atcaggaggg caagaaccgg aaagcagaat gatagagcct
    10201 atatcactgg ggttttatga acagaactgg tacttaatcg gttttgcagg ctaagaaatg
    10261 aatataggaa ttttcgtgtg gatcggatta aaagcatttg catagaagaa gagctctgtc
    10321 aaagccatga tggctctttg gaacaaatat taaaacaaat gctttcatat aaaaaactat
    10381 atgccctaca atgccgttgt aaaaattggg gaaaagggat atattgattt tatatctttc
    10441 tcaaaataaa tataaaagcc ctttaaagag ggcttttata tattaatcac aaatcactta
    10501 tcacaaatca caagtgatta atcacaaatc acttgtgatt tgtgattctt aatgatacaa
    10561 tattactata caaaaaaaga atggggcgta gttatggaga aggaaaaact aaaaatactt
    10621 gaagaattaa gacgtatttt aaacaataaa aatgaagcaa ttattatctt gaataattac
    10681 tttaaaggtg gtgttggaaa gtccaaatta tcgactatgt ttgcttactt gacagacaaa
    10741 ttgaatttaa aagttttaat gatcgataag gacttacagg caacattgac aaaagactta
    10801 gcaaaaactt ttgaggtaga attgccacgt gtcaattttt atgaaggctt gaaaaatgga
    10861 aacttggctt cttctattat tcatttgact gataatttag acttgatccc tggcacgttt
    10921 gatttgatgt tactgccaaa attaactcgc tcatggacgt ttgaaaatga aagtagattg
    10981 cttgctactc ttttagcacc tttaaaaagt gactatgatc ttattattat tgatactgta
    11041 ccaacgccaa gcgtttatac aaataatgca atcgtggcga gtgattacgt tatgatccct
    11101 ttacaagcag aagaagaaag tacaaacaac attcaaaact atatttccta tttgattgat
    11161 ttgcaagaac agtttaaccc tggactagat atgattggtt ttgttcctta tttagttgat
    11221 acggacagcg caacgataaa atcaaacctg gaagaactgt acaaagaaca taaagaggat
    11281 aatttggttt tccgaaatat tatcaagcga agtaataaag taagtacttg gtctaaaaat
    11341 ggcattacag aacacaaagg ctatgacaaa aaagttttgt ctatgtatga gaacgtattt
    11401 tttgaaatga ttgagcgaat cattcaatta gaaaatgaaa aagaatagaa tcacaaatca
    11461 caagtgatta atcacaaatc acttgtgatt tgtgataggt gatgataaaa taaatagtaa
    11521 gaagaaatag aaagaagtga gcgatcgtgg gaaatttagg cgcacagaaa gcaaaacgaa
    11581 atgatacgcc aatcagcgca aaaaaagata taatggggga taagacggtt cgtgttcgtg
    11641 ccgacttgca ccatatcata aaaatcgaaa cagcaaagaa tggcggaaac gtaaaagaag
    11701 ttatggatca agccttagaa gaatatatac ggaaatattt acctgacaaa ctttaaaagg
    11761 agtgaaaatg aaatggcagt tacgtatgaa aaaacatttg aaattgaaat cattaatgag
    11821 ttatcggcaa gtgtttataa tcgggtatta aattatgttt tgaatcatga actagatact
    11881 aaaaatactc gtttactaga agtgaatctt ttaaatcaat tagaagtggc acaagaagtt
    11941 gatttatttc aacaaccatt tgaagaatta caagctattc atgagtattg gcggtcaatg
    12001 aatcaatatt caaaacaaat tttgaataaa gagaaagtgg cttaacatgg cgaatattac
    12061 tgacttcacc gaaaagcaat ttgaagatcg tttagaaaag aatgttgaac gactaactaa
    12121 aaatagacta gcggttgaat cgccaaccgc ttttttactt ggtgggcaac cagggtcagg
    12181 gaaaaccagt ttgcgatcag caatttttga agaaacacaa gggaatgtta ttgtcattga
    12241 taatgatacc tttaaacaac agcacccaaa ttttgacgaa ttagtgaaac tctatgaaaa
    12301 agacgtagta aaacacgtta ccccttattc gaatcgcatg acagaagcac ttattagccg
    12361 tttaagcgat caaggctata atttagtgat tgaaggaaca ggacgaacaa cagacgttcc
    12421 cattcaaaca gccacaatgc ttcaatctaa aggttatgaa acaaaaatat acgtcatggc
    12481 agtaccgaaa attgaatcat acttaggaac gattgaacga tatgaaacca tgtatgcaga
    12541 tgatccaatg acagctaggg caacaccaaa acaagcgcat gatattgttg tcaaaaactt
    12601 accgaccaat ttagaaaccc ttcataaaac gggcttattt agcgatataa ggctttacaa
    12661 tagagaagga gtgaaactct attcaagctt agaaacgcct tccattagtc cgaaagagac
    12721 cttagaaaga gaattaaatc gtaaagtatc agggaaagaa attcaaccga ctttggagag
    12781 aatagagcaa aaaatggttc aaaatcaaca ccaagaaacc cctgaattta aagcaattca
    12841 acaaaaaatg gaaagcttgc agctacctac accaccaata cccaaaacac ctaaacttcc
    12901 aggtatttaa accttaaaaa aggaaaagag tagttaccaa aaaacggtaa ctactctttt
    12961 tttataaaaa catttcctca tgttggggtc ccgagcgcct acgaggaatt tgtatcgata
    13021 agaaatagat ttaaaaattt cgctgttttt ttctaatttc attttgaggt gaattaattg
    13081 gtagttatta gaggtgccct atacctaata atttatctac attcccttta gtaacgtgta
    13141 actttccaaa tttacaaaag cgactcatag aattatttcc tcccgttaaa taatagataa
    13201 ctattaaaaa tagacaatac ttgctcataa gtaacggtac ttaaattgtt tactttggcg
    13261 tgtttcattg cttgatgaaa ctgattttta gtaaacagtt gacgatattc tcgattgacc
    13321 cattttgaaa caaagtacgt atatagcttc caatatttat ctggaacatc tgtggtatgg
    13381 cgggtaagtt ttattaagac actgtttact tttggtttag gatgaaagca ttccgctggc
    13441 agcttaagca attgctgaat cgagacttga gtgtgcaaga gcaaccctag tgttcggtga
    13501 atatccaagg tacgcttgta gaatccttct tcaacaatca gatagatgtc agacgcatgg
    13561 ctttcaaaaa ccactttttt aataatttgt gtgcttaaat ggtaaggaat attcccaaca
    13621 attttatacc tctgtttgtt agggaattga aactgtagaa tatcttggtg aattaaagtg
    13681 acacgagtgt tcagttttaa tttttctgac gataagttga atagatgact gtctaattca
    13741 atagacgtta cctgtttact tattttagcc agtttcgtcg ttaaatgccc tttacctgtt
    13801 ccaatttcgt aaacggtatc ggtttctttt aaattcaatt gttttattat ttggttgagt
    13861 actttttcac tcgttaaaaa gttttgagaa tattttatat ttttgttcat gtaatcactc
    13921 ctgaagtgat tacatctata aataaataca gaagttaaac gatttgtttg taattttagt
    13981 tatctgttta aaaagtcata agattagtca ctggtaggaa ttaatctaac gtatttattt
    14041 atctgcgtaa tcactgtttt tagtctgttt caaaacggta gatgttttat ctacattacg
    14101 catttggaat accaacatga cgaatccctc cttcttaatt acaaattttt agcatctaat
    14161 ttaacttcaa ttcctattat acaaaattt
//
LOCUS       DAZUMT010000074        14129 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000074 DAZUMT010000000
VERSION     DAZUMT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.074"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG8FM_002017"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG8FM_002017"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS5094665.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG8FM_002018"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG8FM_002018"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5094666.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACG8FM_002019"
     CDS             8118..9086
                     /locus_tag="ACG8FM_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5094667.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACG8FM_002020"
     CDS             9104..10009
                     /locus_tag="ACG8FM_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5094668.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACG8FM_002021"
     CDS             10006..10812
                     /locus_tag="ACG8FM_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094669.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACG8FM_002022"
     CDS             10969..11937
                     /locus_tag="ACG8FM_002022"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS5094670.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACG8FM_002023"
     CDS             complement(12254..12778)
                     /locus_tag="ACG8FM_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS5094671.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14129
                     /gene="scm"
                     /locus_tag="ACG8FM_002024"
     CDS             13065..>14129
                     /gene="scm"
                     /locus_tag="ACG8FM_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5094672.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4001 a         3048 c         2787 g         4293 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tcttttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 atgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatca
//
LOCUS       DAZUMT010000075        14082 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000075 DAZUMT010000000
VERSION     DAZUMT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.075"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..293
                     /locus_tag="ACG8FM_002025"
     CDS             <1..293
                     /locus_tag="ACG8FM_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094673.1"
                     /translation="PNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGG
                     PFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(560..1729)
                     /locus_tag="ACG8FM_002026"
     CDS             complement(560..1729)
                     /locus_tag="ACG8FM_002026"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5094674.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1933..3108
                     /gene="mltG"
                     /locus_tag="ACG8FM_002027"
     CDS             1933..3108
                     /gene="mltG"
                     /locus_tag="ACG8FM_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS5094675.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3244..3723
                     /gene="greA"
                     /locus_tag="ACG8FM_002028"
     CDS             3244..3723
                     /gene="greA"
                     /locus_tag="ACG8FM_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS5094676.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3892..4623
                     /gene="liaF"
                     /locus_tag="ACG8FM_002029"
     CDS             3892..4623
                     /gene="liaF"
                     /locus_tag="ACG8FM_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS5094677.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4620..5687
                     /gene="liaS"
                     /locus_tag="ACG8FM_002030"
     CDS             4620..5687
                     /gene="liaS"
                     /locus_tag="ACG8FM_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS5094678.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5694..6326
                     /locus_tag="ACG8FM_002031"
     CDS             5694..6326
                     /locus_tag="ACG8FM_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5094679.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6466..7131
                     /locus_tag="ACG8FM_002032"
     CDS             6466..7131
                     /locus_tag="ACG8FM_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS5094680.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            7159..7452
                     /locus_tag="ACG8FM_002033"
     CDS             7159..7452
                     /locus_tag="ACG8FM_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS5094681.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7520..8467)
                     /locus_tag="ACG8FM_002034"
     CDS             complement(7520..8467)
                     /locus_tag="ACG8FM_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS5094682.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8645..9448)
                     /locus_tag="ACG8FM_002035"
     CDS             complement(8645..9448)
                     /locus_tag="ACG8FM_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS5094683.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9594..10508
                     /locus_tag="ACG8FM_002036"
     CDS             9594..10508
                     /locus_tag="ACG8FM_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5094684.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10556..11026
                     /locus_tag="ACG8FM_002037"
     CDS             10556..11026
                     /locus_tag="ACG8FM_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS5094685.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            11111..11719
                     /locus_tag="ACG8FM_002038"
     CDS             11111..11719
                     /locus_tag="ACG8FM_002038"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS5094686.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11853..12053
                     /locus_tag="ACG8FM_002039"
     CDS             11853..12053
                     /locus_tag="ACG8FM_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5094687.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            12807..14033
                     /locus_tag="ACG8FM_002040"
     CDS             12807..14033
                     /locus_tag="ACG8FM_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5094688.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         4799 a         2148 c         2825 g         4310 t
ORIGIN      
        1 ctccaaataa agatgaaatt acatttacgt ttaaatcttc aaaaggcaaa acaatagttt
       61 tacctgtatt ccggtataaa ggattaacgg ttactgttaa ttcaaaaaat gctacagtac
      121 taaaacaagg aggtcctttt gttacagtaa aaagtgaaag tggaataaat aaagtaacaa
      181 tcaggtattc atacacattt ttttctaaaa tctcattatt tatttctata atcacgctaa
      241 ttgttcttct tttttattgc ataaaattaa aaataagaac aagccatgaa tgatttgcta
      301 gcacatattt tagcaatcta aagatactat tttagattta ataggccctt tgtcaaataa
      361 tgttggtaaa gagaaatctt agaataattt ggacagaaca gaaagaagaa cacctctttc
      421 tgttctgttt atttggaatt aaagtctgta gcaaaaattg atttttagat ttgtcccaca
      481 ctctttccta cgtttagtcg agtacttttt ttaagcaaat gctattcaaa gaagtagtat
      541 ttgctatctt ttcatcatat cagctatttg ccaacttagt agttccttcc atcaggtcat
      601 agatttggtt tcttagatca gcagtaggca gtttgatagt tttgtcagct tttatattgc
      661 tttccatgat gatagcatcc tgtgaatctt tgctaaatat agcagatgta tcaaaagagt
      721 cgatatttcc ttgcgacagg atataattat ctccgctata taccccacca ctatatggat
      781 gtccattcgc attttcacgc cataatgaga agagtaagtc tttggaaact agttttccag
      841 ataaaacttg tgtaaacagc gtatttaaat catagacgga catactgata ttaccagtcc
      901 ctaattcgtt atggatctct gtttcagatt ctgtcagtgg tttaaagtaa tcatcctctt
      961 tagacatttg ataggatagt gcatgattgg atgaattttt gacttggtca taaaattttg
     1021 tatgctgtaa tcctaaaggt tcgatgatat catcggtcaa taatttttca tacggttgat
     1081 ttgttaattt gatcaaaatt ccagtcaata tggtaaagtt caatgaatca taatgagagg
     1141 tctgataatc tgtaaatttt aagtgctgaa tcgcaaattg aacgacttct tcatcagtca
     1201 tcggtatggt tggagaagca gttcggtgca agcctgagcg catatacagc aagtcacgga
     1261 tagtaatttg ctggctgcca gcaatttgtg gatagaattg agccagctta gtatctaaag
     1321 aaaggtgccc acgctcgact tgttttaaaa tcaaaagagt agtcaatgct ttttgaaccg
     1381 aaccaatctg ataataaaga gaaggatcat ttggttgttg cgtgagttta ttcgcatagc
     1441 catagccttt ttccatgatg atgcgattat ttttctttac gtaaattgag ccaatataat
     1501 cattttttct taatagttga tcgatttttt caacgagctg ttgttcattg gtagtcagta
     1561 tcaacttttt ttgagctgat tgagcatggg caactatctg agagtgttca ttattgtaaa
     1621 attgccatcc aagaatagtt cctgtagaga acgcactaag gctaaacaac acgacaacga
     1681 tcattaaaaa tgaatatttt ctttttttag catgttttcc ttttttcata atagtttatt
     1741 tagtctcctt tcttttttac agaaagaata aagctaaaat agcttgtatc ttgataacat
     1801 ttattttaat cttatagtaa tggatttact acttacattt ttgatagaat atttaaataa
     1861 aatcattcac gccaattcaa atatcatgta taatgaataa agttacgaac ttgatagtaa
     1921 ggaggagttg ttttgtcagg aaacaaacaa cacccaagta aagataaaaa agaagccagt
     1981 aaggcagcta cagcagatat aatcgacaag cggagaaatc tgcgtaaaaa agaagataag
     2041 attgtgagga agatcatttt agtgattgct cttacattat tgattattgg cggatttctt
     2101 ggatttaccg tgtatcgtta tgtagatagc gggttgaagc ctttagataa atcagatgac
     2161 caattagtcc aagtcgagat cccgagtggc tcttctaaca agcagattgg tgagattttg
     2221 gagaaggaca atattattaa aagcgggatt gttttcaatt attatacaaa atttaaaaat
     2281 ttaactggct ttcaagctgg ctattatcag ttagcgccta atatgactct tgacgagatt
     2341 ggcaaacagt tgcaagaagg cggtacttct gagccaacaa aagttgcaga tgggaaaatt
     2401 gccattccag aagggtacga tatcgatcag attgctgaac gggtagcgaa ggtaactggt
     2461 aaagacaaga aagaattttt agatttagtg aatgatgaga ctttctttaa cgagttgcac
     2521 caaaaattcc cagaactgtt ggaaagtgca tctaaagctg agaacacaaa ataccgtttg
     2581 gaaggttact tattcccagc cacatatgat tattacacga gtacttcgct taaggatcta
     2641 gtgattgaaa tggtgaacaa gacgaatacg gtcatgcaaa actattattc agctatcaag
     2701 cagaaaaatt tgactgtaca agaagtttta actctagctt ctctagttga aaaagaaggc
     2761 gtaaaagaaa atgatcgtaa aaacattgca caagtcttct ttaaccgaat caaagcaaat
     2821 atgccacttc agtcagatat ctctgtttta tacgcattag gtgaacacaa agaattagtg
     2881 acgtatgaag atacagctgt agattcacca tataatttgt ataccaatac cggctatgga
     2941 cctggacctt ttgacaatcc aagcgaagag gcaatcaagg cagtattaga accagcagaa
     3001 aatgactatt attatttcgt agcagatacg agcactggaa atgtttattt cgctaaaact
     3061 tatgaagaac atatggaatt agtggaaaaa tatgtaaata attcttagaa aaacaggctc
     3121 tagacattga tcatttttag aaaaacattt acatttgaag aaaagcgtgg taatcttttt
     3181 tggactatat tcctcaaaag attacctttt gcggtatttt atcgaaaaaa ggagacgtta
     3241 cacatggtag agaaagttta tccaatgact ctagaaggta aagaaaaatt agaaaaagaa
     3301 ttagaagaac taaaaacagt caaacgcgga gaaatcattg aacgaatcaa aattgcccgc
     3361 ggtttcggag atctttccga aaactctgaa tatgaatcag cgaaagatga acaagcattt
     3421 gttgaaggaa gaatcacaac aattgaaaat atgattcgtt ttgctcaaat catcgataac
     3481 gacggtgtag atgcagatga ggtatcaatt ggtaaaacag ttacattcat cgaactacca
     3541 gatggagaag aagaagagta cacgattgtc ggaagcgctg aagcagatcc gttttctggc
     3601 aaaatttcca atgattctcc tattgcgcaa gcattgattg gcaaacgtaa aaatgatgaa
     3661 gtaactattg ctactccagg tggcgatatg ttagttaaaa tcgtcaaagt agaacaagcg
     3721 tagtctgact gaaagatcta gagagttcat cttactgatg atacactcta gatctttttc
     3781 tctttgtgta ttttagattt caacaaacga tcttacatct aaagtcctat agccaacagt
     3841 taaaaaaata ggtatgatga aatgagaatg tgtcaggagg gagaaagagc gatgaatagt
     3901 tcttggcgtt tttttgtggt agttgaagca ctactgctgt tatttgctgt ttggcaaatc
     3961 gtaaacaata ctggactttt gcttttagtg atattcggta ttttcaatat atatctagct
     4021 atgcgcaaac atcctcgtac taaatttcaa aattttcaat tgatattagg aagtttggtc
     4081 atttttttta gcttagtcaa cagtcctgct ttatggatga tggctgtatt tgccgtttta
     4141 tttattggtc tgaaaggtgt agagatttca gggatagatt taaccaaaaa tgctttttgg
     4201 cgaaagaaac agatcatgat gatccaaaca gagcaattaa aaacacataa taatgagcgg
     4261 aaaagacagc aattattcgg taaccaacgt attggaaacg acgtctatga atgggacgat
     4321 atcaacatcg ccgtcatttc tggtgatact atcatagatt tgggcaatac gcttttgcca
     4381 aaagatgata atattgtgat tgtaagaaaa gggataggca gaacacggat cttagtacct
     4441 ttgggcgtag ccatccgatt agagcatgcg acattagtag gaaatgtctt gtttgaagaa
     4501 gagcaatttt ctttgaaaaa tgaacaaatc aaaatctaca gcagtgatta tgatgaaaac
     4561 cctcgtagat tgaagattat tactaatacc ttgattggag atgtcgaggt gatacgaata
     4621 tgatgggaaa aatatccaga gcgatgctag ctgtttattc ggggattgcg gctttcctta
     4681 ttattctatt ttcacttttc acttattttt atgccagcaa tcaaagtcat tggtggggag
     4741 aattactgcg tgcacgttta ttatatgttc cgcttatctt ccatttgtta gccatttcct
     4801 taggtgtagg attaattgtc tttctattat tatcacttat tcaaaagaca aaatacggga
     4861 aaatcgaaga aaagctgcgt gcactttctt ctggcaatta tgaatccaaa ttgttgcttc
     4921 ttccgatccc aagtgcatcg gacgatttat acatcaaaga tattgataag gaaatcacta
     4981 agataaaaga aaaaatgatt gaagtatcta gtgaattaca aattgtaacg agccgtcccc
     5041 aatatgtaga tggacaaacc aaagaagaaa ttttagaatt agaaagacat cgattagccc
     5101 gtgagctgca tgattcagtt tcgcaacaat tgtttgcagc aatgatgatg atgtcggcat
     5161 tgacggagca agcagaaaaa agcgagacac cagaaatgtt tcgcaagcag ttgaaaatgg
     5221 tagcagagat catcaacgct tcccagtctg agatgcgtgc gctgcttctt catctacgtc
     5281 cagtcaattt agaagagaaa agtttaaagc aaggtatcga gcagttattg aaggaattgc
     5341 aaaataaaat ccagatttcg ctgaaatggg atgtagaaga tgtaaaacta tctagttcca
     5401 ttgaggatca cttgttccga atcgttcaag aattgttatc aaatacatta agacatgcta
     5461 aagccaatga attagaggta tatttgcaca aaatagacaa taatcttttg ttacgtatta
     5521 ttgacgacgg aacagggttt aatatgaatg aaacgaaaac gggaagttat ggattgaaca
     5581 atatcaaaga acgagtagct ggtattggtg gaacagtaaa aatcattagt tttaaaggtc
     5641 aaggtacaag tgtagaaatc aaggtccctt tgatgaagga ggcatagata aggatgataa
     5701 aagttttatt agtagatgac catgaaatgg tgcgcttagg cgtctctttt tatttatcta
     5761 ttcaagaaga cattgaagta attggagaag ctgaaaatgg acgacaaggc tatgagaagg
     5821 cgatgacact tcgaccggac gtcattttaa tggacttagt catggaagaa atggatggta
     5881 tcgaatcaac aaaagcaatc ttaaaagatt ggccagaagc taaaatcatt atcgtaacca
     5941 gttttattga tgacgaaaaa gtttatcctg ccattgaagc aggtgcagca gggtatttgt
     6001 tgaagacctc taccgcacat gaaattgctg atgctataag ggcaacgcaa cgtggcgagc
     6061 gagtattaga accagaagtg acaacaaaaa tgatggaaaa aatgagtcgg cgaaatgagc
     6121 ctgtattaca tgaagaacta acgaatcggg aaaatgaaat tttaatgctg atttctgaag
     6181 gaaaaagtaa tcaggaaata gcggacgaat tgtttattac actgaaaaca gtaaaaacac
     6241 atgtttccaa tatattggct aagctagaag tggaagaccg tacacaagcc gctatctatg
     6301 cattcaaaca tggtttggtg aaataaaaac gatgataaag aggataatca aaagctgaga
     6361 aaataactat tatttttctt ggctttttta caataatgat gcttgctttt gtttttgtaa
     6421 aggctttgtt atactgagaa aagaagatac taaaggagga aaacggtgaa acaaaatttt
     6481 gcaattgtag gtcttggacg atttggcgga agtatctgcc gaacactgat cgaagctggt
     6541 caagaagtac tggctattga tagtaatgaa gatcgggtca atgaatatat gaatatagcc
     6601 actcatgctg ttgtggcaaa tgcacaggat gaaatgacac ttcgatcatt aggcataaga
     6661 aatttcgatc atgtaattgt cgctattgga gaagatattc aagccagtat cttagttact
     6721 ctgatggtaa aggaaatggg cgtaccaaat attttagcaa aagcccaaaa tgagtatcat
     6781 gccagagtac ttgaaaagat cggagctgat cgcgtcgtcc atcccgagcg agacatggga
     6841 ataaggattg cgcataattt agtgtcaaag aatattttag attacctaga gttgtcagat
     6901 aaatattctt tagcagaaat ccgagtctcc aatcctaaat ttttcaacaa aacattagct
     6961 gaattaaatt tcaggcaacg ttttgactta acagtagtcg ccatacgaag agtagatcaa
     7021 acggttatcg cttctcctgc agcagatgaa tatgttcgtg aaaatgataa tctactggtt
     7081 attggagaga caggcgatgt ggatattcta gacgataaga tgaatcaata gaaagaaaag
     7141 agaaagtagg ttgttgggat gaaattaacg gtaacgccaa aagcacaaaa gtggtttgac
     7201 tcagaaattg atctgccaga aacttacgga atacgttttt ttggaaaagt ctacggcaaa
     7261 acagaggttc atgatggttt ttcgattgga atgtcagttg aacagccaga acatcctgta
     7321 aagaaagtgg aaattgatgg gacacttttt tttatcgagg aagcagatga atggttcttt
     7381 aaaggatatg atttacttgt tgattatgat cccaacctgg aggagccaac ctatcacttt
     7441 aaaaaacaat aaaaaaagag agggtgacac aatttttgtc accttctctt ttttcttttg
     7501 aaaatattgt tttcagctat taatatcctg cagacaattt tacttgattg gtttgaagct
     7561 catgggtggc aaagtagctt ttcagattgg ttaaaaaaat agccattaat ttcttttgaa
     7621 aatcaggtgt caaacctgca atgtgggaag taatcagtac attgtccatt ttccaaagag
     7681 gatgatcttg tggaagaggc tcttcctcaa aaacgtctaa tgctgcaaag gaaagttgct
     7741 ggttatttaa agcctgcacc agatcttgtg tcttaacaga agcaccacgg ccaacattga
     7801 tgaatacaga ttttggatca aagtgttcaa acatatgctg atcaaagagc ccagttgttt
     7861 cctccgttag aggtaagata ttgactacga aatctacttc aggaagaact tcctttagtt
     7921 gatttaaaga aaaagtcttt tccattcctt ctgctggatg accggatgta ttgattccat
     7981 agcagttcat gtctaatgct ttgcattttt ttgcaatcat ttttccaata tggccagttc
     8041 caatgattag tatttttttc ccttggatag attggtaatg gaccgatgaa cctaaccatt
     8101 tcatgtcact ctgtgccttt tgtgcttgaa aaagaccacg agaataccca aggatgattc
     8161 ctaagacatg ctccgtaatc gattcactgt ggatgccact accatttgaa agtaaaatat
     8221 gtttctttga aaaagtctca aggggtagct gatcaacacc agctgagata gattggaccc
     8281 atttcaaggt agaagaggct aatagttttt cttggtagtc tgagttccag cccaaactga
     8341 tctctacatt ttgtagatcc tctggttgta attctgtttt aaagatataa ccaggcgcta
     8401 tagtatggat ttcttcgata aattcatcac gaaatggacg atctgcataa atgattcttt
     8461 ctgacatagt taatttcccg cctttcttta ctctaagtgt agcgcataca agaagaactt
     8521 tgaagcgcct tgcttgtttt ttgagaaaga aatatttttc aaataaaaaa ggggctgtga
     8581 caaaaaaatg tcacaacctc taaaagggtc tattgaagta gaaataacga gttgtcttta
     8641 tttcttaatt gatcgatatt tttgcccatc ctttgacagc ttgagcaaaa ataaagattc
     8701 ctgcaaaagc aatgaaggcg ctagtgtcaa aagaaggaag aaagaccatc aagattgttg
     8761 caattatagt aggcaatgaa attgaataga tcaatgtttt taaacaatca agaaaagtag
     8821 cttttgttag tcgaaagcgt gcatagagaa aagcagcaat gttggtaaat agcaaagtga
     8881 tcagcagatt cagaaaacta ggataaatag aaaatataaa tgtcaggact tttatccaaa
     8941 gtggtataga tgcttcactt aaaaaagaac ggagctgctt acctgttaag ttttttaaag
     9001 attcattggt gtagggaagt tctaattggt tattgttcaa caaagcagaa gtcgtgcctg
     9061 tattaggaag agctatgata agtttgtttt ttaacatccc gacactaaca aaattcccca
     9121 tcaaatcagt agagatgtcc tgttctgttc gttttccctc tggatcaaat gtgaagataa
     9181 tggagtttgt ttggtaaatg aatccttctt tttcttcggt ttgaagctgc ccgttttgga
     9241 ttgtaaaatc aggaatgcga gtagcaatct tttgcccatc caattgaatg ttgtttaaga
     9301 cttgaaaaac ttgaaaagag attggcaaag ccataattat actcaaaact aaaagataga
     9361 caatggcttt ccaaaatggg acatttctcg cctcctttag gtctttgaat cgtataaagg
     9421 aactgataat taattgtttt gttgtcataa gtactcctca tctagtagtg tcatcacttg
     9481 caattctagc gaagtatcaa gtgaaataca agtgctttaa ggaaacagct gaatgattgg
     9541 tgaaatatca aaaaaatgtt attcttgtct aaagactatc caatataaat aaaatggagg
     9601 aaggaatagt gcctacacag ccacagagat catcaaaaaa actatttgga gtagtggttt
     9661 tgaatgcggt gatcacttta tcagaattta ttggagggat tatgtcaggg agtttgtctc
     9721 ttgtttcaga tgctttccat aaccttagtg atacattggc aattattttt tcttacgggg
     9781 cacaaaaaat ggcccgaaaa gaagcaaata aaaaacgtac gtatggctac cagcgtttag
     9841 aaatattgag tgctttcatc aactcattca tattgatcat attatccttg tttttaaccg
     9901 ttgaagcatt caagcggttc acttcaccag aaaagataaa tagtcatttg atgctaacag
     9961 tagcagttat tggattgctc gcgaatttgt tctcagtatt attattacga caagaagcag
    10021 atgaaagttt gaatataaaa tctagttatt tgcatttgtt aagtgataca ctgtcttcgg
    10081 taagtgtgat tatcggagca attttgatcc gttttttcga tatctactgg atcgatccag
    10141 ttattacact gatcatttcg atatatatat tgatagaagc gataatggtg atcaaaaaag
    10201 ctgccgctat tttgatacaa agtgcacctg ctatcgacta tgaaaaaatg gaacaagaaa
    10261 ttgaagcaat cgatggggta aaggatgttc atcatgtcca tatttggcag tatagcgaaa
    10321 agataattat tttcgacgga catattgatt ttgaagatca gctactaagc gagattgaaa
    10381 agatatatcc tcgtataatt tctttattaa aattaaaata tggtattaca catgtgacga
    10441 tccaagcaga gacaaaggca aaggatcaaa aaaaattaat ttttttaaat aaagatgaaa
    10501 gttattgaaa tatagagtat gataaataag aatttttaat aaggtaggtt ggctcatgaa
    10561 gcagtatcga cagttaaaag gatatttaga agcaaaagga tattgggaga tactagtcta
    10621 tctctttttt ggtggtttgg ctacgattgt aaattttgtt agttttgctc tcgcacgcca
    10681 atattttgat ttaagtatgg ccttatccaa ttctatttcg tggttttgtt ctgtattgtt
    10741 tgcttttgta acgaacaaac tttgggtatt ccattcaaaa tcaccgaatt ttacacatgc
    10801 acttattgaa tgcggtaagt tcttcttcta ccgtatcctt tcttacggat tggacatggg
    10861 ggcaatggta ttgttgatca atgttatgaa cagcaatgag tatgtggcga aaatcatcac
    10921 tcaaattatt gttatccttg cgaactatat tttcagcaag ttgtttattt ttaaagaaac
    10981 tgaggttttt gaagaagaag taggtcaaag tgaaaaagac gactaggcat aacaattgaa
    11041 gaatttcctt ctctttgtta ctctttgtaa tgtagacatt attaagaaaa cattgaggag
    11101 gaattttttt atgacttaca cattaccaga tctaccttat gcatatgacg cattggaacc
    11161 ttatattgac gaagaaacga tgcatctgca tcatgataag catcacaata cttatgtgac
    11221 gaatttaaat tcagcaattg agaaataccc agaattaggc gaaaaaacaa tagaagaatt
    11281 attatctgat atggacgcta ttccaacaga tatcaagaca gctgtacgta acaatggtgg
    11341 cggacatgct aaccattcat ttttctggga aatcatggca ccaaatgctg gtggcgaacc
    11401 tacaggagaa ataaaagaag cgattaatga agcttttggt gatttttctt cttttaaaga
    11461 agaattcaaa aaagcagccg ctggacgatt tggttctgga tgggcttggc ttgtaatgga
    11521 aaatggaaaa ttagctatta cctctactgc aaatcaagat tctccattga tggaaggaaa
    11581 gacaccaatt ctaggtttgg acgtttggga acatgcttat tatttgaaat ataaaaatgt
    11641 ccgaccagat tatattgcag cattctggaa tgttattaat tgggatgaag taaacaaacg
    11701 tttcgaagca gcaaaataac catttcctcc gtcatatcag tcttaaaaat aatttggaaa
    11761 ataaaagagc gaaagcttgc tagcgttttc taaattgggt ataatgttct tgcaaggtta
    11821 cttgcaagaa cttttttgga ggatatacat ttatggaaca cggaacggtt aaatggttca
    11881 acaatgaaaa aggatttggg tttattacag tagacggcgg agatgacgta tttgttcatt
    11941 tctcagcaat tcaaggagac ggcttcaaga gcctagaaga aggtcaagaa gtagaattca
    12001 cgattgttga aggtgcacgc ggacctcaag ctgctgaagt gactaaatta taataatttg
    12061 ttgaataaag acacgacttc ttagagagtc gtgtcttttt gtctatttat gaagttttta
    12121 gattttttgg ctaagtaaat gcatttgttc acttgacatg tcctaactaa ttatcaagca
    12181 aggaacattt tcttcgttag taatggattt taaagaaatt tcagtttttg gatgttgaat
    12241 aaaagaccat ttgtgaaacc actagtatac atactcatta ccatgtcaat tctattactt
    12301 taaaagaaaa tcgttttact ttgaagagga gcatatctat ccattaaaag atgatggtaa
    12361 tgcaatcact agttcgagtc attttaagag ccatgcagtg agctggaaat aaaacgaata
    12421 gaagatgggt ttaacaaaag gaccagtcat aggaagaaat tgtcgtgaaa tcgaatgata
    12481 atctgaacgg taggcaaaga ctgttaaaaa gatgggaaag gtatgtgcga tcattttact
    12541 gcacgattat ttggaaaata ccttgttgtt cgctttttct ggaaaaaata cataaaaaaa
    12601 gataaaacga ttgcgaactg cgtgtattcg gtctttcgat ctttttcacg gtatggtatg
    12661 atagttaaga attttgagaa attttaaaaa ataataatcg ttacacaaaa atgtttttta
    12721 aagttttctt atgattaaaa agtgaatttg ctgattgaac agttatatga gacaatattt
    12781 tagaaaatgt gaaacagagg tgtattatga agcgatggca aaaagttcta ttaaccttgt
    12841 taggggtatt cgttttgctg attgccggat tatcagcata cggaatcaga tttatgggag
    12901 aagcgagcca aacagtaaac aaaatttcta aaaactctaa tcgaatatcc tccaaacgtt
    12961 ctgagagagt aagcattgat gacaaagaac ctttttcggt tttgctatta ggtttagata
    13021 ccggtggatt aggaagaacg gaacaaggac gttctgatac gatgatggta gtaacagtta
    13081 atccgaaaca aaaaaaatca acgatcatca gtttggaccg agatatttat acgaatatcg
    13141 taggttatgg aacggtagat aaattaaatc atgcctacgc atttggtggt gtcgaaatgg
    13201 ccatggattc aattgagcaa ctgctcgata ttcctattga tcattatgta acgatcaatt
    13261 tggatggcat ggaagatttg atcaatgcgg tcggtggcat caaagtgaat aataaaattg
    13321 actttacttt ggatggtgtc catgtaccag taggtgaaca aacattgaat ggagaaaaag
    13381 gtttagccta ttctcgaatg cgacacgaag atccagaagg cgatatcggt cgacaacgtc
    13441 gtcagcggga agtagtgacg aaaatcgtca ataaagtttt aagtttagat ggcgtaagca
    13501 attatagaaa aattttaaaa gcagttgaga acaatgtgac gacggactta gattgggatg
    13561 acatgcttga tgtagcgacg aactacaccc cggcgtttga aacaatcaaa caagaccagt
    13621 tgcaaggcga aggacaaatg atcaatgata tctactatca aattttagga aaaaatgatc
    13681 ttctttcgat ccaaaatgaa ctgaagaaac aattaaatat aaaaacaagc gatacattac
    13741 ctaatttgaa aaatgagaat gcttcaatga tgttctttga tgattcggaa gatggcgatt
    13801 cgacaaacgg tgatggatcg aatagtgaag ggtacaccga ttattcttcc tattcagaaa
    13861 acacatatga tcagtaccaa gatccgaata cctattatca gcaaacagaa gatacaagtc
    13921 agtactatac accaagtcaa gatactcaaa gttatgatcc ttacgctgga caggaacaaa
    13981 atggttatgg ttatgaccag aataatgagt atggaacaac aagtgggtac tagaaaaaaa
    14041 tagtttggtt aataaagagt gccacctaaa ttagacaaaa aa
//
LOCUS       DAZUMT010000076        14069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000076 DAZUMT010000000
VERSION     DAZUMT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.077"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..224
                     /locus_tag="ACG8FM_002041"
     CDS             <1..224
                     /locus_tag="ACG8FM_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094689.1"
                     /translation="RNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREE
                     EIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(305..874)
                     /gene="thiT"
                     /locus_tag="ACG8FM_002042"
     CDS             complement(305..874)
                     /gene="thiT"
                     /locus_tag="ACG8FM_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS5094690.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(940..1042)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1048..2019)
                     /locus_tag="ACG8FM_002043"
     CDS             complement(1048..2019)
                     /locus_tag="ACG8FM_002043"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5094691.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2113..3486)
                     /gene="glmU"
                     /locus_tag="ACG8FM_002044"
     CDS             complement(2113..3486)
                     /gene="glmU"
                     /locus_tag="ACG8FM_002044"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS5094692.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(3989..4807)
                     /gene="purR"
                     /locus_tag="ACG8FM_002045"
     CDS             complement(3989..4807)
                     /gene="purR"
                     /locus_tag="ACG8FM_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS5094693.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(4974..5798)
                     /locus_tag="ACG8FM_002046"
     CDS             complement(4974..5798)
                     /locus_tag="ACG8FM_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS5094694.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5771..6451)
                     /locus_tag="ACG8FM_002047"
     CDS             complement(5771..6451)
                     /locus_tag="ACG8FM_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5094695.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6474..7412)
                     /locus_tag="ACG8FM_002048"
     CDS             complement(6474..7412)
                     /locus_tag="ACG8FM_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5094696.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7657..8508)
                     /gene="ispE"
                     /locus_tag="ACG8FM_002049"
     CDS             complement(7657..8508)
                     /gene="ispE"
                     /locus_tag="ACG8FM_002049"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS5094697.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(8662..11235)
                     /gene="mprF"
                     /locus_tag="ACG8FM_002050"
     CDS             complement(8662..11235)
                     /gene="mprF"
                     /locus_tag="ACG8FM_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5094698.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11376..12182
                     /gene="proC"
                     /locus_tag="ACG8FM_002051"
     CDS             11376..12182
                     /gene="proC"
                     /locus_tag="ACG8FM_002051"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS5094699.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12261..13817)
                     /gene="rny"
                     /locus_tag="ACG8FM_002052"
     CDS             complement(12261..13817)
                     /gene="rny"
                     /locus_tag="ACG8FM_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS5094700.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         4074 a         3095 c         2438 g         4462 t
ORIGIN      
        1 tgcgtaattt gatgcaggaa gggatcattt tttcttttga tcaagttggt ttgatcgatg
       61 gtaaatatcg tatcccaatc tatgtgattg aagattatat ttggaaatta aaaggcaccc
      121 ggtatcgaga agaggaaatc atcgctactt taatctactc tgtcaccgat cgacactggc
      181 aattggtaga agatgccgat ctttcttatc tgccattgaa gtagatctat ttcttatcaa
      241 aaaataaaaa ggaaacaaga aactaggaca tgattctgtc tcaagtttct tgcttctttt
      301 ttatttacag ctcctgtcgt ttatctggca caaaaatcga aggagtcgtt tgttggatcc
      361 ctaataaaac gatgatcgtt acaatagctg tactcaaccc gctggcacca ttcattacga
      421 aagaaaagag ccaaggattc atcccccata gtgcatacgc tccccagaaa accactcctg
      481 caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa tgcaccaaac acgatgcttt
      541 ttctaatttg tttcgtatta tcgtctacat agcctttttg gacatttgaa gccatgagcc
      601 ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat caacacttga acgacactta
      661 aataataaac ttgtcccaac ggaaaatgta ggatgcccca aatgaaacct gccagcatcc
      721 ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg aatctgccct aatgaaatag
      781 aaaaactgct gccgatctgg atcggaatca acgacaacac catcgctaac gctgcaacaa
      841 tcgttccttc tacccaaact ctattttttc ccataaaaaa acttcctcct ttgaataaaa
      901 aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac agctgtttat tcacaattcc
      961 tacgctcgca taaccgatac aggtacgaag ggtttagaaa tagattcaat ctcagcccaa
     1021 ttggactccc ctttgtgagt ttatttatta tttatttttt gtttcaaaca atggactaac
     1081 tggtttgttt tcatggatac gtttgattgc atctcccatt agtccgccaa cacttacttc
     1141 atcgattttg tcgattttac gatcttctgg aagatagatc gagtctgtaa caactagacg
     1201 ctcgatagct gaatcttcta ttctttgtaa agcagggccg gataatactg gatgtgtaca
     1261 tgaagcatac acactagttg caccagcttc tttcaaagca ttggcagcca atgtgatcgt
     1321 acctgctgta tcgatcatat catcgatcag tacacaagtt ttgcctttga cttgtccaat
     1381 gatattcatt acttctgcaa catttgcttt tggacggcgt ttgtctatga tcgcaatcgg
     1441 agatttcaaa aattccgcta atttacgtgc ccgtgtgaca ccaccatggt ctggtgaaac
     1501 aacgacaaca tcatcgcctt tgattccttt ttctaagaaa taattggcaa taagtggcgc
     1561 acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg atttgaactg catgaaggtc
     1621 caaagtcagc attcttgttg caccagcttt ttgaatcatg tttgctacta atttcgctgt
     1681 gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca tagccataat aaggcatgac
     1741 tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca atcatgatca gcaactccat
     1801 caagttatca ttgactgggc tgctcgttga ttgaataaca tagacatgcg atccacgaat
     1861 actttcttca atgtttactt gaatttctcc atcactaaat tgagtaacag atgattttcc
     1921 taactcgacg ccgactgctg ctgcaatttt ctctgctaat gggcgattgg aatttaacgc
     1981 aaaaattttt aatcttgggt caaagtaatg ttttgacatg gggacctcca ctttcttttt
     2041 cacaactcta gaattctacc taataaatag tagtcatatt ttttaaataa atcaagtgat
     2101 tttgcaaaag tcttagttta gataagggag tttttttgca tatccttctt tattgacttg
     2161 tcttgctctc gcaattgcca gcgcatattc tggaatattg tctgtaattg ttgacccggc
     2221 agcaatcgaa ctgtttttag ctacctcgac aggtccgatg atattcgtgc tagaaccaat
     2281 aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt ccatcatagt tgacgaagac
     2341 aacaccacat ccgacattga tttcttctcc cagcgtagca tcaccaacat aagtcaagtg
     2401 accgacttta gttcctttgc cgatttgagc atttttcact tccacaaagt ttccgatatg
     2461 gacgttttcg ccaatttcag cttttggtcg taaatgtgca taaggtccca catctgcatg
     2521 tttcttgaca tggctgcttt caatgactga gttttcaatg acgacgtgat cctcaatcct
     2581 actgtctacg atttttgaat gtgacccgat gacacaatca gaaccaatga ctgtatttcc
     2641 ttggatttgt acacctgctt cgatcaatgt gtctggaccg atttctacgc ctgcatcgat
     2701 gtatgtcgtt gctgaatcga caaagcttac gccattgacc atatgcatct ggttaattcg
     2761 ctttctcatg atctcatttg ctttagctaa cgcgatccga tcattgactc ccattgattc
     2821 ttcaaaatca tccgtttgat aagcagcaac tgtatggcct tcttctttta agatttcgat
     2881 gatatctgtt agatagtatt caccttgagc attgtttgtt ccaactttgc ttaacgcatc
     2941 gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg atttcttgta caagagcttc
     3001 ttctggtgtt gcatcttttt gttccacgat tttttctacg atcccaatat ggtcacggat
     3061 gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc aagatcgttg cacttgcatt
     3121 ttttccttgg tgatattcaa aaagattgtt cagcgtttca gtagtcaata atggtgtatc
     3181 gccactgata actaacgttg ttcctttttt tccttccaag aaagaagcag cttgcaaaac
     3241 agcatgtcct gttcccaatt gttctgcttg taacgcatat ttactgcgtt ctcctagttg
     3301 tgctttaact tgttctgctc cgtgtccaac gatcgtaatc acttcatctg gtttcgtttc
     3361 actgacccga ttaatgatat gttctaccat aggtttacca gaaacgggat gtaaaacttt
     3421 atataatttt gacttcatgc gggtaccttt ccctgccgcc aaaataattg cataacgtgc
     3481 gtccaactgc gtcactccta aatatagttt gctgatttca gtttatccca gtatacccat
     3541 tttttcaaca aaacaagtga ttttctttta tcccagcgtt ttctggataa aaaaatagat
     3601 agaccataac gggcctatct atttggtaac cggtgctctc ctgccaactc ctcggtcaca
     3661 agtcacacta ctcataaaac atgaaaagct gttttttgaa atcgtttctt gtgacttaga
     3721 gttaaacggt aggcttacaa cctcttcatt aaacgttttt caatcagctg accttcggta
     3781 ttagctcaat aaacggcaaa atatgaggag caatttttcc gtttattgag ctaatactca
     3841 ggacaggacg ctgatttcac aacctctaga taaacggtgt tcaaaagcca gtttctcggt
     3901 attaagccaa atttatgaaa aaatgtggaa agccattttc acaaaatttt tcttactact
     3961 cgaaactaag cagctttttc acaacctctt attctgaaaa ataatttcct ggcacaaccg
     4021 taatattttt cgtttgagta tctacatctt tgacataaag cagtgacgta taatcatcga
     4081 tggcgcgatg accgttgaat tttgattcgg caaatacagt gatgccaaca agttctgatt
     4141 cgaattcttc aatcaagctc ttcattccgt tgactgttcc gccgcctttc atgaaatcat
     4201 ccactacaag gacacgtgaa ccacgtttca aacttctctt agacagttcc atcttttcaa
     4261 ttctttcaga agatccagac acatagttga cacttactgt tgatccttct gttatttttg
     4321 agtcccggcg tacaataacg aatggcgcat tcagataata ggacacagct tgggcaatag
     4381 gtacgccttt tgtggctaca gtcattacag catcgatttt ttcaccaagg tatttagaag
     4441 caatgattct tccaacttga cgcagcagtt caggttctcc taacaaatca gagagataaa
     4501 catatcctcc tggaagcaga cggtcttgtt ctgatagacg ttctgctaga gattcaatat
     4561 attttttcgc ctcttcatat gaaatttcag ggatgaacaa tacaccacct gcagcacccg
     4621 ggattgtttc caaggttcca taccctcgtt ctttaaatgt tttcttgatg attcccaaat
     4681 cttcactgat agaagattta gcagattcat aacgttcggc aaagcttgtt aaggaaatca
     4741 attcatgcgg gtgattcaat aaatactgtg tcatgtcaac tagacgttca ctgcgacgta
     4801 ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt ttacattata taagtttttc
     4861 ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag ttcggtattt attaaaataa
     4921 aaagaagcct ataaaaaatt tttcgttttt ataaggcttc ttttctttat aatttagtta
     4981 ttttttcttc tgaaggcaac caatattcgt ttgatgatat ttacaatcag gaacaaagca
     5041 ataaagacta atgtgattgt tgctcctgga ggtgtatcta gataaaacga gctagttagt
     5101 cctgacagca tccctagaaa tcctacacaa acactgatag caatcacgac attgaagctt
     5161 ttgcctaatc gcataccgat tgctgcggga agaaccatga ttgcggatat caaaagtgca
     5221 ccggcaatcg ggatcatcac agcaatagcc acaccagtaa tcacattgaa catcatcgac
     5281 atcaaatgaa cgggtagccc atccacgtgg gcggtatctt catcaaaagt cagaacatac
     5341 atcggccgtt tgaataaaaa gaacaatata ccgatggcaa cgaataagat tcctaaaaag
     5401 atgacttgct cccatgtaat ggtcacgatc gaaccaaaca gataggattg gatacttgtt
     5461 gctgaattcc ctccgctcaa attcatcaat actaacgcta gcgccaaacc accagccatt
     5521 aaaatggcaa tggagatttc tgaataggtt cggtagatcg ttcgcagata ctccaatatc
     5581 acagctgcta atacaacgac tagcatggtc gtaagtgtag gattaaggtt caaaaagaat
     5641 cctaacgcta cccctgctaa agagacatga gaaagcgtat cagccatcag cgattgtctt
     5701 ctgatcacta aaaacacacc tagcatcggg gcgattccgg caatgaagat agcggcaaga
     5761 aacgctcttc tcatgaattc atatgaaagc aacgccattg agaatcctcc tttctcacca
     5821 gtcggatctg tctgtccgca tactgtttga tatcttcatg atcatgggtg atcattagaa
     5881 ctgatttccc atggacatgc gcactatgtc ggagaagacg ataaaattca tttctggatt
     5941 cttcatccat acctgttgtt ggttcatcaa gaacaaataa gtcagggtca gtagcaaaaa
     6001 tacgcgctaa actgattcgc tgcttttgtc cgccagacaa ttcacctatt cgccgatttc
     6061 gcatatccca catccctaca gcttctaaag cacgctgtac atgttgatga tctttttcag
     6121 agagacgctt gaaccagcgg cctcttggat atcttcctga acggactaat tcgatcaccg
     6181 tactaggaaa acctgcattg aaagatgcaa cttgttgagg aatataaccg atacttaatt
     6241 tttttccttc ttgatttttc tcagcaatct ttatcattcc ttttgaaggt ttcagtaatc
     6301 ctaacgatgc tttcaccaag gttgacttgg ctgctccatt ttctccagtt aaaatgacaa
     6361 attcacccgc atctacatga taagagacat cttctagtac aggttcatca tcatagtaaa
     6421 aggtcaaatc agatatttcg atataacgca ccttacaacc tccattctaa aacttaattg
     6481 atactttctt ttaaagcttt cagatttttt tccatgacag aaatataatt ttcgcctgct
     6541 ttgatttgtt cattagtcaa gctttctaaa ggattcaaca cttctagctt cactcctgtt
     6601 tcattcgaca atgtctcagc taccttcgaa gaggcatttt cttcaaaata aatcactttg
     6661 atgttgtttt tcttcacaaa ttcttttagc tcagctaagc gacttggtgt gggttcctga
     6721 tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt attgattcgc taaataagca
     6781 aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat cttttagctc atcggagtat
     6841 ttttgatcca gtgcttcaag ctttttgata taagcatcgg cattcttcgt aaaagtttcc
     6901 tttttatctg gatatttttc gcctaattct tcagcaatcg ttctgacttc ttttatagct
     6961 aataccggat cgagccaaac atgtggatcg tattcatgtg aatgaccatg cccttcttct
     7021 gattcttcat gttcgtctgc ttcttgcgat tctaaacggt cgattccttt agctgcttcg
     7081 atcatcaagg tctgtttgct atccactgaa tcctttaacg aatccacaaa aaattccatg
     7141 tctgaactat tatacacgaa aacatctgca tcggaaattt ctgctctttc ttttgcactt
     7201 ggttcgaaat catgcggctc cgttccagca gggatcaaaa gtttcacgtt tccttcatcc
     7261 cctacgatct cttttgtaaa atcatacatc ggataaaaag tcgtaacgat cgtcaaatcc
     7321 tcttttttgt ctgcttcctt gttcgtattt ccacatgctg caaacagtaa agcacctatc
     7381 attaccgtag ctgtcaaaag atattttttc atttatatgc tcctttcaat gaacaaatcg
     7441 gaattttacg atttattaac gtgaactaat gtatcaaaat aaaaaaacac agtcaataga
     7501 aatccatcaa tttctgattt aagcatgtat attcttgatt aaatcgtaaa atggatgagt
     7561 tgctgctcat atctagccat ttcactacaa taaaaaactg aagaatacca ttttaggatt
     7621 cctcagtttt tcatcaccta ttttacttct tatattttat tgcagggttc gtactaaata
     7681 aacttcctca caaaaacctt ttaacccatt atatactctc tgcgctcttg aataatgttg
     7741 acacaaggca aaaacggttg gtccgcttcc actcatcaat gcagcatctg ctccgtactt
     7801 gatcatacgc tctttgattt gatggatgac tggatgccgc tttcccgtta tatcttctaa
     7861 actgtttccc atttttttga tcatctgctg ataatcttgc tctcggatgg cttcagcaag
     7921 tccgtggatg tcttgatgtt ggatggcttg cgtatctact tctctaaaca ctgtacgtgt
     7981 agagacactc attcttggtt tgaccaacac aacccagcac tgtggcatag aaggtaatac
     8041 ttggatttct tcccctttgc caccgacaaa tgccgtactt ccgtagacac agtaaggaac
     8101 atctgtaccg acttccaaac cgatttctgc cagttcttct aacgaacagc ccagattcca
     8161 taattggttg atccctctta gagcagctgc acagtctgta cttcctccgc ctaatccagc
     8221 tgctactgga atattcttct taacaaagat cttgacaccg gttttgatat tgtatttatt
     8281 ttttagcaat aaagcagctt gataaacatt gttccgttca tctaccggta aaaatgcttt
     8341 atttgtttct acaatgattt tgtcttctgt tatttcttca aaaaatagat gatctgccaa
     8401 gtctacgctc gacatcacca tttccaactc atgataccca tcttgccgtc tatataatac
     8461 atccagtcct aaatttatct tggctggcgc tttttcaatc aattccatcc tctcacccat
     8521 tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa ataaaagtat agcttatgtc
     8581 tgcctagtgg ttgattttgt catcgtttca acagctttca cagaattttc tcttcataat
     8641 ccaaacaagt tctagtgatt cttataattc tgtgagcact tcttctgcca aactcggaac
     8701 atttcctttt tcttttccta tcagactgag tagctgcatg gtagcaaatc cgaaattact
     8761 tcctttccta taagcaaaat aacgtccttc ccaatgatcg acatatttta atttcttttc
     8821 ataagcagac tggaaggaat agaaaaaatt accataatta tatacgatat gcatgacttt
     8881 ttcttgaaag aaagaaaaat ctgtttctcc aacattagct aatggagcta agcctagatt
     8941 ggcttccgag tagcccattt tctcgtatac aataaacaga ttggctaaca gatagtcagc
     9001 taattcctca gattctttag gtaagacccg caacaaatca tatcccgcct gcttcttcgt
     9061 catcggctga gcggtgatga agccaacgac tttttgatct ttacggacaa taccgacacc
     9121 gctagataat acatatgatt tatcaaatcg tcccacagag aagtattttt ctcgtgcacc
     9181 ttgaagccat ttttgtgaaa cttcatttac agcttgatgt aattcttccg gcagttgatc
     9241 gtaccaagta aaatggtatc cttctttcat caaacgttga tattcaagtt gctgactcag
     9301 ccgagacaag tcttctgttg ctagatcaac aatccctgct tcacctactt tcgtgaagtg
     9361 aaaacccaat tcatgaagca tcactacgta gctttctgat atcttataaa acgcaagctg
     9421 atagcctaaa caatctgctt gtttcataaa ctgattcgtt gctgcccgca tcattttagg
     9481 attgccaact ggatctccta aaacaaagca tttgtttcct ttgatctgat aaccaaaaac
     9541 gacttgatct tggtcgtctt cttggtaata aaaataactg taacctgaca agcgaagctg
     9601 atggcttgta gctgtgcctc cgtatgtttc caataaacgg tcaaaacggt ctttttcgta
     9661 ctcactgcca agcttcgtag tatcttcagc taaatattga taaagcgcca ctaaagctag
     9721 caatgagatg cccaaaccaa taagccctga gaaccacaca tcatctgaag gaaacaaaat
     9781 aaaatgattt cctgttattc gaccgactaa aaagcgtccg ctgtgatagc ctgcaattgc
     9841 gtaaacgata aacaaaaagc cgaacaagct agcatcaaag attatagctc cccaagaata
     9901 aacgaatttt gttcgataaa attcttttct ggataaccac acagctaaca gaatcaagac
     9961 atataccaat atcaactgcc aagataacgt acgtgtgatt gtatttaaaa taccaaaaga
    10021 tagtaaagca atcgtaggca gaaatgcctt tttgactttt actgaaagtg ctctagcaag
    10081 gccgagtaat aaaaagccaa ccaatagatt cactgtctga tctaaaaaat tgaatgagaa
    10141 cggcaataat acttgaaata gccgactgat tgcagacaga ttagtgattg ttgatagaag
    10201 taccatcatg atccccgcaa aatagacagc acttacgagc acgatatgcg caaccttttg
    10261 gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt ttgactcctg tatgtgtgat
    10321 gaacaataga agtcccgtca taaaaggtag aacataataa aacaaccgat aatataatag
    10381 ccaaactact gctacatctt gccgaacagg caattgactc attcctaaga tcatcaaaac
    10441 gtcaaaagtc cccattccac ctgggaccat cgtcagcatt cctatcaatg tagcaatcat
    10501 gaacatagga tagatcgaaa gcatcgaaat atctattctc atcaacttgc cgatgatcaa
    10561 aaataccgca agggctcccg tccattgtcc aaaggatgcc agtagcagac caagcaagcc
    10621 ttttggataa aactcgaaga acaatgtctt tctctttaga taagcaaaaa agaaaatgac
    10681 tggtgctaaa aaactaccgc cgaaaagcca gatccaatag cttcgaaaaa tactttctga
    10741 ctgaataaaa aagacatcaa taaatgtaag aaacgaccat acagacaaac cagtcagcat
    10801 aaatagtgcg acttttgata cagtggctaa aaccattttt ctatttgtct gctttccata
    10861 aaaactagcc cgcagacttg ctcctaccac accaccaaaa ccggcgagat tgttgatcgt
    10921 gtttgttacc cacgcagcaa gaagaaactc ttttctttcc atttttggtt ttccttgttt
    10981 ttctaagact ctgatcgtca cccaatcata caacaacatc ggagtgacac ccagcagtcc
    11041 agccagaatc atgagcagga tcgtcatgcg gctttgttgt cccatccttt gccaaatatc
    11101 ttgccaactc atgccattag caatatttgc gacttgattt gccacaaaga ctaacacgga
    11161 gccgaaaaaa atcagtttta aaatcagact atgttccttt atccattgca gcatcaagta
    11221 tcgtttattt ttcacatttt cccattccta acttgtatac ttttgtgtca taccccaatg
    11281 ttatcttatt tattttaatt tttccacttt agacctgttc tttgcttgcg atgatagaaa
    11341 aagaaagata tcctaatgct aaaggagagg ataacatgaa agtaggattt atcggcacag
    11401 ggaatatggc acaagctatt attgatggtt tattaaaaaa aggaacagtc tcgaaagaag
    11461 aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa atttgctgct gaaactggcg
    11521 tccatgcatg caaaagtaat caggaagttg catcacaatc agatatcgtt cttttagccg
    11581 tgaaaccaaa aatcattgaa aaagtattgg aagaactaag agaatatagc cagaaagtct
    11641 tattcatttc tattgcttca ggaaaatcat taacagattt agaaacagcg cttgaagatc
    11701 ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt cagcgtagga gcaggcgttt
    11761 ctgcgatttg caaaaatgaa gctgccacgg aggaacagct tcagcaagct acgatgattt
    11821 ttgaagcaat cggcagcgtc tatcctttag cagaatcaga tttcagtaca ttcattggcc
    11881 ttgctggcag ctcacccgca ttcgtttaca tgctgatcga tgcgatggga cggactggtg
    11941 tactgaatgg tttgtccaaa cagacagcca ctgagattgc tgcaaaagca attatgggtt
    12001 cttgtcaaaa attcctttca agtgataaaa atccatggga acttgtagac caagtttctt
    12061 ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga agaagcagga ttcatcccag
    12121 caatcatcaa aggaatcaat acgacgatcg agaaagatct tgagatgttg aataaaaaat
    12181 aatgatagca atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg
    12241 ctcttctttt gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg
    12301 attactttga tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt
    12361 actaaacgaa cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg
    12421 cgtccagctt gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc
    12481 agacttctta aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct
    12541 gctgagagcg catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct
    12601 ccatgatgag aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat
    12661 tccgcaccga tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca
    12721 tggagcaacc cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata
    12781 ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg
    12841 aatcgcagac gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca
    12901 aacgctgcct cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg
    12961 accatttctt cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc
    13021 atccgtgcga tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta
    13081 tcatcgatga ttaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct
    13141 cgtccaatga tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt
    13201 tctgaaactt gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg
    13261 cgatctgctt cttctttggc acgctgttca gattctttta ccattagagt taactcatga
    13321 ttcagctctt cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca
    13381 gcaatttttt ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc
    13441 tcttcaatta attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg
    13501 cgtttttcta acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc
    13561 tcttgagatt ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat
    13621 ttctgatttt cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt
    13681 tttgcactat cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga
    13741 cgtgactttg cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg
    13801 agaatgttga ataccataaa atccacctcc atactatctt tttcattttt atcatctata
    13861 cagcttctat gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca
    13921 aaaaacatgc acaactttat tctaatgttt gtaccccgta gtgtcaactt taaataaaca
    13981 aagaaacggc tttctactta ttcattaaac gatggttttt aaaataaatg acactagttt
    14041 catataggta aacctttaaa ttgagaaac
//
LOCUS       DAZUMT010000077        13827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000077 DAZUMT010000000
VERSION     DAZUMT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.078"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8FM_002053"
     CDS             168..362
                     /locus_tag="ACG8FM_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094701.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8FM_002054"
     CDS             631..930
                     /locus_tag="ACG8FM_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094702.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8FM_002055"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8FM_002055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5094703.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8FM_002056"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8FM_002056"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5094704.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8FM_002057"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8FM_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS5094705.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8FM_002058"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8FM_002058"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS5094706.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8FM_002059"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8FM_002059"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5094707.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8FM_002060"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8FM_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5094708.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8FM_002061"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8FM_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5094709.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8FM_002062"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8FM_002062"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS5094710.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8FM_002063"
     CDS             7521..8291
                     /locus_tag="ACG8FM_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5094711.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8FM_002064"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8FM_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094712.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="ACG8FM_002065"
     CDS             9959..11194
                     /locus_tag="ACG8FM_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5094713.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="ACG8FM_002066"
     CDS             complement(12193..12453)
                     /locus_tag="ACG8FM_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5094714.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="ACG8FM_002067"
     CDS             complement(12480..13781)
                     /locus_tag="ACG8FM_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5094715.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4447 a         2829 c         2176 g         4375 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgac
//
LOCUS       DAZUMT010000078        13770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000078 DAZUMT010000000
VERSION     DAZUMT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.079"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..2194
                     /gene="acm"
                     /locus_tag="ACG8FM_002068"
     CDS             29..2194
                     /gene="acm"
                     /locus_tag="ACG8FM_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS5094716.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2466..2951)
                     /locus_tag="ACG8FM_002069"
     CDS             complement(2466..2951)
                     /locus_tag="ACG8FM_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS5094717.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3041..3386
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3431..4072
                     /locus_tag="ACG8FM_002070"
     CDS             3431..4072
                     /locus_tag="ACG8FM_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5094718.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4091..4726
                     /locus_tag="ACG8FM_002071"
     CDS             4091..4726
                     /locus_tag="ACG8FM_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5094719.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4744..5559
                     /locus_tag="ACG8FM_002072"
     CDS             4744..5559
                     /locus_tag="ACG8FM_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5094720.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5878..7314
                     /locus_tag="ACG8FM_002073"
     CDS             5878..7314
                     /locus_tag="ACG8FM_002073"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5094721.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7503..9950
                     /locus_tag="ACG8FM_002074"
     CDS             7503..9950
                     /locus_tag="ACG8FM_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS5094722.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10180..11247
                     /gene="rlmN"
                     /locus_tag="ACG8FM_002075"
     CDS             10180..11247
                     /gene="rlmN"
                     /locus_tag="ACG8FM_002075"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS5094723.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11237..11443
                     /locus_tag="ACG8FM_002076"
     CDS             11237..11443
                     /locus_tag="ACG8FM_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094724.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11440..12543)
                     /locus_tag="ACG8FM_002077"
     CDS             complement(11440..12543)
                     /locus_tag="ACG8FM_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5094725.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13095..13643
                     /gene="aac(6')"
                     /locus_tag="ACG8FM_002078"
     CDS             13095..13643
                     /gene="aac(6')"
                     /locus_tag="ACG8FM_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5094726.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4715 a         2299 c         2915 g         3841 t
ORIGIN      
        1 tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat
       61 gttatttatt attactaata taacatcact gattcctgtt catgtctatg cggatgcagg
      121 cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg
      181 aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga
      241 ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga
      301 aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc
      361 gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt
      421 tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt
      481 gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc
      541 tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg
      601 gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga
      661 aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg
      721 tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag
      781 cgctacattt aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt
      841 tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat
      901 aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg
      961 agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt
     1021 tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt
     1081 gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt
     1141 gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt
     1201 aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa
     1261 attctccata aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa
     1321 ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat
     1381 ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac
     1441 aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga
     1501 tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt
     1561 cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt
     1621 catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc
     1681 gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag
     1741 tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga
     1801 aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta
     1861 taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag gtagccatat
     1921 aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc
     1981 tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat
     2041 tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac
     2101 aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc
     2161 tgggatattc ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttgttaaat
     2221 atgactgaaa tcaaaagaat aaaattccga tttataatcg aaaaaagaag catttttata
     2281 tatgcttctt ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc
     2341 gtattagttt tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct
     2401 gatatatttt gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc
     2461 tatcattaca aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag
     2521 tcggaaaaat ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct
     2581 tgacgatcaa tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat
     2641 gctaatggag tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa
     2701 acaaaagata agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt
     2761 tcatcaggca agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt
     2821 tttacttgcg gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga
     2881 tcgacttttt gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt
     2941 gcacgactca tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac
     3001 aaatgaaaaa taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag
     3061 agtagccgtt aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg
     3121 tgaaacacat cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt
     3181 tacagctgcg gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta
     3241 aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt
     3301 ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt
     3361 ggaaccacgt ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga
     3421 ggaaaagaag atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac
     3481 aacattgaaa ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc
     3541 attaggattg tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt
     3601 tatgcgggga acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat
     3661 tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc
     3721 ttattttgca gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc
     3781 agcgcaagtt ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt
     3841 gacaaagatt gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc
     3901 cttgatctat atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg
     3961 cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat
     4021 tctaacaatc gtaatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg
     4081 aggaagaaat atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat
     4141 caaagatttg aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg
     4201 tggcggtaaa accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga
     4261 attttattta gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt
     4321 aggagtcgta ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac
     4381 cttagcacca aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga
     4441 attagcggct tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg
     4501 cggacaaaaa cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc
     4561 ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat
     4621 cctatcattg aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc
     4681 agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata
     4741 gagatgaaaa aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc
     4801 tgttcgaaca aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc
     4861 gttataggtt tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc
     4921 acaggatttg atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac
     4981 tttcagccca ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg
     5041 atatggaacg gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct
     5101 tatatggaaa acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga
     5161 caaatgaata ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg
     5221 aaacaaccag aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt
     5281 tttaatgaag cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa
     5341 gtatacgcag actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa
     5401 ggttcttatg agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg
     5461 aaaaaaatca atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc
     5521 gataaatggt ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa
     5581 agacgtgtca cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact
     5641 cggagctgaa tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg
     5701 gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta
     5761 ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg
     5821 ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg
     5881 gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg
     5941 tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt
     6001 gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat
     6061 catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc
     6121 tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc
     6181 gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact
     6241 ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt
     6301 cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa
     6361 gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta
     6421 gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct
     6481 aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc
     6541 ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg
     6601 gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga
     6661 aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa
     6721 gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat
     6781 caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg
     6841 acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag
     6901 aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc
     6961 ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta
     7021 ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac
     7081 ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc
     7141 tattgcacct ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat
     7201 ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg atataagaaa
     7261 gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct ttagttcaca
     7321 tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta
     7381 gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa
     7441 caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat
     7501 aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc
     7561 aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg
     7621 ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga
     7681 tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga
     7741 ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac
     7801 tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg
     7861 gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc
     7921 aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact
     7981 tcttgattgc acagcctgga agttatttag cagcagtgct attactggca ggaggagcat
     8041 tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac
     8101 gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg
     8161 aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag
     8221 ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa
     8281 atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc
     8341 caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata
     8401 caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta
     8461 cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag
     8521 ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa
     8581 aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca
     8641 aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag
     8701 taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc
     8761 ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga
     8821 tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac
     8881 tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga
     8941 tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga
     9001 ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc
     9061 cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga
     9121 cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat
     9181 atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac
     9241 agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag
     9301 atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt
     9361 tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg
     9421 tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat
     9481 caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc
     9541 gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat
     9601 tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc
     9661 attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc
     9721 cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta
     9781 tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg
     9841 aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca
     9901 ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg
     9961 atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa
    10021 aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg
    10081 agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat
    10141 agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat
    10201 ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga
    10261 gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg
    10321 aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa
    10381 cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat
    10441 aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg
    10501 acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac
    10561 cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat
    10621 gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt
    10681 gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca
    10741 attggtgctc ggcacattac agtttccact agtggattag cacacaaaat caaggaattc
    10801 gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg
    10861 cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg
    10921 gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa
    10981 gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa
    11041 ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc
    11101 agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt
    11161 gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag
    11221 caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa
    11281 aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc
    11341 gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa
    11401 tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc
    11461 ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt
    11521 ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa
    11581 gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg
    11641 gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa
    11701 taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag
    11761 aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa
    11821 aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga
    11881 gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat
    11941 gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc
    12001 agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt
    12061 atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg
    12121 aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg
    12181 cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat
    12241 gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg
    12301 gacactttca tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca
    12361 gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt
    12421 tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc
    12481 atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact
    12541 catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct
    12601 gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg
    12661 gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg
    12721 aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt
    12781 tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc
    12841 aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag
    12901 ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta
    12961 taatttttta ttttataagt aagataaata atgaaagaat agagatactg attggtagct
    13021 aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac
    13081 aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc
    13141 agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag
    13201 aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt
    13261 tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat
    13321 tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag
    13381 aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc
    13441 atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca
    13501 tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag
    13561 gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta
    13621 ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga
    13681 aataagaagg aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc
    13741 gaagaagcta cttttggaac accgtgtacc
//
LOCUS       DAZUMT010000079        13732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000079 DAZUMT010000000
VERSION     DAZUMT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.080"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1345)
                     /locus_tag="ACG8FM_002079"
     CDS             complement(143..1345)
                     /locus_tag="ACG8FM_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5094727.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ACG8FM_002080"
     CDS             complement(1348..1542)
                     /locus_tag="ACG8FM_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5094728.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ACG8FM_002081"
     CDS             complement(1589..2437)
                     /locus_tag="ACG8FM_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS5094729.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ACG8FM_002082"
     CDS             complement(2642..2992)
                     /locus_tag="ACG8FM_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094730.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3170..4033
                     /locus_tag="ACG8FM_002083"
     CDS             3170..4033
                     /locus_tag="ACG8FM_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5094731.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ACG8FM_002084"
     CDS             4657..5649
                     /locus_tag="ACG8FM_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5094732.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ACG8FM_002085"
     CDS             5665..6549
                     /locus_tag="ACG8FM_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5094733.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ACG8FM_002086"
     CDS             6598..8205
                     /locus_tag="ACG8FM_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5094734.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ACG8FM_002087"
     CDS             8206..9690
                     /locus_tag="ACG8FM_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS5094735.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ACG8FM_002088"
     CDS             9867..10859
                     /locus_tag="ACG8FM_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5094736.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ACG8FM_002089"
     CDS             10911..11780
                     /locus_tag="ACG8FM_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5094737.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG8FM_002090"
     CDS             complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG8FM_002090"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5094738.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4214 a         2322 c         2832 g         4364 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tt
//
LOCUS       DAZUMT010000080        13684 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000080 DAZUMT010000000
VERSION     DAZUMT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.081"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..1510
                     /locus_tag="ACG8FM_002091"
     CDS             83..1510
                     /locus_tag="ACG8FM_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094739.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1602..1967)
                     /locus_tag="ACG8FM_002092"
     CDS             complement(1602..1967)
                     /locus_tag="ACG8FM_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5094740.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1964..2329)
                     /locus_tag="ACG8FM_002093"
     CDS             complement(1964..2329)
                     /locus_tag="ACG8FM_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5094741.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2521..2769)
                     /locus_tag="ACG8FM_002094"
     CDS             complement(2521..2769)
                     /locus_tag="ACG8FM_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094742.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2768..2896
                     /locus_tag="ACG8FM_002095"
     CDS             2768..2896
                     /locus_tag="ACG8FM_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS5094743.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2953..4647
                     /locus_tag="ACG8FM_002096"
     CDS             2953..4647
                     /locus_tag="ACG8FM_002096"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS5094744.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4747..5043
                     /locus_tag="ACG8FM_002097"
     CDS             4747..5043
                     /locus_tag="ACG8FM_002097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094745.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5421..6554
                     /locus_tag="ACG8FM_002098"
     CDS             5421..6554
                     /locus_tag="ACG8FM_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS5094746.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6585..6905
                     /locus_tag="ACG8FM_002099"
     CDS             6585..6905
                     /locus_tag="ACG8FM_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS5094747.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6934..7398)
                     /locus_tag="ACG8FM_002100"
     CDS             complement(6934..7398)
                     /locus_tag="ACG8FM_002100"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5094748.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7529..8782)
                     /locus_tag="ACG8FM_002101"
     CDS             complement(7529..8782)
                     /locus_tag="ACG8FM_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5094749.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8811..9128)
                     /locus_tag="ACG8FM_002102"
     CDS             complement(8811..9128)
                     /locus_tag="ACG8FM_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5094750.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9154..9474)
                     /locus_tag="ACG8FM_002103"
     CDS             complement(9154..9474)
                     /locus_tag="ACG8FM_002103"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5094751.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9938..11353)
                     /locus_tag="ACG8FM_002104"
     CDS             complement(9938..11353)
                     /locus_tag="ACG8FM_002104"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5094752.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11370..12686)
                     /locus_tag="ACG8FM_002105"
     CDS             complement(11370..12686)
                     /locus_tag="ACG8FM_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5094753.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12836..13534)
                     /locus_tag="ACG8FM_002106"
     CDS             complement(12836..13534)
                     /locus_tag="ACG8FM_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS5094754.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4348 a         2780 c         2355 g         4201 t
ORIGIN      
        1 aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat tgtgcaaaaa
       61 aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata ttttaagaca
      121 gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa agctctccaa
      181 tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa aaaaagaatt
      241 tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat atttttcaga
      301 acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt ttttaaaaac
      361 ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg aatgtgaatt
      421 catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt ttaatgactg
      481 cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac gtctgctgct
      541 tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg aatcgttttt
      601 tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat tttatgataa
      661 agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc agcaagtgtg
      721 tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta tttacaatta
      781 tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag aagaattcgc
      841 ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc agttgattgc
      901 ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa taaaagaact
      961 agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac cgttcgaacg
     1021 tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca tgccagaaaa
     1081 attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga caattttttc
     1141 cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca tgcgggcttt
     1201 tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta tctatatcgt
     1261 cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg taactacttt
     1321 cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga agtatgcgca
     1381 gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg cggtccaata
     1441 tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt cagctgtgca
     1501 aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt gaagatgccc
     1561 atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt ttggacatct
     1621 acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac taccaacgcc
     1681 gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat cccagctgaa
     1741 acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat agcattgttc
     1801 tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa aaaatccttg
     1861 attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag tcccagtaca
     1921 agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct acctctcctc
     1981 ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg actgttcgaa
     2041 tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata actgactcga
     2101 tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct agcagttttc
     2161 ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga gaccaatcaa
     2221 aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa caaatattta
     2281 aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata tacgctacct
     2341 actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt gttcgtctat
     2401 ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact ctcccatttt
     2461 tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc
     2521 ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg tacccaaatt
     2581 tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc tatttttatt
     2641 tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt ctcgtcctat
     2701 catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa acaaggaggc
     2761 gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg
     2821 cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt tatatgatga
     2881 attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt ggaagacatt
     2941 ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat tgccaatatg
     3001 gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat caccttttat
     3061 tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt ggatgaatat
     3121 tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg
     3181 cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc ttctgttact
     3241 gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca ccgaaccttt
     3301 gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa actgggattc
     3361 aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc agaagaagaa
     3421 aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt ttttgaaaca
     3481 aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc tgctcaagaa
     3541 ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca tctagtgggc
     3601 gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat cgagtacttg
     3661 aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga attcaaagaa
     3721 acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac actgactcaa
     3781 gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa tgggtccatt
     3841 acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat ggattatggt
     3901 gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg aaatcctgac
     3961 aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac
     4021 catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa tgccttgaat
     4081 tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa tatgtctatc
     4141 gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt tatttggggt
     4201 tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat taaaatgaat
     4261 ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc tggaccagtg
     4321 aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat ccctgtctat
     4381 atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt
     4441 cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc ttatcagatg
     4501 atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt
     4561 attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc tactctagct
     4621 cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc cctgcatcaa
     4681 gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt ggacttatta
     4741 ccgcagatgc aagcttttga acaccgttta ccaagaggtt gtgaaaaagc tgccctgcat
     4801 caagtaataa gaacacccaa gctaaaactg cttcttatgt ttttgacttg gttggactta
     4861 ttaccgcaga tgcaagcttt tgaacaccat ttaccaagag gttatgaaaa agctgccctg
     4921 catcaagtaa taagagcagc caagctaaaa cagcttctta tgtttttgac ttggttagac
     4981 ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt
     5041 tagtttcgag tatgaagaaa aattttgtga aaatggttcc tcacgttttt gcataaattt
     5101 gacttaatac cgagaaactg atcccttctg tcataacctc tttttattta tgtctgtttc
     5161 tttttgaaaa caactatttt ttcaacgttt actctcttgc caatccaaca tcatctaacg
     5221 gatacaacta tttcaatgat taccattaca ctaaaaaatc ggaaacacac ctctccgaac
     5281 acaaatacat aagactatag aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa
     5341 ggtaagatct cctttaaaat gaaattatct gtagtactaa taatgcagac aattttatga
     5401 atctgaaagg agcatctcca atgaaagcag tcacttggca aggaaaacag aaaatggaaa
     5461 tcagaaacgt agatgatcca aaaatattag aaccaacaga tgcaatcatc cgtatcacag
     5521 caacagccat ttgcggctcc gatctgcatc tttaccatca tggtgaagca gttttggaaa
     5581 aagggtatgt tgtaggacac gaaccaatgg gaatcgttga agaagtggga tctcaagttc
     5641 aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa tattagttgt ggtcattgtc
     5701 atttctgcct gaatcacatg gaaagccaat gtgatgaatc aaatccagat cagttatatg
     5761 gcgggctttt cggatttggc aagttgaacg gtaatcattg gggtggtcaa gcggaatatc
     5821 taagagttcc ttatgcagac tctacttcat ttgttgtacc agataatgat cttcctgatg
     5881 aaaaagtttt atttctatca gatattttgc ccacagctta ttggagcgtt gaaaacgcag
     5941 gagtaaaaaa aggcgataca gttgtgattc taggttctgg tccagtcggt ctattcgctc
     6001 aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc agtagatcca gtacagcacc
     6061 gtctagataa agcggctaat tacaatgggg ttgaaactta tttcttagaa gacacggcac
     6121 aagctggaac agaattgtat gaactggcta aaggtggcgc agatgtcatt atcgattgtg
     6181 taggaatgga cggattagag cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa
     6241 gcggcacaat ctctccatta cagatggcat cgcaagctgt caaaaaattt ggaacagtcc
     6301 aagtaactgg tgtttatatg actccagctt cttcttaccc gctgcaagaa ttttttatgc
     6361 gggatatcgc tgtcaagcat ggacaggcac caattatcca tctaatgcca aaaatttacg
     6421 caatgattgc agaaggacta ttcgatccta cacaaatcat tactcattcg ctgccactat
     6481 cagaagcagc acaagcatat gagatctttg acaaaaaaga agataaaaat ataaaagttg
     6541 ttttgaaacc ttgaattcag taaaaaatac aggtgatttt ctttatgccc aaaatcaaac
     6601 gaaataccca tgagatcgaa ataggaaaag aagaagatat cgaaatacga ggacgtcgct
     6661 tccgcttcta tttccaaaat cgttacacat tgatctcact ggctgtcgat ttattaaccg
     6721 ggattttcta tatttttgga agccttgctt ccttaactaa aatccccgat cgatatggca
     6781 cctattttta tttggctggt gctattttct tgacgattcg tcctattctc agaatcgtaa
     6841 ggaacatttt tatttacgac gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg
     6901 catgagaatt tttcaaatct catgcctctt ttttcaccct atcgtatcac tttgatacag
     6961 acgagcggtc gtcccacttt cttccttgac cattgtcaaa aatgtccact cgtatcgttc
     7021 ataaaacgtg gtatgatctg taattaagta aagtttttcg tacccaagtt gatgcatttc
     7081 ttttctgatt tccgctaata tccgcttggc tatcccctgc ttacgctttt ccggttcaac
     7141 aaacagggca caaagatttg gtgtcagatc agggcggtta tggaagtcgt tttcaatgac
     7201 gcccgctcct ccgacgatgg tttctttttc atctaagacc acataccatt gaggaatact
     7261 ttccttatta gcgattccag tacgaatact ttcctcatac acaacagcag gaactcccca
     7321 ttttgatgaa aaccaattcg cgctttgttt tactaattct gggtgttctc ttaatttgat
     7381 ccagttatat gtaaccaatg tttaaacctc cgactaaaat aaaacgttta caaaaattat
     7441 acaagtcttt atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa aacggaaaac
     7501 aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt cttctttctt
     7561 ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc aaattgacag
     7621 cggctagtac accacctgca atactttttg ttgccaatac accgccaata attggtggag
     7681 ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag
     7741 caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca
     7801 cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc gcaatcgaca
     7861 agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata gctaacagta
     7921 aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca
     7981 cgataaacgg tgcgctatgc ccagctgcga ttgcattaac gtttgcttca atggctggcg
     8041 cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca aagaaccata
     8101 agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca agtcccataa
     8161 atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg aatgcagcaa
     8221 aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt aatgaagcaa
     8281 atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat ttttcatttc
     8341 cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca ataaacaaac
     8401 cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa gaaatcacac
     8461 ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc aagaaagcaa
     8521 tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat cctttccatg
     8581 attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt aagttattga
     8641 tcattactgc taatgctcct aagatcatta acggcatact caccataaat gagtcccgaa
     8701 tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa tgtttctcca
     8761 taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga ttacgcgttc
     8821 aatcgacgat acagatcaat gatctctttt gctaaatctg taaaagctat agatgtcatc
     8881 agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct
     8941 tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc tgcatctttg
     9001 attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc ttccattgca
     9061 tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag atttttttga
     9121 tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt tttctgcttg
     9181 gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta catctaaagg
     9241 aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc
     9301 taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg aagcagatac
     9361 agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt ttgtgactag
     9421 gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt ccattttaaa
     9481 cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac tttaaaagcg
     9541 ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac acactaaaaa
     9601 agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt taacgtgcac
     9661 aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt attcaaaagc
     9721 ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt tttactttgc
     9781 tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac ggtgttcaat
     9841 cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta tttcaaataa
     9901 ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt tcgtcaggca
     9961 aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt ttcaatgttc
    10021 tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta aatgcattca
    10081 ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt gcgccttctt
    10141 ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg taatcatctt
    10201 gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata ccgttttctg
    10261 ttatcagcca agggatatta tgataatttt cttttagata catggctata tcatacatga
    10321 ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt aaatcaaaga
    10381 cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa cgaggggcgc
    10441 gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga tgctgaatcg
    10501 tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac aactccagga
    10561 actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta taaaacaaat
    10621 catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag accatttcag
    10681 gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc tctactgctt
    10741 tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg ttttgttcat
    10801 gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt tcgttgaatg
    10861 taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca tacttagcat
    10921 aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga aaagctggga
    10981 cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa gagtcgatca
    11041 tttcacttat atgctgagca tattcttcat ctactataca gttctcataa tcagtaaaaa
    11101 aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc atcagatcca
    11161 catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac ccattatgcc
    11221 aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct tctttttttc
    11281 ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa ttttttggaa
    11341 tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac acgatttgct
    11401 acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag ttgcgtaata
    11461 actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg cattggccaa
    11521 gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata accaatcgtt
    11581 ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag catgatcggc
    11641 ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga taacttggtg
    11701 atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa accgatcgtg
    11761 actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat aatgttcggt
    11821 acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa aagcaacggt
    11881 tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa gacttgcgca
    11941 ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa tgggcgttgt
    12001 acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat gataaatccc
    12061 gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa actagcagaa
    12121 acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt cctcaaccag
    12181 caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc tttcgtatct
    12241 gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt cactaattca
    12301 taaacagttg gattaacaga taaataagac atcactgcga tgataccagg gaatacacca
    12361 tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata gatggtcaag
    12421 aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc taaaaaacgc
    12481 aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga aaacaatgtg
    12541 ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt tatcaccatg
    12601 atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc taatttatcc
    12661 ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca tctctttttg
    12721 tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct tttttaccaa
    12781 atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg aactgtcaat
    12841 tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc atatggacag
    12901 tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg atctctgtat
    12961 gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta aacaaagcaa
    13021 ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta gcttctgcaa
    13081 tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct cgaaaagaag
    13141 tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt gcatattccg
    13201 ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag tctcgtatca
    13261 tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc cctcgcttat
    13321 cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa gctagagccc
    13381 gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat tcatattcat
    13441 tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg atcttgtcag
    13501 tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt tcatctgatg
    13561 tattttttcg taattattat aacacgtttg aatcatctga tgattttttg tatacaaaat
    13621 tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa aagactagga
    13681 taaa
//
LOCUS       DAZUMT010000081        13238 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000081 DAZUMT010000000
VERSION     DAZUMT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.082"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            173..1042
                     /locus_tag="ACG8FM_002107"
     CDS             173..1042
                     /locus_tag="ACG8FM_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5094755.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1251..2276
                     /locus_tag="ACG8FM_002108"
     CDS             1251..2276
                     /locus_tag="ACG8FM_002108"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5094756.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2315..3265
                     /gene="manA"
                     /locus_tag="ACG8FM_002109"
     CDS             2315..3265
                     /gene="manA"
                     /locus_tag="ACG8FM_002109"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS5094757.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3359..5062
                     /locus_tag="ACG8FM_002110"
     CDS             3359..5062
                     /locus_tag="ACG8FM_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5094758.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5064..7097
                     /locus_tag="ACG8FM_002111"
     CDS             5064..7097
                     /locus_tag="ACG8FM_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS5094759.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7108..7548
                     /locus_tag="ACG8FM_002112"
     CDS             7108..7548
                     /locus_tag="ACG8FM_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5094760.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7748..8875)
                     /locus_tag="ACG8FM_002113"
     CDS             complement(7748..8875)
                     /locus_tag="ACG8FM_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5094761.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8878..9555)
                     /locus_tag="ACG8FM_002114"
     CDS             complement(8878..9555)
                     /locus_tag="ACG8FM_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5094762.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9819..11585
                     /locus_tag="ACG8FM_002115"
     CDS             9819..11585
                     /locus_tag="ACG8FM_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS5094763.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11600..12037
                     /locus_tag="ACG8FM_002116"
     CDS             11600..12037
                     /locus_tag="ACG8FM_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5094764.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12050..13201
                     /locus_tag="ACG8FM_002117"
     CDS             12050..13201
                     /locus_tag="ACG8FM_002117"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS5094765.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4495 a         2301 c         2734 g         3708 t
ORIGIN      
        1 gcagaacagc tttttcacaa cctcttttta taagagtttt tactcattaa tttacaaaaa
       61 acaagcaaaa tgcgaatttt ctcacatttt ttttaacaaa atcaaagtta ttgcttgaaa
      121 gaagaagaat ccatcgttaa aataaagctt gacgtagagg ggagcaagaa atatgaaaga
      181 ttttcaattg ggggctgcca cctatttaaa aagtgtagcc attcgggtga aagaccgaga
      241 taaaatgatt gatttttaca agaacgttgt tggctttgat ttgaaaagag aagaaaacga
      301 attagccatt ttaggaacag aagaagctgg aagtgaaatg ttgctgctgg aagaaagtcc
      361 tagagcgaat gatcacatgg gggtgatcaa aaagctgaac cgtttttcat tgattatccc
      421 tagcacagaa gaaatggcag atatcctttg tcgtatccgt aaaatgaatt atccaatcaa
      481 agaagcttta gaagatcatg gacgtatggg gattttgctc gtagacccag aagagaatga
      541 attggaaatt taccatgaag agaataagaa cgaccaaaga gaaattccag aaacaatgga
      601 tcaagaagca ttattaagaa aatctgaaga aaaattcagt aaactatcaa caggttctta
      661 ttttgaaaaa atccatttaa atgttactaa cttaacaaaa gaacgccaat ttcttaatca
      721 tgtacttggt ttgaaggtac ataatgaaga agagggcatc catgtattga atgaaggaga
      781 tttccatgtt ggtttgacag aagctagcgg aggcgcaatt gatttgccta cagatgaagt
      841 gattggactt gattttctga aattcatgat cgatactgaa agcatggaag gactgattgc
      901 tcacttgcag caactatcag ttgatttctt cttagacaaa aagcacacag tactcacaat
      961 ctatgatgct ataggaatcg aatggtggtt cgtcagtgag ccaaaagaaa agaataaaac
     1021 tgacattcga tgcgacgctt aactttttct ttcttaacga ttgtcgcatg tctcgtcatt
     1081 ttatgaacgg tcatactatc gcttattcta gagatagaaa gagaaaatag tataccgggg
     1141 gaaactgcgc ggtttttcgc gcagtttttt tgtgtaaaaa aggttgattc ctcatagatt
     1201 aagctttaag cttggactgt aagaatctga tagaaaagca ggagaaaaag atgtatccac
     1261 acacgaaaca gcttattttg aaaaagatgg aagaaggatt ttatccaggt gtagcagtcg
     1321 ccttcataaa agaaaatcaa gtagaaacat ttacagccgg ctttgcccaa gtcgttcctg
     1381 aaaagcagct gatgacagaa gatctgttgt ttgatgcagc ctcactgacg aaagtcgttg
     1441 caaccacttc attagtttta cgtctattag aagatggaaa gatagaactc gatcaaccgc
     1501 ttcataaata tcttcctgaa tttgaaaatc agacaatcac tttgcgtcat ttactgacac
     1561 atacttctga tatcaaaaca tggataccaa acagagataa gctatctcag gaagaattga
     1621 tcgccgctta tttgaagcta gaaccaggtg agcaactagg aaaagaagta aaatatacag
     1681 atgccggtac gattcttctt gggttgatgc tagaggaaat ttaccaagaa actgcagtgg
     1741 aaatctttca agaacaagta ttggaaccat taggaatgat gaacagctta tttcttcctt
     1801 ttccttccaa aaaaatcgtt cccacagaac aactgccttc gggtgaggtt ttgcgtggac
     1861 aaactcatga tccgaaagca agagttttgg ctgaacatgc aggtaacgcg ggtctcttta
     1921 ttgatttgaa agacagcatt acttttgtcc aaatgtattt gaacaaggga aaaatccgta
     1981 atggtcgttt tttagaagaa gagacgattg attctttact gcaagatcaa acaccgaaaa
     2041 aaaatggcta tcgttcttta ggatgggact taaaaaagga tgtagaagac ggtcatgcga
     2101 ttctttttca tactggttat acaggtacgt ttttcttgat tgatataaaa acacaagaag
     2161 cgttgatatt tttgtcaaat cgcgtacatc ctgtcgatca tcgtgcggag tatctcatat
     2221 gtcgagatga aattgttgaa aactttttga aagagaaagc ggtattaccg aaataatgct
     2281 ataatagcta tgaaaaatga gaggagtttg aattatgcag gaacctatgt ttttaaaacc
     2341 agtttttcaa gaaaaaattt ggggaggaag tcgtttacgt tcagtctttg gttttgatat
     2401 ccctaacgat aaaatcggag aagattgggc gatcagtgcg catcctcatg gtgtcagtgt
     2461 agtagaaaat ggcgaattca aagggaaaag attagatgaa ctatggtcag aacacaagga
     2521 attgtttggc catccttcag aaccagtgtt tccattattg atcaagatct tagacgcaga
     2581 agatgatcta tctgtacaag tacatccaga tgatgcctat gggatgaaac atgaaggtga
     2641 attaggaaaa actgaatgct ggtatatcat cgatgctgaa cctggtgcag aaatcatcta
     2701 tggtcatcat gcaaagacaa gagaagaact agctgagatg atcaaagatg ggcgctggga
     2761 tgaccttttg aagaaagtac cagttaaaaa aggtgatttc ttttatgtac caagcggaac
     2821 gatccatgct attggtaaag gaatcatgat tttagaaaca cagcaaagtt ctgacacgac
     2881 ttaccgcgtg tatgactacg acagaaaaga tgcgcaagga caaacaagag agctgcatat
     2941 ccaacaatcg atcgatgtaa caactgtccc tgcgaaaaca ccagaattac aaatcaaaga
     3001 agtccgaaaa gggaattcat cgatcgtcac ttatttagaa actgagtttt tcaatgtata
     3061 tgagtgggat attaaaggaa tcacatcttt caaaaaacaa gccccttata cattgatgac
     3121 ggtaatcgaa ggagcaggag accttgtcgt tgacggaaaa acatatccat tagaaaaagg
     3181 aacaagctgt attattccaa atggcgtttc tgagtgggcg gtccaaggtg aactagcaat
     3241 cattgcgact gaaccaggca aatagttccc agatagctga aagcagcaga cggtttttcg
     3301 tttgctgctt tttctacata aaatccgtcc atcaataaat agaagtatgg tgaaaaagat
     3361 ggaatacaaa tggaaagcaa acaacagcag tcgcgtgtct atgaaacaac tgagaaaagc
     3421 tggacatcac ttaaatgaaa tgatcatgcc aaatctaagt ctgtttatcg cttggggaat
     3481 cttgtcatta gcaacaaaag gtgtgtctgg cgatttgcac accacattag aagaggtatg
     3541 ccattggatg atccagctgc ttttacccat cttgatcagt tattcaggcg ggaaatcggt
     3601 agggggacaa aaaggggcag tcgccggtgc aattgcttct ttagggttga tcgctcgttc
     3661 caatgctcca caaatcgtag gagcgatgat tatcgggccg cttgccggca tcgtatatga
     3721 acaatttgtt tctagattcc acgtgaaatt caaggctgga tatgaaatgc ttgtcagcaa
     3781 cctactagta ggactgactg gtagcgttat ttgtataatt ggaatcgttt ttattgaacc
     3841 agttttaaat agtgttcact tgttgctcgc ctccgttgtg tcttggctag tcacaatgaa
     3901 tctgttacca cttgtcagtc tgatcttaga acctctaaaa gtactatttt tcaataatgc
     3961 aatcaatcat ggtgtattga cacctttagg aatcgaattc tcacaaacag tcgggtactc
     4021 ttccttattt ttgatggagg caaatcctgg acctggttta gggatcttac tttctatcct
     4081 ttgttttgca gaaaaacagg aaaaagtaaa tgcgagtgga gcattgatga tccatgcgat
     4141 tggcgggatt cacgaaatct attttccatt tgtcttgctg cgaccgtatc tatttttagc
     4201 tgtaatggct ggtggggcaa gcggcacgtt gatatttcaa tggttagata cgggattagc
     4261 tgcacctgta tcgcctgggt cactactcgt gatttttgcc aatgcgcctt atccacaact
     4321 agctggggta agtataggga tagcaatcag tacatttgtg accttcattt gtgcagcgat
     4381 actattgaaa ttaccaaata aagaaaaaga gaacaaaacg gtcaaagaaa ggggagaaat
     4441 cttggaacca tctatcattc attccgtcat ttttgcttgc gactcaggta tgggatcaag
     4501 tgctatggga gccgccctgc tgaaaaagaa attagcagca gaaaaaatcg atctttctgt
     4561 cggatacagc tctatctatg aattgaataa ccaagtaaat cagctggtta tcgtgcaaca
     4621 tgaactagcg ggtcttgcaa gagaaaaagt acctcaaagt caaatctatg tactggatca
     4681 ctttttggaa acagatatct tgctgtcacg aataaaagaa gcgatccaat ttccaaagcg
     4741 ggaagaaaaa aaagagatag cagatatcac agaacaaaaa aagtcgcctg ctaatattgt
     4801 atttttgtat aaaaacaaca gacgcggctc acaaactatg ggcatgacga ttttaagtca
     4861 attagcaaaa aaagcgaaaa aacagtttgt tatctcaaaa gaatcaatag aaaaaatcaa
     4921 aatccaagaa gaaacgattt atatcgcaca aagagaacta gctgtaactg aacagctaga
     4981 agaaaagatc cctcggctag ttctagttga acattgggta gctacgaatg aatacgaaaa
     5041 actcgtgaag ggagaaatat aagatgtatt tatcgcccag agaaaaacaa ttattagcag
     5101 aatttctcag tagcccgtca cctgtatcta tccaaaaaat gatgaattta ttgaaggtca
     5161 gtaaacggac tatttaccgc gaattagata atcttgaagt ctcattgaaa tcggtaaatg
     5221 cttctttgaa aaaagcagct agaggctcat ttgtcattga ggcatcagaa gaagtattag
     5281 agcaattaaa agaaaataca gatgggaaaa gtaatgactt atccactgct aaacgacaac
     5341 acgcgatttt agccgagttg ttactgacga cagagccgtt gagtcttgct catttcatgg
     5401 aatcatatcg catcagtaat accacttttt atgcagatat caaacagttg gaagaaagta
     5461 ttcgccaact gcctttggaa atcattcgga accagggata cgagatcgaa ggaccggaaa
     5521 aataccgccg gcttctgaca gcgaatgtat tagaattaga aatcaatgaa tatgaacttt
     5581 ttcatagtat ttctttcgat gcatcactga attatttttt tcgattcgtt gatccgcaac
     5641 atctatctct tgcacgcaaa gtaatcgggg aagaattgat ccaaaaaaag accaatctaa
     5701 gtgatagaaa gcttgaacat ttggttttga tgctgacatt gacgatggag cgtgtgacta
     5761 aaaatcattt actgacaaat gagacatata atagtctggc aaacaaagaa ttactacaga
     5821 cagctaaacg attgttttca cggatcgctt ctgagacaaa acaactttat ccagtaaatg
     5881 agatcgtctt ttttgcagga ttattaaatg acttcacgaa ctcatttgat cagaatttct
     5941 ttgaagagac gttcgataca cagcttgctt atttagtaaa acaactgatt caagaagtaa
     6001 gtgaagagac gaatgtccat ttttatgaag atgaaacttt gtacaaaatg ctgttgactc
     6061 atttatctgg tgtgttttca agagccgttt tacaagaaga gacactgacc aatccgattt
     6121 tagaaaaaat catggttcaa tacgaggaag ttgcgaatgc aatccgtaaa gcagtagagc
     6181 ggatttttca agaaaaaaaa ttatccgaag aagagatcgc ttatatggtc cttcattttg
     6241 cgaattcatt ggaacgaagt ccgaaagaaa gcaatatcag tattgcagga ttttcgccaa
     6301 gtggattagc ttctactagc atgttggaac tacgattgaa aaggtatttt cccttcatcc
     6361 aacaaattca tttctatcgg attgctgatt taaaaaaaat cgatttacaa gaagaatatg
     6421 atgttgttat ttctacatct attttaccag gatacggtgg aaaatatatt ttagtctccc
     6481 cgctactgct agaagatgaa gtcaagcagc taaaagaagc tttttcgcat attgagaaga
     6541 aaaaaaggca aaaaattacg tataacagca ataacgagtg gatgatagaa gatacttatg
     6601 aggaaacaat gcatttcatg gatcaaatca atcaattact cggtcgtttt tttatccaag
     6661 aactggataa tcctgagacg atttccgaaa cgattcgcag cttatttcct tattttcctg
     6721 aaggactagt aacggatgaa gaggtggttc atcgccggct gcttaagcga tatgaacaag
     6781 cacctatcgg tattcctcat acaaatatgg ggttattcca tacatccagc tcattgattt
     6841 ctaaaccgat cttttgtatt ttcaatttga aacatccgtt gaccattgaa gggatggata
     6901 gacagccagt cgatttgtcc cgtatgttag ttatgctggc gccttcgcca atccaagaaa
     6961 aagacgggat tcttttagga aagatcagtg gagcaatcat catgaatgac ttgaacaccg
     7021 agatcttccg ctcaggaaat aaagaaatcg tttatcagtt gttagcgaaa atcctgattg
     7081 aagaaataaa gaaatgaagg tgaaaagatg atacagctac cagcagaaaa aatattgctc
     7141 gatcaatcct atcaaaattg gaaagaagcg ttgaccgaga tcggccagtg gatgacccaa
     7201 caagggggaa tcaaaccggc atatatccaa tcaatgatcg aaagacaaga aaaagcgagt
     7261 gtctatatcg ggaatttcgt agccttgcca catgggactg acaaagatga agaagtagta
     7321 gaagaaggaa tctatttagt ccaagtgcct gatggtgtgg actttggaac aaaagaggaa
     7381 ccaaaaatcg cgacactctt atttgtagtg gctttaaaaa aagaacatca gttagcggtg
     7441 ctccaagatt tagccttttt ctgttcagac atcgaccaag taatggcgct cagtgatgcg
     7501 aaaaccattg aagaagtcca aatgattttt gaacatggac atatataacc aatgttcaca
     7561 gtaaaaaaag ctgccttgca tcaagtaata acttcaacca agataaaaat aagaggagct
     7621 atttttgttg ggaggttctt attactgcgg atgcaagctt ttgaaaacta tttattctag
     7681 aaaaccgttt atttgaaata ggggacggtg acaaggtttt tgtcaccgcc cctattattt
     7741 ttgtgtttta atagctcttt tttatataga tagtgcccat acctatgaat aataaaacga
     7801 tactccatat accaagtgtc aagataccat tcatgaaagt aacctgttct gaagctagtg
     7861 gcgagaaaat atctcctcct atgatcacat tttgaaaatc aacataagaa gttggcaagt
     7921 aatgactgat tgattctatc catccaatcg aagaaaacaa gcgatactct gctaagaaaa
     7981 gacaaagaag tgtacctaaa agattgacta gagaatgttc aaataaagca gatagaagaa
     8041 aactgattgt acttaaaaag agcacccaca acaacaggaa cagaaagctc tggaaaagat
     8101 actcatacat cggaaggatt cgtatctcct gaccaatgat cgttcctaca ggataacgga
     8161 agtctcccaa accatttttg attattacta gtatagagac gatcaaaaat ggcatcacga
     8221 gacttagcat agctgcacca taataaatgg taaaacggat gctatgtttc tttaccatac
     8281 tatccggtaa tagattgact agagagatta cctgctttct tttttcataa gagatgatgg
     8341 aagaaagaaa caaggcggtt atacataaaa tcaaaaaact agaaatcatt cctgaaaaaa
     8401 tattttcgta gtaatttgct aaagggagat gcgctggcaa gtcaaaaata gaatatcttt
     8461 ggatctgatg ttctttcata taacggagca actcgacacg tttttgttgt tcaatgattg
     8521 gaattccttg tctttgacca gaaatcagtc gatccagtat atctttttca taatgataag
     8581 ttgcttcgac catttgctgg acattccctg tgttttttgc atgtaagatt gcttcgatca
     8641 gtttgttgct tttctctaaa ctttccacaa attctttctc attctcatct tctttcaacg
     8701 tttcaatcaa acgttgcatc tcttcatagt tatgattcag ttcatcgaat tctgtataag
     8761 caagtttatg atcaaaaaaa agtgggaatg caaagagcac gaccaacaat agaggaaata
     8821 aaaagtttac tttgtttttt agtaagagat tgcgttcaaa tgtaagtaaa gacatagtta
     8881 cacctcatat aatttacgat aaatagctaa aatctcttcc atatttgagg attctgtgac
     8941 gatttctcca ttttttagaa aacatgcttt agaacaaatc gattggatat tcgcgatagc
     9001 gtgggaagaa agaagaatcg tcttcccctc tttctgccat tgtctgatga ggctattcaa
     9061 gatatcaata cttcctgggt ctaatccgtt caacggttca tcgaataaca tgacagatga
     9121 atcactgatc aagtaagcag caagcaagac atgttgtttc attcctagag acatttttcc
     9181 aatcggcaaa gtaaggtact cagtcatctt caatcgttgg aaaacagttt ggcattctac
     9241 tttgctctgc caatagtatt tgatcaaagt caaataatca acagccgata gatagtcgga
     9301 taactgattc gttgattcta gaaaaaagaa agaccggcga taatctgtat aagcagtagg
     9361 agagatgttg tgaagggaca attgatcata agttgttggc agcagttggg caatgccatt
     9421 cagcaaagtt gttttgccaa atccgttcgg ggcaatcaaa gcagctactt ctcctgattc
     9481 tactaaaaga ttcaattgtc gaatcacacg ttttttacca cgtttcatgt tcatgttttt
     9541 gatcagtaat gtcatagata ggcctccaat ataaaattta ataacataat agaatggaaa
     9601 acatctaaat taaatgctaa caaaagttat tgtttgcata ttataaaaaa attaaaataa
     9661 cagttttatt taaaattgaa gttttctgat accgaaatct ttttattttg ctgacatact
     9721 caaacctttg gcacaaagtt tcgtgccaaa gtacgactat ttctcttttt agtaagcgga
     9781 tacaattaga gtatcaaaag gagagaggga gagacaaaat ggaacggacg attcccaaaa
     9841 acaacatttc gctaaaagcg agggtacaaa aattaggcag tactttatca agcatggtca
     9901 tgccaaatat tggagcactg attgcctggg gtgtattaac ggctctattt atcccagatg
     9961 gttatttacc aaatgaggct ttggctacca tggtaagccc aatgctaact tatctgattc
    10021 ctttattgat cggttatact ggcggaaaag taattgctgg tgaccgcgga tcagttgttg
    10081 gggcaattgc aacgatgggt gtgattgtcg gaacagatat tccaatgatg ctaggcgcga
    10141 tgatcatggg acctcttggt ggatatgcca tcaagaaatt tgatcagcta ttccaaaaac
    10201 ggatcaaatc cggttttgaa atgctagtca ataatttttc agcaggtctt atcggttttg
    10261 gattagcgtt attaggtttt tctgcgattg gtccagttgt cgatgcattg actcaagcaa
    10321 tggcaaaagg tgtagagatc attttatctg cccatttgat tccattgaca agtattttta
    10381 ttgaaccagc aaaaatctta tttttgaata atgcaatcaa tcatgggatt ttgacaccat
    10441 tgggaactga acaagtagca gaggctggaa aatctattct attcttgctt gaagcaaatc
    10501 ctggtccagg acttggcgta ttacttgctt ttactttgtt tggcaaagga gcagcaaaat
    10561 cttcagcacc aggagccatg atcattcact ttttaggcgg gatccatgaa atctattttc
    10621 cttacgttat gatgaaaccg ttattattct tatcagttat aggcggagga atgtcaggaa
    10681 gtttcatttt ccaattgatg gatgctggac taagagcgcc agcttctcca gggtcgatca
    10741 ttgcgattct agcgatgaca ccgttcaatt cttaccttcc tgttattttg ggcgtgattg
    10801 ttgccgcagc tgtttcgttt ggtatttctg cagtaatttt gaaagcggat gcgaaagata
    10861 caaatgaaga cttcgaaaaa tctgttcaag agacaaaggc agctaagcaa gcttccaaag
    10921 gtgcccaagg tattcctcaa ggagctacat tctctggcgt acaaaaaatc atttttgctt
    10981 gtgatgcagg aatgggatca agtgcgatgg gcgcttcgat cttgagaaaa aaagtacagg
    11041 aagcagactt gcctcaaacg gtgaccaatc aagcaatcag ccaactgaca gatgactcga
    11101 atacattgat cgttactcag gtcgaactac aagaaagagc aaaacaaaaa gcaccgagcg
    11161 cgcgttttgt ctcagtagaa aactttttga attcacctcg atatgatgaa atcattcaag
    11221 aattaagcag caagcagaaa acatcagttt ctgaaattga gaatgagcca gcagaagagt
    11281 ttctttcagc aaacatcaaa caaatcaaag agattctact tgtccacgat gatcgttccg
    11341 gatcagcaac aatggggatg accgtgctca aagatatatt agacaagaac catttaacta
    11401 tcccgttaag aaaagtcaat atccaagatt taaaagatga acctgattca ctgatcgtag
    11461 ccaaagaaag tttagcggaa aaagtacaga tggctgcacc tcaatccatt catttatcag
    11521 tcaacagcat ggtcaatccg caaaaatacg aatcgattgc acaatatttg aaacagacag
    11581 cttaagggag gaaaacagca tgcaaagttt gccattcgac atgatcgagc tcaatcgatc
    11641 ttttaaaacc aaagaagaag cgatccgcta ttgcggtcag atgcttgtag aagcagggtg
    11701 cgttgatgag gaatatatcg atgccatggt agatagagac aaaatgttat ctgtttatat
    11761 ggggaatttt atcgcgattc ctcatggaac agatgaagca aaaaaatatg tgaaaaaatc
    11821 cggcatctgt gtcgttcaag taccagatgg tgtagatttt ggtaccgaag aagaagaaaa
    11881 gattgcgact gttttgttcg gtattgcagg agtcggagat gagcatttgc aacttgtaca
    11941 gcaaatcgca ctttattgca gtgatatgga caatgttgtc cagctagcag acgcgctcag
    12001 caaagaagaa gtaacggaaa atctagcaat tgcttaagga gagagaaaaa tgagagcagt
    12061 acattttggt gcaggaaata tcggtcgagg atttattgga gaggttttgg cagctaatgg
    12121 atttgagatt actttcgtag atgtgaatca cacgatcatt gatgctttaa atgaaagaaa
    12181 agaatacgaa atcgaattag cagatgaatc aaaagaaaaa atcactgtaa aaaatgtcaa
    12241 aggaatcaac aatcaaacaa atccagaaaa agtaatccaa gaaatcgctg aagcagattt
    12301 agttactacc gccattggcc cgaatatctt accttttatt gctgagttga ttgcaaaagg
    12361 tatccaagaa agagaaaagg aaggaagcac aacatcttta gatatcattg cctgtgaaaa
    12421 tatgattggc ggttcagcat ttctagaaaa agaagtgtat aaatatcttc cggaaacatc
    12481 atttactgac aaatatatcg gctttccaaa tgcagcagtc gatcgaatcg tcccattaca
    12541 acaccatgaa gatcctttat tcgtccaagt ggaacctttc aaagaatggg tcattgacga
    12601 tagccaaaga aaaaataaaa aaatccaatt aaaaggggtt ttatatgtag atgatctaga
    12661 accatatatc gaacgcaaac tatttagtgt caatactggt catgcgactg tcgcttatac
    12721 aggtgccttg ttaggttacc agaccattga tgaagcaatg caagatgcgt tagttgtggc
    12781 acaattaaaa tcagttttgc aggaaacagg aagtttgctg attgccaaat gggggtttga
    12841 tgcagagcag catcatgcgt atattgaaaa aatcattcat cgcttccaaa acaaaaatat
    12901 ctctgatgcg atcacacgtg tcgctcggac accattacgt aagttaggtt atcaagaaag
    12961 atttacacgt ccagtgagag aactacaaga acataacttg acttgcccac atttgacggc
    13021 aacaatgggg attattttta attactatga tcctgaagat gaacaaagcc gtcaattgca
    13081 tgaaatgaag atccatgaaa acttggaaca attgattcaa gaagtcacag gaatcaatga
    13141 tccgaaaaca atcgggaata tcaagcaaaa cgtgaaccgt tatgccaagc aagtcgctta
    13201 aaataggata cgaaaaaggg gaatgtgacg aaattttt
//
LOCUS       DAZUMT010000082        13084 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000082 DAZUMT010000000
VERSION     DAZUMT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.083"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACG8FM_002118"
     CDS             complement(78..2198)
                     /locus_tag="ACG8FM_002118"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5094766.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACG8FM_002119"
     CDS             2428..3606
                     /locus_tag="ACG8FM_002119"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS5094767.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8FM_002120"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8FM_002120"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS5094768.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8FM_002121"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8FM_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS5094769.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACG8FM_002122"
     CDS             complement(4959..6212)
                     /locus_tag="ACG8FM_002122"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5094770.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8FM_002123"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8FM_002123"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS5094771.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACG8FM_002124"
     CDS             7789..9135
                     /locus_tag="ACG8FM_002124"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS5094772.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACG8FM_002125"
     CDS             complement(9443..9829)
                     /locus_tag="ACG8FM_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5094773.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACG8FM_002126"
     CDS             complement(10097..10246)
                     /locus_tag="ACG8FM_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094774.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="ACG8FM_002127"
     CDS             10708..10920
                     /locus_tag="ACG8FM_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094775.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="ACG8FM_002128"
     CDS             complement(11198..12997)
                     /locus_tag="ACG8FM_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS5094776.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaa
//
LOCUS       DAZUMT010000083        13063 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000083 DAZUMT010000000
VERSION     DAZUMT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.084"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      91..257
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            405..962
                     /locus_tag="ACG8FM_002129"
                     /pseudo
     CDS             405..962
                     /locus_tag="ACG8FM_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1020..3059
                     /locus_tag="ACG8FM_002130"
     CDS             1020..3059
                     /locus_tag="ACG8FM_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5094777.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3168..4319)
                     /locus_tag="ACG8FM_002131"
     CDS             complement(3168..4319)
                     /locus_tag="ACG8FM_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5094778.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4473..5003)
                     /locus_tag="ACG8FM_002132"
     CDS             complement(4473..5003)
                     /locus_tag="ACG8FM_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5094779.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5102..5452)
                     /locus_tag="ACG8FM_002133"
     CDS             complement(5102..5452)
                     /locus_tag="ACG8FM_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS5094780.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5609..7138)
                     /gene="ahpF"
                     /locus_tag="ACG8FM_002134"
     CDS             complement(5609..7138)
                     /gene="ahpF"
                     /locus_tag="ACG8FM_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS5094781.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7148..7711)
                     /gene="ahpC"
                     /locus_tag="ACG8FM_002135"
     CDS             complement(7148..7711)
                     /gene="ahpC"
                     /locus_tag="ACG8FM_002135"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS5094782.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7855..8517)
                     /locus_tag="ACG8FM_002136"
     CDS             complement(7855..8517)
                     /locus_tag="ACG8FM_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5094783.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8701..10347
                     /locus_tag="ACG8FM_002137"
     CDS             8701..10347
                     /locus_tag="ACG8FM_002137"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5094784.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10338..11420
                     /gene="uxuA"
                     /locus_tag="ACG8FM_002138"
     CDS             10338..11420
                     /gene="uxuA"
                     /locus_tag="ACG8FM_002138"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5094785.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11528..>13063)
                     /locus_tag="ACG8FM_002139"
     CDS             complement(11528..>13063)
                     /locus_tag="ACG8FM_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094786.1"
                     /translation="IKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHMSESNFL
                     LYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESVSYQILT
                     SLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSFSGKEVQ
                     IQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKLYFLLKI
                     CKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILARLSITK
                     IESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAFARNKLY
                     NGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQW
                     MVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCN
                     IVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQ
                     VTS"
BASE COUNT         4371 a         2390 c         2428 g         3874 t
ORIGIN      
        1 ttaaagattg ttaaatttca ctatttcatt tcaaattcca ttgcctaatc attaggaaaa
       61 gtctatcatg aactcgtaaa tagaatatag taataacctt gttaggtgag gctcctatac
      121 aaacataggc tgttgcccag aaacgtcgaa agtcgccaat gggtaaaaca gaaactgttg
      181 gattaaggct ttttccaata gctaagaatg ttttctttac gttgtatagt gctaaaactc
      241 ggacgatgag atgatgctag gtcagaattc ttgttcgaaa aggtttctga ccttttcttt
      301 atatttttgt gactgactta tttgatgaga caaactcgct cctcacctga caagtggatt
      361 gctgtgtgag gaggagatgc ggtgaaaaaa gatcgattgg tggaggtcgc tcggattcac
      421 ttctggttgc acaatacaac cagaaaacac aacaagcaaa actgatctct atcatgagag
      481 attcatatgt cgatattcca ggttacggaa tgaataagat aaatgcagct tactcttatg
      541 ggggagttga tttattaaat caaacattaa aggaaaattt caaatttgaa gctccctatt
      601 atgcaagtat cacatttcaa gattttatcg attgcgttaa tgaactgttt cctggtggag
      661 tcaagatcga tgcagaaaaa tctctagatt tagatggcgt atatataaac aaaggagagc
      721 aagtgatgga tggtaatact ttactccagt atgctcgatt tcgggaagac aaagaaggag
      781 acttcgggag gatcagaagg cagcagcaag tgatcaaagc tatctctcag cagctgaaag
      841 acgtaacttc aatattcaag ctacccaaag cggttgggaa attacttggt agtatccaaa
      901 cgaaccttcc agaaagtgtg cttctggatt gtggtatgga tttcctcaaa gacatatgtt
      961 agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag gaggaaaaaa
     1021 tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc ggcgcagtga
     1081 tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa gaaacccaag
     1141 cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac aagggagatt
     1201 ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca ttatctgaaa
     1261 aagagatctt agataaatac cagaatatat acggtgctgc tgatgtcaaa ggacttcaga
     1321 tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca tataaagcaa
     1381 agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca ttagacagaa
     1441 atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa atggaaggaa
     1501 atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata gatcgaaatg
     1561 gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca agtaaacttg
     1621 gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc gacttaacag
     1681 aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac tttgtcccat
     1741 tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt caagaagtag
     1801 acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac gtgggagata
     1861 tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa atcggtcgct
     1921 caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtggg aaattaagca
     1981 ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt caaaatggca
     2041 aagatatcaa attgacaatc gatgcgaagg cacaaaaaat agcttttgac agtctaggag
     2101 gaaaagctgc atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt gcgcttgcta
     2161 gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa gattacaaag
     2221 cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt tatgctcctg
     2281 gatctacgtt taaaatgatt acagcagcaa tcggtctcga caacggcact atcgatccaa
     2341 atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg ggatctttta
     2401 aaacaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct ttgatctatt
     2461 ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa tttcgtacag
     2521 gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg aatccagcac
     2581 aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact ggatatggac
     2641 agggcgaact tctaattact cctatccagc aagcagcaat gtattctgtt tttaccaaca
     2701 atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat aagaagaatg
     2761 tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt gtgcaagatg
     2821 taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg aaaactggta
     2881 cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc ttgtttgctt
     2941 tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg
     3001 attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat tatcaataaa
     3061 aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc cttttttcaa
     3121 ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca cacttcataa
     3181 actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa taaaatcacg
     3241 ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa
     3301 atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa tgaactatgt
     3361 tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt caaaatcgct
     3421 acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa atcaacttct
     3481 aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc ctctacattg
     3541 tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa aaaagcacca
     3601 atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca aataactagc
     3661 gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag taggaaaggc
     3721 gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca
     3781 agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct gaaacttaga
     3841 actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga tccttctttt
     3901 gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga agttgccgca
     3961 agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac
     4021 cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc aggtcggaaa
     4081 tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa taagatcaca
     4141 ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa tccagcttgg
     4201 cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa tctccgcgag
     4261 atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat aaattccatt
     4321 ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat tattgaaaaa
     4381 ataggaaaat atactcacaa aaaaaccaac aatccaaaag atttttttgg attgttggtt
     4441 tttatacggt ctattttaat aactaaaaat gattaacgta tagtcatttt tcttattctg
     4501 atgaaattga taatctcctg gatgcccatg aaaatcaaaa taaagccgaa tatttggaat
     4561 agaagcatca aacttttgaa tggattgaac aataagacga atccgccaat caacaacaga
     4621 atactactat aaacactcca acttttagct tttgtattca tgctgaagaa caactggaac
     4681 aagctgttga tgataacagc taatccaaga agaatcggca ggatagaaac gattgcaggt
     4741 gcaaaagcat agacgatcag agctaagatc aggaaaaaaa gaccactgaa cagaccgata
     4801 ctccaagaac ctgttttcct tttaaaacgg taagcctcaa aaagattcaa caacccaagc
     4861 aaaacaagat aggcggtgat cagataaccg ataaaatgaa aaacagcaga agggtttagc
     4921 acaatcgcaa tcccagaaat aatataaaca gcagctctta ataaggcgta tttttgaata
     4981 ttttgtttaa tcgtctcaaa catataagaa aacctccttt gacttatttt aacgtgaact
     5041 agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa
     5101 atcaacttct gaggatcttt tccatttttt tgccttttgc tagctcatcg atcattttat
     5161 ctaaataacg aatttctttc atcaagttat cctcgatgtc ttctactcgt acaccacaaa
     5221 caacacctgt aatcaaggaa cgtgaaggat tggggacagg ggcttccgca aaaaattgtt
     5281 cgaaagtcac ttgtttttgt agttgatatt ctagttctgt ttgggaataa cccgtcagcc
     5341 aacaaatgat ttcatctact tcctctttta tacgatcttt tctctccgct tttgctacat
     5401 acataggata aacgcttgca aaacttgtcg taaaaatacg attttgcgcc ataatcagtc
     5461 acctttcttt ttttcaaatt atttactact tatagtatat aagaaaaaga gaagaaaaag
     5521 cgattattca aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc cttttttccg
     5581 ctctctgact ttttacaatc ttcatagctc aagtgcgcat cagataatcg aatgctccta
     5641 aagctgcagt agctcctgaa cccattgaaa caatgatttg tttgtatgga ctatctgtac
     5701 aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg gtcactattt
     5761 catttcttgg attcatttcg atcgttccat ctagccaatc cgtattaggg agtaagccga
     5821 ctagaatgaa tactcctgaa acagctagtg tatgcatctc catcgtacgg cgatcgatat
     5881 aggagagaga ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa
     5941 cagtgacatt cgcaagagag actagtttat cctgtaagat ttgatctgct tttaattctg
     6001 gtaagaattc tagaacatat acatgttttg ctaacccagc caaatcgatt gctgcttcaa
     6061 caccagaatt accaccaccg ataacagcaa tctcttttcc tttaaataaa gggccgtcac
     6121 agtgtgggca ataagcaatt cctttatttt taaactcttt ttcgcctggt acattgatag
     6181 aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat ataccgttag
     6241 ctaagccaat attaataaaa tgttgctctt tatttaagga aacggcctgt tgattcatca
     6301 taatgtcgat agggtaacta cgcacatgac tttctacttg ctgcatcaat tttggacctt
     6361 ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt ttcaacgact tgtccgccga
     6421 aagaatcagc gacaagtcct gtacggattc ctttacgtgc tgcatagata gctgcactac
     6481 tagctgctgg tccaccgcca attactaata catcataagg ttctttttca aacaactctt
     6541 cttcaatcaa agggccagtg attttttcaa caatttgttc gatcgtcatg cgtccgctac
     6601 taaattcttc tccatttaaa aacacagttg gcactgccat gattttttta tcatcgattt
     6661 ctttttggaa catcccacct tcaatcatcg tatgagtaat atttggattt aatacagcta
     6721 atatattcaa tgcctgtaca acgtcggggc aattatgaca agtcaagcta acataactgt
     6781 caaaatgcaa tgtttgtgtt atttttttga ttttaagact caaagattca ctgattttcg
     6841 gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg
     6901 ggataccagc aaaaacgatt ccactttctt tgccaactgg attgatagaa aaactaggtg
     6961 tgcgcatcga atctcttttt tcccaagaga ttttaggaga catttctgct atttccttcg
     7021 taaattcagc tagtttttga ctattttcac tattatccaa actaacagaa aaaacaatag
     7081 gagattcaat cagtgctaaa tattgttcga gattttgttt agtttctttg tctaacatat
     7141 ccaccaccta aatctttcca acaagatcaa aacttggttt caatgtttcg ccactttctt
     7201 tccattttgc cggacaaact tcacctgggt gggtacgtac atattgagca gcgcgaatct
     7261 tatcaatcag tgcactagcg tcacgaccga ttccatctgc attgatttcc atagcttgaa
     7321 cgattccgtc tggatcaata ataaatgttc ctcgctgagc taatccttgc tcttcatcca
     7381 aaacatcaaa aatacgagaa atctgatgag acggatcacc gatcatcacg tattcgattt
     7441 taccgatagc atctgatgta tcatgccatg ctttgtgagt aaagtgggta tcggtagagc
     7501 aagaaaagac ttcaacacct aaagatttca atgtactgta ttgttcttgt aagtcttcta
     7561 attcagtagg acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata ctccagttcc
     7621 ccataagatc tttttcactg acttctaaaa attctccttg atgataagca tgtgcattaa
     7681 attcttcgat ttttttccca atcaatgaca tagtaaaacc tccaataata attatttatt
     7741 tataattatt gtaatttagt tggagttgca aagtcaatgg atattttgtt atttaataaa
     7801 gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa aatgctactg
     7861 ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca tcatgtgtaa
     7921 gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag ccttgagtaa
     7981 atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca ctcgtaacca
     8041 gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt cttttcttcc
     8101 tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag cgcgttcgtc
     8161 atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg
     8221 tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt ctcggacaaa
     8281 gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac tttgaggtat
     8341 cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc
     8401 gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt ctaagtcagc
     8461 atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc ttttcatctt
     8521 tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga aaaacaatct
     8581 ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg acatagcgat
     8641 ttttattgta taaaattcac ttgaatacaa aaactaatat gttagtttag gaggggaaaa
     8701 atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa agaagtagaa
     8761 atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc tgtctggatc
     8821 catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca acgtttagcg
     8881 gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga agaaataatt
     8941 gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc aagcagtgga
     9001 gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca tccagattgt
     9061 tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca attagttact
     9121 tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata tataggaccg
     9181 attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag tattcttgct
     9241 gcgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt ctctacagat
     9301 aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc aaaagaatgg
     9361 caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga agaaattgtt
     9421 gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc agttagcaaa
     9481 atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat
     9541 attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga tcattttccc
     9601 aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc aacggtagat
     9661 aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc tttagctatt
     9721 atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc agatttatcg
     9781 ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc
     9841 attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa tccaaatatt
     9901 cctgatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat tcgttatggt
     9961 gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact tgtattcata
    10021 ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca aggaaaacca
    10081 ttcatcccga gtccagaccc actattgaca gactttcagc ataagctttc cgatattcaa
    10141 ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt gtcaaataaa
    10201 gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga agaaatgttc
    10261 atcaagatgg tagcaggacc gaatgccgta cgaaaaacat tacaggatta cttacaagaa
    10321 catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag gaagaaaaga
    10381 ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc acattgatgg
    10441 atataccagt aggagaggta tggcccaggg agaggatcaa agaactaaaa gaagaaattg
    10501 agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa
    10561 ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc cgaaacttgg
    10621 cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac tgggtgaaat
    10681 ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata tctgatgata
    10741 ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat gaatttgaat
    10801 tgccgggatg ggaacctgag cgcttagctc atatcaaatc cttgcttgaa gcctatgcat
    10861 cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca atcatcccaa
    10921 catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca tactcgattt
    10981 ttggattacc tcgaatcgta aagaatcgag aagatttaga ttggttgtgc cgcatagtag
    11041 acagccctta taacggaatc actctttgta cagggagtat tgcagaagat ccagaaaatg
    11101 atgtatatgg gattttagca gaattttgtc aaagagatcg aatcccattt gcacatgttc
    11161 gaaatattaa atttttagct aatcgagatt tctatgaggc accgcataaa tccgaatacg
    11221 gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt aacgaataca
    11281 tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga tacggcttat
    11341 atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc gaaaaaggaa
    11401 acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg
    11461 ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt tatgtatctt
    11521 atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt gcttcttctt
    11581 ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct tctggtgtga
    11641 tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc aaagggcagt
    11701 cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc gattcatttg
    11761 aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca cgcgtatagt
    11821 tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt actaggtctt
    11881 caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt tgattttgtt
    11941 ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaac acgctatgtt
    12001 cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta agaaatccat
    12061 tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt tcaggtataa
    12121 acaaatcaaa tacagtacaa aaggactcca ccatttctct tgaataaaga acatgaggaa
    12181 cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat tcgattttcg
    12241 tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa attggcagat
    12301 taggtcgcaa aatatctaca gagggatcaa taaaaaagtc aatatattca tactctgtga
    12361 attctttttg tacgtctttc acagaagttg caggaaaatt ctctttacat atttttaata
    12421 gaaaatataa tttatctatc ataccgctat ttagtcggtc aagtacttct tttttaaaaa
    12481 aagtattcat cataaaagcc actacttgcg gatcttcatc ataaaattct gtagcagtac
    12541 ttgaaaagat gttccaataa atatctaatg ctgcatattg aatttgtacc tcttttccag
    12601 aaaaagactt tctgccatta ttagaaaagg agatagacaa accgtacaat gctaaaaatg
    12661 cattaatctt tttcattttc gtatacaaat aagaggcact atagtttgtt tgctgactta
    12721 aactgataat cgaatttgtc tgacagctga acaaagaagt caagatctga taactgactg
    12781 attcgttgca gtatcttcca aatagacgat aatagcagta agccggatca attccttcaa
    12841 aattcaactt taaaaaaggt ttttgcttat caatatgcag ttctgttaga ttgagacgct
    12901 caaagtcttg ttctaattct ttgatgtata gtaaaaaatt tgattcagac atatggaaat
    12961 gatctgctaa atattgtgta gtaataaaga aacgatgttc agataggaaa agaaaaatat
    13021 ctaattttgt attgcttttt ttcttcaaga gaaacgtttt aat
//
LOCUS       DAZUMT010000084        12458 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000084 DAZUMT010000000
VERSION     DAZUMT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.085"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..457)
                     /locus_tag="ACG8FM_002140"
     CDS             complement(143..457)
                     /locus_tag="ACG8FM_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094787.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            743..982
                     /locus_tag="ACG8FM_002141"
     CDS             743..982
                     /locus_tag="ACG8FM_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094788.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1344..1604)
                     /locus_tag="ACG8FM_002142"
     CDS             complement(1344..1604)
                     /locus_tag="ACG8FM_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094789.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1789..2286)
                     /locus_tag="ACG8FM_002143"
     CDS             complement(1789..2286)
                     /locus_tag="ACG8FM_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS5094790.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2416..3126)
                     /gene="budA"
                     /locus_tag="ACG8FM_002144"
     CDS             complement(2416..3126)
                     /gene="budA"
                     /locus_tag="ACG8FM_002144"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS5094791.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3139..4803)
                     /gene="alsS"
                     /locus_tag="ACG8FM_002145"
     CDS             complement(3139..4803)
                     /gene="alsS"
                     /locus_tag="ACG8FM_002145"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS5094792.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(5008..5670)
                     /locus_tag="ACG8FM_002146"
     CDS             complement(5008..5670)
                     /locus_tag="ACG8FM_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5094793.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5680..6495)
                     /locus_tag="ACG8FM_002147"
     CDS             complement(5680..6495)
                     /locus_tag="ACG8FM_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5094794.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6757..7200)
                     /locus_tag="ACG8FM_002148"
     CDS             complement(6757..7200)
                     /locus_tag="ACG8FM_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS5094795.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7334..7672)
                     /locus_tag="ACG8FM_002149"
     CDS             complement(7334..7672)
                     /locus_tag="ACG8FM_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5094796.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7660..8037)
                     /locus_tag="ACG8FM_002150"
     CDS             complement(7660..8037)
                     /locus_tag="ACG8FM_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5094797.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            8261..9454
                     /locus_tag="ACG8FM_002151"
     CDS             8261..9454
                     /locus_tag="ACG8FM_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5094798.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9620..10042)
                     /locus_tag="ACG8FM_002152"
     CDS             complement(9620..10042)
                     /locus_tag="ACG8FM_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094799.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10413..11087
                     /locus_tag="ACG8FM_002153"
     CDS             10413..11087
                     /locus_tag="ACG8FM_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094800.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            complement(11249..12421)
                     /locus_tag="ACG8FM_002154"
     CDS             complement(11249..12421)
                     /locus_tag="ACG8FM_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5094801.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         3826 a         2449 c         2046 g         4137 t
ORIGIN      
        1 taaactctag agaaattaat tcatcctaac ataaaataat tgttcactca aaagtttttc
       61 tagaacaatt aaaacagcag gcttcacatt cgctatacat ttgtaagaag taataattcc
      121 tgtactaaaa ggcataattg ctttaattat tttttgactt ctcttcaaac acaagaagta
      181 gttgtgtaat aattgcagaa ataaggattc caataataga tataaaattt gacatggaaa
      241 cgtctataac attaaaagaa taaagtgttg aagaaagtaa actgattgaa ataacccctc
      301 cgtaaaaaat agtctgtgaa tatacccact ttttttcaga agacgtactt cgtttggttc
      361 taaaacctat ccctgcattt ctactcttat caaaaaacca aatatttaga agtaataata
      421 ttgctattat taagataaaa ttactgtaaa aatccaaaag ctatcctcct agcaactatt
      481 taaataccca aaaaaattac ctctagataa tatcatacct acgttttcac ttataaattt
      541 tttcataaaa aaagcgttta gaggcataat cgttaataca gatggtaacc aatgtatctg
      601 gtttgaactg ggatatcacc ctctccatat atgtattact aagactaata gatactataa
      661 aagacttgat agttttgcat tgattttgcc taatactgag cataggtgta tgctaaacga
      721 tactataagg agtgtgtaca taatgaaaag aattctgttt atcagtttat caaatttatt
      781 gttacttact gcttgtggga cttctgcatc aaagaacata ccttcagata cgcaaatttc
      841 gaatagtatc gattcaacaa tttctacaaa tgagaaaaat tctccggtag aaacttccga
      901 tataaaagtt tctacctctg caaaaaacag ggtaaagaaa ctgtcagcac gtctccttta
      961 tttcaaacaa actctgaatt agcttcttca tcttcttcag caaataatga tgatagtttc
     1021 gcgtttactc ctaactaaat gcaaccttta caaatgcttt acgataacaa gcctgagcta
     1081 aatgatcctg atatcagttt tgcatttttt gaaatgattg gatcggatga tctttttaaa
     1141 actactagtt tgtctatccg aaaataggac agttctggca gtataggttt ttatcgagtt
     1201 tctccaccag gcctcgttac agagacagat tctctaggcg aaccttttga ataaatcaaa
     1261 aatcatgaac aatcactgcc aactaaataa agtgccttca aaagcttggt cttctcgctt
     1321 ttgaaggcac tttatttaaa tctttatgaa actaaagtat gttcatattt gttgctatag
     1381 ttatatagat attctgttgt ttccaactca taaacgtgag tatattttgc cactactttt
     1441 ccgcattttt ctaatatttc attgcgatcg acgtcatgat attcgtctac gcagatcaca
     1501 acacaatttt ctaatttact gattacttct ccaatcactt tttgtttcaa gccaataggt
     1561 tggcaaatat atttttttcc taaaatgata gtttcgttat tcatatttca tccatcctta
     1621 atgttttttc attatttaaa atgataataa cattaaaata tgattatgaa aatcaataac
     1681 acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag aaatgcccac tgtctttccg
     1741 gctcttttta ttcttgattt ctcttctttt ttgtttcaat agacgatttt agttattaat
     1801 aaatgaaaaa gcatttatat taaaatttgc taataaaaga tctacatatt tttctagaaa
     1861 tgcttgatcg atttcatcat accctttcgt tactccgcaa tcgatatcta aaacaccgat
     1921 taaattacct tcttttacca ttggcactac gatttctgat tgtgcggctg aatcgcagga
     1981 aatgtaattt gcatgggttt tgacatcatc aacgatcaat gtttcttgtt tttcagctga
     2041 ttctccacaa actccttttc ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg
     2101 tcctaaaatc aattcattac catcgaataa ataatatcct gcaaagacag tctcaggcaa
     2161 agtctcattc aacaatgcag atgaatttgc taaatttgct aaccagttat cttctgcttc
     2221 taataatcct tgttgctgta ataataaaat ttcgtatgca tcttttttct gtttttctgt
     2281 ccacatgata aaaaccgctc ctttttgatc tatggagaag attatagctc agtttttttg
     2341 taaacgctag gttttctcct caaaaaaacg agatacgtca ggttaatcac cttgaacgca
     2401 tctcgctgtc tttttctatt ctgcttgttg gatttctgct gctaaatcag cataatcaat
     2461 cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg
     2521 gctaatctca acttttcctt cagccacttc aaaatccatt acatgtccgc caaatgtatg
     2581 tgtatcatcg ataaaatgca ggtgaaatcc cgctgcagca actccttgga ataattcagg
     2641 cgtaaagaac ccgactaatg ttccttggac attgctttgt tggaattctg gttgagactc
     2701 tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat
     2761 atttttgaat aatccagtta tcttcactgc caaaaatagg ttttccccgg cttctcttac
     2821 taatatgtct tttttcaatt gttctccatc tgcagttttt gagacagaaa aactagtatc
     2881 tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca cttccatcta attgggcaac
     2941 tgagccatct gatcttcctt gataagctac gccatctaaa aagatgactt caccatcaag
     3001 cccatgcaat gttcccaatc ccagagttcc tttttctaat atatgagcaa tcgtttctgt
     3061 tccatccatc aagccagcca tcaaagctcc taatgtcccg tgctggtata agattttttc
     3121 agtcatgtcg acacttcctt aatagaattg atctggtaag attgtttctc ccaatttctc
     3181 attatcacgg taatcaatcg gaatatcaat aattacgggt ccttctgtcg caaatccttc
     3241 ttctaaagct ttttctaatt cctctacact tgtagctcgg attcctttag cgccaaaagc
     3301 ttctgcatat ttcacaaaat caactggacc aaagtctaca cctgacgccc gctgatattt
     3361 cattttttct tggaactcta ccatattgta gtgtccatca ttccaaatca aatgaacgat
     3421 attcaatttt ttacgtaccg ccgtttctaa atcctgggca gagaataaaa atcccccatc
     3481 gccggaaact gatacgattt gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg
     3541 caatgcaaca ccaagcgttt gcataccatt actgaataac aaatgacgag gttcataact
     3601 tctaaagtgg cgtgccatcc aaatataatg actacctaca tctacggtaa cagtcatatc
     3661 atctgttact tttgactgaa gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc
     3721 tccttttgat tgaacaatgt cccgctccgt taattctgct tgaagagatg ctaaatattc
     3781 ttttgcatct tctgacactt gttgaggctc taatgaacct gtcaataaat caagagtggc
     3841 tgagatatca ccgatcaatt ctctttcagg ctgcataaaa ggatcgattt cagctggcgc
     3901 ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc cagtttcttg cttcatattc
     3961 gattggatca tagccaatag caataactag atcacttcgt ttcaaaagca tatctcctgg
     4021 ttggtttcta aataaaccca cacgaccaaa gaaatgcgct tctaattcac gtgagatcac
     4081 tccagctgct tggaatgtct ctacaacagg caatgctgtc tttccaacta attgcctgat
     4141 ggctaacgtt tctttttcac ttgatcctcg catacctact aatagtaccg gtagttttgc
     4201 ttctctaatg cgttcagcta ggtaacggat atcatctgca gaagcagaac ctaatttagg
     4261 atcgctcaat ggcttgatga cattcccttg gacaggtgca tccactacat cttgcgggat
     4321 actgataaag ctcgcgcctt tttttgaact cttagccata cgataagcat tggcaatcac
     4381 ttctgaaatt gtttctggat cttggatttc tgcactgtat tttgtgattg gctggaacag
     4441 tgctgcatta tccatgcttt gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc
     4501 ggcaattgct aaaactgggt caccttctgc tgtcgctgtt accaaacctg tagctaagtt
     4561 actagcacca ggaccacttg ttgcgataac tactcctggc tctcctgtaa tacgtccgat
     4621 tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc acgattaatt caggaccttg
     4681 atcctctaat tcattaaata caccatcgat ttttgcacct ggtataccga atacataatc
     4741 aacatgatga ttaatcaaac tttctactac agatttcgaa ccttgtttca ttttttcgtc
     4801 cattgaacat cgtccttttt atgctttatt tttaattatt agttagaaat aatcacaaat
     4861 aattgataca gtttctaaac ctgtttcatt caaaagtata gcacgtttta tttcatttgt
     4921 ttagccatat gtttcaaaaa atgaaaaaga atgacagttt ttacataaaa actgtcattc
     4981 ttaattgaaa gtttcaattt ataaagtcta cagatccata gttctcatca atttacgatt
     5041 gttgaagtaa atcaaaccaa atacgataga aggacctagt agaatacacc attggggctt
     5101 tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa tctagtaaac tcacatacca
     5161 ttttttcaac gtatagtaga acgctatccg tagaacttga tttggtttcg acgctggatt
     5221 tctcacctga aaataccagt agttgatgct aaaggaaact gataaacaag caagtaaaac
     5281 aaacaacgga atcaaccatt ttccataaga aaaacgtata aaaaagaaac aatcaaaaat
     5341 cattatcaca ctaactatcc aagcaaatag ccaataaaaa aatcccttta gccaaaattg
     5401 gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt ttttcttttg ctaattcttc
     5461 tgtcatacct atcatcacga tgcaacctgg accaataaaa agtaaagaaa aaataaagga
     5521 aaagacattc cttccatcta tcgacaaaag gaacgcagct gctatataca agatattgac
     5581 acaccaaaaa caaaggctac ttgccaatga ttgatacaac caataaaaga attccatata
     5641 aacatttttg tcaaaacgct gctctttcat aatcacctcc tattccttca ttcctgatga
     5701 agcgatccct tctacaaaat atttttgcat cgctaaaaat ataaaagcaa caggaagaac
     5761 agaaacaact gaggcagcca tgattaatgc gtactctgca tcaaatagcc caataaacat
     5821 tttcaaccct aactggattg ttttattttc cgtagaagac aaataaatga atggccccat
     5881 ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt
     5941 aagaggtaaa atgatcttgc gatagattcc ccactctgat aagccatcga tccgcgcact
     6001 ctcacataaa gaatctggga tactactgta gtattgcttc aacaagaaca caccaaaagc
     6061 attgaacgct tgtaatagca ccaacgacca caacgtatat gttaaaccca cttttctcat
     6121 cataatgaac tgcgggatca tataagactg ccaagggaca gcaatcgtgc cgatatatgc
     6181 taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa aacccgtaag cagcaaaact
     6241 agaagtaaac aactgaatca acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa
     6301 tgtcactaaa ggaatacgag accaaataac cagataattc tctaaatgcc aagttttagg
     6361 gatccattga atcggaatcg tgaacacttc attatttgct ttgaatgaag cagaaaccat
     6421 ccataaaaaa ggaatgatcg tcaataaagc taatacaatc agcagaatca tacccaacca
     6481 ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt tttctttcat ttcgaacacc
     6541 tccaaagtta taatcttcat ttctttgtaa tgattacaat ccagaacaac ggttattata
     6601 gctgattcat ttccgttctt cttatgtttg taagcgctta tttaacaagt tatgtaaatc
     6661 tctttatttt ttttgattta aaaatgaaaa aatacaaaag caagactgtc atacgtctcg
     6721 cttttgtatt gaaatttctt cgattagaga tacttattac tgttcaaaag cttctttgaa
     6781 acggtgaatc aattcattga ctacaggtga taaaatccga ttctttttcc agactagcat
     6841 gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc actcgctttg aaagatgact
     6901 tacagcacct tctatgacta aagcagcacc tacctgattc tcaattagcg caaatacatt
     6961 gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc ttttttatgc ctaaccagtc
     7021 agcaaacatt cgctgtattt ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc
     7081 ttcttttgtt atcacgtctt tttttgctaa aacagaattt tctaaaatga gcaatcccca
     7141 tttttccttt cttggtaaca aaatcgtttc gtatttctct gtatctactg gatctagcaa
     7201 aatcgctaaa tctaacaggc ttttttctag tcactccgtg atatcatcac ttgttccgct
     7261 gtggatatga aattgaacgc tgggataatc acggatcaac ttttctagta atagtgccaa
     7321 tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat cggccgctga ataattgttt
     7381 ttttctgttc tcgaattctt gagtggtttg attcgttaaa tctaaaattt cttctgctcg
     7441 gcttttttag aaaataacct gcttctgtca agatcatttt gcggttttcg cgtacaaaca
     7501 gttctgttcc tagttcctct tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa
     7561 gatgtagaac ttctgcggct tgagagattg ttccttcttc tgcaattgtc caaaaatatt
     7621 ttagtaagcg taactccata gcgatgctcc ttgatttttt tacttgatac attccatttt
     7681 atttctagat aacacacgat catatttctt gatttttcct tgtaaacgtt ccaaacattc
     7741 ttgcatttca gcaatttttt tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg
     7801 ctcaaataca gttgcttctc cttcgctaga taggcgaata tattcaatca aagactcgat
     7861 cgataatccg gcatttcgca tacagatcgt aaagtcgacc cattttaggt cttcttctgt
     7921 ataatcacgg attccgcctt ttgtccgatt aactggtgga atcaacccga ttcgttcata
     7981 atagcgcaat gtatcggccg aaatgccctt ttcttcactg acttgtttga tattcatctg
     8041 atcactcttt ctcttaattt cttgccactt tacttatgta ttttatttat tctacaactt
     8101 taagtgtact ccaagtcaat actcatttct attaaaacta agctggatta tctatatcgg
     8161 ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt tattcttttt tggatatact
     8221 tttaacaatt tagggaaatt ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa
     8281 gttattatta tcgacatcgg cgggaattgc tttagagaat atggatatta tgtttttagc
     8341 tttttcatta tcttcaatga ttacttcttt tcatattagt ggtacgcaag cgggtttcat
     8401 ttcaacgatc actaatttgg ggatgctcat tggaggtatt ttttttggca tcatagctga
     8461 taaatatgga cgtgtgaaag tattttctca aacggtcctt ctgttttcga ttgcttcttt
     8521 attgatgtat tttgcttcaa acatctactt ggtttatctc tttcgcttta tcgcaggtgt
     8581 aggagcggga ggagaatacg gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa
     8641 acaaattgga cgagcttctt cggttgctgg tattggtgcg caggtcggtg cggctttggc
     8701 tgcgatttta gctgctgtga tcattccaac gtttggttgg aagatgttgt atgtcgtagg
     8761 tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt
     8821 caaatctgta caatctgccg aaaagaaaac caaacttagc agactgtttg aatcaaaaca
     8881 tcttgcttgg caaacatgtg gccttagtat catggtaact gtccaaattg ccggctattt
     8941 tgggttaatg aattggctgc cttcaattat gcaaaatcga ttagggcttt cggtttctgg
     9001 gtcttcattg tggatggtca gtactatttt aggcatgtct ttggggatga tgacttttgg
     9061 cataatcatg gataaattgg ggcctaaact agccttttct ttgtttttaa tttgttcatc
     9121 cttatctgtc tatctacttg tgttagccca tagccagtgg agtttggtag cagccgctgt
     9181 gatagtcggc tactttatca atggcatgta tggtggatat ggcgcagtga ttagtagttt
     9241 gtatccaaca gaaatccgcg cgacggcgaa taatttcatt atgaaccttg gacgagctgt
     9301 tggtggtttt tcttctatcg tcatcggttt cttgatggat cattatagtt taactgccgt
     9361 aatcttgttt ttgagcggta tttacctagt cagtctgctg gtcttgacaa ctcttactgg
     9421 tgttacagca ttaagggaaa gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa
     9481 gaggtgtggc aaaagcttgc atctgcggta ataagaccag ccaaatcaaa aacatgagac
     9541 actgtttccg tttggctggt cgtattactt gatgcagggc agctttttca actgctaatt
     9601 tccttctatc tggcggctac tattctttga cgtccgtcat ttcaaagtac tttactaata
     9661 acttagcata acgaaaaact gtggatacac tcgtatattg agcgtctgca taatcttcca
     9721 aatttttctc aggatgttga tacatgaatt caatcaaatg ataattatat gattttctgt
     9781 agaaatgaag ataaataatg tagattggga agttctcctg cttttccata gaaaagcgcc
     9841 gattattctc attcttcaaa atgaccttat caggatattt ttggaacaat tcttctaaat
     9901 ctgttagata attcaagata gacttacttg ataccttcaa ttcgttttgt agttcttcta
     9961 ttgaacacca acgttctgtt tcaagcagta aatgaatcat tttaattttc ttttgagaaa
    10021 cttttgttaa aaaagtctgc atattttctc ctcctgtatc tactttacta tactacatac
    10081 aagaaaaaaa acggatacaa atatcatatt gtcacaccgt caatagtgca taaacctgtt
    10141 attatttctc tttttttcct aagtattcta ttgatatgaa tcatttaatc gtttattata
    10201 taaatgtcaa taatctaata ataaaataac gtacatattt tattattgca tacttaataa
    10261 cagaactata gcaaaaaatc ccgtgtatca aaacgggatt agattaagtt tcctcaatca
    10321 ctattcataa cacaattatt ataacaagta agtaaacaat tgaaaaggac atttattgaa
    10381 aacggttgtt ttttacttaa ggaggaaatt tagtgaaaaa attagttgca ttgtttttgg
    10441 tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc tactactcaa agcacaagtt
    10501 cagctcaggc ttccagcagc agtaccacga tctccgaaag tacggcagat tcatcgaaag
    10561 aacaagaaca aattgaaaag aaaaaagaag aagagaaaca aaaagcggaa aaagctgcac
    10621 tagaaaaaaa agtgacagaa gcggaatctg ctttgtcaca agctgaagca aatcctacaa
    10681 aagaaaacta tgcaactgct tcagcagcta tccaagcgct acctgaaaac aaacaagagt
    10741 tgacaagccg tcttgctgca attgattcca caatcaaagc aaaagaagca gaagaagaac
    10801 ggaaaaagca agaagaaatt gcacgacaac aagcagaaca agcgcaacaa gaagcaatcg
    10861 cacagcaaca agcagaacag gcgcgacagc aagcagcagc tgaacaggcc tctcagcagg
    10921 cacaagccgc agctcctgag caaaataatg aacaaactgt ctatgtcaca ccgaccggtt
    10981 caaaatacca tacacataaa tgtggaaatg gtacgtatac ccctgccact ttaagcgaag
    11041 ctcaagctcg tggtttaacc ccttgctcca aatgttatgg aaactaattc aaaagttata
    11101 ctataataat aaaattcttc tgaaacataa aaaaatagaa gctggcgttc cagatagtaa
    11161 accaaaaata gcagtttaca cgtccatttc gtcagcttct ctcttttttc atttagtctg
    11221 ttccctctgc ttttttggac atcttcattc attctttctc aatcaaatag tacacctctt
    11281 tttcttttcg cagtttatat cttccttttt tcaatttcat tgaaaactgt ttcgaatgtt
    11341 tatgttttac atggatttta agcaggagct cctcatgttg caagcgatag ttcctgcctt
    11401 ttaaatcaga aaatctcact agtccgtatt catcagaagc tttcacaaat ggaacttttt
    11461 ctctagtaat atagtgtttg ccgttgagcg tttttacaga aaagaccatt ccttcgattg
    11521 gtcgtccgtt tgtgttcttt acataaaaag ataaaggata gtttcgctta gcgatggcca
    11581 aagaatctaa ataagcgatg agtagccaaa ctaagccgat tgatccaagt agtataccga
    11641 taaaccaaat aaaaaatttc cattttttcc attgatcaat tccttggatc gctttttttg
    11701 cctcttttgc ttgataagga atacgatgcc ccgtcacaag cagtcgatgt gtattgacca
    11761 tataaggagt acaggtaagc aaagtcacga gatctctgcc tttttggata cccaaagaat
    11821 ctacttcatc aggtaagacc acttggattt tttctacttg atacgccaat gtctttccat
    11881 tgatctggat gaaaaactgg tctccttttt tcaacttagg caaatctgta aataattttg
    11941 cttctgggag tccacgatgc ccagaaagaa cggcatgggt gtttttcccg ccagtcggat
    12001 aagaactttc ttccaataat gacgttcctt tttgcaaaaa tatttccgtt gtctgatcga
    12061 atataggtaa gctgacattg atttttggga tttcgatgac tccaagcata tgttccataa
    12121 aaaaggcatt actaggtaaa tcttttaatg cttttgcatc aactgcttgg ttgaaactgc
    12181 ctatccctgg tgatacgttt tgctctgcca actgttggtt tttctttgtc attttttcct
    12241 gctgtttttt tatttcctct gaattcttat tcgatgcttg cttttgataa tggatgatca
    12301 tttgctgggc aagaaaatca ttcagtgaat cttctacaaa aggataagaa aagatcccga
    12361 tcccaacgac taaaatgatt gccatgaaga tgtccagtat tcttattctg cttttattca
    12421 tcatgtccac ctattttcta atatgtatat gaagaggt
//
LOCUS       DAZUMT010000085        12181 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000085 DAZUMT010000000
VERSION     DAZUMT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.086"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..620)
                     /gene="frr"
                     /locus_tag="ACG8FM_002155"
     CDS             complement(63..620)
                     /gene="frr"
                     /locus_tag="ACG8FM_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS5094802.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(623..1345)
                     /gene="pyrH"
                     /locus_tag="ACG8FM_002156"
     CDS             complement(623..1345)
                     /gene="pyrH"
                     /locus_tag="ACG8FM_002156"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS5094803.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1481..2362)
                     /gene="tsf"
                     /locus_tag="ACG8FM_002157"
     CDS             complement(1481..2362)
                     /gene="tsf"
                     /locus_tag="ACG8FM_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS5094804.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2461..3243)
                     /gene="rpsB"
                     /locus_tag="ACG8FM_002158"
     CDS             complement(2461..3243)
                     /gene="rpsB"
                     /locus_tag="ACG8FM_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS5094805.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3602..4081
                     /locus_tag="ACG8FM_002159"
     CDS             3602..4081
                     /locus_tag="ACG8FM_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5094806.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4298..5389
                     /gene="phnW"
                     /locus_tag="ACG8FM_002160"
     CDS             4298..5389
                     /gene="phnW"
                     /locus_tag="ACG8FM_002160"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS5094807.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5382..5510
                     /locus_tag="ACG8FM_002161"
     CDS             5382..5510
                     /locus_tag="ACG8FM_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS5094808.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5514..6059
                     /locus_tag="ACG8FM_002162"
     CDS             5514..6059
                     /locus_tag="ACG8FM_002162"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS5094809.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6513..8204)
                     /gene="argS"
                     /locus_tag="ACG8FM_002163"
     CDS             complement(6513..8204)
                     /gene="argS"
                     /locus_tag="ACG8FM_002163"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS5094810.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8624..9571)
                     /gene="arcC"
                     /locus_tag="ACG8FM_002164"
     CDS             complement(8624..9571)
                     /gene="arcC"
                     /locus_tag="ACG8FM_002164"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS5094811.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9686..10705)
                     /gene="argF"
                     /locus_tag="ACG8FM_002165"
     CDS             complement(9686..10705)
                     /gene="argF"
                     /locus_tag="ACG8FM_002165"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS5094812.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10796..12025)
                     /gene="arcA"
                     /locus_tag="ACG8FM_002166"
     CDS             complement(10796..12025)
                     /gene="arcA"
                     /locus_tag="ACG8FM_002166"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS5094813.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3335 a         2528 c         2203 g         4115 t
ORIGIN      
        1 aaagaaatat aaataaaaac agaggaagcg gaatgatccg cccctctgtt tttatgattt
       61 tattaaactt ccaataattc tttttcttta tcagcagcga tcttatctac ttctttgatg
      121 ctgttatctg tcaatgtttg tacgtctttt tctaatccgc gcaaatcatc ttcggtaata
      181 tcaccatttt tttgttgttt tttatattca tcgatcgcat cacgacggat gttacgaatc
      241 gcgatttttg cattttctgc ttctttttta acatctttag ctaattcttt tctgcgttct
      301 tctgtcagtt gcgggataac taagcgaatg acatttccat cattggttgg actgattccg
      361 atatcacttg ccataatagc tttttcaaca tcttgaagaa tgctcttgtc aaaaggtgtg
      421 atcattaaaa cacgtgcttc tggaatttgg attgatgcca tttggttcaa tggtgttggc
      481 gcaccgtagt agttcacagt aatacggtcc aaaaggctag cgttcgcacg gcctgcgcgg
      541 atttgcccaa gttcacgttg aagattttgt gctgcttttt tcattttttc ttttgcttct
      601 gtcataattg catctgccat ttttatttcc cccttacagt tgttccgata ttttcgccta
      661 agattgcacg gcgaatattt ccgtgttcat ttaaattgaa gacaagcagt ggaatatcat
      721 tatccatgct tagtgaactt gcagttgaat ccatgacttg taatcctttg gcaataactt
      781 ccagatgagt cagttcttca aattttactg catttgcatc tactttcgga tcagcagaat
      841 aaacaccgtc tacattgttt ttagccatca ggataacatc tgcgccgatt tctgccgcgc
      901 gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc ggtaccgcct gcaaagataa
      961 cgatccgtcc tttttctaaa tgacgttctg ctttacggcg aatatatggc tctgcgattt
     1021 gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc cacgttttcc aacgtatcct
     1081 gtaaagctaa ggcattcata actgtagcta acatacccat gtaatcagct tgagcacgtt
     1141 ccatgcccat ttgtgctccg atttgtccgc gccagatatt tccgccgccc acaacgattg
     1201 ccatttcaac gcccagctcg tgaacttctt tgatttcttg gatgatttct ttgattactg
     1261 gtggtttgat tccaaatcct gcgtccccag ctaaagcttc tccactcaat tttaagacta
     1321 cacgctgata tttcggtgtg accatgttca ttcctccaat attttctatt gccattttac
     1381 catacttaga tgatttcgac actctaagac tggtttttct tgttttgctt aaaaaaaggg
     1441 agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa ttattttttc acttggctca
     1501 taacttcttc tacaaagtta tcttcgcgtt tttcgatacc ttcgccaact tcgaaacgta
     1561 cgaatgtttt aacagttgcg ccttttgatg caacgtattt ttcaacagtc atatcaggat
     1621 ctttaacgaa tggttggtcc accaaagcga tttctgcttt gaatttcttc aagcgtcctt
     1681 ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt caatgcttgt tctgtaagaa
     1741 cagttttttc atgttctaat tcagcttctg gaatttgtga ttcgtttaca taacgaggat
     1801 tgatagctgc tacgtgcata gctacgtctc ttgcaactgt ttcgtcagtt gttccatcta
     1861 gaacagtcaa tacggcaata cgtccgccca tatgcaagta tccaccaaat gcagcattgt
     1921 cgtctttttc tacaacttca aagcgacgga agctgatttt ttctccgata acttgagttg
     1981 cttcgatcaa gtcagattca attgtacctt tatctgtttt gattttcatt gcagcttcca
     2041 tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc ttttactaaa tcttggaaca
     2101 tttcgttttt agaaacaaag tctgtttctg agtttacttc aacgattgct gcagtgttac
     2161 ctttcacagc aactgaagcc aatccttcag ctgcaatgcg gtcatttttc ttcgcagcct
     2221 ttgccattcc tttttcacgt aaaagatcaa ctgctttttc catgtcgcct tctacttcaa
     2281 ctaatgcttt tttcgcatcc atcattccta cgccagtcat ttcgcgtagt tcttttacca
     2341 ttttagctgt aacgtctgcc atgtttaact cctcctgtgt gtttttcttt ttaaaaaaag
     2401 ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga aacagctcca tcattcaaaa
     2461 ttattctgct gaagaattgt cgccttcaac aacatctacg atttcttcga ttgatgttgc
     2521 tgtgttttct tctgtgaaat cttcttcaac tacttgatct tcaccttggt ttccttcgat
     2581 gaaagcatca gccatttttg aagtaatcaa tttaacggca cgaattgcat cgtcgtttga
     2641 agggattact acgtcgattt catctggatc gcagtttgta tcaaccatag ctacgattgg
     2701 gatatttaat ttgtgtgctt cttgaacagc aatacgttct ttacgtgggt caacgatgta
     2761 cattacatct gggattcttg gcatatcagc gataccgcct aagaattttt caagacgttc
     2821 acgttctttg ttcaaaccag caacttcttt tttaggtaga acttcaaaag ttccatcttc
     2881 ttccattgca ttgatttgtt tcaaacggct gatacgtttt tggatagtat cccagtttgt
     2941 caatgttccg cctaaccaac gatggttaac gaagtattgt cctgcacgag ttgcttcttc
     3001 tttgattgct tcttgtgctt gtttttttgt acctacgaat aatgctacgc cgccttcttc
     3061 agcaacgctc ttcatgtaat cataagctgc atctactaat ttaactgttt tttgcaagtc
     3121 aatgatgtag attccgtttc tttctgtgaa gatatatttc ttcatttttg gattccagcg
     3181 acgagtttgg tgaccaaagt gtacgccggc ttctagtaat tgtttcatag aaattactgc
     3241 catgtttgtt tcctccgatt tggttttatt tttcctcttc ttatcttcta ccttgccggc
     3301 aaactttttc aagcacctac cgacaatcca ataagaatgt ggatttgctg atgcataaag
     3361 caccctcagc tattatacag acaaaagatc cgtatcgcaa gagcaaatat acttttctgc
     3421 tcaacattca atagtttacc tgaaagataa aaagaattca agaaaaacag tagatttctc
     3481 tttgtttctt tattatcctt tttccataaa ataattttag gtataaaaga cgaatattat
     3541 tgggcaatcc ttcttttttt tgttatgata catgtgaaat ctttcgtaaa ggagaaagaa
     3601 aatgcgcaaa gctcaaagac atttgctgat taaacaagtc attgaagaat tcccgatccg
     3661 tacgcaagaa gaactattaa acaaacttgg gcattatggc gttaccgcca cgcaggcaac
     3721 gatttctcga gatatccgtg atctaaaaat cgtgaaggca cctgatgaaa atggcgtttc
     3781 ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact aggactgagg aagaggatcg
     3841 gctggttcaa atgattgaag acattgtatt gaaagtcgaa cgagtccact ttttgacgat
     3901 cgtcaatacc ttgccagaca atgctcattt atttgcatct gttttagatg agatcaaacc
     3961 tccctctatt gtcagtacaa ttgctggatt cgatacgatc atggtcatct cggaaaatga
     4021 ggaggacgca aaacgagtag aagaatttct tcaccatcca actgaagtat cactttttta
     4081 atcagagcac gtattcctaa tatggaatgc gtgttttttt attttcaata ttttccttat
     4141 tgaataaacc tttacactct ttacaaaaat acatcatttt ttattcaaat ttttcaacac
     4201 cttgattgaa tcacaataat catcatttat attggatgtt aacaagagca gcttcttgct
     4261 ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg tataaattat taacttctgg
     4321 accattgacg acttctgaaa cagtgaaaaa tgctatgctg atcgatcatt gtacttggga
     4381 tcaagaatac aaagaaatta cacaatggat aagaaaaaaa ttattggatc ttgctcaagt
     4441 atcggaagga atatacacaa cggtcttaat gcaaggtagc agctccttcg gggtagaatc
     4501 ggtattaagt tctgtgatca ggccagaaga tactgtactg atttgttcaa acggcgcata
     4561 tagacaacga atgatcaaca tgtgcgaatg tctgaaactg aactataccg tttatgaggt
     4621 atcggaacat caaatcctcc aagctcatgt gattacacag ctgattgaag agaatccgtc
     4681 tatctccgca ctagcaatgg tccatagcga gactacttcc ggtattttga ataatttgga
     4741 agagattgct gctgtcacac aagaaagaga tcttttgttt atagtggacg cgatgtcaag
     4801 ctttggtggg ataccgattt gtgtagccga actagggatt gattttttga tttccagctc
     4861 aaacaaatgt attcaaggtg ttccaggctt ttcttttgta cttgctaata aagcggaact
     4921 ggaaaaacga aaaaatgtta gccgaagttt gtcacttgat ctatatgacc agtaccgcgt
     4981 gatggaaaac gagaatggca aatggcgttt cacttcccct acccatacgg ttttggcttt
     5041 tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc gaaactcgat attcacgtta
     5101 tctcgccaat aatcatgtgc tacgacaaaa aatgacagag atggggttcc agcctttcat
     5161 tcaagaatat caggggccat tcatcacctc ttttctttac cctaaggact ggcgatttga
     5221 ttttaatcat ttttatcagt atttaaagca aaatggattt gtgatctatc ccgggaaaat
     5281 cagcgatcat gaatgttttc gtatcggaaa tattggcgag atctatccgg aagatatttt
     5341 ggaactgaca gcaattatca aagactataa ggagaacgtt aatgattaaa actgtaattt
     5401 ttgattgggc aggaacaact gtggactttg gctgtatggc tcctgtccac gcttttagaa
     5461 atgcttttct agaaaaagga atccaactga ctgacaaaga aatccgctaa ccgatgggaa
     5521 aattaaaatg ggatcatatc caacagcttc tttccctccc ttctgtcaaa aaacaatgga
     5581 tagctattta tggacaacta cctcaggaaa aagaggtgga ggaattgtat caacaatttg
     5641 aacattatct gtttaaagat ttggtcaagc attctacctt gaaacccgac acattggaaa
     5701 cgatcgaacg gttgagaaac gctgggatca atattggttc cacgaatgga tttaatgctc
     5761 ctatgatgac aatcgtagca gaaaccgcaa aaaaagaaag gtattcacca gacttcatgg
     5821 caacttttga agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc cataaaaaca
     5881 tgcagtactt caaaaacaag tcggttgatg aagtaatcaa agtcggagat acggtctcag
     5941 atatccagga agggaaaaat gccggagtgt tgacagtagg tgtaattgaa ggtagttcgc
     6001 taatggggat ttcttatgat gagtaccaaa aattagagat ggggaaaaag atcgtttaaa
     6061 acagtctgtt gctcttagat tccaacaagc cggcgctgat atttgtgtaa acaatttaaa
     6121 agagttaaca gaatacattt tgtacgctga catgaatcca gaatgacttt tatacaaaat
     6181 aagaggttgt gaaaaagctg tcctgtatca agtaataaaa acagccaagc aaaaatagct
     6241 cctcatattt ttcgtttggt taggcttatt accgcagatg caagcttttg aacaccgttt
     6301 attcgaaaaa aaaaagggag ctgtgacacg acttttgtca cagtcccctt atttgcaaac
     6361 aaacagtgtt taagagacaa cctctccact aaacggtgtt cattcagctg actctcggta
     6421 tttgctcaaa aaacgataaa atttgaaaag ctattttctc ttttgtttct ctaatactcg
     6481 gatcagaacg ctgatttcac attctctttt tcttacatct tatctggtgc atgaagtcct
     6541 agcaaacgaa ggtcttcttt caataataca gtcactgcat aaactagagc taagcgtgct
     6601 tctttttgtt catcatcagc taaaatcttc gtatgagcat agtatttgtt gaatgcttgt
     6661 gctaatttga ttgcatgttt cgcaatcact gacggttcgt atttttcccc agcagacaag
     6721 accgtttcag ggtatttttg aactaattta accacttccc agctggtatc gtcattcaat
     6781 gcatagtctg ctgtttctga agggacaaat cctgcttttt ccaacaggct gactgcacga
     6841 gcatgtgtat attgcacata aggtccagtt tctccttcaa atcgaacaac ttcttccagg
     6901 ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac tgccccaacc
     6961 ccaacttgtt tagctacagc atctttgttt tccaaatcag ggtttttctc gctgatttgt
     7021 tctttagcag attggattgc ttcatttaat acttcttcta ataagacgat ctttccttta
     7081 cgagttgata attttttacc accttgcgtg atcaaaccaa atggaatatg gtgcatgtcg
     7141 tcagaccagt caaagcccat ttccttcaag acagctttca actgtttaaa atgatagctt
     7201 tgttcattcc ctactacata tagagattgt ttgaagtcgt aagtacgttt acggtacaat
     7261 gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt aatcaacgct
     7321 ggattcaggt cataagcaga taggtcaacg atctctgcac ctttatcctc gttcagcaaa
     7381 tgtttttctt ctaaaagttt gacgatttca tccatcttgt cattgtagaa agcttcaccg
     7441 ttcaacgaat cgaaacgtac ttccagcaga tcatagattt tattgaattc tttcatcgat
     7501 tcatcacgga accattgcca taattgaata gcctctttat cgccttcttc cagacgtttg
     7561 aaccaagcac gagcttcttc atttaattct ggttctgttt cagctacttc atggaattgg
     7621 acgtataaac gaagcaattc attgattggt tctgctttaa cagcttcttc tgtaccccat
     7681 tttttgtaag cgacgatcag ttttccaaac tgtgttcccc agtcacctaa atggttgatg
     7741 cggattggct gataaccgat tttttccata atgaaaccga tcgaattacc gataactgtc
     7801 gaacgcaaat gtcccattga gattggtttg gcaatattag gagaagacat gtcgattggt
     7861 actgttcctt gattcccgat gttgctatcc ccataatgtt ctttttcttt cactactgtt
     7921 tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa agttcaagta aggtccgaca
     7981 acttcgattt tttcaaagtt ggcagaatcg attttttctg ctaaatctgc agcgatttgc
     8041 tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac atctccgtgt
     8101 tcagctgatt ttggattttc tagtaattgc tcgacttgtt ccagtgttaa atcatctttg
     8161 acagcgtcat agacagcttt tgcgacaaga tctttattat tcatctttat tcctcctaaa
     8221 gtgttccctt gattgttaaa caaaaaaaat ctctagcgac ggcatactta gtatgccatc
     8281 actagagacg agatttcccg cggtaccact ccaattgtcc taaaaaagga ccagctccat
     8341 ctgatatcgg tagacgaccg tctgtttaaa acagaattct ccaagtgcgc ttcgttgtat
     8401 cttcttcatt cggcttccac cacttccgaa ttcgctagcc aagaatgata caagtactct
     8461 cttgttcaac aaatcttagt cttattaaac tttcttgaat tatatcagaa aaaaaaacga
     8521 aaggctagtc gtttcataaa aaaacaatcc agatggccta aaggagaaga aataccacct
     8581 ggatagctat aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa
     8641 tcgttccttc ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct
     8701 tagcgtttgg ttttgcttca acaaatgcaa tagctgcttc tactttcgga agcatgcttc
     8761 ctggagcgaa ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta
     8821 atttcttttg atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga
     8881 tcagcaaatc cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta
     8941 cggcttctcg tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa
     9001 cagagactgt gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg
     9061 agatgggttt aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg
     9121 tcacagtgga gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga
     9181 ctggtttact tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta
     9241 aagaaaccac atctttatcg atcccttttt ccttcagtac ttcgcccatg gcgttttgta
     9301 accagtaacc gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg
     9361 ttttctcaga atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc
     9421 cgtgggagat gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt
     9481 ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt agacaagatg gcattgcccc
     9541 ctaaagcgac aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga
     9601 attttctaga taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt
     9661 tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg
     9721 ctgccatgat cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat
     9781 atttgctgcg gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca
     9841 tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca
     9901 aacgatctgt gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca
     9961 atttgatacg ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa
    10021 ggacgtctgc tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg
    10081 atttttcagc aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg
    10141 gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca
    10201 tattgtttcg accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt
    10261 cttggatagt taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat
    10321 tccaaactgg aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac
    10381 ggaattcgat tccgtcaaac atacgtccta gtactttagc tgtatcttct gtggattctt
    10441 ttttacctaa ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg
    10501 ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat
    10561 tttttccttc tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg
    10621 aaaaatcgat caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc
    10681 ttccttggaa tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag
    10741 attaaagagg aaatcccgac ggcagctgac agtaagactg ccgtcaggaa acaaattact
    10801 tcaagtcttc acggacaagt ggttggctca tgcagcgtgg accaccacga cctcgtgaca
    10861 attcacttga attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata
    10921 cataatttcg gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat
    10981 cattccattg ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa
    11041 gatcatctaa tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct
    11101 tgatatcatc accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa
    11161 ttgctggatg gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca
    11221 tcattgcacg gttgttcgga attttgattg ccaacacttt ttcaaatcct gaattttttg
    11281 cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa
    11341 cagctactac tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt
    11401 cacgatccaa ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat
    11461 attctgtaaa catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc
    11521 catttcccat tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt
    11581 aaaatggata gtcatcgtct gctgacagat catatagact gctagaacgt acttcgatat
    11641 cttttgtacg aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa
    11701 ggaactcatg aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt
    11761 tattcaagaa ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca
    11821 agtaaagagt ctcgacgcct tcattttgca atgtcttcgc aaagttgtca tgttcttctt
    11881 gagcaatcgg aagatatgga atatcatcga acaacagacg atccataata tctggtgtca
    11941 aattttccac ttcttgtcca ggccgtttca gtaaaactgt ctttaatttc cctatttcag
    12001 agaaaacgtg aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata
    12061 ggatatcctc cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct
    12121 cacattaata ttcgataatt aaataattaa acgtttttct atttttaagc gtaatttatt
    12181 c
//
LOCUS       DAZUMT010000086        12144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000086 DAZUMT010000000
VERSION     DAZUMT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.087"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACG8FM_002167"
     CDS             <1..566
                     /locus_tag="ACG8FM_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS5094814.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /gene="fic"
                     /locus_tag="ACG8FM_002168"
     CDS             967..1572
                     /gene="fic"
                     /locus_tag="ACG8FM_002168"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS5094815.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="ACG8FM_002169"
     CDS             1588..2160
                     /locus_tag="ACG8FM_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5094816.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="ACG8FM_002170"
     CDS             complement(2412..2669)
                     /locus_tag="ACG8FM_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094817.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="ACG8FM_002171"
     CDS             complement(2795..3610)
                     /locus_tag="ACG8FM_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094818.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="ACG8FM_002172"
     CDS             4127..5452
                     /locus_tag="ACG8FM_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5094819.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="ACG8FM_002173"
     CDS             5445..5795
                     /locus_tag="ACG8FM_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094820.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5777..5962
                     /locus_tag="ACG8FM_002174"
     CDS             5777..5962
                     /locus_tag="ACG8FM_002174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094821.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACG8FM_002175"
     CDS             complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACG8FM_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS5094822.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="ACG8FM_002176"
     CDS             complement(7113..8066)
                     /locus_tag="ACG8FM_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5094823.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="ACG8FM_002177"
     CDS             8678..10171
                     /locus_tag="ACG8FM_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS5094824.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="ACG8FM_002178"
     CDS             10306..10602
                     /locus_tag="ACG8FM_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS5094825.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="ACG8FM_002179"
     CDS             10703..11554
                     /locus_tag="ACG8FM_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094826.1"
                     /translation="MKSDDWIEHNWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="ACG8FM_002180"
     CDS             11575..11787
                     /locus_tag="ACG8FM_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094827.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4172 a         1863 c         2268 g         3841 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca actggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaa
//
LOCUS       DAZUMT010000087        12077 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000087 DAZUMT010000000
VERSION     DAZUMT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.088"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..718)
                     /locus_tag="ACG8FM_002181"
     CDS             complement(131..718)
                     /locus_tag="ACG8FM_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094828.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            893..2215
                     /locus_tag="ACG8FM_002182"
     CDS             893..2215
                     /locus_tag="ACG8FM_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5094829.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2370..3146
                     /locus_tag="ACG8FM_002183"
     CDS             2370..3146
                     /locus_tag="ACG8FM_002183"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5094830.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3139..3708
                     /gene="rnmV"
                     /locus_tag="ACG8FM_002184"
     CDS             3139..3708
                     /gene="rnmV"
                     /locus_tag="ACG8FM_002184"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS5094831.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3701..4585
                     /gene="rsmA"
                     /locus_tag="ACG8FM_002185"
     CDS             3701..4585
                     /gene="rsmA"
                     /locus_tag="ACG8FM_002185"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS5094832.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5036..5665
                     /locus_tag="ACG8FM_002186"
     CDS             5036..5665
                     /locus_tag="ACG8FM_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094833.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5789..6898)
                     /locus_tag="ACG8FM_002187"
     CDS             complement(5789..6898)
                     /locus_tag="ACG8FM_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094834.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7107..7529
                     /gene="mgsA"
                     /locus_tag="ACG8FM_002188"
     CDS             7107..7529
                     /gene="mgsA"
                     /locus_tag="ACG8FM_002188"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS5094835.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      7716..7814
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            7852..8388
                     /locus_tag="ACG8FM_002189"
     CDS             7852..8388
                     /locus_tag="ACG8FM_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5094836.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8428..10191)
                     /locus_tag="ACG8FM_002190"
     CDS             complement(8428..10191)
                     /locus_tag="ACG8FM_002190"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094837.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10194..11909)
                     /locus_tag="ACG8FM_002191"
     CDS             complement(10194..11909)
                     /locus_tag="ACG8FM_002191"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094838.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3772 a         2224 c         2459 g         3622 t
ORIGIN      
        1 ttaattataa gttctatcac tttatgttca attagtttgt gctcttatat gtgtgattga
       61 aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc tttattttgt
      121 aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa cggcttggta
      181 agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc gtacgtccga
      241 taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac cctaaaagca
      301 aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt gccatcactc
      361 caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc tttgtaaatg
      421 ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc ggaaaaagcc
      481 caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg atcaatgcaa
      541 cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca acagtcatta
      601 atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct ttacgggaaa
      661 aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta aaatccataa
      721 actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa agaaatacat
      781 ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa attaaaatat
      841 tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg gaatgaaagg
      901 caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg gaaccgtgat
      961 cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt ctacaagttt
     1021 gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat tgacagtggc
     1081 tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg aagctgcgta
     1141 tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact ttcctgctaa
     1201 tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac aattatctac
     1261 cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt tgaatttgtt
     1321 agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat taataccaat
     1381 tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc agttatttcc
     1441 aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt tgttagcaac
     1501 gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa tgaaacggcc
     1561 ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag tcgtttatct
     1621 attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg gtaatttgaa
     1681 tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc tggtcactat
     1741 tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg ggatccgcgt
     1801 gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga caaagctttc
     1861 aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag cttgtattat
     1921 gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga tgtggctatt
     1981 cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg aattgccacg
     2041 tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg gtgctatttt
     2101 cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta taggtgttac
     2161 cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag agtaaaatat
     2221 ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc tttttctatt
     2281 tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt gcttatgtgc
     2341 taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc catttaaatg
     2401 ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa atgggggtaa
     2461 cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg caattaagcc
     2521 atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct ggcagttata
     2581 cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt gtagcattag
     2641 gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa gataaagtat
     2701 ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt catactagag
     2761 aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat atcggtggaa
     2821 tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac ttagggatgc
     2881 atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa gaggctgcag
     2941 caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg gcaccagttc
     3001 cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa aagattgctg
     3061 aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat gcgcaccgat
     3121 tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt cgttgaagga
     3181 aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga aacaataggg
     3241 tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac tcgtggagtg
     3301 atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat tatggaagct
     3361 gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa gaaaaaggga
     3421 agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa aaaggtcgtg
     3481 acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct tttagactat
     3541 ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga cgagttgcgc
     3601 atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg tattacgccg
     3661 aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat atcgtgaaat
     3721 tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat ttaaaaaaag
     3781 tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag aaactgcgca
     3841 aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat tgacggaaca
     3901 attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt tgatccctgt
     3961 attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag atattttaaa
     4021 gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt taaaagtcgt
     4081 agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg aatcagattt
     4141 ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta tctctgctga
     4201 accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata tggaagccag
     4261 tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag attcagcgat
     4321 tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa aagcattttt
     4381 ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata acttgcagca
     4441 ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg aaacagctgg
     4501 aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg cattaagcaa
     4561 tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt gaaaaagctg
     4621 tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt ttcgtttgtt
     4681 tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg ttgtgggcct
     4741 gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat gtttttgagt
     4801 tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac aaggagatgt
     4861 gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc ttatacttga
     4921 tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag ttattagtaa
     4981 gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag aaaaaatgag
     5041 aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg gggtcattat
     5101 cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg cgtctcatgt
     5161 gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg cgctaggtac
     5221 gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat tgtcacatct
     5281 tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg ttttaaaaaa
     5341 tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga tcggtgtgat
     5401 tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta atatgcctga
     5461 aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga ttggtgggct
     5521 tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac tcagcaaata
     5581 ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg
     5641 gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc tcatctagta
     5701 atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg caccaacatc
     5761 ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt tctgtttctt
     5821 tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg cctggtttat
     5881 ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt gtgtaaagca
     5941 tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg gcttcaggtg
     6001 aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg aagataactt
     6061 cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga atacgaagct
     6121 taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag aagttcatgg
     6181 caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa acttctttcg
     6241 gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca gccatagtca
     6301 ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt
     6361 tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat aatggctcat
     6421 ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt
     6481 gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct aagatctctg
     6541 ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat ttcaagccaa
     6601 aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg aaaaccatgg
     6661 caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata tttagttctc
     6721 cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg
     6781 aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa tcagttacag
     6841 aataattttc tgcattgtca tattttttat atacgttttt cagagccatt tctaccataa
     6901 tacgttccac actttctttt ttagttttat taactattta aagtataaat gaaagcgtta
     6961 actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat gtgctagttt
     7021 taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa ataagtatgt
     7081 taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat gaccgaaaaa
     7141 aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa catgaacttt
     7201 ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg atccatcggt
     7261 ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg gaggatcagt
     7321 tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa ccagatgtaa
     7381 cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat atcggaacag
     7441 ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga atcgtccatg
     7501 aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat aacgtttaat
     7561 gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata taatacgaac
     7621 gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat gtgaaaatat
     7681 gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat aaagcgttaa
     7741 gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc ggttttattt
     7801 tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa aatgaagaaa
     7861 caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt gatggtcact
     7921 ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac atttattcca
     7981 caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc agttttagct
     8041 gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt tacattgaat
     8101 gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat cacttggaaa
     8161 aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt gacaccatta
     8221 tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc gccccgcctg
     8281 ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt aggacgtagt
     8341 attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa aacaagcagt
     8401 cttcccatcc cagaagactg cttgttttta ttgtgctgtg aattgtgctt gatagagccg
     8461 tcggtaataa ccatcttttt gaagcagctc atcatgggtg cccatttcga caatagctcc
     8521 ctgatccatc actaatatct ggtccgcatt tttgattgtg gacaggcgat gggcaatcac
     8581 aaaactggtt tttccttcca tcattgctaa gaaggcttgt tggatttttt tctctgtcaa
     8641 tgtatcgact gagctggtag cttcatctaa gatcaacata gggggattac tgatcatcgt
     8701 tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc tttaaccctt gaccgccaat
     8761 cttagtatct aatttttccg gcaaacgttc tacaaattca tacatataag aggcttttag
     8821 ggcatcattg atttctgcgt cacttgcttc cggattccca aaacgaagat tttctcgcaa
     8881 tgtactgtca aatagccaag tatcttgtaa caccattcca aaacttttac gtaatgtatc
     8941 acgttggata gagcggatgt catggtcatc gactgtaatt gttccttttg ttgtgtcata
     9001 aaaacgcatc agcaaattaa caagtgtgga ttttccggcc cctgtttttc ctactatagc
     9061 aatggtttga cctggataag cagtaaaatt gaaatcttcg atcaaacgct gcttggggtc
     9121 ataagaaaaa gaaacatgat caaaagagac ttttcctttg atatttttgg gttccaaggc
     9181 ataggcatct ggcaaatcag gcttttcttc tggctgatcg atcaaatcaa atgctcgttc
     9241 tagaccagaa aaagcggttt gcagttgtgt cgtgattcca gaaagttcga taaatggttt
     9301 agaaaactga cttgagtaga tcgtaaagct ggaaatcaca ccaacagtga ttgacgaacc
     9361 actcttcaat gccaataggc cgccaactaa accaatcgat aaataagcaa ggtgatctac
     9421 aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt gcccgttgtc ctttttcata
     9481 tagatcagca tttagcagtt caaattgctg ttgactagct tcttcttgct gaaaagcttt
     9541 cacgattttc tggttaccga ttcgttcggt cacaaagccc gagatatccc caataatcct
     9601 ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg atccaactca caaggaaaat
     9661 aattggtgta gaacaaagaa cgaccaatgt cagaagtgga cttagacgca tcataaaaat
     9721 aaacgaaaga atgatgattg ccactccaga aaataattga ttgaaggccg cagaaattgc
     9781 aatggaaata ttgtccatat catttgtaaa tcgactgacg atatttccgt gggagttttg
     9841 atcataataa cgaagtggca atcgattcag atgagcaaat gcatcttttc ttaattttgc
     9901 aacggataag taagctaacc gattgcttaa cacttggatg agccattgac tgactacgtt
     9961 gattaaaacg ataccaccga atagaataag taaatggaac agtcgattga agcggacctg
    10021 tccttctcca atcatttgat cgatactttg accaatttgt aacgtcatca caactgttgc
    10081 aatcccattg atgattccta gtatcacagc tagccaaatg tcttttgcat aggcagtaag
    10141 gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt tttactttca taactattct
    10201 tcctcctgtg atgcgacgat ttcctgatat tctgctgatt gagcaagtaa ttcttcatgg
    10261 gtaccctgag cagcaatctt accttgatcc atcactaaga tatgatgtgc ttcttgaata
    10321 gagcggacac gttgggagat aataatcact gtactgccaa gataagtctg tagcgcctta
    10381 cgcaaatcca agtctgtttg ataatccaac gcacttaaag agtcatctaa aatcagtaat
    10441 gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct gtttttgtcc gccagagaaa
    10501 tttttcccat tttccatgac ttttgtatct aaaccatttg gaagctgacg gacaaattct
    10561 tctgcctgag cgattttcag agcttcccaa cattcttttt ctgtggcgtc tttcttaccc
    10621 cattgaaggt tttcacgaat agtcccggta aatagtacag aagtttgggg aacaagagca
    10681 atttgctgac ggagatgatc cagctgccaa gaacgaacat ccattccgtc cattataact
    10741 gtaccggaac tgacatcata gaaacgcgga atcaattgtg tcaaagtcgt tttcccgctt
    10801 ccggttggac caatgattcc aaggactgta tttcgcttca gctgaaatgt aatattttgg
    10861 agagaaagac cagaatcagg agtataacgg aagtcaacat ggtcaaaagc gataattgct
    10921 tcagaagaaa aatcgggagt agtactgacc tcagcttcaa gaatcgaatt ttctgtgtct
    10981 agcacctctt tcacccgatt tcctgaagct tcggcacgtg tgaagatcac gacgaggttt
    11041 gaaacaacga tcagtgccaa aagcatttga ttcatataat tgatcaaagc aagaacttgt
    11101 ccttgttgca agctaccaat atttactttg atcccgccaa ctgtcagcaa acagatgatc
    11161 ccgacattca tgatcaaggt tgtagcaggt gataataggg cagagatatt ggaaacacgt
    11221 aaataattgt tcgctagatc atttgtgctt ttatcgaact tctctgtttc tttttttgtc
    11281 tgcgcaaatg cgcggatgac acgaacaccg ctaagattct gactaacgtg ttcattgagc
    11341 tgatctagtt tttcttgcac tttttggtag agaggaacgg tttttttgat gataaaaaat
    11401 aaaatgattg agaaaatagg caataatgct aagaaaaaca gacctacttc ccagtcgatc
    11461 acaaaagcca tgaccactga cccgatactt aaaaacggtg ctctgatcaa taaacgaatg
    11521 accattgcta aagctagctg aagctgattg atgtcgtttg tcattcgggt gatcaaggta
    11581 tctgttccaa aatgattcaa ctcgttgtga gagaaagtat tgattttttt gattagctga
    11641 ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag catagtattg gcaaatgata
    11701 gcacaaatca gaccaatcac agacatagcg accatccata agcccatttc aatgattttt
    11761 cctcggtcat tcatttttag tccattatcg atcaaggaag ccatcattaa aggcaggaca
    11821 agttcgaaga cagcttcaag aaatttgaag aacgggccca aaatgatttg cttacgatag
    11881 tcttttgcat aacgtaataa ctgaatcata tgattcctcc tattatttat ttgtccacaa
    11941 ttgaaaaaac aagtactacc acattgtagc ttaggaaatt tagaatgaaa agagaacaag
    12001 ccttttcata aatgaaagtt ttattatgaa gaagctttag aaattgaata gactattttt
    12061 tttaaaaaaa agaaaat
//
LOCUS       DAZUMT010000088        11967 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000088 DAZUMT010000000
VERSION     DAZUMT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.089"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            315..584
                     /gene="rpsN"
                     /locus_tag="ACG8FM_002192"
     CDS             315..584
                     /gene="rpsN"
                     /locus_tag="ACG8FM_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5094839.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            599..778
                     /gene="rpmF"
                     /locus_tag="ACG8FM_002193"
     CDS             599..778
                     /gene="rpmF"
                     /locus_tag="ACG8FM_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5094840.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            810..1187
                     /locus_tag="ACG8FM_002194"
     CDS             810..1187
                     /locus_tag="ACG8FM_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094841.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1208..1357
                     /gene="rpmG"
                     /locus_tag="ACG8FM_002195"
     CDS             1208..1357
                     /gene="rpmG"
                     /locus_tag="ACG8FM_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5094842.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1381..2364
                     /locus_tag="ACG8FM_002196"
     CDS             1381..2364
                     /locus_tag="ACG8FM_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5094843.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2394..2516
                     /locus_tag="ACG8FM_002197"
     CDS             2394..2516
                     /locus_tag="ACG8FM_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5094844.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2534..4057
                     /locus_tag="ACG8FM_002198"
     CDS             2534..4057
                     /locus_tag="ACG8FM_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5094845.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4081..4320
                     /locus_tag="ACG8FM_002199"
     CDS             4081..4320
                     /locus_tag="ACG8FM_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094846.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4513..5091)
                     /locus_tag="ACG8FM_002200"
     CDS             complement(4513..5091)
                     /locus_tag="ACG8FM_002200"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5094847.1"
                     /translation="MKHEHMDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRG
                     KTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRK
                     SCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNM
                     PFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5169..5387)
                     /locus_tag="ACG8FM_002201"
     CDS             complement(5169..5387)
                     /locus_tag="ACG8FM_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094848.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5576..6523
                     /locus_tag="ACG8FM_002202"
     CDS             5576..6523
                     /locus_tag="ACG8FM_002202"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5094849.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6490..6579
                     /locus_tag="ACG8FM_002203"
                     /pseudo
     CDS             6490..6579
                     /locus_tag="ACG8FM_002203"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6712..7119
                     /locus_tag="ACG8FM_002204"
     CDS             6712..7119
                     /locus_tag="ACG8FM_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS5094850.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(7360..8700)
                     /locus_tag="ACG8FM_002205"
     CDS             complement(7360..8700)
                     /locus_tag="ACG8FM_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5094851.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(8976..9131)
                     /locus_tag="ACG8FM_002206"
     CDS             complement(8976..9131)
                     /locus_tag="ACG8FM_002206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFS5094852.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9138..9284)
                     /locus_tag="ACG8FM_002207"
     CDS             complement(9138..9284)
                     /locus_tag="ACG8FM_002207"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094853.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9315..9518)
                     /locus_tag="ACG8FM_002208"
     CDS             complement(9315..9518)
                     /locus_tag="ACG8FM_002208"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5094854.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(9428..9760)
                     /locus_tag="ACG8FM_002209"
     CDS             complement(9428..9760)
                     /locus_tag="ACG8FM_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5094855.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10274..10465)
                     /locus_tag="ACG8FM_002210"
     CDS             complement(10274..10465)
                     /locus_tag="ACG8FM_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094856.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10563..10949)
                     /locus_tag="ACG8FM_002211"
     CDS             complement(10563..10949)
                     /locus_tag="ACG8FM_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5094857.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            11643..>11967
                     /locus_tag="ACG8FM_002212"
     CDS             11643..>11967
                     /locus_tag="ACG8FM_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS5094858.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMAD"
BASE COUNT         3991 a         1857 c         2277 g         3842 t
ORIGIN      
        1 aataaaaagt tatagtttct ttcatattgc cgttttaagt agaggaaaag ctacttaaaa
       61 cggtatttgt gtttgattgg actaataaaa gtgaattctt atttttcaga gcttaaactg
      121 catattcttt taaatgattt gattatctct atgtaccgat aaattcgttt tcaaacagtt
      181 atctattcat atgagtttta tttcatgtta tttattgttg acaagagcaa ctcttgttgg
      241 taaagtatca gtgtcaaaac gtaacgatta cgatttgaga tgtctaaatc aaaataaaag
      301 gagtataaag gtatatggca aaaaagtcga aaattgctaa agcaaagaaa caaatggcga
      361 tgattgaaaa gtacgcagat aaacgtcagg aattaaaagc cgcaggagat cgcaccgctc
      421 tcgctaaatt gccaagagat tcaaatccga atcgtctgag gcttcgtgat cagacggatg
      481 gtcgtccaag agggtatatg cgaaagttcg gtatgtcccg tattaagttt agagaacttg
      541 cccatcaagg gttgattcct ggagtaaaaa aagcaagttg gtaaaaggag gaaatatcat
      601 ggcagtacca gcaagaaaaa catctaaggc gaagaaacgg ttaagaagaa ctcatcaaac
      661 tgtagtaaaa ccagaaattt catttgacga agcgaatggt gattatagac gtagccatca
      721 tgtatcttta aaagggtact ataatggaaa gaaagtaatt aaaggagctt caaaatagtt
      781 taatatataa cttttaggag ggaatcaaaa tggaaattat ttacccacct ttagttgaag
      841 aaggattaaa gtattatctt gaaacgaccc agcaatcgct agataaatgc actttttatc
      901 gttcgatggt agagagagga attattactg aaacgggttt accgacccaa caggcaattg
      961 aaaatggatt agtcaaagat tactatgagg accaaggctt atcatttgat gattttttaa
     1021 gaatctatcc aatttttgaa gagtatgacg aggagttatt tcaatgtatt gatggttatt
     1081 gggagatacc tgttgatatg aaagaaaatt tagtttcaca attggaatct ggcgagttaa
     1141 cttttgaaga tgcacaacaa attcaagcat atcttgaaga tcgataagta ttgaaggagg
     1201 aagatttatg cgtcaaaata ttattttgga atgtgtcgaa actggagaac gtctgtatct
     1261 caccagcaaa aataaacgga ataaccctga acggttagaa ttaaaaaagt attcgccaaa
     1321 attgcgcaga cgggcaattt tcaaagaagt gaaatagcta agtggtaaaa aggtgacaaa
     1381 atgggcatac cagttacaat cgtttctggg tttttagggg ctggaaaaac aacgctaatc
     1441 aatcaagcct tgcaagtctc accctacccc agggaagaaa ttattataat tgaaaatgag
     1501 tttggtgaag ttgggattga tcacaaatta ttagttcata gcgaggaacg ggtgttacaa
     1561 ctaaataatg gatgtatgtg ttgcagtctt cgcagtgatt tattagcaat attgatttct
     1621 ttactggaaa caatagaaga acatcaacaa ccaatttcac aaatcatcat tgaaactaca
     1681 ggtatagcag atccccaacc aatcattcaa acacttttaa cagcgcctca actaagagga
     1741 agagtatata tcgatagttt attgactgtt attgatagtg acagttttga acttattgaa
     1801 agagaaccac aagcccttaa gcaacttgtc atagcagatc gcatttttgt gtctagaaaa
     1861 caaaacttga atttatctgc actaaaaaaa ttacagagag aaatcaaaag tattaaccct
     1921 ttatccgata ttcgaatgtt cacacatagt gaaacgattc aaaaaaagga tttttataat
     1981 ttggaaaaat tcaatcatcc cttagttttt gaaagtgatt catcagaaaa tcactataat
     2041 ggcaaacact atcatcaaca tcatcaagaa catgagttta aatcaattct cttaacttca
     2101 gatcaagaca taaaaaaaga ttttttattc caattaattg accggctgat tttaaccaac
     2161 caagggagcc tgtatcgttt taaaggattg attgctttgc aagatcaaga ttttatagtt
     2221 gccatacaag gggtaaacga acaggttaat tttcaataca ccactcgaca aaaagaccaa
     2281 accactatta ttctaattgg aaaaatgttg gatgaagaaa ggatcaaaca gagttttgat
     2341 aaattactgg cggaaagcaa ataatgataa gtattgatta ggggtaattt ctaatggaga
     2401 aaacggatat atctagtgca tatcgacgac ttaaaagccc gaatatcaaa actagaaaaa
     2461 gggcattaaa aattatcaaa gaacataaac aaaataaaat gaagaaatta gcataattaa
     2521 ggaaggaaaa agaatgaaga aaatcagtgt aggacttatt gggatagcag ctctaggatt
     2581 attgggagct tgctccagca ctaatgatgc aaaagtatcg aatgataaag atggaaaatt
     2641 agaaattgta acaacattct atccaatgta tgatttcacc aaaaacatcg tcggcgatga
     2701 agcgaacgtg gatttaatgg ttccagcggg atcagaacct catgactatg agccctcagc
     2761 taaggatatg gcaaaagctc atgatgctga tgtttttgta tatcataacg aaaacatgga
     2821 atcatgggta ccaaaagcta aagaaagttg gaagaaagct gggcctaatg tagtagaagg
     2881 aacaaaagac atgattttat tgccaggtag tgaagaagaa gatcatgatc acggagaaga
     2941 agatcaccat catgaactag atcctcatac gtgggtttca cccaaaatgg caattaagga
     3001 agtttcaaat atcaaggatc agttagttaa attgtatccg aaaaaagcaa aagtatttga
     3061 gacaaatgct gaaaagtatt tgactaaact gaaacggttg gatgctgact ataccactag
     3121 tcttaaagaa gcgaaacaaa aaagttttgt gacacaacat gcagcctttg gatatttagc
     3181 tttggattat ggattgatac aagttcctat tgctggacta agtcctgaag aagagccatc
     3241 ttctggtcga ttagctgaat taaaagagta tgttaaaaaa aataagatca attatattta
     3301 ctttgaaaag aatgctaatg ataaaatcgc aaaaactttg gccaatgaag ctgggataaa
     3361 attggaagta ttgaatcctc ttgaaagtct tacaaaagaa caaatggata atggtgaaga
     3421 ttatgtttca gtaatggaag acaatctaaa agcgttagag aaaacaacta tggttgctgg
     3481 tgaagaggta gttccagaaa aagaagcgaa ggatgaaaag acagttgcta atgggtactt
     3541 taaagatgcc gatgtgaagg atcgcgaatt gagtgattat acaggcgaat ggcaatctgt
     3601 ttatccgttg ttaaaagatg gaattttgga tgaagttttc gattacaaag caaaactcaa
     3661 taaagacatg acagctgccg agtacaaaga ttattatact actggctata agacagatat
     3721 tgatactatc aacattaaag acaacaccat tgattttgta gtcaacggtg aacatcatca
     3781 atacacatac aaatatgttg gatacaaaat tttgaattat gaaaaaggaa accgtggtgt
     3841 tcgttttaat tttgaaactg atgatgcagg tgctggtcgt tttaaatata ttcaatttag
     3901 cgatcatgga attgcaccaa gtaaggcaga gcatttccat attttctttg gtggagaaag
     3961 tcaagaaaaa ctttataatg aaatgcataa ttggcctact ttctatccag cttcattatc
     4021 tgagcatgag attgctcaag aaatgatggc ccattaaaat ccaatctttg gaggaaatac
     4081 atggtaagaa aagtattatt gtctatggat tccaagaata ttgatgctta tcggcgttat
     4141 caatttgcaa agcaaatgag attaaatggg agtacattag ctgatattgc tgcaacttta
     4201 catgttagta ttgacacagt tcatagagta ttaaaacggg atcctgattg ttgttttatc
     4261 aaggtcaata ctggaagaag tagacgttta gcaaaacggc ataatagctt ggccaattaa
     4321 ttgataatac cagagtagat catttttccc ttatttggat ttgatctgct ttttattatt
     4381 tcttgaattg tcaaatcaag cgcaacactt tatcaaagaa gacttcataa ggtgttctcc
     4441 aatctaaaca ctttctaggt ctgttattca gtttttcaat catctgattt atcgttgagt
     4501 tatctaccgt agctatctct ttcgattttg gcaagtattc tcgaatgagt ctattcgtat
     4561 tttaatttgt tcattgttgc caagggcatg aggatctgga aaataaaagg gcatattatt
     4621 caatacttct gtaactgcga aatgattgga gaattctttt cctctatctg gagtgatcgt
     4681 tttgaccgcc tcatttggaa tactgcttaa aagttcaatc attttctcgc tcacataagc
     4741 ggctttcttt ctggggacct tctttcctgc taaaaggaag catgattttc tgtctgtcaa
     4801 agtcactaaa caagtaccac tagttttgcc agctacaata tcggctttcc aatgaccaaa
     4861 atcacttcta tcatctgcgg attttggatg gccatggatc gaatgagtaa ggcaaatctt
     4921 cccacgagac tcgacatgat tttttgtatg tcttgttttt cctcgatggt gcaatgaacg
     4981 gatcaggcct ttattgccgt gtgagagggg ttctgtatcg aaaagatcat tgcaaattgc
     5041 acggtagatc gtgataaaac taattgaaaa gtccatatgt tcatgcttca aacgattcga
     5101 gattttctct ggcgaccact gaaaatcgac aaataaatga cgaacaattg accaaactca
     5161 cggatcattt agaatgtgat aacgtccgca tttttttctt cgataagcat aatttttttc
     5221 cgctttggaa ggtgattagt ttttttcttg tttcttctca gttcatgtaa aatagtaaac
     5281 gggctatgct gaatgtcgtt gccaattata ctaatagaaa atccttcttc gtgaagcaga
     5341 aagattcttt ctcgtttgag tatagtaaga tgacaatact ggctcataat tattttcctt
     5401 ctaggagtgt tctcgacaaa cttatactag ttggatatcg attatgactc atttttatgt
     5461 gttgcactta aagtgtaaat tcaaggtata gaaagaggtt ccctatgaaa aaatacctat
     5521 tacaatctta actggctatc ttggttcagg aaaaacgata cttctaaatc atattctgta
     5581 cggtgatcac ggaaaggaaa ttgctgtcat tgtaaatgac ataggggaag taaatgttga
     5641 tggggtatta gtcgacaaga aaggttttaa aagaactgga gaaaaacttg tttcgatgcc
     5701 gaatggatgc atttgttgta cattacgcga agatttgatt gttgaactag atcaattagt
     5761 gcaattttct gacttgaatc aaattgttat tgaagcttca ggaatcagca aaccaattcc
     5821 aattattcaa gcaattatat tagctaaaaa ccctttagac attgatttga cccaacgatt
     5881 agagattggt gccattatta tagtagtgga tgtaaaacgt ttttgggata atttcgaaac
     5941 gggtgaaaag attcaggaaa gaaaagctga aggtaatgta gttgaagaag aacaggatat
     6001 tcgtggcttg ttaattgatc aattcgagtg ttgtaacata ttacttttaa ataatagtga
     6061 cttagtgtca accaagacga tttacaaaat taacggtcta attgctaagt tacagtccaa
     6121 tgccaaaata attaggacaa ttaaaggtga agtagactta tcgaaaattc taaatacaaa
     6181 tctttttaat atagatgaga cttcctattc aatgggctgg ttcaaagagt tagagcttgg
     6241 ctatcaaaat catattcctg aaacagaaga atatggtatc tcaagtttta tctatcgaaa
     6301 tcggttactc cttgacgcac aaaaatttgc ccatcttacg gataaaaatt ttcctggcaa
     6361 tattacaaga gcaaaagggt ttctttggtt tgcccaacat agtgattttt cgacgatctt
     6421 tgaaacatcg ggtagccata aaagtattga atcattaaat tattgggtag ctagtttacc
     6481 taaaattgag tgtcaaatga ttatacaaaa aaacccgaat tgaaggaagc ctggcatccg
     6541 ttgtttggtg aacgcattaa taaactagtt tttattggta tgaagatgga tcaacaatgt
     6601 attaaggaac aacttgaggg ttgtttaact gattctctta atgcagataa tttccgaaaa
     6661 atttcaccat ttccatggaa aaattctaat atgtttagcc agatatcgtt aatgaaattt
     6721 ttaagaaaag ctggtgtcaa gataggcgat gtcataatta ttggcatttt gattattccc
     6781 tcttttattc ccttatttgt attctctata gaaaaggaga atgaaacaac tgaagatgct
     6841 atcaaatatg ccatcgttca aattgatggc aaggaaatgg atcgcttcga tttggataag
     6901 atcaatcata aactcgtgaa ttatcatcct gcgaagggac aatataatat tgttgagatt
     6961 aaagatggaa gaattcgagt taaagaagat aatagtcctg atcaaatcgc ggttagaact
     7021 ggatggatct caaaacccgg tcaaacgagt atttgtttac cccataagtt agttattagt
     7081 attgagaaaa aagaaagcaa agattactac atttactaat ctggaaatag atgaggaggt
     7141 ttttaataag aaaaagatat tattattaat aatagcaggg gctttattaa gtgtgttaag
     7201 cgcttgtgga caagaaaaag aagcggtgac aacaggcaca agtatcttta acgggacctg
     7261 caatggttta gttaacagtc ggttagatta cttattttgg ctcttcgtca atgagtgttg
     7321 gtgaagaaat tcaatagaat aatcgagata ctcacacggc tagacagcta tcttgtctag
     7381 ccgtgttgtt gttctggctt tagtcagttg ggtagtgttc ttagttttct tgaaatattt
     7441 tcctcgatat aaaaagattc gttctcggta gttttgaaag tttctaaagc cataggcaat
     7501 tcgttttagt actttgatat ggttgttcaa acattctata gggccgttgg aatagggaac
     7561 tcgcaacgct cgtttgattt ctgttcgata tttcttgaag gtcgtgaaca ctggttgata
     7621 gtgctctggc aactccttgt agtcttcttt taggagcgcg acgaattcag gaacatcttg
     7681 tcttctgata gcggaaagaa aaacctggta gacttcgtag cctcgcttta aggagtcatc
     7741 ataatataat agccgatcga ctatctccgc ttctgtcaga tgatctcgaa atgacggacg
     7801 ccagtagcga tgctgaccat tcaattccca cgcattcttt tgaagtagtc tccagtattt
     7861 ctttaagtgc ttcgaccgac gatccccttt tttaaaggtc ttcattactt gaattcggtg
     7921 gttggtaaag gcccgaccga tgtgttggct aatatgaaag cgatcggcga cgattcgggc
     7981 attcggaaaa cactctttca ccaatgtgcg atagggtgta tagaaatctg aaaccactag
     8041 acgtacttgt tcacggactt ttctagggta gcgcaagaaa tagtcgcgta aaggattgag
     8101 ttgccgattt tccacaatat caatcagttg atgggtcttg ccgtccatca tgacaaagct
     8161 catggctccc gagacgtttt tcaccgattt gaattcatcg aaacacacca cttctggtaa
     8221 cggctggttc aaagaaacgg ttttaggccg aaggctgcga agtattcgaa tgacagtggt
     8281 cggagaaatg tgcttcatac gtgcaatcaa agacatagaa ataggctcac ttaatagctc
     8341 aagaatggct tgtttgactc tgcgggcaat ggagcagcga cgatcgctaa ctgaatcttc
     8401 tgctaaaaaa gtctttccac agtgtttaca tttgaagcgc tgtttgttaa tccgcacaat
     8461 ggtcttgtag ttactgacat cattcaataa gatggatact tttttccagc cccatttaat
     8521 gatcgtttgc ttattttgat agtggcaatg gaagcattcc tctggttgat aggtcaatgt
     8581 gccatcaaaa acaaaacatg tttcgtcatt aatgcgctct ttgcgaaagg catcttcatg
     8641 aaaggtaaga tttaggtcta gaatatctag taactctttg gtaaactctg aataggacat
     8701 cttgaaacac tcctttgaat ggttttcgtc gactttattc taaaggattt caaggtgtct
     8761 gtttgcgttt attagaaaaa agaaaaagta ttggcaacag aaggcgtcac caacactaga
     8821 tattatagat cccttatttt cttcgcttca aattgtcaag gagtcatcat aaattttgta
     8881 tggattcttt tgtttgggta gtagtcttct aaataatctt ggattaacac cttcatctct
     8941 attttacttt tatatttgtt ggagtcgatt aattctcatt tgattgatcg atgaaacgct
     9001 tcaattactg cattgtcaac aggagtccca gtttggctca ttgaatgtgt gatgtggtgg
     9061 tcggtcaaca gctcttgata aataaaagac cgatgctggc tacctctatc ggaatgaatt
     9121 accgtaggca attctcctta ataaatttgt aatggacgga cgacaatctc ttttttcatg
     9181 tgtgaatcca ccatatagta acgtagaata cgagttgcta agtcaatata ggtggacaaa
     9241 tagaggcgac cgtcattaca tggaatgtaa gtaatatccg tcacccaaac agcatcaatt
     9301 tggttctgtt caaattatcg attcaacatt ttttttacca tttctttgat tgttttttct
     9361 ctaccatgag aagatttgcg tcgatgaaat cctcttgtgc ataaattcat ttcacgcata
     9421 agaacagcta caacacgttt attgatttcg attccttcat cgtaaagttt ttgagtgatc
     9481 cctggagatc cgtatcgatc tttgtgttcg tagaacactc gtcttcctct tttagtcgtt
     9541 tattttcctg ttcaagaatg tagaaatctg ctttatcagg taatcttttt ccttgtccag
     9601 caaaagcatt tttcccgtat cgacgatatt ctgaaaccca tcgggaaatt gttggacgag
     9661 agatattgta ttcttcagca attttcttgg tagttacttt tttctcaagg atatcctgtg
     9721 aaatctttag tttaaattta cgatcatatg tttttttcaa tagagttcat tccttccggt
     9781 aacgtaactg atagtcaaga aaagtgtatc atagcccaac ttagtatcag ggtttgtcaa
     9841 ctaaactgtg gaagttgata gataagagtt tttaacaacc aaaattctct tgactatttt
     9901 tgaaatcaga caaattttga ttggacacat agttgaataa atctaccgtc gttacggaag
     9961 gtaaatcgca tgcttttcaa ttctagagga gaagtcctcg ttgaagtacc atggcccagt
    10021 tctggattcg acctcctgcc cacttcgtac gtaaataaag taattttagg agtgagactg
    10081 tgacaaaagt tatttttaag ctaaaaacga attaaaaaac gttaataatt aaaatcattc
    10141 gttttagatt ctatgaatca ttatagaacg ctgtaaaatc gatactttat aattataaag
    10201 aagatggctt caaatttatt gttcgaaatt tgaaactatc tttttactta tgtcacagac
    10261 tcccttagtt gagttattct ttaatttcga atttcgagat agctagatat attgcaagca
    10321 tacattcttg ataacaatct tgatcatatt ttccagttct ccaggattgc ttatggacca
    10381 aagcgctaaa tcttttgaga atggccacaa ctgcttcttc atttccattc tttgctaaaa
    10441 tgaaatcttc ttttaaagta ttcataaatt taccttcttc ccatgaactt ataatctatt
    10501 tattttttgg ggaaggagga ggacgagcaa agaagcatac aaaattagtt attatgcaga
    10561 tattaaacca gcattttttg aatttttttg taaagtcgat gttttaaatt tgtcgcaccc
    10621 tgtcttgtaa tacccagaac ctttccaatt tctttatcag ttttattaaa gataaatttt
    10681 tcgataagaa aaatttgttc aggttctttc aacaaaggaa ggatttttgc taattttata
    10741 tcttcaacaa ggagagtcac atttaaaaca ttaatcggtt gtttaaattt tatagtatct
    10801 ttagtaaaca aatatggtgg gagttgatca agttgttcat gtttttgaat ttcttcttgt
    10861 tttttataat aagtattagc ggtgttcaaa atgacctttt caaagaatcg aatcaactgg
    10921 gcatttacgc gttcgtcgtt atctgacata ttgcacacct cctccattaa gatttaaagg
    10981 gaaaagaggc aaaaaacacg aaaagaagct aatgcaatta tgctgcgctg cgaataactt
    11041 ctataagctc acatgcattg ataggactgc tgatgtttat tttcaaaaat gtttttttag
    11101 ttgataatgg atagttatct ttatacttag aaaagaaaat tcatatatct tcttaaatat
    11161 caaacatgtc cttatggcta agaaaatgtc ttgagctttt tacgtataat aaatcttttc
    11221 taaaacctcc ttttggataa tattcttatt cgtgtataga ttgtattttt tatttcttcc
    11281 cccttcataa ttaacaacta gaaaagagtt cgaatagaca cgtatttgaa cgaaaatttt
    11341 tattggattc ccaaattgga gtatttagaa acgaaaaatt cctattatct gtatagtgct
    11401 tagataataa cggatcataa taattattgt ctttgaacta gatatatgta aatagtattc
    11461 atcatgatga aaaatgatga gttctcggtg ataaaatgta ctttttacgg tggaatatac
    11521 atgaaagcga taagtggata ttttataatg aagtaagtta gcttattttg acagaggaat
    11581 agtattttag ataacgtata aaatactatt taatgatgta gaaaggaaag agagaaacaa
    11641 aagtgttttc agataaagaa gtcttattaa agtctcttca tgtttgttta aagttaccta
    11701 tcgtggtttt taattccgaa ttcagtatta taaaagagta tcgctcagat agaactactt
    11761 atttttttta tgattataaa aagttactaa ctgataatat taaaaaaact aaagatttcc
    11821 atttctttac tggtgatttt aatgagatgt ttcttctata catgtttaaa accagatatt
    11881 acttatttgg tccatttaga gcaaataata ttgataaaga tttttttaaa ctaaaaatga
    11941 acaatttgaa tgttgcaatg gctgata
//
LOCUS       DAZUMT010000089        11960 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000089 DAZUMT010000000
VERSION     DAZUMT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.090"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..888
                     /locus_tag="ACG8FM_002213"
     CDS             7..888
                     /locus_tag="ACG8FM_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5094859.1"
                     /translation="MLLPGALWLFIFCYIPMFGVVIAFKDFRISSRGFFTSLFNSPWV
                     GWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSLAIILSQMANKRLLKKMQTF
                     MIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPINWYSEPKYWPFIIVFTSLW
                     KGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNVVLPHLRSIIVIMLILSVGS
                     IFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGDIGMSTAAGLYQSSVGFVLI
                     LITNKIVNKLDPSSALF"
     gene            900..1811
                     /locus_tag="ACG8FM_002214"
     CDS             900..1811
                     /locus_tag="ACG8FM_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5094860.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            1835..3307
                     /locus_tag="ACG8FM_002215"
     CDS             1835..3307
                     /locus_tag="ACG8FM_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5094861.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            3358..3969
                     /locus_tag="ACG8FM_002216"
     CDS             3358..3969
                     /locus_tag="ACG8FM_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5094862.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            3999..5912
                     /locus_tag="ACG8FM_002217"
     CDS             3999..5912
                     /locus_tag="ACG8FM_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS5094863.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            6040..9021
                     /locus_tag="ACG8FM_002218"
     CDS             6040..9021
                     /locus_tag="ACG8FM_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS5094864.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            9018..10679
                     /locus_tag="ACG8FM_002219"
     CDS             9018..10679
                     /locus_tag="ACG8FM_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS5094865.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            10719..11651
                     /locus_tag="ACG8FM_002220"
     CDS             10719..11651
                     /locus_tag="ACG8FM_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5094866.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         3903 a         1903 c         2474 g         3680 t
ORIGIN      
        1 tttattatgc ttttaccagg tgcgttatgg ttatttatat tttgctatat accaatgttt
       61 ggagttgtaa tcgcttttaa agatttcaga atttcatcaa ggggattctt tacaagtttg
      121 ttcaacagtc cttgggttgg ctgggaaaat ttcaaatatc tctttgcttc gcaagatgct
      181 tggatcatta ctcgcaatac ggttgtttat aacgttgtag gtattttgct aagtactttt
      241 tgtacagtgt cattagcaat cattctgagc caaatggcga acaaacggtt attgaaaaag
      301 atgcagacat ttatgatttt tcctaatttt ttatcatggg tagtcatcag ttactttgtt
      361 tacgcatttt tgagtacgga taaaggatta attaatattt tactaagcaa ttttggtgtg
      421 gaacccataa attggtatag tgaaccgaag tactggcctt ttattattgt ttttacgagt
      481 ttatggaaag gcgtaggcta tggatccatc atttattacg cgtcaattat ggggatcgat
      541 actagttatt atgaagctgc tttagttgat ggtgccagta aatggcaaca gataaggaat
      601 gtagtcttgc cacatttaag atcgattatt gtcattatgt tgattttgtc tgttggtagt
      661 atttttcgag ctgattttgg attgttttat caattgccaa gaaattcggg ggctctttat
      721 gatgtgacga atgtattaga tacttatgtc taccgtggat taatgaatag cggagatatc
      781 ggaatgtcta cggcagcggg attatatcag tcatcagttg gctttgtctt gattttgatt
      841 actaataaga tagtcaacaa gttagatcct agttcagcac tattttagga ggaatcaaaa
      901 tgatatcaaa attaaaacgg cagatgtact tttcgctgcc agtcgaacgg tttttcacaa
      961 tattactaat cttatttact cttagctgta tttttccctt tattttcgta atcgtcattt
     1021 cattaacaga tgaaaaagca ttacaactca acggttacga attattgccg gctcaatgga
     1081 gcactgatgc atatcaatac ttaatacaag atggcggaca gttacttcgg tcattaggcg
     1141 ttacaattat gattacagtg attggaacgt tgattactgt ttttatgacg ggaacctatg
     1201 cttatgtttt atcaagagcc tctttccctt accggaagtt ctttactttt tatctttttt
     1261 ttaccatgtt atttgcggga gggatggttc cttcttactt agtgatgacg aagatgcttg
     1321 gcttaaaaaa tacaatttgg gctttgattc tacctcttgc ttttagccca tacaatgtca
     1381 ttattttaag gacattcttt aagaagtcga ttccagaatc gattatcgaa tccgctaaaa
     1441 tggatggctg tagtgagttt cgagtttttt tccaaatcgt tttgccttta gcgatcccag
     1501 gggttgcaac aataggttta ttttcaagct taggttattg gaatgactgg ttcaacgcat
     1561 tgctgtatat tgacaccaac aaattgattc cattgcaata tttgttaatg aagattcaaa
     1621 attcaatgga attcttagca aataataatg acattacatt agcgcagcaa caagcaattc
     1681 aaaatagcct tcctcaagaa tcaacacgaa tggcaatggt cgtagtagct actttaccga
     1741 ttgcaattgt ttatccgttc ttccaaaaat actttgttca aggattgacg atcggtggag
     1801 tgaaagaata aaaaagaata ggagatgtta aaagatgaaa aagaacatgc agaaatattt
     1861 gattggtggt gcttctgtac taacccttgg cctattaggt gcttgtggta gcagtaatgg
     1921 cgaagcaaat aatgaaacag gaaccacaaa agttacttgg tatcaggttg gagaagaacc
     1981 caaagatttt gatgaggtca tgacaaaagt caacgaaaaa ttaaaggaag aaatcaatgt
     2041 agagttggat atgcggctga ttccggacgg ggattatcag caaaagcttg gtgtgatgat
     2101 caattcagga gaagaatatg atatttgttt cgtcaatggc acagattatg tgaattatgg
     2161 gaacaaaggt gctttcatta gtctcaacga tcatttggat aaagagttga aagattatgc
     2221 agctgaattg aatgaaggat ttattgaagg tggggcaatt aatggcgaaa cttatggatt
     2281 gccagtcaat aacgatattg ccacaacgga atattggacc tttaataaag atttggtaga
     2341 tcaataccaa ttggatgtag agaatgttga aggtgtagaa acattggctc cagtattaga
     2401 aaagtttgca gaaaaaaata ctgatacgaa tattacgcca atcggtatga cccgtaccag
     2461 tgtctgtatt ccttatgaca atattatcgg tggtggtgta ccgctagcgg ttgcatttga
     2521 aggcgataca caaacagttg ttaatactta tgagactcca gaaatgatga cttatttgaa
     2581 ggctatgcat gagttttata aaaaaggata tattttgaaa gatgcagcca ctgttacgac
     2641 agatccattt cgaattgata gtaaaaactg gtttttaggt cgtaactcaa gtggttatca
     2701 accattagca caaaaaaaat tagaagctct agcgggacat ggattaattt taaaaccagc
     2761 tggaaaagct tttaaaacga ctggatttgc aagagtggca atgcatgcgg tatctgcttc
     2821 aagtaaacat ccggaagcag ccttgaaatt gttgaattat gtgaatacca atcaagaagt
     2881 agcggattta atggtttatg gaattgaagg aaaacactat gatcgcaatg aagatggttc
     2941 gattacttta acaaactctg atgattatta tcaaggcgct tgggcattgg taaatactta
     3001 tctagtttca aaaacttcac cgattacaga agaaagtaaa aaactagaag aagaaagtaa
     3061 aaagtttcaa aatgaagcac cgatgtctcc tattttaggt ttctcttttg acgcttctga
     3121 ttttcgaaca gaaattgcaa gtgtacaaaa tatcgtgtca caatatgaag tacttttaag
     3181 ctcgggaaca gttgaccctg aacaatatgt accagagttg ttagaaaaac tagaaaaagc
     3241 tggattggat aaaattcagg catctgctca agaacaatac gatgaatggc gaaaatctaa
     3301 caaataattt tttactaaat gaaaagaata gactaagcag ttagtggggg gattcaaatg
     3361 aacgaaaaca aatggaccaa aggatttaat tgggtttttc aattgttgtt actaaacttt
     3421 acttatttta tactaatttg tttaggattt ggaattttag gttttttccc tgctacgata
     3481 gctttgattg ctggcgttgt gaatcgaaaa aaatatggct tgcaagagtc tgtgaagttc
     3541 atgagtaaaa tctataagaa aaattttgtt agatataatc tatattgctg gtcagcaact
     3601 ttgttgttgg ccggcaatat tactacgatc tggctttgtt ggcagcaaag cgcaccattg
     3661 ttttatttat taagtacatt attatcggta attacactgt tcctagtctt tgcaatgttg
     3721 aattgggtcg tgatcaaaag tcttgggttg tggatgatgg gagaccgact gattgactat
     3781 ttgcttttca cagcaacgat gttgccaacg gtagtcatgc aaggcttggt gcttggaatt
     3841 tatttccgaa tattgatgat tttgcctgtg atttttctgt ttggcggaac agctgtaacg
     3901 gtaatattga ttagtaaatt aactgaaaga gcgttagcaa agttgccttg tcagatacaa
     3961 tcgcactaat tttttcacaa aaaggagtat ttctttctat gaagttaact gataaacaag
     4021 tagctaatat agcctttatc cagcaacttg agattccatt gacacaaaaa gatgaagaac
     4081 aaatagactt gatttttact gacacaaagg ctaatgtgcg acaaatcaag attactaaaa
     4141 atgagcagca acgaatttgc ttgtcttatt cacatgaaga cttgctgtat cgaggattgt
     4201 ttatctgcct tccacgtttt ttagctcaag gaggagagat gccatacatt gaagaaacct
     4261 cgatcattga gaccagttat tcattagatg cttctcgtaa tggcgtgatg aataaagaga
     4321 ctctaaaaaa attcatttct tacttagcgt tgatggggta caacacattg tatatttaca
     4381 cagaagatac ctatgaagta gaagaccttc catattttgg ttaccttcgc ggagcgtata
     4441 caaaagaaga agtgaatgaa attgttgaat ttgcttctgg ttttggcata gaagtagttc
     4501 catcgatcca gacccttgct cacttgaccc aatttttgaa gtggtatgct caacaagaac
     4561 tgatggatga tcaaaatact ctactgatcg gtgaagaaaa ggtttatgcg gcaatcgaaa
     4621 agatgattat tgcctgcaaa gaaatgtatc agacagagcg tattcatttg ggaatggatg
     4681 aagcctatca agcaggtctg ggacgatatt taagcctgta tgggtattgt gatcggacaa
     4741 ctttaatgat tcaacatatg gaccgtgtgt tatcaattgt tgataaatat aagatgaaac
     4801 caatgatttg gagtgatttc atttataagc tattagatga aaccggtaaa gataggtatt
     4861 acaatcctga ttttaaactc aaagcattag cagctaaagg attaccagca ggattaacct
     4921 atgttcattg ggactatggt tcagatgaag tttcctccta tgagaaagta attgataacc
     4981 atttgaaatt ttgtgacatt gatcattacg ttttagctag tggtgcccat atctggggga
     5041 gactagcccc gaatcatggg aaaacagaag caattgtttc tgcagctgta aaagcttcta
     5101 aaaataaagg tgtacgttct gtgatgttaa ctacttgggg agatgaaggg caggaaacag
     5161 agcattggca ttccttaatt ggggcattaa aattagcaga ttatgtctat tctgaagaag
     5221 agtggaatga ttttgaggaa atctacgatc gtttattagg aacaggtagt tatcaattaa
     5281 tgtccgaatt acgatatttc gatgaagtgg ataccattac aaataacaac ttacaaatgg
     5341 cttccgtgag taaactgcta ctttggcaag atccgttagt tggtatatat gactttcatg
     5401 tagccgctca taacaaaatt gccactagaa caattggtac ttattatcat gagttagctc
     5461 agaaattaga tatgaataaa aattggaatc caaaacaacc aattttaaca actattcgtc
     5521 aacgatatat acttttagct gaaattttag cacaaaaggc agaactaggc atctatttac
     5581 aacactgctg tctggtagaa gaccttgcag gattaatgga attagcgaat cggtgtgaag
     5641 cactagccaa tcgagtaaat gaattggcat ctattcatga aataatttgg tatcaaacat
     5701 acaaaaccta tggttgggaa gtattagaac aacgatatgc tgcattagct catcgtctga
     5761 aaaccgttgc gcgtcgtatt aaaaatcatc ttcaggggca agatgcccta gaggagcttt
     5821 tggaagaacg attaccgttt tgtcaatatc aagtgccgct tgaaatttct ggctttaatt
     5881 atcggcaaag ctcggtgtca ggctacaatt aacgaaggaa tcttcccgtt gaacggacca
     5941 cttgctcatc taatgggaaa gtggttcgtt ttctattcat aagtagctta ccactaactc
     6001 ttttattaag aagagaaaaa aagtaggaag tgagaaaata tgttatcgga ctattatcaa
     6061 aatctgtcag ttataagtgt caatacagaa ccattacgta attattatat tccttatgca
     6121 agtaaagaag aagcaaaaca agagttagaa agggaaaaaa cgagccagtt tatcgatctc
     6181 aatggtacat gggattttca gtattatcct tctgtccaac agttaccgga aaattttttt
     6241 caagaggcct ttactgaaag ctatgataga atttctgttc ctggagtttg gcaaacagag
     6301 ggatttgact tacctcagta tatcaatgta tcttatccaa ttccttttga tccgccttat
     6361 gtacctaatg ataccccttg tggtgtatat cggcgtcagt ttcaaattga gcaaacagat
     6421 gcacgctttt acttaaatct agaaggagca gatagtaatt gttttgtttg gataaataga
     6481 aactttgtag gctatgacca agtgagtcat agtacatctg aatttgatgt gaccaagtat
     6541 ttacaggttg gggttaatga aattcaaatt ctagtgatga agtggtgtga tggtacctac
     6601 tttgaagatc aggataagtg gcgaatgagc ggtctatttc gtccagtgta tcttcttcaa
     6661 cgaccaacta tgcatctgac aagttatcaa ctacggacgt tgactgatcc taaaaaagga
     6721 agcggaacat taatggtttc ttttcaacga tttactccta ttgaagtatc gttacctatt
     6781 tcttacgaat tattccatcc tgatggacgt ctcatagcga aaggggagtg ttactcggaa
     6841 caacaaataa atttggatgt tgaagattgc cttttttgga cagcagaaac gcctaaatta
     6901 tatacattac ttttaacatg taatggtgaa tattttaaag aaaaagttgg ctttcgagca
     6961 ctgacgatca agaatcgtaa gttattgctg aacggaacac ctattaaatt atatggagtc
     7021 aactatcatg acgtgaatca gcaaggcacg ccagtgatga cccgagagca gttacgtgca
     7081 gatttgttat taatgaaaaa attaaatgtg aatactgtac gaacggcaca ttaccctaaa
     7141 tcaccagatt tttatgaatt ggcggatgag attggtctgt atgtaatcaa tgaagcagat
     7201 ttggagtgtc atggtgtggt taatctatat gatactgaag cgaattatca actaattgct
     7261 aatgatccga cattcgaaaa gccgattatg gaccgagtga tccgttctat tcttccttat
     7321 caaaatcgaa gctctatcat tatgtggtct atggggaatg aatctggtta tggagtaaat
     7381 tttgaaaaag ctcaagcttt agcacggaaa ttggatgatt cacggttgat tcataatgaa
     7441 cgctcgattg aaccagttat tgaccaagtg aatgatttta gtaacttgga tgttattagt
     7501 caaatgtatc ccccactgga agatgttcat cgtcattgtc aaagaagtga tactgaaaaa
     7561 ccttttttta tgtgtgaata tagccacgct atgggcaacg gtcctggaga tcttaaaacg
     7621 tacttcgatt tgattcaaca gtatgattcc tttcttggcg gttgtgtctg ggagtggact
     7681 gatcatgcaa tcaatagagg aactttagaa acgccatctt atttatacgg tggtgatttt
     7741 ggtgaaaaat atcacgacgg caatttttgc gtagatggtt tattgacccc agatcggcaa
     7801 ttaacagtag gtgcattgga gtaccgtaat attcattcgc ctattcaact gattgctgta
     7861 gatgaggagc tgcaagaagt aaccttgagc aatataactg cttttacagc cggttcagca
     7921 tttaaccttt ctcttgaatg gtatatagat ggaaaaaaaa cagtttatga agtaatgaat
     7981 cctttcatac ttgagcctgg caaaagttgt tcattgccca tcacagtacc tacaacgatt
     8041 catggtgctc atacggttgc tgtttcgcta tctgtttata aaaatgatga gctttatgga
     8101 cagcaacagc tctttcttaa aaaagaaaaa atgagattgc caagaactgc tcatcataat
     8161 gatgcaccac aaatttcagt aaaaggcaca gagctgataa tcaaaggaga atcctttatt
     8221 tatatatatc aattagcaaa agctgcgttc acttctgtaa agatcaatca aaaggaactt
     8281 ttggaacgtc ctactcaatt ttcaatttgg cgtgcaccaa cggataatga tcgtaactta
     8341 gtaaacaaat ggaaagcagc tggatatgct gaaatgtctt tagaaatcag aaaaaaagac
     8401 tggaaattcg tagatgatca tctcctgatc gaaacgtctt atcgaggagt tattccagga
     8461 ttacagccaa tttttacaat gcacatacag ttgaaaataa atggtgcagg cgtaatcaac
     8521 caaaaagtat cagtggagaa aaatccgatt tttccagatt tccctcgctt tgggatggac
     8581 tggccgttga atgatacttt ttcgaaggtt tcttattgtg gctatgggcc gtatgaaagt
     8641 tatcctgata agcactatgc tgcgatgttt ggtatttatg agatggagat tgaggaattt
     8701 gatcagccat atatcaaacc acaagaatat ggtaaccgct ggcaatgtga ttgggtatca
     8761 atcaaaaatc atcaaggatc taccatgacg gtttatggaa actctaattt ttctgctctg
     8821 ccttatagtc aagctcaatt aaccgaaaag tcacaccatt atgaattaat aaaagaaaat
     8881 agaacttggt tcaatttaag ttataaacaa aatggtatcg gctcacacag ttgcggtcct
     8941 tatttagaag atcgtttccg ttttatggaa gatacttttg actggaatat agtgattact
     9001 ttcaaaggag aaaatagatg aaagaaacaa agatcaatca agtcaaaaaa aaattatctc
     9061 gttatcaaga agttgtttat cgaaaccatt gcttaaaatg gtcaaaaaat aagctgaatg
     9121 ttcgttttaa tggattattt tatcgtttcg aacagttaga agaagtctgg aaaccggtaa
     9181 cgaatttcca atcatgtcct gtacagctca taaatgatcc aaaagaaaca tacgatttat
     9241 gtataacaca cctttataac cagcatttga aaaatgacgg attttattta aaggttacta
     9301 atgacaatca acttacgatt gaatcagcaa accttcgagg gtttcaatat gctatggaag
     9361 ccttattaga gagttttttt tcggtaggag atcaattatt attaccggaa ttgattctga
     9421 aacatgaacc atcagtgaaa attcgaggaa tcattgaagg gttttatggt atcccctgga
     9481 cacatgaaat gcgcctctct cttttttcat atatgaaaga taaccaattg aatactttta
     9541 tgtatgcccc taaagatgat gaattattac gtaaaaaatg gcgtgaactc tacgatgcgc
     9601 aagaattaga caaatttaag gagcttttat ctgctgctga ggctagcaat atcgattttt
     9661 ggtacttaat tagtcctgga aatgatattg atattacgtg tgaagaagac attcaagttc
     9721 ttctgaaaaa gttagaacaa ctgattgagc ttggcgtatt tcaatttggt ctattaatgg
     9781 atgatattga ctatcaatta aaaggtgctg ctaaacgccg ttttcgagag ccagcattcg
     9841 ctcatgccta tttagtgaat cgagtagagg aatatcttag cactcggttg tccaatcatg
     9901 aattagtcat ttgtcctaca gaatatgata atcgtcatgg ttctcgctat ttagcaacgt
     9961 taagtcaaga aataccagaa caattaccgt tcttttggac tggcccaagc acgttagcgg
    10021 cgtcaatttc tacagaagag ctgcaggaaa tggcttctgt ctatcaacgg ccgatgctga
    10081 tttgggataa tattccggta aatgattatt tagaggataa agaactattg ttcatgagtc
    10141 catatgagaa tcgaacaccg aatctttcta aggagagata ccaggttaca ggagtagttt
    10201 cgaatccaat ggctcaatta gaagcttcaa agtttacgat caatagtatg gctaattact
    10261 tatggaactg tgagcgtttt gatcctttgg aaacttggac gtcagtcgtt gaaaaagtag
    10321 ttggaagtaa actacaacca gcttatctta ccttaaccaa tgcttttcca aatcattaca
    10381 caagcagtct gctttcagat gaagaattac tgctagccaa agacccagaa tgggtcacaa
    10441 aagaaatggc tgctttagtc caagcagtaa aatggatcca gcaagaacaa catacatcta
    10501 gattgagaac agaattggag ccttggttac aagcaatcac agaaagttgg actttctggc
    10561 aagaatggca attaaaaaaa gatccaaaag aagcagaaga atggttggct aagaaaaaaa
    10621 cacaccgcat aggacgtgat ttagtaactg cccatttaga gcaaattatc aaaagttaga
    10681 tatcttaaaa aataaaaagc aagaaaaagg agaacaaaat ggtcaagaga ttaattagtg
    10741 caaactacag tgatgtcaag aaaatgacgt caaaagagtt gaagcaatcc atcaaagcga
    10801 gtgaaggacg ggtgattgtt tgtgaaacgg ttgctaatac tcttccacaa tctggagaaa
    10861 ttacgaatgc agaagttgcg tcagcatttg gtgcagattt gattctttta aatggtttcg
    10921 attgttatcg tcctgcagtt tttggactac caaatgtgac gattgagaag gtttttgatc
    10981 tacagcaaac gattgaaaac ccgattccta aattaaaaga actagtcggt cgtcctgtgg
    11041 gaattaattt agaacccatt gcccctaaaa caaatatgat gagtgagaaa gtagaaatca
    11101 gccaaggaag agcaagctct gttgaaacga tacaaaaagc agaagaacta ggagtcgatt
    11161 ttatttgctt taccggtaat ccaggcactg gagtaaccaa tttagaaatt actaaatccg
    11221 taaaaatggc aaaaaagtat ttttcgggat tgattattgc tgggaaaatg catagcgccg
    11281 gctcagaaga gccagttgtg aataaagagg cgattatttc ttatgcggaa gcaggcgcag
    11341 atattttact agttccttca gtagggtcta tcccaggctt tttagagcaa gacctaattg
    11401 aagcggttcg gttggcaaaa aaatatgatt tgttaacgat gtctgcaatc ggtactagtc
    11461 aggaaagtgc ccgaccagaa acattgcggc aattagccat tatgaataaa agttgtggtg
    11521 ttgatttaca acatattgga gatgctggat acgggggatt ggcaccagca gaaaatattt
    11581 tcgaaacctc ggttgcaatt agaggaatgc gacataccat caactgcgtt agccgctcga
    11641 tcaatcgcta acaaaattaa ataaaaaatg gttaaattga ttatttaagt gggaacatac
    11701 ttgagatatc agctaacttc tgtcacttta aaaaagggac tgtgacagaa gtgaaaatag
    11761 acaaaaaata tccgaacaag agactaggat agcagaaaaa ctatcgtttt gcaacgagga
    11821 ccaactggac cgtaggagca attctcttat tgttcggata tttcgcttgt tcaaggactt
    11881 atgtctgtag tgcccttttt tggtaggaaa aatggtaggg aaccatatca tttttaatga
    11941 aaatcaagca tttcaaaagt
//
LOCUS       DAZUMT010000090        11874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000090 DAZUMT010000000
VERSION     DAZUMT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.091"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..265)
                     /locus_tag="ACG8FM_002221"
     CDS             complement(59..265)
                     /locus_tag="ACG8FM_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094867.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(281..895)
                     /locus_tag="ACG8FM_002222"
     CDS             complement(281..895)
                     /locus_tag="ACG8FM_002222"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS5094868.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1042..1725)
                     /locus_tag="ACG8FM_002223"
     CDS             complement(1042..1725)
                     /locus_tag="ACG8FM_002223"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5094869.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1834..2292)
                     /locus_tag="ACG8FM_002224"
     CDS             complement(1834..2292)
                     /locus_tag="ACG8FM_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS5094870.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2376..4139)
                     /locus_tag="ACG8FM_002225"
     CDS             complement(2376..4139)
                     /locus_tag="ACG8FM_002225"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS5094871.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4360..5820)
                     /locus_tag="ACG8FM_002226"
     CDS             complement(4360..5820)
                     /locus_tag="ACG8FM_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094872.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6028..6954)
                     /gene="glsA"
                     /locus_tag="ACG8FM_002227"
     CDS             complement(6028..6954)
                     /gene="glsA"
                     /locus_tag="ACG8FM_002227"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS5094873.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6977..8404)
                     /locus_tag="ACG8FM_002228"
     CDS             complement(6977..8404)
                     /locus_tag="ACG8FM_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5094874.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8435..9649)
                     /locus_tag="ACG8FM_002229"
     CDS             complement(8435..9649)
                     /locus_tag="ACG8FM_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS5094875.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9735..9962)
                     /locus_tag="ACG8FM_002230"
     CDS             complement(9735..9962)
                     /locus_tag="ACG8FM_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094876.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9990..10232)
                     /locus_tag="ACG8FM_002231"
     CDS             complement(9990..10232)
                     /locus_tag="ACG8FM_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5094877.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10264..10821)
                     /locus_tag="ACG8FM_002232"
     CDS             complement(10264..10821)
                     /locus_tag="ACG8FM_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5094878.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10834..11022)
                     /locus_tag="ACG8FM_002233"
     CDS             complement(10834..11022)
                     /locus_tag="ACG8FM_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5094879.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(11035..11601)
                     /gene="amaP"
                     /locus_tag="ACG8FM_002234"
     CDS             complement(11035..11601)
                     /gene="amaP"
                     /locus_tag="ACG8FM_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5094880.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3479 a         2450 c         1940 g         4005 t
ORIGIN      
        1 ataatttttt agttttatgt gtttttttat atgttttttc acttttcgga ataaatattt
       61 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
      121 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      181 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      241 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      301 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      361 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      421 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      481 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      541 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      601 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      661 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      721 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      781 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      841 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      901 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      961 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
     1021 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1081 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1141 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1201 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1261 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1321 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1381 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1441 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1501 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1561 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1621 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1681 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1741 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1801 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1861 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1921 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1981 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     2041 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2101 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2161 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2221 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2281 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2341 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2401 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2461 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2521 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2581 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2641 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2701 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2761 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2821 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2881 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2941 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     3001 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3061 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3121 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3181 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3241 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3301 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3361 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3421 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3481 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3541 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3601 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3661 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3721 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3781 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3841 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3901 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3961 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     4021 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4081 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4141 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4201 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4261 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4321 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4381 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4441 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4501 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4561 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4621 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4681 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4741 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4801 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4861 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4921 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4981 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     5041 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5101 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5161 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5221 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5281 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5341 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5401 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5461 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5521 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5581 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5641 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5701 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5761 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5821 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5881 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5941 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     6001 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6061 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6121 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6181 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6241 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6301 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6361 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6421 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6481 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6541 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6601 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6661 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6721 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6781 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6841 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6901 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6961 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     7021 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7081 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7141 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7201 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7261 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7321 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7381 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7441 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7501 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7561 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7621 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7681 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7741 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7801 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7861 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7921 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7981 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     8041 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8101 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8161 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8221 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8281 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8341 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8401 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8461 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8521 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8581 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8641 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8701 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8761 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8821 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8881 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8941 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     9001 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9061 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9121 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9181 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9241 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9301 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9361 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9421 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9481 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9541 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9601 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9661 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9721 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9781 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9841 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca
     9901 tataaaatat ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc
     9961 atttcaacat aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact
    10021 aatactaaaa tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt
    10081 ccccaagttc ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt
    10141 gcgatgatcc ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata
    10201 ccgccgacga ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac
    10261 ttcttattgt acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc
    10321 agattttgct ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt
    10381 tactttgtct tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc
    10441 aacattcaca ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat
    10501 caattctttg atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc
    10561 tagatccaca gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc
    10621 tgtgttgact aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc
    10681 atctacatct ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa
    10741 ttcaccattc atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt
    10801 attcatgttt ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat
    10861 tcctgactgt ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa
    10921 caatgtcttg aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc
    10981 aaagataata gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc
    11041 cactcgagag tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac
    11101 tgccactttg accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg
    11161 catcaataca cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg
    11221 gatatggatt cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc
    11281 ttcgtgtaaa tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc
    11341 atcttcccgt ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa
    11401 gatgataata gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta
    11461 gtaattcgtt aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc
    11521 attgatcaac gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat
    11581 tatttttaac cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa
    11641 ctctaccgac aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg
    11701 ctaccaaaca ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa
    11761 ttgcttactt attctctatt tacttttgat tgctggctat acttaaagga tattattcaa
    11821 gattaaaaat tgcaaagatt atgctcagtt attattacaa gattctattt attt
//
LOCUS       DAZUMT010000091        11564 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000091 DAZUMT010000000
VERSION     DAZUMT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.092"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(311..2320)
                     /gene="metG"
                     /locus_tag="ACG8FM_002235"
     CDS             complement(311..2320)
                     /gene="metG"
                     /locus_tag="ACG8FM_002235"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS5094881.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2588..2866
                     /locus_tag="ACG8FM_002236"
     CDS             2588..2866
                     /locus_tag="ACG8FM_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094882.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2963..3784
                     /locus_tag="ACG8FM_002237"
     CDS             2963..3784
                     /locus_tag="ACG8FM_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5094883.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4069..5079)
                     /locus_tag="ACG8FM_002238"
     CDS             complement(4069..5079)
                     /locus_tag="ACG8FM_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5094884.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5091..6044)
                     /locus_tag="ACG8FM_002239"
     CDS             complement(5091..6044)
                     /locus_tag="ACG8FM_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094885.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6034..7104)
                     /locus_tag="ACG8FM_002240"
     CDS             complement(6034..7104)
                     /locus_tag="ACG8FM_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094886.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7110..8153)
                     /locus_tag="ACG8FM_002241"
     CDS             complement(7110..8153)
                     /locus_tag="ACG8FM_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5094887.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8154..9095)
                     /gene="opp3b"
                     /locus_tag="ACG8FM_002242"
     CDS             complement(8154..9095)
                     /gene="opp3b"
                     /locus_tag="ACG8FM_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5094888.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9179..9526
                     /locus_tag="ACG8FM_002243"
     CDS             9179..9526
                     /locus_tag="ACG8FM_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS5094889.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9596..11257)
                     /locus_tag="ACG8FM_002244"
     CDS             complement(9596..11257)
                     /locus_tag="ACG8FM_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5094890.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            11436..11555
                     /locus_tag="ACG8FM_002245"
     CDS             11436..11555
                     /locus_tag="ACG8FM_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5094891.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         3278 a         2337 c         2095 g         3854 t
ORIGIN      
        1 taaagtcatt gcattcaaac tttatgaatg taaggacaat aaaaaagagt accagatgaa
       61 tcaattaaaa tctgcccgac cctttatcat tatgcttttt ttctgttttt acgattccta
      121 ttagtattat tctaaattaa caaaaatcag cgaagctcgt acttcttttt ctaggattta
      181 ttttggatag ttctctgttt ctgtattata tgattggctt caagtaataa gaaggaattt
      241 acgaaaattg cttctcaaat ttcggaaaaa gggggtatga cacaactagt gtcatacccc
      301 ctttgaattt ctatgcgatt tctgagccat ttggcatgga ttttggtgct tcgattactt
      361 gtaaagagcc atctggtgct tctgcagaaa ggatcattcc ttgactgatt tgtccacgca
      421 tctttcgtgg tttcagattt gctacaatga cgactttttt cccgatcaat tcgcttggat
      481 caggataaaa ttcagcgatt cctgacaaga tttgacggtc ttggctgtct ccagcatcta
      541 gtcggaattg cagcaactta tctgcccctt ctactttttg acagttgatg acttctgcta
      601 cttttagttc tactttttca aatacatcaa atttgatttg tttttctttc gttgaaacaa
      661 gttctgtttc ttctggatcc catttgactg tatcttcgtt tgtttgagta ccttcagaca
      721 ttttttcttg aataaaagca acttcttctt ccatatctaa acgtgggaag attggtgtac
      781 cttttgcaac tacttttgtt cctgaaggaa attcgccaaa gtgtaatcct tcaagattca
      841 ttgtttcaga gtccaatcct agttgattga agatcttctc tggcgtttct gtcatgattg
      901 gctgaagtaa tatcgctgcg atacgcaaag attcagctaa atggatcatg acactattca
      961 attcagcttt cttttcttcg tcttttgcta atacccatgg ttctgtttca tcaatgtatt
     1021 tgtttgcacg agaaatcagc acccagattt cagccaatgc tgtattgaat tccatttttt
     1081 ccattgcatc atggtaacgt ccgacaacgt tagcagccgt cgtagacagt tcactatcaa
     1141 atggagtgac aagtgaggca tagtcaggaa cgagtccatc acaatatttg ttgatcatag
     1201 caatcgttcg attcaataag ttccctagat catttgccag atcataattg acacgggaaa
     1261 caaaatcttc tggcgtaaag acaccatctg acccaaacgg aacagcacga agcaaataat
     1321 aacggagtgc atctaaacca taacgttcca caagcatttc aggataaacg acatttcctt
     1381 ttgatttaga catttttccg tctttcatca atagccagcc atgcccaaat acttttttag
     1441 gtagaggcaa atcaagggcc atcagcatga ttggccaata gatcgtatga aagcgaacga
     1501 tttctttacc gaccatctga acatctgcag gccagtattt ttcgaataga ctttcatctt
     1561 cggagccata tcctagtgca gtaatataat tggataatgc atcgatccat acatacacca
     1621 catgtttcgg atcactcttg actggtactc cccaagtaaa agttgttcgt gaaactgcaa
     1681 gatcctctaa tcctggtttg ataaagttgt tgatcatttc gtttttacga gattctggct
     1741 ggatgaattc gggatgttct tcatagtatt ccaatagacg gtctgcgtat ttgctcatgc
     1801 ggaagaagta tgattcttct tttaccaatt ctacttcgtg tccacttggc gctttaccac
     1861 cgatcacgtt accttcgtca tctttgtaca cttctgcaag ttgtgtttct gtaaagaatt
     1921 cttcgtcaga aacagagtac cagccttcat attcacctaa gtaaatatct ccctgttcta
     1981 ataaacgatc aaatattttt tgaacagctg ctttatggta atcatctgtc gtgcgaatga
     2041 atttatcata actgatatca agcgttttcc ataatttctt gacatcagcc gccattttat
     2101 ctacatattc ttgcggtttt acacctagtt cttctgcttt tgtttcgatt ttttgaccat
     2161 gttcatccac tccagtgaga taaaacacat caaagcccat caaccgttta taacgagcaa
     2221 ttgcgtcaca tgcaatcgta gtatacgagt tgccgatatg aagtttgccg cttggataat
     2281 agattggcgt agtgatataa aaagtttcct tttctgccac ggggctcttc ctcctttatc
     2341 gcatgatgcg aattcgtttc cttgctagta taccataaaa aaagcatgct gttagctttt
     2401 tttcaaaatg aaaaagactg gataaacgac ttctatctac gttcaatcta tcctttaaaa
     2461 ggatgaatag gttgaataaa tgcgggtttt tgttttgttt catatctact aaaaaacggc
     2521 tttttatttc tcccctcaca cgtggtataa taaagactat ctatcttagt aagggactgg
     2581 aacaatcatg cagaagaaac atcttttttt tacacttagt attgcttttc tttcattagc
     2641 acatttaata ttcagctatt tttatattcg tatgtatggt tattttaacc tccatgggca
     2701 tttgaacagt tttatgacag cagcttggat tttgcgtttt atcattgacg tatatatcgt
     2761 tatttgtggc ttctttgcca ttcgtgaaga gcggtataaa gtattgccgt tttatctgct
     2821 tttcttttta ttcaatctca tacttccatt tatttttcat atttaaatga taacctagat
     2881 ttttcaagta aatccttggt tttttttcag agtctgctac aataaggtaa aaagtacttt
     2941 tactgtcaat ggaggtggtc agatgaaccg ttttgcttct aaagaagaag aaaaattata
     3001 ttatgaacat aaagcaagtg aagaagaatt tcttaactgg tatcaaaaac aggaacgtcc
     3061 agaatatgac aagccctctt tgactgtcga tatcgtactg atgtgctata acaaagaaga
     3121 agatcagctg aaagttttgt tgatccaaag aaaagggcat ccttatcgaa attcatgggc
     3181 attgcctggt ggctttgtcc aaaaggatga gtcaactgga gaaagcgtct taagagaaac
     3241 aaaggaagaa actggtgtgg ttatttcaaa agaaaatatt gagcaactgc atacattcag
     3301 tacgcctaat cgagatccgc gaggctgggt cgtcacagtc agctacttag cctttattgg
     3361 agaagaaccg ctaatcgccg gagatgatgc caaggaagta cgctggttca cactagaacg
     3421 tcatagtaac aagatccatt tatctagcgg agaagtggct atctcgcttg atttagtcac
     3481 tggagagtct tctggcaaag atactttggc ttttgaccat agtcaaatca tcttgaaagc
     3541 attcaaccgt gtagtcaata aaatggaaca cgagccgcaa gttctacaag ttttaggtaa
     3601 agatttcacg attacggaag ctcgaaaagt atttgctaaa ttcttaggga tcgattataa
     3661 aacgatcgat cattccaatt ttaagaaagc tatgctccaa tatttcgatg agatcggtga
     3721 acgtcctgta ggaatcggcc gtccttcaaa aatctatcag ctcaagccgg ggcaccatga
     3781 ataaggttgt gagcaaaaga ttcagaccta atcattcaag aaaaaacgca aaaacaactc
     3841 tttgtgagtt acgttttttg tttcagaatt ttgatgtatt cgtaaacttt ctgaataaaa
     3901 aattaacaat agcagaaaaa gctcgtacga actttttctg ctattgtttt attggtttgt
     3961 cataattagt gagcttttct tcaaaagaaa aattcctaga aacaaaagtc attaagactt
     4021 tttattaaca aggctaaccc taacgctcct atcagtttca ttcatttttt atcccttgat
     4081 aaccgaatgt ctgcccattt tttctactac ttggctcttt cttacggaat tgctttcttc
     4141 tgctccgtcg gtattgatca aaagaaattt tttattatac cattgggact tataacgcaa
     4201 ttttgttatt aatttatgcc agtcgtgaat agaaaaatca gcatgtgcct cttcttttgg
     4261 aaaagattct acaaataagt agtcatattg tttgaccaat ttcccagata attgttcaag
     4321 ataatcttct cgctggttct tttgtttcag ataaagatcc attacttttc tcttttgttt
     4381 ctgataattg cttgcctgat ctaacctgat ctttctatgg tgagcggagc ggactttcac
     4441 attcaatttc cgctgtgcat gttgcagctt ttcttctgtc tggtgaaatt ttggtaaagt
     4501 tacttctaga ggtcgagatg tttggatcaa tgtcttcgga tgataggcga tcccgatcca
     4561 tttagctaaa gattcttttt ttactggcga aacatcacaa agtagtgcga cataaaattc
     4621 ttcattatat cgagcagaaa tcgtagcgga gcgaatcgat ccttcaactg gccgatgctg
     4681 atttatcttg attagttctt tttgttttgg taactttaag tagccatttt ccagatagat
     4741 cgtacgggtt tggttgttgg tcgtataaga ttgccacata ctttttttcg ttttaagctt
     4801 tgggaagctg gcacgtccct tgaaataatt gcgaaaagcc cgatccaaat taagctgtgc
     4861 atttgctaaa gcaagactgt ctacttcttt taagaatggg tattcttttt ttaatgtagc
     4921 gggtgtgagc gaaaaattgg ctactcctgt ttgttgccaa cttttctgcc gctttttcag
     4981 caaatggtta taggtaaatc gagcacatcc aaaagtacga atcaattgct gtttttgttc
     5041 ttctgtcgga tacatacgga atttaaaagc ttttagcatc tatgtccctc ctagttagca
     5101 accagaagag attccttttg tcgttttagt ttttttgcgt aatatgggac ttcctgctca
     5161 gttgcataga caaaatgttc tggtgcgatt tcacgaagtt gacgtacttg tccgtcatct
     5221 ggttcaggtt gatatttgat tcttctgcgt tttctctcat ggtctggatc tggtagagga
     5281 atagcagata acaaactttc tgtataagga tgaacaccgt gattatagat ttcatcactt
     5341 gttcccatct ctaataacaa gccgttatgc attacaccga ttcggtcact aatatgttta
     5401 accatcgaca aatcatgtgc aataaataga taggtcaatt gatgttcttt ttggagatct
     5461 tgcaacaaat tgaccacttg ggcttggatc gacacatcta gtgcagaaat tggttcatca
     5521 caaatgataa aacgcggttt tactgccaat gctcgtgcaa taccaatacg ctggcgttgc
     5581 ccacctgaaa attcatgagg ataacgagta ccgtggcttg gattcaatcc aacagttctc
     5641 aatagatcat cgactttttt tgcacgctct tcagatgatt tagctagtcc attaacatca
     5701 attccttcgg caatgatatc tctgactttc atacgaggat tcaacgaagc gtatggatct
     5761 tgaaagatca tctgaacatc atgccggaat ttcgtcaaac cttttttatc tttgatcttc
     5821 gttatatctt gaccgtcgaa taatatctca ccatcagtag gttcattcaa gcgaatgatc
     5881 gtacggccag tagtggattt tccgcttcca gattctccaa ctaaaccgaa tgtttctcct
     5941 tcataaatgt gaaagctgat gtcatttaca gcatgtactt cattcttttt tcctacattg
     6001 aaatactgtt tcaatccttt aacttcaagt agtttatttg gcatcagcaa ttcctccttg
     6061 accgtaagta tgtttttgtt tctctttaaa gatagcccag cgacgttgga tttcttctgg
     6121 cggtgtcact ttgggtgcct gcggagcaag tagccaagtt gctgccttgt gtgtttttga
     6181 tagttcaaaa aatggtggtt cttgttctgc atcgattttc atcgcgaatt catttcttgg
     6241 atagaatgcg tctccttttg gcgggttcaa taaatccggt ggagaaccag gaatagcata
     6301 caatcgatcg ttcgcactat ctaatgtcgg cattgaacca agcagtcccc aagtataggg
     6361 atgttgcggg ttgtagaaaa tttcttccga tgttccaact tcaacgattc ttcctccata
     6421 catgacagcg acacgatcag cgacattcgc tactacacca aggtcatgag tgataaagat
     6481 aattgatgta tcgatttttg tctgaatttc tttcagcagc tccaaaatct gtgcttgaat
     6541 agtcacatca agcgctgttg taggttcatc agcaatcagg atttccggat aacataccag
     6601 tgcaatcgcg atcacgatcc gttgacgctg acctcctgag aactgatgcg gataactgtt
     6661 gatacgtttc tcagcttcgg gtattccaac taatttaagc aattctaatg cagctttttg
     6721 tccctctttt tttgaaactt tattatgctt tctaagggac tcagcgactt gcttcccaat
     6781 gggcatcgtc ggatctaaag aggtcattgg atcttggaaa atcatcgcta tttctttacc
     6841 acggattttt tgcatctctc tttctgattt tccaacgata tcgttacctt taaagaggat
     6901 ttgaccagca tctatattgg cgttgcttga cagcaagcgc atgatgcttc tggtcgtcac
     6961 tgattttcca cttccagatt ccccaacgat cgccaatgtt tctcctttat tcaaatgaaa
     7021 attgacaccg cgaatcgctc gtacctttcc agcatacgta tcaaaagaga tttctaagtc
     7081 tttcacttct aaaattttcg tcatcgtctt cactctttca ttttaggatc aaaggcatca
     7141 cgcaatccat cagcaagtaa gttaaagcca atcatgataa ccgataaagt tactgccgga
     7201 atccataata gataaggcag atacaggaat gtcttgtacc cttcgctcaa catcgttcct
     7261 aatgaagctg acggaggcgt aagacctaaa ccgataaagc ttaaaaaggc ttcgaagaaa
     7321 atagcagatg gaatactaaa catcatttga ataataatga cactagagat attcggcaaa
     7381 atatgtttga atgcaatttt cattcttgat tcacctaatg tcaatccagc taaaacatat
     7441 tcttgatctt ttaatttcat tgtttgtgca cgaacgatac gtgccattgg tatccagttt
     7501 gtgatggcca tagcagccac gattgaaaaa attcctggtt caaaaacgac caacatcaaa
     7561 atcatgacaa cgaggtttgg aattcctgat aagatttcca aaaaacgttg catcacgtta
     7621 tcgacacgtc cgcctaacaa accagaaacc aacccgtaag ctacaccaat cgttacatct
     7681 agtaatgcag caataaatgc gatcagcaac gagattctca ttcccataaa taagcggctt
     7741 aatacatcac gtcccaaacc gtctgttcct aaaaagaaat ttacgtttga agggacgttt
     7801 gcttgagcat atttatcaac aagttcacca ccaaccattg cttttccatt caatccatcg
     7861 atattgattc ccggaatacg aggaggcaaa ttgatataag ccacgttttg ggcagttgga
     7921 tcatgaggag aaacaaagat tgtgatcaca gagatcaaaa tgatgaccaa tagtaaaacc
     7981 attgaaataa cggcagcttt gtttttcttt aaacggcgcc atgaatcttg gataaaattc
     8041 aaagaaggtg cagtaatctg ttcacgttct tcttcggtat ttttttgtaa tggaagaaat
     8101 tcatccgcag ggatatctgc aactgtttca taacgctttg gtatcatttc catttatcct
     8161 cggctccctt ctgaaacacg aattcttgga tcaactagac cgtacaatag atccacgatc
     8221 aaaataacaa aaatcagcat aaatgagtaa aggatcgtta cagccatgat cgtaggataa
     8281 tcatttgttg tgattgactt aacgaattgt tccccgatac caggaatagc aaagatattt
     8341 tctaccacca gtgatccggt catcaatgct acagctaacg gtcctaataa agtcatcaat
     8401 ggaatcaaac tatttcgaag accatgttta aaggcgattt cccaacggct caatcctttt
     8461 gcttttgcta attccacata atcactatgc agtacttcca ccatttccgt acggataaac
     8521 cgagcagagt cagcaagcgg cgacatggcc aacgcaattg ttggtaagat tgtgtacaca
     8581 aagccatccc attttgcgat tggcaaaact tgtaatttga ttgcaaagat atattgtaat
     8641 aaaacagcaa atacaaagtt tggaatggaa cgacctaata tcgctacgag tgtagaagat
     8701 gtatctgccc atgtattttg cttcatggca gagatcgttc ctaaaatcgt ccctaggact
     8761 gttccaaaga taattgcctg taaccctaac tgcaaggatg gtccgattct tccagctaac
     8821 aaatgtgcga ctggctggtt tttaaattgg aaggaaattc caaaatctcc ttgaagcaaa
     8881 ttagaaagat aaatcccata ttgtacgata actggtttat ccaaaccgac ttgtttattt
     8941 agcatctcga ttgtttcagg actcaaacgt tcctgattcg tatatggcgt gcccggcagc
     9001 aattgcatca agaaaaaggt gattgttgca attaaccaaa gcgtgatgat catgaaaaag
     9061 acacgtttta atacatattt gatataacta ttcactaatg tttcccccta gcttttgact
     9121 tctgacagat gtttcgctac attattagat aatgaaccaa aaataaagga gcaatcaaat
     9181 gaaaaaaaaa tctatcccct acgcagtagc ttttctactg attctggtta ttttaataaa
     9241 aaatctcatc aaccattcct ttacactcat tcaattatct aatgatctat tcttatggtc
     9301 attgccgttt ctgattatcg gcggtttttt atgggtcttt tcttcgggtt tcttcgatca
     9361 ttttcagcga tcggtccatc ttgcacgaac aaggaaccga aagaaaaaac cagaattttc
     9421 cacgctctcg tctgccagct acggtatgta cagcttttgg ctgatcattg ccggaatact
     9481 aattgctctt tctgctattt tcatgctttt ttctttgtta ggataagtga atatgagaac
     9541 gaggttgcga catttgccgc agcctcgttt tttctcatgc tcacggtatt aacgattatt
     9601 cttcaacgta tgtccatttg taatcccatg aagcgcctgc cgcatggtga acgatacctt
     9661 tgattttttc attcactagg tgaccttctg ctttttggta caccggaacg acacccatat
     9721 catcagagat gatcttgatt gctttttgga aatcatccca acgggcttgt tcatcgccag
     9781 cgtctgttga tgaagctgct ttaactgctt cgtcatattc tgcattgctc cagcgaccac
     9841 ggttgtatga attacctgtg acaaacaaat cagtaaagct gcttgggtct gcatagtcag
     9901 cagcccatcc gcctagtact gcgtcaaaat cacccgaagt tgagcggtca agacgtacag
     9961 agaatggaac aggtgttggt ttgactgtta cgccatccaa gttttcttgg attgagtttt
    10021 gaacatattc gattactttt ttcgatgaat catcatcaga agccatcaaa tcaaattcta
    10081 aagaatcaac gcccaattct tttttcgctt tttcccaata ttcttttgct ttatctttgt
    10141 cataagaaac taattttcca gcttcttttg caaagtcttc gttcgttgtt ggacttttta
    10201 ccatatctga tgggatcaat cctgtagaga caactgatcc gtcacctagt acttgtttta
    10261 ctaaagcttc acgatcgata gaataagaaa tagcttttct taggttcaca ttgttgaatg
    10321 gtgaattctt ttcacgttga ttgaattcga tatagcttgt ccgcgcttct ttaacagaag
    10381 tataagctgg atcgtttgcg ttttgctgtg ctaactcacc agacaagatc acgtcatctg
    10441 cttggccatc tttgaataag ttcaatgcag tagaagattc ttttactaca ctgactttga
    10501 tttcagaaag ttttactgta tctttgtccc agtaatgatc atttttctta taagtccatt
    10561 ctgtatctgt tcctggtcca tcaaaatctt ctaatgtgaa tggtccatta taaacagctt
    10621 tatcacttgc agatgcatag gcatccccat tttcttccac tacggattgt ttttgtggga
    10681 aaaatgatgg gaaagcaagc agatattgga aatatggtgt ttgtttcgtc aatttgattt
    10741 ctaattcata gtcaccaact gctttgatcc ctagttctga tttatctttc tttccagctg
    10801 taatgtcttc tgcattttca actggcgcat acaggtaagc atattcagaa gctgtagctg
    10861 ggtctactgt tcgttgccag ccataaacgt aatccgctgc tgtaacagga tcaccatttg
    10921 accattttgc gtcttctcgt aatttgattt tgtaagttaa tccgtcttca ctgacttctg
    10981 ccaactcgct agctcctgcc ggctgtggtt tgctgtcttc atcaagacgg taaattcctt
    11041 cataaacgtt attcaatgct gtaaaactga ttgtatctgt tgcaactgat aagtcagctg
    11101 taggcatttc ttgaggcacg actaagttaa atacgcctcc tccatctgct gaaccgcttt
    11161 ttgctgaact actgcttgtg gctgaactgc cgccataaca tccagcaagt acaaggccgc
    11221 ataccgcaac caaaccaaat gtaaatttct ttttcatttt acgtacccct ttattggaat
    11281 tttcagaata attaatattt tctgaagttg tttaagagtt taatgaataa ttattaaaaa
    11341 atcaacactt ttttttgtct ttttcataaa ataatgattt tttcgtgagg tttcaaggaa
    11401 tttttcagaa aaaatagctt tgtacaaggt tttccatggt cttccaaaag ccattaaata
    11461 attttcgtta tattgtctat tattgccata caaagataaa aaagaataaa caaaaagaaa
    11521 ctgtgacaaa agtcttgtca cagtccctta tctgaataaa cggt
//
LOCUS       DAZUMT010000092        11556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000092 DAZUMT010000000
VERSION     DAZUMT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.093"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8FM_002246"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8FM_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5094892.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8FM_002247"
     CDS             complement(323..892)
                     /locus_tag="ACG8FM_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS5094893.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8FM_002248"
     CDS             1078..1980
                     /locus_tag="ACG8FM_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5094894.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8FM_002249"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8FM_002249"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS5094895.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8FM_002250"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8FM_002250"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS5094896.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG8FM_002251"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG8FM_002251"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS5094897.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACG8FM_002252"
     CDS             8548..8733
                     /locus_tag="ACG8FM_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS5094898.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACG8FM_002253"
     CDS             complement(8795..9991)
                     /locus_tag="ACG8FM_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5094899.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..>11556)
                     /locus_tag="ACG8FM_002254"
     CDS             complement(10161..>11556)
                     /locus_tag="ACG8FM_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5094900.1"
                     /translation="STEIMAGVTTFFAMSYILFVNPTILSASGMPFQAVFLATIIASI
                     IGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAMVFICGLINIFITVT
                     NIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQSAISSSVVEGGKATN
                     VTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIGIVATTVLGIFMGVV
                     DLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQVLMTIIAFSLSDTF
                     DTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIATSVGAIFGTSNTTT
                     YVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATAPALILVGVMMMASF
                     ADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVIKGKYNQVSVALWIV
                     DILFIINFIILATI"
BASE COUNT         3403 a         2469 c         2036 g         3648 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgat
//
LOCUS       DAZUMT010000093        11506 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000093 DAZUMT010000000
VERSION     DAZUMT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.094"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..376
                     /locus_tag="ACG8FM_002255"
     CDS             170..376
                     /locus_tag="ACG8FM_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS5094901.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(578..1579)
                     /locus_tag="ACG8FM_002256"
     CDS             complement(578..1579)
                     /locus_tag="ACG8FM_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5094902.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1584..3497)
                     /locus_tag="ACG8FM_002257"
     CDS             complement(1584..3497)
                     /locus_tag="ACG8FM_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5094903.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3665..4171)
                     /locus_tag="ACG8FM_002258"
     CDS             complement(3665..4171)
                     /locus_tag="ACG8FM_002258"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5094904.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4331..4771
                     /locus_tag="ACG8FM_002259"
     CDS             4331..4771
                     /locus_tag="ACG8FM_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5094905.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4797..5954)
                     /locus_tag="ACG8FM_002260"
     CDS             complement(4797..5954)
                     /locus_tag="ACG8FM_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094906.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5957..6313)
                     /locus_tag="ACG8FM_002261"
     CDS             complement(5957..6313)
                     /locus_tag="ACG8FM_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094907.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6611..7585)
                     /gene="sgrA"
                     /locus_tag="ACG8FM_002262"
     CDS             complement(6611..7585)
                     /gene="sgrA"
                     /locus_tag="ACG8FM_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS5094908.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7781..8620)
                     /locus_tag="ACG8FM_002263"
     CDS             complement(7781..8620)
                     /locus_tag="ACG8FM_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5094909.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8805..9692
                     /locus_tag="ACG8FM_002264"
     CDS             8805..9692
                     /locus_tag="ACG8FM_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS5094910.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLIDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10286..10981)
                     /locus_tag="ACG8FM_002265"
     CDS             complement(10286..10981)
                     /locus_tag="ACG8FM_002265"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS5094911.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(10965..11363)
                     /locus_tag="ACG8FM_002266"
     CDS             complement(10965..11363)
                     /locus_tag="ACG8FM_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5094912.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3433 a         2297 c         1722 g         4054 t
ORIGIN      
        1 gatggtaaac tttgtaccta tctgattgct gaatcattaa ttgcttatca ttagccatct
       61 ttgtttcaag ttcgctagat gatcatttta ttcacatcct aacaaacttg tcacatttat
      121 agattcttaa tctatttatc atacaattat aaaaaaggag gtgctgctta tgaaagctat
      181 ccatatttct ttgctttttt tcgctagtgc attgatttta tttgctattc atctttgttc
      241 ccagccttat atcaataaca acggaatatt agtggaacca gcattctttt gcttaccact
      301 cggctttttg tcccttcttg cctccgcagg cttcggattt gttgcttttt ttaaacgatc
      361 aaaacaacaa atctgactta taaaatttac gtaaaagaga tcgtgacaat aatcgtgtga
      421 tattcccaaa tttcgaataa aaggtgcccc ttgccatctc taactcctca gtcacaagtt
      481 acaatattca taaaccatga aaagcttgtt tattgaaatt gctccttgtg acttggagct
      541 aagtgacaga ttacaacctc ttttgcatca taagacttta ctccttgaaa aacattacta
      601 acttttgata tgtttcttgc gcttgtttat tgctgaaatc aaactgatat tcatggttca
      661 aaacagcatt tttctccgca taatctactt ttgtgacttt cacccccgct ttctcaaata
      721 cagaagttac ttcttccatt tgtttcgtaa acgtatttgt atttccatcc gttaagaatg
      781 tgttttgcgt ccaacgatcc gcatgtctga ccaaggctaa acttttgacc ttctctgatt
      841 gttcaaagtc cttattatca aagtaagccc atccacattt ttcataaaga aagctagaga
      901 ttttatcact tacttgagta agtgcagaag gatccaacag accagacaaa gaaataaact
      961 tcgtaatctg cttgttctcc aaagtcggat caagatgatc agctttttta atttcttcat
     1021 tttgaagtgc taacaaatat tctccactaa tttgtgcacc tgccgaattt ccaccaatcg
     1081 aaatattcga ccaatctgct tgatctttta tctctttttt agctgcttga atggcttcgt
     1141 tcaattggat aactggtgta ggatgttttg cttccggtga tcgttcataa tctatattta
     1201 cgatgatttg ctgcgtgtct gaacatagat attccaaata atcacgacaa tcatttttat
     1261 cccctcctac atatgcgcct ccatgcatcc agaaaagaat cggtaatttt ttcttttcca
     1321 ctttcggtgt gtagatatcc atattattat ttttgtactt tgaaggatag gatatttctt
     1381 tttttatatc gatttgttcg attttttgct ggaaatcatc tggtcgggca tatacttgtt
     1441 ctttttcact tgtgtcaaat tgttttctaa caagcaagct aatcggcttt ggtgtgattt
     1501 gattaatcaa aaatacagtg actaaaataa ctacgatgat aactggtact gcaatcacta
     1561 ttcgcttgaa ccatttcatt catttaccct ctctttcttc gccaaatcca tgtgataatc
     1621 gtgctaacag ataaaaatcc acctgaataa atgagaagcg aatgtttctt ttctccagtt
     1681 ttcggcaaca ttcttttttt tgcatctgtc tttttttgta ctttcactgt catctgaatc
     1741 ggttgcgtat catcacttga ttgatcctct cgaggttcat tctttccctt attgtctgca
     1801 ttctctgtcg gatcttgctt ttttggtgga gctactggga tttttttgta atagaaaata
     1861 atagttggta tcgtctcatt aaaaatccct tcagtcggtc cttcaacatt tgctaattcg
     1921 tatccttcga atgtcttttg ctctgtcata taattttctc cataagctcc gtaaagctgg
     1981 atttcgtcct ggtctttgag tagactttta ttgttttcat ccacatactt tacgtttatc
     2041 tgatcatgga acactacttt caaaaagtcc gttacatgtt tcgtgtaggc agcatggtca
     2101 acttcctgtg cctttccatg ccctgctcct tctactaata aaagatcttt ataccctcct
     2161 gataactcat ataatttttg cgcgttagac acaggtacta catcatctgc tgttccatgg
     2221 ataatcaatt taggcaatga atctgacatt ccaatcgtag gtagagcagc ttttgtatcc
     2281 aaatgcatct cctgatcaaa atagtattgt ttcaaaaggc ccataaataa atcttcatgc
     2341 tcttgatccc caatgatgtc taactgattt tttatggcat ccggtagtgc gggggctact
     2401 gtctggctgt acgtaaaacg tagttgttca tcaattgatg caaatccgca attttcaata
     2461 attcctttga cattgacagg aatatctttc gctaatacag acatcatcgt agctgctccc
     2521 atcgaaccac cataaagtaa aatcttttga gatggccgct ctcgaacttc ttgatttatc
     2581 cagtaaagga cgtcatctga ctcatattgt ccataggtca cataatcgcc tccgctatta
     2641 ccagtcgctc tggaatcaac aaataataaa ttatacccgg ccttataaaa atcattgtat
     2701 tcttctgtaa cgatcccgtt attccaattc cctcgaaatc cgccatggat catgactgtt
     2761 ttatctgaac cttgatcgac ataatatgca tatacatcca tcattttgcc atcttgtttg
     2821 tttagtacag tttgatgaag ttgttttgta tttggataga agcctgtgtt ttctgtaggc
     2881 gttgtcccat catctgtacc tccaagcatc gacatcaaca cactgttttt tgcaaaagcg
     2941 atatcgtaca atagataagt agcaatcaga tgtttgataa tctctttatc tacaccataa
     3001 tcattaacag caagctcagt gactaataac tctattaatt ttgacttgat gtcttctgtt
     3061 ggtccattaa gtatttctgt catctgttta ccgaagtaat tcaaatattc tttaccattg
     3121 aaattttcac ctttaccgat atcatcattt tctgtcccat tctctttgtc acttacttta
     3181 gcagaagagg attcgtcgtt tgacattttt gtagctgttg cttcttctgt ttgttggtta
     3241 tctgtggtag cggtcgtttc ttgatctgtg ctagtaacag atgtctcaac cgttgtttca
     3301 gtatttgttt ctgtttgtga agtactcgat ttcgtttcat ttccagcatc agtagtttct
     3361 gtggtttcta cctcccgaga ttccgattcg gaagaaatgc tctctccata tactgtccat
     3421 tgcgggacgc ttgtacttac agcaaataag atgaccaacg ctccctttaa tacttgtttt
     3481 gttctcaagc tattcactcc tttttatgtt tctaggagaa ttatatgtaa acgcaaacat
     3541 tttttataca aaaaataaat ggtttatccc ataaaaatag aacaggatta ttttctcatt
     3601 ttacgtaata aagcagaatg gataaaccag tatccattct gcttttttta aacgaagaaa
     3661 caacttattg ctccaaaaat gtcagaaatc gtttatccca aattttctcg taaaaagcga
     3721 ttgtttcctt tgctttcata aaaggattat cataagttgt cgttgctcct ctacacatca
     3781 taagctggta tcctaatcca tgagcaaact tcacagtcgt atctatacaa tactctgctt
     3841 gtgccccaca taattcgatc gaatgacagt ccaattcagt taataaaaca ctcaaattag
     3901 tcttataaaa tgaattcgca tgggtttttc tcacaaaata actgtgctca ggaatagata
     3961 attccgacaa tagctcccat ggttttgttt gtggcactaa ttgagcatct tcgtgttgaa
     4021 cgaaaataac tggtttccca atcgtttcgt atgttttgat tcttttattc actaatgctg
     4081 tcactgtttc taattcgtgt atcggcgaag aatcaaaaca tacccctttt tgtaaatcga
     4141 tcactactat tgcgtcggct gtgtttgaca tacttgtgcc ttctttcttt tttcttgatt
     4201 taatagtatt tcttatctag ctagttccat tatacgtgac aacattttct tattttacac
     4261 ctctaaatgg cacttttcga ctatttcttc atgttattat ttcataaata cttagctatt
     4321 ggaggaatgt atgatgatta aattgtatac aggttctggc tgctcgtctt caagacgcgc
     4381 aaaacgttgg cttatcgtcc atgatatacc gtttatcgaa cagaacttaa ttcgtcaacc
     4441 actaaccaaa aatgaattta tccacttact aaaattgaca aacaacggcg tgaccgaaat
     4501 gctctctact cattctatta gttataaaaa tcttgttcac agaatagatg aactcagttt
     4561 gagtgaacta tatattgaga ttcgtacatc cccttcttta ttgaaaaaac cgattatcta
     4621 cgaccaatct gcaaaacttt tgactggatt tagcgaagat aaaatcagga tgttcctttc
     4681 taaaactaaa agaaagcaag agctcaacga actcttgctt tctggtcatc ttgtcgattt
     4741 taaagaaaat aatctttttg caatgtctta attttggcat aaacaatctg cttttcttaa
     4801 ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat ttttatgatt
     4861 tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta aaatatctgt
     4921 gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac aatatcaggt
     4981 aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa tatccttttc
     5041 tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat gacaggttgt
     5101 tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa ccgtctgttc
     5161 ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa aaactgttgc
     5221 atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc tgattttcca
     5281 aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat cattgccaaa
     5341 ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt tttttcaaaa
     5401 ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc tggagtatcc
     5461 aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag gtaagcttgc
     5521 tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg ctctagtatg
     5581 taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat ctcttctggg
     5641 acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag taggctgatc
     5701 catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt ttctaatagt
     5761 tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa tgactgccaa
     5821 tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg attacctttt
     5881 ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct taattttctt
     5941 tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat tgataagtac
     6001 ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt atagaataaa
     6061 ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt tctgaaagaa
     6121 aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga atcaccgaat
     6181 tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca tataatttct
     6241 cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg atatcaataa
     6301 attcttcaaa caaagtacat actccttttt atctattatt tagacttatt ccgacagcta
     6361 catgaataac gtacaaatag attcgttact gatttttcag agatactacc atgcgcagtg
     6421 tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt tcctgtttct
     6481 caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca ggcaatacag
     6541 cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt agatctgttg
     6601 cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct cctacaatca
     6661 aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta ccagtttctg
     6721 gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca attccacgag
     6781 aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt ctcttttgtt
     6841 ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct gtgctgcttt
     6901 cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta ctttcactac
     6961 tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca ctactcgtac
     7021 tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc gtactctctg
     7081 tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta tccgtactgc
     7141 ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc
     7201 catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca atcgatagtt
     7261 ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct tttaaataaa
     7321 cttccccaat aactttttct gaacttccat taccatccca accatatgtt agaacagcat
     7381 gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg acttgttgtg
     7441 tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca tttgcccgtt
     7501 catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca agaataacaa
     7561 ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc ttctatttga
     7621 taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac
     7681 aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt
     7741 aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt
     7801 atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca
     7861 agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat
     7921 tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac
     7981 ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac
     8041 ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg
     8101 ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa
     8161 tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa
     8221 gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt
     8281 ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca
     8341 gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa
     8401 cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta
     8461 gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc
     8521 caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa ccaactaaga
     8581 ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat
     8641 taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat
     8701 agagagaata gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac
     8761 tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga aattttttga
     8821 aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta
     8881 gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt
     8941 atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca
     9001 aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata
     9061 atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa
     9121 cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag
     9181 ccaatgtaaa attaatagat aaagatctag acactgcttg gaaagaattc tatccagaag
     9241 ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag
     9301 caaatgaagg agaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa
     9361 aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa
     9421 aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc
     9481 caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt
     9541 ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg
     9601 atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat gtaactatta
     9661 aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg gggatgtggc
     9721 aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac cacttttgcc
     9781 acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat ttgttacaat
     9841 catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat gcgccattaa
     9901 gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa aacaactatc
     9961 ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc ccttctggac
    10021 ttgttcttct tattctgtta tactagagaa acacgaaaga tatttttatg cgacactttt
    10081 catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca aggatgtatt
    10141 agaggttagg gaatacatcg tttaaatgag aaataagtaa tagttagtaa tagtcaccca
    10201 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
    10261 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
    10321 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
    10381 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
    10441 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
    10501 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
    10561 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
    10621 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
    10681 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
    10741 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
    10801 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
    10861 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
    10921 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
    10981 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
    11041 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
    11101 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
    11161 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
    11221 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
    11281 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
    11341 acaaccgttt caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag
    11401 tttttattca aaatgtttct agattataaa atttattgaa taacacattc ctaacggaat
    11461 ttgtttcgat attgtgtgat ttctcagagt acaatggatg tatgga
//
LOCUS       DAZUMT010000094        11432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000094 DAZUMT010000000
VERSION     DAZUMT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.095"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..290)
                     /locus_tag="ACG8FM_002267"
     CDS             complement(66..290)
                     /locus_tag="ACG8FM_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS5094913.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(488..2068)
                     /locus_tag="ACG8FM_002268"
     CDS             complement(488..2068)
                     /locus_tag="ACG8FM_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS5094914.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2469..3845)
                     /locus_tag="ACG8FM_002269"
     CDS             complement(2469..3845)
                     /locus_tag="ACG8FM_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5094915.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3973..5061)
                     /locus_tag="ACG8FM_002270"
     CDS             complement(3973..5061)
                     /locus_tag="ACG8FM_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5094916.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5233..5667
                     /locus_tag="ACG8FM_002271"
     CDS             5233..5667
                     /locus_tag="ACG8FM_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5094917.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5745..6278)
                     /locus_tag="ACG8FM_002272"
     CDS             complement(5745..6278)
                     /locus_tag="ACG8FM_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS5094918.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACG8FM_002273"
     CDS             complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACG8FM_002273"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS5094919.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACG8FM_002274"
     CDS             complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACG8FM_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS5094920.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8447..9829
                     /gene="rlmD"
                     /locus_tag="ACG8FM_002275"
     CDS             8447..9829
                     /gene="rlmD"
                     /locus_tag="ACG8FM_002275"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5094921.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10003..10581)
                     /locus_tag="ACG8FM_002276"
     CDS             complement(10003..10581)
                     /locus_tag="ACG8FM_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS5094922.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10903..11373
                     /locus_tag="ACG8FM_002277"
     CDS             10903..11373
                     /locus_tag="ACG8FM_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS5094923.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         3364 a         2293 c         1949 g         3826 t
ORIGIN      
        1 aaatttttgt gaattccagc ttatttcttg gagttaaacg cttctgtctc agcctctttt
       61 tctttctagc tttttgattt ttctcttttc ttttgtactt ttctcttttc attttcagtt
      121 ctacgcgtgt ctttattttt aactttttcc tctttatgga tgattgatcg tactttttgt
      181 gcaatttgct cgtttttgtc ccagtctttt acatctaata atcgacataa tcgtaacatc
      241 tcttcttttt tctgtttttt tctttcacgt ctttgccatt ctgtttccat gtgtcacact
      301 ctcctttctt taatcatatg tacgcaaaat aatttttata tttttttgat tagacatttc
      361 tatgatttta atatagtaaa aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa
      421 tatgagaggc tgtttttgtt tggctattct tattacttga tgcagggcag cttgtgtcaa
      481 gacttcttta catcaaactt tttaatttta cattgggata tttatccgca aaccagcgtt
      541 ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa acggtcacga gccaagatat
      601 tgcgacttga actcatcttc tcatccaaat cttctggatc gatccagcga gcgattttgt
      661 gtcccattgg agacatgatt acttcggcgt tatactcatt taacatacga tattggaata
      721 cttcgaattg tagctgacca acagctccaa tgatatattc ttccgttaga tatgttttat
      781 aaagctgaat cgctccttct tgaactaatt gatagattcc tttgtggaag gatttttgtt
      841 tcatgacatt ttttgcagtc actttcataa acagctctgg agtaaatgaa gggagttctt
      901 cataagcaac ttttagtttt ccttcgaaca atgtatcccc aatctgataa ttgcctgtat
      961 catatacacc gatgatatcg cctgctactg ctttttcgac gttttctctc gaatctgcca
     1021 taaattgtgt gacattgctt aatttcagtt ttttatttgt acgttccaac cagacatcca
     1081 ttcctcgttc gaatgttcca gaacagattc ggacaaaagc gattcggtca cgatgggctg
     1141 gattcatatt tgcttggatc ttgaatacga aacctgaaaa ttcttcctca taaggactta
     1201 ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg tgccaattca acgaaagtct
     1261 ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc aaaaaatact ggtgtctgat
     1321 cgccacgagc aattttttct tgattaaatg catctccagc ctctttgatt agttctactt
     1381 cttctaagac ttgttcatat aaggaatttt cagttagagg atgattttcg ggaagcagtc
     1441 catcttcatc caattctact aatcgttcat cctgataatt ttccggacga taaaactcta
     1501 cacgattatg gtagatatca taaagacctt ctagcccttt acccataccg attggccaat
     1561 tcatcggata agattcgata tctaatagct cttctaattc ttctaacaat tccaatggtt
     1621 cacgaccatc acgatctagt ttgttgataa aagtaaatat cgggatgcca cgttttttta
     1681 caacttggaa taatttcttt gtctgtgctt cgatcccttt tgcactatcg attaccatga
     1741 ccgcactatc cacggccatc aaagtacgat aagtatcttc agaaaaatct tcatgtcctg
     1801 gtgtatccaa gatatttact cgtttccctt gataatcaaa ctgcataaca gaactagtaa
     1861 cagaaatccc acgttgtttc tcgatttcca tccagtctga tttggcaaaa ttacctgttt
     1921 ttttgccttt tacggtcccg gcttgacgga tagccccacc aaacaatagt aattgttctg
     1981 taatcgttgt tttcccagca tccggatggg aaatgatcgc aaaagttctg cgattgtcca
     2041 cttgttgctt taattttgga ttattcataa cctaactcca tttcttccat tcaattattt
     2101 atattttctc taggcacaga ttgtccgatc tcttaaagaa agacaatttc atatagggct
     2161 gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac ggcgttctag agacagccta
     2221 tcagcaataa gcccacccaa gccaaaaata cgaaaagctg tttttgcttg gctgctctta
     2281 ttacttaatg caggatagct ttttcacaac ctttgtcaac tactacaact gaatcagtat
     2341 agcatggatt cagcgaatga aaaagccacc aaaccataag agtcaaatat ttttttgtca
     2401 gctgactaaa agaaaagacg tgttgcttgt ttattcaagc aacacgtctt tcataaccaa
     2461 gtttccaatt atcttcttgg ctcgtcatct tcgaattctt ttctgaagta tcttcgttct
     2521 tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat tttaaggatt
     2581 cgagaacctt ccatttcttc agaaaccagc gtaatattat cgacatcaaa cgataacttt
     2641 tcgccttcat ccggaatcat gcctaatgct gtaatcaaat aaccagccat cgtatctaca
     2701 tcactcatat gcagatcact gtcaaaagct tcattgaact catcaatcag catacgtcct
     2761 tggatgatat attcgtgttc atcgattttt tcataaagat tctcaacttc atctgattca
     2821 tcatcgattt ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg
     2881 ccgccatatt catctaataa aatagccatc tgattctgcg tctttttcat ttcatataac
     2941 agatcatcaa taaagaccgt ttcaggaacg aataaaggct cctgcatgat tttcttgata
     3001 tctaaattat caaagccgaa tttatgggca gcttttagta aattctttgt atgaaggatt
     3061 ccgactactt tatctttgtc ttcgttataa acagggattc ttgaatagtt ttcggataaa
     3121 acttcattca cattttcttc cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt
     3181 gggaccatga cttctcgggc aactttcgta tcaagagaaa aaaccccttg gagcatttct
     3241 aattcttcat tattcaatac gccttcgttt tcaagcatat aacgcatttc atctctggtc
     3301 atttttgaat cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa
     3361 gcagaaagca gccagacgaa gggcttagct actacaccaa tcaagcgtat cagccctgaa
     3421 gtgaactttg cgacttcttc tgatttattt aaagcaatac gtttagggta caattctcca
     3481 aaaacaatcg atacataggt caaaatagcc agcacaatga tatttgcaat attttttgcc
     3541 gctgtgccgc cacctaatac cggagctaac cgttccgata aagtattagc tagtgaagca
     3601 ccagacaaaa tgttgaccaa agtgattcct acttggattg tcgataggaa attatttgga
     3661 ttctgcaata tttttaaaag cttttgagat ttcacatctc cttcatcagc tttttgctcg
     3721 attcgattct tattgatcga aacaacagca atttcggaag ctgctaaaaa agcattgatc
     3781 aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg actgactctc agggtcagca
     3841 ttcataatcg cattctctcc ttataaaatt aatttttagt ttacgataga aaaaaacgtg
     3901 gcactatagc cacgttaaaa tggtatcaca aagcaatttt ctttgcaata aaaacaagtg
     3961 tactaaaact acctacgagg tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc
     4021 tttgacgatt ttgattatat aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc
     4081 taagaagata ccaagcaatc cggcaatatt tccagctact agaagaacga taatgatcgt
     4141 taatggatgg attttcagtg atttcccaat tacattagga taaatgatat tcccatccaa
     4201 ctgttggacg attagcacaa ccacacaaca aagtaatgct tttaccggat cattgaagac
     4261 tgttactaga aatgcaggtg ctagtcctag ataaggtcct aaatatggaa ttaggtttgt
     4321 caatcccgca atgaccccaa ataagaaagc ataatccaca ccaattacca tatatcctaa
     4381 aaatgtgaag gtaccaacga ataaacattc gattgcttgt ccgctaatgt aattagccaa
     4441 tgttttattc agttgcccca ataactccac aatttggttt cttctttttt ctgggaaaaa
     4501 ctgttggatg tttggcacta gtttttctcc gtctttcaac atatagaaca agatgaatgg
     4561 tgctgtaatg attactacgg ttgccgaagc gatcgttcca acgatcgaac ctaaactatt
     4621 ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa
     4681 atatttattg atatctactt ccttgaacaa aggcaattcg gctacctctt ttaaccagct
     4741 ttctacctgt ttgatgaaat caggcatatt agatgccaat gaggaaattt gctgtactag
     4801 acttgggatg acactaagta aaatccaaat aattgctgca actaaaagga gcagcacaat
     4861 tgcgacagcc catattcttt tgatttttgc tttcatcaac agcttgacaa taggattcaa
     4921 taaatagtaa agaaagcctg caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc
     4981 gatcggttcg aaaataaagt ttatttctga tgaaatcatg atcaatgcag ctatgatcaa
     5041 taattcaagc gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca atagatttcc
     5101 tcctttcttt cttactagta tatattatat tcgttaaaaa ttcttttatt ctttttttat
     5161 ttttgaaaac ttgccatact ttttatttgt tttattttcc agtatactat acgaaaggaa
     5221 ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat ttacttagat
     5281 gctgtacgga tcaggcatca agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa
     5341 atagacaaag atgaagccca ttgtatccac ttcgtcctgt actcagataa aaaagaaccg
     5401 caaggaactg tacgtctttt gcctttagaa aacggaaaaa tgaaattaca gcggatggcg
     5461 attctttctg aatacagaca ccaagggtta ggaaaaatat tgattgaaga agcagaaaac
     5521 tttgctaaga atcaaggcta taatacgatt ttattagggg cacagtccac agctgaaact
     5581 ttttacgaaa aactaggata tacagcttac ggcgatcctt ttgaagatgc tggcatgcct
     5641 catatttata tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag
     5701 cttttgagaa aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat
     5761 tgcttgtaac ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta
     5821 ttgatccagt caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcggg
     5881 taattcatca ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg
     5941 tcaggaaata ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc
     6001 aaaaaaggtg aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt
     6061 acattgaacc agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg
     6121 cgtccatcag attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt
     6181 gtttttaata ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt
     6241 tggatcgtca caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca
     6301 ttttgtgctt tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa
     6361 atgagctttg cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt
     6421 ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa
     6481 gttttgttct cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga
     6541 acatgccatt tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa
     6601 acaaagagtt cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca
     6661 aacagatcga tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt
     6721 gtcacttcca tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct
     6781 tcaaaataat acgctccaga atgattttga agagctgcac tgatataata aacgtctttt
     6841 ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa
     6901 caaaaggctt ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa
     6961 cctagatcca tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc
     7021 atcgcttcat caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac
     7081 aatctgctga ctactcgcat cacgttgccg tcaactgcag gttctggaag tccaaatgcg
     7141 atacttgcaa tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca
     7201 ggcgtttgcg gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg
     7261 ttccgggctc ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga
     7321 gcatttgcta gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc
     7381 actgtgtcta ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca
     7441 cgattatagc gccaaggcag gtttcttttt tcttgttcat accattgaat gaactgatct
     7501 tgaaattctt ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc
     7561 cttttccgta atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg
     7621 ctttactttt agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc
     7681 tccttgtttg actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc
     7741 tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc atcaatgttt gttggatttt
     7801 tctcaataat tctttttggc tttttccgtg attttttcca aacgcttttt cggctgtctg
     7861 atgtgcattt tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc
     7921 aggtttcaaa cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa
     7981 gcgcatattc gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc
     8041 ttttaagcgg gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta
     8101 tgttcggatt tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa
     8161 accgaaatca gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg
     8221 tccttttaat aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc
     8281 agataaatct acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat
     8341 aaacatcctc ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta
     8401 ttatatgtta gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat
     8461 tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg
     8521 aatcggttat ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt
     8581 actagtgaaa atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa
     8641 aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg
     8701 tcaattacaa cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca
     8761 agcattggaa aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg
     8821 gatggatgag ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca
     8881 gactcaaaaa attgaagctg gtttatacga agcaaactct catcagttag tccctataac
     8941 agactgcttg gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt
     9001 gaataaattt gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat
     9061 catggttaga atcggtgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa
     9121 aaaattcccg caaaaaaacg caatgatccg tgagatcaat gaacgactgc ccgaagttgt
     9181 ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat
     9241 ccacttatgg ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc
     9301 tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat
     9361 caaagcatta gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac
     9421 gatcggctta agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca
     9481 agcaatcgct gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga
     9541 agtcgggaca gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg
     9601 tatcatcgta gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa
     9661 ataccctcca aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct
     9721 ggttaattta agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca
     9781 gactgcacgt tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt
     9841 tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat
     9901 gttttatgaa tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa
     9961 aaagggttgt gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc
    10021 tatcacttga taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt
    10081 atatcctttt cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt
    10141 tgctttgtcg tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg
    10201 attccaagtt ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa
    10261 attccaagta atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt
    10321 ctgttcatct aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa
    10381 tgtatttttg gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt
    10441 taagtttcct gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc
    10501 atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa
    10561 taacgtacta atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc
    10621 tttattgtat taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga
    10681 aaaaagtaag atgcagatta taacagaagt agacgagaaa agtacaaata gaaaacatga
    10741 gaatcaaatc atatgatctt tttcaaaaac atcattcata taaacaaatg ttcaactttt
    10801 taataacaac aattatcaat ttttttctta cttcacaaac taaatacagg tcttgctgtg
    10861 agccttcatg gtaaactaat attatagaaa ggagttagta aaatgaagat gataaaagat
    10921 attcgttttt gggtttgtgt tataggaatt gtcatattag gctttttatc agggttgctt
    10981 agcggaaatc ccggaaaata ttactattct ttgcaacttc caccatttgc accacctagc
    11041 tggatatttg ggccgatgtg gaccttactg tatatattga tgggtatttc tctttatttg
    11101 ttacttaatc acaacaacaa aaaacagcga aacaatcttg tcggtctttt tgtgattcag
    11161 ttcattttta attttatatg gtctgctcta ttttttaatt taagaaatat tttcattgca
    11221 gcaatcgata ttacactttt agttattttc ttgtcagtgc ttatgtatca gttgtggctg
    11281 catcatcgcc tggcaatgtg gctcatgatt ccttattatt tatgggtact attcgcaact
    11341 ctattgaatt attcaattta ttttttaaac taagcgctaa aaagagacgt cctgtaattt
    11401 aacacataga aaaagtgcca cctaagttag at
//
LOCUS       DAZUMT010000095        11411 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000095 DAZUMT010000000
VERSION     DAZUMT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.096"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..1492)
                     /gene="tyrP"
                     /locus_tag="ACG8FM_002278"
     CDS             complement(71..1492)
                     /gene="tyrP"
                     /locus_tag="ACG8FM_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS5094924.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="ACG8FM_002279"
     CDS             complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="ACG8FM_002279"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5094925.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3903..5159)
                     /gene="tyrS"
                     /locus_tag="ACG8FM_002280"
     CDS             complement(3903..5159)
                     /gene="tyrS"
                     /locus_tag="ACG8FM_002280"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS5094926.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5271..5476)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5739..7040
                     /locus_tag="ACG8FM_002281"
     CDS             5739..7040
                     /locus_tag="ACG8FM_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5094927.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7109..7708)
                     /locus_tag="ACG8FM_002282"
     CDS             complement(7109..7708)
                     /locus_tag="ACG8FM_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5094928.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7963..8844
                     /locus_tag="ACG8FM_002283"
     CDS             7963..8844
                     /locus_tag="ACG8FM_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5094929.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8857..10467
                     /locus_tag="ACG8FM_002284"
     CDS             8857..10467
                     /locus_tag="ACG8FM_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5094930.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10544..11203)
                     /locus_tag="ACG8FM_002285"
     CDS             complement(10544..11203)
                     /locus_tag="ACG8FM_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5094931.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT         3326 a         2281 c         2075 g         3729 t
ORIGIN      
        1 aaaaaactat tcacttggca ggacctccta ctaaatcagc gacaaaacgt cggctgaaag
       61 acttattttt ttatttttct tctaaacttt tttctacctc aatcccccaa gaatctttct
      121 tgaagtgatt gatgatcaat ggaataacga acatgaccac tgtgatagct gctactaata
      181 atgcatcact caatgtcgct gtacctaatg tagcaaatac gacaacgatg atagagaaga
      241 tcagcatgca gctgactacc caagctaatc cgtttccgtt tttaccaatt cggtaaggac
      301 gttcgaattc tggatgtttc ttacgcattt taagcaatga aacagcaatc aaaatgtaca
      361 cgatacaata aataacagtt gtagcatttg taagtgtcaa gaatacagca cttacgtcat
      421 ccattacacc ataaagcaaa gcaaataatg aaataacgat tgattgtgtt agaacgacat
      481 ttctagatac gccgtatttg ttttctttat ggaatccgaa ttttggtgga aggaatcctt
      541 cacgagctac ttggatgatt gtttttgatg gtccagtaac ccaagctgat aattgtaaaa
      601 gcacaccaat aaataccatg aagctgaaga tatttccgat cacttctggt aagcctagga
      661 tttgacagtc aatcaagata ggctgcgtga tatttgctaa ttccattttt ccatctggta
      721 cgacatttgc tacaagcatc gcattgatga cattcaataa aactaatccg atcaaagcga
      781 taaatacgcc ttttgtataa ttttttgttg cgtctttcaa acgaggcatg taaacagaac
      841 tcatttcgat cccaacaaaa ataaatgtga taccagctaa gtatttcaat gaatcaatat
      901 gttccaagtc agggaaagct tttgaccatg aaaagtcacc tagatagccg tttgagacta
      961 gtccgacttt gaaggctgcc aaaacaccta aaacgaacat gatgactact gggatatata
     1021 cccctagcca tacaccgatg ttcccgccga cttttgccat atcgaatttc aagttcaaga
     1081 tcgtgatgat ccagtagata actaaaatac aacctaaaac gaaccagtga ttattgccaa
     1141 gttctacatt accaaatgtg tttcctaaca atggtcctaa agtagaagcg accattacca
     1201 tacctgggaa catttgcacc cataatagcc atgcaacgac aaatccccat ttgctgccca
     1261 gtgctgtttt tacccataac tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac
     1321 cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca
     1381 atgtccaacc gactgcggct aacgtaggaa tactccgaac cgttgcacac agtgccattg
     1441 tcagaccgat aaaggtccca agacccattt tacttacttc agatttactc accgttgaca
     1501 ctctccttga ttttttggat aaaataacgg tgtctttgaa gtagtttcag caataaacga
     1561 aacaattcag cgaatatttg aggggattct ttgagtttat tgcttgaagg gtttcttcaa
     1621 agacaacctc aaaacaaagg gatcgaagag cttaggtctc cccttgctct tcagatccaa
     1681 acatgcgtca gaaacagtta gtattcaagc tgttttctaa catgattacc ttgcttattt
     1741 tgcttcgctt gccaataatt ggtctgcgta gattttttcc aatttttctt gaagagctgc
     1801 tttgattttt tcagcatatt cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac
     1861 gctggcacgc aatacagtta cttttccggc acggttccat tcttcatctg agaatcctag
     1921 ttggttcaca aattgtaatg ggctgttgcc atagtctggg atagcaaagt cagtatgtga
     1981 agttaagaac tcattaccgt aaatgcttcc tttcacgtat gaagaataat catatacgtc
     2041 atggttcaat ttattcattg caactaagtc atcgttgcct ttttctttga atacatagtc
     2101 taccatattg aaatctggat aagttaatgg atgaacttcg atttctttgt ctcctacttt
     2161 aaatgacaag tcattcaaga agttgtagaa acgatgtgca ccttcgattg atgcacccat
     2221 taatttacca tatcctgtga cattcaaagg taagacatgg tgtgctgccc aaacgctggc
     2281 tgctgttgcc cctgcttttg aaccttccaa gatataagcc ccaagtaatg ctgggatatc
     2341 tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata acgtctctca tgcggatatc
     2401 tttgatgacg ataccaccgg cagaatatgg aacatagccc attttatgtg ggtcaatcgt
     2461 tacagattct gcttcttcga ttgctttata tgcactgtgt acttcttcta agatataatc
     2521 tttgttttct gtaaagacat tgtatttgta atgaacatct tttaaatctt caaatggaat
     2581 gaagttgttg tcttcgtcta agaaaatcgc acggccgtat ccgccataag cagcatctac
     2641 gtgtaagtag aagtaaatac cgtctttttc taatacgcga cgcaatgcaa cgattttatc
     2701 gataccgtcg attgctcctt cttctgttga tcctacaaca ccgactacac ctaggattgg
     2761 tgttttttca gctgctagac cacgaacgat tttttcaagt tcgttgatgt ccatgcgata
     2821 gttgtggtca actggcactg gaatcacttg gtctaaaccg ataccgatga tgtcggcagc
     2881 ttttagccat gaatagtgtt tagtttgtgg aaccaaccat tttcctaatt tttctaaatg
     2941 ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt ttttctggga cgctgtctaa
     3001 cagattcatg atttcttctg ttgacaagtt cattaattcc caatcgcttt ttccagcaac
     3061 tagttcaggt gtcacttctt tcattgcaag cggcaatgat ttgatgttgc gggcatacca
     3121 aagtccttct aagtttgcta aagaaccatc agcaacgatg tggccccagc catctttata
     3181 gctcattaat tttgcaaatt ccattcctac ttcttcttcc atttggcttg ttgcaggaga
     3241 agattcatag gcaacgttat taccattcca tagcatagca aaattgtacg ctagtaaaga
     3301 tggcatcaat gtttcagagt tcatatgacc ccagtaacgt ccggcattat gccaaggaac
     3361 ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca tcctttgttt tattcacagt
     3421 gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata ataggcatgt cttgaggcat
     3481 gtagttttga cgccagccta aatgttcatc caccaattca ttcaacaaag ctttatagat
     3541 ttggccgttc tcagctttgt ccccaatgaa tagggcattt aggtttagat ctttcgacaa
     3601 tgattcactc atagtgaaac cctcctaaaa aaatatttaa attttgtcga gccctacatt
     3661 acacctgttc ttctattctg taaactaagt tttggcctga tttttaatca tattttaata
     3721 tgtatcaatt atccgaattg ttcacacttt tgaaataatt aaaaaaaacg agggaattta
     3781 caaatgtaaa ctccctcgca aagattgtat taacgacaac cagcgttcta ttttaggatg
     3841 ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt caaggtaaaa acttactaaa
     3901 ttctaatcaa ttactttggc caaaaagtaa tttttcttac cttttcgaat aaccacaaac
     3961 ttaccatcga attcatcgga tggatttatg acaaaattta aatcggtaac gcggtcaccg
     4021 ttaatactta tagctccgtt tgaaacatct tcacgtgctt gacgtttaga tggttcaatt
     4081 tttgtggaaa caagcagttc cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc
     4141 attttaccga atccttgcgc gatttcttcc gcatttagtt ctttaatatt tccagagaac
     4201 aatgcttgcg ttattttttg cgcttctttc aagtcttctt cactatgcac aaatcttgta
     4261 acttcttctg ccaaacggcg ttgtgcttca cgtttctcag gttctgtctc tacttttttc
     4321 gctaagtctt cgatttcttc ttgagacaag aaagtaaaga atttcaagta tttcactaca
     4381 tcgcgatcat cttgattcaa ccagaattgg tagaactcaa atggtgaagt tttcttcgga
     4441 tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc
     4501 aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt ctaattttcg gattaggtct
     4561 aatccagctg tgatatttcc ccattggtct gctccaccga tttgcaattg cacatcgtat
     4621 gttttatgca gatgcaaaaa gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat
     4681 gagattccta cttccaaacg gctggcaacg atatcttttg ctaacatcgt gttgatgttg
     4741 aagtttttac cgtagtctct caaaaattca agtaaagaga tttttgacaa ccagtcgtag
     4801 ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt ttctcatttg attggaaagt
     4861 gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca cacgttctgt tttgcggccg
     4921 cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa tatatggatg atggcctgcc
     4981 aattggaatc ttttcatcat cataaatgga atcaaatgtc cgatatgcat cgaatcacca
     5041 gttggatcaa cgccgcaata aagtgaaatg ctcttttctt caacgagctc gcgaagtctt
     5101 tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta attcatcaat aatattcatt
     5161 ttactgacct ccgtgtagtt tttttatgaa agcaggaacc tgctagcatg tgtaattaat
     5221 tatttcttca ggataggata ggaaataaaa aaagtccctt ggcatataag ccaagggacg
     5281 aaatctcgcg gtaccaccca atttgtatgt ctagatatcc aacatacctc tcgaacattt
     5341 aacgctgtcg cgtggcaatc actgccaaag actcctgaaa tgtaattcgc tttaaagcat
     5401 gtactggttt gcagcgacca ccagctttct gaaacattgg ctttaacaac tacttcgttt
     5461 cgatcttcgt cttgttatta ttaatatgtg tctatcatag agcgcaactt tttctttgtc
     5521 aacagaaaaa ggatattttt tttaaattcc tcattttttc ttttttgaat ccgctctaat
     5581 tccgaacatt ttattttcaa ttattgttaa aagaaataac tttttctgta ttttttctgc
     5641 aatttatcga gaagtcactt tactttgcct cttattcttt cttcgttaca ataaggaatg
     5701 ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta
     5761 ttttaggagc cacttctgct ttaactatcg ttattttaat tctaagtttg ttttttgtca
     5821 tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa
     5881 aagaaccgac agatgataag caacaactag ttactcaagc aaaaaatctg gctcaaagct
     5941 acgattatga ccaagcgatt gcgttattgg aaaaagatag taagaacgga tctactgaaa
     6001 cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca actgatcgac tggtctgatc
     6061 ctactcagat ctctcatgtc tttttccaca gcttgatcgt ggacccgcaa aaagccttcc
     6121 actctcaaca ggcacaagga tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt
     6181 cgatcgaaca actttatcaa aatgattttg ttttagttaa tttgaatgat ttgatccaaa
     6241 aagacgaaca aggaaatctt tcctttaacg ggcttgcgct acctgaagga aaaaaaccat
     6301 tggttctttc acaagatgat gtcagctatt atgaatatat ggataattcc ggttttccta
     6361 gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt ctataaagag aaagataaag
     6421 aagaaatcgg tgactttgac atggttcctc tgatcgatgc ctttgtcaag aaacatcctg
     6481 atttttctta tcgaggcgca aaaggtacct tggccctgac aggttacaac ggagttttag
     6541 gttatcgaac atcaaaatct caatatggag acaatgaaaa aacaaaaaaa gagattaaag
     6601 aagctaaaaa agtggcggaa cagttgaaga aagacggctg gagctttgct tcccatacat
     6661 ggggtcactt gaatatgact caagcttcac tagaagatgt aaaaaaagac aatgaattgt
     6721 ggcagcaaga agttgcacct atcctaggaa aaacaaatat tctgatttat ccatttggtg
     6781 cagacatcag cgactggcag ccttattcag cagaaaatca aaaatttgct tacttgaaga
     6841 gtcaaggttt cgatattttc tgtaatgtcg atgcttctac accagcttgg ggacaattag
     6901 gtacggattt ctatcgaaat gctcggatca atatcgatgg cattcgtttt caatctgatt
     6961 tagacggaaa aaatcctatt cttgatccat tcatcaatgt ccatgatgtc tatgacaaag
     7021 aagcacgcga atctgcttga ttttacgtca tttatgaacc aaaaaagccg aactgacatt
     7081 ttatcgtcag tttcggcttt tttatgcttt atttcttctt cggtgcgatt cccttgatca
     7141 ggacttctgt ccattctgct gtatatggaa gcagttttgc gtatatctcc ggcttagaaa
     7201 ttccttctac cattgcttga aaatagagga tcaagaactc atccgaatat ttcaaatcca
     7261 cttttccttc tttccgccct cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg
     7321 tatacgcatc catcgcgtca gaaaattctt ttttttcttt ttttgtatag tatgtcatca
     7381 aatcgatata aaattgtttg ttcactcgat tgagataacg gattttgttt tcactcatgg
     7441 caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta
     7501 aatcattcat aaatgcaaaa aatacttcat aaatcagccc ttccttgctg ccaaaatatt
     7561 taaaaatcgt cgctttactt acctgagcgt tctcagctac cttatccatt gtcactccag
     7621 cggttcctat gtctgtgttc atcaagtcaa aagctgcttg taaaatctct ttctttttct
     7681 cttgtgctct cttttcaaaa ccgttcattt gtagagcctc ccgtaatttt aaacttttat
     7741 aatatatata gttcaaattt attctgaggt ttattataac tatctattga gaaagtatca
     7801 agctagcaat tccttgttta tgaattcatt gacagaaatt tataaaaaga atattatgta
     7861 ctaaaataat aaaaaaaagt tttataaaaa ggtataaaac ctttttatta aactatttaa
     7921 tcttgtttag tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa
     7981 gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc
     8041 gtacattcag gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg
     8101 attcggacac ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa
     8161 gatgtatggg aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc
     8221 tctttgtggg gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga
     8281 aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa
     8341 aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct
     8401 gttgattcgg atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa
     8461 acgattttcc aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct
     8521 tctcatattc tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga
     8581 acactcgatg aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact
     8641 atagaaacag aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg
     8701 caaaaaggtc acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct
     8761 gaagcgtcca aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta
     8821 ttcatgagac attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg
     8881 tccacccttt tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc
     8941 ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa
     9001 ggattagctg gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca
     9061 actccagctt cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg
     9121 cttttttgca gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga
     9181 aaaaaagaag aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca
     9241 aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc
     9301 agctgtacga tgattttttt ccaagcggat tcgatcactg cagaaggtgc ttggcttttt
     9361 gggctatctg taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa
     9421 ctgatgccaa gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac
     9481 cttctacgag caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc
     9541 tggtcttatt tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt
     9601 ttgttcagtc taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac
     9661 accggctata tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg
     9721 cgtggtttat tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt
     9781 ttgatggggg cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac
     9841 gacttaatga aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc
     9901 acgatcatgg tagtcttgat ttgtctagct gctattttac caattgctag catcaatcgt
     9961 ttatttacag aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg
    10021 aaccaacttt attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg
    10081 attagttctg gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg
    10141 gatctattga tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc
    10201 ggaattagtg gctttctatt cggatggctt cctcgaatcg gtaaacttgc ttatatctac
    10261 ttagcttact cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt
    10321 tccaaaacag ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc
    10381 gtgttcctta cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat
    10441 aggcgaagag acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc
    10501 aaatctgggt caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact
    10561 gctctttatc aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta
    10621 ataatttaaa ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt
    10681 tcattttcat tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat
    10741 agacagcgac tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt
    10801 ttgttaattt ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta
    10861 agagttccga gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc
    10921 caaataaaga ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct
    10981 ctattactcg taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc
    11041 cggaggccac gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt
    11101 ccacatagtg atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat
    11161 tgaacaaagg aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta
    11221 aacagctatt tctaataaag aaacataact tcccttattc caactgataa ttttctttta
    11281 tcatagcata aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc
    11341 cttgctttat tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat
    11401 atttactttt t
//
LOCUS       DAZUMT010000096        11160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000096 DAZUMT010000000
VERSION     DAZUMT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.097"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..1041)
                     /locus_tag="ACG8FM_002286"
     CDS             complement(130..1041)
                     /locus_tag="ACG8FM_002286"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5094932.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1418..2056)
                     /locus_tag="ACG8FM_002287"
     CDS             complement(1418..2056)
                     /locus_tag="ACG8FM_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094933.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2060..4042)
                     /locus_tag="ACG8FM_002288"
                     /pseudo
     CDS             complement(2060..4042)
                     /locus_tag="ACG8FM_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4222..4887
                     /locus_tag="ACG8FM_002289"
     CDS             4222..4887
                     /locus_tag="ACG8FM_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094934.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5284..7317)
                     /gene="brxL"
                     /locus_tag="ACG8FM_002290"
     CDS             complement(5284..7317)
                     /gene="brxL"
                     /locus_tag="ACG8FM_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFS5094935.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7435..9957)
                     /gene="pglZ"
                     /locus_tag="ACG8FM_002291"
     CDS             complement(7435..9957)
                     /gene="pglZ"
                     /locus_tag="ACG8FM_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFS5094936.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10073..>11160)
                     /locus_tag="ACG8FM_002292"
     CDS             complement(10073..>11160)
                     /locus_tag="ACG8FM_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS5094937.1"
                     /translation="LPFLKVTSERKLKKIREKVDENIDIARKDYNALETSYEFRRNIL
                     LSYMFKKDLSEAFNCLSRQLVNQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYIS
                     ARLPDKAREIKDFISYSIGCIFGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLIT
                     DDEYFKDSQIDIVNRFIDFVEITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDF
                     YKDHLKTYSNRPIYWQLDSGKQNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRI
                     ELRQQQISTSDNPKERNAMTKEIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIV
                     NYDKLQRDPKTGEKFQIFAKGVKEPKK"
BASE COUNT         3543 a         2139 c         1614 g         3864 t
ORIGIN      
        1 aaaaaaattt ttaaaataaa aagaactaaa tcaagtaata attaaactac tgttgattta
       61 gttctaataa aaagatttag atgaacagtt ttattgattt aaatagttca tattttttat
      121 ttataccgct tatttactac ttgctgtact agaatctgaa gtgaatgtac tcaaaacatt
      181 ttcaaagtct ttgtctttga tttttacatt agcgtctttc aatacttcgc caattacttt
      241 attctggaag gtactgtcac ttaattttgt gtctgttgca atttctttca attctttttt
      301 gtatttatcc atgtcgttgc ctttgttttg gtttttcacc attttaacca cataatattc
      361 cgtagtataa gttgaagcat tggtagaagt gatcacatca gaaacttgac catctttcaa
      421 cttaaaggct gcttctttga cttctgccgg aaccgttgta gtagtggaat caaatttcac
      481 ttttccgcca tcttcttttg tcgtagtatc agttgacttg tctttggcta atttagagaa
      541 atcgtctccc ttatcagcag ctttcttcac atcttttgca tcatcttcac ttgctacttg
      601 gataatttga gcttccactt ctggatggaa agattcccaa gccgttttta aatcttcatc
      661 ggtcaatttg atatgtttct ttagaccttc ttgaaaggct agactttgtt tgatactgtc
      721 tttgaatgtt tgttctgtgt aacctgcaga tttcaattgt gaatcaaatg aatcacccaa
      781 ttgtttcttt gtttgatcat attgggaatc gatttctttg gttgatacgt catctccata
      841 tttatcttca aatacttgat aaacgatcaa ctgttgcaca gcggaatctg ctccttgcat
      901 ttttttcaag ctatccatta cttcttgttc gctgattgta ccgcctttat aactaacggc
      961 tgttttttca ccggcattcg tacagcctga caataagcca gtgcctagta cgatggcaag
     1021 tgctaaaaat ctttttttca tttatcaatt ctcctacttt tcgttttttg cgaattgcta
     1081 aaaacaaagg tgttcaagat gaaaatcatc aagaacacct ttactaatct gctcgatacg
     1141 tcctgaatag atataaggct acacaatcca gaaagttcgc tcagcagaat aaatgtcaag
     1201 tagcacatca caccttaata tactacttct tttgttaccg tttttcaagt ctatcttgcg
     1261 ttccgtattt aacgtaacta attcttagaa caaatgttat cgattttgtt tttcttcttc
     1321 cgctgttggg atattcaaat cagacacttt tatgttcgat tttccagaca tttttttgtt
     1381 gtttttatta gaaaaagttt gttttttcat ttgttttcta aagcatgatc ttcttgtttc
     1441 ctattttggt cagatatgga tcatgcgtga ccacgataac taacgtacct tgggaagcct
     1501 gttgcgataa gatttcaacg acttgctcct tatttcgttt atctaatgat ccagttggtt
     1561 catcggctaa aatgattttg ctattcttca ataagactct cgcacaggcg attctttgct
     1621 tctcaccacc agataatgtg tagataggcg tacttagctt cttgtttata tttacttttt
     1681 tcaatgcctc atactttgct gttcgcttat ctgaaacatt taaattcgaa aacttcaatc
     1741 caatatctaa attaaaatcg attgtttcat attcaatcaa cccaaaatct tgaaaaagaa
     1801 aagagagggc atttcttttt atcaagcgct tctctcttcc agttaaatgt tttgtttctt
     1861 gattaaataa ataaaattgc cctgaactga attgatcatt caatcctaat atattcaata
     1921 aagtactttt ccctgctccg ctctctccaa ctacagtcac aaaatcccca gcatccgcag
     1981 tgaaacgatc caaatgaaga atcgttttgt ttcgaatgat ttttttcgca ccttttatca
     2041 ctatcaacgg ctccaattgt taacctccat tcatcagcaa taactgactt ttcttttctg
     2101 tagacaaaat aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa aaaatattaa
     2161 aaagatattt aggctctctt tctataagaa aaaggaagat tacacttgtc gttccccatt
     2221 tgatgaggtt catcatgaag aaatctttat acgtttcaaa gagcgaataa ccacttgaac
     2281 gaaagatagc gattttccga caattgtaga caaaatatat gcataacatg acatagctca
     2341 acaagaagaa agaaaagaaa gtgagtaaaa atatctgtat caagtaatga aatgatctgc
     2401 ttaacacttt gacagctatc tcttcagtct gagtatacgg aaatgataac tgcaagttat
     2461 cacgtaactg actttcttca atcagctcaa ataacctttc tttcgacaaa gagcctatct
     2521 tgatctttaa cggtggatag atttctccgc tgagaatatt tctttcccaa tacgtactgt
     2581 ttttcaatgt cagtatttca acaaccggat aatgttcgat ccaggggata gaaggaataa
     2641 atgtataaat gggttgattg ttttttataa atagaaattt cggttctttt tcactgattt
     2701 cttgataata ttctgttatt tgcgccaggt cattttttaa atctgtatgt ctatatcgtt
     2761 cagggatcaa tataacaggg tgcgtttctt tttcggttat ttggatcaac cttccagaga
     2821 catcaatcaa aggaaatttc ttaagataat tcagatttat ttttatccct ctgccaaact
     2881 gttcattttc ttcattcaag taatctgata cattgactaa tatacttccg tttttattca
     2941 aagtttcata taattcctga tcggctgctg atgcaaaacg attggaataa ataaaatcca
     3001 cgtggttttt cccgatggtt actggataaa atacatgata gtcctcacca gcgatttctt
     3061 ttggtgtttc atgggaaaca gctaactgtg aagaagaaaa aacgatagtt gctaattcga
     3121 cattggcaac ggtcaaaaga agagaaaaca acaatccaaa tgtaaaaaga atgtattgga
     3181 tatagttgaa cgcttctctt aacgaagcga ttttttcaac taataaaaga gaaatcaaag
     3241 agatacccta tattccgctg aacaagaaaa cgtacatctg gaacaaataa agaaattgct
     3301 ccttataata cataaaaata aaaccaccaa tgattcctgc aattatgaat gtaataatga
     3361 ttttcttttt ccccaataaa caccacaata cttgatgtaa tgaaaagcca ttattcaaca
     3421 ataaactgat ttctttattc atcaataaga aaaaggatga taacaaaagg aataaaagaa
     3481 tcaatccaat agttaaatac actcgataat tatatacgtc tgtacttgaa ataaccgaat
     3541 acgaatagct attttcaaag tcttcaaaaa tattagtggc ataatcagtg tgaaatgctt
     3601 gattgaatcg ttccacaaat agtaaaatga accgttcata ggcttctttt tctctcgtca
     3661 tcaagaaaaa ttcaccttca aaatctttta ttttgatcga ttcttcaatg gacgttacgc
     3721 gaaaggaggt attagaatag ggtaccttct cgtgagaaga tttgacatcc ccttgataat
     3781 acaatgtcat ttcaagcttt ggctgtatct tgaagttcca agatgtatct acttcattgg
     3841 attcatttac tgtttttttc atataagaaa gctcagtttc tgatgagaca ttttcaaata
     3901 ccatgattag ctgctcatat tcttcttgcc caataaaatg ttgtggcaat cgaatcggag
     3961 tataagtatc tgtttgatgg aaaaattcat ttgtccgata gagtacctgt ttatttataa
     4021 taaccaaggc aaaaccactc atgataccgg tcactatgat tgtgagtaaa actgtgaact
     4081 tttttttgat tatcaccact ctcccttact taaatgattc tttgataaaa tcataccgtt
     4141 tttcatcaaa aaaacgtatc tatcaaatgt ataggtcatg tagaataaat ctatatactg
     4201 tagaatcgaa aggagaagaa gatgaacatc ggagaaaaaa tacgaactta ccgcagaaat
     4261 tgtaagctta cgcaaaaaga attaggcgaa aaaatcggtg tttccgataa aacaatctct
     4321 agttgggaaa attctcgtac aatgccagac cttgaaatgc tgtcactttt acatcagact
     4381 ttaggtctgc cgattgattt tccttctatt gaatcagcta ccgaatcaat agaaccaaca
     4441 ttgccggatg aacctcttgt tatcaagaaa tcttattcat caaaacaacc cttctcactc
     4501 aaaaagacac tatctttctt tctgctcgtc ttttgtttgg tctatctttt tcttatcaat
     4561 tatcaatata aaaatgaaac ggttgatcga ttcaatcctt ttttgaaacc acaaatcggc
     4621 tataccacac tccctgtagg atacgtggaa aattccatcg aacattgggt atgtaatgat
     4681 atctttgttc atggtacatg gctgtctttt cacgctggag aggtagaaaa aggtaagaat
     4741 catgctgtaa tcaaacacaa aggaaaacac gtcatgaacg ttcgcctgct cacttgggaa
     4801 gccattccct cagattacca agaaatcctc atccaagact atgacgatgc cttaaaaaga
     4861 agccctcagg agccagatag gcattaagta ggagatgaac gtagagatag aaaaaatcaa
     4921 tgcacatgat cttatcaaaa aaaaacaggg gtacaaatta attgaaaaag ctttgagatg
     4981 aaagttccta aataaaaaag ttcagaatga taggtaaaaa aatgactaac tattctagta
     5041 ttaggctatt tgtattattc ttctgttatt tttatatcaa aaaatccaga aataactaaa
     5101 tataggtatt tctggcttat agtataatat ttacgccaca gcccccaatg attctttttg
     5161 gaatcatgat caaggacgga catacacaaa aatattttgc ccattcgatc cgaatagatt
     5221 aacgactaat ctagtaagta atatagattg gtacgtagtc aaaccagcaa atggaaaagt
     5281 tgtctattcc accccaaggg ctttaaagac agcatcttcc ggcgtttgat aaaaaatcaa
     5341 attaaaggta cccattaatt ctgggggtac agttccaata tcagcagcgc ttatcatagg
     5401 tacaagtact tttttcgctc cggcatcttt tgctacttgt aaacagttag ctaaattatc
     5461 taccttaatc atagaaccac caattgtaat atcccctaaa ataactaaac ttggtaatac
     5521 aggtttattt aaggaaactg agcataaagc tattatagtt gataacgata atgtttttgt
     5581 cataccaatg ccattcatat cttgaacatg aacgacaaaa tcttttgtcg tcaaactaat
     5641 agactgacta acacttccat aattagctct taaatatcgg tatgccgaat ccatcgcttc
     5701 tcttgctcca gcatctgaat tgatcccagt acgttcaaac tttccatttc ccgaagtcat
     5761 ttgcgtttcc aatttatagg tacctaattg cccagttttg ccataagata ccgtgtacaa
     5821 atgccctggc ttccctaacc cttctggaat caaagttcct ccaccttgtt caggaacaga
     5881 aacatactct tctcgctctt cttctaaatc aatgtatgaa aaattgacat cataaaattc
     5941 cattccacca attttcttta attgttcttt tacacgacga cgcatttcaa gagcaaatct
     6001 tagtatttct tcgacatcac ttttttcaaa tctaccatca ggatacaaca gtttcatcat
     6061 cccagataca atccgacgaa ctgcaatcgt atcccgctga tttaattgag aaccaagttt
     6121 gaaatattta tcaaaactat cgccaaaact aacttttcga agctcacgca tgacttcaga
     6181 aaaataatct gtaatgaagc catagtcatt tgtaaaaaaa tctggtcgat atttaggaat
     6241 ttcccaacct ggtaaataac aatgcatccg atccaaaaag gctgaatccg tccccataac
     6301 ctccggaaat ggctcaaata aattcgaagt tttcaatatt acatcaacgc tttgattaat
     6361 atttcctaca aaagccattg atgctgaagc attcttttct tctttgcctc tagcaaaaga
     6421 tcctgaagcc atgtaatctt tcatgatttg aacgccatca ttatccttaa atttaatccc
     6481 agcaacttcg tcaaaagcaa cacagtccca catacctaca agtcccattg tacgactagc
     6541 catattatag aaaaggtttg ctactgttgt ttgtccacct gaaacaagga ttgagtttgg
     6601 tgaaatttct ttatatacat gcgatttacc tgtaccacgt ggtcctagtt cacagagatt
     6661 aaaattattt tcaattaatg gaataagtct tgctaaatga agccatttta cccttttatt
     6721 aaattgtgta ggttccatac caatggaccg tagaagtaag tcaatccatt catttgtagt
     6781 aaactccttg cgagccatct tgtaagcctc aatgtctaaa ccaggcattt ggattggatc
     6841 aacctttttt gcaataaaag ggctattcca acgatcattc tcatcatacc aatattccat
     6901 cgaaacaata caccagatcc ccccacctaa taatcgatca aattttttcg gatactcttc
     6961 actaatttga acatttttta atcccaggtt agagaattct gcttcataac aatctttctt
     7021 ttcatttaaa tgtacagtta ctttatcaat aactgtataa ccacccaatt cacgaatttt
     7081 tgcttttact ttttctgctt catcaggacg cacatagttt cgagcaagaa tatttttaac
     7141 attttccaca cctttttcaa ccaatgtttc atccaatgac gaagcataca taccaagtaa
     7201 atattccaat acataaacag gtacattcgc accttcttta attttctttg ttaaatcttt
     7261 tctgacaatc tttccgggaa agaattcaga taactttaaa atttcctgtt gttccactag
     7321 aattcctcca tgtgttcagt cattataata gcaattaaag tatatcatac ttaaccagta
     7381 ggcttgataa tacaaaagaa aaagtatcca caaaaaattg tggatacctc gtttttatat
     7441 atcaaaatca aatgattctg caaaaggatt atctataatg aattttattc gctcggtcaa
     7501 atcatttttt tgatttttta tcactaaata ataagtatct tcaactgtat atcgacgatt
     7561 tacaaattca aaaacaaatt tagtaaaccg ctgatttgca tcttctgttt ttgaatccgc
     7621 aacatagatt tcttcatttg agatacgttc accatattca tcttcaaaat aaagccagaa
     7681 agatgcgggg tcataaacat cactaattgc atttttttga tagaattcta acgtggttga
     7741 taatcctgta attctgcggt tgctagtcat taattcaatt gctactggtt cctttgcatt
     7801 gctacctttg gctaccgtaa tttctagaac agggactagc atttcttgaa tagatgaccc
     7861 tccatgaacg tagctttgtc ccccgccagc tttcttaaaa atacttgaag taatcgggac
     7921 acaaatgttt ctagtgtcat catttcctaa agaatctccc aaagagtaat ttttcgtacc
     7981 aattatttcg tattcttctt cactaagaat aaaacgacgt tctacacgat ctttttcatt
     8041 tctagatgga ttttcaatct tatctacttc ttcaacgtgg cttcttttat aaataaatcc
     8101 atgatcagcc gtaactataa aacgatagat actgccatca ttatgcaagc gattaataag
     8161 tcgggaaatt tcgcttatcg ctaaatcaca ggcctcaaaa actcgatttt cattcgagtg
     8221 atcacctgtt ctatccactt gatcgtgata aatataaatg actgatttgc cattaaatac
     8281 ttctcgtacc tcagccttat tcatcttaac cacatcatca taatttaaag ctacagcatt
     8341 tgcatctcgt tgctggagaa tcgcatttcg attggataaa ccattcgttt ttttcccatc
     8401 aatcagtact tctgttcctt cattgtaagt tagttgattg tttggtaata aggcggcttt
     8461 accgaattca gtaactgaag gtaatacaga aagaaagcta gccatgtttg ttgaatactt
     8521 cttttctcta actaacttac gttctaaatt catccctaac tcatatcgaa aagcatctga
     8581 aataataact acagttttaa cagagcgttt cattacagct tgattataaa attttcgaat
     8641 ggatggacga ttcttaaatt caaaagtttg attccaaatt tgtcctgatt gatcaagaaa
     8701 cttcgaataa tttgcttcaa ctaatcgata caattggcca tatttttgct tttcttctat
     8761 atgatcacta ttccaaataa atttccgata atgcgtatca acccaataca tttccttctc
     8821 atattgagtt agcaaattat ccatgttcat cggcaagcac aatctgtttt tatccacaag
     8881 ataataagca tgtttcaata attgatactc ataaatataa tccgatgcga aatgcatttt
     8941 ttcgcgctgc tttaaactat caaaaattgt ttgtcctgca attttcgttg tagtgtcaga
     9001 agcctttaat cgttctgcat aaaacattaa tatttttgca tgaatctcag aaaatacgtc
     9061 tgcttcaatt agatcatcaa tttctatact gtctaataat ccatcaccat caatagcctg
     9121 atatactttt ttagatagct gttcaaagct ttccatgtaa cgtgtatcat tcataaactg
     9181 atccatgaaa gtaataatgt ttgctttcaa atgtagtatt tcaaatcctt tgaacttctt
     9241 cggcatctct tgatcaattt gataataaaa atagttcaaa taaagacaca tcgtcagctt
     9301 ttccaatgtt ggctggctat ccgtaaagcc aaatttcatt tgaataaatt cccaaaattt
     9361 agctgttaac tgatacttat caagttccct taaaggattc tccctacttt ctgtaaagct
     9421 ttttagaact acacgcagta aatcatttaa tcgaatactt ttcgatttca acaaaacagc
     9481 cattatcatt aattcaatcg tttctgattt attaattgtc atatgcgtac taaattcacc
     9541 aaaacttctc atgcgatcct ttgcattaaa gaaattacga tactgtttaa ctgtctgatg
     9601 aaatctaagt ggaatagcca attcattcat gataatcgct aagcggtctg cactaaattc
     9661 ctctgaataa ttcaatacac tcaacaaatg attatcttca tctttatcat cccaacgagg
     9721 aaatggcgca tagattaaat agttagtttc gggatcttca tattccaata aacgcttagt
     9781 tttaaattgt gcgtcctttt ctaatcgata gatttttgca ttttgaatga ccaatgagtc
     9841 aatttcttca agaaaatcct gatttgaatc aaaccaaaaa atgattttcc tacatccata
     9901 attaggaaaa cgttgattta atttttcttc aatttgttgc gtatttatat ctgtcatcaa
     9961 tctcacctaa tcgttctctt tctatacccc ttatattgac ataatttgga taatttcgca
    10021 cgcaatttaa gaataaaaaa gaaagttgtt agaaaacaac tttcttagat aactattttt
    10081 ttggttcttt gacccctttt gcaaaaattt ggaatttctc gccggtttta ggatcacgct
    10141 gcaacttatc ataattgaca attacaccat catctaagtc taattcaatt cgttttaatg
    10201 ctaagtgacc caacttctcg tcatactcac gaacttcttt taattgcttc tgaatcttta
    10261 caatttcttt tgtcattgca tttctttctt ttgggttatc acttgttgaa atttgttgct
    10321 gacgcaattc gattcgattg tcatacgctt tttgtagttc gtgtaggtat tcagtacgta
    10381 cttttcctaa ttgatctggt gtatagcgat gcaagtacat taacgcttta aagccatttt
    10441 gttttcctga gtccaattgc caataaattg gtcgattgga gtatgttttt aaatggtctt
    10501 tgtaaaaatc ttgtacgaag taacgacgaa taatttctcg acttgtccct ttgccaccta
    10561 aagtgtcagc gataaactgg agattttctt caagggtttc tttaccaaaa gtaatctcaa
    10621 cgaaatcaat gaaacgattg acaatgtcaa tttggctatc cttgaaatac tcatcatcgg
    10681 tgatgagtaa aacattatca gtatcaggtt taaatttttg atagcgggaa ggatcccatt
    10741 cgccgccagc gaagatgaga ccatcttcgt ctagggagta acgaccgaag atacaaccta
    10801 ttgaataaga aataaaatcc ttaatttccc tcgccttatc aggtaacctt gcagagatat
    10861 attcatcttg aacctctttt gttaattctt tttcaagaga ataatgatta atgaatatgt
    10921 cgttcagttc ctcttcattc gattttagag tattaaattg attaaccaac tgcctactta
    10981 gacagttaaa tgcctctgag agatcttttt taaacatata acttaacaaa atgtttcttc
    11041 taaactcgta tgacgtttca agagcattat agtcttttct agctatatcg atattttcat
    11101 ccactttttc tcttattttt tttaattttc tctcagatgt aactttaaga aaaggtagtt
//
LOCUS       DAZUMT010000097        11094 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000097 DAZUMT010000000
VERSION     DAZUMT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.098"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8FM_002293"
     CDS             33..656
                     /locus_tag="ACG8FM_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094938.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8FM_002294"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8FM_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5094939.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8FM_002295"
     CDS             2152..3033
                     /locus_tag="ACG8FM_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5094940.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8FM_002296"
     CDS             3036..3806
                     /locus_tag="ACG8FM_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094941.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8FM_002297"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8FM_002297"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS5094942.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8FM_002298"
     CDS             5117..6280
                     /locus_tag="ACG8FM_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5094943.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8FM_002299"
     CDS             6397..6807
                     /locus_tag="ACG8FM_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5094944.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8FM_002300"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8FM_002300"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5094945.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8FM_002301"
     CDS             9380..9664
                     /locus_tag="ACG8FM_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094946.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8FM_002302"
     CDS             9824..10060
                     /locus_tag="ACG8FM_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5094947.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8FM_002303"
     CDS             10057..10455
                     /locus_tag="ACG8FM_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5094948.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3876 a         1834 c         2304 g         3080 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt ttta
//
LOCUS       DAZUMT010000098        10623 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000098 DAZUMT010000000
VERSION     DAZUMT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.099"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG8FM_002304"
     tRNA            8..81
                     /locus_tag="ACG8FM_002304"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACG8FM_002305"
     tRNA            101..173
                     /locus_tag="ACG8FM_002305"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACG8FM_002306"
     tRNA            201..274
                     /locus_tag="ACG8FM_002306"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACG8FM_002307"
     tRNA            288..361
                     /locus_tag="ACG8FM_002307"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACG8FM_002308"
     tRNA            385..474
                     /locus_tag="ACG8FM_002308"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACG8FM_002309"
     tRNA            487..560
                     /locus_tag="ACG8FM_002309"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACG8FM_002310"
     tRNA            579..654
                     /locus_tag="ACG8FM_002310"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACG8FM_002311"
     tRNA            656..726
                     /locus_tag="ACG8FM_002311"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACG8FM_002312"
     tRNA            740..813
                     /locus_tag="ACG8FM_002312"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACG8FM_002313"
     tRNA            838..925
                     /locus_tag="ACG8FM_002313"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACG8FM_002314"
     tRNA            947..1018
                     /locus_tag="ACG8FM_002314"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACG8FM_002315"
     tRNA            1041..1130
                     /locus_tag="ACG8FM_002315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACG8FM_002316"
     tRNA            1143..1216
                     /locus_tag="ACG8FM_002316"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACG8FM_002317"
     tRNA            1235..1307
                     /locus_tag="ACG8FM_002317"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACG8FM_002318"
     tRNA            1311..1383
                     /locus_tag="ACG8FM_002318"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACG8FM_002319"
     tRNA            1388..1458
                     /locus_tag="ACG8FM_002319"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACG8FM_002320"
     tRNA            1471..1544
                     /locus_tag="ACG8FM_002320"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACG8FM_002321"
     tRNA            1556..1627
                     /locus_tag="ACG8FM_002321"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACG8FM_002322"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACG8FM_002322"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS5094949.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACG8FM_002323"
     CDS             3216..4688
                     /locus_tag="ACG8FM_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5094950.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACG8FM_002324"
     CDS             4771..5559
                     /locus_tag="ACG8FM_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5094951.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACG8FM_002325"
     CDS             complement(5626..6294)
                     /locus_tag="ACG8FM_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS5094952.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACG8FM_002326"
     CDS             6409..8121
                     /locus_tag="ACG8FM_002326"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094953.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="ACG8FM_002327"
     CDS             8124..9896
                     /locus_tag="ACG8FM_002327"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5094954.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(9978..>10623)
                     /locus_tag="ACG8FM_002328"
     CDS             complement(9978..>10623)
                     /locus_tag="ACG8FM_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5094955.1"
                     /translation="SLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLI
                     TKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNA
                     KEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHML
                     RHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3097 a         1865 c         2370 g         3291 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtatct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag ttgattttgc
    10021 atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc atgtcctaca
    10081 cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat gtgggaatgc
    10141 cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac tgctcttaaa
    10201 gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa aatgtattta
    10261 tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat ttctttagca
    10321 ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt tttaattttt
    10381 aactcgtaat cataagtacc agaaaccgta attttattac catcataatc agattcagtc
    10441 aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt aattaaagaa
    10501 taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac ttcttctttt
    10561 gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt tcttgctaaa
    10621 gaa
//
LOCUS       DAZUMT010000099        10318 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000099 DAZUMT010000000
VERSION     DAZUMT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.100"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..904)
                     /locus_tag="ACG8FM_002329"
     CDS             complement(71..904)
                     /locus_tag="ACG8FM_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS5094956.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1208..3373)
                     /locus_tag="ACG8FM_002330"
     CDS             complement(1208..3373)
                     /locus_tag="ACG8FM_002330"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS5094957.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3633..5927
                     /locus_tag="ACG8FM_002331"
     CDS             3633..5927
                     /locus_tag="ACG8FM_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS5094958.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5920..6585
                     /gene="pgmB"
                     /locus_tag="ACG8FM_002332"
     CDS             5920..6585
                     /gene="pgmB"
                     /locus_tag="ACG8FM_002332"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5094959.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6578..7585
                     /locus_tag="ACG8FM_002333"
     CDS             6578..7585
                     /locus_tag="ACG8FM_002333"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5094960.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7607..8626
                     /locus_tag="ACG8FM_002334"
     CDS             7607..8626
                     /locus_tag="ACG8FM_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5094961.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACG8FM_002335"
     CDS             complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACG8FM_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5094962.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         3198 a         2242 c         1909 g         2969 t
ORIGIN      
        1 ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat
       61 gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga
      121 ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga
      181 cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt
      241 ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc
      301 aactttctct tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga
      361 aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa
      421 agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat
      481 catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa
      541 attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt
      601 cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt
      661 gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat
      721 tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt
      781 ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt
      841 cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt
      901 tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca
      961 caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca
     1021 ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct
     1081 ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat
     1141 aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
     1201 cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct
     1261 tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt
     1321 cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt
     1381 ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca
     1441 tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga
     1501 aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag
     1561 caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa
     1621 tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac
     1681 ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca
     1741 gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc
     1801 cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta
     1861 ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga
     1921 tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg
     1981 ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg
     2041 gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa
     2101 aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta
     2161 acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat
     2221 ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag
     2281 caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg
     2341 ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg
     2401 caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag
     2461 ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat
     2521 tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg
     2581 cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg
     2641 tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg
     2701 gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc
     2761 cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga
     2821 taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc
     2881 tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg atccctacga
     2941 atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga
     3001 tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga
     3061 caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg
     3121 ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa
     3181 gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg
     3241 tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg
     3301 gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac tcaaaactaa
     3361 atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc
     3421 gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa
     3481 aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct
     3541 tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg
     3601 aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta
     3661 ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat
     3721 ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact
     3781 caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg
     3841 tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact
     3901 ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag
     3961 aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca
     4021 ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag
     4081 aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg
     4141 tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga
     4201 atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa
     4261 acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa
     4321 aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag
     4381 aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag
     4441 atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca
     4501 attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg
     4561 atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac
     4621 tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag
     4681 gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc
     4741 ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc
     4801 cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca
     4861 ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa
     4921 atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt
     4981 tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc
     5041 atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg
     5101 agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca
     5161 cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac
     5221 tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa
     5281 tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca
     5341 caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca
     5401 tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag
     5461 aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc
     5521 caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt
     5581 accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc
     5641 acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa
     5701 ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt
     5761 tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt
     5821 tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag
     5881 cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt
     5941 gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt
     6001 ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc
     6061 cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag
     6121 gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg
     6181 atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa
     6241 aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg
     6301 ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct
     6361 gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc
     6421 gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt
     6481 ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt
     6541 cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt
     6601 cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt
     6661 cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa
     6721 atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt
     6781 ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca
     6841 tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt
     6901 ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt
     6961 aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac
     7021 aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc
     7081 gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca
     7141 gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa
     7201 ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat
     7261 ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc
     7321 caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc
     7381 taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg
     7441 aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt
     7501 tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac
     7561 gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt
     7621 aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga
     7681 tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg
     7741 ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt
     7801 aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat
     7861 ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa
     7921 aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg
     7981 ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca
     8041 catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga
     8101 taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa
     8161 gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg
     8221 ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca
     8281 tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt
     8341 ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt
     8401 cattccagag acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca
     8461 cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa
     8521 gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg
     8581 cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact
     8641 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
     8701 acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     8761 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt
     8821 cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt
     8881 ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa
     8941 ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc
     9001 aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga
     9061 gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca
     9121 acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct
     9181 ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt
     9241 cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt
     9301 aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc
     9361 ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc
     9421 atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg
     9481 agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc
     9541 ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg
     9601 ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc
     9661 gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata
     9721 tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc
     9781 gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg
     9841 aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat
     9901 gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa
     9961 cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc
    10021 catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg
    10081 attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa
    10141 aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg
    10201 atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa
    10261 atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaa
//
LOCUS       DAZUMT010000100        10101 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000100 DAZUMT010000000
VERSION     DAZUMT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.101"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            326..2161
                     /gene="tdc"
                     /locus_tag="ACG8FM_002336"
     CDS             326..2161
                     /gene="tdc"
                     /locus_tag="ACG8FM_002336"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5094963.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2466..3944
                     /locus_tag="ACG8FM_002337"
     CDS             2466..3944
                     /locus_tag="ACG8FM_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5094964.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3910..5241
                     /locus_tag="ACG8FM_002338"
     CDS             3910..5241
                     /locus_tag="ACG8FM_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5094965.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5355..7907
                     /locus_tag="ACG8FM_002339"
     CDS             5355..7907
                     /locus_tag="ACG8FM_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS5094966.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7914..9353)
                     /locus_tag="ACG8FM_002340"
     CDS             complement(7914..9353)
                     /locus_tag="ACG8FM_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5094967.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9328..9510
                     /locus_tag="ACG8FM_002341"
     CDS             9328..9510
                     /locus_tag="ACG8FM_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094968.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9699..10061
                     /locus_tag="ACG8FM_002342"
     CDS             9699..10061
                     /locus_tag="ACG8FM_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS5094969.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3222 a         1652 c         2137 g         3090 t
ORIGIN      
        1 taattctatt aaaaaataaa ttgcgaattt ttaacaatat aaaaaaataa aaatatgttt
       61 agtgaatgaa ggacagtcat tttatttgtt tgttcgcttt ttttaggtta tctattttag
      121 aagactgatt tattgaaaaa ttgttcgctt ttttgcgttg aattatcaga aaaatccgac
      181 tttatttatt tttgtttgta aaaatctacg tggatttgat gttataaaaa aaaaattctc
      241 atttttggcg ctatagtgaa gaggtgttac agataaacac atgtatctgc aagacattga
      301 ccaacaaaat atggagggaa gaaaaatgaa agatatggat atcaaggccg tctttatagg
      361 cgacaaagct gaaaatggac cagtttataa aatgttgttg aacaagatgg tagatgaaca
      421 tcttggatgg agagaaaatt atattccttc agatatgcct gcaattagcg aaggagataa
      481 gctcactcca gattatctag ccacccgcga ccatatgata gaggtcttgg atgaagtcag
      541 tcagcgatta cgcgcaggat cgatcccatg gcattcagca ggccgttact ggggacaaat
      601 gaatgctgaa acattgatgc ctgctttatt agcttacaac tatgcgatgc tgtggaaccc
      661 aaataatgtt gcattggaat cttctatggc aacttcgcaa atggaagcag aagtaggaca
      721 ggattttgct tcattattca atatgacaga cggatgggga cacattgcag cagatggttc
      781 tattgctaat cttgaaggat tatggtacgc ccgttgcatc aaatcgattc ctttagctgt
      841 caaggaagtg ctgcctgaaa aagtgaaaaa aatgtctgag tgggaacttt tgaatttatc
      901 tgttgaagaa attttggaaa tgaccgaaag ctttacggac gaagaaatgg atgaggtcaa
      961 agcagcttct tcccgtagcg gaaaaaatat ccaaagatta gggaaatggc tggtccctca
     1021 aacaaaacac tattcttgga tgaaggcgtt agatatttgc ggagtcggct tagatcagat
     1081 ggttgctatc ccagttcaag aggattatcg aatggatatt aatgctttgg aaaaaacaat
     1141 tcgtgaatta gctggacaaa aaatcccaat tctaggtgtg gttgctgtcg taggaacgac
     1201 agaagaaggt caagtagaca gtgtagataa aatcgtccaa ttaagagaaa ggttaaaaga
     1261 tgaagggatc tatttctatt tacatgttga tgccgcatat ggcggctatg cgcgttcact
     1321 gtttctaaac gaagcaggag aatttgtgcc gtatgcttcc ttagctgaat tctttgaaga
     1381 acatcatgtt ttccaccact gcgtaacgat tgacaaagag gtatatgaag ggttcagagc
     1441 tatttcagaa gcggattctg tcacgataga tcctcataag atgggctatg tcccttatgc
     1501 tgctggagga atcgtaatca aacataaaaa tatgagaaat atcatttctt atttcgcgcc
     1561 atatgtgttt gaaaaatcag taaaggctcc agatatgtta ggagcttata ttttggaagg
     1621 atcaaaagct ggagcaaccg ccgccgcagt ctggacagca catcgtgtat tgccattgaa
     1681 tgtgaccgga tatggtcaat tgattggggc ttcaatcgag gcagctcaaa gattcagaga
     1741 atttctcgat catctgactt ttactgttaa aggaaaaaca atcgaagtct atccattgaa
     1801 tcatcctgat tttaatatgg tcaattgggt attcaaagaa caaggctgta cagatttgaa
     1861 cgctatcaat gaattaaatg aaaaaatgtt cgatcggtct tcttatatgg atggcgatgt
     1921 ttatggtgaa cggtttatta cttctcatac tacatttaca caagaagatt acggtgattc
     1981 tccgattcgt tttgttgaaa gaatgggtct aacgaaagaa gaatggaaaa aagaacagaa
     2041 aatcactctg ctacgtgcgg ctatcatgac tccttatttg aatgatgatc gaattttcaa
     2101 tttttataca aaaaaaatcg ccaaagcaat ggaaaagaaa ctaaacgaaa tcatccaata
     2161 gaactgattt ttgtgtgaaa gaataacaag agacatatgg agaacagaca attatttgct
     2221 tcatcaaaac aattgtattt ccccctgttt ttcatatatt cctcgccaag atattctttt
     2281 ttataaaacg ttctataagt tggatcgttc gtttcatgta tcaaacccag ttcactaaga
     2341 tcgttcaatg gatgtttcgg tgatactggg ttttgtactg aatcgatgaa accttgtcat
     2401 ttctgttctt gaacaaaaat aaaagaaaac aaaaacatat gtcgctattg aaagaaggaa
     2461 taaagatgaa agatactggt tcatcaaagc aaatcacgac catgaccttt atcggaatga
     2521 cctgcgccct tgtagcaagt gtacgaaata ttccagatgt agcagcaacg agctggacga
     2581 tgatttttta catgctggtt gcagtattat tgtacgcttt tccaatctca ttgatctctg
     2641 gtgaatttgc tggaatgttt ccccaaaaag gtggtccaga actttgggta tcgaatgctc
     2701 taggaaaaaa atgggggttc gttgtctcat ggctgttatg ggtccaaatg tttcctggga
     2761 tggtgatggt cgcttcagca ctcgctccgt tgtttggtaa tattatcgat aatgtgccgc
     2821 ttggtttgaa cagcaaattt acattagttg tcattttagt cgtttattgg atcatcactt
     2881 ttttgaattt gaaatttgat atggcaaaaa tcggcggaaa agtcggtgtt tggttaggat
     2941 tgtacgtgcc tttgacgatg atgctcttat tagggtttgc tgcgtggatc aaagttggga
     3001 tcgtaccaac cggaacatta ggcagttttt catgggacaa attaatacca gatactacga
     3061 ctgcatcctc atttgtttat tttgcgccga tcatgtttat ttttactggt atcgaaatgt
     3121 cctctgtgta tatcactcga ttggaaaatc cagttaaaac gtatattcgc ggagtatttg
     3181 ccgcgttaat ttttatattt ttcgtcaata ttttgaatgc tttagttgta gcaaatgtcg
     3241 taccaaaagg acaaatggaa ttgaataata tcgcgcaatc tatttcgatt tattgccaaa
     3301 ttcttggttt gccacatctt attgtgaatc tgtttagttt acttgtcttc attggtgtag
     3361 cagtccaatt atctgcttgg gcctcaggac cagcaaagac agtgacagaa agtgctagaa
     3421 aaggtgcgta tccaccaaaa ttcaattttt ggaagacgaa tcaatttgac gtatccaaat
     3481 ccgttatttt gactcagtct gtcattatct caatctttgc acttttttat ttgctgattc
     3541 ctggcgtcaa tcaagcattt ttgatgctcg taaattcaac tactgttatc tactgtatcg
     3601 tctatgtcat catggggatc gcagttttga gattgcgtta tacacatgca aagttgaatc
     3661 gaccatttag aattggaaaa aaagagaaaa agagcaatct tggagtttgg actgtcgtta
     3721 ttgttttatt tgcggcaatc ggattttctg tcggactgac gatgaaagct ggaacatgga
     3781 tcaatctaat tgcagtcaca gccatcagtg tcattttgtt tgtagtacca ctctgtattg
     3841 aaaaaataaa gaaaccttct tgggaacagg aagtcgccat gctgcttgaa aaagaaaaag
     3901 gtgaacttga tggaaaaacc ggcaatgaaa cggactctaa ctaatcgaaa tatccagtta
     3961 atcgcattag gtggcgcaat tggaacagga ttatttttag gttcagcaaa ggcaattgca
     4021 tcagcagggc cagctgttat tctcgtttat ttgatatgcg gtttggctat ttatggtttg
     4081 atgcgggcca tgggtgattt atttttagca aatagtacat ttaactctat cagtgatttc
     4141 gttgactatt atttaggcaa aaagtgggca ttttttgtca attggactta ttggctttgc
     4201 tggcttggag ctggacttgc ggaattaaca gcaataggat tgtatatcca tttctggttt
     4261 gtacatgtac cagctttgat aagcggaggt agcgcactaa tgatcttaat ggcagtcaac
     4321 cttgttgcag tcaagtggtt tggtgaattg gaatcttttt tctcaatcat caagatcttg
     4381 gggattttcc tttttatctt attgggtata ggattgagtg tctcagattt tcatacccag
     4441 tctatcggaa tcaaagaaaa ttttggacag atcgatgaat tactaccgtt tggctttgct
     4501 ggaatggtag tctcatttcc tatggtttta ttcagttttg ctggaattga aatgatcggc
     4561 ttaaccgttg gcgagacaag tgatccgcaa aagaatttga aaaaagcaat caactgtctg
     4621 ccttggcgta ttctcttttt ctatatagga acgattttgt cgattttatt tgttgttcca
     4681 tggcattgtt tagatttaaa agaaagtcct ttagtgacta tgctgcaagt gatccattgc
     4741 caaggcggag cagcaattgt caatctaatg attttggttg cttctttgtc ctcagcaaat
     4801 agtgcagtct acagtactag caggatttta tatcaaacag caaaagaaaa aagtttgccc
     4861 cattggtttt gtgcgctttc taagaaaggc gtacctttac acggtatttt aattacagga
     4921 atgttatttt ttgccggtct ttgtctacat ttttttgtag ctgatagtcg attgtttttt
     4981 cagcttttgg caggcatgac aacgagttgt tttctatttg tgtggtcttc gatcttgtgt
     5041 gcacatgttc aatttattaa agaaaagaaa cggaacacta cacaaagata taaagatctt
     5101 gctttgttgg tgttttttac ttcaattgct ttcagcttgt tattcgaaag cacgacacgc
     5161 tttattcctt ttttattggt tatctggttt gtattcttaa attttgtata taaaagaata
     5221 agcaatagaa atatcagtta ggcccagata cattttgcaa aacgaattat ttcagacccc
     5281 cctgaaataa tcgatacgcc tgaaaaaact tccctgcgta taataacagt atcaattaaa
     5341 caggagggga taggatgaac tggtacagat tgacggtaga agatgtattg aaaaactatc
     5401 aaacatcaac agaaggcttg tcgcaagaag aacgacagaa acgcctgact gatcaaggat
     5461 ataatgaaat aaaagcaagg cagcagacaa aacgatggaa aaaaatagca aagcatttta
     5521 ctgatctgct gatgatcgtg ttgatcattg cctctttttt gaaatttgct tctagtgaat
     5581 atattgaagg aagtattatt ttattcgtag tgatcgtcaa tggactcgtt agttactggc
     5641 aagaaagaaa agcggaagaa tcattgaatg gtttgaagca gctgatggga caagaagcga
     5701 tagtactatc aaacgggatg caagaaaaaa tccctgcgaa agaactggta aaaggagatg
     5761 tcattgtctt acgtgccgga gatgtcgttc cagcagatat ccgattaatc gaagcacatg
     5821 atttattagt ggaagaatcg attttgacgg gagaatcaga agcatctgaa aaaagtcatg
     5881 ccgttttgac agaagaagaa tcgatcggcg accagaaaaa tatgggtttt tcaggaacgc
     5941 ttgtacaatc tggttctgca gtcggtgtcg ttgtagaaac aggaaacacg acagaaatcg
     6001 gtaaaatcaa tcaagcattg caatcggtcg aacaacaaac gacgcctctg attaaaaaaa
     6061 tcaaccgatt aaacaaacaa atttttcaag gcattctttg tttgagccta ttcttagtta
     6121 tttttacgac atttcgatat ggtatggatt ggcatattct tctttcggct acgattgctt
     6181 tggtggtgtc aatggtaccc gaaggattgc cagctgtttt gaccatgatt ctatccgttg
     6241 gagtacatga aatggcaaaa gaaaaggcga tcatcaaagg acttccttcg gtagaaacat
     6301 taggttctat gacagtgatc tgttcagata agacaggaac attgacaaaa aatgaaatga
     6361 cagttgtgga tgtagctgca aaagaagaag cctgcgtgtt gtccattatg aaaaactgtc
     6421 aggaattaaa aacaaaagaa gaacagaaaa cagaaaattt aagtggcaat ccgacagaag
     6481 ttgcgctgat tcgacacaca gaaacagcca atctaccttt gaaaaaaata gaagcaaaga
     6541 ttccgttcag ttctgaatac aaatatatgg caacgatgca tgcggaggaa aaaggagcgg
     6601 tgatttacgt caaaggtgca ccagaagtat tatttgctaa atcaactctt tcttctgcag
     6661 aacaagaagc atggagtcag acagctgcag aatttgcgag aaaaggtcag cgtgttttag
     6721 gatttgctta taaaaaagta gattcgaaac aggaactcac gcatgagact ttaacccaat
     6781 taacttttgc agggatcgcc ggattgatcg atccgccaaa agaaagtgca gtcaaagctg
     6841 taaaggaatg ccaacaagca gggatctcag tgaaaatgat tacaggggat cacaaagaca
     6901 cagcaaaagc gattgcggaa caaattggct taaaacatac tgccaaagtt ttagaaggga
     6961 tcgatttgga tcttatgtct gatgaagagt tgattcagca agtgccaata gtcgatgtct
     7021 ttgctcgaac gacaccagaa cataaactgc gaattgttaa agctttgcag aaaaatggag
     7081 aaatcgtcgg aatgaccgga gacggcgtaa atgatgcacc tgctttgaaa agatccgatg
     7141 taggaattgc tatgggaatc aaaggtagtg aagtaagcaa acaggcagct gatatggttt
     7201 taggagatga taatttccat acgattgcaa aagccgtaaa agagggccgg cggattttgg
     7261 ataatttgca aaaaaccatc aatttcttct tgccaactgc attagctcaa ggattgatct
     7321 tgatttgggc acttatgctg aatcgcccgc tacctttgtc cccagtacag attttatggg
     7381 tcaatatggt aacgacaatc actttatctt atgcactagg gttcgagtcg ccagatccag
     7441 aaatcatgaa gcgcccacca agagatccaa agcaaggaat cctatctggt tatcatttgt
     7501 ttcggatcat ctatgtctcc ctactgatca tggttcctgc ctatttattg gctatgcagt
     7561 ttgaagggca aagcttacag caaaccattc ttctgcaaaa tatcgtctta gctcaagctg
     7621 tttacatgat caattgcaga gagttgtcca agccgtcatt gaaccaaggt cttttgcaaa
     7681 acaaagcgct ttttctttca ttaggtatcc ttgctttatt gcaagggatg gttatttttc
     7741 taccagtcgg ccagcagctg atcggtacag taagtttaac gatgacacaa cagttattga
     7801 ttggagcaaa tgtgattctt ctattcttag ttgtagaaat agaaaaaggg atcatgaaca
     7861 aactgtttcg caaaaaagaa aaaacagcta gagaatacag tcgctagccg ttactataaa
     7921 aaagttttga aagtgagttc agtcaaacga ttgagctcat ttttcgttgg aatagaagaa
     7981 atgtatacaa tagggatatc tagagaatct agagggaaat tggaaatcaa gatatctgtt
     8041 ttttcaaaag aaatagtgga aagatcggtt agttcgatag gtaagaagca gacacgtttt
     8101 cctaagtaat cttttaattc ctgctgcaag aacgccttcc atgaaggttc tccttggaaa
     8161 ataaaataga cattgaacaa attcggtttg atagaaaaaa tcgtttgttg gatcagtaga
     8221 gacaagagtt ggagaaagta atttggttca ttgatttcta tttctttttt tagatcgttc
     8281 agtaaattct ctttctgttg atacaagtgt gcatattgct gatcatatgt ttctaaaaaa
     8341 cgatggtatt gacggtccaa tgcttcaatc aatatcggcg attcatgata ttttaatatg
     8401 aggagagcca agttatcagt cagagatgtt ttttttagtt gatcaagaga cagctctgaa
     8461 gctagttcct ctacaaacgt ttcgaggacc tctaaaaatg gagaatgatg caggcagcga
     8521 gcggtttctt gtccatagtt attgttgtaa ttccaacttt ccagaaagca gaaagaagca
     8581 aaagaaagct cagtgtcttt aagataaata ccttccgtta aatacaattt tttcaatgca
     8641 gagtcataca gggaggagag tgtgctgttg gtatggatag aagaatctaa tcggcaattc
     8701 gcttttatac ggatcgtatt gataaagaac caacatgttc cgaaaatctc ttctgtttca
     8761 accgctagtt cagtttgact gaggtttctt aaaaattgaa aataatggga ttcatggatc
     8821 gaataatcct cgaaaaaata gttgttatga gtgaaaggaa tcagcaatcg atgataaaaa
     8881 gtacgaatcg cacgctcttt tccaagaata caagctgtat atttcgtcaa atcgatctgc
     8941 aagtcgtaac ttgctaaacg ctgattgatt ttagtcacat gtcttttcaa tgtcccataa
     9001 gaaagcccat ggtcggctag aaaatcaggg acagcaactt tttttgtaaa aagcagattt
     9061 ttcagcagtt cgacactgac tgattgtttg aaaaaaatct cccaaaggtc atttgttaat
     9121 aaattttgct ggttttgcaa actgattcct gcttgatcgc tgatgatgtc ccagccttcg
     9181 ggcagctgag agcgaataga ttgaaggtca gaaaaaatcg ttcgttcggt tgtccctatg
     9241 atttcagcta attcatttac ggttatcttt gtatgattca gcaattgttc tactaaagaa
     9301 acatgacgtc gaatatcttt ttcggtaatg atttttttca ttagtgaata catcaatcag
     9361 actcccttct attctaagaa tacggagaaa ataaacggtg gtcaagtttc aactgatttt
     9421 tcaatcattg tttatttttc tggagcattt actgatgata agaatttcat catgaacttg
     9481 aaaaatgata ccactaaaat caaaaaatag gtgggcaaac aataataaac attttaatag
     9541 tagatggtag caagaccaac acaggcggat tgaacattta agagttgata aaaaagaaaa
     9601 atatcgaagt atttgattag caactacttg tattcatttt tctattctta cgtggtatct
     9661 ttgaataaag cgaatcaata agaaagagat ggaagcagat gctaacgaaa aaagaactaa
     9721 tcgatcacat caatgaaaca tacaatttgt ctccagaata tatttttaaa aaatttcctg
     9781 attattgcgt attcagacat caaaaaagca aaaaatggtt tggggtcctg atgtctgttc
     9841 cagaagaaaa aatctatgat gatgcaagtg accataggat catcaatatt cttgatttga
     9901 aggtcgatcc tgaacttggc ggaattttgc gaggaaaaga ggggtatttt ccagcctatc
     9961 acatgaataa agaacattgg atctctattg atctacgtaa aatagaaaag ctagatgaac
    10021 tagcagattt gattgatgga agttttaaat caacgcaatg aatgtctagg acaatagaga
    10081 agaggttgtg acagaaatgt t
//
LOCUS       DAZUMT010000101        10021 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000101 DAZUMT010000000
VERSION     DAZUMT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.103"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(487..711)
                     /locus_tag="ACG8FM_002343"
     CDS             complement(487..711)
                     /locus_tag="ACG8FM_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094970.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            902..3679
                     /locus_tag="ACG8FM_002344"
     CDS             902..3679
                     /locus_tag="ACG8FM_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5094971.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3760..4179
                     /locus_tag="ACG8FM_002345"
     CDS             3760..4179
                     /locus_tag="ACG8FM_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5094972.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4191..4661
                     /locus_tag="ACG8FM_002346"
     CDS             4191..4661
                     /locus_tag="ACG8FM_002346"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5094973.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4678..5457
                     /locus_tag="ACG8FM_002347"
     CDS             4678..5457
                     /locus_tag="ACG8FM_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5094974.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5444..6265
                     /locus_tag="ACG8FM_002348"
     CDS             5444..6265
                     /locus_tag="ACG8FM_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5094975.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6282..7331
                     /locus_tag="ACG8FM_002349"
     CDS             6282..7331
                     /locus_tag="ACG8FM_002349"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5094976.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7344..8348
                     /locus_tag="ACG8FM_002350"
     CDS             7344..8348
                     /locus_tag="ACG8FM_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5094977.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8981..10000
                     /locus_tag="ACG8FM_002351"
     CDS             8981..10000
                     /locus_tag="ACG8FM_002351"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5094978.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1651 c         2093 g         2915 t
ORIGIN      
        1 ttaatttgtt cttgcaatct tttttgctac gtaatttttg taaaaaagtg ggttgagaga
       61 aatttaattg attttcaaag aaggagtatg aaaatgagaa aaatatttat tcgtttattg
      121 tgggtactat cttagaggaa taaataatag tggagaaaac tatggtttgg cgcagattct
      181 atcaggtttg atggaacgaa gacaacctcc cgacatcaag tcaaaagcca cttgagattt
      241 tgatctgaaa ataaaaaaag aaaacaaggt aggaacatca atgtttctac cttgttttcg
      301 taatcaatgg gaaaaaacta agagtggtgt acgtaatcaa taaaaaggat ggtgacaaca
      361 gtacctcttt actctctgaa agcatcataa ctcgtttttc ctcattatgc caataaattg
      421 ttgttatacc acatataagg atttgtaata gctaaaaaca gacaggaaag ttgaaatctg
      481 tgaatcttat tttgtatgga aggatttata gaacgcaaaa atcaagataa agatgaataa
      541 aaatcctgtt tcggctatat attgcgtatc tacgccaata aaacgagttt gttctgtcat
      601 agccatataa agcaaaggca aaaatacaac tacagcgaga taagatccga tgatttctcg
      661 aaaagttttc caggaagtat gtttcctgtt tttatattta tccgatttca ttgaatagtc
      721 atagcctcct tattctttca tatgttgaaa ttccagtata aaaactggaa ggaactgtac
      781 gttttttaga ataaaaatta taagggataa gtaggctgct gtctataata agatatattt
      841 ttcaattctt tattaaagat tttattgaca aaatgaatcc gttttctatg atggaggagg
      901 ggtgaataag ttgttgaaga aggaaatttt atgttactta gagaaccaga cagccttttt
      961 cgatttagaa catatgaacg aggtctttac agcacaaagg ttagctgatc attttggggt
     1021 aaaaagaaac acagtcagtc attatttgaa ccagttaaat gatgagcaac tactagtcaa
     1081 aatcaattct cgaccagtga tttatttcca caaagaagct tttgaaaaac aattttttaa
     1141 gttacgcacg aatttttatt tttctatcga tgatctcaaa gaggaacagc cttttttcgc
     1201 gcaaccaaaa gacttattct cttttatgat cggatacgac gccagtttga aagaaagtat
     1261 cgaacaaatc aaaacagctc tttactatcc agatggcgga ttaccacttt tgattacagg
     1321 cgaaagtggc acaggtaaaa gttatttagt taatttggtc tatcagtatt gtctgcttca
     1381 tgatctgcta gaagattctg caccattcat cacttttaat tgtgcacaat atgcagataa
     1441 tcctgaactt ttgactagca atttgtttgg acatgttaag ggagcgtaca ctggtgcaga
     1501 agaaaataaa aaaggggctt ttgaagcagc agatggtggg atcttgtttt tagacgaggt
     1561 acatcgctta acaccagaag gtcaggaaaa actgttcacc tatcttgatc aaggtgtgat
     1621 ttatcgtatg ggagaaccta atttgtcacg ccggatcaaa acacgtctct tttttgctac
     1681 gactgaagag attactagtc atttcttaac aactttcatt cgaagaatac ctatccgaat
     1741 cgaactacct gcacttaatc agcgtagtcg taatgaacgg ctagaactga tttactcttt
     1801 ttttctcgaa gaacaaagaa aaatggggag ccctttgtca gtcagtggac cagctttgtc
     1861 acttttaaca agtcagaaat tatccggaaa cataggagag ctgaaaaata tcgtcaaagt
     1921 ttcagcagca aaagcatatg ctgaacagcg tgaacagcct gaaatccatg tcacgatcca
     1981 tcatttgcaa aaagaactac ttgcgcagcc tattactaaa aatacagcac agcaaggaat
     2041 ctatattacg caagaccaga ccttagagca attaatggag aagcaacagc ccgaacagca
     2101 gcgaatcgtt aaaagttttg agcaaatcct aattgatttc aagaaaaata actgtctgct
     2161 atattcttgc gaaggaaaat taaagcaaga agtgattcaa ttatttgatt ttttgctgtt
     2221 tgagaccagt agacaagaaa aacacgagct gctggtttat tggacacaat ctatccgaga
     2281 gacctttcga caaatggaaa ctgcctatca aatcaaattc gatggaaaca gtgtctatgc
     2341 cttgagttac tatctctatc agcgaagcac tatcaaatgg ttaccggaag aacatgatat
     2401 tatccaacta atcaaagaat tagaacaaca agtttctcag tcttatccat ctagttatca
     2461 ctatgtccaa cgaattctgg aattaagtaa gatcaaatta gatattgaag acacaagtat
     2521 ggatcggatt ttactgacga tctatctaaa aaaagcgaaa tggtcaaaag aaaaacgact
     2581 gccaaaagcg atgattgctg ctcacggtta tgcaactgct agcagtatgg caaatgtagt
     2641 aaatcgttta ttacaagagg atctatttga atcatttgat atgccactag atgtcacacc
     2701 acagcagatt gcttcagaaa tccttgatta ttgcgaaaac agcgatgttt caaatggatt
     2761 gatcatatta gtagacatgg gatctctgaa agaaatccat cagtttttta aaaaacagtt
     2821 atccgtaccg cttttgattt tgaataatgt gacgacacct ttagcaatca cagtagggga
     2881 atgcctgcaa aaaaatgtat cattagaaga gatagcagaa gagactgttc gacagatcca
     2941 gccagaatgg cgactgctct atccagagga aaacaaaccc aaagcactca ttactacttg
     3001 cttcactgga atcggtacgg cggtccactt aagtgaactg ctagaaaaaa gcttacctac
     3061 ggcttgtcag ttgaaaatca tcccatatga atatcagcaa ttaaaagata agaaaaacag
     3121 tgacccatta ttttccatat acgaagtcgt aggaatgatt ggcaccacag atcctgatat
     3181 caccaatatc gactatcttt cattagaaga cctcatctcg ggggaaaaga tgtctgtact
     3241 agctgaatgg ttggacacta ctatgaatgc ttcagagaag gaaatattca atcagcgcat
     3301 cattcgtaat ttttcattgg aaaaagtact ggattccgtg actattctag atacagagaa
     3361 ggtgatggga gaaatcgatc attttatgag agaattagag gtgcagttga atcgaagcat
     3421 cagcaatccc aaaaaattag cgttgtatgt ccatgtgagc tgtctcattg aacgattgat
     3481 ccgacaaatg ccaatcgaaa cctatcaagg attagagaaa tggaagcagt gtcaaaaaga
     3541 agggttatct gctattaaat ctgcgtttag tgtcattgaa gagcattata gtgtcatgat
     3601 ccccaattct gagattgcct atatctatga tattttgagt gaagatacag agtttctctc
     3661 gatagaagaa gaattttgaa cataagcaag ttgttgccac taattttggc atgcaacttg
     3721 cttttttagt atgtgtaagt gaaaagagga gatgaaaaag tggatagaga aattatctta
     3781 gcttcccatg gaaaatttgc ttcagggata ttagattcat tagagctgat ctatgggaaa
     3841 aatcattcta ttactgtatt cgattgctat actgatgaga attttgattt agcggaaaca
     3901 gtaaggcagc tttttatgag gtacaaagat aaagaaatca ttgttctgac ggatcttttt
     3961 ggcggaagtg tcaacaacga attcttacaa tatatcaatc gggatcatgt ctatctcgtt
     4021 gccggattga atttgccact attgattgag ctagtatctc ggttggacgc tgaagtagag
     4081 acaactgttt tgatccagca ggtgctaact gattcaaaaa aaatgatcca gttttgtaac
     4141 gagcggctca atcagaaaat agaagaagac gaattttagg aggaagaaaa atgattgtat
     4201 tgacaagagt cgaccatcgg ttattacacg gacaagtagc tttttcatgg acacaaacaa
     4261 taggcgcaga ctgtattttg attgctaatg atgatgtacc tacgaatgag atacgaaaga
     4321 caacgatcaa attagccaag ccacaaggag tcaaactagt gatcaaatca attgatgact
     4381 cgattgccgc gttgaaaagc ggggtgacag ataaatataa gttattcatc gtagtggaat
     4441 cgatcgagga tgcatataag ctggcagaag cgtatccaga aatcaaaacc atcaacttag
     4501 gagggatcaa ggcaaaacct ggtactcgca gcatcggaaa agcggtaaat attttaccag
     4561 aagaggagga acgcttgaaa cgattaattg acaaacaaat agaagtagaa atccgtcaag
     4621 taccagcaga taaaaaagtg gatgtaagag aagttttgta agaaaggaag aacctcaatg
     4681 attgtacaag cagtcttatt aggaattgtt gcatttgtcg cacaatcaga atatgcacta
     4741 gggacatcgt tgctttctcg tccgattgtg acggggctgt ttacaggaat cgtgttaggt
     4801 gatgtaaaaa ctgggatcat tatgggcgca actttagagt tagcatttat cggatcgttc
     4861 tcagtaggtg cttcgattcc acctgatgtt gtgaccggtg ggattttagg gacggctttt
     4921 gctattacag ctggagccgg tacagaaacc gcgcttttac taggtttgcc gattgctacg
     4981 ttgacattga tattgaaaaa tatttactta gggcttttga ttccaattat gaaccataaa
     5041 gcagacaatt atgcagaaga aggaaattat aaaggagtcg agcatatgca cttgcttgcc
     5101 ggtatcggtc tttccttgat gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc
     5161 aatacgatcg gaaatctatt aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta
     5221 gctacagggt tgattccagc tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa
     5281 caagttgcac catatttttt cttaggtttt gctatagctt cttacttaga gattcctgtg
     5341 accggagtag ctatttttgg tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc
     5401 cgtcaactac cacttcaaac aactgcaggg gaggtagaag aagatgaaga attctgagat
     5461 caaaaacagc aaaataacga aaaaagagct aaatcacgta ttttggcgtt cttttcaaat
     5521 ggagttttct tggaattatg aacgtcagat gaatatggct tatgtttatg caatgattcc
     5581 gattttgaaa aagttatatc agtcgaaaga agaaatggca gcagctttga aacggcatct
     5641 agagtttttt aacacgaccc cacatatcgt tacactgatt ttaggaatca cggctgcaat
     5701 ggaggaagaa aataaggatg atcctgaatt tgatgtgact gcgatcgata gtatcaaaac
     5761 atctttgatg ggacctcttg cagggttagg agattctttc ttttggggaa ctttgcgatt
     5821 gattgccaca ggtgtgggta cctctttagc catgcaagga aatattttag gccctatctt
     5881 gttcattttg attttcaata ttccgcacat tctgtttcgc tatctagcaa caggttgggg
     5941 atatcgctta ggaacgggtt ttttgaaaaa gatccaggca aacggtatga tggaaagttt
     6001 gacattaggc gcgtctatta ttggactgat ggtcgttgga gcaatgactg ccacgatgat
     6061 caccatcaat attcctttaa aaatcggttc aggagaaaat gctgtgactg ttcaaagtat
     6121 ttttgatgat atcgttccaa atatcttatg tcttggagca tttggcatag tgttttatct
     6181 attgaagaaa gaagtgaaac cattaacgat cttgatcgga ctagctttct ttggaatttt
     6241 tggttcgtgg atagggattt tttaaaaaag gagcagagaa aatgtttacg atgcaagatt
     6301 atatctatga agaaaaggaa gtactaagta cgatcttgaa aaagaatgat ttttcgacaa
     6361 gagaacatat gaaaaaaaca gtgaatttac tgatcttagc aacgggttca tcttataacg
     6421 cttgtttagc agcgaagcca gcattggaat cttatggtga tcttactgtc gatattcaag
     6481 aaccttttta ttttttcaat tatggaaaac tttctccttc aatcgatact gtgattgctg
     6541 tgtcacaaag cgggaaaagt gcttctactg ttgaagcagt aaaaatgata caaaaacaag
     6601 ggttacctgt tgttgctatc acaaatgacg tgcaaagtcc acttgcttta gaagctgcac
     6661 aaattattga tttaggtgcc ggggtagaat cagtaggctt cgttacaaaa ggctattctg
     6721 cgactgtttt acagttacta ctcttaggga taggaattgg aataagtaaa aataaaatca
     6781 gcaaaaaaat agaacaagac tacatgcaac agttgcgcaa aataatcaac catttaccag
     6841 cgattattca aaaaacggaa gaattttttg atggatatca aagtttattt cgtttagcac
     6901 agcgtttcgt caccattgga tatggtccaa actggggaac agctaaagaa gcagagacga
     6961 aattgactga aacaatccgc gttccgtcac aaggatttga gttagaagct tatatgcacg
     7021 gcccatacct ggaggcagat gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa
     7081 ataaggaacg ttcgcaaaag ttgcaaagat atatgagtcg gtacgttgga gagactctca
     7141 ccattaccac aaaaaaagca agaaacgaga aaacacttgg attagcaata gaatgtgatg
     7201 aatatctgtc tgtattagcg cttgtcgttc cgtttcaact attcgcttat aaaaccgcag
     7261 tagctaaagg tattgacttg aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa
     7321 gtaaattata agaggtggaa atcatgctga aattcaatga agaagaacaa atcaaagcaa
     7381 taaaaggcgc actagcttta cgtccgcaag tagaagaaat catcgataaa ctttatgcgg
     7441 aaaaatttga cgctgtctat tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag
     7501 ctgtcactta tatgaatggg aaaagcaact tgcctgtctt tgtacagcat gctgcagaat
     7561 attacactac tggaaacaaa agattaacaa aagattctat cgttattttg tcctctgtta
     7621 ccggtacaac tcaagaagta gtgaaagcag tagaacaaat caagaaagta ggagcgacac
     7681 tgattggatt tatcgataaa gcgaacagta agttgtctca gctatgcgat tttgtggtca
     7741 cttatccagc tcctggaaca gaacaaatca aattttttat ggcagcagat cgattgatgt
     7801 atttgaatgg agaattcgaa gcttattcag aatactatga acagctcgaa cgttatttac
     7861 caacagggtt agtggaagca gagaaaaaag cagatgcatt tggcttatca tttgcagaaa
     7921 aacatcgacg tgatagtatg cattatttta ttggagcagg gaatcaatgg ggagctgttt
     7981 attcatatgc catgtgttac tgggaagaac aaagctggct tccatcgaaa tctatccatg
     8041 ctgccgagtt tcttcacggg acgttagaga tcgtagaaga aacaacccca gtcacattat
     8101 ttttaggcga agatgaacag agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa
     8161 tttgcagcaa ttatacattg atagacacaa aagactatcc agtagaaggt atcagtgaaa
     8221 aatatcgcgg acgtgttttg tctttcttat tgatgcatgt agtgacacaa cgcatcgatg
     8281 ctcatgtgga gcagttgaac tgccatccat tagagatacg ccgctactat cgtcagtttg
     8341 attattagga tagactggat aaaaagaata taaaaaataa agagagcagc gataactgct
     8401 ccctttattt ttttaaaata ttggcatcaa ttacgttata aaacacattt tttgcattat
     8461 ctacagttca acttataatc tgtttaatgg gacatgtggg tgccaaccat tttttgttat
     8521 gcaatagtac tatttagttg ttgcatggat ccgtagtaaa agtccaagta caacgagttg
     8581 cgccagttta catagcgttt tgtatccaga atgttgacta aaacaaaagt tcctatgtca
     8641 accgtcaaca gagacaatct gtccgttgca ggacctgaca gatttagaaa tgttcaatct
     8701 gtctcttatg tattttaaat caatactcac taggagaagt ttcctaacct cctaatagta
     8761 atgctggtag taacattgtc aaaactgctt cctcttagga acaagcaaag cataacgagt
     8821 gtaaaaataa aatgtagact attgtaacat atcattgatt atttcaagta aaaacgataa
     8881 aagaagtaaa caaacactta aaaacaatgg aaattagtcc caactttaag aaatgtatac
     8941 tataatggaa attatcaatt atttatttgg aggaatatcc atgaaacaaa aaatcgcagt
     9001 tcttggtcct ggatcttggg gcactgcttt agcacaaaca ttggcagaaa acgggcatga
     9061 tgtccgtatt tggggaaatg tacctgaaca gattgatgaa atcaataagt atcatacaaa
     9121 tcagcatttt ttacctgatt tgacgatccc agaatcgatt atcggatata aagaattggc
     9181 agaagcagtg gataacgcag atgctatttt atttgttgtt ccaacaaaag cgattcgttc
     9241 agtcgcacag gaacttattg gtaaaatgaa tactaagcca atcattatac atgcaagtaa
     9301 aggactagaa caagatacgc ataaacgaat ctcagaagtt ttggctgaag aaattccaga
     9361 agaaaaaaga caggcaattg ttgtattatc tggtcctagc catgctgagg aagtggcagt
     9421 acatgatatc acaacaatta cagctgcaag tattgatcaa aaagcagcag cctatgtaca
     9481 aaaactgttc atgaatgatt atttcaggat ttatacaaat aatgatgtga ttggcgtaga
     9541 aactggtgca gcactgaaaa atatcattgc aattggtgcc ggcgctatcc atggattagg
     9601 attcggagat gatgcaaaag cagcaatcat gactcgagga ttggcagaaa tcagccgtct
     9661 tggtgtcgcg atgggtgcca atcctctgac atttatcggt ttgagtggtg tgggggactt
     9721 gatcgttact tgtacaagtg ttcattctcg caattggcgt gccggtaact tgttaggaca
     9781 gggacataag ctggaagaag ttctagaaaa catgggaatg gtcgttgagg gtgtggcaac
     9841 gacaaaagca gcagtagagc tagctcaaca gctaggtgtt gagatgccaa ttactcaaac
     9901 aatctataat gttttgtata atggagaaga tatcaaaaaa gcagcaaaag aaatcatgtt
     9961 acgtgacgga aaaatggaga atgaattttc tcttcgttaa atagatgatc aatagttaat
    10021 c
//
LOCUS       DAZUMT010000102         9922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000102 DAZUMT010000000
VERSION     DAZUMT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.104"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..595)
                     /locus_tag="ACG8FM_002352"
     CDS             complement(83..595)
                     /locus_tag="ACG8FM_002352"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS5094979.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(609..2906)
                     /gene="recJ"
                     /locus_tag="ACG8FM_002353"
     CDS             complement(609..2906)
                     /gene="recJ"
                     /locus_tag="ACG8FM_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS5094980.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3048..3491)
                     /locus_tag="ACG8FM_002354"
     CDS             complement(3048..3491)
                     /locus_tag="ACG8FM_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS5094981.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3492..4280)
                     /locus_tag="ACG8FM_002355"
     CDS             complement(3492..4280)
                     /locus_tag="ACG8FM_002355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5094982.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4277..5218)
                     /gene="rnz"
                     /locus_tag="ACG8FM_002356"
     CDS             complement(4277..5218)
                     /gene="rnz"
                     /locus_tag="ACG8FM_002356"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS5094983.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5395..5982)
                     /locus_tag="ACG8FM_002357"
     CDS             complement(5395..5982)
                     /locus_tag="ACG8FM_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS5094984.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6203..7516)
                     /gene="obgE"
                     /locus_tag="ACG8FM_002358"
     CDS             complement(6203..7516)
                     /gene="obgE"
                     /locus_tag="ACG8FM_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS5094985.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7804..8814
                     /gene="gap"
                     /locus_tag="ACG8FM_002359"
     CDS             7804..8814
                     /gene="gap"
                     /locus_tag="ACG8FM_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5094986.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8899..9834)
                     /locus_tag="ACG8FM_002360"
     CDS             complement(8899..9834)
                     /locus_tag="ACG8FM_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5094987.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2729 a         2271 c         1716 g         3206 t
ORIGIN      
        1 tcagatagag aatctgactt ttgagggtcg ctttaaaatc gcttctttaa gaataacatc
       61 aagaatagct tcttttttct gcttaatatt ccattaatgt aatgatgtca tatcctttga
      121 ttttgtcgcg tccatggaga tccatcaatt cgatcaaaaa tgcgcagcct acgactacac
      181 cgcctaattg ttcgatcagt tcgatcgtcg ctttgattgt tcctccagta gctagcaaat
      241 cgtcacaaat cagcacacgt tgacctggtt taatggcatc cttatgcaat gtcaatgtat
      301 ctgttccata ttctaatcca taagtcactt cgatggtttc tcgaggtaac ttcccttttt
      361 tgcggactgg ggcaaaaccg actcctaact cataagcaac aggacatcct acgatgaatc
      421 cacgggcttc tggcccaact accatatcaa ttcgtttttc ttttgcataa tttacgattt
      481 gtttagtagc ttcccgataa gcgtcaccat ctgccatcag tggggaaata tctcgaaaca
      541 caattccttt tgacggatag tcgggaatac tggcaatata atcttttaaa tccactcttt
      601 gtttcctcct aggttgttaa ccatttcttt atggtaggaa tatcgctcaa aagcaaaaat
      661 tcctcaactt tgattttatt gagccgtcgc tggtataatc tgctctcagt caaaggatga
      721 ctttctgggc tagcgacttt ttgtaaaaca ccgttctcta ttgtaacaaa tttcaattca
      781 aaaaacacct gtatcataaa aattaataat ttttcaggga tacgcaaata ttgagctatt
      841 gcttttattt tatgacgtat atcaattcgt tcttgagaat ggatcaattt aaacaatttt
      901 gcgtactgtt ctcttgtgcc taccccattc aaataggcct catcaggtga aatccccatc
      961 agataaacac gattgaatga ataagaccga aaaacttctt tcagatccgc taaatcatct
     1021 ggacaatcca taacaactag agagtttggt tttctttttt caattattgc ctttacagaa
     1081 tctagtcctt cgaaaacaat cgcagcttcc tggataatct caggcacgaa tttcaacgtc
     1141 gaagaatcaa atgctaaata aaggctacct tccgacgttc ggctctgttc acggaaacgt
     1201 tttgcccgcc aatcaaatac ttgcagacca cccacagaaa aatcagatac catcagctgc
     1261 ggtttcttac gcccgttcca ttcattgatc gaaagctttc ctaccacatc tacctgatta
     1321 ttcagcagtt cttcttcttg agtaccaaaa ccaaaagcga ccgcatcaag ctgactcgat
     1381 gcatcggaaa ggacaaattt cagatgctgt tggtttgctc cgattctttt gatattttct
     1441 gcttggactt gtcgaaataa aaactgaggc aaaggattat ccgtaccaaa tggcgccaat
     1501 agtttcagct catcaattcg ctctactgtt acttcatttg gcagcaatac ttcatcaatt
     1561 ctcaactcag gccctctcat caagtcaatt tgatgatcga ctatatattg gttcatcttc
     1621 tcctgtagta tagtgacatt ttcacttggc atcgttaaac cgactgctgc atgatgtcct
     1681 ccgaagtaag taaataaatc tcgcatctgg tcaagcattt caaaaagatt caaggcttca
     1741 atactacgtc cagatccttt agctgatcca tcttctttta aagtaaggac aagcgttggt
     1801 ttacccgttt cattcattat tttcccagca acaatgccca aaacgccttc atgccatcct
     1861 cggtttacca atagatggat atgattctcc ttattgacca tcgctaatgc ttcttgtgtt
     1921 atctgctcta cgattgcttt tctttcttca ttgatctcat tcaatttttt tgcttgaacg
     1981 gatgcttcct cttcatcaaa cgtcgcaagt aacgagacgg caggacgtgg atcgcccatc
     2041 cgaccgattg catttagtct gggagcaatc gaaaaaccga tcgttgtttc gtctgcgtcc
     2101 ctcattttta cgccgctttc ttcaaatagc gcctgaagtc ctatccgttc tgaatgatga
     2161 atggcatcga ttcctaatga aacaaggatt cgattctcat cagtcaatga aactaaatcg
     2221 gcgatcgttc ctatcgctac taaatccaaa aattctgcag gcggttcttc caataatgca
     2281 gaagctactt tgaatgctac tccgactcct gccaaatctg gaaatggata gtgccccgct
     2341 ggatgacgag ggtgaacaat cgcataagcg tctggtaaat tctcaggtag ttcgtgatga
     2401 tctgtaatga tcacgtctac accagcttgc tgagcgtatg ctaccgcatc atttcctgct
     2461 accccgttat caacagtgat gatcaactgg attccttctt cgatcttttc ttggtatacc
     2521 gattggtttg gtccataccc atgctcaaat cgatttggca ggaatacttc tacttctgca
     2581 ccaagtaatt ccagtgtttc tttcattacc gttgcactgg tgatcccatc tgcgtcataa
     2641 tcaccgtaaa cgaggatttt ttctccactg attacagctt cttggattcg gctgattgct
     2701 ttatccatat cgtgtatcag atacggatca tgtaattctt gttcctttgg atgtaaaaat
     2761 cggttgatat ccttttcctc atggtaacct ctttgccaca gtaacttacc gacaaatgga
     2821 gataatttca gttcacggat catttggaga aaggattcag aaggctctgt ctcgcttaac
     2881 ttccaatcaa aatttgcttg tctcactttc tcactcctaa cttagttatt atagcaaagg
     2941 aaatcagcaa aaaaaagcag aaagaaagaa taaaaacaga tcagatccgc tagacagatg
     3001 ttcagcagga cctgatcttt tgtacgacca tttatgaaat atccctatta gtcaaaataa
     3061 ttgaagcttt gtttgggcga ttgagttttt tgttcttccg gcgctttcaa ttgactgatt
     3121 tgtgcggttt tcgcttgtaa ctccgcctga taagcagctt ccaactccgc acgttcgttt
     3181 ctaaactcgc gttgctgttc ttcttttgct tcttcaattt tcttttgaag ttctgtttga
     3241 tattctgaca tttcctgagt cagctgtttg atttgctttt tttgctgcca tagtgttgcc
     3301 gaagcagtca aaaatacaat cagtgcacca atcagcgcag aaccgatgat cactaagatc
     3361 aatggcgcag aaaaggaagt gaagccaaaa cttacaggaa catttttatt gttcaataca
     3421 gcaaaaagaa caataatcaa aactaaaaca aatccaagaa tcacattgct ttgctttttc
     3481 aattccttca cctacttttt gttaaaaatt ccgctggcta aataatcacc aatcgttgga
     3541 aacagcgtat aaaattttga tgcaacttct aaaataaacg gttgattgat ttcccgtttt
     3601 ggatgtctca tattgcggac gatcattttt gccaacttgg caggttctaa aacgatttgt
     3661 ccgacttttt ccaaatagct tccgcttgga tcagccttat cgaagaaatt tgtctcgatt
     3721 ggacctggat tcactgtcgt aacagctacg ccaagtggtt tcaattctaa tcgcaaagcg
     3781 ttggagaagc caaggactgc aaatttcgtg gcagaataaa ccgttgattt tgcagttgcc
     3841 atttttccag ccatagaggc gatattaatg atatgacctt gtcttctttc tgccatatcg
     3901 atcgcaaatt tttgtgtcaa aaccatcatt cctaagatgt tgacttcaaa catatcgtat
     3961 gcttttccaa ggtcgaatgt aaggaagtct tcgaaaatcc caaaacccgc attgtttacc
     4021 agaatatcga cgcgcccgac ttcttccatc accttttcat ataattcgtc cacgctttca
     4081 ggattgctga cgtctacctt gaaagcataa gcctctttgc cgctcaagcg tctgcactcg
     4141 tctctgacac cttctacaaa tgccattctt cgtgcgcaac ttataacgat tgctccttgc
     4201 tttgctgctt cataacagat ttgtgcgccc agaccgcctg aactacctgt tactaaaaca
     4261 actttatctt ctaaattcat gcctcttcct cctcgcgaaa tggaatctca atgctatcaa
     4321 aatctttcat aatcgtcgtg ttttcaaaca cttcttgtgc ttctttttca agctctaaag
     4381 ctgcttttcc taaatatctc gcactgatat gagtaagtag cagccgtttt gcctgcgctt
     4441 cttttgcaac ttcagcagct tgatgagtcg tggaatggaa atagttgtgc gccattcttg
     4501 cttcatcttt attaaatgtg ctttcatgta ccagaatatc gctattttcc gcaagcacaa
     4561 cggaattatg tgtttttctc gtatcgccta aaatagtcac gattcgtcct tttttatctg
     4621 gacctacgaa atcttttcca ttgatcgtcc gcccatcttc tagttggatc gtttcacctt
     4681 gcttcagttt gccgtacagt ggtccagctg gaatacctaa ttcttttaga cgatccacct
     4741 gcaattctcc tttatgatca tgttcaacca cacgataacc aaaactgtcg atcccatgat
     4801 ttagtttttt tgtataaaca gaaaattgct gatcagtaaa aatcggggct gtttcattta
     4861 gctcaatgaa tttcagcgga taacttaaac gcgtttgcga aatacctaaa gagactttta
     4921 tgtattcctc tatccctttt ggcccgtaaa tctctaatgg cgtatcccca ccttgaaatg
     4981 accggctgct gatgagcccg ggaagtccaa aaatatgatc gccgtgtaaa tgtgtaatga
     5041 aaattttacc gatttttctc ggacgaatag tcgttcgcaa aatctgcatt tgcgtgcctt
     5101 cgccacagtc gaacaaccaa acttcattgc gctcgtctaa aagtttcaat gctatacttg
     5161 tcacatttct atgttttgca ggaacacctg cacctgttcc taaaaattgt aattccataa
     5221 tctacctgac tttctttaag ttcctcttta attttagggt aaaaagaagg gataatcaac
     5281 cgtaagtcga gaatgggttc aaattaaaaa taacgttcta aagcgatagc gttttctttg
     5341 aaaattacta taaaaacagc agcgaacccg tttgagatcc tctgctgttt tttattaatt
     5401 tgaagatcca gaactttcat tgtcgttgga agtttgcgga tgtttgtcct tccaagtctt
     5461 ttgtgcagct tctttcgcat gattttcagc ttctttcgca tcatgcacat ctttttcgac
     5521 atcttcataa tccaagcgag taatttctcc gcctaattcc atcattacca attgattcag
     5581 tgggcgattg tcctcttcat cagcaatcaa gatcagtcct gtagctcctt caccgatttt
     5641 atttcctaca aagtcgaaca cggtctgggc atcttttatt tctttggcat cttgtgtcgc
     5701 accgatcatt cctccggcaa accatcctaa taaaatccct agcggaccac ctaatacacc
     5761 aaccaacatt ccgattaatc cacccgtaga agatttgtta ttaccggtaa agtcgataaa
     5821 atcttctatt ttgaattgat gtgtcccatt aggatcatgg ctgacaactg ccatctgttc
     5881 tcctttaatc gaacgaacag catgtaactt tttgatttct gaaaatgcct gatatgcctg
     5941 gctttctact tgaaaattta aaatgattac tcgtttgctc attaaaaacg cccctttcat
     6001 accttcttgt tatttatttt acctttaatt gaaaaataag aaaagaaaag cactcttttt
     6061 ttccaataaa atagggctgt gacaaaagtc gtatcacagc tcctttttcc gaataaacgg
     6121 cgttcttgaa gctgatcact tctgtcacaa cctcccttaa atctgaatct atattttttg
     6181 gtatttgttt ttatttgctt gcctattcga caaattcaaa ttcaaactca ccgatacgta
     6241 caaggtcgcc atctttcgca ccgcgtgccc gaagagcttc atccacaccc ataccacgta
     6301 attgacgagc aaaacgcatc actgtttcat catgatcgaa attcgtcatt tggaataatt
     6361 tttcgatctt ctcaccagat aagatccacg ttgcatctga atcacgatcg atttggaact
     6421 caggaccttc tggattgaat ccatagtgta cagtttcttc ttccagttct tcttcgtaca
     6481 atgggaattc tggcgttact tctaacaaat ctgctgtagc attcaataac gcgtccaatc
     6541 cttgtcgagt cactccagaa attggaaata ctgggatgtc atcagcaaat tcatcttctt
     6601 tttccttgtt cagctgttct ttgaatttca ctagattttc cggtgcatcc ggcatatcca
     6661 ttttgtttgc cacgatgatt tgcggacgct ctagcaagcg taagttataa gtagataatt
     6721 ctttgttgat ggcaagatag tcttcataag gatctcgtcc ttccatccca ctcatatcga
     6781 tcacatgtaa aattactcgt gttctttcga tatgacgaag gaattgtgta cccagcccaa
     6841 caccttgcga agctccttca atcaagcctg gcaagtctgc tgcggcaaaa cttctgccat
     6901 cacttgtagt aaccattcct agattaggta cgagcgtcgt gaaatgataa gcgccgattt
     6961 ttggacgagc agatgaaata acggatagta aagtcgattt cccgacagat ggaaaaccta
     7021 caagtccaac gtctgccaat actttcaatt ctagttcgat ttttcgctct tgtcctggtt
     7081 caccgttttc agcaatttcc ggtgcaggat tccgaggaga ggcaaaacgg atattccctc
     7141 gtccaccgcg tccacctttt gccacaacta gtgtttgacc attttcaatt aaatctccta
     7201 aaagagcacc agtctccgca tctcggacag tggttccttg tggtacttta acataggtat
     7261 cttctgaacc acgaccgtgc atccctttac tcatcccatt ttcacctggc tgcgctttga
     7321 aatggcgatt gaaccgaaaa tccatcaatg tacgtaaccc ttcatcgacg atcaagatga
     7381 catcgccgcc acgaccaccg tctccgccag ccggtccgcc gtcaggaacg tatttttcgc
     7441 gacgaaatgc aaccattccg tctccgccct tgccagcttt cacatcgatc gttacttgat
     7501 ctaaaaacat ggacataatt aagtcctcct attattcatt tatttatttg actgcctgaa
     7561 tttccacaat aaacaaaagt cttacttatt ttatcggtga aatctgattt tgtctatcat
     7621 ttcagcaaaa agattgttta ccttcttctt tttttatcat ttggtaaggt ttccaacctt
     7681 ttttctgcta ccaagtataa ttttttgaca aaatattctt tgcgtagcag aataacagtg
     7741 tattaattgt attaattgag gatgccgttt cacagcaccc ctaataaagg aggaactttt
     7801 gatatgacag taaaagtagg tattaacgga tttgggcgta ttggccgtct ggctttccgc
     7861 cgcatcaaag aagtctcaga tgatattgaa gtagtagcaa tcaacgactt aacaagtcca
     7921 actatgttag cacaattatt gcaatttgac tctactcatg gtacttatcc tggcacagtt
     7981 actgcaacag aagactcaat cgtagtggat ggcgaagcaa ctcgtgttta tgcagaaccc
     8041 gacgcaagca aaatcccttg ggcaaaagaa aacggtgtag atatcgtact agaatgtact
     8101 ggtttttata catctgaaga aaaagcaaaa gcgcatttag atgctggtgt caaacgagta
     8161 gttatttctg ccccagctgg tgctatgaaa accatcgtct ataacgtcaa tgatgataca
     8221 ttagatgaaa acgacaaaat catctcagca ggttcatgta caacaaactg tttagcacct
     8281 atggcttatt tcttaaataa tgaatttggg atcgaagttg gtacaatgac aacagttcat
     8341 gcctacactt ccacacaaat gctcttagat ggacctgtaa aaggcggtaa tttgcgtgct
     8401 gctcgttcag ctgctgacaa tactatccca cattctactg gtgctgcgaa agcaatcggc
     8461 ttagttatcc ctgagctaca aggaaaatta caaggacacg cacaacgtgt tccagtcgtt
     8521 gatggttcat taactgaatt ggtttcagta ttgaaaacaa aagtaactgc tgatcaagta
     8581 aacgaagcaa tcaaaaaaca cacagttgac aatccttcat tcgggtatga tgaccgtcaa
     8641 atcgtttcta gcgatgtcat tggaacaact caaggttcga tctttgaccc aacgcaaaca
     8701 gaagtaacaa ctgctggtga tttccaactt gttaaaactg ttgcatggta tgacaatgag
     8761 tatggattta cttgccaaat gattcgctta ctagaaaaat tcgctaactt gtaatttgat
     8821 agatcaatcg ataaaagaag cctttgactg gatccagtca aaggcttctt tcttttcatc
     8881 ccctactttt aaactcgttc aatgaacata ttggcttaaa aattgttgga ctacttgctg
     8941 atatttttca ggatctgtct tgtatgcttc cgcatgctcg gctcctttta caacatactt
     9001 ttcttttggt cccttcgttg cacgatagac cttatctaac atttcaaatg gtacaaatgt
     9061 atcttcgtct ccatgtataa agagcattgg gcgctggttt ttcttcaatt gttccactgc
     9121 atttgcttca ccgaaaaagt agccagcacg gacttttgtc actagactcg ttacttgcat
     9181 aagcggaaat gctggaagac taaacatatc gtctagctga taagcaagct catcatgaac
     9241 ggaagaataa ccgcaatctt caatgattgc tttcacatta tctggtagtt tttctccgct
     9301 agtcatcata actgttgctg ctcccatgct gacaccgtat agaacgattt cttgcgattt
     9361 tccattgttt tctagcactt tgtttatcca ctgtacatag tctttacgct ctggccatcc
     9421 aaatccaatg taatcccctt cgctttttcc atgtcctctt gcatcaggaa caaggacatt
     9481 gtaacccata tcatgaaaca tctttgcgta attggtcatc gtttcagcgt ttcccatata
     9541 gccatgagcc acaagagctg ttttttcact tttcgtttca gcaggaagat aaatcgctga
     9601 taatttcaga ccgtcttttg aagtgatggt ccaatcgctt cggttttctt ttgaactaaa
     9661 ccatttatgt gcatccgttt ctttggatgt ctgtgttgta ccaggtgtat cttcgtctaa
     9721 aaagtctttc tcagaaggca caacagcata attataaaaa taattccctg caaacactaa
     9781 cgcaacaatc agagctaaag cgacgcctaa aaggattttc atccaaagtt tcataaatca
     9841 tccatctcat ttctcttttt tcatataacc ttgaattagt ataatcaaag aatctctact
     9901 atgcaatgtt catttttata ac
//
LOCUS       DAZUMT010000103         9891 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000103 DAZUMT010000000
VERSION     DAZUMT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.105"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(278..1126)
                     /locus_tag="ACG8FM_002361"
     CDS             complement(278..1126)
                     /locus_tag="ACG8FM_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5094988.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1290..2192
                     /gene="gnd"
                     /locus_tag="ACG8FM_002362"
     CDS             1290..2192
                     /gene="gnd"
                     /locus_tag="ACG8FM_002362"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS5094989.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2194..3195
                     /locus_tag="ACG8FM_002363"
     CDS             2194..3195
                     /locus_tag="ACG8FM_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS5094990.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFNVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMCEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3192..3476
                     /locus_tag="ACG8FM_002364"
     CDS             3192..3476
                     /locus_tag="ACG8FM_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS5094991.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3509..4306
                     /locus_tag="ACG8FM_002365"
     CDS             3509..4306
                     /locus_tag="ACG8FM_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5094992.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLSSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4320..5153
                     /locus_tag="ACG8FM_002366"
     CDS             4320..5153
                     /locus_tag="ACG8FM_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5094993.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5171..6028
                     /locus_tag="ACG8FM_002367"
     CDS             5171..6028
                     /locus_tag="ACG8FM_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5094994.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6050..6559
                     /locus_tag="ACG8FM_002368"
     CDS             6050..6559
                     /locus_tag="ACG8FM_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5094995.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6596..6997
                     /locus_tag="ACG8FM_002369"
     CDS             6596..6997
                     /locus_tag="ACG8FM_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094996.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7109..7462
                     /locus_tag="ACG8FM_002370"
     CDS             7109..7462
                     /locus_tag="ACG8FM_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5094997.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7496..7939
                     /locus_tag="ACG8FM_002371"
     CDS             7496..7939
                     /locus_tag="ACG8FM_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5094998.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7956..9041
                     /gene="uxuA"
                     /locus_tag="ACG8FM_002372"
     CDS             7956..9041
                     /gene="uxuA"
                     /locus_tag="ACG8FM_002372"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5094999.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9034..9747
                     /locus_tag="ACG8FM_002373"
     CDS             9034..9747
                     /locus_tag="ACG8FM_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5095000.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3302 a         1559 c         2171 g         2859 t
ORIGIN      
        1 accaaaattt tttttaattt tttagaaaat aagaaacaaa aataataaaa aaaagaaaag
       61 ataaattttt tactactgta tactaacttt gttatcgcat ctaacaagta actcgaataa
      121 taccaataca accttctcca aagatgcgat gacatacgtt ctaacccacc gactattgat
      181 gttctgtacc acccttgcag aaccaaaaaa gccactacca aatatttggt agtggctttt
      241 tttgttttat ggatttttcg ctttttctgt tatggtgcta ttctttgatt tcagaaatag
      301 tatgtcgaac tttagcgata gaacgacgtg attctttttc gttgtaaaac atcaaaatca
      361 catatagaga gtccaaaatg cttagttgag aaatacggga agctaaagct tcagatcgga
      421 attctgtttc ttcagaaata gaaataaaaa cgacatcgcc aagtttagca agggaagaat
      481 tagcttggct agtaataacg attgtttttg ctccagtttt tcttacggtt tcagcaatat
      541 ggattgtttc ttttgattgc cctgtgtgag aaatcaaaac agcacagtct ttttccgtca
      601 ataaagaagc ttccatcaat tgaatatggt aatccgtgct gtgagaagtt gcgatagggg
      661 aacgcaaaaa tttatgataa gcgtcagttg ctacaatctc tgaaccgcca acaccgaaaa
      721 aatagacacg tttagattca ttgataatag tagccgcttt ttttagatcc ttttgttgta
      781 acaacttttt tgtgtcattt aaagttgaaa tactagactc gaacaccttt tgcgccatag
      841 tgagctcagt atctgttttc tgaatatttt cgtggataga aatagcagat agatcatttt
      901 cttggatcaa catggccatt ttaaaatctt taaatccatt ataaccaagt ttttttgtaa
      961 attgaaaaaa agtagagtca gcaatgccca gttcacttgc taaattgcta ataggactat
     1021 gacttacttt gcttggattt tctaaaatat aatcggcgat agcctgttct tttgggctga
     1081 agtcgttgta cattgttttg atttgtagtc ttatggagac gctcatgaga attcctcatt
     1141 ttcataataa gattatcatt ttttccgttc tcattaatgt taaagataca aggagagtag
     1201 gaaaaaatca aacaaaaaat atttttgtaa aatctattta caaaaaaatt tttttgtata
     1261 atgagggtat aagaaatgga ggcgctatca tggaaattgg tgtaattggt ttgggaaaaa
     1321 tgggattgaa tttagcttta aacttaaaag agcaccatca caaagtacaa ggactcgaca
     1381 tttcaagtac agctgttagt caggcaagag aaaatgacat tcctgcttat caagagatct
     1441 ctgaatttct agctactttt gagcagaaaa aaatcatctg gttaatgctt cctgctggag
     1501 agataaccga aaacatgtta agaaaacttt ttccaaaact cggaccagga gacattatta
     1561 ttgaaggcgg aaactcttat tataaagatt caatccgtcg agcagaagag tttcagaaaa
     1621 atgaaatcgg ttacttggat tgtgggactt ctggaggaat cgaaggagca cgacatcaag
     1681 cctgcttaat gattggagga gataaagaaa cttttctatc cgtggaacaa ttattcaaag
     1741 atgtagcaat tgaaagagga tatctgtatg ctggtgcatc cggaagcgga cacttcttga
     1801 aaatgatcca taatggaatc gaatatggaa tgatgcaagc aatcggtgaa ggattccaac
     1861 ttgtacaaaa aagtgagtat gcttttgact tggaacaagt cgcacgtgtt tggaatcatg
     1921 gttcggtgat tcgcggctgg ctgatggaaa tcatggaaga tcagttaaac gaacatccca
     1981 atttaagtaa ttatcgggga atcgtttctg catcaggtga agcaaaatgg acggttgaaa
     2041 cagcgctaga aatggctgtt ccagttccag taattgcaac tagtttgttc atgaggaatc
     2101 tttcacagga agaagatagt ttttcagcaa aagtagtttc agcactaaga aatgggtttg
     2161 gcggacatga aatagttgaa aagggtgagt aaaatgccag attatcatat agcaatcgtt
     2221 aattccagca gtttcggaag agtctttcca caccacttgg aaagattaaa gtcagttgga
     2281 gacgttcatt tctttaatgt tgatcaagag attccaggaa aagaactagc ggagttactt
     2341 aaaggattca atatcattat tgcaagtgtt acgccatttt ttcgacgaga tttttttgaa
     2401 aacaaagatg aactgctatt gatttcccga catggtattg gttacaacaa tattgattta
     2461 aaagcagcaa aagaacacca gacaatcgta tctatcatac ctgctttagt tgaacgaaat
     2521 gctgtagcag aaaataatat aactaatttg ttgtccatgc ttcgacgcac gacacaagca
     2581 caagcaagag tcaaggacga tcgatgggaa gatcgggcgg agttcgtcgg taggacgctg
     2641 tttaacaaaa cagttggtgt gattggtgca ggaaataccg gaagcggagt aatcgaaatt
     2701 ttgcgtaacg gttttcgctg tgatgttcta gcttatgatc cttacaaatc agatttatac
     2761 atcgaaagct ttggtgcaaa aaaagtagaa cttcaagaat tactggaaca atcagatatc
     2821 atttgtttat gtgcgaattt gacagaagag aactatcaca tgatttctga taaagaagtc
     2881 gatcagatga aagaaaatgt ttatctatct aatagtgcaa gaggcgctct agttgatgaa
     2941 gaagcgatga ttaatggtct gaaaagcgga aagatcgcag gatatgccac agatgtttta
     3001 gaagaagaac cgggacgtca agatcatccg tatttagcgt ttgaacaagt agtgattacg
     3061 ccacatatat cggcctacac aagggaatgt ttagaagcaa tgtgtgaaaa atgtgtcaga
     3121 gatgtcgaac agatcagtca gcaccttctt cctgaacgtt ctgtccagac aaagagcaac
     3181 tatataaaag gatgatgaag aaaatggtga acggtttaaa agtgaaaaca ggccctcaat
     3241 tttatttata tgaagaaggt ggtatttcaa aagtttctga tctcttgaaa tcatacggcg
     3301 cgaaaagagt actggtcacc cacggaacgg tatcatggga aaaggcgcta cctaaattag
     3361 tatttttgaa tgacgaaacg attcaatttt tttatcatcg ttattctgga gaatgtagtt
     3421 atgctgaagc cagacgaata gctacaatca tcaaaaaaat gaaatcgatt tcgtagttgg
     3481 cgttggcgga ggaaaactga ccgatctagt gggttatagt gccaatcttg caagaatcga
     3541 tttcggagtc atccctactt tagctagtaa ttgtgctcca tggacacctc ttgctgtcat
     3601 gtataaggag aatggacaag cagaagggaa aacagaacac ttttacagac aagctgcttt
     3661 ttttataaca gatccccaat tagtgattga ttcccctgtt ccatatttca ttgccgggtt
     3721 agcagataca ctagcaaaat ggtatgaatc tgagctcatc ttagaacaag attttttgca
     3781 agatgaatca tttttgaagc tagcacaaga tactgcgaag atctgcaaag aagagatttt
     3841 gaatcattct gctaaagcaa tccaagatat ggcacaaaga aaactgtcat cggagttcaa
     3901 acagctttca gagattatct ttgctgttgc tggtttagtt ggaggattag gagataaata
     3961 cgcaagaaat gtggcagcac atgctatgca tgacgctatc agtaaatata ttccggaaag
     4021 ccatcgtttt ctacatggag aaaaagtagc gtatgggatt ttctatcaac tggcactaga
     4081 aggtcgctgg gaaaaaatag acgagttata tcctttgtat cgggaattag aactgccggt
     4141 ttctctagaa caaatgggat tatttccgaa agataaacag ataatagata aaatggctga
     4201 atttattgat tcgaaagaga aagtgcattt gattcctata gaggtaacac agaaatcact
     4261 gatacagtca atgatggaac tggaaggtta tatcagaaag atctgaagga ggaggacaca
     4321 tggaaacatt aagtgtagta caaagtttgt tgattgcttt atgggtagcc gcggttatgt
     4381 cccgctggtt aggtggagga gccacgttga ccttacgttt ttctcctttg atgactggat
     4441 tagtcgtcgg gttgatcatg ggggaagttg cacaagcgat gattgttaca gccgctttgc
     4501 aaatgattta tatgggggtt ttttctccag gaggatcaat gccagctgaa ccatctattg
     4561 cagcagctat cgcagtacca gtcgcattat taggggatct gtcgccagaa gcagcgattg
     4621 ccgttgcggt gccagtcgga ttgctcggaa gttacttgta tcagtttcgt tttttcatca
     4681 atactttttt aggtaaatat acagacaaag ctgtagaaga tttgaataca aaagctatgg
     4741 cccgatcaat tattctttat ccaacggttg cctcattttt attattcgtt ccgcttgttt
     4801 tcattgcttt atatttaggg gcgccattga tcgctgatgt aatcaaagct ttagagggaa
     4861 cagtcgttat ccacattctt gaagtagtag gtggagggtt agcggcaatc ggtatcgcga
     4921 caactgtata cgttatcggt cgaaaagatt atctggtctt tttcttcctt gcttatttta
     4981 tgagtgttgt tttgaaatct ttagatatta cgatggttac ttatgccatt tttgggatta
     5041 ttatcgcttt gatctttgtc cagtcgcaaa aagggaaaca agtacagcct gccacaagca
     5101 ataacaattc agcatctttt gatgacgaag atgactatga tgacggattt taatttagga
     5161 ggaatgggaa atggctgaaa taatggataa tcaaacaggt gctcctgaag aaatcacgaa
     5221 aaaagatgta acaaaagcct atctgagatg gcattttgca aacgaaattc ctcactcttt
     5281 tgaacgttac ttggcgccat cactactata cggaatgatg ccaatcttga aaaaacttta
     5341 taaagatgaa gactctttaa aagcagcata taaacggcag ttactgttct tcaacactca
     5401 attaagctgg ggtggcggtg tgattactgg cctaatgtct tctatggaac agcaaagagc
     5461 agaagaagag tatgaagaaa aagagatctt gatgcaggat gacttgatgt ataacacgaa
     5521 agcgggattg atgggggcac tagcaggaat cggagatgca atcgattcag gaaccgtcca
     5581 atatattttt atcgcaatcg cggtaccttg ggcacaacag ggaagtcctt taggcgcact
     5641 ttttcctttt atctgctttg ggttgtatca ggttttacta ggggtatttt tcgcaagaca
     5701 agctttcact atgggccgta atgctacagg cctaaagcaa aacactggtg ttcagacagc
     5761 gattgaaatg ctatcagttt tagggttgtt tatgatgggg gtgcttgcgg gaaactatgt
     5821 aaaagtagaa tcttcattga aatttgcgat atccggacgt gagtttgtta tccaggaaat
     5881 tcttgatcaa atcgtgccag ggatattacc tttagctgtc gtaatgggtg tgtacttgtt
     5941 ctatacgaaa aaaggattga aagtcacaca agcattatta tggctgactg gaatattaat
     6001 tgtgctagca ggaatcggta ttttataaaa agaagggaac gtgaataaga tgggtgtagt
     6061 caatttagcg cgtgtggatg agcgtttgat ccatgggcaa gtaatggtaa cgttatctca
     6121 aaaaagtgga gtgaattcca tttttgttgt agacgaagtt gtagcaaaag ataaatttat
     6181 gagagatctt tacaaaaatg caggaagccg tacaggacaa aaaacaattg tcattacacc
     6241 agagaaagct aaattctatt gggatgaata tcaatttaaa gaatacaatt gtattttgat
     6301 cgcaaaaaca gtccaaacga tttacgactt agtaaaacac ggaatcccca tggaagaatt
     6361 gaatattgga gggattgctc aaaaaaatcc tgatacagat cttttagtga cgaaatctgt
     6421 ttatttaaat aaatcagatg ctgaaaaatt gaaagaaatg aatgaacaat atggcgtaaa
     6481 gaatatttat tttcaagcca ctccatctgc acctaaaaca ggattaaaag atgtattgaa
     6541 caagtttgat ttgtcataaa caaatgctta gaaaaaagat aaagagggcg aaaaaatgaa
     6601 gaagaacgag ctgtttcgtg attgggaatt tcgttatcgc tatatttacc gtaagcgccg
     6661 tacaaaaaag agtaaacagc gttttctttc agcattagta tcagatattt acagtatgag
     6721 aacagatgtg acagtcattg cttacgatac gctagcctac cgatcaaaaa atatttatgt
     6781 tggagatatc gaaaaagcag agaaagttat ctgtacttat tacgatacac ccgttcatgc
     6841 attaggaagt tatttcatgt ttgattggaa agaccaaagg aaaaaaacaa tctactctat
     6901 attattgtcc ttcattttgt tgttcagttt aggttggtgg ggaatgatga tatataacaa
     6961 aaatcctcat cacgtattcg atctgctatc cgtataaaca ggtatcactg tactagcatt
     7021 tgggagctac tttttcttat tgggaaaagc agctcgcgga tggcctagca gacaaacttt
     7081 tattcgaaat acttcatcca tattaacgat gctagaaatg atccgtacga ttgatgatcc
     7141 gagtgtagcg tatgcgtttg ttgatgaagg ctgttatgga gaaaagggct tagacagcgt
     7201 gagatcgggt atgaaaaaag aggctattct tttttatcta gactctgttg gggcagatac
     7261 accgctacag ttttcgggaa attatttttc aaataaagaa cagtggctga aacaagttga
     7321 taagttgaaa gaaaaaaacg tcaattatat ttttagtgca agaaaaaagc aagcgcagtt
     7381 cttctatttg accaaaacag atttacgagg gaaaacattt aattggcaaa atgccaatca
     7441 gattattgct ctttttcgat aagtaaaaag tgcaaaacaa ggagtgagaa gactaatgtt
     7501 ggaaatcatg atcgttacac atggtaagtt gagcgaagga ctgaaagatg cggcggaagt
     7561 aattgtcggg acatcgagcc atattgaaac aatgtgctta gagtctgggg aggacatcca
     7621 aacattcgga gagaaggtca aagaaaaatt aactatgatc aacacagcag aacccatgct
     7681 agtatttgtt gatctaatta gcgcaagtcc gtacaatcaa gcgctcttgg cagtcaatga
     7741 actgactgaa gaaaagaaag cagccactta tgtcataggg ggcgtgaatc tgccaatgtt
     7801 gttagaagca atcaatcatc agatgcttca cacaccgata gaagaagcgg tggaacaaat
     7861 tgtcaaacaa ggaaaagata atttaactgt ttggcatgct tcagcggaag aaaaaaaatt
     7921 agatgaggac gatttttaga aggaaggctg aaaagatgaa gtggggattt agatggtatg
     7981 gtgaaaaagg ggattcgatc ccattaaaat atgttcgcca aataccggga ataaatggga
     8041 taattggaac tttgctgact aaaatgcctg gagatgtttg ggagatttca gaaattgagg
     8101 agttgaaagc atctgttgag agccaaggac tggaactgct tggaattgaa agtgtagcga
     8161 tccacgatgc aatcaaggca gggacagatg aacgagatta ctatattgat aactatatac
     8221 gaacaatcaa aaacttgtct cgatgtgggg ttcgattgat ttgttatagc ttcaaaccaa
     8281 tttttggatg ggcgaaaact gatttgttta atgaaaacaa cgatggaagc tattctcttt
     8341 tatacgatca gaaaatcgtc gatcagatgg agccaggaga aatgtatcaa ttgatacaca
     8401 gccagtcaaa gggattcaaa ttgccgggtt gggaagaaga acggttgaaa aaattcgctc
     8461 agctggaaaa actgtatgaa ggaatgaccg aagaagaatt atttgaaaat ctgacttatt
     8521 ttttagaaaa agtgattcca gtgtgcgaaa aagcagatgt aaaaatggct atccatccag
     8581 atgatccgcc atgggaactt tttggttttc ctagagtgac gaaaaatctt ggcgacttga
     8641 agaaaattct aaatgcagta gattctccat ataatggact aacgctgtgc acaggatctt
     8701 taggggcaaa tccggaaaac gatttagttg aaatcatcca tgaggtaggt tcgcgcatcc
     8761 attttgtcca cttcagaaac gtggagtttt taggagaaag aaagttcaaa gaaacggcac
     8821 atccctctac agaaggctcc cttgatatgt atcatattat gaaagcgttg gtgcaagaag
     8881 ggttcgacgg tcctatacgc cctgatcatg gtagaaccgt gtggggtgaa gtggctatgc
     8941 ctggatatgg cctgtatgat cgtgcaatgg gactcactta catgcaaggt ctatatgagg
     9001 cgataagtaa ggaaaaacca ctgggagagc attatgaata aaatcaagct cttcatcttt
     9061 gatatggatg gccttttatt agaaactgga cgtttagctt atcgtgctta tgtaaaatca
     9121 gcacaaaagt atgattatga aatgcgtaag gaagtctatt atttactaac tggccaaaca
     9181 gaaatggcga taagagaaca aatgggcatt ttgtacggag aagatgttcc atatatcaaa
     9241 tggcgagaag caatcaatca atataaagaa aaaatcgtga aagaagataa gagagtatat
     9301 acgaaaaaag gagcagaaga aattttatct tttgcaaaag aaagaggaat ccatacaatc
     9361 gtggcttcgt ctaatacacg ggtaaaagta gaaatgtatt taaaaatgga aaatctatat
     9421 gccttgtttg atcaaatcat ttcaggggat gatgtaaaaa aaggaaagcc ggaaccagag
     9481 atctttttga aagcttgttc aaaaatgaat attcctccaa gtgaagcact tgtatttgaa
     9541 gactcgattg cagggatcga agcagcaagg cgagcgggca ttttgtcttt tctcatcaaa
     9601 gatcatttga tagggttgcc cgatcataat gggaagcata agttgaaagt agatgtaagt
     9661 ttatatgaat ccaataaagc agactatgaa ttttatgacc tgcatcaagc aaaacgattt
     9721 ttagaaagta aagggttgta tctctaataa agaggactat tttctagtcc tctttattta
     9781 ttgtcctgca cgactgaggg aacagatgaa aaagaagtaa ttttttattt tgaagtatct
     9841 accttatttg tagtgaacaa tttagaacaa acacaaaacg catctactgt t
//
LOCUS       DAZUMT010000104         9870 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000104 DAZUMT010000000
VERSION     DAZUMT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.106"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..263)
                     /locus_tag="ACG8FM_002374"
     CDS             complement(18..263)
                     /locus_tag="ACG8FM_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS5095001.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(378..956)
                     /locus_tag="ACG8FM_002375"
     CDS             complement(378..956)
                     /locus_tag="ACG8FM_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5095002.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1033..1563)
                     /locus_tag="ACG8FM_002376"
     CDS             complement(1033..1563)
                     /locus_tag="ACG8FM_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS5095003.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="ACG8FM_002377"
     CDS             complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="ACG8FM_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS5095004.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2420..2815)
                     /locus_tag="ACG8FM_002378"
     CDS             complement(2420..2815)
                     /locus_tag="ACG8FM_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS5095005.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2812..4224)
                     /gene="cysS"
                     /locus_tag="ACG8FM_002379"
     CDS             complement(2812..4224)
                     /gene="cysS"
                     /locus_tag="ACG8FM_002379"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS5095006.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4221..4757)
                     /gene="epsC"
                     /locus_tag="ACG8FM_002380"
     CDS             complement(4221..4757)
                     /gene="epsC"
                     /locus_tag="ACG8FM_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS5095007.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4843..5082)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5079..6539)
                     /gene="gltX"
                     /locus_tag="ACG8FM_002381"
     CDS             complement(5079..6539)
                     /gene="gltX"
                     /locus_tag="ACG8FM_002381"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS5095008.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6709..7866)
                     /locus_tag="ACG8FM_002382"
     CDS             complement(6709..7866)
                     /locus_tag="ACG8FM_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS5095009.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7900..9270)
                     /gene="radA"
                     /locus_tag="ACG8FM_002383"
     CDS             complement(7900..9270)
                     /gene="radA"
                     /locus_tag="ACG8FM_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS5095010.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9333..9860)
                     /locus_tag="ACG8FM_002384"
     CDS             complement(9333..9860)
                     /locus_tag="ACG8FM_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS5095011.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2507 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc
      361 attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt
      421 tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg
      481 tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac
      541 tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaatcgc taaaggttct
      601 tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag
      661 acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt
      721 tgattgctta taatgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa
      781 gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa
      841 tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg
      901 gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa
      961 taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat
     1021 tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt
     1081 taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt
     1141 ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt
     1201 tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt
     1261 cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt
     1321 ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc
     1381 cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt
     1441 tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg
     1501 gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt
     1561 catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac
     1621 actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc
     1681 ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc
     1741 tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc
     1801 tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg
     1861 ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt
     1921 tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt
     1981 gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt
     2041 tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc
     2101 ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg
     2161 cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc
     2221 ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc
     2281 aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc
     2341 agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg
     2401 atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga
     2461 tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga
     2521 accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag
     2581 catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca
     2641 tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca
     2701 gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa
     2761 tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc
     2821 tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa
     2881 atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc
     2941 gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat
     3001 cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg
     3061 ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc
     3121 tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc
     3181 gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt
     3241 gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact
     3301 gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc
     3361 aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc
     3421 atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc
     3481 tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc
     3541 gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat
     3601 atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc
     3661 ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg
     3721 ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc
     3781 aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac
     3841 caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt
     3901 agcaggctcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc
     3961 aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc
     4021 cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt
     4081 atcgaaagca atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc
     4141 gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt
     4201 caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg
     4261 acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca
     4321 cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca
     4381 gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg
     4441 cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg
     4501 acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc
     4561 tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg
     4621 tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc
     4681 aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct
     4741 cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc
     4801 ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc
     4861 gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt
     4921 tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc
     4981 actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt
     5041 gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa
     5101 tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc
     5161 atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt
     5221 tctttttgaa cagctttgat ggcagctttg acagttggag catctactac ttccatttgt
     5281 tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct
     5341 tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt
     5401 tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt
     5461 gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg
     5521 tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt
     5581 ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat
     5641 tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag
     5701 ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt
     5761 gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt
     5821 ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg
     5881 gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca
     5941 acagcaaaat tataagttgg cataccatcg cgtttttgta tgacaaagtc gccaccgata
     6001 ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg
     6061 cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct
     6121 tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt
     6181 gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta
     6241 gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg
     6301 ccgtattctc caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt
     6361 tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga
     6421 ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca
     6481 tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt
     6541 ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct
     6601 tctcaaatat catttattta aagccactca gcacaaattt taccttttct aaggtaaata
     6661 gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg
     6721 tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt
     6781 agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg
     6841 ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc
     6901 tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg
     6961 gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg
     7021 gaattcagag actttattca agttatagtc attggttaca atgacaccat ctaaaagctt
     7081 agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat
     7141 ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc
     7201 acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa
     7261 attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc
     7321 gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg
     7381 ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga
     7441 accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc
     7501 taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta
     7561 aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa
     7621 gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga
     7681 gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt
     7741 ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg
     7801 taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt
     7861 ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa
     7921 cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc
     7981 taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg
     8041 ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca
     8101 ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa
     8161 atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt
     8221 ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa
     8281 atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg
     8341 gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc
     8401 accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg
     8461 ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg
     8521 caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg
     8581 ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat
     8641 ggctatccca tttgttttag cgattttgag tagttctgca gtggtttctc ttacttggct
     8701 gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat
     8761 aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc
     8821 gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc
     8881 tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta attgctgaga
     8941 aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga
     9001 ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg
     9061 aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa
     9121 acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca
     9181 ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca
     9241 aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag
     9301 acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct
     9361 tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg
     9421 ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac
     9481 atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg
     9541 tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat
     9601 tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt
     9661 tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag
     9721 cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca
     9781 gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact
     9841 tcaaatcctc tttttctcac cggttttcct
//
LOCUS       DAZUMT010000105         9551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000105 DAZUMT010000000
VERSION     DAZUMT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.107"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..417
                     /locus_tag="ACG8FM_002385"
                     /pseudo
     CDS             1..417
                     /locus_tag="ACG8FM_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(421..1140)
                     /locus_tag="ACG8FM_002386"
                     /pseudo
     CDS             complement(421..1140)
                     /locus_tag="ACG8FM_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1091..1300)
                     /locus_tag="ACG8FM_002387"
     CDS             complement(1091..1300)
                     /locus_tag="ACG8FM_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095012.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(1282..1350)
                     /locus_tag="ACG8FM_002388"
                     /pseudo
     CDS             complement(1282..1350)
                     /locus_tag="ACG8FM_002388"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            1577..2850
                     /locus_tag="ACG8FM_002389"
                     /pseudo
     CDS             1577..2850
                     /locus_tag="ACG8FM_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2957..3988)
                     /locus_tag="ACG8FM_002390"
     CDS             complement(2957..3988)
                     /locus_tag="ACG8FM_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095013.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3995..4825)
                     /locus_tag="ACG8FM_002391"
     CDS             complement(3995..4825)
                     /locus_tag="ACG8FM_002391"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5095014.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4822..5637)
                     /locus_tag="ACG8FM_002392"
     CDS             complement(4822..5637)
                     /locus_tag="ACG8FM_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5095015.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5634..6458)
                     /locus_tag="ACG8FM_002393"
     CDS             complement(5634..6458)
                     /locus_tag="ACG8FM_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFS5095016.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6448..7548)
                     /locus_tag="ACG8FM_002394"
     CDS             complement(6448..7548)
                     /locus_tag="ACG8FM_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5095017.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7726..8511
                     /locus_tag="ACG8FM_002395"
     CDS             7726..8511
                     /locus_tag="ACG8FM_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS5095018.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8544..8927
                     /locus_tag="ACG8FM_002396"
     CDS             8544..8927
                     /locus_tag="ACG8FM_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS5095019.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9003..9134
                     /locus_tag="ACG8FM_002397"
     CDS             9003..9134
                     /locus_tag="ACG8FM_002397"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5095020.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9103..9339
                     /locus_tag="ACG8FM_002398"
     CDS             9103..9339
                     /locus_tag="ACG8FM_002398"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5095021.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9351..9437
                     /locus_tag="ACG8FM_002399"
                     /pseudo
     CDS             9351..9437
                     /locus_tag="ACG8FM_002399"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9417..>9551)
                     /locus_tag="ACG8FM_002400"
     CDS             complement(9417..>9551)
                     /locus_tag="ACG8FM_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS5095022.1"
                     /translation="IELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF
                     "
BASE COUNT         2796 a         2267 c         1850 g         2638 t
ORIGIN      
        1 gttccatgag ttttatttgt gttgatggga caacccatca gctatttacg atccttgaag
       61 atcgcagact ttacaaacta actcaatact tcctgcactt tcctagaaaa gcccgtttaa
      121 aggtcaagta cctagaatgc cagctattgt cagctgctta aaacggtctt tccaaatgcg
      181 gagatcgtaa ctgatcgctt tcatattgtc caacatatca atcgcagctt caatcaattg
      241 cgtatccaga tcatgaatcg ttttcgagcc tcgcactctg aaaatcagaa gaagtaccgt
      301 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
      361 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
      421 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
      481 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
      541 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
      601 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
      661 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
      721 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
      781 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
      841 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
      901 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
      961 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     1021 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     1081 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     1141 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     1201 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     1261 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     1321 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     1381 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     1441 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     1501 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     1561 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     1621 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     1681 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     1741 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     1801 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     1861 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     1921 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     1981 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     2041 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     2101 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     2161 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     2221 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     2281 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     2341 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     2401 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     2461 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     2521 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     2581 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     2641 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     2701 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     2761 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     2821 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     2881 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     2941 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     3001 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     3061 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     3121 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     3181 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     3241 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     3301 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     3361 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     3421 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     3481 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     3541 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     3601 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     3661 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     3721 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     3781 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     3841 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     3901 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     3961 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     4021 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     4081 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     4141 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     4201 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     4261 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     4321 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     4381 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     4441 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     4501 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     4561 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     4621 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     4681 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     4741 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     4801 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     4861 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     4921 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     4981 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     5041 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     5101 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     5161 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     5221 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     5281 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     5341 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     5401 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     5461 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     5521 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     5581 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     5641 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     5701 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     5761 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     5821 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     5881 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     5941 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     6001 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     6061 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     6121 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     6181 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     6241 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     6301 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     6361 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     6421 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     6481 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     6541 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     6601 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     6661 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     6721 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     6781 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     6841 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     6901 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     6961 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     7021 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     7081 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     7141 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     7201 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     7261 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     7321 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     7381 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     7441 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     7501 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     7561 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
     7621 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
     7681 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
     7741 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
     7801 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
     7861 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
     7921 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
     7981 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
     8041 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
     8101 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
     8161 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
     8221 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
     8281 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
     8341 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
     8401 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
     8461 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
     8521 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
     8581 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
     8641 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
     8701 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
     8761 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
     8821 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
     8881 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
     8941 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
     9001 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
     9061 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
     9121 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
     9181 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
     9241 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
     9301 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
     9361 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
     9421 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
     9481 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
     9541 aaaagctcta t
//
LOCUS       DAZUMT010000106         9408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000106 DAZUMT010000000
VERSION     DAZUMT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.108"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      complement(21..87)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(129..776)
                     /locus_tag="ACG8FM_002401"
     CDS             complement(129..776)
                     /locus_tag="ACG8FM_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS5095023.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1055..1558)
                     /locus_tag="ACG8FM_002402"
     CDS             complement(1055..1558)
                     /locus_tag="ACG8FM_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5095024.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1681..2445)
                     /locus_tag="ACG8FM_002403"
     CDS             complement(1681..2445)
                     /locus_tag="ACG8FM_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS5095025.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2552..2827
                     /locus_tag="ACG8FM_002404"
     CDS             2552..2827
                     /locus_tag="ACG8FM_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS5095026.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3504..4454
                     /gene="yidC"
                     /locus_tag="ACG8FM_002405"
     CDS             3504..4454
                     /gene="yidC"
                     /locus_tag="ACG8FM_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS5095027.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4633..5607)
                     /gene="lacD"
                     /locus_tag="ACG8FM_002406"
     CDS             complement(4633..5607)
                     /gene="lacD"
                     /locus_tag="ACG8FM_002406"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5095028.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5604..6062)
                     /locus_tag="ACG8FM_002407"
     CDS             complement(5604..6062)
                     /locus_tag="ACG8FM_002407"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5095029.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6059..7522)
                     /locus_tag="ACG8FM_002408"
     CDS             complement(6059..7522)
                     /locus_tag="ACG8FM_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5095030.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7504..8454)
                     /gene="pfkB"
                     /locus_tag="ACG8FM_002409"
     CDS             complement(7504..8454)
                     /gene="pfkB"
                     /locus_tag="ACG8FM_002409"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5095031.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8597..9346
                     /locus_tag="ACG8FM_002410"
     CDS             8597..9346
                     /locus_tag="ACG8FM_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5095032.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         2794 a         1994 c         1634 g         2986 t
ORIGIN      
        1 aagaaagggg ctatccccag actttagtag gcgtcattag ctatctttcg ttagcattta
       61 aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa
      121 caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc
      181 gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac
      241 gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg acacattcga tgttttctga
      301 cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat cccttttaat
      361 ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt ccagccgatg
      421 tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt
      481 cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctggac gatttgcttc
      541 agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt
      601 aaaaatgtat cgatttctgg cagttcgtgt tctaatatgt ctcgaatctt ttcagctgct
      661 agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac
      721 tgatctactt ctctttttat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt
      781 ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa
      841 gtatgtgtta cctgaagatc ccggactctt ttaaataaga tacgttttgc atcttatcat
      901 cacccccttt aatgttcatt cttctatgta catgattcat tcgataaata aaagtaaacc
      961 gtatcttgaa cagatagtca actgcaaaca gaaaaaaccg agaaaatcag ccagattttc
     1021 tcggaatata gtctattatt attggtttta cctattaaat cgaggattct ttcccaaagt
     1081 aacgttgcca tctcttacgc atacttgcag acatgggctg agccgcttct ttgactgcgc
     1141 agtatttgtc cacgaatgtc acaatatatc cttctttata cttaggcggt gcgatcgttg
     1201 caccccacat gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg gtgatttttt
     1261 ctgcattctt tacagcaatt cttggatgga tatacgcatg tgtcccctcg tcaaatttcg
     1321 tcgtacgcca atcatagtag aacagatcat gcaaaagtcc agcacgcgct gttgctttag
     1381 cattgccacc ccattttttt gcgagcagat aactatagta agaaacactg atggaatgct
     1441 ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact gcttctgttt
     1501 ctaaaagatc ttgtacataa gacatatatt cttcgtcttc acgccatttt tctgtcataa
     1561 ttatcgatct gccacctttt tgaatttcat tttcttctga aattgatcta agtataccac
     1621 cagtgttcgg attattctag tcgctctaga taaaatagct aaagcttaat agttccttaa
     1681 tcataaagaa aaatgataaa gaacgatccc tgctgcgact ccgacattca atgattccgc
     1741 atttcctttc attgggatat atacgttctg atcagtcaaa gacaagattt cctgacttac
     1801 tccctgacct tcgtttccca tgattatagc gaaatcctct gttttttcta ctttgtctaa
     1861 tgcatgtgct tcttcattta attctgtacc gtacaccgta aaccctttgc ctttcattac
     1921 agggattaca tctgccaaat ctttttgtaa cacaggcaaa tgataattgc ttccctgcat
     1981 gcttcgcagc acctttgtac tataaatatc tgccgttcct ttacctaata aaacaccagc
     2041 cataccaaac gcatctgccg tacggatcat cgttcccaca tttccaggat cctgtacatt
     2101 atctagtaaa agccagcctc ctgaaaaatc tggcgtatct tcagatgtta acgtcatcac
     2161 tgctaagatc ccttgcggtg taggtagatc tgacatagcg gtcatgacct catctgaaac
     2221 tagaaaagta gaaatctctt tgtttacagc cagccagtct ccccattctt tcatacctct
     2281 ctgtgtcaca aaaagttctt ttatttctgc attagacgac gcagcttctt cgatcagatg
     2341 gaacccttcc aaaagatatt ctgccttttc ttctctgtat ttctttttat gtagtttttt
     2401 taattctttg attcttggat tttttgctga ttggatctct ttcacattcc tcacctcaac
     2461 tgattatatc atggaatagg tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg
     2521 atttataaaa caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg
     2581 acgtgtccag ggcgtgggat ttcgctatat gaccaagatg gttgctgatc agttaggcgt
     2641 gaccggaacg gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga
     2701 cgacatcatc caaaaattta tcgaagaagt caagaagtct ccgagcccaa gtggacgagt
     2761 gcagtatgtg gatgtccaag aagatccgat gattgaggaa cgaaagaaat ttgatgtagt
     2821 gggataaggt tgtgaaaagg tcgcttagtt ttgagtatta aggaaaattt tgtgaaaatg
     2881 gctttccaca tttttgcata aatttgactt aataccgaga aactgacttt tgaacaccgt
     2941 cgataaggtt gtttttgaag cgctttgcct cgagtattaa ggagaaacat tttcaaaata
     3001 gcttctcata ttttgaaatg taatgactta ataccgagag gcagctgata aaacaccgtc
     3061 aataaggttg tgaaaaagct ggtctgcatc aagcattaag gactggttgt gataaatagc
     3121 tctccatatt tttcaacagg ctggacttaa tgccgtagat gcaagctttt gaacaccgtc
     3181 gataaggttg tttttgaggc gctttgcctc aagtattaaa gacaggtagt aaaaaagctg
     3241 atctataact tgcaatgaat ttgcttaacc catattttcc agacagaaca aaaaagtttt
     3301 tagtttttta aataagaaag aaacacttgg aatcactctt ttctttctaa gtatttcttt
     3361 cttgattttt tgtctttttt caaaattttt cgctacgaaa aagaacgaat atttatatga
     3421 aatttctatg aagttgattg aattccatgc gcttttttag tatagttaag tttgtgtaaa
     3481 aattatagaa gaggaagtat tctatgaata aatggaaaaa ctggttattg ggatccggac
     3541 ttgtcagtat cctgctcttt ttatctggct gtgtgcggat ggattcaaac ggaaaccctg
     3601 acacttcagg aatcgtttat cgagtactcg tccatcctat gggccaagcg attacatatc
     3661 tagttgagaa tttcaattgg tcttacggct gggcagttat tgcaatgact gtgatcgttc
     3721 gtatcatcat cttgccacta ggtatcagtc aatcgaaaaa aacaatgatc caatctgaaa
     3781 aaatgcaagc gttaaaacca caagttgaag cagcacagca aaagctaaaa gctgcaacaa
     3841 cacgtgaaga acaaatggca gcacaagcag aaatgcagca agtatatcgc gaaaatggat
     3901 tgagtatgac cggcggaatc ggctgtctgc ctctattgat ccaaatgccg atcttctctg
     3961 ccttgtattt tacggcacgc tacacagaag gtatccggga atcatctttt tacggcatcg
     4021 atctaggctc accaagtatg gtgcttgtgg ccatcgcagg ggtcgcttat ctgcttcaag
     4081 gatatatttc aacaatcggt attcctgaag aacagaaaaa gacgatgcgt acgatgctga
     4141 tcgtctctcc tgctatgatc gtcttcatgt caatcagtgc gccagccggt gttacacttt
     4201 actgggtagt cggtggtgtg ttcagctgtc tgcaaacatt catcacaaac gttatgatga
     4261 aaccacgttt gaaagcacaa gtggcagaag agttgaaaca aaacccgcca aaacaagtcg
     4321 tcactccaag aaaagacgtg acagaatcag caaagccagc taacaacaaa caagcaacaa
     4381 aacaaatcaa acaaccaaaa aacagtacaa acaacaaagg ccgcaacgca ggaaagcagc
     4441 agaagcggaa gtagatagta aataagagtg taataaaaat tcattaacag ttttagcaat
     4501 taaaaatatc gaccaagatt tttgaaaaag aaatctggtc gatattttta tttaagtgaa
     4561 tgtttaattt gaatcatcga ttgatcgcta tctaattgca gcagcatctt tttatgaaga
     4621 aacaactttt tcttatctat caacgtagac aggtgttgct gtttcttcta atagtgtact
     4681 taattcttta atattttgtt ttccttcttc taacatccaa ctactcgctt tttcttcgcc
     4741 ttcggtaaca aaaggtgcca ctcccttcgc ccatgttgct cgcccacata agacaccatt
     4801 gaatgtcgaa cctgattgtt ttgcaaaacg taatgtatct tgaaatagcg tagcattgac
     4861 tcccgcactt aaaaaaataa acggttgttc agtcactttc gattgttcat agaaatacgc
     4921 ggatgcctct gcttgactat aacaagtctc ttgcccatat ccttccacaa atctcatatt
     4981 cactggcact tctactttca atacatccac ttgataacgt tcttttgaaa attctcgcat
     5041 ggcttcaata actttatgtg gttttacttt tgcataggca agagaagacg gatcgccaat
     5101 agttgcatca taagaaagaa tctctaaaaa gaaaggtatg ccttctgctt cgcattctga
     5161 accaatccgt tctataacta tttgtttttg cacattaatc tctgctggtt catcaacgtc
     5221 atagtacaat aaaaatttac aagcatccgc accagcttct tttaaccttt tcaccgacca
     5281 aatattcaat aaatcaggta atcttcccgg tattttcgta tcatagccgg ttttttcata
     5341 agctagcagt aagccacatt ctttctctcg aagcgctgct gcaggcaggc cgtattctgg
     5401 atcaagtaaa atagctgagg catagggagt caggtattga gaaaccatct gtttaaattg
     5461 gatgatgtct tctgatgtag cttctttata ttggttgatc attttcttca aagcaccacg
     5521 ctggtcgatt gctaatgcgc taataattcc agcttcatcc gtcaatcgct tcaaacgttc
     5581 ctgtttttct atactcatct tcttcataga tcttcaccta tttctttagt taatattgtg
     5641 gctatttctg tttttgattt tgcttttctc aactgttctg taacctcttt tcgcatcaat
     5701 gatcgtgcaa cgagagacaa taatttcaaa tgagtcgttc ctgcttctga atctggaata
     5761 aataacgcaa aaataaaatc aactgactgt ctatctaaac tgttccattc aattcctttg
     5821 ttcagtgtgg cgataatcat actaggttcg ttgattgttt gagacttcgc atgagggata
     5881 gcaaaacctg acattattcc tgtagtccct tctttttctc tttctagaag tttttgatat
     5941 acttcttttt cgtcaagagc gatccctaaa cagaatgttt gctttgctaa aaacttaaat
     6001 gcttcttctt gactatccaa tggctgttgt aaaaaaatgt tctcttcatg aatgattgtc
     6061 atgataagtc tcctgctctt cctttttcga taactgtacg atttgctgct ttattatcca
     6121 tcttcttgtc tttatcagtt actcttttct tttggattaa tcctagtatc actccaccaa
     6181 cgatcgaacc aataagaatc gctgtaaccc attggatttt tcccgtcact acaggtaaca
     6241 ctaaaaaacc accatgtggt gctggaactt ggacgccaaa caaataagtt aagacagcag
     6301 caattgatga gcctaacatc atcgtgggta acactcggat cgggtctttc gcagcaaacg
     6361 gaatagctcc ttccgtaata tgagttgatc ctaaaataaa attaaccaaa cctgcattac
     6421 gatcattggt atcaaaatat ttaccaaaca ataaagtagc aaaaccagta atcaacggtg
     6481 gtgcaataca agctgctgac acaccagcca taaaagtcgt gtttccttgt gccaaaagag
     6541 ctgttcctgt tacatatgcc gccttattaa ccggtcctcc catatcaaac gcacacatac
     6601 agccaacaat cacgccaaga acaagtggat tcgaatgttc aaatccagct aaaaagctca
     6661 tcattccttc atttatagcc ttcattggac ctgccagcaa agacatcacg atgccaataa
     6721 atgctacacc aataactgga tataagaaga ttgccttcaa gccatctagt tgtttaggta
     6781 aaattgtcag tagcttttgc aaaaataaga taaaataacc tgctaagaac ccagctaaaa
     6841 tacctcctaa aaatccagta ccgccattca tggctacgat tccaccaaca aatccaacga
     6901 ctaaccctgg acgtttcgcg atcccttctg caatataagc tgctaaaact ggaaccatca
     6961 ttcccattgc tgcatcgcca actgctttta ccattgccgc tgtagcattg tattgtgcat
     7021 gatctggatc aaaagaatag attccccaaa cggcaaatga aatagcaatc aatactccgc
     7081 cagctaccac gaaaggcaac atatgagaga caccgttcat taaattttta tagatgctac
     7141 gaccaaaatt agcaccactc atcgcttctg gttctaaagt atcagcagct tcattatctg
     7201 ctaagacagt accttttcct gccaatgctt cgtcaatcag ccgttgtgca tccttgattc
     7261 ctgtactgac agaaacatca atgactcgtt ttcccgcaaa tctttctgaa tggacatctt
     7321 tatctgcggc aataatgaca gcatctgcct gtttgatttc ttcagccgtt aattcatttt
     7381 cgatcccaat ttgtccatgg gtctccactt tgatcttgat gccttttttc ctagctgctt
     7441 gttctaatgc ctcttgcgcc atgtaagtat gcgcaattcc tgtcggacaa ccggttgctg
     7501 caattagttg atattttccc atgtttcaaa cctctcttcc ttgattttga tttgtttact
     7561 taattcctcc acatccaaaa aatcagtcag tcctttacga aaagcggttg agctgcctgc
     7621 tgctatgctt ttacgtaacg tttcatcaag cggtcgtcga ttcatatatc ctgctaaaaa
     7681 agcgcctaac atggtatcac ctgcacatgc tgtattgaca acttttcctt ttggcgagtt
     7741 tcctcgaaac accctgtctt tcgtaaaaag aatcgctcct ttctcaccga gggataacaa
     7801 aacattttct gcacctaact ctactagtcg ttgtccataa acaaagtaat cttcatctgt
     7861 aaccattttt cgtccgaacc aagaagaaag ttcttcctcg ttcggtttca gcaaaaacgg
     7921 tcgatagggt aaacaatcca tggcttcttg atcactacta tcaataatca aatgaatccc
     7981 cttttcctga caaatccgac tgatttctat taaaaccttt ggagacagcc ctcttggtaa
     8041 actcccagaa acacaaagat aatcttctgc ttgtaatgct cttatttgat ttaaaaaatt
     8101 atggacagct gtctgcggta ttttaggtcc ttgattcact aatttgtatt cttctcccgt
     8161 ttgattgact tgtgtaaata cattgatacg cgtcatacca ggcacttcaa taaactctgt
     8221 ttggatttgt ttctgctgta aatagtcttc tatatacttt cctgtaaacc cagcagaaaa
     8281 acctaatgcc gtacttggga aatctagcat ttttaatact agtgacacat tcaccccttt
     8341 tccgtttgcc tgaatgtcat cctcttttgt gcggtttacc ataaaaggat gcatctcctc
     8401 tgtttcaata aatagatcaa ttgccaaatt catcgtgcat gtgtagatag acataaccaa
     8461 cgacctcctt gactctatta tgaaatacgc gaaagagaaa atgttttctt aatcgtccca
     8521 acacaaagaa aacctttaca tttataaact cgtcgtgcta ttttttaact aacttttggt
     8581 gaaagaagga aagaatatgg atcacaaatt aagtgaaaca gaacagtacc tctggaactt
     8641 tatcgaacac cacatcctag agatccctaa ctactccatc gtgaaactaa gtgaacaagc
     8701 aaatgtctca acagccacca ttgtgcggac aatgaaaaag aaaggctatg aaggatttac
     8761 ttcgtttaag caccatctaa aagaaggaga aaacaagaac atcaattttt ctttcttaga
     8821 taaagtagac aaaggtatcc gtcgagccat tctaaaaaat gaacaagaag tggtacgtac
     8881 gatcaatatg ttggaaatcg gaaatattga ggatgcaata caaagaatca aatttgctga
     8941 ccgggttttt gtctttgcac gaggattttc agaaatgatt ggtcaagaga tgctcgtaaa
     9001 acttcaatta acagggaaaa attgtcaaat gcatactgat ccagaaatca tcaaaagcat
     9061 cagtcggaaa ctaactaaga aagacagcgt tatttttgtt tctttgaacg gcgagacaaa
     9121 agaactcgtc accgccgcaa agaattgcta tgatgcagag attggttcga ttctagttac
     9181 agccaatcat tcctctacac tgaaagacta ttgcgagatc aatctcgtag gtttcaaatc
     9241 agaaggttct tatttccctg attatgaagt acggtcacgt ttgcctctcc agattatagc
     9301 gagaatcctg cttgacgcct atgtattgcg tatgggggag aattgaaaag cagtttatta
     9361 tggttggtta cagtttaaaa catcacattt tttattcttt atattgta
//
LOCUS       DAZUMT010000107         9277 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000107 DAZUMT010000000
VERSION     DAZUMT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.109"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..676)
                     /locus_tag="ACG8FM_002411"
     CDS             complement(8..676)
                     /locus_tag="ACG8FM_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5095033.1"
                     /translation="MKKLNLFTEMCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            810..1097
                     /locus_tag="ACG8FM_002412"
     CDS             810..1097
                     /locus_tag="ACG8FM_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095034.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1513..3051
                     /locus_tag="ACG8FM_002413"
     CDS             1513..3051
                     /locus_tag="ACG8FM_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS5095035.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3048..4013
                     /locus_tag="ACG8FM_002414"
     CDS             3048..4013
                     /locus_tag="ACG8FM_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095036.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4000..5322
                     /locus_tag="ACG8FM_002415"
     CDS             4000..5322
                     /locus_tag="ACG8FM_002415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5095037.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5385..5489
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5563..6306
                     /locus_tag="ACG8FM_002416"
     CDS             5563..6306
                     /locus_tag="ACG8FM_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5095038.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6321..7313
                     /locus_tag="ACG8FM_002417"
     CDS             6321..7313
                     /locus_tag="ACG8FM_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5095039.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7325..8062
                     /locus_tag="ACG8FM_002418"
     CDS             7325..8062
                     /locus_tag="ACG8FM_002418"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095040.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8052..8774
                     /locus_tag="ACG8FM_002419"
     CDS             8052..8774
                     /locus_tag="ACG8FM_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5095041.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8858..>9277)
                     /locus_tag="ACG8FM_002420"
     CDS             complement(8858..>9277)
                     /locus_tag="ACG8FM_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5095042.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3033 a         1603 c         1962 g         2679 t
ORIGIN      
        1 ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt tctgtagtct
       61 gttttgcttc caattggggc aaaacagatc caatttccag atacttcact ttattcaatg
      121 tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga tccaaataaa
      181 gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca atcttaatat
      241 gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt ccgccgaatc
      301 gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta ttgatcagta
      361 gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag aaaactagcg
      421 caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac aatgagataa
      481 gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca aaaagattat
      541 gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga atattgattc
      601 ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacatt tctgtaaata
      661 aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa cttctttatt
      721 atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt tggctgtttc
      781 tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg acaattctta
      841 caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa caaaaactgg
      901 tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta tttgaacacg
      961 gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac cgtgagaaag
     1021 ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa gagatagaaa
     1081 ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc aaaacaacca
     1141 ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt gaacaggcca
     1201 ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt tattaaaata
     1261 aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt atagattttt
     1321 atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt tgcttggatt
     1381 ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg ctgtaaggtt
     1441 aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa gaataacaga
     1501 aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg cgagttgctg
     1561 gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc agcactgatt
     1621 gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt cttattgcag
     1681 atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact tatcccattt
     1741 gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt gcttccgatt
     1801 ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga agcagcggta
     1861 gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat cgcattacct
     1921 gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc tactttggca
     1981 gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga ccgaaataca
     2041 cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat attcagcgga
     2101 ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt agcagcagtg
     2161 gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa tgaaaccatc
     2221 acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta taaagaattg
     2281 attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa gacgagtttt
     2341 ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac aggtactgtc
     2401 ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac aaaagaaaaa
     2461 acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt attaaaacca
     2521 atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa cgaacatcaa
     2581 cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg attcacactg
     2641 gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg cttgaatttt
     2701 tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa tggagatgtc
     2761 aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct agtgactcta
     2821 gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa aaaagatttt
     2881 gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa aataacagag
     2941 gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc gacagaagta
     3001 gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg actaaattaa
     3061 tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat gacctaaatc
     3121 tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt ggaaaaacaa
     3181 ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc tacttcaaag
     3241 gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga tatgtgctgc
     3301 agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc atccctgaaa
     3361 tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt caaaaagttg
     3421 gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga ggagaacagc
     3481 aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg atggacgagc
     3541 catttagcgc attagatccg atttcaagaa actcattaca agaattggtt ttgtccttac
     3601 acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa gcaatcaagc
     3661 taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac acaccagagc
     3721 agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa ccgaaacaga
     3781 cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat gaaataccag
     3841 aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat tcgcttcttt
     3901 cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg ctgacaagta
     3961 aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata tgatgcaatc
     4021 atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga agcaggtaaa
     4081 acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg tggatgtgat
     4141 cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca attgagtgga
     4201 aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt caaacgaaca
     4261 tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga aatcgatcat
     4321 ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga agaagtcttc
     4381 catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc agtggaggga
     4441 aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc taaagaaatc
     4501 atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa tgctgcaggg
     4561 agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga ggcgtctttg
     4621 gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt tggtgttgaa
     4681 acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga gacggaatta
     4741 gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc tttagcactt
     4801 gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta tctgcaaacg
     4861 agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc aaaattgaca
     4921 ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc aacaagcgaa
     4981 cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact tgctgaagta
     5041 ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga tcttaccagt
     5101 tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac ctttgaccag
     5161 caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt gatcgatgat
     5221 ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta tatcatgggg
     5281 tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg ataactaaag
     5341 cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg tttctgggga
     5401 tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg tcagttaata
     5461 ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt gcccatttca
     5521 agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa aaatctttat
     5581 ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat agtttggaaa
     5641 atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc ggcagatgca
     5701 caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg tttggtaata
     5761 gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt tttagaaatg
     5821 tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt agggccatta
     5881 cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt cttgataagc
     5941 tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa agagcaaatc
     6001 atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata taaaatacca
     6061 gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg tgtcagcgga
     6121 gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat gatacgcgta
     6181 aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat tctattgagt
     6241 ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat gctttatatt
     6301 ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta ctgttgcttt
     6361 tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag gatttgaaaa
     6421 aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc tacctagcaa
     6481 aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa ccaggagata
     6541 attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta agttatcaag
     6601 aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca attgctacag
     6661 tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc cattcaccaa
     6721 aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa gctgtcttaa
     6781 aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta ggaagcaatg
     6841 gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt gaaggttggg
     6901 atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg aaagaattag
     6961 atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg atcaaaagtg
     7021 atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag gaagctatta
     7081 aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat gatcggacgt
     7141 ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct gagaattggg
     7201 gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa aacggattga
     7261 tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa taggagtgat
     7321 agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga aaatattaga
     7381 agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc caagcggagc
     7441 cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt ctggaacgat
     7501 caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata tgccgcaaga
     7561 agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc aaaaaatcaa
     7621 tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg ggttaaacgg
     7681 ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa cggccttgtt
     7741 acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg gagcgttaga
     7801 tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa aagcaaagcg
     7861 gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag acaggattct
     7921 tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg aaaaacgacg
     7981 gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc agttattggc
     8041 aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag agtttagcac
     8101 tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca gaagaatgga
     8161 aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt atccatcctt
     8221 ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa gcaaggatca
     8281 tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca caaaaaaaag
     8341 tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta ctgcacacaa
     8401 aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac tatttgatcc
     8461 attggtattc ttctttggac tatcaacaag actatattga tttaggctgt tatttcagca
     8521 ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca gtcccaatcg
     8581 accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta ggggggattt
     8641 tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc gctgagatca
     8701 aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa cgtttcatta
     8761 gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt ttggctttct
     8821 ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg tttgcttccc
     8881 agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt tctgctactc
     8941 tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat tggtaacgtc
     9001 cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt gcgatccatt
     9061 cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca ggagcaggtt
     9121 gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt acaacaggtt
     9181 gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct gcttgtgcag
     9241 agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DAZUMT010000108         9192 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000108 DAZUMT010000000
VERSION     DAZUMT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.110"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..786
                     /locus_tag="ACG8FM_002421"
                     /pseudo
     CDS             278..786
                     /locus_tag="ACG8FM_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            879..2041
                     /locus_tag="ACG8FM_002422"
     CDS             join(879..1155,1155..2041)
                     /locus_tag="ACG8FM_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5095043.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2321..3301
                     /locus_tag="ACG8FM_002423"
     CDS             2321..3301
                     /locus_tag="ACG8FM_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5095044.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3423..4841
                     /locus_tag="ACG8FM_002424"
     CDS             3423..4841
                     /locus_tag="ACG8FM_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5095045.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4912..6381
                     /locus_tag="ACG8FM_002425"
     CDS             4912..6381
                     /locus_tag="ACG8FM_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5095046.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6391..6858
                     /locus_tag="ACG8FM_002426"
     CDS             6391..6858
                     /locus_tag="ACG8FM_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5095047.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6894..7766
                     /gene="scrK"
                     /locus_tag="ACG8FM_002427"
     CDS             6894..7766
                     /gene="scrK"
                     /locus_tag="ACG8FM_002427"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5095048.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7847..>9192
                     /locus_tag="ACG8FM_002428"
     CDS             7847..>9192
                     /locus_tag="ACG8FM_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5095049.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRK"
BASE COUNT         3169 a         1354 c         1840 g         2829 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaggaaata agaaaactaa acttgtatcg
      601 ttaacaaaat atggtcgaaa ggtgtacgaa tcacataatt atttacatga agagctttat
      661 agctcaataa atgaagcgtt agaatcattc tcaccagacc aaatttcaac cattaaacag
      721 attctagaaa aagtatctta tacaatcaat gaatatcaag taagtctgac aagaaaggaa
      781 gattgacatg aaaaaaatga tttttgaatc ggctacacta gactagacag aaaaaataag
      841 gtgtgtaaaa taaatgaaaa cacactggag gaatttttat gtcaagacgt caacgtagaa
      901 cctattcaaa agaattcaaa caacaagtcg tcgatcttta tctcgcagga aagccccgag
      961 ctgaaatcat tcgagattat gagctgacta cctcggcctt tgataagtgg gtcaagcaag
     1021 cgcaaactac tggctcgttt caggaaaaag ataacttgat ccctgaacaa gtagaactaa
     1081 ttgcattacg aaagaaaaat aaacagcttg aaatggagaa tgacatttta aagcaagcgg
     1141 cgctgatatt gggacgaaaa gacaagtaat tgataccaat aagcacaaat actcaatatc
     1201 agcgatgtgt aaagtgttaa acatctcttg ccaaacctat tattatcaag ccaaaccagt
     1261 tgaagatgaa tcagaactgg aagaactcgt tcaagaagaa tttattcgca atcgaaaagc
     1321 ttatggtacc aggaagctga agcagtgctt ggccaatcta ggtcttcaag tgagtcaacg
     1381 cagaatcggc cgtatgatga aacagcgtgg cttgcgttca acatacacat tggctcattt
     1441 caaattccat tcttctactt gtaacgaagc caagaccgcc aatattctag acggaacctt
     1501 cctacaggaa aagtcattag aagcgattgt aaccgacctt acctatgttc gtgttgggaa
     1561 gaaatggcag tatatctgcc tgattcttga tttgtttaat cgagaaatca tcggctattc
     1621 atgcggggag aagaaagatg cgacgttagt caaggaagct tttgcaaaga ttccgtactc
     1681 gttgaataat gtacaacttt ttcacacgga tcgtggaaaa gaattcgata accagacgat
     1741 tgacgaaatt ttatgggcct ttaatatcac gcgttcctta agcaaaaaag gctgccccta
     1801 tgataacgcc gttgcagaat ccacctataa atctgtcaaa gtagaattcg ttcatcaata
     1861 ccgctttgag acattggcta atttgcgatt agagctattt gattacgtcc attggtggaa
     1921 taccatccgg ctgcacggca cgttaggcta tgagacaccg atcagttttc gacagcagag
     1981 attggcgcag cgaatccttg ataatgagct cggatgtgat accggtggcg aggcacctta
     2041 attatttgaa tgagcttctg ccttagagaa tcagattcga ggaggctcgt tgtcaagggc
     2101 agtcggaaca ccagcagcaa gcttacgcaa taccttataa tttttgtcaa aaaaagtgtt
     2161 gccattccac atatatcttt aaacagctat tgccagataa aaataaatga tattatatat
     2221 ataaatactt atccgaaaag gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc
     2281 gagtaggtag attttctatc tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag
     2341 agaagttgcg aaagaggcaa atgtttctcc tacgactgtg tcgagggtat tgaatgatcg
     2401 tggatatttg agtgaggaaa caaaaagaaa agtttttgat gccatgaaaa aattagaata
     2461 ctatcctaat gaactggcga gagcactttt taaaaacgaa acatataccg taggtttgat
     2521 ttttcctaca attactaatc catttcacgc tgagttaatt caagatatcg agttcgagct
     2581 ttccagcagg ggatataaag tgttgctgtg caatagctta aacaatccaa caaaagaaaa
     2641 agagtacttg acgatgttga gaaagaatca ggttgatgga attatagtag gaacgcacaa
     2701 taaagatatt gcggattatg atattccagg gctacctatt gtagcgattg atagaagtct
     2761 tggtgaaaat acagttacag tatcttgtga taattacagg ggtggggaat tagctactca
     2821 actacttgta aatcatggct gtcagaatat actgtgtatt cggggagata gtaagataaa
     2881 gctacccgcg aatagtagga ctttagcata taaagatgtt ttgaataaat tcatgctgga
     2941 accgttagtc ctagaagtac catttgtaaa agaaatcaat gaaaaaaatg aaataattaa
     3001 gacatatctg gaaactcatc caaatattga cggggttttt gcaggagatg atttattggc
     3061 ttctttggcg attaacaact taggaagttc gggcaaaaga gttccaaaag aggttaaagt
     3121 gattggattt gatggagctc aacaaacatt gatttataat ccagaattat caactattca
     3181 acagcctata gatcaaatat ctaaagtagc agtaaaaaaa ttattgaata tgattaaggg
     3241 cgaaaaggag acggatgaac tttcacttcc agtaaagctc ataaaaaaaa atacagcata
     3301 agttaaatcg gcgaaaaatt ccttcgtcga tttaaaaata tttttatgaa acgggttgac
     3361 atatgaaacg ggttgacata tattagttgt gaagataaaa accttttcac tggaggaaga
     3421 aaatggagaa attattaatt aaaagcaata atcatcaact ttactaccaa cctaaggatg
     3481 tttgggtcgg agatgttatg ccttacggga aagaaggaaa attctttatt tatcatcaac
     3541 gtgatactag aaatccagtt ccttttggag aaccgtttgg ctgggcttta gcaactacga
     3601 aagattttgt tcattttgaa gactttgggg aatctctttt aagaggggat gacaatgaga
     3661 gagaccagtt tatctacgct ggttctgtat ttgaggcaaa tggaaaggtt cacgcatttt
     3721 atacagggta taatcgtgaa ttcttagcgc agggaaagac atctcaagtg ttgcttcatg
     3781 ctgagagtga tgattttgtt cattgggaaa aatcagaaac agcattggag ttagaaccac
     3841 aagaagggta tgatattcga aactggagag atccatttgt cgtttgggat gatgaaaaag
     3901 aagaatattt gctaatttta ggggcgcgaa agggtcagga taaacatcta caaaccggac
     3961 gtttagtgga atttacatca aaagatttaa ctaattggga attcaaaggt gatttttggg
     4021 ctccgggatt gtataccatg tttgaaatgc cagaactttt caagattggt gactggtggt
     4081 atcttgtcta ttcagaatac tcagatggaa ataaaatact ctatcgaatg agtcaaagtt
     4141 tggaaggtcc ttggattaag cctaaagatg aagcttttga tggacgggct tattatgcag
     4201 gtagaacagc ttatgatgga aaacgtcgag tcctatttgg atgggtacca acaaaagatc
     4261 aagaaaatga taaaaacaat tatttatggg gaggtacttt ggttcctcat gaagtctatc
     4321 aaaaagagga tttttcactt ggggtaaaac ccatagacga agtgtggaat gcattcataa
     4381 atttacatga tattgccgat gtagagatag aaacagcaga cacaagagaa gaagtaacgc
     4441 ttgtcaaaaa tagtggaact ttgtttaatt tagaaacaac aatttcattt gatgagaatc
     4501 ttagtgactt ttcactgaga ttatataaaa accttgagac agatgaatct tacgaatatc
     4561 gcttcaattt agatgagggg cgtcttacat ttgataaaaa cccttgttac ccttggtttc
     4621 aatatatgga taaaggactt attagaccta tatctttaaa gcctaaccaa tcttataaac
     4681 taaaagtgat tgttgatgac aatataatga cactttatat tgatggaatc gctcttaacg
     4741 ctcgtgtaca tggttgtttt ggtcaagcaa ttactattac agcaacaaat ggatcaatga
     4801 ctgcaataaa taccaagttt tcaaatgaaa taaagaaata ataaacgtca atcatgtggc
     4861 aggtctgaaa atcaaacatg acctgtcaca tttaaaaggg aggataaatg aatggactac
     4921 aaaaaaattg ccgaagatgt attggaaaac gttggaggaa aagagaatat ctcaagcgct
     4981 acacactgca ttactcgttt acgtttaatt cttaaagaca aaagcaaagc gaatatggaa
     5041 atgcttgagg aaatagaagg tgcaaaaggt gttcttttca atagtggaca gttacaagta
     5101 attttcggaa caggtataat tgaaaaggtt tacaaagagt tcgttgaatt gacaggagct
     5161 aaggaagtat ccgtttctga aatgaaagac gaaggagtca ataattcagg aaatagactt
     5221 caacgagctt ttaaagtttt ctcggatatc tttattccga tcattccagc ttttattggg
     5281 gcagcaatgg ttttaggaat tcgttcgttg attaccactc ctggaatgtt tggaatggat
     5341 ggctcattcg ctgatcacaa tgtattccta aatgatttat ccaaatttct gggtattatt
     5401 gcaaccacat ttgattattt accagtttta gttatgtatt ctgcaacaaa acgttttggc
     5461 ggaaacccta ttttaggatt acttgtagga tttgttatga ttcatccagg actagccaat
     5521 cggaatgatt tcgtcatggg aactataaag cctgattatt ggcatttatt tggattaagc
     5581 attccagcag ttgcgtttca agggggtgtt tttccagcaa ttttgacttc ttggttttta
     5641 gcaaaagttg agaaaattac aaataaatat tctcctcaag tacttgctta cattttagtc
     5701 ccaactgtga caattgttct tgccaacttt gcactatttt tagtgtttgg tccattgggg
     5761 aattttatcg gtaatgtttt aggtggaatc attgatttcc tatatatgaa gatgggtgcc
     5821 tttggtgctt ttgtttttgc aggcgcgtta caaccattag ttgtaacagg aacacaacat
     5881 gctattcaag gtattgaggc aaacttagtc gcaacaactg gattcaatta tatccaacct
     5941 atttggtcag tttcaattat tgcccaaggt ggcggttgta ttggtatgta tttactcttt
     6001 aagaaaaaat ctaaagatag agatattgcg atttcaagct ttattccaac actggtaggt
     6061 attactgaac cggctatctt tgctgttaat ttgaaatatt caatcgttcc ttttgtttgc
     6121 tcgtttattg gtgctggttt tggtggagca tttatgaaaa tttttgatgt aaaagccatt
     6181 ggacaaggat tgactgtatt gccaggtctg actattgcta atccagttct tccttatata
     6241 tttggtaatc tcatagcatt cattttacca atcattttta ttctcgctta tgataaatat
     6301 aaagtgttta tccctgaagc taaacaccgt aaatttgcag aaaaaactga ctcaaaaaaa
     6361 ttgccagttc aagtaaagtg agatgagagc atgttaaatt tttttagaaa aaatgaaata
     6421 agcgttgtag cacctgcaac cggtgttttt gaaaaactgg agaaattaag tgatccagtc
     6481 ttttcaaaag gaatgatggg acaaggattt gcaattgatg caaaaaacga aacgattgta
     6541 tctccaatcg atggttttgt ttcaagtatt tttcctacaa aacatgcctt aggaataaaa
     6601 acaaaatcag gaattgagat acttgttcat gtaggaatag atacagttga gctaaatggt
     6661 gagggatttg atattaaagt tcaagaagga caaaaagtta aggctgggga tattcttatg
     6721 accattaatt ttgtagtttt tgaaaagaat cacaagacta aagatgtcat tgttgttttt
     6781 ccagactacc aagacgaaat tttcattccg aaacttgaag aagtaaaaca aggacaagaa
     6841 ttgtttgttc ttggctaatg ataagtaatt aataatagaa tcgaggaaaa tgtatggttt
     6901 ttggggctat tgaagctggt ggaacaaaat ttgtttgtgc cattggagat aaagatttga
     6961 acatatttga aaaagtcagc tttcccacga ctacacccaa agaaacaatg aatttagttt
     7021 tagatttttt taatcatcat aaagatgatt tagaaggtat cggagtggga tcgttcggtc
     7081 ctattgatat tcattgtgaa tctaaaacgt atggcttcat taaatcaacg ccaaaacttg
     7141 cttggcagga ttttgatttt attggaagtt taaaaaagca acttaatatt ccaactgctt
     7201 ggacgactga tgtaaatgcc tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc
     7261 tttcaagtgt tgtttactat accgtaggaa cgggcatagg aggtggcgct atccaaaacg
     7321 gaaacttcat tgaaggtttt agtcatccag aaatgggaca tatgcttgta aaacgacaca
     7381 ataaagacaa cttttctggg acatgtccat tccacggaga ctgtttagag ggcttagctg
     7441 ctggtccggc aatcgaaaga agatcaggga aaaagggaca agaagtgact gaaaatgacg
     7501 ttacttggga aatagaagct tattacattg ctcagtgtgc atataataca acattgatgt
     7561 tatcacctga tgtcattata tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga
     7621 aagtacaaga taagtttatt gagttgatca acggatatgt cgctattccc gatgttgaca
     7681 attatattat gacaccaaaa ttgttagata attctggtat tgtaggttgt ttagctttag
     7741 caagagacgt taaagaaaaa tcttaatttg gaaaaaagat cgtctcgact atttgtttgg
     7801 ttgatgcgat ctttttttca attagtaatt gaagggacgc ctaaaagtga aaaaaaaaat
     7861 gaaagatgaa gtattagagt atatcaaaaa taatttaaag tattacgatt tttctgctca
     7921 agatattgca atgaaatttt gtattaaaag aaatgtcgct tcccactatt tgaatcagtt
     7981 attttcagat ggaaaattac taaagaatga ttctgttaga ccagtaatgt tcaagtataa
     8041 tcaacaaaag ccaaaagact gcttttctaa gtttattgga gcagacatat ctttgaaatc
     8101 cactatagat aaatgcaaag ctactgtaat gtatccccct aatggcctcc cattaataat
     8161 aaaaggaaac agtggcgttg gaaaaagttt tttagcaagt ttgatctatc agtatgctct
     8221 tgatagaaaa gtaattcata atgacgcaaa atttgttgtt gtgaattgtg cagattatgc
     8281 aaataatcca gagctattgt cagcagtttt atttggatat aaaaaaggag cctttactgg
     8341 ggctgaaaaa gatactgcag ggatgctatc ttctgctaat ggaggatacc tttttttaga
     8401 tgaggttcac aatctaagtg cagaaaacca agagaaattg tttcttctta tggattctca
     8461 aaagtatcgt aaacttggtg aaagtgtgca atgggagtat gcaaatgttc gccttatctt
     8521 agccacaaca gaagataaaa atacctcttt gttagcaact tttagaagac gcatcccagc
     8581 tgaaatcact ctgccagatt acaataatag aagtacatct gaaaaaattc aattattatt
     8641 tgaatttctt aaagatgaag caattacgat tgattcaaaa atattttgtt cgattgaact
     8701 ttttactgat cttttatcga aaacatttga aggaaatgtt ggagaattga ggaatgaaat
     8761 caagcttctt tgtgcagaag gttatttagg aaataagaga aagtcttcaa tttatttagg
     8821 ggacaaacta gacagcggtt tttggataga tccagaaatt agtatagatt taaaaaaaat
     8881 gtttcttaaa tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat
     8941 tgaagaaagc atgagtcaat taagagatag aatacttaaa gagtgtgcag gttcttctga
     9001 gtataattat ttagaaagtg acgaatttat agcattaaga aactatgttg ttaataaaat
     9061 atcaccaatt attgattcga caggtctatg tttattagat gaattcttgg cggatatcag
     9121 tctatttata atgtttattg atgtaattga agaaacatca agaaattttt tgctgtctaa
     9181 gttcagaaaa tt
//
LOCUS       DAZUMT010000109         9080 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000109 DAZUMT010000000
VERSION     DAZUMT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.111"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..416
                     /locus_tag="ACG8FM_002429"
     CDS             60..416
                     /locus_tag="ACG8FM_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5095050.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            406..672
                     /locus_tag="ACG8FM_002430"
     CDS             406..672
                     /locus_tag="ACG8FM_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5095051.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            831..1832
                     /locus_tag="ACG8FM_002431"
     CDS             831..1832
                     /locus_tag="ACG8FM_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS5095052.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2202..2816)
                     /locus_tag="ACG8FM_002432"
                     /pseudo
     CDS             complement(2202..2816)
                     /locus_tag="ACG8FM_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3056..3301
                     /locus_tag="ACG8FM_002433"
     CDS             3056..3301
                     /locus_tag="ACG8FM_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095053.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3399..3620
                     /locus_tag="ACG8FM_002434"
     CDS             3399..3620
                     /locus_tag="ACG8FM_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095054.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3942..4622
                     /locus_tag="ACG8FM_002435"
                     /pseudo
     CDS             3942..4622
                     /locus_tag="ACG8FM_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4762..5301
                     /locus_tag="ACG8FM_002436"
     CDS             4762..5301
                     /locus_tag="ACG8FM_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095055.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            6321..7490
                     /locus_tag="ACG8FM_002437"
     CDS             6321..7490
                     /locus_tag="ACG8FM_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5095056.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            7963..8517
                     /locus_tag="ACG8FM_002438"
     CDS             7963..8517
                     /locus_tag="ACG8FM_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5095057.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            8573..>9080
                     /locus_tag="ACG8FM_002439"
     CDS             8573..>9080
                     /locus_tag="ACG8FM_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095058.1"
                     /translation="MKITEFSEVFKTFSNIYNRIPEIEQNVVVDNDYYIENDFNKPRK
                     YDNLYNFIHNRTTINNNSNTRVLINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAI
                     NNGNLPSNKKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIF
                     ASIHDYIGL"
BASE COUNT         3391 a         1201 c         1579 g         2909 t
ORIGIN      
        1 taattcgaat ttttatgtta tatactataa tataaaatca atatatgata taattgggta
       61 tggaaaaaat atcttttgaa acatttaaaa gacctaatgg tcatgatgaa tttttagaat
      121 ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata gaagaaacag
      181 aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta gacactaatt
      241 tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agctatctat ttccatgtgg
      301 tcaatggaag gtatgtcatt acccatggat ttactaaaaa aacggagaaa acaccaataa
      361 gtgaacttaa acatgcaaaa gaacttagag aggagtggtt cacaaatgat gattaaaaaa
      421 ttgattgaag aaaagcaaaa agacaaagat tttgcagaag catatgaccg tgaaggaaga
      481 aaactcgaaa ctgcggttgc tctttatcat gcacgggagg aagcaggcct aactcaagcg
      541 gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga gagaggggat
      601 aacgtatctt tcgataaact tgatgatatc gctagggcat taggcaaaaa gctggtagta
      661 gaatttgctt aaaagcgagt tgaaaatata cctgtaatag gctcctaact aatcaattag
      721 tttagtgggc ctttttgtac taaatagata aaagttgtcc tatagtgtaa aagtcaataa
      781 attttataca aaaattactt taaagagaca taaagagaaa ggtacaataa atgaaaacca
      841 taaatctaat aaaagtattt atagcatcac caggtgatat agcagcaaag aggaatgaag
      901 tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa gtaattttga
      961 tgccaattcg atgggaaacg aatgcgattc ctacttatat aaataatagc tctgggcaaa
     1021 gtgttattaa taaacaaatt gtagaaaata gtgatatatt attagcgatt tttggccata
     1081 agttgggttc tccagttgat aattatagat cgggaactat agcagaaatt gattactttt
     1141 ataaaaatag caaaggcgag gtaggggttt ttttcttaga tgaaccagtt ccacaagttt
     1201 tggctgaaga gtatatagaa gttaagaagt acagggactc tttagaagat aagggttttt
     1261 atggaacata tagcaaagac aatatcagaa ggtttctcac aaaaaaagtg aattatttaa
     1321 ttgagaaagc acaaaataat ttagaatcaa aacgctttga tttagattct atttcttaca
     1381 agacaccttc agtaattaac aaaaaagtta ccggaactga taaagatttt gatgacaact
     1441 tagatatttt tgatccaata gaatttgaca atgatgaaag attgctgcta atatatgcgt
     1501 ttatggatga aaattctatc ttttcaactg ataattcaac ttatagaaaa gtagttcaat
     1561 gggaaagtga taacaattta aggagttact tgagtgatag agtatttgat gtactagaaa
     1621 aattaagtaa atcgggaatt ttaaaggaag aatacgattc ttcttttgat gagtatccat
     1681 cacgctatat tttgatttca gaggactata agaaatttaa aaggtatttt ttaaaaaatg
     1741 aaaaaaagat atctgttatt atggagaaat acaaagctcc aatacaggct gatccatttt
     1801 ccaatttgga tgaatcagat ttaccgtttt aaattttaaa tagataattt cattttttag
     1861 tagcagaggt ataaactttt ttaatgtttg aattgaaaag aaatgctaat aggtcgactt
     1921 tatgtttctc ttgtgcaatc gtaaatgcgg gggaccataa aattgatcta ttatcgagtg
     1981 tgtataaaat aaaattcaaa tcttattagt ttacataaga tacattatac taagttgtca
     2041 atgttaaatg cctttaaata aggacgattt atgcatcaat tatatttatg ttaactaaat
     2101 tctttttaac ataaatgtaa tcaacttagc tagatgaatc taaaatgatt gtctgtattg
     2161 atgacaaagc taattaataa ttacgtgatt caaaaaagaa atcatacaat tagttggtcg
     2221 atttcttttt ttattcggta aattgtttgc ctagtcacat tgtagtctgt tgcaatcttt
     2281 gagatagcaa ccccattctc aagatcctga acgatactcc tatagacaag gcgacgttga
     2341 ggatctttgg tgtctgcact gtataactta ggacgacctt tatagacgcc gtttgcttta
     2401 gcgattttaa ttccttgtgc ctggcgtcgt ttcgattcag tacgttcttg ttcagcaatc
     2461 atggctgaga tttaaatgat taagtctttg atgaatttat ctaggagagg atttccaatt
     2521 gcttcagcca tgatcggtaa actggtgata ataagtcgca cattcttctt ctttagataa
     2581 ttcactgaat cgataatctc gtcataattg cgtccaagtc gatcaatggc ttctacaata
     2641 aaaatatctt gctctctgac aaaatctaaa gcttcctgga aaatcggccg atgactaatc
     2701 gtcgctccag attgtttttc gataaaaatt ttttcagcgc caaacttttt catttcttca
     2761 atttgacgtt gttcattttg atcgatggat gaaactctaa tataagcaat tttcaaattt
     2821 attccctact tttctataac gtttataata caccctaatc atacgttaga actctaaatt
     2881 tttcaatgtt atatttttgt ctatttaggg taaactctaa atagacaaac ggtttttgaa
     2941 ttctaaaagt taaatagttt ataatagaag aaaaagaatg gggtgacgat ttcttagatt
     3001 gaaaaggaaa gataaatatt atagattatt acttttgaca gctgaagcag gatggatgga
     3061 ttttccagaa gttgtaaaag aagttcaaga gataggtgca actatttctg atagccgctc
     3121 ctctcctatg aactatcagg atgcccatgg aattaaaata attgatcaag agagaaatgt
     3181 cagaaaatac acaacaataa atgagtgtgt aattaatgaa aatctttgta gggcaccgat
     3241 aacgaaacac attaagaata gaagcaaagc aaaagacgga agaactttta ctaccttcta
     3301 agtttttttc atatttgttt gctatactta gatctaagga attagacttt cgctgcccat
     3361 tatttaagtt tgaggtagcg tcttgcgagg gatcaataat ggaagaagaa aaaatatttg
     3421 aaaaacgctg ggaattggct tcagttgaac aaagagcaag gtaccataat ctaatgtcct
     3481 cttaccgtaa tattgattgg acttacaaag aaaagaagta cttgctttgg ttatgtcagt
     3541 tagatgtaaa tacctttgaa acatttgaag tgattctaga taagattaaa aacagcaatg
     3601 aaaaaagagc tgatctataa tggttcgctc tttttatttt attaactctt tggttactat
     3661 atgtaaaata cagcttcata ttacaataaa tttcaggata caaatttatt taagcatata
     3721 taaatataat tgaaatagga cataaatata tgagtatgag aaaggtgatt ccatgtttaa
     3781 taaatgggca aaccacccaa tgaactcgag aggtagagat aatttgaagg agacggacaa
     3841 gttctgggaa gaagtatata gtaaaaacgg ttctgttgca aagttttcca aaaaatctat
     3901 tttagtgtaa aattgagaaa aaagacagag aggacagagt aatgaatcat tttaaaggca
     3961 aacaattcaa aaaagacgtc attattgtcg ctgttggtta ctacctgcgt tacaatctaa
     4021 gctatcgtga agttcaggaa ttgttatatg atcgtggaat aaatgtttgt catactacga
     4081 tttatcgttg ggtataagag tacagcaaag tcctctatta tctttggaag aagaaaaata
     4141 gacaatcctt ctattcatgg aaaatggacg aaacctatat caaaattaag ggacgttggc
     4201 attatcttta tcgtgcaatt gatgcggacg gcttaacctt agatatctgg ttacgaaaga
     4261 aacgggatac gcaagcagcc tatgctttct taaaacgact ccataaacag tttggtgagc
     4321 cgaaagcaat tgtgaccgat aaagcacctt ctcttggctc cgcctttaga aagttacaga
     4381 gtgtgggttt atatactaag acagagcacc gaactgtgaa gtatcttaac aatttaatag
     4441 aacaagacca tcgacctatt aaacgacgaa ataaatttta tcaaagtctc cgtacagcct
     4501 cttccacgat taagggcatg gagaccattc gaggaatata taaaaagaac cgaagaaatg
     4561 gaacgctctt cggcttttcg gtgtctactg aaatcaaggt attaatggga ataacagcct
     4621 aagatatttg gagttcagag agggcgcgtt tgattttcaa acttcgcaac agaaccgaga
     4681 accttgggtt ttcaagtgct ttttggttca ttaatcttaa ccatagtgtt tttaaagtgg
     4741 aaaacaagca acaaaaaaag tatgtggagc cttgtaggaa tagttcttat tatttttact
     4801 gtgactgcag caacagaagc aaattattcg cataaaattc agtcgtataa aggtcgttta
     4861 gttatgacca aaaaagatgt tacacgttat acatcctcaa gaactggagg cctatatgac
     4921 tatgcaccat tagatgcatt gaaatataaa gatcatttaa agaaacatga agtaaaagtt
     4981 ggtgatcggt gggagaatgc atctcataaa gcatataatt ccaaaatagt ctatacagta
     5041 gaagatgccg atgagcaaaa tatcacatta cctgtattca attattgggg aacagttgtg
     5101 aaagtaaatg ggaaaaagag cactatagaa aatgacaatg gtttagtgtc tattaaaggt
     5161 acggggaaaa gaactatagt tgaggtttca tctacgtatc caactacgat gattctatct
     5221 ttattaatta gttcaataac ttttatattc ttatttacac gttatttaat aagatgctgc
     5281 ggtgaaatga aaatagatta gatagatatt ataaatatgt tgattctatt ttcatagcaa
     5341 tggagaaaat acattagcaa tatataaaag aaggtatcaa gagtcacagt gaatttattc
     5401 cgaaaagtga aaagagcaaa tataaaattt aatgaaaata tagaactaaa taggaaaacg
     5461 tagacgtaat tttctacgtt ttccttttca tatatattaa atattacgga aaattatata
     5521 aactaattta gtgttttgtt tgtaattcct aattttgctc cacttttaga taatttttag
     5581 gtctatttaa ctcatcagac attctatgct catttttctc caaatttgtt tcaactcttt
     5641 cgtacttaat tttgcgtatt tgaaaattct tcttttagag tacttttatt acgatactag
     5701 tatgatatat atctatagca aacagttaaa atcttcctaa aataagcgaa tagcattcct
     5761 aaaacctata aaatttttag atgaagcgac aagtggacaa ttaataaact acttgaacat
     5821 gaaattatgg atcacctttc aaatttagaa aatggaattc ttatacttat tgcgcaacat
     5881 ttattgattg caaaattatg tgacaaaaaa aactagtaga agaaagaata atggcttgat
     5941 aataaagtac tacctattaa aggtagtact ttatttaatt tagtataaat taacatacta
     6001 ctatatttta ggtttcatat tatataaatt aataatatac taccatttgt tggtagtata
     6061 atttaaaggt aataagttat tcttttatta ctatgtttgg gttttatagc aaagaaagtt
     6121 taataagagt ttggacctag caaacaatat taatgaggtc attaaatctt attatcttta
     6181 gtgatataac gaaatatcta cacagacaaa atgatagcgt aaaatatttt gtgtaaatag
     6241 atatttaggg tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc acaaccgaaa
     6301 ttttatagga ggactttctc atgcccaatt ttactacaga aattatggaa acactaatca
     6361 ataaaggtga tttagatgaa ttatttcgat gtcatttaga attagctgtt aattcattat
     6421 tgcaggctga attaacagct tttcttgatt atgaaaaata tgatcgtgct gggttcaatt
     6481 caggtaattc ccgtaatggg aattattcac gttcattcag aacagagtat ggagaattaa
     6541 atttggtaat tcctagagat agaaatggta agttttctca acaaacttta cctgcttata
     6601 aaagaaccaa tgactcttta gaaaccacta ttattcagct atttcaaaaa ggaattacta
     6661 tgtctgaaat ctctgaattg attgaaaaaa tgtacgggca tcactatacg ccacaaacca
     6721 tttcaaatat gactaaaata gtttctgaag atattattgc ctttaaagaa aggtccttag
     6781 aatccagata ctctgtcatt tttatggatg ctactcacat tcctttaaaa agacaaactg
     6841 tttcaaaaga agcagtttat attgtaattg gcattcgatt agatggaacc aaagaggttc
     6901 ttgggtttag tcttgctcca actgaatctg cttatgtttg gaaagagatt cttcaagacc
     6961 taaaagaccg tggtttaaaa gaggttttat tagtcgtaac tgatggttta agtggcatta
     7021 acgatagtat tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat
     7081 ctagaaatat cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
     7141 aattagttta tcaagcaagt tcaaaagaag aagcaaataa tcaaataagg tttatgatag
     7201 ataaatggaa aaagcagtat ccacgagtgg ttaaattgct catgaatcct gctatattaa
     7261 ccttttataa tttccctccg tcaatcagaa gaactattta ttcaactaat ttgattgaag
     7321 gttttaacaa acaattaaag aaatatacca aaagaaaaga gcaatttcct aatgaagaat
     7381 ctctggagag atttctagta tctcaattca acaactataa tcaaaagttt ctatgccgag
     7441 ttcataaagg ttttaaagaa atacatgata cattagaatc aatgttttaa cttgatcaac
     7501 agaatgggct tttccattta cacaaaatta ttgacgctcc cgacaaaatt atgatcttgc
     7561 gtaaagttat aaaatattaa taaaaatatt ttattaatga aaagcttggt agcacggtta
     7621 ttcatatgct aatttatttc actttatagt aaggggagtt cttagtatat tgttcaaact
     7681 taaatttcat ataatttaat tatgtaaatt agctattcta agaacttcta cggataactg
     7741 cccgtctaaa atttaaataa gagcttagaa tcttcatata agtaaggtta atttttttgt
     7801 acaaaaaagt cggaatattc tgagttactg aaatgatata aaatcataaa ctaaacccct
     7861 tgattttctt cacttttaac agagtatata tataagttca tgattatggt aaaatatata
     7921 tataatcatg aactcagaat atttttaaga agggaaacaa tagtgagcta taatgtacaa
     7981 ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag aacaagtaat
     8041 ccagaaagag atagtttttt atttttgttt ggaatcaata atgggttacg tatgtcagat
     8101 attataggat taaaagttgg agacatacga ggtaaaagta agcctattat tgttgaacat
     8161 aaaacaggta agcgaaaacc gatttttata gataatttga gagaagagat tttattatat
     8221 actgaaggta aagaagaaaa tgattggtta tttccaagtc ggcaacaagg tcgacatata
     8281 acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg aagagatgat
     8341 ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa aactagagac
     8401 attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa aaggtacatt
     8461 ggtataactg aagatgaaat aggggcatcg ttacgtggtt ttaaattggg aggctaattg
     8521 agatattagg atatattttt tgtgtataat gaaaacttag aaggtgagat taatgaagat
     8581 aactgaattt tcggaagtat ttaaaacgtt tagtaacatt tacaatagaa taccagagat
     8641 agaacaaaat gttgtagttg ataatgatta ttatatagag aatgatttta ataaaccaag
     8701 aaaatatgac aacctataca attttataca caatagaaca acaataaata acaacagtaa
     8761 cactcgagtt ttgattaatt cagcatattt tcatattaaa tatttaaaga aatatgagta
     8821 tgaagaagtt gtattagatt atgggctaaa tagacagtca tttttaattt atgcaataaa
     8881 taacggaaat ttgccaagca ataaaaagaa cataattatt gaaacatatg cctatctgga
     8941 ggggggatct aaaaaactaa ttaagtcatg taatatagag tatcaattat cagtaggaga
     9001 aattaaatgt gttttcgaag aagatttaga acttgactta aaaaaaattt ttgccagcat
     9061 tcatgattat ataggtttga
//
LOCUS       DAZUMT010000110         9043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000110 DAZUMT010000000
VERSION     DAZUMT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.112"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..540
                     /locus_tag="ACG8FM_002440"
     CDS             97..540
                     /locus_tag="ACG8FM_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5095059.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="ACG8FM_002441"
     CDS             complement(546..1730)
                     /locus_tag="ACG8FM_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS5095060.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="ACG8FM_002442"
     CDS             1998..2696
                     /locus_tag="ACG8FM_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5095061.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="ACG8FM_002443"
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="ACG8FM_002443"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS5095062.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3506..4039)
                     /locus_tag="ACG8FM_002444"
     CDS             complement(3506..4039)
                     /locus_tag="ACG8FM_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095063.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="ACG8FM_002445"
     CDS             complement(4336..6717)
                     /locus_tag="ACG8FM_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5095064.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACG8FM_002446"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACG8FM_002446"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS5095065.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="ACG8FM_002447"
     CDS             7418..7957
                     /locus_tag="ACG8FM_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS5095066.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="ACG8FM_002448"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="ACG8FM_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS5095067.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="ACG8FM_002449"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8FM_002449"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
BASE COUNT         3009 a         1589 c         1838 g         2607 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttt
//
LOCUS       DAZUMT010000111         9012 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000111 DAZUMT010000000
VERSION     DAZUMT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.113"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..1134)
                     /gene="recA"
                     /locus_tag="ACG8FM_002450"
     CDS             complement(85..1134)
                     /gene="recA"
                     /locus_tag="ACG8FM_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS5095068.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1243..2484)
                     /locus_tag="ACG8FM_002451"
     CDS             complement(1243..2484)
                     /locus_tag="ACG8FM_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS5095069.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2724..3302)
                     /gene="pgsA"
                     /locus_tag="ACG8FM_002452"
     CDS             complement(2724..3302)
                     /gene="pgsA"
                     /locus_tag="ACG8FM_002452"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS5095070.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3511..4434)
                     /locus_tag="ACG8FM_002453"
     CDS             complement(3511..4434)
                     /locus_tag="ACG8FM_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS5095071.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5172..6458)
                     /locus_tag="ACG8FM_002454"
     CDS             complement(5172..6458)
                     /locus_tag="ACG8FM_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5095072.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6451..7719)
                     /locus_tag="ACG8FM_002455"
     CDS             complement(6451..7719)
                     /locus_tag="ACG8FM_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5095073.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7971..8432
                     /gene="mscL"
                     /locus_tag="ACG8FM_002456"
     CDS             7971..8432
                     /gene="mscL"
                     /locus_tag="ACG8FM_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS5095074.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8491..8937)
                     /locus_tag="ACG8FM_002457"
     CDS             complement(8491..8937)
                     /locus_tag="ACG8FM_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5095075.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         2480 a         1955 c         1606 g         2971 t
ORIGIN      
        1 cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac tcttcagacc
       61 agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt cttgtccttt
      121 ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa ctagagcaga
      181 aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt cccgtccttg
      241 accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa tgtctttttc
      301 tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca tgacatctac
      361 ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct ttgtacggtt
      421 accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca aacggatcgt
      481 tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa acatcacgcc
      541 tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc
      601 agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat gtgagtcacc
      661 catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga ctactacgat
      721 atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc
      781 aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat attgcggatc
      841 taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta cttctgcaat
      901 agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt cgatgatacg
      961 tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc cacttggaat
     1021 tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt taccatagct
     1081 cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg ccaatacgat
     1141 aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat atctttttta
     1201 gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc ttaaaagctg
     1261 ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag cacgatctct
     1321 ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga tccatactgt
     1381 tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag ctacactgta
     1441 atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt ctttgctgac
     1501 tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt ttgtttggag
     1561 cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt cgcctaatgc
     1621 ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct tgttttcttt
     1681 cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat aaaaatattc
     1741 ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac
     1801 atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat atggtgcgat
     1861 cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc gcgattcacc
     1921 aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa aacgttcctc
     1981 tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg gaccgggaag
     2041 aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt
     2101 tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt tggtcatttt
     2161 tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat tttgtatgag
     2221 agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa
     2281 tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca actcttctaa
     2341 acgctgctga ttatctccta caactgtttg ataataaact tcgataccta gatcagctag
     2401 ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa gttctgttcc
     2461 gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata cgctgtattt
     2521 ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg tcacagctcc
     2581 ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata
     2641 aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc
     2701 acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg
     2761 aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct
     2821 actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc
     2881 atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg
     2941 acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc
     3001 caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta
     3061 tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt
     3121 tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt
     3181 gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaacctaca
     3241 accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc
     3301 acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat
     3361 tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg
     3421 aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa
     3481 gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac
     3541 attttttgga ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt
     3601 gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa
     3661 cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac
     3721 tagcacacct aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc
     3781 gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt
     3841 cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct
     3901 ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc
     3961 tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat
     4021 cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg
     4081 cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc
     4141 agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc
     4201 ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa
     4261 ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg
     4321 ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat
     4381 ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg
     4441 ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt
     4501 tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat
     4561 ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga
     4621 cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa
     4681 atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac
     4741 aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt
     4801 aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
     4861 ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata
     4921 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
     4981 tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag
     5041 agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa
     5101 agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca
     5161 taaatactgg tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat
     5221 gcttccccag ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca
     5281 aataaagttc gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa
     5341 tttaatgatt ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct
     5401 gacacttccg gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc
     5461 tctggttcgt ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat
     5521 tcaaaaccaa agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa
     5581 gtattgccta aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct
     5641 gttccttctt gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta
     5701 atggctgctt ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt
     5761 acaatttctt gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc
     5821 aatttctcag gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac
     5881 gtccgatagc acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc
     5941 gcaatatcga tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc
     6001 cgccagttag ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg
     6061 tttacagttt cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc
     6121 ttttctacct gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca
     6181 ttggcggaag ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac
     6241 aacttatgtt ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca
     6301 tagggaataa attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg
     6361 tgatagtcat ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa
     6421 agtgtttcat tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa
     6481 gaagattgcc tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct
     6541 ctccatacgt ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa
     6601 aaaagaagct tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag
     6661 cattgctttt gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa
     6721 ttgctcgtga atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac
     6781 actcatgaat cctcggaacg tatccacgct actagaggca taataagcta gactttcttt
     6841 ctctctgaca ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac
     6901 cattagcgca aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag
     6961 ctttgcttga acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg
     7021 ggtataaaac agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc
     7081 ttcagctgct ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc
     7141 catcatttct tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac
     7201 tacgcccaac cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc
     7261 ataggaagca aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt
     7321 cttttccaag tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa
     7381 aacctctttt aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc
     7441 attcacaaaa ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa
     7501 tccaaagctt gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccggata
     7561 attcaaacta tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg
     7621 ttgcagacgc gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa
     7681 gacatgaaga ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca
     7741 gttgattgcc cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct
     7801 tgaaatttat aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta
     7861 tagcatagat gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat
     7921 tttttatttt tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag
     7981 aattcaaaga gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg
     8041 gtagtgcgtt tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag
     8101 gttggtttgt cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg
     8161 actggtcgcc gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta
     8221 catttctgat cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga
     8281 atttacgctc caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc
     8341 ttagtgacat acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag
     8401 agcaaacaaa tctttccgat acttctaaat aataaagaat ctaaaaaaaa gaaagccgat
     8461 tttcagtgtc ggctttcttt ttttagattc ttaacttgct acttgttcct ttttcatgtc
     8521 gatatgtgga atatcatctt ccaaatacac atctgagatt gccttgaatc caaacgactc
     8581 ataaaaagct tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt
     8641 ctggatcatg ttaatcgttt cttgaacaat ctttcttcct aaaccatctt tacggttgtc
     8701 ctgatgaaca aggacacgtc caaaatggat actctccgct tcttcatata ctcgcgtgta
     8761 ggcaaggatc ttcccatcag tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc
     8821 atcaacttct tgataaggac actgttgttc cacaacaaaa actgcgattc gttctttgac
     8881 gatcgcgtaa aactctgctg ttgacaattc ttcaaatttc tttacctgat attccactaa
     8941 gcaaacgccc cctgcttttt tattcttagc ataccgattc gcacacaaaa aagcaaacga
     9001 accttacgaa tc
//
LOCUS       DAZUMT010000112         8803 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000112 DAZUMT010000000
VERSION     DAZUMT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.114"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8FM_002458"
     CDS             <1..1087
                     /locus_tag="ACG8FM_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5095076.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1294..2436)
                     /locus_tag="ACG8FM_002459"
     CDS             complement(1294..2436)
                     /locus_tag="ACG8FM_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5095077.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2441..2626)
                     /locus_tag="ACG8FM_002460"
     CDS             complement(2441..2626)
                     /locus_tag="ACG8FM_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5095078.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2980..3225)
                     /locus_tag="ACG8FM_002461"
     CDS             complement(2980..3225)
                     /locus_tag="ACG8FM_002461"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095079.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3219..3620)
                     /locus_tag="ACG8FM_002462"
     CDS             complement(3219..3620)
                     /locus_tag="ACG8FM_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5095080.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3807..5615)
                     /gene="uidA"
                     /locus_tag="ACG8FM_002463"
     CDS             complement(3807..5615)
                     /gene="uidA"
                     /locus_tag="ACG8FM_002463"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS5095081.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5638..7404)
                     /locus_tag="ACG8FM_002464"
     CDS             complement(5638..7404)
                     /locus_tag="ACG8FM_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS5095082.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7417..8211)
                     /locus_tag="ACG8FM_002465"
     CDS             complement(7417..8211)
                     /locus_tag="ACG8FM_002465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5095083.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8221..>8803)
                     /locus_tag="ACG8FM_002466"
     CDS             complement(8221..>8803)
                     /locus_tag="ACG8FM_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5095084.1"
                     /translation="PDYYNDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASV
                     GMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQ
                     REDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPS
                     ICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
BASE COUNT         2857 a         1687 c         1169 g         3090 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa tccatgtaaa catggtttat
     1141 tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc
     1201 gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta
     1261 tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt
     1321 tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat
     1381 agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa
     1441 ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc
     1501 tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat caaaagaaaa
     1561 aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt ttaaatcttc
     1621 aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg tttcagcaaa
     1681 ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc tgccatttat
     1741 taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac ctgtaacaaa
     1801 aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat aaacactatt
     1861 atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct gagtttttat
     1921 tttacctata gtatctacag gattttcttc aataatttca agtacaattg cccttttaaa
     1981 aactactctc aacttattga atatctgttt aatataattt ggtgataatg gttgctcaat
     2041 gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta tatgaattgg
     2101 tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta aaatatgatc
     2161 tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa aatactctcg
     2221 ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag aaaaactttg
     2281 ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc ttctggtcac
     2341 ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc aagtgcctct
     2401 ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc caagaaatat
     2461 ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta cgataaaaaa
     2521 agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta gcatagtact
     2581 gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata gcaccttcct
     2641 tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg aatttcacct
     2701 ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta aatcatcgct
     2761 ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac agcaagaaat
     2821 catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat ctatttaatt
     2881 ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac tattagaaga
     2941 ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt taaattttaa
     3001 gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta cataggtaac
     3061 gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat aagaatgata
     3121 atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata agataatgga
     3181 tagtggaact aataggtact gccgcttttt agatttatct accataggtc ataccccatt
     3241 ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct catattgaga
     3301 attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat aatctttctt
     3361 cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat taatatttcc
     3421 attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata cctgccttca
     3481 tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt tcgaatagac
     3541 ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg tatagatatg
     3601 tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac taatacaaca
     3661 atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa cataaaaata tttatcattc
     3721 tttaaacaga aaatctttct ataaaaaaca aaaaaaaacg attaggctag gctaatcgtt
     3781 ttttgaacat tcttaaaaaa tccaaattat tttttataac tgaaattggg tattgatgac
     3841 catcgtttct gtagatagta agcaatcatt ttaggttttc tatctctagt aaagattcct
     3901 ttcttatttc cttgcactct taaaatccct tgacttgttg caaaatctgc aaaattccac
     3961 acttgttcac caacaaaatt ttcacatgta tctattactt cgtgatttgc ttcgtaataa
     4021 gccacttgat actcttctgt aaacattgtt ggtactgtat catgaaatcc agctactgta
     4081 tctgctccat attctgtaaa cattattggc gtatcaggac atctttccat ccatccatct
     4141 agctcttttt tcaacagata ttttgcatgt gccagatccc ctcccgaaac ataccaacca
     4201 taataacgat ttaaacataa aacatcgaca atatctccta ccttacaaac atctggcgtt
     4261 gattttagat aagtaacgat tgttacaggt cttttctgcg gatcaagttc tttagttaat
     4321 tcaactaaag gttcaaaata ttctttagca ccttcggaat ccgaatctgg ttcatttgca
     4381 atagaccaca ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc cttgatcacc
     4441 gttttatgag aattttctgt tcgtatctct ttccacgtat tatgtttttc cacattatca
     4501 tctaataaag tggccatgaa atttaaatgt aaccctactg caggtacttc atcaataact
     4561 acaatacctt ctcgatctgc taatcgcata atttcttctg agtaaggata atgtgatgtt
     4621 ctaaaagaat tagcacctat ccatttcatt aaattaaaat ctttaatatt tacagcctca
     4681 ttcaaaccac gtccattaac atacgaatcc tcatgcttac caaatccttt aaaataaaaa
     4741 ggtttattat taatcaaaaa tttgccattg gtaacctgaa cactgcgaat accaaattct
     4801 tctgtatagg tatcgatcac tttattatca tcaattaaac taattttcat actatataaa
     4861 taagcgtcta gaggttccca aaaaattgga ttcttaagct gaatcattcc tttctttcct
     4921 tcagcttcag ctacaagttg agcctcttcg tcatatatcg ctattaagat ttttccggtc
     4981 cattctgtat cgatattgaa ttctacactt gcatccaaat cagttattct tgtattaata
     5041 gtaatatcac ttatatagtc tttcggagtt gtatataact taacaggtct atgaatacct
     5101 gcataattga agaaatcaaa attaactaag ttttcctttt ttatatcccc attttcctgt
     5161 actgtttcct tcataatccc cacaggtaaa gtactatcgt caataatatt gtttacagca
     5221 actgtaattc gattattccc gtcttttaca aaccgattaa tttcagactc aaatggtgta
     5281 aacccacctt tgtgttgagt aataaacttt ccattcaaaa aaacttttgc ttcatgtgtt
     5341 gcagatccaa aacgaagaac taatctttct tctaataatc ttttaggaag agtcacttct
     5401 ttttcatacc acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta
     5461 taagaagagg gaactgaaat cggataactc tctttttcat ttattggttc taaagcaagt
     5521 tcttcctgta agtcatcatt tgtatctaat ttaaatctcc agagaccgct taagtcaatt
     5581 acttgcctcg tctctgtcat aatgggatac aacatataaa ccactcctat ctaaatatta
     5641 aataaatgta tcctttaaac cacaataagc atctattgga cttactccct taaattcaga
     5701 aagacccatt agtttgtcca ccaatatttc taaagtttct tgttttccat cataagtatt
     5761 aatataagtc tttacttgag gtacatcagc caaatgaaac ggacactgta cagatacaaa
     5821 aattgttgga atttcatgaa tgtaaaacgg tatatccgga gttcctttac ttgctggcca
     5881 gacaattcgt tgaactgtgc ctggattaac attagctata ttaatgatca agtcatattt
     5941 tttagttaaa tttttaattg gtctcttagc agcatatatt ccagctatat ttttaatttt
     6001 ttcttcttgc gataatcctt tcaattcttc ttcaggtgaa atgtattttt caacttcaaa
     6061 tccttttgct gacaaaatct gtttcaataa atcaatggct tgaggtctat ttccagcaac
     6121 tacttcacca aacccacctt ttaccccatg tacatctact agtaaaatcc ttttatattt
     6181 ttctggtaca ataggccaaa tatcttgttt atctttaact aatgtaattg cttggtcagc
     6241 cacctcttta aaaattgatt tattttcaat taaaccaatt ttttccagag cttcttgctt
     6301 aggtaataaa atttgttctt ttgctttttt gtgtaatccc aatttagctt ttaaccctaa
     6361 aattcttttt aaagcatcta ataaccgatc atcagtgata attcctttac gatagccttc
     6421 catcatataa ttaaagtctt catctggatc attaaaaaat agaaatagat cacatccagc
     6481 tgcaatagaa gtgggcaata attctgatcg tttcattgcc ccagtcattc ctaacatatg
     6541 actagcatca gtaactacta atccattaaa tccgagtttt cctctaagta agtcagtgat
     6601 taattctttt gataaagttg caggaagtaa atcttcatct ttcagatcag gattaaaatg
     6661 ttttgaataa gctggcatat ggatatgacc tgccataatt gaaggtaatc cagcttctat
     6721 taagcctgag tatacttttc caaaagaatt atcccattct tccgtaccta aactattaat
     6781 actaaaagat agatgctgat ctcgttcgtc aataccgtca ccagggaaat gtttagctgc
     6841 aggcaaaatt ccactctcct gaataccttt catataagct aaactaagtt ctaaaacttt
     6901 atcagggttt tgtccaaaag atcgactaga aatgatagga tttctccaat ttctattaat
     6961 atctacaata ggagcaaaac tccagttaca tccaatagcc gatgcttcaa tcccagaaac
     7021 tctccctaat tcataagcat atttcgcatc atttgttgca ccaattttta cttctacacc
     7081 aacttcagta ccatctgtac aagctccgtt accacctgct tccgtattag cagcaattaa
     7141 tagaggaatt ttactttctg tttgtaagat atagttttga tcatatacct catcagcagt
     7201 tcctggatta tatcgtacag ctccaatatg gtactgattg accatattac tcaagtaatt
     7261 tttatcacga ctagatccca tattaacaaa caattgacct attttttctt ccaaagtcat
     7321 agaatcaatc gtctcttcaa tccaagaaat agcatcttca tctaaattat atggtttttt
     7381 tcgtaaatct acttttatag tcattttctt tctcctctac ttattagtaa gatttttcca
     7441 aaaagtatct atatattttt gttcaatatc tacataatct cccgataaac atttaaataa
     7501 aacatcagtt cttaagtttt cccatgattc tttttcttta ttgaccaaaa taggataaaa
     7561 gaaaacttta tttttatttg cagcttctag atcaccagga gaatcaccaa tcattaatat
     7621 cttatccaaa tcacaaccat tttcaattac acttcgaata atatcttctt tttttccttt
     7681 atcttgacaa cacagttcat caactaatga taataattca tgtctttccc attcttcttg
     7741 aacagcttcc ttattagctg agcttacaat aattatcttc ccatattttt tcagagcttt
     7801 tatagtatct ctagcactta aaaagggttt atctaatcct tttgcttgac taatctgttg
     7861 attgacttct tccgaccatt ttaaagcttt taataaatcc tctgactgag aatattctat
     7921 ttccctcttc aaagatggat tagacaattc ttttgttaga gatacccagt gctttaatcg
     7981 attaatcgat gttacatcat ttttttctaa tgttttaact aaacctaaaa atctgtttat
     8041 tccacgagtc tctgaatata aattaatatt attccaatcc tgtaggaatt tttctctatg
     8101 atgaacatca aaaatctctg cagcaattgg tccgaaaaag agattgtgtt tataagtcat
     8161 cgtatccata acacagccat ctgaatcaat acaaaatatc caattattca tatttctctc
     8221 ctatttatgt ttttgtttta aaagctcgca attttctaag actttatctt tagataatgg
     8281 ataaaggaaa aataaaatta atcctacaat cagaaaacaa attgatggaa ttaatgttga
     8341 agcgttatat aacttttcta aaacatctcc cgtttgtaca tttgctaaag agtcatagcc
     8401 tataatagaa agagtccaac ctccgacgcc tccagctaga gcttgaccta tttttctagc
     8461 aaaagaatat accgcatata cggtcccatc ttctctttga tcagacttta cttcttgatc
     8521 atcaataaca tcagtaatat ttgcccaaat aacagtatta aacacaccca aacccatata
     8581 gccaaccgaa ctaagaatta agaatacata catattacta gcttttatca aaaataagat
     8641 caaatatact attgctgaaa agaacattcc tactgatgcc agttcttttt ttccatattt
     8701 tttacttaaa aacaaagtta aaggcgaaac aactattaaa accaaaatag gattgataaa
     8761 attcattaaa actattccct gtgcatcatt atagtaatca gga
//
LOCUS       DAZUMT010000113         8738 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000113 DAZUMT010000000
VERSION     DAZUMT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.115"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..768
                     /locus_tag="ACG8FM_002467"
     CDS             121..768
                     /locus_tag="ACG8FM_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095085.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(861..3140)
                     /gene="helD"
                     /locus_tag="ACG8FM_002468"
     CDS             complement(861..3140)
                     /gene="helD"
                     /locus_tag="ACG8FM_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS5095086.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3418..5241
                     /locus_tag="ACG8FM_002469"
     CDS             3418..5241
                     /locus_tag="ACG8FM_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5095087.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5288..5467
                     /locus_tag="ACG8FM_002470"
     CDS             5288..5467
                     /locus_tag="ACG8FM_002470"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS5095088.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5804..7744
                     /gene="thrS"
                     /locus_tag="ACG8FM_002471"
     CDS             5804..7744
                     /gene="thrS"
                     /locus_tag="ACG8FM_002471"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS5095089.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7968..>8738
                     /locus_tag="ACG8FM_002472"
     CDS             7968..>8738
                     /locus_tag="ACG8FM_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5095090.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYM"
BASE COUNT         2732 a         1561 c         1866 g         2579 t
ORIGIN      
        1 acagattgtc ttgctttttt gtgtgattta ttttcttttc taattcaacc tgtggtatga
       61 tgagagcata agtatcatgt gaaatgatat gaagtatgtg gaagatggac ggggaagaat
      121 atggaagcaa aaatcacttt agaacctttt gaacgtatct taagcggtta ccgcaaagta
      181 gaagagttag ctgtgaacgt aactgattgt tcaaaattag cgcaaaaata cgcaagattc
      241 ggcgttgaag gctatcgatt aggaaactat gtcggtacag ggtatctgaa ccgttattta
      301 gagtgtatgg tggaccgcgc accaatgctg atttatagac aaaattatct gattccgctg
      361 ctttttcgtc gttctgattc agcctttcgg ttatttgaag aagagtatcg gatggaggct
      421 ttctttctac tattagagtg gagcttgaaa catcgtcctg aaaaaattct tatcgaaaga
      481 aatgagaaga tagacacgaa aaaaaataaa gtgatcgata gtgcatacct agcatttcgt
      541 gtatcagaga ttttggattg tggcggctat ccaatcagca atttccaatc aattgaccaa
      601 ttcattgaat ggaatcgaat ttaccgattg atcgacaatg gcgggattgg ccgtcatagc
      661 aaggtatttg acccagaata tccagaaaac atggaagaac tgaaaatgat tatttcgctt
      721 gtcaaattaa aatatcctga aacagatttg gatttatata tagaataaat gaccttaaat
      781 gattgaatta ctttttctgc aattattatt gtaaataaaa agacaactgc taacattaat
      841 gatagcagtt gtctttttac ttacagtact tttttctcat aagatgaaat atccatattg
      901 tttaacaaag gactttcatt attggaggta aaaagggtca attcatgcat cgcacgtgta
      961 cacatggtgt aaaggatcaa tttgtcttgc tgagaagaaa aatgttctgc atctacatcc
     1021 cacaagaaaa ctcggtcaaa ctccagacct tttgctaaaa aggctggaag taaaataatg
     1081 gaacgcttca taaagtcact ctcatttaaa atcagctgga cttgttgttt caactcgtct
     1141 ggcagttgag cataaagagc ttcgcattct tcttgtgttt tagctatgat tgctgtacgc
     1201 caataccggg cttttccctg agtcgttcga atggtatctg ctaaccaatt caatttgata
     1261 tcatctgttg ttccttggat gagcaaagga aggtctcctt ttcttgctgt tgtttctact
     1321 tgatgttctt tcacaataaa atgattagca aaatcagtga tttcttttgt ggagcgatag
     1381 cttgtcgtta attggaaacg agtgacttca cggtccacga aaaggtcttc caatgatcca
     1441 acgatcgttt catttccaaa gacattttga tttagatctc cacagaaagt caaatttgct
     1501 tttgggaaca aggcttgcag caatgccact tgtgatggtg cgaaatcctg catttcatca
     1561 ataaagatat agcgagcttt gtgtgtaacc gtcactggat atgttcggcg catcagtaaa
     1621 aagaatagta cagcatcctc ttgacgcaat tctctttgac gcaagtgtgt acgaacagtt
     1681 gtcaaatgtt cctgccacct ggtctcgctg attttatgtc gtgttagaag agaaagtggc
     1741 acagtttgta gaaagtggag atattgtttc gttatattga caaattgata attcatgatc
     1801 cctcgatgga ttcgacggaa tcttttacgt acgatttggt tggccagttt tttacgtaat
     1861 gaacgctcct tttgttctgt atattcaaga tttggatctg ttgcgtatag ttcttggagc
     1921 tgttcttccg ccagatcttt gacccattgc tgacgtgttt cgtctttttg cagaccgcct
     1981 aattttttca ggagcttcgt ttgtaataga gaaagtctat gatgtaaagg taattgttca
     2041 tttgtttctt tataccattg gcgaatcgat tcctttgata ggatcgtacg gccatttatt
     2101 ttcatatcac ggaacaaggg accgtagttt gtgatgcctt ggatatagcg atcaatctga
     2161 tcgactaagg tcaatccgct tttcatcact tgtatcggat ctttttcccc tagcaaaaaa
     2221 ccactttctt gttgctgttc atctagtaaa gaaaactctg gcagcagctg caccaaataa
     2281 tttttgaatg tctgtgtagg cacgccgctt tcacctaatg aaggcaatac agtcgagatg
     2341 taatcagaaa acaaatggtt gggtgaaaat agaagaacgt tttcagcctc gagccacttg
     2401 cgattatgat acaacagaaa agcaatacgt tgaagcaaag cggatgtctt tccacttccg
     2461 gcgattcctt caatcagcat tactttactt gttgtgtccc gaatgattgc attttgtgct
     2521 ttttgtatcg tagagacgat gtttttcata tggttgcttg atgattcatc caatatctct
     2581 agcaaaaagt catcattgat tacttccgac gtatctacca tagataaaat acgtccttct
     2641 tggattttaa attgtcgttt caactgcaga tcgacatcta ttctatctgt ttcagtttca
     2701 taaaatgcgg gaccaatctc accttcataa taaagattgg caattggtgc gcgccagtca
     2761 ataacgattg tttcttctgc cagatctctt aaagaagcga tacctagata taatgtttct
     2821 gtttcacggt cttctttaaa gtcgatacgg gcaaaataag gactatcttt catgacagac
     2881 aacgtttgca ggcgtttttg ctgtgcttca agagtatggt atttcaaaag caattcctgc
     2941 tcatgttggc gatattcaac agcagattca taaaaagctt cattggaacc agctcgaatc
     3001 ttgtgattag ctgtgtcttt tgtcgaatga ttcatttcct gagtcaattc ttgttgtttt
     3061 gtatccagca tattttgttc gatctggatt agttcgatcg tttcatccac gtgcgtttgt
     3121 tctagttgtt tctcattcat cgcatgtttg ctccttccgc cgtaaataac gaattaaaag
     3181 tatagcatag tttttcgaaa aaaggtactt tttaactgat gaatcgaaaa agtaatcctt
     3241 gctgaatatt tttgtttgta gaactgatga attttggaaa cgctgatagt gatagttgtt
     3301 ttcaattaag gcctattgtt ttaaaaagtg ctagtcaagg actatgctta agatgaaaga
     3361 ggcagctgga aatttattac taacctctct aagtcacata aaagaggaga aaaaacaatg
     3421 acacatttca aaaaattttt acttactttg cttattggtg ggatagcttt attaagtgaa
     3481 tttgtttttc accagccggc ccttgcattt gcgattgtcg catttactgg agggattctc
     3541 gccttcttga tgtttttgga aatgatcaag acgttacgtt caggaagata tggcgtagat
     3601 gtattagcga ttaccgcaat ccttgcaaca ttagccgtca aagaatactg ggctagtttg
     3661 atgatattga tcatgcttac gggcggagat agtctagagg attacgcgaa taaacgagcg
     3721 ggtaaagaac tgcagtcttt gttagataac accccaagaa ttgcccatcg attcaaagaa
     3781 ggaaaattgc aggatatagt aatcgatgaa ataaatattg atgaccagtt actagtcaag
     3841 cctggcgaac ttgtacccgc agatggtcga gtgaccaaag gagaatcttc ggtcgatgaa
     3901 tcttctttga caggtgagtc ccgcccggaa accaagaaac ctggagatca gattatgtca
     3961 gggtctttaa atggagacgg atcgatccaa atgatcgctg aaaaacgtgc cgcagacagc
     4021 cagtatcaaa tgattattaa actagtcgaa gaatcaaaag aaaagcctgc ccgatttgtc
     4081 cgtttagctg atcgttatgc agtccctttc acgttgattg cttatttgat cggtggtatt
     4141 tcttggtggt tctcaaaaga ccctttacga tttgctgagg tactggtagt ggcatcacct
     4201 tgtccactga ttctagctgc gcccattgct ttagttgcag gaatgagccg ttcaagtaaa
     4261 aacgggatcg ttgtaaaaac tggaacaacg attgaaaagc tcgcacgtac aaaaacgatt
     4321 gcttttgata aaacaggaac gcttaccaaa gggatattgg aagtagaggc gattcagcca
     4381 gtcaatggaa taaaaaaaga agagctactt ctgtatgcag ctagtgcaga acaggaatct
     4441 agccatatct tagctcgttc gttagtgaac aacgtgaaaa acagccaact gtatcctgtt
     4501 agtcaactga aagaagtgac cggtcaagga atccaagcag tagtcaataa tcatgaagta
     4561 aagattggac gtgcctcttt tgtttcagct gaagaaatag aaacgtccaa tacagcaatc
     4621 tatgtaacaa ttgatcgatc ttttgcagga acgatcctat tttcggatca gattcgccca
     4681 gaagcagcaa agacaatcaa ggagctaaga aaacttgatg tgaaaaattt gatgatgatc
     4741 accggggatg gatctgccat tgctcaaacg attgcagaaa aagtccaatt agattctgta
     4801 catgcccgta gcttgcctca agataaactg accattttag aaagtatccc taaagatcaa
     4861 agacctgttg cgatggtagg agacggcgtg aatgacgcac cagcacttgc tgctgcagat
     4921 atcggtattg caatgggggc tcatggttct acagctgcta gtgaaagtgc agatgcggtc
     4981 atcttgaaag atgatctctt gcgtgtagca aaagcagtag tgatttcaag agatacgatg
     5041 agaatagcga aacagtctgt actgattggc atctttattt gtattttttt gatgttaatt
     5101 gcaagtacgg gactaatccc cgcattattc ggagctgttt tgcaagaagt cgtggatacg
     5161 gtctctattt tgagtgcctt gagagcaaaa aaagatccag ccatttttcc tgattcagta
     5221 cccaagacaa acatagtatg atagaatcga gttgaaaaaa gaacgatcag aaagaggaga
     5281 gagcattatg ccattcgtac atgtcgaatt agtagaaggt cgcagtccgg aacaattaga
     5341 aaatatgatg aaagatatca cggaagctgt tcataaaaac acgcaggcac caaaagaaca
     5401 catccatgtc atcatcaatg agatgaaaaa aggaacatac ggtgtaaatg gggaatggaa
     5461 aaaataagtt tagtttccca cttgattttt ctatttatta gtggttaaat aaacacataa
     5521 ttgattgatg agaaagccaa ctgaatttga gagtcttcga taagagagga aaagctaggc
     5581 tgaaactttt ccaggagaaa accaaaggat gaccactttc gaaacccttg ttgaagcaag
     5641 ggcggtcgga ccgttacaac gcttgaggaa cgcatttgcg tttgaacatt aggtggaacc
     5701 acgaactttt cgtcctagta tctcttttca agagatgcta ggattttttt gtttttcagc
     5761 ttgatgatct gatcaatcaa aacatttcaa ggaggaaact attatgataa aaattacttt
     5821 tccagatggt gcagtcaaag aatttgagtc tggaacaaca acattggcaa ttgctgaaag
     5881 catttcaaaa agcttagcaa aaaaagcttt agctggtaaa gtgaacggaa aactcgttga
     5941 tttgactcgt ccaatcgagg aagatgcaag tattgagatc atcacaccag accatgaaga
     6001 tgcacttggc ttagtccgtc attcagcagc tcatttgatg gcccaagcca tgcgtcgcct
     6061 ctatccaaat atccactttg gcgttggacc agctatcgat tcagggtttt actacgatac
     6121 agataatgga caaaaccaag taactgcaga agatttgccg gcaatcgaag cagaaatgat
     6181 gaaaatcgtc aaagaaaatc tgccgatcga acgccgtgtt ctttcaaaac aggaagcatt
     6241 agaaattttt gcaagtgatc cgtacaaagt agaactgatt agtgaattgc cagaagaaga
     6301 agtgattact gcttatcaac aaggcgaatt catcgatttg tgtcgtggac cacatgttcc
     6361 atccactggc cgtatacaag tatttaaatt attatcagtg gcaggtgctt attggcgtgg
     6421 aaactcaaac aatcaaatga tgcaacgtgt atacgggaca gccttttttg acaagaaagc
     6481 tttgaaagaa tatatccgtc tccgtgaaga agcaaaagaa agagaccatc gtaaactagg
     6541 aaaagaactt gacttgttca tggtttcccc agaagtaggt tctggtttgc cattctggct
     6601 tccaaaaggg gcaacaatcc gacgcacgat cgaacgttat atcgtagaca aagaagttag
     6661 tctaggttac caacatgtat atacaccaat aatgggagat gttgagttgt acaaaacatc
     6721 tggacactgg gatcattatc aagaagacat gttcccgcca atggatatgg gagatggcga
     6781 aatgcttgtt cttcgcccaa tgaactgccc tcatcacatg atggtctaca aaaacacgat
     6841 ccattcttat cgtgaattac caattcgtat cgctgaatta ggaatgatgc atcgctatga
     6901 aaaatcaggt gctttgtcag gtttacaacg tgtacgagaa atgacgttga atgatggtca
     6961 tacatttgtt cgtcctgatc aaatcaaaga tgaatttaaa cgtacgcttg aattgatggt
     7021 ggcagtatat gctgacttca atattacaga ttatcgtttc cgcttgagct atcgcgatcc
     7081 aaacaataca gataaatatt ttgatgatga cgcaatgtgg gaaaaagccc aagcaatgct
     7141 gaaagccgct atggatgaat tagaactaga ttactttgaa gcagaaggcg aggcagcctt
     7201 ttacggacca aaactagatg ttcaagtcaa aacagctctt ggtacagaag aaacattatc
     7261 aactatacaa ttagacttct tattgccaga gcgctttgat ttgacatatg ttggagaaga
     7321 cggggaaaat acgcatcgtc cagtagttat ccaccgcgga attgtttcta caatggaacg
     7381 ttttgtcgct tatttaacag aagtatacaa aggcgctttt ccaacatggt tggcacctat
     7441 acaagcaaca atcatccctg tttctgttga tgcacatggc gattatgctt atgaaatcaa
     7501 agaacgtttg caaatgaagg ggttgcgtgt agaagttgat gatcgtaatg aaaaaatggg
     7561 ctataagata cgtgcctctc aaacacaaaa gatcccttac caattagttg ttggagacaa
     7621 ggaattggaa gatgcaacag tgaacgtacg tcgttatgga agtaaagaaa cagctgtaga
     7681 agatttgaat atctttattg atgctatgga agcagaagtc aaaaattaca gcagagaaaa
     7741 ctaataggta aatttcctgt aaaaaagtag tagatagaaa gcaatccgaa caatagataa
     7801 caggtattcc tcaaagacac agatgctcaa tagaatctgt gggattgatg ggataagata
     7861 ttctgttggg cggattgttt ttttgttttt tatccagtat acgaccgtga ataagggtct
     7921 gatatggtat acttacatag actatcatta aaagggtgga ggaacttatg agcgtaatta
     7981 gtcgtattga aagtatgatg gaagattatt cttcagcaga aaaaaagttg gccaactaca
     8041 ttatcggtaa tattgagaag gtccctacaa tgacagctaa tgaattagca gaagcttccg
     8101 ggttgagtgc gccgacagtt gtacgttttt ccaaaaaaat cggttttcaa agtctgacgg
     8161 acttcaaaat cacgatttca acagaactac agactggaat cgatgaaggt tttagtgata
     8221 tagaagcaaa tgagtccttt tactctatca aaaacaaact tggtaacaat gcacaagtag
     8281 cgataaaaga aacagtagat attctagaag aagaaacgat ccaaaaagtc gtagagtgtt
     8341 tggaatcggc agagactgtt tttttatatg gggttggcgc ctcttcttta gtagtagaag
     8401 atattctgca aaaatggtct cgtgtcggta agccaatcat ttttgaaaaa gatatccacg
     8461 tcttgctccc gcaacttgtc agcaatgaga aaaagaaagt gctttggctt gtttcaaact
     8521 caggtagaag tgcagatgta gtcgcattag ccgagctggc gaaaagcatg aatatcgaaa
     8581 tcattgcgtt gacgcaattt ggaaataacc cgcttagcaa gatagccgat gtactcgtac
     8641 aaacatctag accaaaagaa atcacgaatc gaagtgcggc aactaattca cttttggccc
     8701 aatttgctac gatcgatatc attttttatt tctatatg
//
LOCUS       DAZUMT010000114         8732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000114 DAZUMT010000000
VERSION     DAZUMT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.116"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..1196)
                     /gene="menA"
                     /locus_tag="ACG8FM_002473"
     CDS             complement(243..1196)
                     /gene="menA"
                     /locus_tag="ACG8FM_002473"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS5095091.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1209..2312)
                     /locus_tag="ACG8FM_002474"
     CDS             complement(1209..2312)
                     /locus_tag="ACG8FM_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS5095092.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2402..3337)
                     /gene="pplA"
                     /locus_tag="ACG8FM_002475"
     CDS             complement(2402..3337)
                     /gene="pplA"
                     /locus_tag="ACG8FM_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS5095093.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3639..5573
                     /locus_tag="ACG8FM_002476"
     CDS             3639..5573
                     /locus_tag="ACG8FM_002476"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5095094.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            6030..6446
                     /locus_tag="ACG8FM_002477"
     CDS             6030..6446
                     /locus_tag="ACG8FM_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS5095095.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6460..7026
                     /locus_tag="ACG8FM_002478"
     CDS             6460..7026
                     /locus_tag="ACG8FM_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS5095096.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            7049..8035
                     /locus_tag="ACG8FM_002479"
     CDS             7049..8035
                     /locus_tag="ACG8FM_002479"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5095097.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8190..>8732)
                     /gene="scm"
                     /locus_tag="ACG8FM_002480"
     CDS             complement(8190..>8732)
                     /gene="scm"
                     /locus_tag="ACG8FM_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5095098.1"
                     /translation="TSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTD
                     TSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDT
                     SSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAG
                     VILLVIGLRITFMSYKKSKR"
BASE COUNT         2568 a         1684 c         1771 g         2709 t
ORIGIN      
        1 gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg aaagccattt
       61 tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc ctgataaacg
      121 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
      181 tttttatttg gctgttctta ttacttgatg cagggcagtt ttttcacaac ctcttcatgc
      241 tgttaaatgg cttgcataat gattgttagt aatagcccca atacataact ggcattgaat
      301 aagaccatat ttttgatgga atagctgaaa ctgataggct ggggtaacgt ttgcttgaat
      361 tgatccaaat gcttcttcac aattggcaag gtcaagaagg tgaccaagat cggccattca
      421 tagataccgc tcaacaaccc cattaaaatc acgaaatagc aagcatacat caatagctgg
      481 aataagactg ttcccgcagg acgtccgata taaaaaacta gtgtatagcg atgattttca
      541 atatcacggt caagatcccg caaattatta gccaacatga tattggcaat cgtaaacacc
      601 aagggtaagg aagctaatac gattgctgct atagcccaaa gatttcctac caaagcaaat
      661 tgtccagatg aaaagttgac tgtcagatca tagacatctg aaccgaatgt attgacataa
      721 acgaccatgg caaaaattcc aaggcccata gtcaccccac tgaaaatttc ccctaaaggc
      781 attcgagata aaggaatcgg tccgaatgta tagaatactc caataaaaca acagataaat
      841 cccatcaaaa gtaaaagcca acccgtattg atcgttagaa taacgccgat aacaaaaaca
      901 aatgcaatca tagctaaaat catgtttcga atcaatacag gagatagatt tctgcggcca
      961 atgacatttt cttcatactt atatacttca gaatgagcct tttcataatc catgtaatta
     1021 ttgatagcgg tagtcgctaa atcaaaaact agcattccaa taaaaaataa aaaagtgttt
     1081 agccaatgta tctcatgaaa aaaagcgata gagaataata cccctaccgc aaaaggaaat
     1141 acactagcca cctttgttcg tatttcaact acttctaaaa agatttttag tgtcatggtt
     1201 cgtactcctt attttagttc agaacggtca cccatcgtgt aatgggagtc ctccgataat
     1261 ttgaaggtgt ctttgattgg atcggtcaca tatactttat cgtcttttgt cacgaagatc
     1321 gcttccgtcc cgttattcaa ttcgctttcg acgtattcca gtcctttttt cacacccatt
     1381 gaaaagactg ctgtagacaa cccgtcacca tcaattgatt tatctgttat gatcgtgaca
     1441 cttgcgatat cattgtcgaa cggataaccc gtttttggat caaacaaatg atggtatttt
     1501 ttgccatcta cttctaaata acgctcgtaa attccagagg taactaatgt tttgttccgt
     1561 tctttaatag aacctaaaac agatccgcga gccatgttcg gatcttggat gccgactgtc
     1621 cagtcttggt tttcgcctct tgggctgtgg cctaagacat agacattacc gcccaagtca
     1681 acgatggcag tggtcactcc ttgttttttc aagactttca ctacttcgtc cgtaatgtaa
     1741 cctttggcga ttgcacctaa atcgatgatc attccttttt cttttagata aacagttttt
     1801 tctttgtcat ttagttcgat cttatgataa tccactaatt tcaatgcctg atcgatttct
     1861 tcttgggaag gttttctggc gtcatcaaaa ccgattcgcc ataattgtgt gattgcacca
     1921 atcgccatat cgaatccgcc ttgagaatct tggctatatt cgtaggcccg cttgactaac
     1981 gtgtagacgt catcagaaac ttttactggt ttgatcccag cttgctcatt cacttcatcg
     2041 atctcagaac ctttttgatt gatcgtgatt ttatcaccaa gttcttttac tcgatcgaag
     2101 gcaggtttca atgctgattc tttgccttca tcatacacac ggatacgaac gtaagtccct
     2161 aacaagaact gttcatcaga atatggatct ttcaatatct tggtagctgc tgaactttca
     2221 gtggttgttt tatttgctga gttgctgcag ccagttagca ccataaaaga gatgcccagt
     2281 gtgattaagg aaaatagtag ttttttccgc atctttttcc tctcttcatt ttgaaaggtg
     2341 agacgactgc ctaatctggc tatcgtccca ccctccaact tttattagta tgattatgtg
     2401 attatttttc tgttacgata ttatctactt cgattgtttg agtgtcgcct ttttcagctg
     2461 cattcactaa ttgttgtgcg tacatgatga atgaatgtga gctgtgtgta gcacctgtaa
     2521 caacttctac attagcaggt gagccatctt cttcgcccat tgctttgacg aattcgtcgt
     2581 tcagagtagg gatgtaagtt tttggttctg taccgctttt tgctttcata ttttcattat
     2641 attctgtatc atcttgtttt gatttaccgt ctttgtcgat ataatcaaag tttgattcag
     2701 ttactttgcc gccagcaact gtcatttcga attgcacacg gtagccgtta gagtagttct
     2761 tttcttcaag cttgtaagtg ccgtctttca aatcagcacc attgtcgatt tcgattgtat
     2821 ctgtgttacc agcttgagca gcttggatca attgttgtgc gtagttttgg aagctttcag
     2881 atgagtgagt agctccagtt acgacttcca caccgcttgc gctttgtgct tttaagaatt
     2941 ggtcattcaa ctcaggaatg tatttttctg ggtttgtgcc agattttgct tccatgtttt
     3001 tgttgtattc tgcatctttt gtttttgatt cgccgttttc attgacgtta tcatattttg
     3061 attcagtgat tttgccgtct tttacaacca tttcaaagac tgcacggtaa ccatttgaat
     3121 aatttttttc ttctaattta tatgtgccgt cttgcaaatc tccgccagct acaacttttg
     3181 cagatgattc tgttgtgctt tttgcagtcg aagattgtgc tgtagatgat tcagaagaag
     3241 agtttgagct tccgttgtca tctgcgccgc aagcgcctaa cactagagct gaaaatgcta
     3301 atacggcaaa tccagttaca aaacgttttt tcttcatttt aataccccac cattccattt
     3361 ctagtccaca atttttcttt gtgattcatt atacattcta atctttttag cctataagtc
     3421 aactattttt gataaataac ccatgtaata tttcaaacgt tttctttcag caaatttcgt
     3481 gaaaaaacgc tttattttaa aatttgaaaa aaatccaaaa ggagttagac aaaagaacag
     3541 aacatctata taatatatgg agattgtgta ataagtatca aactaaaaga attatcatat
     3601 attcattata tagatagtct ataaggagcg gatgatcaat ggcaaagcaa aatattgtcg
     3661 ttgtaggagc aggatatgcc ggtgtttcag ctacaaaata cttggcaaag aaactgaaaa
     3721 aagaagatgt agcaattact ctgatcgatc gtcattcgta ccatactatg atgaccgagt
     3781 tgcatgaagt agctggcgga cgtgtagaac cagaagcaat ccaatacgat ctgcagcatt
     3841 tgtttgctcg tcaaaagaat gtacaacttg tcacagacac tgtaatcggt atcgataaag
     3901 aacgaaaaat cgtcaagacc aaattaggtt cctatccttt tgatcagctg atccttggaa
     3961 tgggcggaga gccgaatgac tttggtacac ctggtgtcaa agcacacgga ttcacccttt
     4021 ggtcatttga agattctgta aggattcggg aacatattga gaagacagta gcaaaagctg
     4081 cattggaacc agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc tgtggttctg
     4141 gatttaccgg tattgaaatg attggggaac tgatcgactg gaaaaatcgc ctagccaaag
     4201 attataaatt agatccaaac gaaatcacac tgatggtcgt agaggcaatg ccgacgatct
     4261 tgaatatgtt ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt aagcacaacg
     4321 tggaattatt attgaatgca ccaatcgtag aagtggctgc tgatcatatc aaattgaaag
     4381 acggacgcaa cattccgact cacacactga tttggacagc tggtgtcaaa ggaacttctg
     4441 acgctgctga ctttggatta gaatcagcac gcggacagcg gttgatcgcc aatgaatata
     4501 tgcaggcaaa aggctacgaa gataaaaaca tctatatcat tggtgattta gtcttttatg
     4561 aagaattccc aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa acaggacaca
     4621 cagccgcagc gaatatcgtg gcttcgatca aaggcggaga aaagcataaa tttaaaggaa
     4681 actatcaagg atttatggtt tctattggtg ccaaatgggg ggtagctaac ctatttgata
     4741 agatccattt aagcggattt ttagctatcg taatgaaaca tatcgtgaat ttgaaatact
     4801 tctttgatat tcgttctggt tattatatgt tccaatattt aatgcatgag tttttccata
     4861 tcaaagatga tcgcaacgtg actcgaggac attcttctcg ttacggcaat gtcttatgga
     4921 gtgtgccact tcgtattttc tatggtttag tttggttcgt tgaagcttcc aaaaaaatca
     4981 ttgggaatgg tgaagtgttc aaaccaagta cttggtttgg tgaaggatca tggttcactg
     5041 gaaacagtgt gttcccttgg acatgggaat acaatgtaac agatgttgcc agtggtgctt
     5101 ctgctgctgg tgatgcaacc acagctgcca gcggagcagg ggcaagtgcc ggagaagcag
     5161 cgactcaagc tgcacatttt ggtttgagtt atgcatacgg tgaagaacca atggctgtga
     5221 ttgagaagtc accagactgg ttcacaaaga tcatgcaagt gatcatgcca aacaatgatg
     5281 tagcaatgtt cttccaaaaa ttcatggtat tctttgaaat tgccttggca ttagcattga
     5341 ttgctggatt attcacatgg ctatgcagtg cgacaacgat tgtattggtg atcacgttct
     5401 gcttatcagg catgttttac tgggtaaaca tttggttcat ttttgttgct ttcgcattga
     5461 tgaacggttc cggacgtgcg attgggttgg atcgctgggt aattccatgg ctacaacgta
     5521 cactaggtaa ttggtggtac ggtagaccaa gttcccagta cggcaagaaa taaatagata
     5581 aagaacgtta tttgaatgca agatttcaat agaaaggggc tgagacaaac cgtcacagcc
     5641 tcctttttat gtaaaattaa aggaataaga tgcccgagat aagcctccat gagcaagaca
     5701 caggtgtcac aacctctgtt acaacctctt gttataaacg gtgttccaga agtagcttct
     5761 tcgaaaaata agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa attccttctt
     5821 atttcttgaa gttgcccact tctgtcacaa cctcttgtta taaacggtgt tccacctgct
     5881 gcttctcggt aataagccca aagaattaga aaacatggaa agctgttttc tattcttcgt
     5941 tcttattact caaagcaaga cacaggtgtc acaacctctt gtttttatgc taaaattggg
     6001 tgagttaatc tggaaaaagg agaacaaaaa tgagccttaa agaatttgct gaaaaaagtc
     6061 tgctcaaacc ttgggatgtt tttattattt tgcttttgac tgttctttct tttttaccag
     6121 tcatgatttt tggtcttcag caaaacaccg caacagcaga aaaagaagcg gtactgcgtg
     6181 tggatggcac agaaatcaaa acattccctt tgatggctgg agaagagagc aacacatata
     6241 cttacaaaga tgaacacgga gattataact tgatcgagat agatggcgat cgagtacgga
     6301 taaaagaagc agattgcgat gaccagatct gcgtacgtcg aggctgggca acgaaaaatg
     6361 gcgaaaccat cgtctgtttg ccacataaat tagtgatcga agtccgttca acagatggag
     6421 gtgacgaaga cagtctgatt tattagacct ttacacacca tgagtaaatt acaaaaaatg
     6481 atttatattt cgctacttgt tgcacaagga gtgatcattg gtttattgga aaatatgatc
     6541 ccttatcctt tttcttttgc gcctggtgca aaactaggct tagccaatct aatcacgatc
     6601 gttgccattt ttaccatgaa aaaacaagac agcttcttac tactttggct acgactattc
     6661 ctgactactt tgctaggcgg aacgatttcc acattccttt atagtatgag tggtgctttg
     6721 cttagttatt ttggaatgtt attggtcaag caattaggac caaaacgtgt cagtatcatc
     6781 ggaattagtg cggccggcgg tttcatgcat aatgtcggac agcttttgat ggcttctttc
     6841 attgcacaat catggacagt catgttgtac ttgccagtcc tttcagtttt agggatttta
     6901 tcagggattg caatcgggat cgcagcgaat tatcttttac gacatgtgca gacacttcaa
     6961 cgattccaaa aagattatga gcagggaacc aaacaaaaat ggagcccaat gtttaccaaa
     7021 aaataatcgt cacacaagga gtttttttat gaagctacat ccaatgtggg aaaactatcc
     7081 tgctttaagt aaagaattga cagaaacact aaaacggatg gaagaaactg tccgcctgaa
     7141 aaacaaacct gtcgaacaag caatcaaaga aacgatccat gccggcggaa agctgttgcg
     7201 tcctgcctat cagctattgt tctcacaatt tggaccagag caagatcgag acaaagccat
     7261 cgctttggca gcttctatcg aaatgctcca tacggccaca ctgatccatg atgatatcgt
     7321 tgatgaagcg gatctgcgac gtggtcagcc taatatccgt agtcagtttg gtaatgtcgt
     7381 ttctgtctat gcaggagact atttatttgt ctgctgtttt aaactattgt ctgattactc
     7441 cacttccttg aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat tgaatggcga
     7501 attagggcag atggatgatc gctataatta tgaactttct gtaaaagaat atttggaaaa
     7561 tatttcaggt aagacagctg aactgttcca attaagttgt tctgttggcg cttttgaaag
     7621 tggaacaagc gagcgcttcg caaaaaaagc aggagatatc ggactttcta tcgggatggc
     7681 ctttcagatt atcgatgata ttttagatta tacaaaagag agccaggaaa tcggcaaacc
     7741 tgtactagaa gacatgcgtc aaggggtcta ttctttaccg ttgatctact ctttgcaaaa
     7801 aaacaaacca aagctactac cttatttaga gaagaaagcg gcaatgacag aagaagatgt
     7861 cgacgccgta cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta ggaagttagc
     7921 ggcctcttac acagaaaagg ctttgaagga aataaagaaa ttacctgcca cctctcttcg
     7981 aacaaaagag aatctctttt cccttaccca actgattttg gatcgcaaag actgaatagg
     8041 gaaaggttcg atacgtaaat aaatttctca gaaggaaatc tacgtgtttt ttgacaaatt
     8101 gaattgtagt tcttgtattt atgataaaaa gcaaaaataa agagcaacgt ccgttagcga
     8161 cgttgctctt ttttatcttg aatatttttt taccttttcg atttcttata actcataaaa
     8221 gtgattctta aaccgattac aagtaatata acgcctgcga gtgttatcca attattcgat
     8281 tgacttccag ttttaggaag tgctcttttt gatttgtttc cattgtcatt gcttgtttta
     8341 ttagttaagt taaagttcgt cctgttatcc gaacttgttt ttgagaagga gacacttgca
     8401 tgacgtgtat cgcttgtggt agacgacgtg tctgtactat cgcttgaagt tgatgaatct
     8461 gagctatcac ttgtggtaga ggatgtatct gtactgtcac ttgaagctga cgtgtccgtg
     8521 ctatcacttg tagtggtaga tgaatctgag ctatcacttg tggtagaggt agtatctgta
     8581 ctgtcacttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     8641 tcacttgaag ctgatgtgtc cgtgctatca cttgtggtag aggtagtatc tgtactgtcg
     8701 cttgaagctg acgtgtccgt gctgtcactt gt
//
LOCUS       DAZUMT010000115         8675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000115 DAZUMT010000000
VERSION     DAZUMT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.117"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACG8FM_002481"
     CDS             <1..1101
                     /locus_tag="ACG8FM_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5095099.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="ACG8FM_002482"
     CDS             complement(1280..1609)
                     /locus_tag="ACG8FM_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5095100.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACG8FM_002483"
     CDS             complement(1599..1862)
                     /locus_tag="ACG8FM_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5095101.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACG8FM_002484"
     CDS             complement(2322..3125)
                     /locus_tag="ACG8FM_002484"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5095102.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACG8FM_002485"
     CDS             complement(3103..4227)
                     /locus_tag="ACG8FM_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095103.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACG8FM_002486"
     CDS             complement(4243..5058)
                     /locus_tag="ACG8FM_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5095104.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACG8FM_002487"
     CDS             complement(5048..5920)
                     /locus_tag="ACG8FM_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5095105.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACG8FM_002488"
     CDS             complement(6005..7294)
                     /locus_tag="ACG8FM_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5095106.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACG8FM_002489"
     CDS             complement(7438..7950)
                     /locus_tag="ACG8FM_002489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095107.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACG8FM_002490"
     CDS             complement(8017..8160)
                     /locus_tag="ACG8FM_002490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5095108.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAZUMT010000116         8493 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000116 DAZUMT010000000
VERSION     DAZUMT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.119"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8FM_002491"
     CDS             complement(<1..149)
                     /locus_tag="ACG8FM_002491"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5095109.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8FM_002492"
     CDS             complement(593..1714)
                     /locus_tag="ACG8FM_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS5095110.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8FM_002493"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8FM_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS5095111.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8FM_002494"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8FM_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS5095112.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8FM_002495"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8FM_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5095113.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8FM_002496"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8FM_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5095114.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGEGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSIPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8FM_002497"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8FM_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5095115.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8FM_002498"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8FM_002498"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095116.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1728 c         1485 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggaattga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccctcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DAZUMT010000117         8364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000117 DAZUMT010000000
VERSION     DAZUMT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.120"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(449..946)
                     /locus_tag="ACG8FM_002499"
     CDS             complement(449..946)
                     /locus_tag="ACG8FM_002499"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS5095117.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(986..1795)
                     /locus_tag="ACG8FM_002500"
     CDS             complement(986..1795)
                     /locus_tag="ACG8FM_002500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5095118.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2129..2986
                     /locus_tag="ACG8FM_002501"
     CDS             2129..2986
                     /locus_tag="ACG8FM_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5095119.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2979..3881
                     /locus_tag="ACG8FM_002502"
     CDS             2979..3881
                     /locus_tag="ACG8FM_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5095120.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3914..4264
                     /locus_tag="ACG8FM_002503"
     CDS             3914..4264
                     /locus_tag="ACG8FM_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5095121.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4313..5236
                     /locus_tag="ACG8FM_002504"
     CDS             4313..5236
                     /locus_tag="ACG8FM_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5095122.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5314..6207
                     /locus_tag="ACG8FM_002505"
     CDS             5314..6207
                     /locus_tag="ACG8FM_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5095123.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6245..6808
                     /gene="amaP"
                     /locus_tag="ACG8FM_002506"
     CDS             6245..6808
                     /gene="amaP"
                     /locus_tag="ACG8FM_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5095124.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6822..7010
                     /locus_tag="ACG8FM_002507"
     CDS             6822..7010
                     /locus_tag="ACG8FM_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5095125.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7023..7925
                     /locus_tag="ACG8FM_002508"
     CDS             7023..7925
                     /locus_tag="ACG8FM_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5095126.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7957..8199
                     /locus_tag="ACG8FM_002509"
     CDS             7957..8199
                     /locus_tag="ACG8FM_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5095127.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         2891 a         1463 c         1642 g         2368 t
ORIGIN      
        1 ctcagttggt tagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaagg
       61 tacataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
      121 cagcttctta tgtttttact tggctgttct tattactgca gatgcaagct tttgaatacc
      181 gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag tattagctta acaaacggaa
      241 aaatagttcc tcatattttg ccgtttgtta agctaatacc gagggtcagc tgattgaaca
      301 ccatttaaga aagaggtcgt gagcctgccg tttcactctg tgtcataaaa aagccaatct
      361 actttgatag aaaaagcacg taggactaaa acagttctgc gtgctttttt tattgtgtgt
      421 taaatatcgt aaatgaagat agatattttt atgattcgac gatagagacg ttgaaatctt
      481 tcaacagctc taaagcggat tctccgtgtt ttctactgga gactaacatt ggatcaacaa
      541 aaaagtctgc ttcaggaaaa gcactctgca ggcctatcag attagagagt acacacaagt
      601 ttgtttcaat cccgactact tcgatctctt tttgttcttc tttgctttca aataaagttt
      661 cctggatttc agctaaagtt tcaggtggaa tcgtaaagta atacttttta atcactcgtt
      721 cattttcttg tggagaaaga agagggatca attgcaaatc ttctcgttct tcttctttgt
      781 ccttcagttc aactggtata tcccgtgtga agagaatata ttcattattt tggcgtgctt
      841 tagctaatct ttgagcaata cgttctgcca attgatctga ctctggaata taaaattcgc
      901 tgtttggatc aagaatatga ttttgcatat caataataac taacattttc tttcctccta
      961 cattcatatc gtcattttat tctttttaat agaagttttt taaaatataa gcaacaccag
     1021 cttcttcatt cgtcagtgtt acttggtttg caatttcttt tacttgtcgc tgtgcattcc
     1081 ccatcgcaat actcaacccg ctgtaattca acatagcaat gtcgttttct tgatcgccaa
     1141 aagccatgat gtcactcgat tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag
     1201 ctttagtgat acctgtaggc gtgatttcta agaaatgcgg atcagagaat actgattgga
     1261 taccatctgc ttgcagatct ctttgtagct tctgtagttt ttcttcactc ttatctgtta
     1321 atgctaattt aagatagcta agagaaggat cttccaacat atttaaaatt tgatcaaaag
     1381 aatggttctg taaattgaaa tgttcataat aagggtcatc tttgactaga tttgattcat
     1441 cgaatgtcag ctgatcatct tgggtttctc ggctagcaat gatggatatt ttcgcttctt
     1501 gagcatagaa gaaggctttt ctcagtgtat cgctatctat ttttgaacgt ctgagaactt
     1561 tcgccatttt tccatccttc atttcagcaa tcagtgaacc attggaagaa atcaaatagc
     1621 caccgattcc taattcggaa aaaatatctt tagcagaaat gctgttcctt ccggtagcga
     1681 cggttacgat tccgccattt tcatcaaatt tccgaagtgc ttggatattt tcttctggta
     1741 tctttccatt tcgatcaagc aaggtaccgt ctaaatcaat cgctgctagt ttcattttat
     1801 cttcctactt tctttttaca tatctatttt cctgttacct taagattaaa tgagaattta
     1861 aaaaaatgaa actaacttgc tttgtcatta tgaatttttt caaaaaacat tcttaagaaa
     1921 aaggtggcta tatatagtag tttttggtca atacctttga ataaaaagcc atgaaaaacg
     1981 aatagttagc gcttaccgat gcgttttgtg ataaaaaaat tttaaaatat tcaatgaaaa
     2041 caaagcatat attttgcttt aaaaccaaat gaggaatatt ctagatgtag aacaggttag
     2101 aaaaagatta gaaaaaggag tgattttgat ggagaagaga tacggcgtag ttcctgtggt
     2161 aactgcttca gacgaaaatt atgctcctta cttgagcgtg atgatcgcaa ctgcattaga
     2221 aaattgcaat aaaacaagac gcataaaatt ttatgtgatt gacgatggtc tatcagaata
     2281 tagtaaagaa ggattggaag aaacggtaaa taaatattca agtaatgcaa gtatccaatt
     2341 cctgacagtg gaaaaagata tctacgaaga cttcctagtc agtgaccata tcacaactac
     2401 cgcttactta agaatttctt taccaaactt attagctaaa gaggattata agaaagtcct
     2461 ttacttagat tcagatgtgt tagtactaga cgatatcgta aaactatatg atgagccgtt
     2521 aaatggaaaa acaatcggcg ctattatcga tccaggccaa gtgaaagctt tggaaagatt
     2581 aggaatcgat tccgatgact tgtacttcaa ttcaggagtg atggtgattg acattgatca
     2641 atggaataaa aaagaaatta cagaaaaaac tatccattac ctcagtgaaa atggcgatcg
     2701 aatcatctat cacgaccaag atgctttgaa tgctgtactt tatgaagatt gggaacaact
     2761 tcatccaaaa tggaacatgc agacttcgct gatttttgaa cgtcatccag caccaaatga
     2821 aaaatatgaa cgtcagtata aagaaggaaa tgaaaaacct tcgatcgttc actttacagg
     2881 acatgataaa ccttggaata cgttgaaaga tcatccgtat accaatttat acttaaaaaa
     2941 attagcacac agtactctaa cgaaagtagg cgagatcaat gaataaaaaa gaaatagcag
     3001 tagtcgcaag ttgtaacaca aaattcgtac cacatttagc tgcattattt gtctcagtat
     3061 tagataattg caacccgtct aaatttgttc ggttctatgt gattgacgat gatatcgatt
     3121 ttgaaagtaa acagctttta cgcttctcag tgaaaaacgc acgaatgaac tcagatgtgg
     3181 aattcttgaa aatcaataaa gaattcttta caaatgtggt aatcagcgat cgtattccag
     3241 aaacagctta ttatcgtatt gcgattccgg aattgttccg cggcacagaa gttgaacgta
     3301 tcttatatat ggattgcgac atgatcgctt tacaagatat ctctaaatta tggcgtcttg
     3361 acttcggtga ttcaatcgta gcagctgttg aagacgctgg gttccatcaa cgactggaaa
     3421 aaatgaaaat ttcagctaaa tcaatgcgtt atttcaactc aggtttgatg ctgatcaatg
     3481 tgaaaaaatg gttagatgaa aatattacgc aaaaagtatt agatttcatc gaacataatc
     3541 cagaaaaatt acgattccat gaccaagatg cattaaatgc tatcttacat gaccgttggt
     3601 taccacttca tccgcgttgg aatgcacaag gctatatcat ggcaaaagca aaaaaacatc
     3661 caacagcagc aggtgaacgt gaatacgaag aaactcgcaa caatccttac attatccatt
     3721 tttcaggaca tgtgaaaccg tggagcaagg attttgaagg gccaacgaag aaatattacg
     3781 aaaaatatgc agggatgacg gctttccgtt gtgtagctaa attccctaaa tatccaaaat
     3841 atgcaaaagt tcaaccaaaa acagatgtag ttgttaaata atagaagaca acaactgaag
     3901 agggggagta ggaatggaaa ttacaatgtc taaaaatgca gtagagacgt taattgaaaa
     3961 ggttggggaa aatacaaaaa tcgcactcgc gcttatcaat gattccgatc catttctaag
     4021 ggacaaaggt gcttttgcaa aaggcagctt ttttcaaatc attccttttg tttcggaatt
     4081 tggagaatat gcgactaaaa tcgagcatcc attattggac atctatacat ctaaattaga
     4141 gcaaaattat ttcggtaaac gattgaacat ggacttcaac aaacagcttg acagcttttc
     4201 attagaaaat gaaatcgcag tactcgatta taatatcaaa ttaaagaact gcttcttcag
     4261 ctaatagttt cgagaaagtc caaaaaggag gtatacattg agatactact ttgtggatga
     4321 agaaaagaat gtaatagaaa cagatgaaaa aaaatataat tggctcgtac ttgatagtgc
     4381 aaagcccgaa gagattgagc aagcaatcga agaattcgag cttcctgatg atattttcgt
     4441 aggaaccgaa tatccggaag aagtttctcg gatggagcgc ctttatgaga cgaaactaga
     4501 gcatccattt tctttggtag tgattaatct agatcagtca gataatcgta tcgaaaaaaa
     4561 attaacgcca atatcttttg tactttccga taatctcttg attacttgta tcgatagaaa
     4621 aaccgaattt gtggatcggc tgctgacaaa gcatggaaat catttaaact cgtttgaaaa
     4681 gatcatcacg tatacattat tagatattta cacccattac gtgaaagaac taagagagat
     4741 gaaaaagcga attgacgctt tggataaagc agctcgtaaa acaacggaaa atgaagaact
     4801 gtataaacag gcagatcttg aaagagacat cgtatatatc gatcatacct taagagatca
     4861 acgggaaaca atggatcaat tatggaattc tgaaactttc atcaaacgtt tagacgatga
     4921 acagctactc tatgatgtcc atttgagaca gcgacaaaca gaaaaaatga tcgagatcta
     4981 tcgagattta ttagagagta ttggtgatct attcaacggg atgatggaca ataatctaaa
     5041 tcatatcatg aaatatctag attcagctgc attaatcatt tccgttccgg cgttgttcgc
     5101 agggatttgg ggaatgaata caggaggatt accgggtaaa agttctgctc taggctttat
     5161 actcgtagtc tttggctctt tgatagcagc agtagtttta ggttaccatt tataccggaa
     5221 agattataca aattaaagaa tctatttaaa acaggtatca aacaaagaaa tttgttttgg
     5281 caatacaatt taaaaactag gaggaataca gcaatgacag aaccaaaatt aatcgacccg
     5341 cgaaacttgt accatacgga gaaatttcct aaacaagatc aggatacacc agctttacag
     5401 cagaatatga ttcctgttcc tgattgtgga gaagaatcct atgaaggcaa taatcaacta
     5461 gaaaaccgcc gtgtactaat cactggtgga gattcaggga tcggtcgtgc agcagcgatt
     5521 gcatttgcta gagaaggggc agatatcgct cttcaattct tcccaggaga agaaaaagat
     5581 gcagaggaag tagcaaaata cgtgaaagtt gcaggacgta agattgtctt actacctttt
     5641 gatcttcgtg acgaacaagc acctaaagaa atcatagata aagccgtgag tgaacttggc
     5701 gggctagata cgatggtact aaacgcagga cagcaaattt cttgtcagtc tattgctgag
     5761 ttaccacttg agcaagtcaa agacacattt atgataaaca tcatcgctat gtttgcttta
     5821 gtgaaagaag ctgttccgca tatcccagca ggaggagcaa tcgtaacaac aacttctgtt
     5881 caagcattca atccaagcga acatttatta gattacgcag caacaaaagc ttcaatcgct
     5941 aactttacga tcggtttagc aaaacagctt gcacctaaag gaatccgtgt aaatggtgta
     6001 gcacctggtc caatctggac accattacaa ctagacgaag gacaaccttc tgattcgatc
     6061 cctgaatttg gtcagcaaag tttactagaa cgtgcaggtc agccaggaga attagcacca
     6121 gtttatgttt tcttagcatc gaataaagca agttatgtta cagcccaaat ttacggagta
     6181 actggtggag aagcgatcaa cttataagaa gttgatcgtt tcacccctat gaggaggtgc
     6241 gaaaatgaat aaaggtgtaa aaacgatcgg agtgatcgga acattacttt tgctatctgt
     6301 gcttttgttt gcgatactat taagtgcaac ctatccattg ccttttcagc tagagaagtt
     6361 tcgattcttt tccatcacta attcatacgt gcaacaatat gtattttggc ttgccgttgc
     6421 atttgctatt ttgacaatta tcacgttact tgttttcctg ttttatccaa aaacaatcgg
     6481 aaactttcta ctgaaacatg gtgatgggga gttgacgttg gataaaaaag caattgaagg
     6541 aatggttcgt tctcacttgc atgaagatga attcatccat tcaccaaaag taaatgtcaa
     6601 agcaacgaaa aacaagatcg ccgtcaatgt aaaaggggag ttgaaacgaa cttcagcact
     6661 cattggtaaa acaggaacat tgatgaaaga aatcgaaaat gaagtacaac acatattggg
     6721 aacaaaagag ccagtgaaag tatcggtgaa atacacaggc tttgaagaac cagctaaacg
     6781 aagcaatgac cgcaaacgag tagaataggg ggggaccaaa aatgaaagaa ctattttcga
     6841 catacaagct ccctatcatc tttggggtac ttggattgat attagccgtt ttatggatca
     6901 ccgtgggctt tttcaagacc ttattgatct tgatcatggc agcgatcggc gtcgctatcg
     6961 gtttttatct gaaacaaacg aaaatcttag atcactattt taaatcttag gaggaaacat
     7021 ccatgaataa caataacaaa aataataaca ataaaatgaa caaccaaaaa ccagtgtctg
     7081 cacatccaga cccaacgaca ccaaaccaat caaatggtgt gaacgatgaa aagaacaaaa
     7141 cagcaccagt atctgcacat ccagatccag tagtccctaa cgttcaacaa ggagtagaag
     7201 agaagaaaca tgatgcagaa aagcatgcac ctcatcatcc aggaactcca gaatctaaag
     7261 ttccagaaca acatgatgca atcgatcctt ctaaacctgt ggaagagcaa aaacatcctt
     7321 ctgaccatgc gaaagcacaa gaaaaactta gttctgacca cacaaacgga caaggaaaac
     7381 ttggttctga tcatacaaat aaccaaggaa agcatgattc tgaacaggaa gattcaatgc
     7441 gcggtgagct gaattatgaa gataaagttg tgcaaaaaat catcggaatc gcaattgaac
     7501 aagtagacgg tttattatct gcaaacggtg gtttcttctc taatgtggca ggaaaacttg
     7561 tgaatacaga taatgtaacg gctgggatcg aaacagaagt cggtaaaaaa caagtagcag
     7621 ttgatatgga tgtcattgtg gaatatggca aagatatcga aaaaatcttt gaagaaatgc
     7681 aagaagtaat cggcaaagaa gtaaaaaata tgacacattt agaagtgatc gaagtcaacg
     7741 ctaacgtagt ggacatcaaa acaaaagaag aatttgaaaa agatcaagaa acggtgcaag
     7801 acaaagtaac tgacgcagcg aaaaaaaccg gtgaatttgc ttcgaatcaa acagataaag
     7861 taaaatcagc agctggaagc ggcgcacaaa aagtaaaaga aaatacagaa ccacgggttc
     7921 agtaataagg aagtaaaaca ttaggaggaa cgaaacatgg gatttatttg gtcacttatc
     7981 gtcggaggaa ttatcggagc aattgcagga gcaatcacga ataaaggttc ttcgatgggg
     8041 attattgcaa atgtcgtagc aggtttagtt ggatcagcac ttggtcaagc attattaggt
     8101 tcttggggac caagtattgc aggaatggca atcgtgccat cattgattgg tgccatcatt
     8161 ttagtactaa tcgtatcatt ctttgctcga aaactgtaaa aagactttta aaaagaggtc
     8221 gtgaaaaagc tgttcttcat caagtaataa gaacagccaa acaaaaatag cttctcatat
     8281 ttttcatttg gttgggcata ttatcgaaga tgcaagcttt tgaacactgt ttatacggaa
     8341 taagaggctg tgacaagact tttg
//
LOCUS       DAZUMT010000118         8312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000118 DAZUMT010000000
VERSION     DAZUMT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.121"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8FM_002510"
     CDS             complement(105..629)
                     /locus_tag="ACG8FM_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS5095128.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8FM_002511"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8FM_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS5095129.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8FM_002512"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8FM_002512"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS5095130.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8FM_002513"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8FM_002513"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5095131.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8FM_002514"
     CDS             3157..4026
                     /locus_tag="ACG8FM_002514"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5095132.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8FM_002515"
     CDS             4052..4567
                     /locus_tag="ACG8FM_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS5095133.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8FM_002516"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8FM_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5095134.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8FM_002517"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8FM_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095135.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8FM_002518"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8FM_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095136.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8FM_002519"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8FM_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5095137.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8FM_002520"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8FM_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS5095138.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..>8312)
                     /locus_tag="ACG8FM_002521"
     CDS             complement(7873..>8312)
                     /locus_tag="ACG8FM_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095139.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         2374 a         1714 c         1500 g         2724 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt ga
//
LOCUS       DAZUMT010000119         8249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000119 DAZUMT010000000
VERSION     DAZUMT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.122"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACG8FM_002522"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACG8FM_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5095140.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACG8FM_002523"
     CDS             complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACG8FM_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS5095141.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACG8FM_002524"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACG8FM_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS5095142.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="ACG8FM_002525"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="ACG8FM_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS5095143.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACG8FM_002526"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACG8FM_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS5095144.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACG8FM_002527"
     CDS             complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACG8FM_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS5095145.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8134..>8249)
                     /locus_tag="ACG8FM_002528"
     CDS             complement(8134..>8249)
                     /locus_tag="ACG8FM_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFS5095146.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
BASE COUNT         2200 a         1816 c         1440 g         2793 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc aaaaaaactt gcggcagctg tcagtagctg ctgcaagttt tcgttgttat
     7441 ttaagtaagg ctgatcagtc tgtttcaatc agtccgtatt ttccatcttt acggcggtaa
     7501 acgatacttg taccatttgt ttctgcatct tcaaaaataa agaagttatg tcccagcatg
     7561 ttcatttgta agactgcttc ttcactatcc attggtttca atgaaaggcg tttcgtacgt
     7621 acgatatcta attcagaatc attttcgttt tcgtcctctt cattgaataa agcagcagca
     7681 tctgcagtat tcatgcccgt ttcacgggat ttacgattaa tttttgtttt gaatttgcgg
     7741 atttggcgtt ccaatttgtc aacaaccaaa tcgatacttg cgtataaatc tggagaagtt
     7801 tcttcggcac gtaatacaag ataaggtaat gggatcgtta cttccacttt tgctgttttt
     7861 tctgtataaa cttttaagtt cacatgcgcc gttgcatcgg gtgcgtcact gaaatagcgt
     7921 tctagtttgc ctactttttt ctcaacgtag tcgcgaatcg cttcagttac ttcgatattt
     7981 tcgccgcgta cattatatct aaacataaaa attacccctt tcgtctacca caatcaagaa
     8041 ggagaaggac cactctttcc ctcttcttaa ttctattata tcgaactcta ggttatttgc
     8101 aaattaaatc gctatcaaat ttccacatct gattcatctt gccaaagaaa tggttcggat
     8161 gattttcggc tgtgcgttct gcaagcaatc tgctgcatga aaaagcgtct gtcctgttgt
     8221 atagacatcg tcaataatca atatttttt
//
LOCUS       DAZUMT010000120         8191 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000120 DAZUMT010000000
VERSION     DAZUMT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.123"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8FM_002529"
     CDS             476..1339
                     /locus_tag="ACG8FM_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5095147.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8FM_002530"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8FM_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5095148.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8FM_002531"
     CDS             3339..3860
                     /locus_tag="ACG8FM_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5095149.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8FM_002532"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8FM_002532"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS5095150.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8FM_002533"
     CDS             5941..6759
                     /locus_tag="ACG8FM_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS5095151.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8FM_002534"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8FM_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095152.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8FM_002535"
     CDS             7228..7806
                     /locus_tag="ACG8FM_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5095153.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8FM_002536"
     CDS             7901..8038
                     /locus_tag="ACG8FM_002536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095154.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2451 a         1479 c         1615 g         2646 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacat cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac t
//
LOCUS       DAZUMT010000121         7943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000121 DAZUMT010000000
VERSION     DAZUMT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.125"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2672)
                     /locus_tag="ACG8FM_002537"
     CDS             complement(<1..2672)
                     /locus_tag="ACG8FM_002537"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5095155.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2659..3021)
                     /locus_tag="ACG8FM_002538"
     CDS             complement(2659..3021)
                     /locus_tag="ACG8FM_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095156.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            3240..4460
                     /gene="sstT"
                     /locus_tag="ACG8FM_002539"
     CDS             3240..4460
                     /gene="sstT"
                     /locus_tag="ACG8FM_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS5095157.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4607..5242
                     /locus_tag="ACG8FM_002540"
     CDS             4607..5242
                     /locus_tag="ACG8FM_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5095158.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5433..6173
                     /gene="truA"
                     /locus_tag="ACG8FM_002541"
     CDS             5433..6173
                     /gene="truA"
                     /locus_tag="ACG8FM_002541"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5095159.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6318..>7943)
                     /locus_tag="ACG8FM_002542"
     CDS             complement(6318..>7943)
                     /locus_tag="ACG8FM_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095160.1"
                     /translation="KNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLSRIIG
                     LDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLT
                     MVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCY
                     EIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLK
                     ATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLF
                     LFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNG
                     FSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAP
                     EDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSN
                     NKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIA
                     SRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2626 a         1234 c         1491 g         2592 t
ORIGIN      
        1 attttttgtg tcgcccatgt tgcactgtac tggttacctg caacaagatc atattgtatc
       61 agacgataat cttctagaat ttctttttgt ttctcagtta aatttttttc ttggataatt
      121 tgactatcct ctgtaacaaa gacctgtttt ccattaaaac gattagacaa gctttctgta
      181 ggatcaattg ttaatgcagg tagatgatta gatacttctg tcaacaacgc atagtaagga
      241 gttactttga tattcgcatg ctcaagagcc atagcaccaa agttattagg agcaacaact
      301 tctttattta acgtagctgc ttgttcacga ctatacttgt tgttataaat gaaataatct
      361 gtctggtgct gttctaatcc atattttttc gaatcattcc cactatagat tgcgggtaaa
      421 tggtcaccat aaaaaacaaa ggtaatcggt ttatcgattt tgtcgatttc ttggatgaat
      481 ttttttactg ctgtatcggt atagtgaagg ccttgcatat aagtttgtaa ttcttttctt
      541 ctattaggaa tgacagcagt tccttcagca gtgtagttca attcactata gtaatcatca
      601 taaggcatat ggttttgcat agtggataat tggatgaatt gtgacgtttc tgtattgctt
      661 tgtaaagctt tcaaggtttc tttgtatgct gaatcatccg atatgcgtgg gctttgttct
      721 attttatccg tataagttaa tttatccgga ctgtctaaat aatagaacct gtcaaaacca
      781 aatttatcaa acacttgttt tctgcgataa agagcagctg taaaaggatg gatggctatt
      841 ttttcatcga atagatcggt aatatttgga cttattttct gacgctccac tagctgggta
      901 taaggtgtaa ctaacgaagc agacaggtta ctgatactca agcctgtcag tccttcccat
      961 tccatgttag ctgttccgcc accataaccg acggataaca tcaatcctcc tgttccttct
     1021 gttataatgc ttttgatgta aggaataggg tctgtaggta ctgtcagatt agggacacgt
     1081 gcaggatcac tgaagctttc acttaaattg agaacgatcg tctgattgtt taaccagtca
     1141 cttcttgttt ggttgatttc atctgccttt gtgttgtact ttttcattat tccattaatt
     1201 ttttctttcg aatagccagc aggttcttcc atgattttaa tgtcgatatt gtttaggaat
     1261 tgaatgactg gtccgttatc ttttacggcg ccttgctggt taaagaaagt cttgtttact
     1321 ttaaataaat taaacagcaa gaaagaaggc gaatttttgt gattaatgaa aaagacaccc
     1381 gaaaaacatc ctaataatac agcgatactg ataaatcgac gtttccaatg aatcttcaag
     1441 gaatactgtt tttgtagtct acgttgtaca ataatactag taatcaataa taggagaagg
     1501 atcaatccgc ccacgattaa taagactggg ctaaccatcg ataatagttc tgacaatccg
     1561 gtcagtattt ttaaatctga aggaaagaca ggctcttccc gcatttccat tttgattact
     1621 gtcgaaatag tgagcagtaa gttaatgatc aaggtagcag tcaaggcgta ccaaaaacga
     1681 ttggaaatca acaatagtaa gaagaaaaat cccataataa tgatgattgt caggatcatg
     1741 ggtgcttgtc gctgagtaaa tatttttaag gcaacctgga tagtttcttt ccaaccttct
     1801 tttggttcta aaacgaaaat ctgcacgaag gtaaagcagt aaaaaaatgc tgcaactaaa
     1861 aaataacttt tatgaacaaa aagccaatga attctttttt tccaacgctg agttaattgt
     1921 tcaatcgaat caaaagcgaa gtattcgatg aaacgatgaa tgaaacggat tttttgtata
     1981 taaaataaaa agataactag gagggacact gctaataaat agcaaataaa aaactgcccg
     2041 atatttatca gccatgcctt ccctgagttt ggaaattcac gtttcataaa tgtagatacg
     2101 cgataagaaa ctaaagggaa attacatata acttgagtga caatcaaaaa tacagcaaga
     2161 aaatttcctc ttccttttat acctaattta tcagatagca tttctaaaaa taaaaacgaa
     2221 gagaaagtat aatacattgc gaagagagaa tatggtttag aaaacctcag cgctgtatct
     2281 gtatttcctc tgatgatgag tgaaatttgt gtcatcaaaa taatcaaacc gaataaaaca
     2341 atcaagctga taaatacttg aggaagctta tagcggacct tttctttcca gttccattct
     2401 ttcaaaccat aacccattaa aaataaataa aaaatccata aaatactctg tccattctgt
     2461 aatgcaaacg ggtctttatt aaatagagta gagcagccaa ctaatccaat tgttaaaaat
     2521 aatagtagat atttaattgt tgttttcgat aatttattta aaaatccaac tgtataagga
     2581 atactacaaa aaagcaaagt acaagaaaca gcataagtaa agtaattctg actgataggt
     2641 gttaggataa gccaataatc acgaatattc aagctgccag cttgtacgta aaaaaaagat
     2701 attgcaaact agtgtggcta ttaggaatat aaaggcaaaa cgacttaaat aatgtaaaac
     2761 ttttaaacgg gcgatttttt gatattggta acccatgtaa agtgggatca aatttgcacc
     2821 aataacaacg gaagtttgta aaaatgtata gatgtagaat cgaccagggt ttgttgcata
     2881 tgtaggaata gtgactgctg aaatcaatat acgctgtaaa accatcatta tacaagcgac
     2941 aaagattgtc aggaagccat aactctcctt ctttattttt ttcaagaata aacacctcac
     3001 aaaaaaattg gacaaaaaca taagaaaacg tttcctgata agttgcccat taataacatc
     3061 ctaacttaga atatctcaaa aaacaacgtg tagacagaaa aagtacaaaa ttagcaagaa
     3121 atggttgttt gtattgttag aatttttaga aaattatacc tttagtttct ttgcataaaa
     3181 tgatacaatg attgttaaaa ttgtgaaagg gtggtcattt gttagagaag tttttggata
     3241 tgtcgttagt tcaacgtatt gttgttggta ttttagtcgg aatattgttt ggtatctttt
     3301 tccccacatg gacatttatt tcaatattgg gaacgttgtt tgtcagttgc ttgaaggcaa
     3361 tcgcacctat tctggttttt ttcttgacga tggcgtcaat tgccaagcat aaaattggga
     3421 ataagacatt cgtgaagcct attttgatct tatatttagt gggaacattg ctttctgcac
     3481 ttgtagcagt tattgtaagt tcaattttta ctgttcctat tactttacaa gaaacagtat
     3541 ctgaaaaagc accgcaaagt ttggaaagtg ttttatctac tatgttgacg aatgtcacac
     3601 aaaatcctgt tcaatctctt attgatgcga attaccttgg cgtcttattc tgggctgtat
     3661 tgttgggact agcttttcga gcacgttcag aatcgacaaa agagttggtg gatcagatat
     3721 ctatagcgtt gtcacaagtc gtacaagtga ttattgcatt tgctccgata gggattttag
     3781 gattggttta tcaatcaatt gctacgactg gaatagctgg attggcagag tatctgcagc
     3841 ttttacttgt tttgatcggt acgatgctgt ttgtcgcatt agttgtgtac ccattcctca
     3901 cgtttttgtt tattaaagaa aatccctatc cattgatttt cttttgtttg aaagaaagcg
     3961 cacttcccgc tttctttacc cgaagttctg ccgcaaatat cccgataaac atgatgttgg
     4021 ctgaaagatt gaaattgaca aaagagtctt attccatatc gatcccgtta ggtgctacga
     4081 tcaatatggg cggtgccgcg attaccattt ccttgatgac gttgactgct gtacattctt
     4141 tagggattga agcacctttt gttttgaaag tccttttatg tattttgtct gctttagctg
     4201 cttgtggagc ttcaggtgtt gcgggcggtt cactgttatt gattccatta gcatgtagtt
     4261 tgtttggtat ctctaatgat gtcgcgatgc aaatcgtcgg tattggattt attatcggtg
     4321 ttatccagga ttcaatggaa actgccttaa attcgtcaag tgatctgttg tttactgcaa
     4381 taggagagct aggcgcgcgt aaaagaagtg gggaaaaaat caaattgaaa gattgtttag
     4441 atggattaag tgaacgttga acgttagata tttagtattt ttaaaatgat tcgagttttt
     4501 aatgagttat atagatgttc gtgtaaatag aggtttctgt taaatcaaga taaagtcgct
     4561 tataataaca attaaaagca taggtaattg ttattggagg cgtatcatgg attttttacg
     4621 taaatttggt tttttctttt tattattagg cgtgctgagt gattttttga ctccgtacgt
     4681 attaggattg tattatcctg aattagatca aatgaagagc gtgatcagtg tatttggaga
     4741 ggtttccagt cctgtaagaa aagcgtttct tatttggtct gtggtttcag ggatcttttt
     4801 catcctgtca ttgcctgcat tgtatgctgc atttgtaagt acatctcgtg tgttagctac
     4861 cctagttaca gcagccatcg cgttctatgg tataggtgat tgtatcttta caggtttgtt
     4921 cagtatcaat acccatgaat cttcgtggaa tgtctcgaca tgggtccata atatcggatc
     4981 aggtttaggt tacagtggtt ttttattgtt tccagttctt cttttcttga tctatcgaaa
     5041 aaatgggcaa atggacgtaa gccacttata ccttggactg tctgtgatca gtttgctttt
     5101 tgcggcgatt tatggactgg caagaattcc ttctataaat tcttggccat tactgaataa
     5161 aataggtttt tgccaaagaa tcagtttttt ctttaattat gtgcccattg cctggttgag
     5221 tatcttgcat ctcaaacagt aaaacttgag ccgtcaaatt gaacagtcag cggatacttc
     5281 atccggtggc tgttttttgt ttttctgaaa tatatatttc cacagttttc ttgtatagct
     5341 tctgttttag agaagaaagg agctatattt tgtgctacaa tcttaaaaag gtagagagaa
     5401 gaccaaatgg aagacagagg agccactaaa atatgagaaa catcaagttg acaatcgaat
     5461 atgacggaaa aagatattca ggatggcaaa gacttggaga tgacgataaa accattcaag
     5521 gaaaaatcga aaaagtattg catcagatga caaatgaaga gatagaaatt attggatcag
     5581 gaagaacaga tgctggcacc catgcgcgag gacaagtagc aaattttaaa acaaacacag
     5641 aaattggact ggcagaaatg atcgacttta tgaatcgtta ccttccgcgg gatattgtca
     5701 tcaaaagaat cgaagaaatg cctgagcgct tccattcaag atataatgcg gtgggcaaaa
     5761 aatacagcta ttatgtttgg aacaacgtga ttccttcagc atttgagcga aaccacagct
     5821 tttatttccc tcaagagttg gatatggaca agctgaatgc agcttgcgaa aaattagtgg
     5881 gtacacatga tttcattggt ttttctgcat tgaaaaaatc aaaaaaatcc acagtgcgta
     5941 cgattgatga attatctatc gaacgaaagg gagaaatgct tcattttact tttgtcggag
     6001 acggattctt atataaaatg gtgcggatta tcatggggac attactagaa atcggcacag
     6061 gtgccatgcc gatagaaaca atcgatgaaa tatttgaaag caaagtgcgg caacatgcag
     6121 gagaaaccgt ccctgcacaa ggtctgtttt tggatgaagt ttattatcga taaaaatact
     6181 ttgataatgg atgaaacgca tgtttcacag tataaatatc gatgttaaat aatgtatgaa
     6241 gcaaattaaa gaaatataaa aaaagacttt ttagcctgtt tatgtgtaca gctaaaaagt
     6301 ctttcttaaa taaaaatcta gtgagaatgt tgtacttctc ttctcggttg tttttgttta
     6361 gaaaaagtaa atcgataaag taaaaataaa atcaaagtaa cgatagataa atatcttgaa
     6421 gcaattgcta gttttgtgta ctgataagta ataacgactt tatttatacc tggaggaata
     6481 gttaattcag ttgtgccaaa cttagataat tttgtttgta ctagtttgcc attaatagaa
     6541 gccacttgcc ctttataacg ataaataggt gtcgtcaaga cagtattttt attattcgaa
     6601 ttatctaatt caattgtaag tttgttactc ataaaatgag ttgttggatt tatttcctgg
     6661 ttacctagta aatattgatc attattaatc atatcaggat gatagattga ctctttgttt
     6721 gcataatcag tatgattgta attttcagct atagcaacag cgtcttcagg tgctataagt
     6781 gttaatgaat ctttgttagc atggatagaa ttgcttactc ctgagagatg caatattagt
     6841 gcaagaaacg taaagaccat tattttccat gaattatttt tagtagaaaa aatataatag
     6901 atcactatac tcatcgtata ggctataaat aaagaagaaa aaccatttaa ccgccagaca
     6961 aattgtagac tcttaactgg tgtatggtta aaaagttgcc aaggaaataa attggttgaa
     7021 caaaaaagaa caaatacacc aagaaagaaa ataaataaat catcagcagt taattttttt
     7081 ataaaaataa aagcaccaat caatccgaga aataagaata atccaatagt atagttgtta
     7141 tagttattat ttaaaatgtg tgtcagcata tcactaggag ccataccact taattctttt
     7201 cccagtggga ctttcaattc ttgtgctcta gattgttgaa taaaaggaat taagaatccc
     7261 gcacttaaaa aaaaggtgag gacggtagct tttaaaagac ttaagagtct agcgattttt
     7321 tggtcccaaa aataaaaaga aagaaacaac gttcctccaa taaatacaga aaccatagcg
     7381 acagaaagta aatgagtata cactactagc gtcatgccaa tagacaacca gtaccatttt
     7441 tggtaatctc taatataaat ttcgtaacac cccattagta tcaaaggcaa aaatgttaaa
     7501 gcgacagctt ctcccaatga agcacgatgg aatatgtcaa tggcacgata ggcggaaaat
     7561 gtataaataa tcgaaaataa cagcgaaata taacgattgt tttttatttg tttcatagaa
     7621 aaataagaaa cgaccattgt taaaaaagtg ataaaaaaat agtagatatt ataacttaaa
     7681 acaagatttc ccatcatttt ataaaataaa aaagctggat aaagagtaag ccatggataa
     7741 aaaatattca tcattgttcc atgatgatcg aagttattga agtttactgg agaactccat
     7801 acattatcta gtcctatgat tcgagataaa tgaaagtaag tatcctgatt gacactatga
     7861 tagattccca tagacatgaa tagagggatg acgtaagcaa aacttagaaa taacataaaa
     7921 atgatatcta ttatttggtt ttt
//
LOCUS       DAZUMT010000122         7772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000122 DAZUMT010000000
VERSION     DAZUMT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.126"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(206..904)
                     /locus_tag="ACG8FM_002543"
                     /pseudo
     CDS             complement(206..904)
                     /locus_tag="ACG8FM_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            893..1159
                     /locus_tag="ACG8FM_002544"
     CDS             893..1159
                     /locus_tag="ACG8FM_002544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5095161.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKGFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1335..1904)
                     /locus_tag="ACG8FM_002545"
     CDS             complement(1335..1904)
                     /locus_tag="ACG8FM_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5095162.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1978..2301)
                     /locus_tag="ACG8FM_002546"
     CDS             complement(1978..2301)
                     /locus_tag="ACG8FM_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095163.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2455..3267)
                     /locus_tag="ACG8FM_002547"
     CDS             complement(2455..3267)
                     /locus_tag="ACG8FM_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095164.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3594..4469
                     /locus_tag="ACG8FM_002548"
     CDS             3594..4469
                     /locus_tag="ACG8FM_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5095165.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4483..4806
                     /locus_tag="ACG8FM_002549"
     CDS             4483..4806
                     /locus_tag="ACG8FM_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095166.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4803..5555
                     /locus_tag="ACG8FM_002550"
     CDS             4803..5555
                     /locus_tag="ACG8FM_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS5095167.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVCLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5545..7716
                     /locus_tag="ACG8FM_002551"
                     /pseudo
     CDS             5545..7716
                     /locus_tag="ACG8FM_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2316 a         1532 c         1398 g         2526 t
ORIGIN      
        1 tttacttctg tagaatatat actttgttgt tagataagtt gcacatgttt atagaatcta
       61 aataataaat ttttatgtac cgcccctcaa agtgaactga accccaaaag ttgaactatt
      121 taatggactg tttccgatat tctactggag atagtccatt aaatagttca acttttgggg
      181 ttcagttcaa agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt
      241 catgaaaatc ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca
      301 tgacgtatac tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat
      361 ttcctaaagt attataagct tgatttacgt ctggtctata aatcccatca gctcttactc
      421 tagctagttc ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag
      481 caatgtgcca tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt
      541 gggcgtaatt tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa
      601 ttactggttt atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat
      661 aggctagttt ttctttactt acacccattt cagaaagata accgtaagga tctgtatgat
      721 cgccccaaat attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag
      781 tgtctaatgt taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata
      841 ctttatagtt cttttcaaaa gtttctttat catgtgtatg ttgtaactcg atttgcacag
      901 gacttttggc tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa
      961 aacaccaaca ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga
     1021 agatatcaaa ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat
     1081 ttctcccgaa gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat
     1141 aaaaaagcac tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt
     1201 ttatttataa gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat
     1261 cttaaaggcg ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg
     1321 cgaacatggt tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc
     1381 aattgagatt gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt
     1441 tttcttctta actggtagtt gattttccca gtttccatca actcgctaag catatccacg
     1501 actccgccaa atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga
     1561 ttttttgctg taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct
     1621 tccatccctt cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt
     1681 tcttttggtt ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag
     1741 agcgtttcgt attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata
     1801 tatacgccag ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact
     1861 tctgtgatcg aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta
     1921 ctaatcgctt tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta
     1981 tcggtttttg cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg
     2041 atggtacaga tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat
     2101 cactagatct gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt
     2161 tcgatgtctt agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata
     2221 gcgaaaaaaa aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg
     2281 gagtgttttc agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat
     2341 cgctagccga aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg
     2401 ataataacga acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc
     2461 ttggtaaagt acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca
     2521 aaacagcgaa tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc
     2581 tatccgtttc caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata
     2641 atttttttct tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc
     2701 aaagaatagt gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt
     2761 caataattgg atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg
     2821 aaaatctttc aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg
     2881 tgctttttta taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg
     2941 cgtagaacga tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca
     3001 aaacgggggt gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc
     3061 ataaactaac tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc
     3121 caaacgtttc agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc
     3181 agcttttcgc cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt
     3241 ttttgataat agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg
     3301 tttgatcaaa atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat
     3361 acgcaaatct tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa
     3421 cgaatgtact ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca
     3481 ttacaatgaa ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag
     3541 aagaatccaa acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca
     3601 aacgttcaac tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg
     3661 aagatattcc cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg
     3721 gaaccttcag cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt
     3781 ccgtatacaa ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga
     3841 tcttgttagc agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag
     3901 caatggccga gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt
     3961 ttgtcttagc tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac
     4021 agctcgatcg ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt
     4081 tgctactttt ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt
     4141 atgaaataga agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat
     4201 cttatgcctt gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca
     4261 gtccacgagg aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga
     4321 gaaattacgt cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc
     4381 tgatttttta cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg
     4441 ttgcacaaat tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat
     4501 tatttcatta attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta
     4561 ttcaacaatt ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc
     4621 ttgatttcat taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc
     4681 gtttatcgca aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct
     4741 ttacctctcc ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga
     4801 ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag
     4861 gatgccatga aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa
     4921 aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc
     4981 aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag
     5041 gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt
     5101 ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat caagaagaag
     5161 aatgttctcc tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag
     5221 acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag
     5281 gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg
     5341 gcgatgagaa agcttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct
     5401 ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa
     5461 cggtttgtct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat
     5521 gttcattgcc aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt
     5581 tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa
     5641 aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct
     5701 aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat
     5761 tttccttcga agacattttc catattatgg atcttacagt ttttttcgtc agtcacagag
     5821 gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt
     5881 ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac
     5941 gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat
     6001 ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa
     6061 tttttctcca tccggttgtt gtcgggaaac actcgattcc agtcattgac tacagttctg
     6121 cttatttgtt tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa
     6181 tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac
     6241 acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa
     6301 cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga
     6361 aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg gatagaagat
     6421 caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc
     6481 gttatagaag acaaagaaaa cattacattg accctaaaaa aacaagtttt cttaccttat
     6541 ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac gctttactat
     6601 ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta
     6661 caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat
     6721 gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag
     6781 gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc
     6841 taacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact
     6901 ccaaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac
     6961 aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca
     7021 aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc
     7081 attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt
     7141 ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca
     7201 accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact
     7261 atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat
     7321 aaaatcgtct tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta
     7381 ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact
     7441 aatacgattg catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc
     7501 gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt
     7561 ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc agatccggta
     7621 aaattcgata aaaaaaccta tttagaaata acgcatataa taagaaaaat ggtatacaaa
     7681 aaaattctga caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact
     7741 tctggaatgc cgagaaccag ctgattgagt at
//
LOCUS       DAZUMT010000123         7760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000123 DAZUMT010000000
VERSION     DAZUMT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.127"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG8FM_002552"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG8FM_002552"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS5095168.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACG8FM_002553"
     CDS             complement(2032..3285)
                     /locus_tag="ACG8FM_002553"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS5095169.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACG8FM_002554"
     CDS             complement(3291..4214)
                     /locus_tag="ACG8FM_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS5095170.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACG8FM_002555"
     CDS             complement(4435..6927)
                     /locus_tag="ACG8FM_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS5095171.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACG8FM_002556"
     CDS             complement(6934..7401)
                     /locus_tag="ACG8FM_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS5095172.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="ACG8FM_002557"
     tRNA            complement(7537..7610)
                     /locus_tag="ACG8FM_002557"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="ACG8FM_002558"
     tRNA            complement(7668..7741)
                     /locus_tag="ACG8FM_002558"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DAZUMT010000124         7600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000124 DAZUMT010000000
VERSION     DAZUMT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.128"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACG8FM_002559"
     CDS             complement(137..427)
                     /locus_tag="ACG8FM_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095173.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACG8FM_002560"
     CDS             complement(545..805)
                     /locus_tag="ACG8FM_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095174.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACG8FM_002561"
     CDS             complement(942..1355)
                     /locus_tag="ACG8FM_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS5095175.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ACG8FM_002562"
     CDS             complement(1811..2296)
                     /locus_tag="ACG8FM_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095176.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="ACG8FM_002563"
     CDS             complement(2435..2650)
                     /locus_tag="ACG8FM_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS5095177.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="ACG8FM_002564"
     CDS             2874..3674
                     /locus_tag="ACG8FM_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5095178.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="ACG8FM_002565"
     CDS             3693..5561
                     /locus_tag="ACG8FM_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5095179.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="ACG8FM_002566"
     CDS             5554..6045
                     /locus_tag="ACG8FM_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS5095180.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /locus_tag="ACG8FM_002567"
     CDS             6033..6587
                     /locus_tag="ACG8FM_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS5095181.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="ACG8FM_002568"
     CDS             6605..7600
                     /locus_tag="ACG8FM_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS5095182.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga
//
LOCUS       DAZUMT010000125         7463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000125 DAZUMT010000000
VERSION     DAZUMT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.129"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2423)
                     /gene="pglX"
                     /locus_tag="ACG8FM_002569"
     CDS             complement(<1..2423)
                     /gene="pglX"
                     /locus_tag="ACG8FM_002569"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFS5095183.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSV"
     gene            complement(2487..6158)
                     /gene="brxC"
                     /locus_tag="ACG8FM_002570"
     CDS             complement(2487..6158)
                     /gene="brxC"
                     /locus_tag="ACG8FM_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFS5095184.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(6183..6758)
                     /locus_tag="ACG8FM_002571"
     CDS             complement(6183..6758)
                     /locus_tag="ACG8FM_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFS5095185.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(6758..7357)
                     /locus_tag="ACG8FM_002572"
     CDS             complement(6758..7357)
                     /locus_tag="ACG8FM_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFS5095186.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         2206 a         1411 c          986 g         2860 t
ORIGIN      
        1 acagatcctt tagtttcaaa aattgcctga ttctgcaatc taaaagatac ttgtccagat
       61 gaaagtgtcg accaagtaat tcccttttta aaatagtaat taagattttg tggtctagat
      121 tttaacttcc cattttcatt aacataattt tttatttcat ctccatcatt ctcccagtta
      181 accacgtatt cattgtttcc ataccatttt ctaaaacgac cacctttagt tgtaggaaac
      241 catttttttc tagacttttt agcgtcctct aatgttttag catcaaactt tattttgtta
      301 aattctactt caaaccaata ccgcaaaaat ttattgttat ttccggttgc caacccttgt
      361 tttgcatctg caacatcgtt agttaatttt ccttgagcaa aatcagtgat tacgttatcg
      421 cttgcccagt aagcaatcgg cattcctggg atttttttga agttgtcttg attagtacta
      481 taaaaataat cgcattcacc atgctgatga attgcttcta acgttttaat tttttgaact
      541 tccattccac ctttaaaatc tgataatttt atatagcttc catcttgttc atttgaattt
      601 tttatcgtaa acgtacaaat tggaacggtt gcttcttcaa aagctgaata ctccatttga
      661 atcaaactag aaatgtttct ttcattaatt aaaaatgagc gtaacttttc atagctagaa
      721 atgaacatcc aaacaaatgg cgtcataaag gaagcatatc cattatcaat cagcatttgg
      781 ctattcaatt taataaacac agaaaataga tcttttttga catcagaata gttatcattt
      841 acatattttt ttaatttctt atccatttta ttaagataag gcggattagt gactaccgct
      901 acatatttat caactagcat ttcagccacg tcaagaatgt gcaagtattt ttcttgtatt
      961 tccttcatcc cataaagaat atccatattc gtaaattcgt tgaattttga tatatactct
     1021 cttacttttc ctaagtattc tttagttatt gcctgattat ttttatcgac taacttaatg
     1081 attgatccaa gttctttccc attttcaaat tgatcaaaaa ttaactcttt gtattcattc
     1141 cagcgttcac tatacttatc attgttcaat tcactaatac gctcttgata ctcgtctgtc
     1201 actttaggag aatcaataaa ctctcttaaa ttcggcgata gacttttttt caaaaaaaga
     1261 cgatcgtatt gacgcccttt catcataatt gcaaaataag acaattgata cgctctttta
     1321 tcgatttcaa gaccatataa attgtattca acgattgcac gtgcagcatc acgctcagaa
     1381 tatcctaacg taagataaat ttccatcaat acatcgaagg cgtaaactaa aatatgccca
     1441 gaccccattg cgttatcgat aatttttagt tcttctggtt ttaaattact tacagatgtt
     1501 gcttgttcaa aagcatattt atctggaatt aagtatttca acttatttct taattcacta
     1561 ttgggataat gatctaacca atactttcct aaactattgt caaccatata acgaacgacc
     1621 caatctgtgg taaatagttg agttgctgcc ggaatatctt gttttttgac aatcccttta
     1681 taaatattca ccacttgatt atgtggctca ctattatagt actgatacaa ccacccaata
     1741 atctctacct gtccaccagc ttgaacatag aaatcatcct ctggaatact taaaagatca
     1801 gcgattacac catctttttt aatataagaa gctgtaaaca aaagttctgt ataatcttct
     1861 acttcttcaa atagctcagg taagtaatca ttcaattgat tacagatttt tataaaaatc
     1921 aattgataca gcttgtccat tgcttctgct gaattatctg ttttccaagt tctgacttgt
     1981 tgctgttcat tactagataa ttcttcaaag atatcggcat cataaatatc tgtaataata
     2041 tctggctcaa ttttcccact tatttcacta gataatacac gatacgtgtc aggtaaataa
     2101 tcatttacct ccataaaccg aatggcaatc aatcggttaa accacgtata agcaacctct
     2161 tcgattaact cttgaaaagc ttctgaataa gaagtgacag aagcacgttg ttttagttct
     2221 gcaatcaatt tgtttctttg cgctatttct tttccttgaa ttcttattcc tgatttttcg
     2281 atttcttgta aattgctatc aattttcttc acatcttcaa tttttgattc atcaattcct
     2341 aaacgttttg ctttagtcat tacttcatca attaattttc tacgggcatt gaccgcgaaa
     2401 ttctttatcg ctttcttatc catttttctt tcccttctat atgtaacctt tgaaaagaca
     2461 ggcacctttt tatgcctgtc ttttcactag aattgaatcg tgatttctgt attttcttga
     2521 agttgttcct tcagtttttg attaatttca gctgttaact catcaatatc tgctgctgac
     2581 ttaatagttt taacatgaac gttaagtgtt ctcattggta ctgttttctg ttgaatagta
     2641 ggttttacta tcgctatttc ttcagctttt gctgcagagt ctatttcatc tttttcagtt
     2701 tcacgaatca agtttgctgc ttcaatttgt ttacgtgctt gttcttcttg acgtctgcgg
     2761 ttttcttcat tatcgaaacg ttgattaaag atcaaatact gttctgctac gtttaatgat
     2821 aagaaaacaa gatcatctat agtattcgct tgttcaattt ttgctttcag ttcttgaaat
     2881 tcttggtcaa ctattggtac aaattgatcc ttaaaatctt ttccttctaa taatttaaat
     2941 gttgcttgtt tttcttccgt taatttattt aatatttcct gactttccaa ctctaacagt
     3001 tgattatatt gttcaataaa ttcttgaatc aactcattta gtttcatcat atctgaataa
     3061 ggatcagcct ttttcaaaat ggttttcatc tcatgaatca attgatcaat ttcttggcta
     3121 gaagtttgac gccgactttt ttcgattaat ctaattttat cttgtgcatt ctcccagatt
     3181 tttttctgag tactattttg cttgtggaaa tttttaattt tatcatatgt ttcagcccag
     3241 tcgaataatt catcgcgata ttttttgatt gtataaaaaa agtcttcaac atctgacgtt
     3301 tgaacaatta cttcaagata tttttttatt tgctcatatt cataagcact agggtaggta
     3361 taagtgtttt gtaaatcaaa ataagagcgt aaattgtcta attgaccttt taaatatttc
     3421 ttgaaatttt ccattgtatg ttcattgtta ttttcatcaa caatattacg actttgaaac
     3481 agttctctac cgatttcgtc aaccaatttt tttgcttttg gcgaaatcac cgcgcttggt
     3541 tcaattttta tcttttcact ttctcgtcgt ttttgaataa tcattacaat actatttgga
     3601 tcatccatcc gtgaaatttg tacattgtta taaagtaaat caattttacc agacatgaaa
     3661 agtttagcga tgcaccattc cacatcaagt tctgtgaatc catacggttg ttttgtttca
     3721 aaacgatcac gaattgtttt catcgtaatc gttaggtgat ttctcttttg atcattgata
     3781 aaattgaaaa tttcctgaac tgcttgctca tttgatggat cttcaataat ttcatttttg
     3841 ctttctttca ctaaatataa gatatcttct ttttctttag gcgcatcaat ttggtttaat
     3901 ttatggtata cttcttcagc cattttctga agtgctgatt ggaattttgc tttaaagtcg
     3961 gagcctgtca gttgaatttc tgcaccgtta tagaaaaatt ttgcagattt caacagttca
     4021 tttaattgat cgttgatact tgcacttagt tcctgtaatt caccagattt ttccgaacga
     4081 atcgcgtcaa agttctttac tacactcgaa taagtcgttg ttaaataaag attcattcta
     4141 gcatacattg ctatatcatc tttaaattct ttatctgatg gcaattctac gaataataag
     4201 ttacgggaac tcttaagcgc aagttctgcc tcttgacctg ctaaatcaga gtatggtgaa
     4261 ataaaattaa ctgtcatttc agcatttcca gcttgcttgt atggacgttg atctaacact
     4321 tgtgcataag taaacgtata ccggttttta aatttatttc cacctgacat gcctaatttt
     4381 tcataggcaa aactatttcg gtctagaacg ccgtcaaaaa ttcgtttaga tagttctacg
     4441 atcaaatcac tatctgtaat attttgattt ttaatctcac gagtaatttc ctgttcttca
     4501 tcagtttgga aaatataact atctaagttc ttccgcacaa agttttcctt aattaaaagg
     4561 tccaatgctt cttctactcg ttttttagtt tccaggcggt cttcgttgat atttcttatc
     4621 atcaagctcg taatattttc aacatttggt ttgatggctt gatcaacata tttaatcatg
     4681 aataaaattc gtaagacatt tacgttaaag ttatctatat cagttggtct ttgaatatga
     4741 gaattatttg tagcttgtgt aatcactcgg ctaattgctg tatcaatcca acgttctaac
     4801 gagtaataaa actgatctaa agaaaccaaa ctggttgtat cattttcctt atttcgttca
     4861 gcggcttctt taaacattgc tagtaatgaa cgttcaccag acgataagtt tgatccttga
     4921 tagctgtgat tacgaatctc ggttagaatt tgccccaata aattgaattg atagggaata
     4981 aatggataaa cttctgcgta attttcagca tcttggaaga gctttaattc aggactatca
     5041 acaaagagta atttattttt caattctgtt tgaacagttt caaattgtaa ggctaaagaa
     5101 tcattggcaa actctttttt cttcaaaata cgttctttaa ttacttcatc ggcatttgct
     5161 gaagataaac tcacagttgt ggcaaaacga tccttgatct tagaaaaatc attatcagaa
     5221 ttgttatcaa agtttgttac gtgatcaatc gcttcctgac ttgtcactaa tacccaagcc
     5281 ttaccttcac aaattcgacc caaatcttcc acaacggttt gtaactgcaa agtcaactca
     5341 cgattaccgc cgatatattg tccgacctca tcgactagaa aggcaatatg atggtctgct
     5401 tctttggaat caagatagct tttaactaga tttgcaaagt catcaatgct tattaaataa
     5461 tttcctgatg ctttttctaa aaaattatca atagcggctt cactcataaa atcgatttta
     5521 agtgccgctt ctttgatatc atcttggatc caatcaaact catggcgctc ttcttgccat
     5581 tttgccccat taatctcttc aaaagcagct ttgaatgcat caaacttttc ttctttaatt
     5641 aaccgacgtt caaaatctgc tagatgtgga atcgtgctta atccttgcat ttcgttaaat
     5701 acctttaaaa aaacattcac gattccgttt ttgtcttttt taccacctgc atcacttttt
     5761 gaatcaatat taaacagaat aacatctgta tttatttgtg agcaccgttc catcgttgcc
     5821 aaagtcagtg gatctttgat tttcccatct tctttgaagt aatcaatggc tttcttgtca
     5881 tttatttctc gatttgcgag caagtaagaa ataattttta agaaatgaga tttacctgaa
     5941 ccaaagaacc cctgaatcca aacacccatt ttatctgttg gtgtattgat actccgttgg
     6001 taggcatcaa aaattgtttt aaagtgtttt tgtagttctt tcgtaaccac gtattcttct
     6061 aattcttgtt gaacattttc atcctttgct tgaccgacct taataactcc ttgaatattt
     6121 cggtcaattg gttttgcaaa aatatcttta attagcattt aatctcctac cttccatccc
     6181 aactaaattc taaatgctcg ataataatta tcatctttat tggtattaag aattgtcaaa
     6241 cttaattcat catattcccc tggatacatc atcacaactg gtacgcgatc aaatacctga
     6301 tgcattttat ttaaaataga gtgcgctcta attaatggat ataattcgcc aattcctgtc
     6361 aggaacacaa ctgatttttc tggtgtatgg gcatccacat attcaaaaaa tacattttct
     6421 tcctcaggta aatccatttt caataaacga ccaatgcttt ccactaattt ttgtaaacca
     6481 cgtcgttctt ccatcttaat cgctttttca gtgaaaccgc gtttttctag atactctaac
     6541 ataatcacat agagattata aactttcact tcataattat caccaacaga tgttccagat
     6601 atatcattaa tataatctct tacaattaac tcgtctttcg gatcgtagtc aaagatataa
     6661 tagcctactt cattacctaa accacgattc aataaaaaat cttctttttg aattacttca
     6721 ttcaactttt ccaaatgctc ttgaatcgat aatgacatta taaaattcct cccagtgcta
     6781 gtacaatatc tttacgattc ttgtcaacta accattgaat caatcgttta tctaataata
     6841 tcggagtcat tcgtccatat tctttttccc ctctgactaa tcctgtttca attagattcg
     6901 tttgataagc ggcaataagc cgtttaatcg tataatcttt ccattttgaa atatcttctg
     6961 aacttctccg cttctctgta aaaaaagaac ggtaatcaac tggtgttaat tgcaaatttc
     7021 ctttacgtag attttcagca tatacctcag tcataaattc attaaaaaga tcatcaatca
     7081 taaggatgga aatcaaattc actaactttt gattatctat atttaactga ttgaaaagtt
     7141 gcaatccagc ttcatccagt tgactaactc tccttttcac gttctgatag atttcttttt
     7201 gacggttact gctcactgca aaaaaaatat tttcatgttc atttttctct ttaatttcta
     7261 tatctgtata tccttctagt cgtaattgaa tatataactt gaactctgaa aaccaaaagc
     7321 gtttagaaac tagaccagca gaatattttt tattcaatag attgcctcct aatcatctat
     7381 aattcaataa gtaaacaagt gttttttgct catcgttagc attcattttt tagtatctac
     7441 tatacaatac atatttatta tag
//
LOCUS       DAZUMT010000126         7353 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000126 DAZUMT010000000
VERSION     DAZUMT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.130"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACG8FM_002573"
     CDS             133..633
                     /locus_tag="ACG8FM_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5095187.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="ACG8FM_002574"
     CDS             730..2577
                     /locus_tag="ACG8FM_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS5095188.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="ACG8FM_002575"
     CDS             2580..3476
                     /locus_tag="ACG8FM_002575"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5095189.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="ACG8FM_002576"
     CDS             3526..3915
                     /locus_tag="ACG8FM_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5095190.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..6232
                     /locus_tag="ACG8FM_002577"
                     /pseudo
     CDS             3902..6232
                     /locus_tag="ACG8FM_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6340..>7353
                     /locus_tag="ACG8FM_002578"
     CDS             6340..>7353
                     /locus_tag="ACG8FM_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS5095191.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRT"
BASE COUNT         2468 a         1112 c         1578 g         2195 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg ggattacgtc
     6241 catataattg tgtaaaagtg aagcatcaaa aaagccattg atctcagtta ggagtacaat
     6301 gttttttgca gaaaacatac tcgaatagga ctgatatcaa tgactcaagt acattttact
     6361 tttgaaagcg aagaaattca agcaattata aacgaaagcg gtgcgaatga taccgcaaaa
     6421 acacttatga ctatcatgtt taatcagctg atggaagaac aacgaaatca atatattcag
     6481 gcaaatgcct atgaacgttc agaagaacgg caatcacaaa gaaatggtta ctacgatcgt
     6541 tcgtttacaa ctcgaattgg tacgttagaa cttcatgttc cacgaaccag agacggtaag
     6601 tttagtccta ctatctttga aagatatcag cgaagcgaaa aagctttgat cgctgccatg
     6661 attgaaatgg tcatttctgg tgtctccact cgcaaagtaa ctaaaacagt tgaactgcta
     6721 acggatggtg cgactgtttc taaatcattc gtttcaaatc tgatgaaaca gttggatcca
     6781 tttgtttttg agtggagaaa ccggagccta gaaggctcgg aatatccatt tttcatgtgt
     6841 gatgccctgt atatgaaagt aagggaaaat catcgtattg tctcaaaagg tgtatatatt
     6901 ggtatcggca ttgattctga tggacgacgt acgattcttg gttttgatgt tcaagacggc
     6961 gaatcggaag ataattggga taccgttttt caatcgttcg ttcaacgagg tttgttcggt
     7021 gtaaaactgg tcatttctga tgcacacaaa gggttagtta aggctgttcg caagaacttt
     7081 ttaggtgcga gctggcaaag atgccaagcc cactttttga gaaatatttt tgataagctg
     7141 ccaaagaaag tttcttctga tgtaaaagac gagttgaaga gtatctttaa agcctctgaa
     7201 cttgaattga cacgtgaaag aaaagagcac ttcttggaaa aatatggttg tgattcaaaa
     7261 ttaagtgctg cttgcgacat tttagaaaac ggctttgaag atgctattca aattttgtct
     7321 tttcctgaaa atatccgccg aaggattcgt aca
//
LOCUS       DAZUMT010000127         6706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000127 DAZUMT010000000
VERSION     DAZUMT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.131"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..573)
                     /locus_tag="ACG8FM_002579"
     CDS             complement(115..573)
                     /locus_tag="ACG8FM_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5095192.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(586..1917)
                     /locus_tag="ACG8FM_002580"
     CDS             complement(586..1917)
                     /locus_tag="ACG8FM_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS5095193.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1928..3370)
                     /locus_tag="ACG8FM_002581"
     CDS             complement(1928..3370)
                     /locus_tag="ACG8FM_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5095194.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3357..4121)
                     /locus_tag="ACG8FM_002582"
     CDS             complement(3357..4121)
                     /locus_tag="ACG8FM_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS5095195.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4225..5100)
                     /locus_tag="ACG8FM_002583"
     CDS             complement(4225..5100)
                     /locus_tag="ACG8FM_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5095196.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5355..5439)
                     /locus_tag="ACG8FM_002584"
     tRNA            complement(5355..5439)
                     /locus_tag="ACG8FM_002584"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5403..5405),aa:Leu,seq:caa)
     gene            complement(5457..5528)
                     /locus_tag="ACG8FM_002585"
     tRNA            complement(5457..5528)
                     /locus_tag="ACG8FM_002585"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5494..5496),aa:Gln,seq:ttg)
     gene            complement(5538..5610)
                     /locus_tag="ACG8FM_002586"
     tRNA            complement(5538..5610)
                     /locus_tag="ACG8FM_002586"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5575..5577),aa:His,seq:gtg)
     gene            complement(5672..5756)
                     /locus_tag="ACG8FM_002587"
     tRNA            complement(5672..5756)
                     /locus_tag="ACG8FM_002587"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5720..5722),aa:Leu,seq:caa)
     gene            complement(5777..5847)
                     /locus_tag="ACG8FM_002588"
     tRNA            complement(5777..5847)
                     /locus_tag="ACG8FM_002588"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5813..5815),aa:Cys,seq:gca)
     gene            complement(5893..5964)
                     /locus_tag="ACG8FM_002589"
     tRNA            complement(5893..5964)
                     /locus_tag="ACG8FM_002589"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5930..5932),aa:Gln,seq:ttg)
     gene            complement(5976..6048)
                     /locus_tag="ACG8FM_002590"
     tRNA            complement(5976..6048)
                     /locus_tag="ACG8FM_002590"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6013..6015),aa:His,seq:gtg)
     gene            complement(6051..6124)
                     /locus_tag="ACG8FM_002591"
     tRNA            complement(6051..6124)
                     /locus_tag="ACG8FM_002591"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6090..6092),aa:Trp,seq:cca)
     gene            complement(6131..6211)
                     /locus_tag="ACG8FM_002592"
     tRNA            complement(6131..6211)
                     /locus_tag="ACG8FM_002592"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6175..6177),aa:Tyr,seq:gta)
     gene            complement(6213..6288)
                     /locus_tag="ACG8FM_002593"
     tRNA            complement(6213..6288)
                     /locus_tag="ACG8FM_002593"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6253..6255),aa:Phe,seq:gaa)
     gene            complement(6324..6396)
                     /locus_tag="ACG8FM_002594"
     tRNA            complement(6324..6396)
                     /locus_tag="ACG8FM_002594"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6361..6363),aa:Asp,seq:gtc)
     gene            complement(6406..6478)
                     /locus_tag="ACG8FM_002595"
     tRNA            complement(6406..6478)
                     /locus_tag="ACG8FM_002595"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6443..6445),aa:Val,seq:tac)
     gene            complement(6494..6565)
                     /locus_tag="ACG8FM_002596"
     tRNA            complement(6494..6565)
                     /locus_tag="ACG8FM_002596"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6530..6532),aa:Glu,seq:ttc)
     gene            complement(6580..6669)
                     /locus_tag="ACG8FM_002597"
     tRNA            complement(6580..6669)
                     /locus_tag="ACG8FM_002597"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6633..6635),aa:Ser,seq:gga)
BASE COUNT         2024 a         1476 c         1089 g         2117 t
ORIGIN      
        1 cgataattaa caaaaatcaa aatgattaaa tctagtgatt gaaacatatc atttcaaaaa
       61 aattttgtaa attcacccat aaatatcacc ctatttaact tttttcatcg tacactaaaa
      121 ttcaaaacaa ctatctttaa tctttacttc ttggttttgt tttaataaat ttccttcaaa
      181 aatgcctgga gaaactacaa tgatcaccgt atctttttgt gcttgctcca acttcttcaa
      241 atctataata gctaacagtg tcccttcttt tacctcctgg ttactttcaa cccgtatctg
      301 aaacggactt ccttcgagtt caactgtatc taaacccata tggatcagta tttttctgcc
      361 agattcactt tcgatcgtta tggcatgtcg ggtaggaaat atattatcga ttattccttt
      421 aactggcgca tggatatatc catcatgatt cgttatagcg tatccaactc ctatcatttt
      481 tgaagaaaag acctcgtctt ttactgctgt taatggaata atttgaccac tagctacagc
      541 ttttagaatg tttcttgttt taaagaaatg cactttttat cacctctact ttaatgctgg
      601 ccaatatttt ttgtttactt tgatcatttc atctagtatt ttttttgcga ctctggcatt
      661 tggtacggtt tgattcaatg taaatgcttg taaagctttt tgataagaac cttcaaagta
      721 agcatctact agtaacttct ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc
      781 actgattggt tttcttagtg taattggctc cacaccagtt gcaccaacat atgccggtat
      841 ttcaacaact gcatctgacc gtagattcgg aatcgcccct tgattaggaa cgattagcat
      901 aaagcgacga cgatcatcat tcaacaagga aatagctata tcgacgatat attgtccatg
      961 ctccccaaaa ttaaagttca atacttcttc cacaataccg cggcgtattt tatcagccat
     1021 ttctttggta tttttctctc gtccatccat gaccatatta gcacgtgtat actctttgtc
     1081 agtatgctca acgaccatat caggatataa gtaatactct aagtaattat tcgggatatt
     1141 acttggaaaa tttttcgtga tgactgacat catttgaaag gttttttgcc aagttttatc
     1201 tcctatatta aaatcggcga cttgcatttc ttttgttatt agtttttcaa taatctctgg
     1261 cataatttct cgttgcaatt ctttatcata aatcgatgta taccagccaa agtggtttaa
     1321 accataatac gtaacgatcc aattcttacg atcataaccg taattcactg caatcgtttc
     1381 ttcaattgaa atagtcatat cacaggcatt aatcattttt acattcggat atttacgacg
     1441 aacagcttca gaaacgatcg actctggatt tgtataattc aaaatccatg ctttgggggc
     1501 atagtcttgt atatgatcga caatttccaa taaacctttg attgagcgca tcccataagc
     1561 aaatcctccg agtccacacg tttcttgtcc tactaatcca tgtttaagtg gaatcttttc
     1621 gtccttttct cgcatctcca atcctccaac acgaatttgt gagaaaacaa aatcgcatcc
     1681 tgtaaaagca agctttggat catccgttgc acgaatgcga atatttgaaa aaccttttct
     1741 tttcagcata aatttaacaa tcaagtacat atccatattt ctttcttcat caatatcata
     1801 taaacgtatt tcagataatg gcaaccgttt ttcattgttc aaaatggctt gaacgattcc
     1861 tggtgtatac gtactcccgc ctccagctat agtgataatc tgttttttca tcatcatctt
     1921 ttcctttcta ttcttctaat aatttattga attctgccat aacataagtc acttctaagc
     1981 cgatgataat ttgataattt ttttcagttg gtttaatgat cccgcttatc cctaattgct
     2041 tgattcgctg ttcctcgacc atggtgggat ccactacctc tacccgtagg cgtgtattac
     2101 aactatagac atccaggata ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca
     2161 tatattcata atttttactt gctaacccta actctgtttc ttctaaggtt acttcattgc
     2221 caaactctgc cttagcttca cgtcctaatg tctgaatgtt tctcttattg atcaaataat
     2281 aaaaagtaac gtaatacaag gcaaaaaaga aaattccgat tggaagaatc aaaatagcat
     2341 tggtagcgat tcgaaaattg atgagataat caacaataca tgctccgaat gtaaaaccaa
     2401 ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca
     2461 cgtacaacat aggagaaaga aacatgaaac taaattcaac aggctcagtg atatttgcaa
     2521 taaatgaagt tcctgcaccg cttcccatga ttcctttgac catctcttta ttttctttat
     2581 aagctgcttt atagatagct agacatatag ctggtatacc aaacatcatt actacaaaaa
     2641 aaactgataa aaacagtcca gcctgaggat ctccgttgat aaagcgtgtc atctctcctc
     2701 tgaaaacttc cttgctagga gatgtatagc ttcctaaatc gtaataaata tacgtattca
     2761 acacatggtg cagtccaaag gggatcagca aacgattcaa aaacataaat atccccacac
     2821 ctaatgctcc catatcaacg agcagttttg caaaattatc aatcccaatt tggatgagcg
     2881 gccagataaa gcccaaaaaa acagctgcga ttgtttgtag aatcatcacc atagtcaatg
     2941 ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa tttttgttct ttaaagcgat
     3001 tatacgtcca tgccgctact aaaccggaaa taattccacc aaaaatcccc atattcactt
     3061 ctgggtccat aatttctaag ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg
     3121 caggtattcc tttgtcctta tttttgacaa aaccgattgc aactcctata gcaaataaga
     3181 tatccaagtt tccaaaaacg gcatttccag ctgcaccaat tactgggatg ttcagcatat
     3241 cgtcagctga taaacgcatg atcaatcccg ctaatggcat cgctgcaact ggaagtagca
     3301 tcgctcttcc aagcttctgc acatttgatt taacattcat ttttattcct cctctattaa
     3361 atatcctcat atgttactaa ctcaatcaaa ggatcatttt tcactttttg agcaaatgct
     3421 gaatgaatta aaaattcgac ttggtgggta cgaggataca caaaacttga accatttaac
     3481 aattcctgat ccaaatacgc tgcgtgacac atgacttcga tcgttttatc ttctctcaat
     3541 atcatgagta aacttgccaa atattcttcc acactcgtat tgatttgtcg ttttttttca
     3601 tcagtttctc ctacgacatc gaaacaaggc tcaaaataat cgacatgacg aacttcatct
     3661 tttcgttcaa agttgctccg aaccggcaaa tcatattttt ttgctaatgt aacaactacc
     3721 tcctgatttt catccaaagt atgagtatga tgatgactgt ctaaatgagt tggtatgatc
     3781 cccgcatgat aaattttttg aatctgagca ttccattcct gatacagttg atctttgtca
     3841 atgttaaatg gatgctgata ataagataag gatctgaact ctccgctttc gataagagta
     3901 tcgacagttt tgaggagtgg tttgccacac gtcaatgtta aatggactcc tacacctagc
     3961 ctaggcattt cctttcttag tttcacagca tgctcgaaac ccggcatatt tgccatcaat
     4021 gtagttgatg tcaaaattcc tcgctgataa gcatccataa tcccataatt cacaccatgg
     4081 ctatagccaa aatcatccga attaaaaatc acttttccca tttcattctc cttctttttt
     4141 ctactaaaat aggcgcaacc aaataatcat agtccattta ctatgtgtca tttggttgcg
     4201 cctatagata gttacgccct tattttaata gattcttgct agatgaattg ccaaatagat
     4261 gatttcattt tcttgaatgg caagttgaaa ttgctgttcg atgatatctt taatctttag
     4321 tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta aaaaaaccaa tattttctac
     4381 tggcgtttct tcctgataaa tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg
     4441 atgataagtc agtgattctt tatcataaac gtttgaatag gatttttcta gatgggacaa
     4501 aatcgtggtc gtcatttcta ctatttttaa gctctttaaa ctattaggct ctcctagctc
     4561 agcattaatg atatgcatag tgatgaatcc tacttcattt gaccctaact cggtttgata
     4621 tcgatgattg attcgctcga ccacttctac tgctatatca tattcatttg gataaaggaa
     4681 tctcatttct ggcaacataa ttgctgtgac atctctatct tctctttttc gttgaatagc
     4741 aaaataaata tgatctgtca atgtaatata gatatttgaa tctaatggag aggagatcct
     4801 agctgttatc agttcaatca cttcttccgc aaaatccata aattcgattg gaatctgcga
     4861 gactatctca ctgaatcgtt tgatcttttc tttagaatct aatacaaata atttgtcagc
     4921 atcctcaaca tcaaatggct tactcacttt agcagaaaag ccaatccctt tccccatcgc
     4981 aattttttct attccatcgt catcgatcaa tacagcatta tgattcagct ttttcatcac
     5041 atacaccgtt aattcacctc ctctatatga taaaacaaaa caacttgcag actccttcat
     5101 acatattttt gtctccaaac aatttttaaa gacaaaaaac gacctaagaa agaacccttt
     5161 cttaggtcgt gcctaattga atagtagcac cctacataat ttacaaaaat agtttaacac
     5221 ataataaaag cgttatcaat atctttttta ctcgttttat gataatactt gtatcgaatt
     5281 gatttggagg taattacata aaaacaaaaa aaatatagca actctctata agaaagttgc
     5341 tatattgata attataccgg cggccggggt cgaaccggca cgtcctcaac ggacactgga
     5401 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca aatttaaaaa taataactgg
     5461 ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct
     5521 ataccccaag aataaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat
     5581 ccggtgcgtt aaccacttcg ccacattcgc catcataaaa gatgttattt aatttctcaa
     5641 taaaaaacta agatgaagac atcttagtca ataccggcgg ccggggtcga accggcacgc
     5701 cctcaacggg cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaagt
     5761 tgatttatta gtactaaggc ggtaaccgga tttgaaccgg tgatgaaggt tttgcagacc
     5821 tctgccttac cacttggcta taccgccatt atactattat caatctataa aataaagaat
     5881 aaattataga aactggggta gctggattcg aaccaacgca tgagggagtc aaagtccctt
     5941 gccttaccgc ttggctatac cccaagaata aaaagggcga atgatgggaa tcgaacccac
     6001 gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tggcaggggc
     6061 agcaggaatt gaacccacac taacggtttt ggagaccgct gttctacctt taaactatgc
     6121 ccctataaaa tggaggagag tggattcgaa ccactgaacc ctaaggaacg gatttacagt
     6181 ccgtcgcgtt tagccacttc gctactcctc catggtggcg cgggacagaa tcgaactgcc
     6241 gacacacgga gcttcaatcc gttgctctac caactgagct accgcgccat attttaaaat
     6301 tattaattga ataacaagaa cgacggttcc gacgggaatc gaacccgcga tctcctgcgt
     6361 gacaggcagg catgttaacc cctacaccac ggaaccagtt cattatggag gttaacggga
     6421 tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc cagctgagct aaacctccaa
     6481 cattcaatta aaatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt
     6541 gtcttaaccg cttgaccaac gggccaggta aatttgctac ggagagtaag ggattcgaac
     6601 ccttgagaca gtttggcacc atctacatga tttccaatca tgctccttcg gcctctcgga
     6661 caactctcca gagtgaaaga agctcaatac tctttctcaa aatgag
//
LOCUS       DAZUMT010000128         6644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000128 DAZUMT010000000
VERSION     DAZUMT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.132"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8FM_002598"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8FM_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8FM_002599"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8FM_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS5095197.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8FM_002600"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8FM_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8FM_002601"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8FM_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS5095198.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8FM_002602"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8FM_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5095199.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8FM_002603"
     CDS             3582..4271
                     /locus_tag="ACG8FM_002603"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5095200.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8FM_002604"
     CDS             4285..5787
                     /locus_tag="ACG8FM_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5095201.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8FM_002605"
     CDS             5800..6276
                     /locus_tag="ACG8FM_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5095202.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8FM_002606"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8FM_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZUMT010000129         6368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000129 DAZUMT010000000
VERSION     DAZUMT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.133"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..739)
                     /locus_tag="ACG8FM_002607"
     CDS             complement(<1..739)
                     /locus_tag="ACG8FM_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5095203.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGA"
     gene            complement(859..1506)
                     /locus_tag="ACG8FM_002608"
     CDS             complement(859..1506)
                     /locus_tag="ACG8FM_002608"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFS5095204.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1590..3017)
                     /gene="uxaC"
                     /locus_tag="ACG8FM_002609"
     CDS             complement(1590..3017)
                     /gene="uxaC"
                     /locus_tag="ACG8FM_002609"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5095205.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3091..4170)
                     /gene="uxuA"
                     /locus_tag="ACG8FM_002610"
     CDS             complement(3091..4170)
                     /gene="uxuA"
                     /locus_tag="ACG8FM_002610"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5095206.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPACEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4173..5804)
                     /locus_tag="ACG8FM_002611"
     CDS             complement(4173..5804)
                     /locus_tag="ACG8FM_002611"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5095207.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6114..>6368
                     /locus_tag="ACG8FM_002612"
     CDS             6114..>6368
                     /locus_tag="ACG8FM_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5095208.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHL"
BASE COUNT         2015 a         1308 c          864 g         2181 t
ORIGIN      
        1 ccgcaccaat tatagaaagt aatgccctat ttttaactaa cccttttaaa gattttaaca
       61 atgaaacttt gtattgagat tggtcaattc ttactcgttc agtagttagt ttataacata
      121 ttaaataaca aataagtgca catatcgaaa atattcccgc aacaattgta aaagttgatc
      181 caccacgaac tatttgagcg cctgaatcat ctcttgtata aataaataat ggtgttaata
      241 caccaatgac caaacttgct aaagtagctc caacatttcg gaatgtcgat agcattgttc
      301 tttcttctgt aatagaagta atagctgatg ccattgatcc ataaggaata tttatagatg
      361 tgtaaaaaat actcccccat aatatatacg taacatacat gtatactacc ttaacgctca
      421 tagaagctga aatcatagaa gtttgataca ttaaaaaact tgctaaagcg actggaccac
      481 tcattcttaa aatccacggt ttaaacttac cctgcttagt tgctttcgat ctatcaacaa
      541 tacgccccat agttacatca gtaactgcat cgataaatct tgctactaaa aataatgttc
      601 caacggcagc tccgcttatc cccaatactt ttgtataaaa gatcattaaa aaagaactag
      661 caaaaatgaa agtaaaatcg tttccaaaat ctccaaacat atatcctatt ttgtctctta
      721 taccaaattt tctttccata caattctctc ccttctgttg ttaagcgttt tcattctgat
      781 gaaagtacgt actatactct gaagcgtaaa gagtcagtat ttatttttta catttcagag
      841 tattatttgt tttttctcct aataattctt tatctcataa aacttatcta tatatttact
      901 agctactcga cttacttctt caaaatcacc tttttcaaca gaagccaata aattcccacc
      961 tacaccaact gcaacaactc ctacttcaaa ccaatcttct aagttctcta tacttactcc
     1021 gccagtaggc attatattta cgtgtggtaa tggagcttga taggctttta caatggatgg
     1081 accaaaagca gaacctggga atagtttaat gatatctacg ccagcagtaa gtgcctcctt
     1141 tatctcagta attgtcatac aacctggtaa ataaggaatt tgatataaat tacaaatctc
     1201 agctgtttgt cgatcaaaag atggactaac gatatattct gccccagcca taattgcgat
     1261 acgagcagta aacgcatcta aaaccgtacc ggctccgatt attacatctt tttgatcagc
     1321 atatttttta gcaatttctt tgattatttc ttcagcatta ggaactgtaa aagttagttc
     1381 aatcccttta attccacccg caataattga atttattgct ttaatggcct catccttatt
     1441 tttcccacgt acaaccgcaa tgactccagc cgcttctaac ttacttaaaa tttctacttt
     1501 tttcatatct atctccttcc tttatggata catactttgt ctagctgtac taacttacaa
     1561 agaataaatt taagctttta tttcaagtat tattttctac ttccgatttc aaaaaaatta
     1621 aaataattat acgcattacg ataacaaata ttctccacta tctgactaag atacatttca
     1681 tcatctggaa ttcggcctgc tttaacccac tcgcttagta attgacataa tactcgacga
     1741 aaatactcat ggcgaggata gcttagaaaa cttcgagaat cagtcaacat acctgtaaaa
     1801 ttcgacaaca aactttgttg tgccatcgta gtcaactgtt gacgcattcc ttcagctgta
     1861 tcattgaacc accaagcaca tcccaattgg attttttgaa tcataccccc ttggaagctt
     1921 tgcattgctg ttgctagtgg aagccaatca gttggattta atgaatataa aattgttttt
     1981 ggtaagcacc cactcttttc agcggtatcg aacaatgcta caacattttg tgtcaaacta
     2041 gcttgatccc ccatcgaatc accaccgaca tctactccaa tttgttcctt taatatactt
     2101 gaattatttc ttaaagcatt taaatgcatt tgcatcgtcc aaccatatct tgtatactca
     2161 gacatcaaat gtaattgaat acctgtaaca aattgtgcaa tttctgataa atggaaatcc
     2221 gtttttcctg ctaaagcttt tattaacaga ttgtcgagta ttttttcatc tgctatttca
     2281 taaaaataag tattcaaacc gtgatctgct aatcgacagc caatttgatg aaaatgatcc
     2341 actcgttggc tcaatgcttg cttcaaccca ttaaagtctt tgattggtat agccgctgtt
     2401 tgactaagtt ttaaaatata gttcgaaaag cctgatgatt tgatactcca gaccttatct
     2461 ggacggaaag taggtaatac cttgaaagaa ttatcctgtt tagaaagctc agtatgatac
     2521 cacaagtcat ctgtaggatc atccgttgta cagatggctt tcacatccat catctccaac
     2581 aattctttcg ggcgatagga atccgtttga attttttcgt tagcacgtcg ccaaatttct
     2641 ggtgcattct tctcagaaat tgtcaaatca atatcaaagt aacgttgtaa ttccaaatga
     2701 gaccattcaa aaataggatt acctggcgcc ttctccactg tttttacaaa agctaaaaat
     2761 ttttcataat catcacctgg accactgatc aactcttctt ctataccatt agctcgcatc
     2821 aatcgccatt tataatgatc tccagctcct ttatcattta accatacctg agtcaaattc
     2881 ttgtattgct tattttctag caaatatgct gggtctaaat gacaatgata gtcgataatt
     2941 ggttggtgtt ttgcaaattt atgaaaaagt cttttagcta tttcattctc taataaaaag
     3001 ttcttatcta aaaacatatt taaacactcc tttccttccg ttagttttta gaagagaaaa
     3061 tttttcctct aaataaactc ccttatataa ttatttactc gccccctttt caagcgcttc
     3121 ccaaatacca tttaaataaa ctgcacctag agctcggtca tataatccat aaccaggtct
     3181 accggtttct ccccaaatca tccgaccatg atctggacga atatagccat taaatccatt
     3241 atcgtacatt gtttttaata tcttatacat atctagagaa ccatattctg atttatgcgg
     3301 cgcttcgtaa aaatcgagtc cattgtcact taaatatttg atattacgaa catgtgcaaa
     3361 aggaatccga tcccgttgac aaaattcaga tagaatttca tagacatcat tatctggtcg
     3421 ttctgcaata cttccagtac ataatgtaat accattagaa ggagaatcca ctacattaca
     3481 caaccaatct aaatcgtcac gatttttaac aatacgaggt aatccgaaaa tggaatatgg
     3541 tggatcatct gggtgaactg ccattttaat cccaacttct tcacatgccg ggataattgc
     3601 tttgagaaaa tatgcaaagt tctctcgtaa tttctcttca tcaacttctt tataggcttc
     3661 taataattgc ttaatttgtg ataaccgatg aggttcccaa cccggcaatt caaaattatt
     3721 agtagaattt tctacttctc taataatttc ctccggatca gtagggatat tttttgaaat
     3781 ataggctaac gtagaagacc catcatctaa acgataatcc aaatccgatt ttacccaatc
     3841 aaatactggc ataaaattat aacagataac ctcaatccca aattctgcca aattgcgaat
     3901 cgtctgttta taattctcaa tgtattgatc tcgtgttggt aaaccaattt tgatatcatc
     3961 atgaacatta acactctcaa taactttcaa ggtaagtcca ttggcttcga cttcatcttt
     4021 taaagattta attcgttctc tcggccaaac ctctccggct ggtatatcca ttaatgttcc
     4081 aacaattcct ttcattgctg ggatctgacg aatttcttgt agtgagattt tttcttcatt
     4141 tgccccatac catctaaaag tcatttccat agttactttc ctccaacttc tgttatatat
     4201 ttattgattg ttttacgaac tgcttgagga cctactagca tttcgcaaaa gattttttca
     4261 gttttctttc ctaccccaac ttgatataaa tctaaaccaa agatttcttt attttctaaa
     4321 atttcctgta ccgcacaatg aatctttgaa ggatcttgtt cacctaattt caatactgat
     4381 aatttatcct gcaactctac caataacgga tcaggacttg gcacaaaatt ttccccttga
     4441 tcatcgactg ccagtaaata acgtaaccaa gcagcaatag cgataggaat aaatgttaga
     4501 ttttctgcat ttccgtcttt ttcgacatac tttttaattg tttcaccata gcgaatcgct
     4561 actttttgag aagtatctgt tgcaattcgt tgtggtgtat ctggaatata gggattaggc
     4621 aaacgtttat taattacttc atcaataaat tgttttggat caataatgcc tggattttca
     4681 actactggta aaccttctaa ataacctatt cctttgatta aaccaaccaa atcatcatcc
     4741 tgcatctctg ctgaaattga ttgatatcct agcagacaac ctgttactgc caaagcagta
     4801 tgaagtggat tcaagcttgt cgtaaccttc atgatgtctg ctttatcaac cgtctctcta
     4861 tctgtcataa tcacaccagc cttttccaat ttaggccggc catttggaaa actatcttca
     4921 ataactaaat aatgaactac ctcagtatta gtgaaagcag caatattggt atgttttttt
     4981 gtgtgaatga tatctaaatc ctcgagcccc atcatcaata gatcttcctt tacattctct
     5041 gaaggatttg gcgtaattcg atcaatcata ctccaaggaa aagtcacttt ttctttatcc
     5101 tttaaataag caataaattt ttcagaaaca tatccttctt tttgccaagc ttctgcaatc
     5161 gtaaaaatac tatcttgaaa acgttgacca ttctgtgaga aattatcagt agaaaccatc
     5221 gcaatcggca actctccagc attaaatctg ctatataaca aggatgttac aatactcatc
     5281 gtatgttgag gattgctagg tccagatttg atatcagctt tgactaccga aaagaattgc
     5341 cctttagaat cttttaaact gtaacctttt tcagtaatag taaacgttgt aaactgtaat
     5401 ttaggatctt caaaatattt aaccactctt tgatagtccg ctatattctt cggattgcaa
     5461 tataaactat cagccgttac tgccaaaagt ctttttttga gttcaccatc tccactcata
     5521 acaatttcca aaatgtcatt gttatagtca tgataagctt tttccactac ttcttcatca
     5581 aatgtttcac acacaactac tcctgtttct aattcatttt gatctgctaa gcattgagca
     5641 atttctgcat gaaatcctcg ataaagattt cctccaccaa aatgaagcca tactggatgt
     5701 tgctggcctt cttgcttaac ttctttgaca tcaaaacacg gcagtagaat ctgcatttca
     5761 ttgaattctt cgatagaatt ttgccaattt ttaatcgaaa ccataattaa cctcctcaaa
     5821 ctgatatgtt agttttgata cattttatat tttaaaaccc attgtaaccg ttgtcaatat
     5881 aaaaagggaa taaacttgct tttttctacc atatcagaat gattgtattt tttattcaga
     5941 aaaataaaat actccttctt tttggactga ttttatcgaa acttattttt tctttttttc
     6001 atgtattctt ttatttttat aactagtgta ttacttcaaa atttgatatt atatggcatg
     6061 aacagaatta aaatatcagt taaaataaaa gttaaggagc attgtgtatg acaattaaaa
     6121 ctagtctcca aaatcaagct taccaatcta ttcgccgaca aattatctat gctgacttag
     6181 aacctggaaa aaaagtatca gaaaaaaaat tagaagaaac actttctatt ggtcgtacac
     6241 caatacgtga agcactgatt caactccgac aacaaggtct tgttgataca attcctcaaa
     6301 gcggaaatta tatctcatta attgatttag attcggccaa aaatgctcga tttgtcagag
     6361 aacatttg
//
LOCUS       DAZUMT010000130         6223 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000130 DAZUMT010000000
VERSION     DAZUMT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.134"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..180)
                     /locus_tag="ACG8FM_002613"
     CDS             complement(<1..180)
                     /locus_tag="ACG8FM_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HFS5095209.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSELAPALY
                     MCDEKIVLPKVSESNY"
     gene            complement(192..2009)
                     /locus_tag="ACG8FM_002614"
     CDS             complement(192..2009)
                     /locus_tag="ACG8FM_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFS5095210.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2023..2787)
                     /locus_tag="ACG8FM_002615"
     CDS             complement(2023..2787)
                     /locus_tag="ACG8FM_002615"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS5095211.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2815..3513)
                     /locus_tag="ACG8FM_002616"
     CDS             complement(2815..3513)
                     /locus_tag="ACG8FM_002616"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS5095212.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3525..4304)
                     /locus_tag="ACG8FM_002617"
     CDS             complement(3525..4304)
                     /locus_tag="ACG8FM_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS5095213.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4320..5264)
                     /locus_tag="ACG8FM_002618"
     CDS             complement(4320..5264)
                     /locus_tag="ACG8FM_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5095214.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5310..6089)
                     /locus_tag="ACG8FM_002619"
     CDS             complement(5310..6089)
                     /locus_tag="ACG8FM_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095215.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1625 a         1298 c         1125 g         2175 t
ORIGIN      
        1 ataattactt tctgaaactt taggtaatac tattttttcg tcacacatat atagtgcggg
       61 tgctagttca gagcaatctg ctccaatgat ttttccttcc atttcattat tgtttaatgc
      121 tgttttaaat gcattcacta gttcacaacg acgtccacaa cttaatatta aaatattcaa
      181 tttttaatct cctatctatt cgaagcattt gcaaaagtga caatgtcttt agccaattgt
      241 tcatcgtctt gcggcaggct cttgacaaaa tccatcactt tttgtattga gtaacctttg
      301 atgtttccta caaagatttt ctcataaact gcttcatcat ttctttcctt atccaacaat
      361 agttcttcat atagtttttc ccctggacgg atacctgttt caatgatttc gatttcgtct
      421 tcgctatacc cgctcaatcg gatcatgttt ttcgctaggt ctacaatctt cactggttca
      481 ctcatatcta gaataaaaat ttcgccacct tttgctaatg cgccagattg aataactaaa
      541 cgactagctt caggaatcgt cataaagtat cgagtcatac gaaagtctgt tacagtgatt
      601 ggaccacctt gagcaatttg ttctctgaat actggaatca cactaccgcg agaaccaagg
      661 acattcccaa atctgacagc tgagaatttc gtacagcctt cttcattcaa tccagtcaca
      721 atcatttcag cgattcgttt tgttgaaccc atgacatttg gaggattgtt agctttatct
      781 gtcgatacca tgacgaagtt tttcactttt gctgctttag ctgcttctgc tacatttttt
      841 gtaccgaaaa tattgttttt gactgcttct tttggattat attccatcag tggtacatgt
      901 ttatgagcag ctgcatggta gacgatatct ggatgatatt gttccatgat ctcattgatt
      961 ttctcgcgat cttggatatc tgcgatgatc ggtacgattt ctgtaggaca attttgatga
     1021 tgcgtgcgta actcacggtc aatcaagtag atcgagttct ccccatgtcc taaaagcaat
     1081 agctttgcag gattgaattg aatgatttgc cgacagattt ctgaaccgat cgatcctcct
     1141 gcgccggtga caagaatgac tttgtctgtg atctgatctt tgatggattc aatatccagc
     1201 tttacctcat ctcttcccag taaatccact acgtcaattg tttttaattt tgatacgcta
     1261 attttccctg aagcaagttc ttctatagaa ggcatcgtgt tgactttcac acgagcggat
     1321 tcgactaact cgaagattcg tcgtaatttt tttcttgata aggaagggat agcgatcgtt
     1381 accatttgaa tgtcatattt ttcaatcaat tccggaatat cttttaatgc acctaatact
     1441 ttttttcctg aaagatacgt atttctttta tttggatcat catctacaaa cccgacaacg
     1501 tggatatctt gagccgtttt ggatccaaga aagctgttgt ataaaatacg cccgccttcg
     1561 ccagcaccaa caatcaatgt atttttagca gaatctgctg atacgtatct catatttttt
     1621 gtttcgatgt agatacgcca gatcaatcgg ctaccgatga tcaataagag cgaaagcaga
     1681 taagcaaaga taactaaccg taaactgtat cgccgattaa tgaacagcag tatgaggata
     1741 cttgaggcag cacttgcaga tagtgaacta aaaattgcaa tgatctctct taaatttgta
     1801 tagcgattga tccgagtgaa aactttgaat aaactaccat aaaataaata aaaaccaatc
     1861 gacaaaccga tagagattaa aataagatcc attggtatag atacaaacgg cttcatgaat
     1921 ttagctgctg caatatttgc agcaattaat aacaagctgt ccacaaaaac caatgtggca
     1981 atttttcttc ttcttgttaa tataaacact ttttaattcc ccctaaaata aaccaaactt
     2041 cttctttttt acctcttcat agtctggata actgatttca tccccgttga tcagatcctt
     2101 tgcaacttgc ttcatctgta ctactttttc cttaccaaag tccttggcaa tctgctcata
     2161 gcactctttt aagtaaaagg tgcgcttaga gactccgtgt gcatcagaag cagtcatctg
     2221 cacgagatta tgtttgacca ttgttttagc tgttttctga atatctttgc caaatacccc
     2281 aacataactt ggcgcagtaa gttgggccaa acaccccata tctaggaacg gaagcaaatg
     2341 attcgggtct tcgcgaaaat gcgcgttgcg ttctggatgg acgatcactg gtgtctttcc
     2401 tagttctaac aactcaaaaa aaagccgttc tgcatataac ggcacttcca gtgtcggaaa
     2461 ttcgatcaga agatatgtat catctaaatc tgtaaataaa atttcatctc ggtggatgtc
     2521 ttcgatgagc gttccggtaa tacgtacttc ttgtccttcc aataacgtca aaggcaactg
     2581 tcttttctct aattcggctt gtaacgaagc gactagagaa atgacttgtg atttttcatt
     2641 gctgtatttt ccattattgt ggtgtggtgt gcataaaata tgcgtgatgc cttgctgaat
     2701 cgctttttca gccattgcaa tacttgcttc taagttttct gccccgtcat cgattcctgg
     2761 taggatatgg caatgtagat cgatcatttc tcttgtctcc tctcttctta ttccttaatt
     2821 tccgtagtaa taaccagcat ctttgctatg ttctgctccg ttgtatacaa cacctaaaac
     2881 tcgtgcttgt accatctcta gcagttcttt tgctttattc aatgattctt tacgtgatgt
     2941 attttcacgt acgaccaata aagtgccatc cgtttttgaa gacatgatct gtgcatctgt
     3001 tacggctact actggtggca tatcgaatat caccacgtca aataactgtc ttgcttcgtc
     3061 taggatctga tccattcttg gcgaacttaa caattctgat gggttgggtg gcttaggtcc
     3121 actaggcaaa atcgacaagt tctcgactgg cgttcgttgg attacatctg ctacacttcc
     3181 tgatgaacta agtgctgtac ttaatcccct ggcattgttc agctggaatg tcttatagac
     3241 gacgggttta cgtaaatctg catcgactag tagaactcgt tgaccggatt tagcaaaaac
     3301 gactgcaatg tttgctgcag tggtggattt tccttctcca ggacctgatg aagtcacgac
     3361 aatcgttttg atttgctgac cagctgcgga ggcaaactgg atattcgtcc ggatcgtacg
     3421 atattgttcg gcgattggtg aagatgggtt tgttaatgtg atcaaactga ctgcatgagt
     3481 ttgtgctgct ttttgtttct gtgttcgtgc catttttctt cctcctagac tcttgagcgg
     3541 cttctgcgtg atggggctgt tttatcgttt gtagcttttg gtcgtgcagg agctgttcct
     3601 tttttacgga taccagcgtt caattctttc gctgacattt ctggaactgt tcctaagatt
     3661 gggaatccta atgcatccgt cacatatttt tcatctttca ctgtccgatc aagtaattcg
     3721 aataagaatg ccagacctac gcctaccatc attcctaata ctaagccaat cactagattc
     3781 aatttattat ttggcgaaac aggtgtcgta ttggcaactg cacctgaaat aatcgagatt
     3841 ttatcgacat tcatcacgtc ttttgcgttc tcttggaaaa cttgtgaagt tgtgttagcg
     3901 atctgttgag cagtcactgc attcgtgctt attgcctgga tcgagaacat ttgtgaattt
     3961 tgtgactgat tgacggagat cgcatctttg atttgtccag cagtcatttt cacattgtct
     4021 tctgtttcta aacgatcttt gacttcattt agtactaaat cactgacgat catatcttta
     4081 tacgtattga tcatttgcag gttcgtattg acatcattga catttgctgt gtctgattgt
     4141 ggcagtgtca cgatcaattg tgcttgtgaa ctgtattttg gtgtcaagat gaaaaatgtt
     4201 acgattcctg agatggccaa gccagcaaac attgagatga tgattgttac caagtgtttg
     4261 cgtaatgttt caaaaatttc tttcaagctg attgtctctt ccattttttc tccactttcc
     4321 tatttattct ctagattcat ttgatctttt aattcttttt tcacacgatc aagttcttga
     4381 tcgctgactt tttgatagga gatattatct tgcatgaagc cttctccttg cagctgatct
     4441 tgcttgatcg taccaaatgc ctcccgataa tctaaagcga tttttttcat ttcatcaaag
     4501 ctcatgtctg ttttcatatt cgactctaga gcattcaaaa cggattgata gttgctgaca
     4561 ccgtttaaac tcaatacttt ttttgctaca ccttctacga tctttctttg acgctcttgt
     4621 ctaccatagt caccatttgg atcttcataa cgcatacgag aataagataa tgcctgatcc
     4681 ccgttcaatg taattttacc gattggaaag tcatagccat cttgtgagaa agtgaggtca
     4741 ttattgactt cgataccacc aactgcatca acgagttcct ttagcccttt catattgata
     4801 gagacatagt gatcgattgg gatgtctaca tatttttgta gggtatccat cgccatggca
     4861 gctccgccga atgcgtaagc gtggttgatc ttgtcttttg tgtcacgacc tacgatgtcg
     4921 acatacgtat ctcgaggaat gctgacgatc gttgtctgtt tgtcttcagg actgactgtg
     4981 gcaaccatca tcgtatctga tcttccttga tcgacccgac ctaaatctcc agtatcaatg
     5041 cccatcaata gaacagagaa agattcttgt ttgtccagat caactggttt cgttcgttct
     5101 ccttgggttc gttccactga ttcgtaagtt ttctcaatag aatgtgagag atccagatat
     5161 aattttgctc ctactcctac aattgctaat actaatacga taagaactgc taaaataatc
     5221 agaaatattt ttttccctct tgattttctt cttcgtcttg ccatagtttc ctccgtttgc
     5281 taaccttgct tgaatcaacg ttttaactgt caataactta tattgctata gcccatttgc
     5341 tgagcaacag tttgtgcttt tgttgcgtat gctgcgctta ttttttcatt ccggatctgc
     5401 tctatttgac tgagcgccgt caaatattga tcgtaagtag cggtggcagt cacttgacca
     5461 tccttcaaca tcgtatcgaa taactgatct agttgcgtga cttgcgcgat ctgagtatca
     5521 gcgaatccta gatattgcat aacaaccgtt ttccaaggac tatctttaaa aaagctcata
     5581 ttttcacgta cacgagcgac atctgcactt gtcacctttt ctttgatgac gacatcgtct
     5641 acagcttgct gccagtttga tacattttta gtaaataaat cgttgacagc tgtctggatc
     5701 ttcagttttg attctgcact atctaactgt gtcacgattg ctttcttctc atcttggatt
     5761 gcaacagctt ctttaggcaa ttctgattct ttgatgtcgt aatcatctgc tgaaagtttg
     5821 acagaggata gcttatttga caaacttgtg attgtttctt ctgttatttc agggtttaga
     5881 aaattttttt cttttgtact atacagcttg tctacttctt ttgataaaga agtcaaggtg
     5941 tctttttgat tggtgatggc agattctgca taattgatcc gttgctgatg ttgccgctga
     6001 ttattgatat ggtaaccaaa caaacagatc gcaatagcac ctgcacctac cgccacagca
     6061 gtcaatgact tgacgaatgg cttttccata gattcactcc taagtattga tgatagataa
     6121 attaagatat aattcacata cataaaaatg acagcttttt attttaaaaa acataattaa
     6181 attaatatat tcacagaaat atttgtaaac aagttttccc tat
//
LOCUS       DAZUMT010000131         6181 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000131 DAZUMT010000000
VERSION     DAZUMT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.135"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG8FM_002620"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG8FM_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS5095216.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACG8FM_002621"
     CDS             499..861
                     /locus_tag="ACG8FM_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5095217.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACG8FM_002622"
     CDS             913..2595
                     /locus_tag="ACG8FM_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS5095218.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACG8FM_002623"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACG8FM_002623"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS5095219.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACG8FM_002624"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACG8FM_002624"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS5095220.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACG8FM_002625"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACG8FM_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5095221.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2056 a         1078 c         1418 g         1629 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 a
//
LOCUS       DAZUMT010000132         6174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000132 DAZUMT010000000
VERSION     DAZUMT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.136"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8FM_002626"
     CDS             725..811
                     /locus_tag="ACG8FM_002626"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5095222.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1622)
                     /locus_tag="ACG8FM_002627"
                     /pseudo
     CDS             complement(932..1622)
                     /locus_tag="ACG8FM_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1629..1907)
                     /locus_tag="ACG8FM_002628"
     CDS             complement(1629..1907)
                     /locus_tag="ACG8FM_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095223.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1867..2781)
                     /locus_tag="ACG8FM_002629"
     CDS             complement(1867..2781)
                     /locus_tag="ACG8FM_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5095224.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2763..3143)
                     /locus_tag="ACG8FM_002630"
     CDS             complement(2763..3143)
                     /locus_tag="ACG8FM_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5095225.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3593..3880)
                     /locus_tag="ACG8FM_002631"
     CDS             complement(3593..3880)
                     /locus_tag="ACG8FM_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5095226.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3900..4124)
                     /locus_tag="ACG8FM_002632"
     CDS             complement(3900..4124)
                     /locus_tag="ACG8FM_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5095227.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4579..4749)
                     /locus_tag="ACG8FM_002633"
     CDS             complement(4579..4749)
                     /locus_tag="ACG8FM_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095228.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4764..5297)
                     /locus_tag="ACG8FM_002634"
     CDS             complement(4764..5297)
                     /locus_tag="ACG8FM_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5095229.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5318..>6174)
                     /locus_tag="ACG8FM_002635"
     CDS             complement(5318..>6174)
                     /locus_tag="ACG8FM_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5095230.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa atataacccc tggcaataaa
     1501 aacctcttaa atcgaatttt agagcctttt aatgaatatc ttgtttgata agccttatag
     1561 ctaagccaaa taattcctaa aatactggca aaaatgagta gatagggtaa atagaatgcc
     1621 atttctattt attctcccca taccagccaa aaggctaaca gtgtcatgat taagcaacat
     1681 aagggggcaa gaatatccat tgatcgctgc ataattactt ttttctgttc tttatttcgc
     1741 tggtttaata gcgcatggtg gaattttttg acttgtgaat caattgtagt ttgtattctt
     1801 tctttggtat aaggtgcaag aaaagcacgt tgtttcttta tttcattttc tatgtcttgt
     1861 aacgttttag aacgataatt cttgttggta gtctgctcct ctttcaacgt gttggtcatg
     1921 gtttcttcga gtcggtctaa tttccggttg ttcagtcgta agagttcgtt gttctcttcg
     1981 tacttctttt gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgttg
     2041 ttgattgtag gctcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtaatc
     2101 ctttcctagt ttgttggatc gtactttttt ctttttgtgg atcaattcgt aagtaaaatt
     2161 tttcccacga ttcaagcaat gaatgccgaa tgttttaaga gaatcacaaa attggttata
     2221 cgttcgagtt tttttgtttt tcattgtttg atcaatcgca aaccgaattt cgtctttcca
     2281 aacaggttca tttttttctt ttaatttgat ttcagacatg gttcgttttt cgggtttctt
     2341 ccctcgttca gtgattgtta agtcatgttc aagacaaatc tcgtcattta ggtcataaca
     2401 tttgtttaaa aatgcttgac catgggcttg aaatttgttc ccagtttcca gattgacgga
     2461 gttaatgaca atgtggttat gaatatggtc tttgtctgta tgtgtgtaaa tcgcgatttg
     2521 gtggtcaggg gctatttttt tcgcatactc tgcccctagt tgattggctt tttcagcggt
     2581 aatttctcct ggtcgaaaag actggatcaa taagtgtgct tgtacacgat cgtcttttcc
     2641 atacagcatg cgaatttgtt tcatttcgct tttggcataa tcaatgtcac agtttatccc
     2701 tgttttgacg gttgctcgtg gttcggcgta attgatcgct cggctacaag aagtggttcg
     2761 gcttacttta actgttgcca tatcttttgt agctcctttt gcatgatttt taggttttcg
     2821 gataagcgtt gggcgtcgcc aggatcaacg tcgtgttggt gtgtgttgca ccacttggca
     2881 atttgattga tattcccgcc agctttggct aaatcgcgcg cgattgcctg agcaatgtct
     2941 ggcgccactt tcggtgtcac cagacgcgcg ttttgtgcct tggctttcac gaaagccggc
     3001 acactcattt gcaaagcttc agctgactgc ttcagctttg caaattcgga ttcgctcact
     3061 cgaaaactga tttgttttgg ctcggatcga taggtatatt ttttagaatg attgctagcc
     3121 aaatattgat tttgttcgct cataagtccc accctttcag tacgtcctac ggacgtatcg
     3181 ttcgcaatac cattgtacct aaaaaggtac aataagtaaa gctcactaaa gctcactaaa
     3241 gggcttggca agctctatat ttgctagcca aatacctacg tatttggtgc aaataaccgc
     3301 ttgtcaaagg ggcgcaagct cccccttgaa tccccctccc ccccactctc cacaatacaa
     3361 aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac cttcggtgaa
     3421 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     3481 gccatttact attaataaat ctataaatat gataattttt cggctctata atttatggga
     3541 ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag aattaactcc
     3601 aaataccaat agaagcccat cctctatata cttgagattc cttactaaca atttcttgta
     3661 attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt ccaagaaaac
     3721 tacttaattt catactaact tcaccaacac tttttccctc actgatttct ttagaataaa
     3781 gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg tatactttac
     3841 taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc tccctaccac
     3901 taccttctag gtgcccatgg accatgatta acccaaccat taacaataat ttttccaatt
     3961 tctcctttag cttgattcca atctacccaa catttttctt tgttacaata aagaccattt
     4021 ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag acaagttcct
     4081 aatattccta gaataacaca atgttttaat actttctttt tcatgattgt ttttcctttt
     4141 cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta tataacattt
     4201 tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata
     4261 aaattataag atcagttatt taaacagcta gtagacataa tgtgtatact accaagtaac
     4321 gcctgtacca agggatccag gcgctgattt tgtgaatttt tattggtcta ggttaaaagc
     4381 tctttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg
     4441 gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat
     4501 attcatttat tattcttttc taaggttaat aataggataa tctctttcag aaagactgct
     4561 cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact
     4621 gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga
     4681 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     4741 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     4801 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     4861 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     4921 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     4981 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     5041 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     5101 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     5161 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     5221 tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag
     5281 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
     5341 cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag
     5401 cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     5461 gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta
     5521 aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt
     5581 ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc
     5641 cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt
     5701 ttataactcg tttatctata tcaggttgct tataactttt aggaatatct aataattcaa
     5761 gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa
     5821 acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc
     5881 actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag
     5941 gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa
     6001 gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta
     6061 aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt
     6121 caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg atgg
//
LOCUS       DAZUMT010000133         6154 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000133 DAZUMT010000000
VERSION     DAZUMT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.137"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..1099)
                     /locus_tag="ACG8FM_002636"
     CDS             complement(137..1099)
                     /locus_tag="ACG8FM_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5095231.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="ACG8FM_002637"
     CDS             complement(1101..2540)
                     /locus_tag="ACG8FM_002637"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS5095232.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="ACG8FM_002638"
     CDS             2725..4671
                     /locus_tag="ACG8FM_002638"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS5095233.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4935..5807
                     /gene="scrK"
                     /locus_tag="ACG8FM_002639"
     CDS             4935..5807
                     /gene="scrK"
                     /locus_tag="ACG8FM_002639"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5095234.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1958 a         1051 c         1093 g         2052 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca atgaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2881 acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4741 agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta ttagaatttt
     4801 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
     4861 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
     4921 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
     4981 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
     5041 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
     5101 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
     5161 ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag
     5221 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
     5281 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
     5341 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
     5401 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
     5461 actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg aaaaaaggac
     5521 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
     5581 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
     5641 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
     5701 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
     5761 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     5821 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     5881 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataaatt
     5941 cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt
     6001 tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat aggttctgtt
     6061 gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata gcaggaggaa
     6121 tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DAZUMT010000134         6068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000134 DAZUMT010000000
VERSION     DAZUMT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.138"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG8FM_002640"
     CDS             832..1566
                     /locus_tag="ACG8FM_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5095235.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG8FM_002641"
     CDS             1582..2751
                     /locus_tag="ACG8FM_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5095236.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8FM_002642"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8FM_002642"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5095237.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG8FM_002643"
     CDS             3841..4767
                     /locus_tag="ACG8FM_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5095238.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG8FM_002644"
     CDS             4786..6036
                     /locus_tag="ACG8FM_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5095239.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAZUMT010000135         5910 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000135 DAZUMT010000000
VERSION     DAZUMT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.139"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(525..1028)
                     /locus_tag="ACG8FM_002645"
     CDS             complement(525..1028)
                     /locus_tag="ACG8FM_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5095240.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1387..2412
                     /locus_tag="ACG8FM_002646"
     CDS             1387..2412
                     /locus_tag="ACG8FM_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5095241.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2735..3613
                     /locus_tag="ACG8FM_002647"
     CDS             2735..3613
                     /locus_tag="ACG8FM_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS5095242.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4089..4205
                     /locus_tag="ACG8FM_002648"
     CDS             4089..4205
                     /locus_tag="ACG8FM_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5095243.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5133..5471
                     /locus_tag="ACG8FM_002649"
     CDS             5133..5471
                     /locus_tag="ACG8FM_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095244.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5565..5795
                     /locus_tag="ACG8FM_002650"
     CDS             5565..5795
                     /locus_tag="ACG8FM_002650"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS5095245.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2009 a          888 c         1081 g         1932 t
ORIGIN      
        1 aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1321 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1381 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1441 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1501 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1561 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1621 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1681 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1741 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1801 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1861 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1921 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1981 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2041 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2101 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2161 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2221 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2281 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2341 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2401 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2461 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2521 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2581 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2641 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2701 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2761 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2821 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     2881 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     2941 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3001 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3061 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3121 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3181 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3241 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3301 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3361 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3421 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3481 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3541 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3601 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3661 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3721 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3781 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3841 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     3901 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     3961 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4021 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4081 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4141 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4201 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4261 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4321 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4381 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4441 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4501 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4561 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4621 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4681 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4741 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4801 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4861 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     4921 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     4981 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5041 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5101 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5161 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5221 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5281 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5341 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5401 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5461 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5521 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5581 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5641 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5701 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5761 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
     5821 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact aactttaatg
     5881 cagtataaag agtatccaga gtattttatt
//
LOCUS       DAZUMT010000136         5837 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000136 DAZUMT010000000
VERSION     DAZUMT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.140"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1367)
                     /locus_tag="ACG8FM_002651"
     CDS             complement(69..1367)
                     /locus_tag="ACG8FM_002651"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5095246.1"
                     /translation="MHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYP
                     SKDTAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLS
                     LPFYNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQ
                     FVTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQ
                     KETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHF
                     IKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKL
                     ISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQ
                     PKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(1495..2010)
                     /locus_tag="ACG8FM_002652"
     CDS             complement(1495..2010)
                     /locus_tag="ACG8FM_002652"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS5095247.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(2083..2997)
                     /locus_tag="ACG8FM_002653"
                     /pseudo
     CDS             complement(2083..2997)
                     /locus_tag="ACG8FM_002653"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(2994..5321)
                     /locus_tag="ACG8FM_002654"
     CDS             complement(2994..5321)
                     /locus_tag="ACG8FM_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS5095248.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         1529 a         1120 c         1099 g         2089 t
ORIGIN      
        1 tgtcacaacc tttctcccca tttgaatcac tatcgtttat ttatcgtatg atttttgttt
       61 ccaatagttt aagcttctgt ttcttgatgg cgttttttga agtagatcac tgctcctcca
      121 actaaagcag tacctaccaa gatgaatacg atgatcccca taccaccagt tgaaggcaag
      181 attcctttag gttggttatc gatcgttgtt tcatcagaag ttgttggatc atcatctact
      241 gtaaattcag tgtacgcagc ttcattctta ggtaatgcgt agccttctgg tgcttttgtt
      301 tcttgaaggc agtatgcccc ttgtttcagc ccttcgattg aaaatttccc ttctgaatca
      361 gaaatcaatt tcacttcttc ccagtcagaa cctgtgactt catctgacca agcatatggg
      421 tattctcctg aggcaagatt tgctattttt ttcattaatt taacagttgg atcttttgct
      481 cttagaatag aaaattctgc atcggctaat ttttcttgcg tgttcacatc ttgtttgatg
      541 aagtggaaat caccgtagtt ttggacattt tctacagtaa tcgtgttatc cgtagttgta
      601 ttttcccgat tgactacacc gtttggattc ccaagattgg cttcatctgc gccatggatg
      661 ataccggttg tttgttgcaa ttttccgttt tttactataa aagtagtttc tttttgtgga
      721 ttggttaatt gataaccatc tggtgctttt gtttcaacta aagtataatt gccgtctatt
      781 aatccattga cactgatgga tccttcagta tcagttgtga atgtcaacgc tgactcagca
      841 ggatcataga gagcgacacc agtgttttca tcaaaaccgg tcacgaattg attctctgaa
      901 tttttgatct ggaattgcgc ccctgctaac ttatctaccc ctgaaaagtt cccagctttc
      961 tggattttga tattgctttc tttgatgaca tttttagcat ataggtgaat cgtgtctaaa
     1021 ggtttgtcag ggtctgatga attcgggtta taaaagggta agcttaacac gatatttggc
     1081 gacgtttcga caccgggctt tggtgtttct acaaagacgt atacagcatc tttcccatca
     1141 gatttacctg gaagagtcag gttagctaat ccttcaccag aagtcgttac acttgcgaag
     1201 ggtgtagcgt ctgctggaac tgctgtgtct ttacttggat agtacgtagc agtgatgtca
     1261 tatacgttga attcaacgcc gttcaacggt tcgctattcg gaaatgtcaa ttccgttccg
     1321 ttgtttattt gattttctgg tgctgaagtg aatttcaatt tatgcaaatt aaccgtaaca
     1381 ttttctggtg taggtgatac ggcctagaaa cctctgcagt tcctaggccg agaacgaaag
     1441 gaagcagcat gatagcagtt cctaaaaacg tccatatttt tgttttgatg tttttcattt
     1501 tatttccctc ctattgtttc ctttttgttg ttttttataa aaccaagctg tccctgttga
     1561 aaggactagc aacaacccta aaattgagaa acctccaagc atctcttcgc ttgtttttgg
     1621 aaagcttcct ccattttttt catttgatgg accatttgtc tgcatctgaa ctggcgtttt
     1681 tccattattc gtcggatttg cttttggttc gttgatgatt tccaatggta taggatgaag
     1741 cggatcataa ctgccttttt caacttcaaa gagaatggtt gattgactca atatatatcc
     1801 tttaggcggt ttaatctcca ctagttcgta acttccaaaa gacaatccat ggacagaaaa
     1861 tgttccatct ttactggaag taaaaatcgt tgctcttgaa tcatctttgg ctacccaatg
     1921 atttatcccg ttttcttgcg ctaagtattg gtcatttttt ttgactagaa attgagcatc
     1981 tgctagtttt tgtttcgttt gccgatccac tttgacaaat tgtttgcctc ctgtttggat
     2041 ggcaagatgt gtttctactg tttgattgtc agtcgtcaaa cgtacatcgt tatcaaatgt
     2101 cgttttttca ctgttttctt tcaatgtcat ttggtaacgg atcgttaatt ttttcccagc
     2161 aaacgaacta agatcttttc cagaatctga taagacaaaa ccatgttttg ttttttgtgt
     2221 gtgaaatgta tgaatcttgt ctccaccaat caaaaaatcg atcgtttctg gttctagcca
     2281 taactgagga tcagctgtat ctttgatcgt ataggttcct atctcgttga tgttctcagg
     2341 aattgttacg gtgatgagat aaggaacttt atccccatct gcaaaatctg tctgcttatt
     2401 ctctactgtt tttgttaatg tgagtgcagc aggtattttt tgttcgctct ttggatacaa
     2461 gtggatggtc gtcaatttat gattagatga atctgtcaca ggaagcacaa cgacaaatgg
     2521 tgctgatttt tcttttactt aatctgaagt ctttatttcc gcaaatcggt aaacagcgtc
     2581 acgaccttgc tcatctttgt ccgatgcact aaaagaagca atgccctctt cctcgtctac
     2641 tgtttttgtc acattttctg ccagtatttt ttcactatca cttttttgtg ctagttttct
     2701 ttgtgcgtct tccacactac ttccttctga ccgcagcttg taaaattcat ccgagacatc
     2761 atatatagcg aaagtaacgc cgttcaaccc ttttactcct tgaaagttat ccgggatatt
     2821 tattccgtcg ttttgactga tacttggcat cttatcgctt tcgaacattc gattatgcaa
     2881 agtaaatgta ataggtgatt ctctagctga cacagtcgga gaacctataa aaaagaccag
     2941 taggataaaa aagagaacag caaatatatc cacacgtaca ttttttgttt ttttcatcgc
     3001 tgttcacctg cttttttctt acgatggata aaataaaaga agccagaaaa aataaagatc
     3061 agtcctaaaa agacaaaggg cagtagacct atcccacctg tcgaaggaag caagagagtc
     3121 ggattgatcg tcaacttagc ggtatctgtt gcgataatcg tataattatt gtttacactt
     3181 ccttcgccta aactaatcgt tactatttgt gatccaatat ctgtccggct gttaactgtt
     3241 agggtataat ccaatgattc gcctaagaca acaccggtaa cgtggagatt tccaaccgaa
     3301 atcgagtgac tttgggcatc ataattaaag gcagcgtcat cgacagtaat cactgcgtta
     3361 gctttataga tggtataagt tcctgatgtg ttggctatat cagtaaatga caacttggga
     3421 tttgctgctt tgactttgtc tattccgcta ttcgttagtt taattgtata gctgccagca
     3481 ttaaccatat tttttgctga ttgtgcatct gctgtgtaat ccatactgct aagtgccact
     3541 tgtactggag taagcgaact agtgacaccc tctgctagct tgacatggat tgacgggaca
     3601 cctgcttttc catcatatgt cttactgcca ctgagctcta ccgcaccgct gatttgctta
     3661 atcgtataga catcagatgt ctgcacgata gtaaataaat agtttgttct ggcagctagt
     3721 ttagctagtc cgctttttgt caattgccaa gacgtatagg ttccaatttc agtaggcgca
     3781 gttacttcaa ctcccttttc attcatcgct ttgaaatcag ttggattcca tgtgatttga
     3841 tcaatactga gatcattgtt taaaagggat gttgattctg ttttatcatt aaaggcatat
     3901 ttaacgatcg gtacaacaga aagaccatca tagtacttac tgccatttgc attgattgat
     3961 actgttgcat ttgtcttata atataggatg acttcagaaa tctgttttcc atttgcgtcc
     4021 tttacgatta catttccatt tgtatctaaa ctagctaaaa cactcattgc tgattggttc
     4081 tttgtttctg tcgctgaaac agctgtaaag gtatatccgt tgatagtagg cgtattagtc
     4141 gtcaggtctt gtgcagcact tccgtaagta agattcatta ttttagaagg agcaatcatc
     4201 tgtccaaaat agtcttgata cttaatagtg attggtgttt gtttgcctgt ataggtcacc
     4261 gtatcgacgt tactttctag ggctgtgaaa gaaccgtttg tttgtgcaaa agaatagccc
     4321 gttactgctg ttggagtgat aaccactttg tcacccacat ggattccaga taatgtagtc
     4381 ccgtacgttc cgatttttgc tgctccacca ttgactgtat aagaaaaagt tcctgtccga
     4441 ttcgcattga cctgaaccgt tgctgcttga taggttggaa aatttgaaac atttccctta
     4501 ccatcaacga tctgcccatt tttgatcgag taccccgtga atggaatatc atttgctaac
     4561 tgtataccga ttgaaaaagt actcatacct cctccggcag gaagccctcc ccatgttgag
     4621 ggtgtaaaag aataactttt atccgcattg ggtgtaactg aatcgatcgt atttccagcg
     4681 gtagccaccc ccatatagat actttttggg aatgtgatag tagtaccatt tactgtggag
     4741 ccaataaaca gattatggat atctggacgg ttatcaataa aagcgattgc tttggttacg
     4801 ccttgtaacg cagttttatc cgcactcatc gatccgttaa ccgcaacaat gactccatct
     4861 ttggcatata tcactgtata tccatctggt atggtatgtc cgttgaaatt cgcagtggcc
     4921 gcaacagtac taaatgtctg gcttctacct gtttctgaat aagctttctc ccctgctgct
     4981 tttgctgctg tgatttcttt ttctgtcgga tcaatcagtt gagtcgagga tgtggttgag
     5041 gttgtctcgt tgttgctaga ctgattggtg ctttctatgg actgtatttc agaatagggt
     5101 ggactatttg tctcctctgt cgtttggtta gcaaaatttg acgtttctgt ataaacgttt
     5161 acataaatac ttgataagct ccctaagaaa gtaaaaatca agatgatcga aatcataatg
     5221 ggatgtaagt attttctttt agatacagct ttgagtctag aaacaaaatg tttttgacat
     5281 atacgttgga ttttattttg agttttctta atcattttca tccctcctct caataaaaaa
     5341 tcatcaaata atcctttaca cgttattcta aatttaattt ttattatttt attgatgtat
     5401 agaaagtttg gggctttttt tctcttttta ttctaaggtt attttagtct tgtcctttaa
     5461 tacaaagatt ttatttttaa ataaaaaata ctcttttttt acgattattg aaaaaaaaag
     5521 ataaaacttg attaattgaa gtattgctta aatttggaag ctatgaggga agaataaaag
     5581 agttaggacg ggattatcta ctatttttct ttgttttaaa tatttgttat ctttgaacat
     5641 aaaagagaaa tgtgtcgact gcagtgttgt gtccctcatc acgaataaac ggtgttcaat
     5701 tagctgatct tgtattcact taaaaaatgg taaagtacga gttgctattc cccattttca
     5761 attttctaac gctcaggaca aaatactgtt ttcacaacct cattggaata aaagcatata
     5821 tattgtttca agaacga
//
LOCUS       DAZUMT010000137         5808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000137 DAZUMT010000000
VERSION     DAZUMT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.141"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACG8FM_002655"
     CDS             complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACG8FM_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS5095249.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2240..4426)
                     /locus_tag="ACG8FM_002656"
     CDS             complement(2240..4426)
                     /locus_tag="ACG8FM_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5095250.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACG8FM_002657"
     CDS             complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACG8FM_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS5095251.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4648..5088)
                     /locus_tag="ACG8FM_002658"
     CDS             complement(4648..5088)
                     /locus_tag="ACG8FM_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS5095252.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5162..5701)
                     /locus_tag="ACG8FM_002659"
     CDS             complement(5162..5701)
                     /locus_tag="ACG8FM_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5095253.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1503 a         1305 c         1015 g         1985 t
ORIGIN      
        1 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
       61 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
      121 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
      181 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
      241 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
      301 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
      361 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
      421 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
      481 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
      541 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
      601 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
      661 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
      721 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
      781 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
      841 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
      901 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
      961 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     1021 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     1081 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     1141 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     1201 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     1261 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     1321 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     1381 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     1441 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     1501 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     1561 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
     1621 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
     1681 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
     1741 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
     1801 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
     1861 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
     1921 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
     1981 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
     2041 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
     2101 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
     2161 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
     2221 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
     2281 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
     2341 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
     2401 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
     2461 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
     2521 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
     2581 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
     2641 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
     2701 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
     2761 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
     2821 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
     2881 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
     2941 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
     3001 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
     3061 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
     3121 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
     3181 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
     3241 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
     3301 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
     3361 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
     3421 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
     3481 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
     3541 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
     3601 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
     3661 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
     3721 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
     3781 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
     3841 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
     3901 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
     3961 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
     4021 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
     4081 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
     4141 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
     4201 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
     4261 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
     4321 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
     4381 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
     4441 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
     4501 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
     4561 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
     4621 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
     4681 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
     4741 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
     4801 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
     4861 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
     4921 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
     4981 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
     5041 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
     5101 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
     5161 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
     5221 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
     5281 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
     5341 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
     5401 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
     5461 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
     5521 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
     5581 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
     5641 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
     5701 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
     5761 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaa
//
LOCUS       DAZUMT010000138         5780 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000138 DAZUMT010000000
VERSION     DAZUMT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.142"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1282
                     /locus_tag="ACG8FM_002660"
     CDS             290..1282
                     /locus_tag="ACG8FM_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5095254.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1342..2763
                     /locus_tag="ACG8FM_002661"
     CDS             1342..2763
                     /locus_tag="ACG8FM_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS5095255.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2756..4255
                     /locus_tag="ACG8FM_002662"
     CDS             2756..4255
                     /locus_tag="ACG8FM_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS5095256.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4294..5688
                     /gene="uxaC"
                     /locus_tag="ACG8FM_002663"
     CDS             4294..5688
                     /gene="uxaC"
                     /locus_tag="ACG8FM_002663"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5095257.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1901 a         1068 c         1183 g         1628 t
ORIGIN      
        1 tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga
       61 aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa cacaaaatat tatagaaccc
      121 taattaaatg agcattcccc gctttcaaga atagcataat taaaaataaa aatccaagta
      181 atttttaacg tgtgcaatct tttcatatca aagatctatt gacttatcgt agttatcgta
      241 atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa aatgatggaa tggtaacgat
      301 caaagatatc gctaaagcag ctggcgtttc tcatacgaca gtttctcgtg ctttgaataa
      361 cagcagttta ataaaaaatg aaacaaaaca aaaaatacga caactagcta aggaaatgaa
      421 ttattcgcca aactatagtg caaaaggttt agtgaatcaa aaaaaatatc taatcggact
      481 atttttctca agtttgcata aaggaacttc atcaaatttc ctagcagatt ccattgcagg
      541 catccattca gtattagaca caaattacag tttatctgtt gaaagtatcg atgttgttga
      601 tttaacagaa atcaacctgc aacgctacga tggactgatc gttatgagtc aaagtgatga
      661 tgatcagcct ttcatcgact atctcaaaga aaaagaattt cctttcgttg ttgtcaatcg
      721 tcacatcaat gatccagcgg ttatcaacgt cactgccgat gatgcggccg gtgttatgca
      781 ggcagtagat tacggtattg gatgcggaca tacgaagatc gcttatattg gcggaaagaa
      841 aaattttcgt tcgacaaaag aacgggaaaa aggggtattg aaaagtctaa aaaaagcgag
      901 catccctgtc aatctagcct attttttaac aggagattac agcttagaaa gcggcctcca
      961 aaacatggaa tacctattat cactttctga cctgcctact ctggtttttt gcggcaacga
     1021 cgacatagcg attggtgcat tgagagctgt acatttagct ggcctccgtg ttccccagaa
     1081 catttctctg atcgggttcg atgattctcc agtcgtctcc tatctcaatc cgccgcttac
     1141 caccgtacgt aagcctttga aagaaatgtg tcaagaagga acaaagcttt tactagattt
     1201 gatcgaaggc aggacgatcg aacaatctaa aattgagctc tccaccttgt tgatccttcg
     1261 tgagtcgatc aattacttgt aaactacaag taattttata taaaaatgtt aacgtgtgca
     1321 taacacagaa aggaagttta tatgaaaaaa ctgacaaaag aattagtgaa aaaaaacgaa
     1381 gcacctgtaa aagttcttca gtttggtgaa ggcaatttta tgcgtggatt tattgattgg
     1441 cagattcagc agctgaataa ccaacaattg tttaaaggta atgttgcaat cattcaacct
     1501 ttagaacaag gtcttggaaa tatgatgaac cagcaagata atttatatac ggtcatttta
     1561 caaggactca gcaatggcga aatcgttgat acttccgaga ttatcaccgt agtagaacaa
     1621 atgatcaatc cttatgatga atgggatgct tatctatctc tagcagaaaa tgacgacttg
     1681 gcctatatcg tctccaatac aacagaggca ggaatccaat tcgtggagac tgataattta
     1741 acagatactc ccccacagag cttcccgggc aaactgacag cctttttata ccgacgtttc
     1801 aaattaaaca aagctggctt taccatcatt ccttgcgaac tgattgacag aaacggcgaa
     1861 cgtctaaaag aaatcatttt acgctacgca gaaaaatggg atttagaacc tgcttttgtt
     1921 gagtggttag aaaaagaaaa tgttttctgt tgtagtttag ttgatcgcat cgtcccaggt
     1981 tatcctcgcg aagatgcagg tgttttcaat caacggcata actatgaaga tcaattgatg
     2041 gtcaaagcag aaccattcat gctttgggtg atcgaagggc ctaaagaatt ggagaatcaa
     2101 ctaccattca aacaggctgg cttgaatgtc atcatcacag atgatatgac tccttatcgt
     2161 gaacgtaagg tgcatttgct taatggaccg catacagcga tggtccctct tgctttgttg
     2221 gcggaattgg aaactgtaga agaagtaatg aaagatcctt tattctccgc atatgttgat
     2281 cagttattca atctagaatt gattcctatg ctttctttac ctaaagatga attagccatt
     2341 tatgctgacc aaatcaaaga acgtttcttg aatccttttg cccatcacaa attagaagcg
     2401 atttcattaa atagtgtttc aaagttcagc acccgtctgc ttccagtgtt caagaagtat
     2461 atcgaagaac aaaatcaggt accaccgtta atcactgttt cactagctgc gctacttctt
     2521 atgtatcgag gcgatcaagt aaagccacat gatgatgaaa agacgataag cgaatttact
     2581 gatgcttggt ctgataatgg aacagctatt cctcgattat tgcaaaacgc tgcgctttgg
     2641 ggagaagatt tgtctcaaat accgaatgtg acagatacag ttcaagaaat agctgcaaag
     2701 atcgatcaaa aaggtgtccg gtcagtatta caagaaatga aaggaacgat ggtccatgct
     2761 tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac agtgtggtcg tcgcattaac
     2821 ccctattcca aaacaaacac aactgacgat tgatcagcaa acgatcacca ctcttgaaga
     2881 tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa gctggagaca acgtgatcaa
     2941 atatggctat ccaatcggtc atgtgacgac agacgtaact gccggtcaat ggctgcacac
     3001 tcataatgta aaaaccaatc tttctggtga attggattat cgatatgaaa aagatgtcca
     3061 tccaagtcat tacctttttg agaaccgaac atttcaaggt tacttgcgaa aaaatggaaa
     3121 agtagggatt cgtaatgatt tgtttttagt acctaccgtt gggtgtgtga atggcattgc
     3181 tgaactgatc gtcaaacagt tcaaggaaaa acatccagat ctgggacagt ttgaccatat
     3241 tacgatttta aagcatcctt atggctgttc gcagcttgga aaagatcatc aaaatactcg
     3301 agaaatttta gcggacgcag taaatcatcc caatgccgga ggagttttag tatttggatt
     3361 aggttgtgag aacaatacag ttcctgaatt caaaaaaatt ttaggtgaat atgatgaaga
     3421 acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag atcgaacaag gtgtggctct
     3481 attagaagag cttttaacag cggcggaaaa tgaccagcgc atatcagtcc cgctctctaa
     3541 gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta tcgggaataa ctgctaatcc
     3601 tttacttggt gcattttctg actacttgat tgctcaagga ggcagcacga tcttaacaga
     3661 agtgccagaa atgtttggcg cagaacaagt attgatggca cgagcggaaa atgaagaagt
     3721 ttttgactca atcgttgact tgatcaatga cttcaaacag tatttcttgt cttacgggga
     3781 accagtttac gaaaacccat ctcctggtaa taaagcaggt gggatcacta cattagaaga
     3841 taagtcttta ggctgcacgc aaaaatcagg aagctctcct gtcgttgatg tgctacaata
     3901 tggcgaaaaa atcagaaaac cagggcttag tttattgcaa gctcccggga atgatcttgt
     3961 agctgcctct gctcttgctt catccgattg ccagttggtc ttgtttacca caggaagagg
     4021 gactccgttt ggctcatatg tcccaacggt caaggtatct acgaatacaa ctctttttga
     4081 acgcaaaggt cattggatgg attttaatgc aggcgtctta ttagaacaac caatggaagt
     4141 aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct agtggagaag aaacgaaaaa
     4201 tgaacaaaat gacgttcgag aaattgccat ttttaaaaac ggagtaacac tttgattttc
     4261 ataaaacagt ataaaaagaa agaaggaaga tttatgtttt tatctgatga ttttttatta
     4321 aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa aaaacatgcc cattattgat
     4381 tatcactgtc acttagatcc aaaagaaatt tacgaaaaca acaatttcag caatctgaca
     4441 gaagcttggt taggaggcga tcattacaaa tggcgcttga tgcgagcttg cggcgtgcct
     4501 gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat ttttagcttg gtgtcaaacc
     4561 gtaccaaaaa ttatcggaaa tccgctttac agttggtcac atcttgaatt gaagcgtttc
     4621 ttcggtattg acttgttgat aaatgaagaa aatgcagaga aaatttggac acaagcaaat
     4681 gaaattttgg cagctcctga ttttcgccgt cgggaaatcg caaaaaaatc gaatgtagaa
     4741 gtgatttgta caacagatga tcccgcagac gacttgcact atcatcagtt gcttgctcat
     4801 gaagaaccag atttgaaggt tctaccttca ttccgtccag ataaagcact caatattgaa
     4861 aaagaaggat ttaatgcatg ggtcacacta ctggaagaag cagctgacca aacgatcacc
     4921 acctatcaag aactgataga agtcttaggg cagcggatag aatacttcca tcaacatgga
     4981 tgtcgtgttt ccgaccacgc tttagacatc ttacgctata aagaagcgga tgaaacagta
     5041 ttagaagaga tttttcaaaa agcacgagct aacaaactat tatcttcaga tgaaatcgat
     5101 gcttatctta cagaaacact gatacatttg acccacttct atcattctca taattggacg
     5161 atgcagctgc acatccatgc ttatcgtaac tgtaatacac aaatgttgga aaaattaggg
     5221 cccgataccg gctatgatgg aatgaatgat ctttctttga ctattcctct gcaaaaatta
     5281 ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga tcctttattc attgaatccg
     5341 aatgactatc ctgcgatttt agctttgatg ggctgtttcc aaaaagaaca gaatggaaaa
     5401 ctgcagttag gttctggatg gtggtacaac gatactcgag ctggtatgcg cgatcaattg
     5461 acacaatttg ctgatggtag tgctttgggg aattttgtcg gcatgctgac agattcaaga
     5521 agtttcctgt cctacactag acatgaatat ttccgtcgtg ttctttgtga atttttaggc
     5581 gaaatggtca tgcgaggaga agcacctgaa gatgaacaac ttttaggaag tctagttcaa
     5641 gcgattagtt atacaaatgc taaaaattac tttggctttt ttgcctaatt gattaatagt
     5701 aacagtaacg aatcaaaaac aggatagtga aataacggaa tccgtttatc cacgttttag
     5761 agaagaagac ggaccaaatt
//
LOCUS       DAZUMT010000139         5612 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000139 DAZUMT010000000
VERSION     DAZUMT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.143"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..400
                     /locus_tag="ACG8FM_002664"
     CDS             <1..400
                     /locus_tag="ACG8FM_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFS5095258.1"
                     /translation="NNIEEIIEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTE
                     SAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNS
                     HQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            681..1901
                     /locus_tag="ACG8FM_002665"
     CDS             681..1901
                     /locus_tag="ACG8FM_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5095259.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1898..4618
                     /locus_tag="ACG8FM_002666"
     CDS             1898..4618
                     /locus_tag="ACG8FM_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5095260.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4602..5546
                     /locus_tag="ACG8FM_002667"
     CDS             4602..5546
                     /locus_tag="ACG8FM_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5095261.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2087 a          899 c         1105 g         1521 t
ORIGIN      
        1 taataatatt gaggaaatta tagaaaagga tgctatcagc atttacaaat tttttatttt
       61 tattcctaat cgtcaattga aagctaatat atttaaccat ttgaaaaaga tgctaaatgt
      121 gtctgttact gaatcggcac ctgtaaatat tgaaatcgta cctcaaaatg tatctaaagg
      181 tgtggtgttt gatcgtttgg aatcaatcta caatttaaag aaacctttga gaatcgcgat
      241 cggagatagt ttaaatgact tatctatgtt tgaaagtgca gatattagtt ttgcaatggg
      301 aaatagtcat caagaaatca tgaaaaaagc tgattatgtc actgtttcaa atgataaaga
      361 cggagtatct caagcatttg aaagaattct taccatataa actaattaag gtagtataga
      421 acatagaatt ctaaatacgg aaaaatggag cataaattcc agtacaaata gtacttcttc
      481 agtgtcaaac gaagcgaata taacatagat atttaggatt gtgacaaaaa tcatgtcaca
      541 atcttttttt cgaataaact tattttttga agtcggccat ttatcgtaat ttctttgcat
      601 aatacgaaca aattttcggt tattgatgaa aaaaaacatt gaaaatggta tgataaaaga
      661 aaaaaaggag gaaattttta atggtgaaat ttatccatgc ggcagattta catatggatc
      721 ggtcatttga aggactgaca actcttgata aaactgtaca ggaaaaacta ttgaagacga
      781 atctgaccgt tttatcaaat atcattgaac aggcaataat aaataacgtt gattttgttt
      841 tacttgcagg agataatttt catcaaaatc gtccttcatt aaaaatacag aaacattttt
      901 cagaacagat gaaacggtta gaaaagaacc atatacctgt atttataata ttcggaaatc
      961 atgatttcta tcaaaaagag cggtattggt tctcctttcc taaaaatgtc catttattta
     1021 catccgaaac ggtagagaca aaaaaaataa cgataaaaag tggagaaact gtctctctca
     1081 gtggatttag ttatcgtcaa ccgtggattc aaaaagacaa agtgatggaa tttccttcta
     1141 gagaactcac agattaccat attgggttgt accacgggga acctggcgtc tctcaaaaag
     1201 gaaactatgc tcctttccaa ccctcaaaga tgcaagaaaa gggatacgat tattgggctt
     1261 tagggcatat ccatgtgcca acggtcttaa atgaaaagca aacaattgtt tatccaggtg
     1321 cgcctcaagg tcatactaaa aaggaacaag cttctacatc tattctacta gttgaattaa
     1381 gtaaaggaag ttgccaaata catccgatta aagtagcaga ggtctattgg aagacaaagg
     1441 aaatatcatt gaggaaagca agaacgacaa aagaggtcat tgttcacatt cgtcagcagc
     1501 tttcaaaagt agaggatgga tttagtttaa ttgagataaa attaaaagat tatgaccatc
     1561 tcaatacaga tgttttggaa cggatacaat caggagagct attagactat ttgttagagg
     1621 aattctcaga taggataaat gatttcttta tttggcgtat ttctctcata gagaatactt
     1681 tttatacgaa gaccccactt gctgcttctg ctaaattgat ggaacagtta tttcaatact
     1741 atcagacacc tcaagatttc caacaaattt taaatgaagt atatagtcat caagaagcgg
     1801 ctagaatatt gagcgaactg ccggaatatc aagaagaaac acttgaaaaa gccaagcagc
     1861 tgcttaatca agatttttta tttgaggagg atcaggaatg aagattttaa ggatcgagat
     1921 tgctgctttt ggaaaatggc gtcaaaaaag ttttgacttt tattcaggca atcagctgat
     1981 ctatggggga aacgaagcag gaaaatcaac gatttatcaa tttattcaag caatattatt
     2041 tggtttccct tcaaaaggcc gtaaaaaaaa ggattataca ccaaaagacg gctcagccta
     2101 tggcggcaaa atatggctta aacatcctgt atatggtgaa tttgctgtcg agcgctacaa
     2161 gcagcaaaat cgcggaaaat cgaaggtgtg gctgggggat caagtgggaa gtgatgagtt
     2221 actagaaaaa ctgatcgcac ctttgacgaa agagttattc cagcaggtat ttactttcca
     2281 gcaagagcag ttgatggaac tagatcattt gagagaaaaa gagctacatg atgcgctgat
     2341 ttctttaggc atcacgggca gttctcaagt attccaaaag cggcaagaat attatcagaa
     2401 agcacaacaa ttattcaaga aaaaaggcca aaaattacta atcaatcaaa aattaaatga
     2461 atggaaagag ctgcaagaaa aaattcatca aaaacaagaa caggaagctt attttggcaa
     2521 gttgatccaa caagaacagg aatacgagtc gcaacagcag cagttattgg cacaattaaa
     2581 agaggcgaac aaacaatttt cattagccag acagcaagaa cttaattttt ctttatatga
     2641 agaatggcaa tctctcaaaa aaaacagcac tacaggtctt cctgatgagc aacttctttt
     2701 agatctggag tctttctcca aacggtatca gcaaacaaat gagcgtttgg aagaattaga
     2761 agcaacactg gaaaagcata gcgggatgga tcagcagtca gccagatatt atttttattt
     2821 agagcaggag caggaactaa aagatctcca gcaacttcaa tttacagcgg aacaattaaa
     2881 cgaagaatgg caaagattac aaattgagac tagagaaaca aattcagaat tgaagatttt
     2941 ggaacaaagg tggcattggc agaaaaatcc gttaccggaa gaatttttat atgaagaaga
     3001 atggaagcat cttctggctg aagaaagaag gattgctgat ctagaaagcc agatttctgt
     3061 gcgtctccaa atagctaaag aacagcaaga gatgattgaa gcagaagtga atgacttgga
     3121 agagacctat ccagcattat tacaaaatca gtcagagata gcaccagtct cttcatctaa
     3181 accgtatatg ctgatggctg gcggagcaat aatcattgca ggtatatttg taccaatgcc
     3241 cttgaaagtg atccttttac taattggtgc aggcatgatt ggctatttct tctacaaaag
     3301 gcaaccaaaa attaaaacta aaaatgattt ctctgagata aaaggaatgt ggcaagaaaa
     3361 actaggacaa cttgatctag ccttagctca attagctgaa gcagaagaaa aacaaaagag
     3421 aactggagaa gaaaaacaga ttcttcagca agaagtatat cgttttgcgg atcatcggca
     3481 cttaggaaag atggatacgc tcaaactcat gaaagaatat ggacaggaag tcatagatta
     3541 tcagaaatta tatggcgtat ttgagcggaa acataaaaga gaacaggaaa tacaagaaaa
     3601 actacgacaa atcgaccaaa aatttgagtt tgttaaagaa tggttaccga tacaagataa
     3661 gacaatttca gaaaaaatgg tattgctttc ccgttttata caagagatgg aacagatgaa
     3721 atttgccaga agctatcagc aaaatacttt attgaagcaa gaaatcaatc aattgaaaaa
     3781 gaaacaaaaa gtatggatac aagaatacag cgaacaattg actcaagcag gcttatctta
     3841 tccttcggaa gtacctgtct ttttacaaaa agccaaacaa gccgaacagc agatgaaaag
     3901 atacaatgaa ttatcggaaa tgcttactcc actatttcct gaagagctga caaaagagaa
     3961 attatctgta agactgcgcc aactgaaaga aaagcaatcc caactccagc aagaagaaaa
     4021 agaagcaagt gaaaatcggc aacgactcca acttcaagta gaagagttgc aaagagacgg
     4081 cacattggac gaactttatc aacaagctag ccggcaaaaa gcaaaattac aggaattact
     4141 ggaggaatgg ggagcacttg aaatcgccgg gacattctta ggtgatcttt caacagagat
     4201 gagtgaaaga caattacccc agttgttaag agcagcaggt gattatttag gtattttaac
     4261 aaaaggaaat tatcagaaga tattactgaa aaatgataca ctgactttaa cagacggata
     4321 tacagaattt cccatctatg agttatctac aggtacgaaa gatcagctga ttatggcgat
     4381 gagatttgct tacctctcat tagaagctaa ccgttcgata tgcccgatca tcattgatga
     4441 tggctggctt cattatgatc atcagcgaaa gtatcaatta gctgaacttt tagctaaatt
     4501 tggagaaaag tatcaaataa tttgtttttc ttctgataaa gaaatggtaa gctattacca
     4561 tgagatgaac caaatcgtaa aacaattaga aggagcaaat aatgaaaaaa attcgtgatt
     4621 tagtaatcga tgagcagttt gaacaatttg tcctgatcaa aagtgcagat gtaagaatcg
     4681 ctaaaaacgg aaaaaaattt attgctttta ctttccaaga tacttcaggt actatcgatg
     4741 gtaaatactg ggatgcttcg gaggaggaaa tcaataaatt tacagccggc acagtcgttt
     4801 ttctaaacgg taaaagagag gtttatcaag gaaaccctca agtaaagatt cttcatatgc
     4861 gcttaacaag ggtagatgag ccaggagagc ctagtttata catggaaaga gcccctttga
     4921 agaaagaaga aatggaagaa gaaatcaatc aattgttgtt tgagatcacc aatgcgcatt
     4981 ggaatcgtat tgttcgctat ctacttaata aatatcaaaa acaatttttt gagtttccgg
     5041 cagcaaaaag aaaccatcat gcatttgcca gtggattagc ttatcatact ttgacaatgc
     5101 ttcacttagg gaaatcaatc gccgatgaat atcctgagct gaatcgagca ttgttgtatg
     5161 ctggtatcat tttgcatgat ttaggaaagg tgattgaact ttccggtcct atgtcgacag
     5221 aatatacgct tgcaggaaat ttgatcggtc atctggtatt gattgacgaa gaaataacta
     5281 aagcctgtct tgcgttgaaa atcagcgaag ctgacgaaga tgttctcgta ttacgtcata
     5341 tggttttagc acaccatggc ttattagaat atggctcacc tgtacgtcca agaataatgg
     5401 aagcagaaat attacatcaa atcgacaata tcgatgcttc gatgcagatg atgttgactt
     5461 cgattcgtca aacagaacca ggaaaataca ccgatcggat tttcggtatg gacaaccgaa
     5521 gtttctatgt accaaaagat ttatagcaaa aatttatttt tttcattcaa aacaaaaaaa
     5581 gcgagacata agtcttcgct ttttttgttt tc
//
LOCUS       DAZUMT010000140         5441 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000140 DAZUMT010000000
VERSION     DAZUMT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.144"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..568)
                     /locus_tag="ACG8FM_002668"
     CDS             complement(<1..568)
                     /locus_tag="ACG8FM_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS5095262.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSN"
     gene            complement(902..989)
                     /locus_tag="ACG8FM_002669"
     tRNA            complement(902..989)
                     /locus_tag="ACG8FM_002669"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(955..957),aa:Ser,seq:cga)
     gene            complement(1134..1700)
                     /locus_tag="ACG8FM_002670"
     CDS             complement(1134..1700)
                     /locus_tag="ACG8FM_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5095263.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            1955..3286
                     /locus_tag="ACG8FM_002671"
     CDS             1955..3286
                     /locus_tag="ACG8FM_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS5095264.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3252..3602
                     /locus_tag="ACG8FM_002672"
     CDS             3252..3602
                     /locus_tag="ACG8FM_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095265.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(3694..4113)
                     /locus_tag="ACG8FM_002673"
     CDS             complement(3694..4113)
                     /locus_tag="ACG8FM_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS5095266.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(4114..4458)
                     /locus_tag="ACG8FM_002674"
     CDS             complement(4114..4458)
                     /locus_tag="ACG8FM_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS5095267.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            4808..5404
                     /locus_tag="ACG8FM_002675"
     CDS             4808..5404
                     /locus_tag="ACG8FM_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5095268.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1513 a         1007 c          930 g         1991 t
ORIGIN      
        1 catttgatag cgagcgataa ttgttcagat taaataattg ataatcaaat ggcggattat
       61 ttgtcaacac accgacagga ttatcatata aatgaagtcc atctttcata ctttcaatta
      121 ctatcgattt ttctttatca gctaataacc agtgtaaagg tgacaagggt agtttttcac
      181 taaaatcgat attgactaga ttgatagtag ccaataattc tttagcttcg tctatggttg
      241 agcattgccc tagtatccat ggaataaact caaatggtgc aacgttatct tttccttctt
      301 gtaattcttt ataatcagca ttgccaggaa aattcagtcc tgccatactc aacccttttt
      361 catttgtagc ctcataatag agtggatagt tggatacccc agctgctatt cctatcattg
      421 cataatgttt atctaaattt tttacctttc taaaatcgaa acgataattt cttggagtaa
      481 tagtgaccac ttcattataa gacatctcat aatcaaaatt ccttccaaaa taatggtctt
      541 tcgttgcata agtgattgcg gtacacatcg tttcccctcc taaaacaaat cgtttttaca
      601 cttttatctt acatctagta ttttttttat caaaaaaagg ccaaagaaga ttgatttact
      661 agcatttata aaaaatagta ctcaggggga atcaaaaaat attgtaggtt aaaagtacaa
      721 caataagggg ctgtgacaaa agtcttgtca cagcccctta ttccgaacaa acggtgttca
      781 aaagcttgca tctgcggtaa taagcccaat caaacgaaaa aataggaaaa gctatttttg
      841 tttggccgtt cttattactt gatgcaggac agcttcttca caacctctaa tcgttgatat
      901 aaggaaggta caggatttga acctgcgcgc cggtattagc cggttcgccg gatttcgagt
      961 ccggtgcatt accactctgc caaccttccg taagtactat ttttaaactt ttttcacgaa
     1021 atgtcaagcc tatttctaat agataaataa cagaaatcgc tttttttagc aaaaaagtcc
     1081 attgacagtt tgcagccaac ggacttaccg atcaaattca tactgataga acactatctg
     1141 attatttcag gattatctaa tgctaaaatc gagtcaattg tccgtttgat cagtgcaatg
     1201 atttgttctt ctgttggatg catgatgttt gatgacatca atgacgctaa gccagtcact
     1261 gctacccatg taccagtatg cagagcatcg acatattcat ccgataattg attgtatctt
     1321 tcatcttctt taactatttc acggaaataa ttgtatgaaa aatcatgcat tttctttccg
     1381 tccccatgtt catcaatgta caatgccatg taaagactat gatgcttttt tgcaaagtag
     1441 atatagttca aaccgagatc aatgatttta tcaccagtac gttcaatagg gaaaacatct
     1501 tcttttagtt gttcaaagat agaatccagc aacgtatttt ttaaatcttc catgtttttg
     1561 aattccagat aaatcggctg tgtcgaaatc cccatctttt tagcaatatt tctcgcagta
     1621 aatcctttaa acccttcctt tgcaacaaca tcataagcag cttttagaat ttgatccttg
     1681 gtatagactt ttcttctcat taaaactccc ccacttcttt actgcagtat atgttgtttt
     1741 attgtaccct atttttagca ttttttgcaa acattttact caaaacgtta tattttaaat
     1801 ttttttcagt cttctttaac ttttttttgc aaaattaaaa aaatttggtt aaattcaatg
     1861 ggttccctta catttttagt tttgacctaa atgatagttg tccaaatctt tcatttcaag
     1921 ttatagttaa agaaaatgaa aaagaaggtg tgtaatggaa aacgaaactg acgttattta
     1981 tattcatcct caaaaacgaa tcgtttcaca gaaaagaaag tatttttatc ttggttttac
     2041 aggtgttttt tttcttttta ttggcttgct ttctaatact cctactgata attggagtgg
     2101 attgctaact attcttacct cgccaagcaa tcttttgaca gattattttg ctttaggtgt
     2161 tttcggaagc gcattcataa atgtagggat tttgacctta ctttctgtgc ttttagctta
     2221 tcggcacaag gtgattttaa acggtccctt atttgcttcg attctaacag ttaccggttt
     2281 ctcttttttt ggcaaaaacc tttataattc tatctcaatc attttaggtg tgtatctata
     2341 cgctgtattt gtcaataaac ctttttctca gtacatcatg atcggactgt ttggtagcgt
     2401 attatcaccg gtcgttagtt atatcacttt tggtatgcgt tttcctttac tgataggtat
     2461 tttattgggg aatttagctg gcatagcaat tggtatactt cttccgccct tagctgctca
     2521 aactttagtt ttccatcgtg gattcacact ttataatatt ggattcacct ctgggttgat
     2581 tgcgatgact tttactgctg ttttgaggtt attcagctat tcaatcgtcg aaaatacatt
     2641 ggttttcaat gaatatcact ttcctctcat ttggattatt tttggctttt tttcgctgac
     2701 agtcggaatt ggtttctatt ataattcctt tcggctttcg gggattcgag aaattttcga
     2761 ttcatctgga aaattgacaa ctgatttcat tgctaattct ggtattggtg ctactttgat
     2821 caacatgggc ttagttggtc tgatgctgtc ttcttatgtt ttgctggttg gcggtcagtt
     2881 aaatggacct gtcatcggtg ctattttatc tgcggtgggt ttcagtgctt ttggttgtca
     2941 tttaaaaaac agttttccga tactagtcgg aatatttatt gcttctttgt ttggaacttt
     3001 tcatgagatc accagtactg gcatgcttgt tgcagctgtg tttgggacag gtcttgcgcc
     3061 cataagcggt ttttacggca gtttttacgg tgtaatagct ggtgtgctac atattgctct
     3121 tgtccataac gtcagcactt tgcacggagg actaaatcta tataacagtg gtttttcaac
     3181 tggtttcgtt gcaggtatcc ttgtaccgat actagataac tttaccgctg tcagaaagga
     3241 aaaaaagaca cttggaaaaa gaattatcaa aaagaatcat cgatgagctt cttacttatt
     3301 tatattccca tgaaataact gaaatacgta ttggagtaga atttgctgag gaagggcttt
     3361 tcatagagat acagggtacg gcagaaactc agccagaaga tttagatgta cttgctgatt
     3421 tgctgaacaa tcctcgggac cctagtatgg aaatctacta tcaagaacta ttaggtaatc
     3481 cccgacatga tcgggaagat tatcatctgt taggtagttt gattgatgac gtggaaatca
     3541 tgtatgccca gccgattatc aatattaaga tctttcgacg tgcttctcat tcctattatt
     3601 aaccctattt tctatttata aaagactgaa tcaaaaaagc agctcctgaa agttcgatta
     3661 aaatctactt tcaggagctg ttcaagcttg acattattga ttttccaatc cttcatacac
     3721 ttcatctgtt acaggttcta aaaactctga attaccagca gtgatcgcca aatgttcaaa
     3781 ccaactgtct tttgtcgcac catgccaatg tttaatccct ttgtgcgtta cgacgacatc
     3841 tccagccatt aatcgtcttg ctggctttcc ttcttcctga taccagcctt ctcctccggt
     3901 cacaagtaag atttgatagc cgtctgtatg gacatgccag tgattgcgac aacctggttc
     3961 aaaagttaca ttacctacac caacagaaac ttcgggatca ttcactaaca tattcagata
     4021 gctttgcccg gtaaatactt gagcgtaagc ttcgtttttt tcacctgctg gaaaaatcac
     4081 gccattgtgt atttcttcgt gttttgtcat catttacgcc tctttcttat cttcgctatt
     4141 ttcttctaaa tagatttctt ttgctaaact aaaagctgac caagcttttg gccattctac
     4201 ataaaaagct aaatgggtaa tgatttctgc tatttcttca ctagtaatgc cattttgctt
     4261 gccaatcttc agatgagctg gcaattgttc aaaatttcct cctgaaatta atgcagatat
     4321 ggtgatgata ctacgctgat gagcagataa ttcttctctg gaccatactt gtccaaataa
     4381 aacatcatca tttaattctg caaattttgg tgcaaaatct cctaattgat ctcgtcctgc
     4441 ggtttgtttt tctgccattt ctcttattct cccttcacga ctgttaggtg attcaactat
     4501 ggttctaagg caactttatt ttctattgtt tcttcgcatt tcattttaaa tgtagtcttt
     4561 cagcctgtcc aatgcttagt ttattcagtc gatatgcttt ttaagcatag ttgctaacgc
     4621 tttttttatt ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag
     4681 atcaatcttt tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa
     4741 atggcgcagt tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg
     4801 atgaataatg aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt
     4861 aggcctatca atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa
     4921 tgcgttaaga ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat
     4981 ccaaatcctg caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt
     5041 cctgatattt ggcccttatg ctggatttat ctataactat attggaatct gcattggttc
     5101 gattatcatt tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag
     5161 cgataagaca tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct
     5221 gtttgctcta gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg
     5281 attgacaaac atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc
     5341 gattttctta tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg
     5401 ataatattta ttttgtaaaa ttagtcattg attgacaatg t
//
LOCUS       DAZUMT010000141         5313 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000141 DAZUMT010000000
VERSION     DAZUMT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.145"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(405..779)
                     /locus_tag="ACG8FM_002676"
     CDS             complement(405..779)
                     /locus_tag="ACG8FM_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5095269.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(802..2202)
                     /locus_tag="ACG8FM_002677"
     CDS             complement(802..2202)
                     /locus_tag="ACG8FM_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFS5095270.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2206..2616)
                     /locus_tag="ACG8FM_002678"
     CDS             complement(2206..2616)
                     /locus_tag="ACG8FM_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5095271.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2634..3125)
                     /locus_tag="ACG8FM_002679"
     CDS             complement(2634..3125)
                     /locus_tag="ACG8FM_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5095272.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3135..3926)
                     /locus_tag="ACG8FM_002680"
     CDS             complement(3135..3926)
                     /locus_tag="ACG8FM_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5095273.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3919..4692)
                     /locus_tag="ACG8FM_002681"
     CDS             complement(3919..4692)
                     /locus_tag="ACG8FM_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5095274.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4848..>5313)
                     /locus_tag="ACG8FM_002682"
     CDS             complement(4848..>5313)
                     /locus_tag="ACG8FM_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5095275.1"
                     /translation="IQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTI
                     EGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDG
                     ITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1698 a         1067 c          697 g         1851 t
ORIGIN      
        1 taggagtgag catttttcga aaaatactaa aaatttttca aaataaaaaa aggcatacag
       61 acaataaact gtctatacgc ctcacagact ctattctata catctaattt tggctgtcct
      121 gcaaaaaata tgtgttagcc tccatcgaaa gatcaaactt tggaaaacat cattataact
      181 ttgcttggta agagctttgc taaaacatga tatgacttgt atacaaaatg tggagtgtat
      241 tccaataaat aggaaggatt acaaaatttg aaaccaaata agtaaaaaat actgtcactt
      301 tgaattaatt tgaaatagta aattctcatt aaactattga aatgctgata ccaaaaaatg
      361 aagtactttt cgttcatacc aacttagagc acttcgttat ctatttattt cttcatttgg
      421 cttatctcta taatctcatg atttggtccg tagaaattaa agtaacggat tccattttcc
      481 cagaacggta attccataat atttttatct aataattcat atcctaaatc ttgaattgaa
      541 ttgaatacct catctatgtc atccgtatct aatgctagat gatcaatggc tcctctacac
      601 tcagtagttt tatcttccca tatttctatt atcgttccat tcaactcgaa aaagccgact
      661 ttacttccca cattcatttc ttcattaatc agactaaacc ctaaggattc ataaaagatt
      721 tttgaagctc caaaatcttt tgttggaatt ccaatatgtt gtaaaccatt taatttcact
      781 tacatcatcc cttcaattgt tttaaaatcc acattcataa tttattgaat agtctagttt
      841 atctaccttg attgctccct ccgtaacagt catacttaat gctgttccga ctttatcata
      901 cattctagta cttaaagcaa cattatcaat atataaaaca gctatcgtat catcaacaat
      961 taactttagt tcatattctt tgcctatttc caaaaatagt ggtcgttgca gccccttatc
     1021 aaacatttga ggccaaggat agttcggagt ttttccgaaa gtaagctgat tattaatcaa
     1081 tgaaatacga aattcgtatc cttcttgagt atcactatct acaaataatt ttaaagcaaa
     1141 ctcctttgtt aacgatgtaa tagtcaaagt tgtttcaaat agaaaataat cttcgatttt
     1201 aggaacaatt actctttctt tttttccaaa ttctttctct aacttaaaac ttgaaaatgg
     1261 agtgatttct ttaaaattct caattatttt ttcaggcagt ttaacaccaa gagttttgtc
     1321 tgtcttttga ataatttgat gtggaacaag tgcaccgccc caatcccagt tattttgatc
     1381 gttctcccct tcttttgttg ccacccagcc agttagaaaa cggccagttt cattagttcc
     1441 gactgttctt gctgcataat aggcttttcc gtcgaaagct tcgtctactg gtgatttcca
     1501 atctgaaaat aatcctgctc cgactcgata tttagtcttt ttatcttcac aatactctga
     1561 aaaaagtaaa taccacgttc catcaatttc aaatatatca ggcatttcaa tcatgttaaa
     1621 ttcattatta gcccagtagt ctcctttgaa attccaatct tttaaatctt cggattcaaa
     1681 atagaccaat ctccccgtcg gctttttctt acttgctggt tttctagctc ctagtatcaa
     1741 aacgtatttt ccatgatctt gatcaaaaat tgcaaaagga tcgcgccaat cattcttatc
     1801 atatccttct tgaggaacaa gttttgctgc ttcgccatcc tttttccaat gaattaaatc
     1861 tttagaactt gcaatcatta acacttctga agtttttcca tccaacttag ctttgcgatt
     1921 atgtccagta tacaaagcta aatactcatt gattccttgg acaatacttc cagcgtaaat
     1981 gtattgatca atgtcatcgt tgccaccacg atgaattact tccccgtatt cttcaaattt
     2041 cacaaaatca gtggttgtaa ctagtgacca cccaaaagga tcagttaaag gtttaggatt
     2101 tctcgtatct cgttgatgaa atagataaaa agttccatct ttataaaaag gcatgcaatc
     2161 cccaaaccaa aaatctttag gcttgtaaaa aattttttgc atttatcact catcctcttc
     2221 gttagtatgt ctttgcatat ctgcaagaca cgcaattgta ttcatatttc ttagattttc
     2281 gatgatatca tcgctttttt ttccttgtaa tctcaaattg acagcctcaa tcaaaattcc
     2341 taaattcatg ccaccaagaa gttgaaaccc attttgaaat acttcacttc ttttagcaaa
     2401 ttcgttgaaa ggtgttccac caatcaagtc agcaaatacc ataacttctt gattttttac
     2461 ttgagaaact attttttcaa tgttttcacg gaattcttca atgcctgcat ctccaagtgt
     2521 taccacatga caatttaaat tatcgccaaa aaacattttt aatgtatcta caaatccaat
     2581 ggcaatattt ccatgagatg taattataaa ctccatccac tcatcctctg ttcttaataa
     2641 ataaaatcct ttaaaaattc ttttgaatct ctaggagttt gtatcactgt cacttcaact
     2701 ccatcactaa ttagttcttt aaaagcctct ctctcttctt cagtaatatt tatcgtcgga
     2761 cggatgcgtt ctgttccatt ccttcttgat aaatttccta cgtttacact tgttattggt
     2821 aaaccgttat caatcaaaaa tttaacatcg attggagaaa ctgcaacaat aagaactttt
     2881 tgtccttcat atttaccaga aagaatattt ttaactgcta cttcccttgt aattattgaa
     2941 gttctaacat taggtggtgc agcgaggcga actacagctt tttttacgtc atcattggct
     3001 acttcattat taataaccat aattctgtta gcactcaatt gatttgtcca ttgtgttgct
     3061 acttgtccat gaatcattcg atcatcaata cgaacatgta taataccttt cattttttca
     3121 tccatattca acacctactt caaaatattt aacgctctta acgcaataga aactactaag
     3181 attaatagaa ttacataaac tggtttaacc tttcgtccta acaaataata cacaattgct
     3241 actacacatg caggtaataa accaggcata acttgatcaa agatttcatt tccggtcatt
     3301 gttaagtcgc cttgtttaaa ttcaaagcca acattcactt ttacaactgt ggcaatgagt
     3361 gcaccgacca ccattaaccc taaaattgat actgcatcag taatagactt taattttgtt
     3421 tcaatttgag ttagtaggct cgcacccgat tctttcccag caaggtataa aggataagca
     3481 gtaatcctta ataaaattga tgccagtatc cacaaaataa ttccaacaaa actaccttcc
     3541 aatgccatat tagctgcaat tgctccaaaa atagtccatg gaataactac aaatacacta
     3601 tctccaattc ccgctaaagg acccattaga cctgttttca gagcacttac tgcttccaaa
     3661 gacttatttt tttcttcttt ttcaattcca acattcatac caataatgta aggcgctgta
     3721 tacggcgtag tgttaaaaaa ttgtaagtga gctaaaacgg attgtttcat tccttcagga
     3781 tcatctttat atgtgtcctt gataaaagga agcatagaat acgcataacc caacccttcc
     3841 attttttcat aatttaatga agttgcacca ttgagaatcc accggaaaat tacttgtttg
     3901 tcgatttttg tcagtttctt attcatcttc gtcaaaacct cctgttgcag ctggtgctac
     3961 ttgagtatta tttgatctat ttttatatac gattagtgcg attgcagcgc caagtaacga
     4021 aatccctaaa attggtattt ggaaataaac tgccaaaacg aaaccaacta acaaataggc
     4081 aaagtaatct tttgtaggta aatatttaag tagcattcct atcccaacag caggtaatag
     4141 tccccctgct acctttaaac caccggtaac ccattcagga atataattga ttgcacttcc
     4201 cactaacgtt gggcctaata tcacagttaa caaaacagga attccagata caaagcttgt
     4261 attaattgat gccaaatatt gcatccactc taatttttga taatcttctt tttgtgcgaa
     4321 tttttctgcc ttttgttgaa aaaaaatgtt tgttgaccac tttagaacat ctagttgtac
     4381 aattaacatt gctaccggta ttgctaaagt tattcctata gaagcatcct ggttagttga
     4441 gattgctaaa taagtaccga tcaatgctgc tgtttgataa tccggtactg atgcaccacc
     4501 aaaactagta atacctagtg tcattaattg caaagtccca ccgatataaa gtccagtttg
     4561 aatatctccc ataataagtc cggtcaagaa acctgcaacg acaggttggg aggttccgat
     4621 tttgggccct tccttatccc agttaatgaa aaatccatac aacacaacta aaaatgtttg
     4681 ccaaaacgcc attttgttct ctcctttctt cttatacttt tatataagca attactgtgc
     4741 caactaacat tatccaataa agcgggaaaa tacagtaaat attaattaaa tgagcgtcta
     4801 aatatacttt ggacaccctt tcattttttt agacgctagt atgctattta gaaaatagtc
     4861 tgctcaaaaa ttgtatagat taactcatac tctcctttgg gtatttcaat acagtaaata
     4921 tctttgattt ctgaaaaaaa atcttttgat acatttaaaa aagtatcaaa tagttgcgct
     4981 ccctctttca aatgttcatt tgttattcca tcaccaatca taattcgttc aatcattcca
     5041 gaaagatgta gaaatagatt aattctcaga aaacttggga ttttgacatc gtaatatttt
     5101 tcatacaaat atagtacttt ttctatctca ttgaatactt ttttggaatc taaaaagcga
     5161 agcttagtac tagcgccttc aatagtaaag agtttaacta attcatttaa gcagaactca
     5221 cagctatccg agtccatata cttatttaat ttttctaagg tttgattccc attaattaga
     5281 ttttctaaat ttaaacaatc gattccttga atg
//
LOCUS       DAZUMT010000142         5311 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000142 DAZUMT010000000
VERSION     DAZUMT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.146"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8FM_002683"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8FM_002683"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS5095276.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8FM_002684"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8FM_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095277.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8FM_002685"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8FM_002685"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5095278.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8FM_002686"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8FM_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095279.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8FM_002687"
     tRNA            3917..4000
                     /locus_tag="ACG8FM_002687"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5311)
                     /locus_tag="ACG8FM_002688"
                     /pseudo
     CDS             complement(4240..5311)
                     /locus_tag="ACG8FM_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DAZUMT010000143         5207 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000143 DAZUMT010000000
VERSION     DAZUMT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.147"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1368
                     /locus_tag="ACG8FM_002689"
     CDS             <1..1368
                     /locus_tag="ACG8FM_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095280.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
     gene            1355..2989
                     /locus_tag="ACG8FM_002690"
     CDS             1355..2989
                     /locus_tag="ACG8FM_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS5095281.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3164..3997
                     /gene="msrA"
                     /locus_tag="ACG8FM_002691"
     CDS             3164..3997
                     /gene="msrA"
                     /locus_tag="ACG8FM_002691"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5095282.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4077..5066
                     /locus_tag="ACG8FM_002692"
     CDS             4077..5066
                     /locus_tag="ACG8FM_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5095283.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1825 a          806 c          951 g         1625 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat tttttaaaat agttttccat aaaaggtaaa ttaacttttc
     3001 atttataaaa taaattgatt aaaaaggaga atctgtcgat aaggattctc tcttttttat
     3061 acgaaaaatc tgtaggggga tgaagtaatc gcttatttcc tcactttatg ataaattaca
     3121 gctaaagtca ctagcttgtc ttcttcggaa aggaggagaa tctatggatg aggaacaaat
     3181 attcattcaa acactttata atctgatttt gaatccgaat acacgagatt gggaacgaaa
     3241 agtattgatt cagacaaaga atgacataag agaaaatata tccgttaaag aacagttatc
     3301 aaaactagaa gcaacactac gtccattagc tatccgaatg aatctgacgc ctgatgtaat
     3361 ggacttttat ttattgatta cggaaggatt tgataaggag caaaaatatg acttttccaa
     3421 gcatgccatg caagatgctg actatcaaga acgtgcagta tttgcagggg gatgtttctg
     3481 gtgtatggtc gagccgtttg aatcgaaaaa agggatccta tctgttttgt caggatacac
     3541 gggcggacat gttgaaaaac caaattatga tcaagtaagt ggtggttaca caggtcatgt
     3601 ggaagctgtg gagatcattt atgacacaag agaaataagt tatagcgagt tgctgacaat
     3661 ctattggcaa attactgatc ccacagatac atttggacaa tttcaagata gagggaagca
     3721 atatcgtcca gtgatttttt atcaaggcga acgacaaaaa gaattagcgg aacaatcaaa
     3781 acagaaacta gacagttcag gaactttcca ccaaccaatc gtcacaaaaa tcgaaccagc
     3841 cggtacgttt tggccagcag aaaactatca tcagcaattt tacaaaaaac aaccgaaacg
     3901 ttataaaaaa atccagcaag cacgtaatca atttctgatc tatcaacgag taaaaaacaa
     3961 atggcaaaaa aatatacgaa aaaatcattt tgattaactc cttttcttta aaatgtagta
     4021 aactaaaaat agaaaaaaga aagcgtttta aaaagatttg atgaggagga gaaaaattgt
     4081 atattgcaga tagtgcacgt tatgaaaaaa tggaatatcg taaatccggt cattcaggtt
     4141 tgaaattacc gattttatct ctcggattat ggcaaaattt tggtgattat gatcctatcc
     4201 ataatcaaag agagatttta agaggcgcat tcgacatggg aattacccat ttcgaccttg
     4261 cgaataatta tggaggccct gctggagctg cagagaaaaa ctttgggcgt atttttagag
     4321 aagattttca agcctaccgt gatgaactga ttatttctag caaagcaggt tatcatatgt
     4381 gggaagggcc atatggggaa tggggctctc gtaaatccat catttcaagt tgtgaccaaa
     4441 gtctccaaag aatggggcta gattatgttg acatcttcta tcatcatcgc ccagaccctg
     4501 atacaccttt ggaagaaaca gcagaagctt tgatgcaact tgtaagacaa ggaaaagcat
     4561 tatatattgg tatatccaac tataatggag aagatacaaa aaaaatgact gaaattttaa
     4621 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     4681 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     4741 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     4801 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     4861 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     4921 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     4981 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     5041 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     5101 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     5161 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaatttt
//
LOCUS       DAZUMT010000144         5199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000144 DAZUMT010000000
VERSION     DAZUMT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.148"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACG8FM_002693"
     CDS             25..696
                     /locus_tag="ACG8FM_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5095284.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACG8FM_002694"
     CDS             755..1195
                     /locus_tag="ACG8FM_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095285.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACG8FM_002695"
     CDS             1207..1875
                     /locus_tag="ACG8FM_002695"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5095286.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACG8FM_002696"
     CDS             1939..2562
                     /locus_tag="ACG8FM_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS5095287.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACG8FM_002697"
     CDS             2618..3004
                     /locus_tag="ACG8FM_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS5095288.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACG8FM_002698"
     CDS             3217..4608
                     /locus_tag="ACG8FM_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS5095289.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1728 a          861 c         1095 g         1515 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacag
//
LOCUS       DAZUMT010000145         5087 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000145 DAZUMT010000000
VERSION     DAZUMT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.149"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8FM_002699"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8FM_002699"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFS5095290.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8FM_002700"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8FM_002700"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFS5095291.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8FM_002701"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8FM_002701"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFS5095292.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACG8FM_002702"
     CDS             complement(4028..5044)
                     /locus_tag="ACG8FM_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5095293.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DAZUMT010000146         5039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000146 DAZUMT010000000
VERSION     DAZUMT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.150"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..1175)
                     /gene="xerS"
                     /locus_tag="ACG8FM_002703"
     CDS             complement(90..1175)
                     /gene="xerS"
                     /locus_tag="ACG8FM_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS5095294.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1524..1719
                     /locus_tag="ACG8FM_002704"
                     /pseudo
     CDS             1524..1719
                     /locus_tag="ACG8FM_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3251..3769)
                     /locus_tag="ACG8FM_002705"
     CDS             complement(3251..3769)
                     /locus_tag="ACG8FM_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS5095295.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4136..4384
                     /locus_tag="ACG8FM_002706"
     CDS             4136..4384
                     /locus_tag="ACG8FM_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095296.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            4887..>5039
                     /locus_tag="ACG8FM_002707"
     CDS             4887..>5039
                     /locus_tag="ACG8FM_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095297.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         1672 a          823 c          775 g         1769 t
ORIGIN      
        1 ataaaaatga taatgaagct tgcctttaaa gttgggcttt tatctaattt tagagacagg
       61 cttcattatt atactatttt ttactacatt tacaaagcgt tcaaagcatc aattgtagct
      121 gcattgtcta tatgagtata taaagttgtt gtatttgtac ttgtatgtcc caattgttgg
      181 gcagttaata ccaatgaatt tgtctgttga tagagtctag tagccaaagt atgacgtaat
      241 ttatgtggac tgacttggac tctgaatcct tttgaatatt ttgctaccat ccgttcgatt
      301 gcaataccgg atatacgctt ggcttctcct tgataaattg ctagaaataa agctttttcg
      361 ttttctgtta ctttgtatcg ttctttccga atagctagat aattagctaa atattccatt
      421 gcaaatgctg ctatatttac tgcgtctctc ttatttcctt ttctagttac taccacatta
      481 ttcttttcta aagataggtc ctctaaatta atatttacaa cctcggagag tcttaatcca
      541 gatcctaaaa tcaatgcatt gatcgccagg tctctttcta catctcgttg ataatagatc
      601 aaagctcgtt tgctcaaagt ttttttatat tcattggcaa tataatctag ataattttgt
      661 gtatcttcgt ctagaaataa ttttttctcg attgcgtttg cacgggcagc taacgtagtt
      721 ttatctgtga tagttgctac ttgatccata ggattatttt ctaagtatgg ttttcctaaa
      781 ggattattgg aacttttcga aagatattta aatagaacct ttaatgctgt gatatttcta
      841 ttgatagtag aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc
      901 tcttttttta cattttcctt tgtgcgacgt ttcaaatgag atttgtacaa gtttagatca
      961 taggcagtta actgtcttat cgaggtgatt gaagtatcgg gaacgataga ctcactcaga
     1021 agccattcat aaaagagctt ataatttttt agatattcta aagtagttcg aggtgattga
     1081 tctttatcct cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt
     1141 gtatctattt tttgatatat ttctgtattt ttcatgttat tcatcctttc gataagtagg
     1201 ggagaggagt atctctataa ttggactata ccaattatag agtgttttgt caattggtat
     1261 actctgaaat ttattaattt atttcaaaaa agcagataaa taggtataga tttaataatt
     1321 taaaataaat ggtatacatg aataggaaag gaaacagaag gaggcaatct taatttttta
     1381 aattgtctaa attatataga aagaacaatc tttctatata attagacata agagagacta
     1441 cggttgattt gatattaaca agcgtatatg aatgtatata caaataaaaa aggggaggga
     1501 gaagttaaag aaaattcaga gaagtggggc actcagtagt attaacaatt taaaaggaat
     1561 ttcttaaaga aaatgggttg catcctggag atacaatttt tttagataaa gaaaaaattc
     1621 aagcaacaat tcaaaaaaat cttaactgct gagattgatg tagcaatatc tcaatcatca
     1681 acaaagtacg atcctgcctt tcaaaagctt attgataaat gtttattcat tataaaaata
     1741 aaagaaaaat ttatgtcagc ttttttcatc aaagtggtaa tgataattcg tctattaatt
     1801 gtatttttta ggaaatatgg tataattgtt ttaacgaaaa ggtaattcat gatacaccaa
     1861 aaaccactag ctggcgggct agtggttttc ttttacgttc tattcagatt aacgggtagg
     1921 cttactgtct tttttggcaa taattttgca atcaagccat ttacagatgt aatgcgcgga
     1981 tacttctgct aagattgcaa aaaacatatc gaaaaggtaa ttcataatac aacctcctcc
     2041 ccttgccata ataggccggg gtagcagaca gcataaatat tataccataa ctgaaaattt
     2101 cattttagta catttcaata tattgatgaa ccagcacaat aaaacatgcc atgactaaca
     2161 gaaaaaaact tattaaacca gatataactt gtaaacatcc aactatatag aaaacgtaaa
     2221 ttttctatat agttggatta agataacgac tatttatttt atcatatttt tactataccc
     2281 tatactttta cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg
     2341 taagttagat taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact
     2401 tataaattga tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt
     2461 tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac
     2521 ttatgaaatg aaagaccttt tacatcttgc agaaatcgtt actgggatta aactaagcaa
     2581 taaaaactaa tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata
     2641 aatgacgatt tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc
     2701 agtatcagta ataggatagg cgataggcca catacccttt gatggttaga ataaactgct
     2761 caaaatctgt atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa
     2821 ccataagcgt tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata
     2881 taattaccgg acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa
     2941 ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct
     3001 agtctcctaa gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt
     3061 catggtttat ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca
     3121 ttctagggga gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg
     3181 tagtcgtatg ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt
     3241 aaacgggaat ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa
     3301 atggagcaac tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg
     3361 cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa
     3421 tcatcatgtt aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta
     3481 gatgtgcata ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt
     3541 gatcagaagg cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta
     3601 aattcgtcca accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa
     3661 ctcttttatc cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag
     3721 tcctatttat ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc
     3781 cataaattcc taattgtctc ctttaattca tctataattt catccacgat attttaatcc
     3841 gttctacaat gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa
     3901 aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga
     3961 atatatttct tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca
     4021 aaatcatgtt aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg
     4081 ttaagtttag ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt
     4141 aaaactcaaa atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg
     4201 taaaaaatat tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa
     4261 acagctacaa aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc
     4321 atattcacgc ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt
     4381 ttaggaaatt gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc
     4441 accagatatt cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca
     4501 ccgcgaattt actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat
     4561 taagggatca agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta
     4621 aacaattata gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac
     4681 tttcttattc tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt
     4741 acgagtacta ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc
     4801 cccacttagc ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag
     4861 aagaaaacat accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag
     4921 aattcaagca acaaatcgtc aatctttact tagctggaaa gccacgtgta gaaatcattc
     4981 gagaatatga actaacggct tcagcatttg acaaatgggt aaagcaatct aaaacgagt
//
LOCUS       DAZUMT010000147         5017 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000147 DAZUMT010000000
VERSION     DAZUMT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.151"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..165)
                     /locus_tag="ACG8FM_002708"
     CDS             complement(<1..165)
                     /locus_tag="ACG8FM_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095298.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
     gene            complement(367..762)
                     /gene="panD"
                     /locus_tag="ACG8FM_002709"
     CDS             complement(367..762)
                     /gene="panD"
                     /locus_tag="ACG8FM_002709"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFS5095299.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(772..1620)
                     /gene="panC"
                     /locus_tag="ACG8FM_002710"
     CDS             complement(772..1620)
                     /gene="panC"
                     /locus_tag="ACG8FM_002710"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFS5095300.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1635..2462)
                     /gene="panB"
                     /locus_tag="ACG8FM_002711"
     CDS             complement(1635..2462)
                     /gene="panB"
                     /locus_tag="ACG8FM_002711"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFS5095301.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2474..3334)
                     /locus_tag="ACG8FM_002712"
     CDS             complement(2474..3334)
                     /locus_tag="ACG8FM_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFS5095302.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3572..4741
                     /locus_tag="ACG8FM_002713"
     CDS             3572..4741
                     /locus_tag="ACG8FM_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5095303.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1611 a          942 c          633 g         1831 t
ORIGIN      
        1 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
       61 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat cgacgatttg
      121 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa atcctccagt
      181 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcac
      241 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
      301 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      361 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      421 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      481 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      541 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      601 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      661 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      721 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      781 ttcaaatgta aaattatcta ttaatcttgt ttttcctata aatactgcta ttgcaactaa
      841 aactgatttt tcaatataat ttactctttg taatgataaa gaatcaacaa cttctacata
      901 atcaatcttt gccaaattat aagtatttat atttttactt attatctcaa ttatttttaa
      961 gctgtctctc tctccattat ttaaagcttc cttagctaaa gttaaagatt tatttaaaat
     1021 agtagctgaa gatctttcct ctaaacttaa gtatgtgttt ctagaacttt tagctagtcc
     1081 atcctcttcc cttataattg gacaccctac aacgtctata tcaatattaa ggtccctaac
     1141 cattctttta attacagcta actgttgtgc atccttttct ccaaaataag ctctatctgc
     1201 tggtattatg ttaaataatt tacttacaac agtgcaaacc cctctaaaat gtattggcct
     1261 actagctcca cataaacctt ctgttaatcc agttatatta acaaaagtac ttgcattgtc
     1321 acaatacatc tcttcaactt caggattaaa tattgctgtt gctctacctc ttgtgcatac
     1381 ttctatatct ctttctaagt ctcttggata actacctaaa tcttcatttt tcccaaactg
     1441 agttggattt acaaagatac ttacaacaac tttatcattt tcttttgatg cttttctaat
     1501 taaactttca tgaccttcat gtaaatatcc catagtagga actaatccta cctttaagcc
     1561 ttgttttttc cactctttta cttgacttct aacatcattc actgttttaa atataaccat
     1621 atttcttgac ccttttaata taatttttct aaaacatcat cacttatttt aaacccatgt
     1681 tctggacctg gaaaaactcc atcttttact tcctctatat attttgtaaa tgctttattc
     1741 atctcttctg ataaattagc atatttcttc acaaatttag gggtaaaatc actatacatc
     1801 cccaacatat cttgatatac aagaatttgt ccatcacacc ctgcacctgc tccaatacca
     1861 attgtaggta ttgatatctc tttagaaatt aactctgcaa gcttagttgg aacacattct
     1921 aacactacag cgaatgctcc tgctttttct acagctttag cagctcttat taattcttta
     1981 gcagcttcct tgtcctttcc ttgtacctta aatcctccaa atgcattaac agattgaggt
     2041 gttaaaccta tatgcgccat aacaggtata gaagccttta ctatagcctc tattttatcg
     2101 catacttcta tccctccttc taatttaaca acctgagctc gtccttcttt tattaatctt
     2161 cctgcattaa ctactgaatc atatactgaa gtttgataag acataaatgg catatctgca
     2221 acaactaaag tgttcttagc tcctcttgta actgctctag tatgatgaat catgtcttcc
     2281 attgttactg aaagtgtatc ttcatgtcct agacatacca taccaagaga atctcctact
     2341 aatattccat ttatcccagc ttcatcaatt atttttgctg ttgaatagtc ataagctgta
     2401 agcattgtta atttttcatt tcttaattta gattctttaa atgtaactgc tgtatttttc
     2461 atctatttct ctcctaactc tctcctaaat acttttctaa ttcactataa tctctattaa
     2521 tattttttaa catagctatc tctaatacat tttttgataa tagcatatat aaagctctgt
     2581 cttcatctga taaaacttca cagtgacctt taattgttga aatatcacaa cgttcaactg
     2641 gccctgtcaa actttctata attccttttt cttttatatt tctaatatta aattcaatta
     2701 atggatataa agcttcaatt gccatttttt cagtaaatcc acattgaatt agatatgtaa
     2761 cgcttgtatt aataagtcct agcacaaggt tactagatac tactgatgcg gcatgataaa
     2821 gagatttatt ctccttagat acaattacag tactattacc taactttttg aagaaatcac
     2881 ataaaaactt tgcatatttt tcatcgcctt caattgttat aaaagatttt tttaattttt
     2941 tgtatgaatt atatttatct gatattgaaa acataggatg gacagaatag ccataagcac
     3001 catagtcctg tatatttgaa aagatatctg aagatattgc accactgcaa tggcagatta
     3061 atttgttttt gatttgataa tttttaattt cttgccatac ctcctgaatt tgaccatcag
     3121 gagtcgtaat gaaaatagta tcattttcat ctactaattc tcttaagttt aaatattgtt
     3181 tactagatgt aaaattcgaa gctgctaatg acgagtcttc gcttttacta taaaatcctg
     3241 ttacttgtat tttatgttcc tggaaatact tcccgaatga acaacctacc ttgccggctc
     3301 caataaaacc aacgttcata attatcacct ccataaagaa gtgagactac aagtcccatt
     3361 cttacgatat gagcttaaaa ataaaaatta aatcaaatat acagtatgtt tttttcaaat
     3421 atcatccata gttaatttat cataatatcc catattttaa aataaaagat agcgtaaaat
     3481 attttgtgta aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta
     3541 ccacacagaa attttatagg aggactttct catgactaaa tttactatag aaattatgca
     3601 aacactactc aataaagggg atttagatga attatttcag gatcatttag agcgagcgat
     3661 taattcactt ctacaagcag agctaactgc ttttttagat tacgaaaaat atgatcgaaa
     3721 tggattcaat tcaggaaatt cccgtaacgg gaattattca cgtacattta aaacagaata
     3781 tggcgagttg aatttaacta ttcctagaga tcggaatggc gaattttctc aacaaacact
     3841 accagcatat aagcgaacca atgattcatt agaaacgacc attatccaat tatttcaaaa
     3901 aggcattact atggctgaaa tctccaaatt aattgaaaaa atgtatggtc accactatac
     3961 gccacaaaca atctccaaca tgagtaaatt ggtggctgaa gatgttttag cttttaaaga
     4021 aagaacgtta gaagccaatt attccgttat atttatggat gcgacgcata tccctgtgaa
     4081 gcgacagact gtttcgaagg aagcagtgta tattacgatt ggtattcgtt tggatggaac
     4141 caaagaagtt cttgggttta ctattgcccc aactgagtct gcttatattt ggaaagaagt
     4201 tcttcaagat cttagaaaac gtgggttaga agaagtttta ttagtagtga cagacggatt
     4261 aagctgtatt gaagaaagta tccatagtgt gtatccgaat gcccaatttc aacaatgttg
     4321 tgtgcatgta tctagaaata tcgctcataa agttcgtgtt cgaggtcgaa aagaaatttg
     4381 tgaggatttc aaattggttt accaagcgaa ttcaaaagaa gaggcattgg atcacatcga
     4441 ctttatgact aggaaatgga aaaagcagta tccaagagtc gtcaatttac tcttgaatcc
     4501 tgccctatta accttttata atttccctca cgccatcaga cgaacaattt attcgacgaa
     4561 cctgattgaa ggctttaata agcagctaaa acgatatact cgaagaaaag aacaattccc
     4621 taatgaagaa tctctagaga gattcttcat ttctcaattt aatcaatata accaaaaatt
     4681 tttaggtaga attcacaagg gatttaaaga aattcaggat acattagagt cgatgattta
     4741 actgtaatta acagaatgga ttttccattt acacataatt cttgacgcta ccataatcta
     4801 aataaccatc atattcatct actacttata taattgagtt ttattaaact ttttaagttt
     4861 ctattctatc aaatatttaa acaatctctg catccgaaaa gactacctcg tatagtttta
     4921 ctaacaatat ttttattagc gtgttcaaat cctgatttct tgtatcaatt attttctgtt
     4981 cactttcaat aagaaaaaag tctttacttc catggat
//
LOCUS       DAZUMT010000148         4972 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000148 DAZUMT010000000
VERSION     DAZUMT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.152"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8FM_002714"
     CDS             complement(<1..182)
                     /locus_tag="ACG8FM_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5095304.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8FM_002715"
     CDS             517..951
                     /locus_tag="ACG8FM_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5095305.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8FM_002716"
     CDS             974..1294
                     /locus_tag="ACG8FM_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095306.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8FM_002717"
     CDS             1435..1626
                     /locus_tag="ACG8FM_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095307.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8FM_002718"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8FM_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS5095308.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1981..2124
                     /locus_tag="ACG8FM_002719"
     CDS             1981..2124
                     /locus_tag="ACG8FM_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095309.1"
                     /translation="MIQNNTLCEMAQAVLWIYPVKSVSILKLEEKLAIIKVKEGVGFS
                     WIM"
     gene            2112..2375
                     /locus_tag="ACG8FM_002720"
     CDS             2112..2375
                     /locus_tag="ACG8FM_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095310.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8FM_002721"
     CDS             2399..2587
                     /locus_tag="ACG8FM_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095311.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8FM_002722"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8FM_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095312.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8FM_002723"
     CDS             3070..3441
                     /locus_tag="ACG8FM_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5095313.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8FM_002724"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8FM_002724"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8FM_002725"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8FM_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095314.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8FM_002726"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8FM_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5095315.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1735 a          780 c          975 g         1482 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tc
//
LOCUS       DAZUMT010000149         4961 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000149 DAZUMT010000000
VERSION     DAZUMT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.153"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..803
                     /locus_tag="ACG8FM_002727"
     CDS             117..803
                     /locus_tag="ACG8FM_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS5095316.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            805..1749
                     /locus_tag="ACG8FM_002728"
     CDS             805..1749
                     /locus_tag="ACG8FM_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS5095317.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1948..2592
                     /gene="pcp"
                     /locus_tag="ACG8FM_002729"
     CDS             1948..2592
                     /gene="pcp"
                     /locus_tag="ACG8FM_002729"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS5095318.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2671..3948
                     /locus_tag="ACG8FM_002730"
     CDS             2671..3948
                     /locus_tag="ACG8FM_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095319.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4206..>4961
                     /locus_tag="ACG8FM_002731"
     CDS             4206..>4961
                     /locus_tag="ACG8FM_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095320.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         1614 a          892 c         1055 g         1400 t
ORIGIN      
        1 aaaaaaataa cgaaaaatat ttaaaaatag tgtctatttc gtgagaaaac aactatattt
       61 tttatgtgtt ttgctttata attaaaaaaa tattgtcatt ttatggagga aaatatatgg
      121 aaaattatct tagcctttta ggcgtcttga ttgttattct cgggtttgca tttaaattag
      181 actcgatatt gatcgtaatg gttgctttag ttgttacagc tttgactggc ggtcttggca
      241 ttcaaggaat gttagaactt ttaggtacaa gttttgtaaa caatcgcggg atggcgattt
      301 ttatcatcat tatgttggca acaggaacgc ttgaaagaaa tggtctgaaa gaatcagcgg
      361 cttccttgat caagcgattc aagaaagtat ctgctggtat gattgtagat atttatggga
      421 tctttcgaat gatctttgcg gcttttaacg tgagttttgg tggagttgct ggatttgttc
      481 gtccaatcat tttaccgatg gcattgggga ccatcgaatc aaaagggtta gaagtccatc
      541 ctaagcatga agaagaacta aaaggtatgg cttcagctat ggaaaatgtc tgctggttct
      601 ttggccaagt actattcatc ggaggagcag gggcgttgct agtccaatca acactgaaag
      661 atctaggata tgacgtaaca ttgggacagc ttgctttagt ggaagttcca gtggctatca
      721 cggctttagt tgtggcaagt gtctacttct atcttaaaga aaagaaatta gcgaagaaat
      781 attatccagg aaaggggcaa taagatggac ttttttacaa atcctgatat tttattaggc
      841 gataaattac tggagatcct ttatatcgtg atgggactcg tattgattta cactggagta
      901 agaaatttaa cagataaaac caatcctcat cgttatgggt cagccttttt ctggacggtc
      961 ttaggggtag ttattgcagg aggaagatgg cttccggcaa tcgtgaacgg tggcttgatc
     1021 tttgcgatga ccataccagc aattgctaag aaagtcagca aaggagaaag ccggcttcca
     1081 tcaaaagaat atatggaaaa aatgtcagac aaactaggaa tgaaaatttt tattccagct
     1141 ttgagtatcg gtgtctttgc gatcttgttt gctctattta ccaatttagg tgctttagtc
     1201 ggcgtgggcg tcggtgtttt tgcagccatg ctaatcttga tgttcttttc tcgagataat
     1261 cgaccaagta catttttaga tgatgcagca gatatgctgg gcacagtagg gccgttaagc
     1321 atgttgccaa tgcttttagc ttctctaggt gcggtattta caagcgctgg agttgggaca
     1381 gtgatttcca atgcagtagg tacgatcgtt ccagaaggaa atgtgaacat cggcatcatc
     1441 atttacgctc ttggcatggt gatatttacg gtaatcatgg ggaatgcatt tgcggccatc
     1501 accgtgatga cggttgggat cggtgcacca ttcgtattta gtcatggagc gaatccagcg
     1561 ttaataggaa tggttgcatt gacttgcggt ttttgtggga ccctgctgac accgatggct
     1621 gctaacttca atatcgtgcc tgtagcgatg ctggagatga aagacaaata cggagtgatc
     1681 aaaaaccagt tgtttattgc gttatttatg ctagttttcc aaattgctta tatgattcta
     1741 tttaaataat agaagagagg ggttgtgaca gaataattgt cgcaacctct tctaacattg
     1801 tagtggaaca ctattttttt ataagaatat attaagtttg atctgttatg ttatttaaac
     1861 tattgtttcc tttatttacg ctgttcaagg aaaatggtat gataaaagat aaccatatgt
     1921 taaatcaatc ggacggagga acaacagatg aaagtactag taacaggttt tgatccattt
     1981 ggtggggaca aagtgaatcc agcctatgaa gcagtaaaaa aaatgccaga tgaaatcagc
     2041 ggtgctgaaa tcatcaaagt ggaagtaccg accgtattcg aaaaaagcag tcaagtagtc
     2101 aaagaagcca tccaacagca ccaaccagat gtcgtgatct gtgtcggaca agcaggtggg
     2161 cggtcagcag tctcgttcga acgtgtcgca atcaatctag cagaagcaag aatccctgat
     2221 aatgaaggaa atcagccatt cgatacggct ttagaagaaa acggtccagc cgcttacttt
     2281 acctcattgc caatcaaagc aatgacaaaa aatgtacaag atcatggtct accagcgtac
     2341 atctcttata cagcaggaac atttgtctgc aatgatatca tgtatcgttt gcttcatttg
     2401 atcgaaacac aattcccggc aattcgaggt gggttcatcc acgtgccatt ctcacccgat
     2461 caagtgatcg atcgtccagt gggtacgcct tctatgtctt tagaagacat tgcttcttct
     2521 ttgacttatg cagttgaagc agcagtagaa aataaagaag atatttccgg aaatgccgga
     2581 acaactcact aatttagcaa gaaacactgg ctggttgcta acgttatgtt agtagccagc
     2641 tttttgtaat tagaaaggaa agaaacagcc gtggaagaat ggcaatccgt ctttgaagaa
     2701 tggtttccaa aagaaatcag caaaagctat cccatcaaaa tttctaaaca atacacaagc
     2761 agtcaacgtt gggagatcta tgcaaaatta acgaaaaaac agcgggaact agtcgataag
     2821 caccgccgat atctaatcag ctctcgtttt atggaagaac attatttagc tgcaacagat
     2881 tgggtgtttt ctgacttcaa gatcaatccg ttttttcgaa caaaacgaag ccagcaaaaa
     2941 ttgtattgtg agtgtggtag agaattaaaa gtgcaataca tcgttaaatc gccaaaaacc
     3001 ggaaagatcc taaaactagg catcaaccac tttgccgatc acctgcatgt ctctccgaca
     3061 gtggcagctt cgatccatca gggaatgaca aaagttgatc tggccttaga cgaattatta
     3121 tggcttaagc agaagaatat cgattttcct gaaggactat ggcaaaaata ctgttttgtc
     3181 ttgtaccaaa atcgtcggat gaaacagcca tatttaccgg atataaaatt agctcagcgg
     3241 ctcgcagaat ttcgccaagt agaaatgccg atttacatag cggattatca agcattggaa
     3301 aatgaaatca agaaaataag tgaacacatc aatgggcagt caaaaaagcg acagataaaa
     3361 aaagaactat ttgacgactt tgcagaagaa ttagtaaagg atgtagaaga attcttgatc
     3421 aactatcgcg catttttacg taaagattgg caatccatcg tatatgaaga agtgccagta
     3481 catcctaatg cttattttga aacatttatt tccgtgttga gaaagacaaa acgtcaacga
     3541 acaccagaag tcactgctca gatggaatat tttgcgaaaa accaacgatt catccaacca
     3601 aaaatctatc tatttatttg gaagcaatat tgtcgctatg gttttacaga agggttcttc
     3661 gacagcattc ctcggatagt cagaaatggt tttttaaaag tattgcgtaa agaacgagaa
     3721 gctatacaat ctgctgataa gaaagaccgt acagtctcaa aagaaaaatg gcaactagta
     3781 gtgaaagata tccaatctgg aaatgtgcaa gagacaatcg acaaatggaa aggaaaacat
     3841 tatcgtttta ctgaagctca gaaacaagcg ttggaatatt atcagaagtt agaagaatca
     3901 ctgcgtttca atgatgaggc aagaaagtat ctgaaggaat tactataaaa aacatactaa
     3961 gattagaggg gaacatcatt gacgaatgat gttctcctct attttctatt tttagattat
     4021 agtgaagagt gaaagaaagt tcgaacaaag ccttgaggac tacgagtccc tatcaaaaac
     4081 cgaacttctt tactgtcatt ttagaatcag gtttacgtat gttggggcca tgagcccgtg
     4141 tataggaaat tggaatcgaa atgaagcagt aaagttcttt caaaacttaa aaaggaggaa
     4201 aacaaatgcc ttacattctt gcattagatg tttcaatggg acacagctac agtgtcctat
     4261 acaaagacga cacctgctta tttgaagacg agattgaaca cactcaaaga ggatttcatg
     4321 cattactcga agagatccag cacttgcctg agcgtccatt gattgttttt gaagcaacgg
     4381 ggatttattc cagaccagtc gaaaagttct ttcaagatca tcacttttct tactgtttgt
     4441 taaacccgtt agaggcgaaa aaacagatgg aagaaagcac cttacgcagt tggaaaacag
     4501 ataaatcgga tgcccaccgg ctcgcacaga ctcatcttaa aaatcaacgt cagccaaaag
     4561 aagtacaaag caatttttac ttagagatgc gcgatttagc ccgtttttat caagaaattg
     4621 agaagaaaat cactcgtctg cgcatggatt tacacaactg ccttcaattg acttttccag
     4681 agcttgaaca gttcttttca aacaggctga ccccttacgc attgacattg atccgtcttt
     4741 ttccccatcc agattttgta ttggcatcta ctcggacaaa aataaaaaat aaactgatca
     4801 acgaaacacg gaaaaaaatc tccgcgaatc gtgccgagca aaaggcagat caaatcatcc
     4861 actatgcaca gtgtgcctat ccagctgttg aaaaagacag tatccactgt caaaaaacga
     4921 tctattacgc cgaacttctc caagatttac tcgaacaaaa a
//
LOCUS       DAZUMT010000150         4956 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000150 DAZUMT010000000
VERSION     DAZUMT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.154"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..828)
                     /locus_tag="ACG8FM_002732"
     CDS             complement(55..828)
                     /locus_tag="ACG8FM_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5095321.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            972..1322
                     /locus_tag="ACG8FM_002733"
     CDS             972..1322
                     /locus_tag="ACG8FM_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5095322.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1303..2019
                     /locus_tag="ACG8FM_002734"
     CDS             1303..2019
                     /locus_tag="ACG8FM_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5095323.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2019..3467
                     /locus_tag="ACG8FM_002735"
     CDS             2019..3467
                     /locus_tag="ACG8FM_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5095324.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3537..4046)
                     /locus_tag="ACG8FM_002736"
     CDS             complement(3537..4046)
                     /locus_tag="ACG8FM_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095325.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4036..4761)
                     /locus_tag="ACG8FM_002737"
     CDS             complement(4036..4761)
                     /locus_tag="ACG8FM_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095326.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1703 a          843 c          998 g         1412 t
ORIGIN      
        1 ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat
       61 cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct ttgtgataaa
      121 ggtagaaagt tcttttaatc cgtcataggc gttgtccata gcgattttgt tgtcgcaagc
      181 ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa ctttttccag
      241 ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt tcttcactgt
      301 atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt cgtgatagta
      361 ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt taaagcctaa
      421 aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct
      481 atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac cgttcaactg
      541 tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat agatttcttc
      601 gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc
      661 catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga gaacattgtt
      721 ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt tcgcatctag
      781 taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa ataaaactcc
      841 ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa
      901 gaattcctga acatattggt tcagtcaaca atttagagta tactataaga agagaattta
      961 ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt
     1021 ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg
     1081 caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta
     1141 tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt
     1201 ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa
     1261 agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt
     1321 gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa
     1381 gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat
     1441 tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga
     1501 gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa
     1561 agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt
     1621 cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca
     1681 agagaagaag cagcagtttc agatgatcat tttgatatcg agactagtca tttcaagatg
     1741 aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa
     1801 gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag
     1861 ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat
     1921 atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg
     1981 ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac
     2041 tcttagcttt ttgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac
     2101 aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat
     2161 cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg
     2221 cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta
     2281 tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta
     2341 ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga
     2401 ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga
     2461 atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg
     2521 ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta
     2581 ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga
     2641 cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat
     2701 ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag
     2761 aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga
     2821 gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg
     2881 aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt
     2941 tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta
     3001 aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag
     3061 aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt
     3121 atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa
     3181 acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag
     3241 atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag
     3301 tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc
     3361 agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa
     3421 caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta
     3481 taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag
     3541 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     3601 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     3661 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     3721 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     3781 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     3841 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     3901 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3961 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     4021 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     4081 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     4141 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     4201 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     4261 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     4321 aactgactgg aagctgacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     4381 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggcgcca
     4441 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     4501 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     4561 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     4621 attggctaaa gccgattgcg aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     4681 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt
     4741 agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga
     4801 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
     4861 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
     4921 tgtttattcg aaaagaggag cagcgacaat aatttt
//
LOCUS       DAZUMT010000151         4932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000151 DAZUMT010000000
VERSION     DAZUMT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.155"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8FM_002738"
     CDS             85..972
                     /locus_tag="ACG8FM_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5095327.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8FM_002739"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8FM_002739"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS5095328.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8FM_002740"
     CDS             2274..2720
                     /locus_tag="ACG8FM_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5095329.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8FM_002741"
     CDS             2993..4345
                     /locus_tag="ACG8FM_002741"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS5095330.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8FM_002742"
     CDS             4516..4803
                     /locus_tag="ACG8FM_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5095331.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1394 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tt
//
LOCUS       DAZUMT010000152         4715 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000152 DAZUMT010000000
VERSION     DAZUMT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.157"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..506
                     /locus_tag="ACG8FM_002743"
     CDS             <1..506
                     /locus_tag="ACG8FM_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS5095332.1"
                     /translation="ENIEKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLK
                     TDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNI
                     INANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKW
                     IQKQIAY"
     gene            479..1156
                     /locus_tag="ACG8FM_002744"
     CDS             479..1156
                     /locus_tag="ACG8FM_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS5095333.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1107..2288
                     /locus_tag="ACG8FM_002745"
     CDS             1107..2288
                     /locus_tag="ACG8FM_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS5095334.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2301..3278
                     /locus_tag="ACG8FM_002746"
     CDS             2301..3278
                     /locus_tag="ACG8FM_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5095335.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3278..4186
                     /locus_tag="ACG8FM_002747"
     CDS             3278..4186
                     /locus_tag="ACG8FM_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFS5095336.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            4187..>4715
                     /locus_tag="ACG8FM_002748"
     CDS             4187..>4715
                     /locus_tag="ACG8FM_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS5095337.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTP"
BASE COUNT         1783 a          574 c          871 g         1487 t
ORIGIN      
        1 tagaaaatat agaaaaacaa attttagctt tgatcaattc aatggaacaa caatctcaac
       61 caattatttg cattttacct aatagtgatg ctggtagcga aattatcaga aaaatgtacc
      121 agaaatattt aaaaacggat attatttata ttacgaattt agagccggag gaatacctga
      181 ctatattaaa agagtgcaaa tttatgatcg gaaattcttc ttctgggata attgaagctg
      241 catcattcaa aataccagta ataaatttag gaaccagaca aaatggacga gaacaatcta
      301 aaaatattat aaatgcaaat ctaaatgaaa atgaaattga aactgctttg aataaaatac
      361 aagatcctca gtttataaaa agcttgaaaa atgttaagaa catctacggt gacggcaaca
      421 gttctagaag aataacaaaa acattgaatc aaatattgaa tgatggggaa gatattaagt
      481 ggattcaaaa acaaatagct tattaaaata tactagtgat ccatacatta ttattggggc
      541 tggcggacac gcaaaagtca ttattgaaac cttaagatta gaaaataagt taatatatgg
      601 attttttgat gataagcaaa ataaaatgca agatctgaaa tatcttggga aaataaacga
      661 attgaatagc tgggcggata gcaaaaaaaa aatctataaa tttattgtag ctattggtga
      721 ctgtgtagta agaaaaaaga ttataaaaaa attgaatatg ccaagtgagc aatatggaat
      781 agctgttcat ccaagtgcta tagtggcaaa agaaatttca attggttatg gaactgttat
      841 ttttgcgaat gctgtaatta acccagatgc tgtaattgga gagcatgcta taataaacac
      901 aggtagcatt gttgagcatg attgtagaat taataactat gttcatttat ctccaggtgt
      961 atgcttagcg ggtggagtac atgtaggtgt aggaactcaa ataggaattg gttctcaatg
     1021 tatccagatg aagaaaattg gatcttggtg tatgataggc gcagggagca ctattgttaa
     1081 ggatattccc tcacattcct tagcttatgg caatccagct aaaattaaga aagaaggaat
     1141 taattttgaa ttctagaatt ttattatcaa gtcctcatat gggaggaaca gaagaaaaat
     1201 atgttgctaa ggcttttaaa gataattgga tagcgccatt aggtgaaaat gtggataaat
     1261 ttgaagaaag tattacaaaa tatactggtt ctaagggcgg agcttctgcg ttaagttcag
     1321 gtacagctgc catacatctt gccttaagac tgcttaatgt tggaaaaaac gatattgtct
     1381 ttgctccttc atttactttt atagctacag taaatcctat tttatatcaa ggggctgtac
     1441 cagtgttcat tgactcagaa cctaatacat ggaatatgag ccctgttgca ctagagagcg
     1501 cattagaaga agctgaaaga aacaaccaat tacctaaggc aatcatcgta gtgaatttat
     1561 atggtcaaat ggctgattat gaacgaattt tagctattgc aaaccaattt aatgttccag
     1621 tgatagaaga tgcagcggaa tcgttaggtt ctacttataa gggaatttcg tcaggcttat
     1681 ttgggacaat tgggatctat tcatttaacg gtaataagat catcacaacc tcaggtggag
     1741 gagctctagt gtctagtgat aaaaaatatg ttgagcattc aagattttta gcaacacaag
     1801 caagagatca agcaccgtat tatcaacata gcgaaatagg ttataattac cgtctaagca
     1861 atatttctgc cggtatcgga cgtggacaaa tggaagtatt gaatgataga gtagctaaac
     1921 gaagagaaat tttcaatcga tataaggaag ctttttctgg aattgataga attgaaatga
     1981 tgccagaagt ggatggcatg gtgccaaatc gttggttgtc tactttaacg atcaatgatt
     2041 ataattctat tgatcaagta gttaacgtaa taaataaact aaatgaaaaa aacattgaaa
     2101 caagagcttt gtggaaacca ctacatttgc aaccagtatt tcaaggggta aaaatgtatt
     2161 accatgataa taatcttagt gtgtcagaaa agttatttaa tagcggctta tgcttacctt
     2221 ctggaagtaa tatgacagat agtgaacaag aatttgttat tgatgagttt aaaaaaatat
     2281 tcaagtaggt gtgatatttt atgaacatag gaattattgg tttaggttcc atggggaaaa
     2341 gaagacttag actcattcaa gaacacttta atgatatttt actatgtggt gttgatacta
     2401 atgaggcgag acgaaaggaa gttgaaaagg aatttggaat accaacttat aatagtctaa
     2461 cagaggcaaa aaatttgttt gaaataaatt cagtttttgt ttgtacatcc cctataaccc
     2521 atgaatttat tataaaagag gctttaaatt tagattgtaa tatctttacg gaaatcaatt
     2581 tattaaatga atattatgat gaaataatcg aagtagctaa aaagaaagat agattacttt
     2641 atctatcttc tacatttttg aagagaaaag aaattcaata tattaagtct aaagtgacgc
     2701 ataataaaaa aatttcttat caataccatg taggacagta tttacccgac tggcatcctt
     2761 gggaagatta tcgcaatttt tttgtttcaa acaaaaaaac taatgggtgt agagaaatat
     2821 tcgctattga attaccttgg attatagata catttgggaa agttaaatcg tttactgtaa
     2881 tgacccagaa attatccaat cttgatattg attattcgga cagtttcact cttattattg
     2941 agcatgaaac tggtatagtt ggaactttaa gtgtagatgt tgtttcaaga gtagctaaaa
     3001 gaaatttaac tataattggt gaaaagattt taatagaatg ggaaggtaac ccggattctc
     3061 tattttcatg gaatgaagaa acttctagta tgacagatat tcaattgtac gatgaggtta
     3121 tgcatgataa taattattca catactatta ttgaagatgc atatttagag gagattaaag
     3181 aatttttcac gcttttggaa aagaaaatgg aaccccaata tacttttgaa aaagataagt
     3241 atattattaa cttaattaac gacattgagg taaattaatg atgaaagtaa ttttttttgg
     3301 gttagggtca attggaaaga gacatctaaa aaatctaata agctatggaa aaaagaattc
     3361 tattaatttt gatataacag cttatagtag cagtggaaaa aaagagtttg aagaaataac
     3421 gtatattcaa aatagagacg atttggatac acattatgat atagctttta ttacaaatcc
     3481 gacttttaag catctggaaa cactaaatgt aattgttgaa aaagttgatt acatattttt
     3541 agaaaaacct gctttttctg agagtattga tcttactcaa tataattatg attacaaaaa
     3601 tatttatgtt gctgctccac tgaggtataa aaatatcatg gaagttgttc gtaatagtat
     3661 tcaaagtaaa aaagtatatt ctgtaagagc tatttgttct acttatcttc ctgattggag
     3721 aaaaggtact gattatagaa ataattattc tgcctttaaa tctatggggg gaggagtaga
     3781 attagactgt atacatgaat tggattatat tataaaccta tttggtttcc ctaaatcagt
     3841 aaaaaaacag tttggtaaat attcgaatct tgaaattaac agtaatgatt tagcaacata
     3901 tatattgaga tacgatgata aaattataga aattcattta gattattttg gaagagttcc
     3961 tacaagaaaa tttcaattgt ttactttaga tgaatgtatc aatgttgact tattaaataa
     4021 taaaataagt tttgaagaaa acattgttaa agaaatgcct gaatctgcta atgatatgta
     4081 tgagaaggag ttgaaatatt tctttgaaaa cgtagtaaca ggcaaagaaa actggaatga
     4141 tttatatcat gcaaacgaag tattgaaaat agctgaggag aaataaatgg aaattttatt
     4201 tactatttgt ggtagagctg ggtctaaggg gtttaaaaat aaaaatttga aatatttggc
     4261 aggaaagccc ctagtccatt tcgctttagc aatgatagat atctatcaac aaaaacatcc
     4321 tgaacataat tgcacgactt gtttgaatac ggacagtaat gagttagtag aattgacgac
     4381 taagttaaat ccgtctgtga aaattgtaga acgtaaacct gaattagcta cagatactgt
     4441 agggaaaatt gaagtgatac gtgattcatt cttacaacta gaaagagaag ataatatatt
     4501 tgatttaatt atagatttag atttgacttc acctttacgt gaagtttcta atcttgaaga
     4561 attaatagat gaacatctaa aaaatttaga taaagacgtc tacttttcag ttgtttctgc
     4621 aagacgtaat ccctatttta atatggtaaa aagaattgaa aatgaagtta ctatagttaa
     4681 taaatcaaga tacacatcta ggcaacagac acctg
//
LOCUS       DAZUMT010000153         4589 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000153 DAZUMT010000000
VERSION     DAZUMT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.159"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACG8FM_002749"
     CDS             186..2855
                     /locus_tag="ACG8FM_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS5095338.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACG8FM_002750"
     CDS             complement(2946..3560)
                     /locus_tag="ACG8FM_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5095339.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3679..4218
                     /locus_tag="ACG8FM_002751"
     CDS             3679..4218
                     /locus_tag="ACG8FM_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095340.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4215..4553
                     /locus_tag="ACG8FM_002752"
     CDS             4215..4553
                     /locus_tag="ACG8FM_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFS5095341.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa ataacggtgt
     3601 acatttttaa aaggagatgt ataatcgaga tttagaaagc gttttcctca aaatttaatg
     3661 aattaggaag tgattactat gaaaaaatta tttagcgcac tttttattct gttatttggc
     3721 tttattggta ttgggacttc tggaaatata gttaaagcta gcacgatggt tgaacaaaca
     3781 agtgaaatgg caaaaacttt tagtcaatta acaccagaag aacaaagtta cttcaaatcc
     3841 aaaggattta gtgataaaga tactttttac actcaaaaga tagagcctca agatccgaaa
     3901 acacgttttt ctgcaaatgt attgcggtta accgcatcta ctaaaaaaat tactaatacg
     3961 caggggcgga cagaatatat tataacaagt cccaattcgg cgatgtatcg tatttctaca
     4021 cgcttgagtt taggtagtgc aaaatcatat actcaaacag taaatctagc taattcttat
     4081 ggttatggtg gaggaatgta ttttacattt actgggaaaa gacaatatta cagtgtaaaa
     4141 ttagctatgc aagtttataa tacatttggt gtagggtcag tctcttgcac tgcgggagga
     4201 ttgacaatag gaaaatgata atttcttact atgtcttaaa tagaagtgat gatgaaggac
     4261 tcttcgtttt tcaatcatta catgaggata cgatgctaaa agagttttat aatcggatta
     4321 agaataaaca gatagaaaca aatttatatt ttttaagtga ctctatcttg ccttttatta
     4381 gcaaccctaa aattcttaac ttgttgcaga caaaagattt gaaaagttta ccgattattg
     4441 tcattgatga ggaaattgta aaatatggcg cttatctaag cgtagaagaa ttagaaaaat
     4501 taactaacat tggaatgagt attcaggagg ataatggggt acatggaaca tagaatgaat
     4561 atgcacagtt gagaacggaa tttgacatt
//
LOCUS       DAZUMT010000154         4566 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000154 DAZUMT010000000
VERSION     DAZUMT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.160"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8FM_002753"
     CDS             complement(<1..300)
                     /locus_tag="ACG8FM_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS5095342.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8FM_002754"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8FM_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8FM_002755"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8FM_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095343.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG8FM_002756"
     CDS             1706..1942
                     /locus_tag="ACG8FM_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095344.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG8FM_002757"
     CDS             1949..3901
                     /locus_tag="ACG8FM_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5095345.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8FM_002758"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8FM_002758"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5095346.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1562 a          803 c          755 g         1446 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaat
//
LOCUS       DAZUMT010000155         4486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000155 DAZUMT010000000
VERSION     DAZUMT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.161"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8FM_002759"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8FM_002759"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS5095347.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACG8FM_002760"
     CDS             complement(1036..1956)
                     /locus_tag="ACG8FM_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5095348.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACG8FM_002761"
     CDS             2069..2821
                     /locus_tag="ACG8FM_002761"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS5095349.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="ACG8FM_002762"
     CDS             3685..4218
                     /locus_tag="ACG8FM_002762"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS5095350.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1323 a          878 c          795 g         1490 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttacc
//
LOCUS       DAZUMT010000156         4433 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000156 DAZUMT010000000
VERSION     DAZUMT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.162"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG8FM_002763"
     CDS             complement(543..2048)
                     /locus_tag="ACG8FM_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5095351.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG8FM_002764"
     CDS             complement(2045..2731)
                     /locus_tag="ACG8FM_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5095352.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8FM_002765"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8FM_002765"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS5095353.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAZUMT010000157         4418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000157 DAZUMT010000000
VERSION     DAZUMT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.163"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACG8FM_002766"
     CDS             59..913
                     /locus_tag="ACG8FM_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5095354.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACG8FM_002767"
     CDS             1010..3259
                     /locus_tag="ACG8FM_002767"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5095355.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4418
                     /locus_tag="ACG8FM_002768"
     CDS             3262..>4418
                     /locus_tag="ACG8FM_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5095356.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1556 a          625 c          807 g         1430 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagt
//
LOCUS       DAZUMT010000158         4332 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000158 DAZUMT010000000
VERSION     DAZUMT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.164"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8FM_002769"
     CDS             127..579
                     /locus_tag="ACG8FM_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095357.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8FM_002770"
     CDS             593..1282
                     /locus_tag="ACG8FM_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095358.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG8FM_002771"
     CDS             1407..3758
                     /locus_tag="ACG8FM_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS5095359.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG8FM_002772"
     CDS             3838..4233
                     /locus_tag="ACG8FM_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095360.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1525 a          548 c          867 g         1392 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt tt
//
LOCUS       DAZUMT010000159         4226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000159 DAZUMT010000000
VERSION     DAZUMT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.165"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..462
                     /locus_tag="ACG8FM_002773"
     CDS             25..462
                     /locus_tag="ACG8FM_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095361.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            557..1441
                     /locus_tag="ACG8FM_002774"
     CDS             557..1441
                     /locus_tag="ACG8FM_002774"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5095362.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1663..>4226
                     /locus_tag="ACG8FM_002775"
     CDS             1663..>4226
                     /locus_tag="ACG8FM_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS5095363.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
BASE COUNT         1378 a          712 c          957 g         1179 t
ORIGIN      
        1 aaaaatgcac gtagaagtag ataaatggag tggttttgca aatttctttt atttctagaa
       61 gttattcacg tatacggatt tgtttttctc acaaataaaa aggcaggaag tacaatggat
      121 catttgtata taaaatatat tcatcaattt tccgttttgg tattaggcac actaattata
      181 gcggggattg ttgcatttat tattcaaggt cgtgacatat taacagtaca tcaagagttt
      241 ttcagttatg catttggtat tcctctgatc gctataaggt ctgataacaa aaagtggaag
      301 aaacgctatt acattctctt gctatttttg tgtttttatc tgccacctaa gatttttact
      361 ttattaggta tacaatgcct atcattttct tttatttcct atatcattgt tttaattttc
      421 ctcagtttca tagtatgggt attttatctt gtcaaaaaat aaatgaatgt ttcatgaaca
      481 gtacttcatc acagcctaat ttttttcttt atgctatcgt tatattaaag aaatcaatca
      541 gaagggaaag aaagtgatga aaaaagtttt attagtggta aaccccagtt caggcggtga
      601 acaagcaaag gaatttgagc aattagcgat agcaaaattg gaatcggtgt ttgatgaagt
      661 ggtcgtcttg catacaaaaa aagcagggga tgcaaaaaac tttactcgcg aagcggctac
      721 ggaagggtat catagtgtgt ttgtcatggg cggagatgga acagtcaacg aaggaatcag
      781 tggaatcgct gaacaagaac atcggccaaa tttcggattt ttcccattgg gtacggtgaa
      841 tgacttggct cgagcattag ggataccgct agaacctgag gaagcgatca atcatttttc
      901 gattgaatca gtcaagccat tggatattgg aaaaataaat gacgactatt ttatgaatgt
      961 cgtcgcaatt ggctctatcc ctgaagcaat caatgatgta gatgcggaga agaagacgaa
     1021 atttggaaaa ctagcttatt tcatgtcagg aatcaaacag ttagcttcca cacaatccta
     1081 ctcgtttcac gtagaagttg atggaaagaa ggaagaaatt gagagcagta tcttgctgat
     1141 aggactaact aactcagtag gcggttttga aacattactt ccaaatgcaa aagtggatga
     1201 tggtaagctc catttggttt atttaaaaga ttcttctttg atcgatacgc ttaaaacatt
     1261 gccagaccta ttaaaaggag tagatgaatc tacgaaaaat ctagcctatc ttacttgtga
     1321 gcaattagtt gtgtcattag ctgatgagga agaattgacg acgaatgttg atggagatga
     1381 aggggataaa ttacctgtga caatcaaagt gttaccctca catttacagg tttattgtta
     1441 aaatggaggt tgtgaccgaa gagttgtcag gaggacatcg gttgttcgga ataagggact
     1501 gtgacaagac ttttgtcaca gccttttttt gtacaaaaaa gaaattttca cctaacagaa
     1561 aaatttgcca taaaaataca tattttaaat atgaaagtac aattacaaat aaattaaaac
     1621 gttaaaataa aattgtcaaa acgtcagaga ggatggtgga gaatgaatca atatgtaaaa
     1681 aagctgattg cgtcgatttt gtttgtcttc cctctcttga tggtgacatc cgtttttgcc
     1741 gatgtagaga aaacaagcga atcaacgcaa tcagagaatc aaagttttga caagactact
     1801 ttttcagaag aatcaattac ttctgaaact acagaatccc aaagtgaggc gcctacttct
     1861 aatacaaggc aagaagaaaa taagacaagt gagacctcag aagtagctga acaaacgtct
     1921 gatagcagtg aagaggtaca ggttagagtt gtttcagcgg atgggtacag cgaagctgcc
     1981 acaacagaag aactgacgca gcttctcgca gacgaatcag tagctaagat tcgtttaact
     2041 cagcctttga ctttagacag ggaattggag atcaagcgag atatcgttat cgattttggc
     2101 ggacttgctc ataatttcgg cacacatcac atctatatca atgagacacc tcagtcgatc
     2161 gagtttcaga attttaaagg aacggcagct catccaggtg ggttgatacc aaatatcgat
     2221 ggaaatgcca ttatcattgc ttatatgtcc gatttttggg gaaacagata tcattttacg
     2281 ggagaaataa aatttaccgg gacacttgac ttatatgatg gttctaaatt aggcctgatt
     2341 tacgcccctc gtgcaactgt cacattagat ggtgtttccg gtgtattgga tgtccaacca
     2401 gtgaaagaag ggatgaatgc cgctccgggt aaagcttatt tttgtagaag ctatcagcta
     2461 aatgtaatca atggtagtca gctttatgga ccatatctag gaaaattcta tggttttcta
     2521 gattcaggtg atgagacggg cagtggaaaa acagcaccag gactccatat cttgagtggt
     2581 tcaaaggtct ctcttgacta tgatcgggca gatggcgtca gcgatgaagg agaggctgtc
     2641 gatacgctac ccgggaatgt cacatttaaa gtcagtggct caggaagtga attttcggta
     2701 aatacaaaga tcaatattac caacgacaac aatcgcggaa ttatccaaat gagaggctca
     2761 ggatcaagag tcgctgtgtc gaatgatggg aaaatcacaa tcaatacagc gactactggc
     2821 ggatttcgcc tgcaaggaga ttctagtcaa atccatgtat caagtggtgg acaaatctcg
     2881 gtcaaaatgg ctggagatgg cgaccaacct ggaaacaatg ggatgcgttt tgtagggaaa
     2941 ggactttctt tgatcgtaga aggagccgga tcagtcatcg atattgataa gcaaagtggt
     3001 cgttctagcg cagttcgttt tgagaacggt acgcaaaaac taacagtaac taatggaggc
     3061 tcattgaagg taagaaatgc aggggatggc aaatcttatg tgaacagact caatcaagga
     3121 aaccaagcga tccaatttta tgacgcttct ggttttctag aaagtccagg gtcagctgat
     3181 tttacggtta ctggagaaaa atcaaatatc gatatacgcg cagattccgg agctaccgta
     3241 gatacaactg gagatattga tttgaacttt atggctggcg aaaaaactta tttagtcatg
     3301 gtcgggaaaa ctggggaaga taagactggc atcatcagtg gggatgtgtt gaatgtagac
     3361 ttagaatctc ctacctattt cgatttccaa aacaaacgga tcggaagcga gactaatggc
     3421 ataggtggct gggtatttca aggaaatggc gattcttctt tgaagatcgc tcattcagaa
     3481 attgctttat ggcgaaaggc aggagtaacg ttcgataact ttgaaaaaga tccggattat
     3541 ttttctgata tgacagactt atcgatcaat ggaaccgact taggtaattt tgtatcaagc
     3601 cctagtgcag gtttaacaaa tgaatttgct cagacaggtg ccggaatgaa aaattataat
     3661 cgaatcagtg ccaataacca gcttccgatg attgaatcct taagagtacc aacgaatgca
     3721 gataaaaaaa tctatgggca tgtcgttgtt cccgaaggtg tcgaggcaaa gccaagagat
     3781 gcttggacag gcgaagtaga agtgacttta cgtattactt atgcagatac gacgaaatca
     3841 cctgtagaaa ttactgctct cacacaagga caaacagatg aaaatgaagg gacaggatat
     3901 aatccgtggg gagaaggcga acaaggcggt ttgtttgagg ttcaagtacc caacgaagaa
     3961 agactaacaa cgggggatca agtgcaaata gttgctgcta aaaaagtaag aggcggccaa
     4021 gaagtgacag aactggactc tgccaaaacg acagtcgatg tgattcctcc tgagcctgct
     4081 aaattaagta ccacattgat caatgcagct tctcgctcaa tcacaggtac aggcgaagca
     4141 ggagctttgg tttcattgaa aaaaaatcaa gctgttttgt ccgaaacaac tattgatgat
     4201 acaggtaaat ttgttctttc tatccc
//
LOCUS       DAZUMT010000160         4190 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000160 DAZUMT010000000
VERSION     DAZUMT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.166"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1155)
                     /locus_tag="ACG8FM_002776"
     CDS             complement(1..1155)
                     /locus_tag="ACG8FM_002776"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS5095364.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1340..3781
                     /locus_tag="ACG8FM_002777"
     CDS             1340..3781
                     /locus_tag="ACG8FM_002777"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS5095365.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(3938..>4190)
                     /locus_tag="ACG8FM_002778"
     CDS             complement(3938..>4190)
                     /locus_tag="ACG8FM_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095366.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         1323 a          783 c          917 g         1167 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagctgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt
     1201 atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa
     1261 tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt
     1321 tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat
     1381 aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac
     1441 taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg
     1501 aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg
     1561 tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc
     1621 agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg
     1681 aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata
     1741 tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca
     1801 tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga
     1861 tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc
     1921 tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt
     1981 tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg
     2041 aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc
     2101 atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc
     2161 ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt
     2221 gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt
     2281 tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat
     2341 atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac
     2401 tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat
     2461 tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga
     2521 tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga
     2581 aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga
     2641 gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc
     2701 gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc
     2761 tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc
     2821 tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat
     2881 caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc
     2941 aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt
     3001 ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat
     3061 gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga
     3121 gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga
     3181 aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat
     3241 ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg
     3301 gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag
     3361 cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca
     3421 gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg
     3481 cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc
     3541 agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt
     3601 gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt
     3661 agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga
     3721 agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc gggaaaaata
     3781 aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt tactgcagat
     3841 cttgcttttt tggctcttcg tcaaattgtg ttggcgaaga caaatttgaa taaaacttag
     3901 tccgatatgc ggcgcgttct gtgcgccgca tattttttta gttttcaaag atcagacctt
     3961 gttgtaaata gatccgtgcc cgaaagttat aaaagttgcg ataaccaaac gcaattcgtt
     4021 taatcagctt gatcttgtta ttcagcccct cagtgacgcc gtttgagtgg ggcaccttaa
     4081 aggcatttga aatacccgat tggtagcgat ggaagatcgt gaatttcttt ttaaacgact
     4141 gaggtaacgt agatgaaatt gttttaaggg tttcttgaaa gccttccgta
//
LOCUS       DAZUMT010000161         4069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000161 DAZUMT010000000
VERSION     DAZUMT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.168"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACG8FM_002779"
     CDS             85..1434
                     /locus_tag="ACG8FM_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5095367.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACG8FM_002780"
     CDS             complement(1562..2152)
                     /locus_tag="ACG8FM_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5095368.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG8FM_002781"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG8FM_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5095369.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1175 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DAZUMT010000162         4059 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000162 DAZUMT010000000
VERSION     DAZUMT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.169"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            349..657
                     /locus_tag="ACG8FM_002782"
     CDS             349..657
                     /locus_tag="ACG8FM_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS5095370.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            684..983
                     /locus_tag="ACG8FM_002783"
     CDS             684..983
                     /locus_tag="ACG8FM_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFS5095371.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            1157..1702
                     /locus_tag="ACG8FM_002784"
     CDS             1157..1702
                     /locus_tag="ACG8FM_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFS5095372.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLSDHSLLFQ"
     gene            1858..2154
                     /locus_tag="ACG8FM_002785"
     CDS             1858..2154
                     /locus_tag="ACG8FM_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095373.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            2147..3037
                     /locus_tag="ACG8FM_002786"
     CDS             2147..3037
                     /locus_tag="ACG8FM_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFS5095374.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            3119..>4059
                     /locus_tag="ACG8FM_002787"
     CDS             3119..>4059
                     /locus_tag="ACG8FM_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5095375.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFTDIINGQT
                     PQTPVVEESTQQATTTTTQSTSSSGISNWEAAKETVLNNKALWIYESPDKNEANTNII
                     ESTQYRKDLMQDENGEFYPVDISTDDIGNTVMNGGKQ"
BASE COUNT         1508 a          583 c          782 g         1186 t
ORIGIN      
        1 ttatagttaa agctttcaat tttgacttcc tttcttgtat tatttataat aacaatagta
       61 gtataacatt aataggatag ttcattaagc aaaattaagt atcttagtta ctaataacta
      121 tataaatttt aaacttgtag tcgcaattta gtatagaggt actaaatatg aaaactccat
      181 acttttagaa aagcatttct tatatttagt tgtgatgtct attcagttaa ttgaaataaa
      241 taatatagca atgaaataga gataaattca aatatatatt atatccatga agtccaatct
      301 tctttataga agattgggct attttttacg acaaaggagg gaaaagaaat gtttcaaaat
      361 aaaggacaac gttatgtcac aaagggagta atggacagtt tactggtgga attacaaact
      421 ttatgttgga atttaatcga tcaaaacgtt caaaagaagc ttcctttaga ctatcttcaa
      481 atctttgaat tttcgataga aaaaggaaat caaaagttgg ttcatcgtca agaagaaccg
      541 gaagcaagaa aagagtatct gatttcacct aagcttcgtt tggaatcagt gtctcaaaaa
      601 atatgggtca ttgattcggg tgagtatcaa acgatgttat taccagaaga atactgatat
      661 ctaaaagaaa ggagcgaaat gacttgttag aaacggctat cgaagtactg gaaaagtgtg
      721 cacagctagt cactactcct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg
      781 aaattgaaat gggtacgctt cctaaaaatg ttcatctacc acgtttagtg atgacacatc
      841 tttatattta ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata
      901 ttaccagcca gcgagaattt gtttgtgggt tactggtaga aggacgattg gtttggagtc
      961 agattggagg aacgaatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa
     1021 gatatcctta ccgcgattta tcgaattctg aagaaaaaag gagaaagaat atgaaatatt
     1081 ttcaactagt attgacactt ataacggcaa tggtaattga attgcttgaa caaaaattag
     1141 cataggagaa aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg
     1201 agatcctcat catacccatc gctatgtttt caaacgacaa tgggcaatca aaggaaaaga
     1261 agagaaagaa aaattagcag tagtcattac cattcgtcca acagatacag aaccatttac
     1321 gaatgatttg agtatgttat taatcgaaaa gaatatccga caattgggat ttactggttt
     1381 tattgccgtg aatctttttt cgtatacaaa agcgaaaagt cctcgggtat tttctcgtgg
     1441 caatgatgaa caatctttgg aaatattaac gaccattttt acagaaaaga aaatcaacac
     1501 tattattttt gcttgtggtt cgattacggc aacgagtcaa gtggcttatg aacaagcaaa
     1561 gaccatttac gatcaattga gtgcgaaaca aaagaagatg accaaagtat tgataaatgc
     1621 agaaggcaag ttatctcacc cattaagtat tcatgttcgt aatgaatggc aattgtcgga
     1681 tcattctttg ttatttcaat aaacgaaaat aagtattcaa tgtatatgtt tctttttctg
     1741 ttattttact tgttcttttg cagactaaac aacttgtaaa acctcttgtt atatgcatta
     1801 tcaatgaagg aatcaatatg atatttttaa aatgtcaggg gaaatggagg tgagcaagtg
     1861 aaagtagatg gggaatgttt gacgagttcg tataaaagta tctatgaatt ttttgaggaa
     1921 aaaggactag acacggattt actttatcag ttttatatcg cttttcgtgg gcaacagttg
     1981 tcgtttccaa tgaaaatgta tgaccgagaa ttagtcgctc aaaaaattga aacaatgata
     2041 caagaaaacc attccattga ttctaggaaa ttaacggatc ggtatggttt ttcaacgaga
     2101 tggattcaaa gtattatcag acaacaacaa aaggagcaac atgtacatgg ctaatgaaga
     2161 aacagtaggt ttgagaatca tagaagaagc attcaaatta ccaatggtca aagttgatcg
     2221 tcgagcattt ttgattcaaa catttcaagg gaatgtttct gatattgaag gactcataga
     2281 aaagggtcca caagcatttt tatccaaaga aatgctagat cgtgaagcac aaaaatgtat
     2341 tcaatcgatt accttgaaat caagtgcggt atcgttagca actggactgc caggaggtat
     2401 cgctatggca gcaactattc cagcggatct ccttcagttc tttggttatt cattacgatt
     2461 agcacaggaa atttcgtata tttatggatt taaggattta atgggagaga ctggagagtt
     2521 cagtgaagaa gcaaagaata cgctgatatt gtatttggga attatgcttg gagtaacttc
     2581 tgcaggttct gccgtacgag cgatgtctgg aaagctgtct acacaagcat tgaaaaagat
     2641 tcctcaaaaa gcattgacac aaacgctgta ttatccagta atcaaacgag tactaggtat
     2701 ttttggtaca aagttaacta aaaatacctt tgcgaaaggt atttctaaag cagtaccagt
     2761 tgtgggaggc gttgtttcag gaggcatcaa ttacttgtct atgaaaccaa tggcaacgaa
     2821 gcttcaaaca gagttaggga aaaatgtagc gtatacagaa aaaaatctcc aacaagattt
     2881 agatatttta gaaggtgaat ttgaagaagt atcttctgat atcgtgtctt ctgatacagt
     2941 tctttctcaa ttagaaagac tttctcactt gttggaaacg aatatgatta cagaagaaga
     3001 gtttcaacaa ttgaagcaag agctgttaaa gaaataatta tttagaaaaa gcaaccactt
     3061 atacgggtgg ttctttttct tgaatgaatt caaatttatt attaggagaa gaaaaaggat
     3121 gaaagcaaaa cgattattcc tattaggcac gattctttta cttggaggat gttctactgt
     3181 ctctcaaaat gaacaaaaga aacaagtgga ttcaacaact tatgaaactg aaacaacaag
     3241 aaaggaatct tctacaaaag aaatagaaac aactactcaa gaaactaaaa caacagagtc
     3301 ttctagtaag gcaacgagtt cagatacgag tgattttcaa ataaaacaag aattatggga
     3361 tacaaataaa gcatctcaat tagcaacttt tgtcccacaa tggggaaaga cattaggtca
     3421 agaatataaa agctacaatc ctcagaacaa tgtaagtctg tatggtacgc ctttaccaag
     3481 tgcagttatt gatggaaatt ggaaaatggc aatcaatgaa gcaccagtta ctgtacaatg
     3541 gtcagaagat ggaactggaa atgcaggatt taatttagta gcagtttatt ctgatgtaga
     3601 aactggagaa tatttagggc agcatgttta ctttttcggt tttcaaaatg gacaacctaa
     3661 agtttattta acgcaacaaa atcaaggtaa tgaaaataat tatctgtact ttaacgaaac
     3721 tcaaaatcaa cagctaaaac aaggatttac agatatcatt aatggacaaa ctccccaaac
     3781 accagtagta gaagaatcta ctcaacaagc aacaactaca acgacacaat ctacaagtag
     3841 ttcaggaatt tcaaattggg aagctgctaa agaaactgta ctaaataata aagcgttatg
     3901 gatttatgag tctccagata aaaatgaagc aaatactaat attattgaat ccacccaata
     3961 tagaaaagat ttaatgcaag atgaaaatgg agaattttat ccagttgata tttctactga
     4021 tgatattgga aatacggtaa tgaatggagg aaaacaatt
//
LOCUS       DAZUMT010000163         4011 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000163 DAZUMT010000000
VERSION     DAZUMT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.170"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG8FM_002788"
     CDS             <1..135
                     /locus_tag="ACG8FM_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5095376.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG8FM_002789"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG8FM_002789"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS5095377.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="ACG8FM_002790"
     CDS             complement(2412..2663)
                     /locus_tag="ACG8FM_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS5095378.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="ACG8FM_002791"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="ACG8FM_002791"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS5095379.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="ACG8FM_002792"
     CDS             3613..3831
                     /locus_tag="ACG8FM_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095380.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1120 a          850 c          735 g         1306 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat t
//
LOCUS       DAZUMT010000164         3970 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000164 DAZUMT010000000
VERSION     DAZUMT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.171"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACG8FM_002793"
     CDS             75..503
                     /locus_tag="ACG8FM_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS5095381.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACG8FM_002794"
     CDS             490..732
                     /locus_tag="ACG8FM_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095382.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACG8FM_002795"
     CDS             complement(1032..1247)
                     /locus_tag="ACG8FM_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095383.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACG8FM_002796"
     CDS             1354..1668
                     /locus_tag="ACG8FM_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095384.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACG8FM_002797"
     CDS             1772..2950
                     /locus_tag="ACG8FM_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5095385.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(3131..3316)
                     /locus_tag="ACG8FM_002798"
     CDS             complement(3131..3316)
                     /locus_tag="ACG8FM_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095386.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            3353..3646
                     /locus_tag="ACG8FM_002799"
     CDS             3353..3646
                     /locus_tag="ACG8FM_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS5095387.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3734..3880)
                     /locus_tag="ACG8FM_002800"
                     /pseudo
     CDS             complement(3734..3880)
                     /locus_tag="ACG8FM_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1357 a          591 c          778 g         1244 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga
//
LOCUS       DAZUMT010000165         3955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000165 DAZUMT010000000
VERSION     DAZUMT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.172"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACG8FM_002801"
     CDS             complement(<1..448)
                     /locus_tag="ACG8FM_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095388.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFA"
     gene            715..2395
                     /locus_tag="ACG8FM_002802"
                     /pseudo
     CDS             715..2395
                     /locus_tag="ACG8FM_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            2365..2583
                     /locus_tag="ACG8FM_002803"
     CDS             2365..2583
                     /locus_tag="ACG8FM_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS5095389.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2603..3493
                     /locus_tag="ACG8FM_002804"
     CDS             2603..3493
                     /locus_tag="ACG8FM_002804"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS5095390.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3511..3795
                     /locus_tag="ACG8FM_002805"
     CDS             3511..3795
                     /locus_tag="ACG8FM_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095391.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1389 a          656 c          799 g         1111 t
ORIGIN      
        1 tagcaaaaat cctataaaat tcatctaaat agcgataata aatccgattc agctgatcat
       61 taagatcgaa gtatacaaaa ttattctgta gtttataaaa aaacggcgaa tgaagatgtg
      121 ttttcatatg accaaaatat aataattctg aaatttctaa aggtgtcaat tctttcagca
      181 tcgttaaatc agtaaagtcg atccacttca attcatcgaa tccacgcctg ttacgagaag
      241 aggaaaagaa acgctgaata ttttctgtac cacggattac tttcagccct gtatgggatt
      301 catattctcc agcagaagca gatggatcaa gcaagagcaa gttttgagga aaatgcacga
      361 tactgtttgc aaagtcagta gcagtcagcc cttttgttaa aacagcatta cttgtggtat
      421 caatatgtac ataaactgac tcagtcatca tgttcctcct cttatttcct cttaatctct
      481 tttattttac aacatttgca ttaaaaaagc ttgtttcttt tattttaaaa ttccattaca
      541 aaatcacgtt ttcattgaaa acactctcat tttcaattaa tttttgtgat ttttgtattt
      601 ttttatttaa aggaggtttt atgaattgtt ttcttatgga atctacaaag tcgaatacga
      661 gactcatgac ctttttatga cgtactggta tacttgaaga aaaaggagag cgcgatggag
      721 ataaaaaaag tttacttttc acagaaaatg tcgctgtatt cgcctatcca gcaagcaaaa
      781 caaccccttc cattttttca aagtcatcaa gaaagtagag acgctttttg gcgttctgca
      841 aaaaagcaat gtgcttttaa agcagtatcg aaaaaatata ccgtctattt tactattgct
      901 aaaagtagag gagatgttat ttttaataat ctgttgattg aaggtgaaac ttttgaatgg
      961 aaaaaatttt ttcggtatat ggaagcttgc gctcgttttt ttattaaaga aaactgttgt
     1021 tttttatttc aatctcctct atcagaagaa tggttgcgta tttttcataa aaacggctat
     1081 caaggaacca cacaattaaa taaaaagctg gtctatcata cagcacttgt tttaggtgga
     1141 ggaggagcgc acggtgctta tcagattggt gtgtggcagg cgctgaagga acatgatatc
     1201 aactttgaga ttatcacagg tacctcggta ggtgcgttga acggcgcatt gattttacaa
     1261 ggagatatga aaaaagctgt cgacaagaca agtgcttgcg ttaccagaaa tggctgcagt
     1321 tgaagacctt cgtgaacgct tctatcgaga aagacgccaa atgacgaaaa cagcactaat
     1381 tgaaggcggg gtttcttctg ctccattgga aaagatggtc aaagacaatt tggatttgtc
     1441 cttactcaaa cataatccca aaatacgttt atatacggtg tctactaaac tgccagaact
     1501 agcggaagta gtgactgata tccaacagac aaaaacagaa gaaataccag attggatact
     1561 cgcctctgct tcattttatc ccgctatggc ttatcgaaag atcggaaaaa ataaatatgc
     1621 agacggtggg tatcgcaaca aaatacctat tgatattgcg attaacaaag gagcaacaga
     1681 ggcatttgtc gtagatgtgc aagggccagg cccagcgaaa aaaattcgta tgccagacat
     1741 ttttatccat tggaaatgtc aaacattgtg gacactaggc agctttctgc tttttgatag
     1801 ccaaaggaac caattaaatt tacaattagg ctatctggaa atgaaaaaac gattaggcta
     1861 ttactatggg aactggtata catttcattc tgttaaacct gcaagaacat actggcgagg
     1921 atttttgtct tacttaacaa aagaaattca gttagaatta tcttttttca aaagcataaa
     1981 attttggcaa aaactgcgta atttatataa gaacagggtc gtaccagaga catgcggatt
     2041 ggcgatgcta gaacttttag caaaaaaaca ttttttactg cccaatgaaa tctatcaagt
     2101 agacatgatg attcaaatga tctgccaaca agatataaaa agtatgccgg atgatttatt
     2161 ggcacaaatt ggtcaattaa gtactgaaga atggcgaagg tatcgaaaat accagcaaaa
     2221 aatacaaaat gagcgcacga aaactgttta ttttgattat ctgctacaag gaaaacgcaa
     2281 ggatcgtttg cagcatgaat tgcttaagca accagttgac acattactga tcctatattt
     2341 atattatctc aaggaggaac aaccatggca caagaatttt catatgaaat cgtagaagaa
     2401 atcgctatat tgtcagaaaa caacaagggg tggcgtaaag aactgaacct agtcagctgg
     2461 aatggtcgcc cgccaaaatt cgatcttcga gattgggcgc cagaccatga aaaaatgggg
     2521 aaaggcctga cattgacgaa cgaagagttt gaacaactgc aaaaagcaat agaaaatatg
     2581 taatgaggaa ggaccctgtc gaatgaaaag tatgactggt tttggcaaag ccatccgcga
     2641 aacaaaagcg tatcagttag atattgaaat aaaaagtgtc aaccagcgtt ttctagatgt
     2701 tcaaatacga agtcctaaaa tcctgaactt tgttgaaaac gattttcgtc agcttattaa
     2761 acagcagtta agccgcggtc gaatcgaagt atttgtgaat ctagcttata ttggcaaaaa
     2821 cgaaaaacag atcgtcatca actgggaatt gattgattca cttatcactc aattgacaga
     2881 cggaattcaa caacgatacg gtagtcaaaa tcaagtgtca attagtcgga tggcggaacg
     2941 cttcgcgctg gacgaaaact tcgtaacggt agaagaaaaa ccggaagaga gcatggaaga
     3001 attggcacag ttagctctag atacattgaa aagcgcactt gattctattg aggcgagtcg
     3061 agaaaaagaa ggtcaagctt tagctttagt gttgaaaaag aatactgaag aattcaaaga
     3121 agtactaaaa aaactcgata gttttgtaga aatctatgaa aaggaatttc aaataaggta
     3181 tcaaaaaaag ctggaggaat ttcttggagc gaaagtcgat gagcaacggt tgttgacgga
     3241 gctagccatc ttgttggaac gcggagatat tcacgaggaa ctggatcgtc tggcgatcca
     3301 tgtacaaaaa ctagatgaac ttatcgaagc acaatgtcct gtaggtcggg aattagattt
     3361 cctgatccaa gaaatgaatc gggaaatcaa cacgatcggt tccaaatcaa atgcaatcga
     3421 aattaaaaat caagtcgtcc agtctaagac gatcttagaa aaaatcagag aacaagtaca
     3481 aaatattgaa tgacgaaaga ggtagagata atgaaatatt atgacgtaac gttccacgaa
     3541 ttaagtggaa aaactgtggt taaacgggat atcccttctg aaaaaaacgg ctttgacgta
     3601 tggaaagatg cctgtgcaga ctataatgag aacgaactgt ttattttgat caacgacggt
     3661 gcttacgtga cgatgaatcg gaaatttatt gttcgaattg acacagaaga ggtagaagat
     3721 ccgacagaaa aagcacgttc tcgaaaagat gagatcatgg gtgtagtgaa tactttgtcc
     3781 aatatgggct tttagcaagg atgctgaaca aagaaataca ataaataatc agaattggat
     3841 ttcttcgctt agcttctttg ctctaggaat acatttaaag gttgtgacaa aattcgtgtc
     3901 acaatctttt tttattttga ataagtgatg ttcaaaaacc attttctcgg tatta
//
LOCUS       DAZUMT010000166         3931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000166 DAZUMT010000000
VERSION     DAZUMT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.173"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8FM_002806"
     CDS             <1..156
                     /locus_tag="ACG8FM_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS5095392.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACG8FM_002807"
     CDS             175..1452
                     /locus_tag="ACG8FM_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5095393.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="ACG8FM_002808"
     CDS             1462..2118
                     /locus_tag="ACG8FM_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095394.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="ACG8FM_002809"
     CDS             2118..3494
                     /locus_tag="ACG8FM_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5095395.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3931
                     /locus_tag="ACG8FM_002810"
     CDS             3624..>3931
                     /locus_tag="ACG8FM_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095396.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     "
BASE COUNT         1585 a          418 c          688 g         1240 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta a
//
LOCUS       DAZUMT010000167         3795 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000167 DAZUMT010000000
VERSION     DAZUMT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.174"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1989
                     /locus_tag="ACG8FM_002811"
     CDS             <1..1989
                     /locus_tag="ACG8FM_002811"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HFS5095397.1"
                     /translation="LKQGKQDYQIIGYLEDDLNKIKINSDDVMYFFMTPLSDFPSSVI
                     DNVEGIQIYSNTLTKFGFIDDINESHSTRAQSLKEFLDPYIIVCTPKIKRNHEQARNY
                     YQGDDLELIPKPKSEVFKNLIKENESLVPIPVFSINNSDSVIKDETSFENQLLNGKLL
                     GKIINFSKDNEDYPLNVFWENTDGSRVLFGEIIGQNNSMYGVAYRIEKDNFYKKFIDE
                     GSKNYVGYDDFINQTKDIIFVPYLLLQKIIETKKDINEGIENISDSKISPVSSIITEQ
                     TQIDISELEDKHAIQQNDHFSEHNEFIERFNQLTIRSKLHYSKRDLWNFHTAMLGDSL
                     VVLAGLSGTGKSQLVTSYAKALQLSDTQIKFISVRPFWEDDSDLLGYADTVNSVYRPG
                     DSGLIDALLEASRHKENIYMVCFDEMNLARVEHYFSQFLSVLEMEPNTRKIKLYNEDL
                     ENKLYNSSTYPSTIEVGQNILFVGTINTDESTHQFSDKVLDRSNIISLELVPFHEISE
                     VNDYIQSDQKKRNQLKYDDFKNFKNSNPTNGLTQKEKEMFWKLHLAINSKDKNIGIGW
                     RILKQIDEYLKNLPVQNDLSRKEAIDIQLVQRVLSKIRGSDEQLSELVGKWNENDLKQ
                     VGEFEQIINEYPESSDFKYTKKILLQKARELNLHGFTL"
     gene            1973..3763
                     /locus_tag="ACG8FM_002812"
     CDS             1973..3763
                     /locus_tag="ACG8FM_002812"
                     /inference="COORDINATES: protein motif:HMM:NF016309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HFS5095398.1"
                     /translation="MDLPFSLKLNFGYKSEEQEYELLFFQDEASLLEYTPVKVKEFSN
                     LSFTFNSIDKHAKLYIPELDDYLIENQVKEENGDICYIPSDDNIIIYNRDQGKTVPLI
                     PGYYTLYVKIGETIFYSRFEIILKDLGTPEWEKMKIEIEKMVGGLARTFIPKKTSRRE
                     ITTSNYLMNDQLFQRILEFENNIPQAIVSLESLKKEAKYRIDKRYNWKPIGSKNLVDQ
                     KTISQLQIHPEKRGLLYTPSRIIKYDIIENQWIKFILSYFKNFSLLADRYLEKILQGI
                     IEEKKREDKFTSAYSQSVQNYNTNSYNRKIENLKNNQQKIKHFYAYILEFLDTPVMKN
                     ISSRRPINVPKSLVMNPKYNTIYKMYLKSRNNRNTLNFSNRYQYYWKSTDVLYEIWCF
                     IKIIRSLSLQGYTPISGWIFENDILSQELPFLHEDTTVVLSKNDIRINLVYNNSMEKE
                     YFSNTLDIPVKTSSKRNKPDIRLDIILQDKYYIGSLIFEVKYKKLDNILDNDKGRQRQ
                     QLMAYKQNTMSSILAFPEILTRSLQAVSAVFALYPSNEGRKKTAPKYYEKEGIFFHLL
                     NPSSDEKELSVKIQTSIEERISLFNQQSRN"
BASE COUNT         1521 a          519 c          563 g         1192 t
ORIGIN      
        1 ttaaaacaag gtaaacaaga ttaccaaatt attggttatt tggaggatga tttaaataaa
       61 attaaaataa actcggatga tgtaatgtac ttttttatga ctcctctttc agatttcccc
      121 agtagtgtga ttgataatgt agagggtata caaatatata gtaatacgct tactaaattt
      181 gggtttattg atgatataaa cgaatctcat agtactcgtg cccaatcact taaagaattt
      241 ttagatcctt atattatcgt ttgtacacca aaaattaaaa gaaatcatga acaagcaaga
      301 aattactatc aaggtgatga tttagaatta atccccaaac caaagtctga agtttttaaa
      361 aatttaataa aagagaatga atcattagta cctataccgg tcttttctat aaataacagc
      421 gattctgtaa tcaaagacga gacaagtttt gaaaaccaat tactaaacgg caaactactc
      481 ggaaaaatta taaatttctc aaaagacaat gaagattacc ctttaaatgt tttttgggag
      541 aacactgatg gatcaagagt tttatttggt gaaataatcg gacaaaacaa ttcaatgtac
      601 ggtgtagcat ataggataga aaaagataat ttttacaaaa agtttattga tgaaggtagt
      661 aaaaactatg taggttacga tgattttata aatcaaacaa aagatataat ttttgtgccc
      721 tatctattac ttcaaaaaat tatagaaact aaaaaagata ttaacgaagg catagaaaat
      781 atctctgatt ccaaaatatc tccagtaagt tcaattatta ctgaacaaac gcaaatagat
      841 atatctgagt tagaagataa acacgctatt caacaaaatg atcacttctc tgaacataat
      901 gaatttatag aaagatttaa tcagctcaca attcgtagta aattacatta ctcaaaacga
      961 gacttatgga attttcatac tgctatgttg ggtgatagtt tggtggtatt agctggacta
     1021 agtggtaccg gtaaatcaca attagtaaca tcctatgcaa aagcacttca attatcagat
     1081 acacaaataa agtttatttc tgtgagaccc ttttgggagg atgattctga tttattgggc
     1141 tatgctgata ctgtaaattc tgtctatcgt ccaggagata gcggtctaat tgatgcacta
     1201 ttagaagcat caagacataa agaaaatatt tatatggttt gttttgatga gatgaattta
     1261 gctcgggttg aacattattt ctcccaattt ctttcagttt tagaaatgga gccaaatact
     1321 aggaaaatca aactgtataa cgaagatcta gaaaataaat tatataactc ttcaacctat
     1381 ccttctacaa ttgaagtagg acaaaacata ttatttgtag gaacaataaa tacagatgaa
     1441 tctactcatc aattctcaga taaagttttg gatagatcaa acattatttc acttgagtta
     1501 gtaccattcc atgaaattag tgaagtaaat gactatattc aatcagatca aaagaaacgt
     1561 aatcaactta aatatgatga tttcaaaaat tttaaaaact ctaaccctac taatggtttg
     1621 acccagaaag aaaaagaaat gttttggaaa ctccatttag caattaattc taaagacaaa
     1681 aatatcggta tcggttggag aatattaaaa caaattgatg aatatttaaa aaatcttcca
     1741 gtgcaaaatg acttatcaag aaaagaagct attgatattc agcttgtaca gagagtatta
     1801 agtaaaatca gaggttctga tgaacaatta tcagaattag ttggtaaatg gaatgagaat
     1861 gatttaaaac aagtgggtga atttgaacaa ataattaatg aatatcctga gtcttcagat
     1921 tttaaatata ctaaaaaaat tcttttgcaa aaagctaggg agttgaattt acatggattt
     1981 accctttagt ctgaaattaa actttggtta taaaagtgaa gaacaagaat atgaactttt
     2041 attttttcaa gatgaggcat cgttattaga gtatacaccg gttaaggtaa aagaattttc
     2101 taatctaagc tttacattta acagtataga caaacacgca aaactatata taccagaatt
     2161 agatgattat ttgattgaaa atcaagtaaa agaggaaaat ggagatattt gctatattcc
     2221 aagtgatgat aatataatca tttataatag ggatcaaggt aaaacagtac ctcttattcc
     2281 aggttattac actctttacg ttaaaatagg tgaaactatc ttttattctc gatttgaaat
     2341 aatacttaaa gatttaggta caccagaatg ggaaaaaatg aaaatcgaaa ttgaaaaaat
     2401 ggttggcggt cttgctagaa cttttattcc taaaaaaact tctcgtcgtg aaattactac
     2461 ttcaaattat ctaatgaacg atcaactttt tcaacgaata ttagagtttg agaacaatat
     2521 acctcaagca atagtttctc ttgaaagttt aaaaaaagaa gcaaaatata gaattgacaa
     2581 acgatataac tggaaaccaa ttggaagcaa aaatttagtg gatcaaaaaa ctatttcaca
     2641 gttgcaaatc catccagaaa aacgtgggct actatataca cctagccgca taataaaata
     2701 tgatattatt gaaaatcaat ggatcaaatt tatcttatct tattttaaaa atttttcact
     2761 tctagctgat agatatttag agaaaatcct acaaggtatt attgaagaga aaaaaagaga
     2821 agataagttt actagcgcgt attctcaaag tgtacaaaac tacaatacaa atagctataa
     2881 tagaaagatt gaaaatttga aaaacaatca gcaaaagata aaacattttt atgcttatat
     2941 ccttgaattt ttagacacac cagtaatgaa aaacatttca tcaagaagac ctataaatgt
     3001 tccaaaatca cttgttatga accctaaata taacactata tataaaatgt atttaaaaag
     3061 tagaaacaat cgtaatactt taaatttttc aaatagatat cagtattatt ggaaaagtac
     3121 agatgtactc tatgaaattt ggtgctttat aaaaattatt agatctctat ccctgcaagg
     3181 ctacactcca atttctggat ggatttttga gaatgatatt ctttcacaag aattgccgtt
     3241 tttacatgaa gacactaccg tagtattgag taaaaatgat atacgtatta atcttgtata
     3301 taataattcc atggaaaaag aatatttctc caatacacta gatatcccag taaaaacctc
     3361 ttcaaaacga aataaacctg atattagatt agacattatc cttcaagata aatactatat
     3421 cggaagctta atatttgaag taaaatataa aaaactagac aatatacttg ataatgataa
     3481 aggtaggcaa cgtcaacaat taatggctta taaacaaaat actatgtcaa gtattctagc
     3541 atttcctgaa attcttacac gttctttgca agctgtctct gctgtctttg ctttatatcc
     3601 ttccaatgaa ggaagaaaaa aaactgcacc taaatattat gaaaaagaag gtattttttt
     3661 tcacttatta aatccttcat cagatgaaaa agagctttca gtaaaaattc aaacaagtat
     3721 tgaagaaaga atttcattat ttaatcaaca aagtagaaat taaaaagatg aagagcgtct
     3781 ctttatacaa gagag
//
LOCUS       DAZUMT010000168         3720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000168 DAZUMT010000000
VERSION     DAZUMT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.175"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..355)
                     /locus_tag="ACG8FM_002813"
     CDS             complement(<1..355)
                     /locus_tag="ACG8FM_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095399.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(615..1163)
                     /locus_tag="ACG8FM_002814"
     CDS             complement(615..1163)
                     /locus_tag="ACG8FM_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095400.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1164..2021)
                     /locus_tag="ACG8FM_002815"
     CDS             complement(1164..2021)
                     /locus_tag="ACG8FM_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5095401.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2307..2593)
                     /locus_tag="ACG8FM_002816"
                     /pseudo
     CDS             complement(2307..2593)
                     /locus_tag="ACG8FM_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2583..2912)
                     /locus_tag="ACG8FM_002817"
     CDS             complement(2583..2912)
                     /locus_tag="ACG8FM_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5095402.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2989..3261)
                     /locus_tag="ACG8FM_002818"
     CDS             complement(2989..3261)
                     /locus_tag="ACG8FM_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5095403.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3261..3524)
                     /locus_tag="ACG8FM_002819"
     CDS             complement(3261..3524)
                     /locus_tag="ACG8FM_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5095404.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1092 a          754 c          473 g         1401 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat actctatttg agagtcatct aattggctca
      241 taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc atgaacccaa
      301 acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt ttcatctaca
      361 tctcctccta atagttttat tttagagtta gctctattac ttatttacta actcaacaac
      421 agattatcat acacttgtta atgaaatcac aatatctcat taaaacacac cctaaaatca
      481 taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa aataaatccc
      541 ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt agttacttag
      601 ttcttctttg attattatct aactttatta ttcattcccc accctttgcg cagtgagtct
      661 tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc atctaattct
      721 ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc attaaacaaa
      781 tcttgcttat cttcttgcgt aagattctgt ttaatatact catctaaagc ttttctaagc
      841 aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga agtcgcattt
      901 aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa ctttctgatt
      961 ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga ggaagttttt
     1021 atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat tgcttgctgc
     1081 tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt cccaaaaata
     1141 tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc gtgttcttcc
     1201 aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg ttttaagaga
     1261 cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa gcgcgcattt
     1321 ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg tttatacatt
     1381 tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc aacatataaa
     1441 tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat cgcatttctc
     1501 atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta catcgcggat
     1561 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
     1621 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
     1681 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
     1741 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
     1801 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
     1861 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
     1921 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
     1981 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
     2041 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
     2101 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
     2161 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
     2221 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
     2281 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
     2341 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
     2401 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
     2461 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
     2521 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac
     3061 aacccggtgt tgtctattca atcttctaga atacctacct tcatgtgtag gttttaactt
     3121 ctcaaatgat tgggtaggga gatagggatc ttcctttaaa gtctgaataa ccttctcaaa
     3181 ttgaggtttc aaattagact gtttgatttt ctttaaatca gctttagccg agtttttaat
     3241 catcacagaa tagttattca ttataagtta tcccaatcaa tttcatcaac attagtaaat
     3301 ccactatcat ctttttctcg cttacgaaca acacccaaag tgcctatttg ctcaagtaat
     3361 agcgtttctt tgattgcttt ccaatcatcc aaaccaataa gcaccgcatt attttcactg
     3421 cgctcactaa tgatttcaat ttccgtatga ttctcattta catctttcaa caacttataa
     3481 aaatttttcc gtgctgagct aggatttaag actgtttttg ccatatctac tccccctcta
     3541 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
     3601 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
     3661 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
//
LOCUS       DAZUMT010000169         3691 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000169 DAZUMT010000000
VERSION     DAZUMT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.176"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1183
                     /locus_tag="ACG8FM_002820"
     CDS             17..1183
                     /locus_tag="ACG8FM_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS5095405.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            1229..2725
                     /locus_tag="ACG8FM_002821"
     CDS             1229..2725
                     /locus_tag="ACG8FM_002821"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS5095406.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            2806..3489
                     /locus_tag="ACG8FM_002822"
     CDS             2806..3489
                     /locus_tag="ACG8FM_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5095407.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            3498..>3691
                     /locus_tag="ACG8FM_002823"
     CDS             3498..>3691
                     /locus_tag="ACG8FM_002823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095408.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         1262 a          641 c          792 g          996 t
ORIGIN      
        1 aataaggaga ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag
       61 cgggctagaa gcactggttg gtaaagaatt aagagatctt ggaatcgaat gccaagttga
      121 gaacggacgt gcacgttttg aaggaacaat ggaaacgatc gctacagcta atttatggtt
      181 acgtacagca gatcgagtaa aaatcgtggt tggggaattc gatgcgtata cgtttgatga
      241 attgtttgaa aacgtaaaag cattgccttg ggaagatttt cttccactag atgcagcttt
      301 tcctgtagct ggaaaatcga tcaagtcgaa actatatagc acacctgatt gccaagcgat
      361 cacaaaaaaa gcaatcgttg aacgtctccg tacctattat caccgtccag cttctgtacc
      421 actgacggaa actggcgcac attttcagtt ggaagtagct ttgctgaaag accatgtgat
      481 ggtaactctg gatacgacag gaccaagttt attcaaacgt ggttatcgct tagaaaaagg
      541 tggagcacca ttgaaagaaa acatggcagc agcacttatt atgttgacaa attggcgaaa
      601 agaccgtcca ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt
      661 aataggccat aatatcgcac caggattcaa tcgtgagttt gcttgcgaat cttgggattg
      721 gttcagtcaa gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag cagattatga
      781 tattgaatta gatattactg gttcagatat caatggacgg atgattgaaa ttgccaaagc
      841 caatgcagaa gaaatcggat taggctcttc gattaccttt aagcaacaag cagtcaaaga
      901 cttcaaaacg gataaagaat acggggtcat cgtggctaat ccgccttacg gagaacgtct
      961 aggagaagaa gaaactgttc ggaaattata taaagaaatg ggcgatgtat tccgtccatt
     1021 aaaaacatgg agtaaatata tcttgactag cgacttagcg tttgaggaat tttacggaca
     1081 aaaagcaacc aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc tattccagta
     1141 ttggggaact cgtccgccta gaaaaaaaga gaaagacgaa taatcttact aattgattca
     1201 ttcaattgga tgtttggagg aatagggaat gaaagaaaaa gagtttttac aagaagtaaa
     1261 agaaatcaat cttctacaac aagctttagg aatcctcgat tgggatacac aaacaggtat
     1321 gcctgaaaaa gctagtgcat accgaagtga agtagacagc tatctttaca gcatttattt
     1381 ttcaaaaaaa atcggtccaa aaatcaaaga agccattcgc tatttttctg aacatccgga
     1441 agaattgtcg gaagttggaa ctgccgtttt tgccaaagta aaagaagagt atgatttgga
     1501 acatcgcgta ccggaagatc ttatgagaaa atatacagct gccgtttctt cagcacacgg
     1561 acaatggcaa aaagctagag aaacacaaca attttctgat tttcaacacg cattgaatca
     1621 aaatattgaa ttaacaaaac agttgatacc ttactggaga aaagatgaaa agacgaatta
     1681 tgatgttttg ctgaatcaat acgaacccgg gatgactgtc gaaatccttg ataaggtatt
     1741 tgatcagctt aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa aagggactga
     1801 acctgaaaca gactttttaa atcggagaat gacgaaaaaa caacagaaga gatttgtagt
     1861 gaaagtaatc gaacaacttg gttatgattt tagtcgtggt cgtttagatg acactgtcca
     1921 cccatttatg actggaatta atccgaatga tgcacgaatc acgacacgat ggaacgaaac
     1981 agatttcaaa atggctgttt ttggagtgat ccacgaggct ggtcatggta tgtatgaaca
     2041 agacatcgat gataaatatg cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca
     2101 tgaatcacaa tcgttgttta acgagatcat tattggaagt agtcgcagct tctggaaaaa
     2161 gcaatatcct ttctttcaag aatgtgcaga aggaacattc gatgatattc cttttgacaa
     2221 attttatcgt tcattgaacc atacaaaatc aagtttggtt cgtatcgagg cagacagttt
     2281 gacttaccct ctccatatca tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg
     2341 ggtagatgtt gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat
     2401 acgaccaaca aatgatgtgg aaggcatcct acaagacgtt cattggtcag gtgcaagctt
     2461 tggttatttt ccctcttatg cattagggta catgtatgcg gcacaattac ttcattcttt
     2521 gcagcaagaa atagatgtgg aaaatgttct agcgtcagac gattattctc caatcaaaaa
     2581 ctggatgtca gagcatatcc atcaatatgg tgcatctaga aaaccaaatc aattaatcat
     2641 ggatgcaaca catgaaccgc ttaatccgca atacctgatc gactatatga aagacctgta
     2701 ttttaatgta tatcaggtaa aataaaatac aatttacaaa gatagctgaa attcatctaa
     2761 tctatagatg ttttcagcta tttacttaag aaagaagtgg aacgaatgga taaattacaa
     2821 gtgatcgctg attatagtat ggaccaatta gcgcatgatc aaacaggaca tgggagcgat
     2881 cataccaaaa gagttgtcaa gttagctgaa aaaattttgg agacggaacc gcaggcagac
     2941 cgctttgtga cgcttgcagc agcttaccta catgatacga ttgatgataa agtcgttaaa
     3001 aatgagaacg aggcaaagca acagcttcgt gtattccttc gctctttgcc aatagtagag
     3061 gaacagatct cgatgatttt ttccattatc gaaaatatgt ccttttctaa aaatctctct
     3121 gaagcggccg aactatctct ggaaggtaag atcgttcaag acgctgatcg tatcgaagca
     3181 ttaggagcaa tcggcatttt gcgtactgct tatttcggcg gtggacatgg gcacccgatt
     3241 tttgatcctg aactgtatcc gcaaacattc aaggacaaag aaaattaccg aaaaggcacg
     3301 acagtgatca atcattttta tgaaaaactg ttcttgctgt ctgataaaat gaatactgat
     3361 tacggatatg aagaagccaa acgaagagaa accttcatgc gtgaattctt agaagaattt
     3421 tttctggaat ggtctattga cgatgaaaat tttactccag acaattggat caaaaataac
     3481 aaaaaatgat ggaggttgtg acagaagtgg tcagctccaa gatatttcgg atcattttcc
     3541 gtaaaagtta cttctggaac actgtttacc aagaggtcgt gagcctgccg ttcagctccg
     3601 agtcacaagg aacaatttca aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg
     3661 attcggaatt ggcaggagaa caccgtttat t
//
LOCUS       DAZUMT010000170         3449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000170 DAZUMT010000000
VERSION     DAZUMT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.177"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..553
                     /locus_tag="ACG8FM_002824"
     CDS             <1..553
                     /locus_tag="ACG8FM_002824"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5095409.1"
                     /translation="KTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIYGE
                     GGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEALAI
                     GVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQKGI
                     KLRKELTSKNISNIWYEYLNQFF"
     gene            572..1996
                     /gene="wzy"
                     /locus_tag="ACG8FM_002825"
     CDS             572..1996
                     /gene="wzy"
                     /locus_tag="ACG8FM_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFS5095410.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIG
                     IYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNG
                     IYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNG
                     GYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIV
                     KNFVYGVLIILLINFFLQQFNKRL"
     gene            2042..>3449
                     /locus_tag="ACG8FM_002826"
     CDS             2042..>3449
                     /locus_tag="ACG8FM_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS5095411.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAV"
BASE COUNT         1146 a          443 c          628 g         1232 t
ORIGIN      
        1 taaaactaca agtagtaaaa aatttattac tgcatgtaga ttggataaac aaaaaaattt
       61 gcccttaatg attgatgcaa taggaaaact tattcatgaa ggatatgagt gtgaactgga
      121 tatttatgga gaagggggat taaaaaccga gcttgaaagt tatatcaacc ataatggatt
      181 aacagaattt gttcatctaa aagaatttac aaataaaata catgaaaaga tgcaaaatgc
      241 ggcaggattt gtaatttctt ctgattatga aggtatatct aattctatgt tagaagcact
      301 ggctattgga gtgccagtta tcgctacaga ttgtccagta ggtggcgcaa gaatgtattt
      361 aggtgataga aatgaaatag gtcatcttgt tgaatgtaat aatctagata agttaacaga
      421 tgcaatgcgt agggtacttt tggatagaga aagtgtggta agaaaagcgc aaaaaggaat
      481 taaactaaga aaagaactga catctaaaaa tatttcaaat atatggtacg aatatttaaa
      541 ccagttcttt taaccaaaag gaggatattg tgtgaaagaa aaaataaata tattgatgtt
      601 ttcgttaacg ttagtcgcgt ttacaatatt ttgcgctttt tcattaaatc agaactattg
      661 gctaaattgg cttggaatat tgatttactt atatgcaatc tgtacatggc attggagaaa
      721 aggagattca atattttcat tatacacgat ttttttcact ctttttttat tatttagtta
      781 tgggcaatgc attatgtggg cattcggtat tggaacagat cgtggaatcg gtacaacagt
      841 agtagcttat ggtacgggga ttattcctag tgaatcagat atgataatgg taaaatggta
      901 ttcttgtatt tctatgtttg tatttcacat aggagcactc ttatttacga gaaaaaccac
      961 actaagaaaa gttagttggt atgaatctgg agatgctgat gccgatagaa agtttttatt
     1021 taaaataggg tgtatcattt ctataatagt agtaccgata gtttttgttt ctaaattact
     1081 agaggtacgt atagcattag tccatggtta taatgcatta tattatggtg attatgctac
     1141 acaaagtgga tatttacaaa ttattctata tctttttttt ccagccttaa tatgtttatt
     1201 aattggtagc aacttttcaa aagtaataac gagatttgtt tttattattt ttgctttata
     1261 ttctttatta ggaattatgt caggagacag aggaagttgg ttgtatagct ctattatact
     1321 catatgggca tacactcaat ataaaggaac ctttaattat aaaaaattaa taaagtggct
     1381 tattttagct gttataggaa tatatattct aaacgtaata acgaaagcac gtgatggtgg
     1441 aggactaagt aaacttactt taaaagattt tacatctgta tttgattcag atgattctcc
     1501 tttagttgat tctttttttg aaatgggggg gactatgagt attataactt ttttcctaat
     1561 aagtggtaat ggaatatatc cctttgctaa tacttatttg acctcactct taggctctat
     1621 atcaacaaga atgctatcaa tgtttggaat aaagtttgtt ttattagctg attggttttc
     1681 tcaggattat ttaggaatca cttgggggac tggattctca atgattgcag aagcctatgt
     1741 caatggtgga tacattgggg gtatagtcta tatttttatt attggtatag tcatcggtaa
     1801 aatcctttct aatagtatag acaaccaaga tattgaaaat catcctatga agatgttttt
     1861 ggctataact acagcaaata ttataatggg ttttcctaga gctgcttcat atttaattgt
     1921 gaaaaatttt gtgtatggtg ttttaattat attgttgata aactttttcc tacagcaatt
     1981 taataaacgt ctttaatatt taagcttaat aaaaagataa aacaagtcag gagttgcaaa
     2041 aatggaaaga actgctcggc tcaaaaaaaa cactatttgg gctctggtgt ttcaaataac
     2101 ttctattgtc tgtggtatga tcttgctacg tttgatatta acaaagtacg gatccgaggt
     2161 taatggaatg attagctcta ttaaccaatt tctacaaatt atttcttttt tacaattagg
     2221 agtaggcgct gtaatgcaat caactctata caaaccacta gcagaaaagg ataatttaat
     2281 gataagtcaa atcttagctt ctggagggaa gttttatcgg caaattgcat atattctact
     2341 agtatacgtg ggtttgttac ttgcagtcta ttatgttctc gttcctaatg aatttgggag
     2401 atcatataca atggcaatga ttgcaacatt aagtattaat tcatttgctc aatattattt
     2461 tggtattgtt aatactttgt tattaagtgc tgaccagaaa atttatatta cgtatattgc
     2521 acagacaata gcatttattt ctaatacagt tgcatgtgct ttattaatta gtctgaacac
     2581 agagatcctt atggtaaaac ttattactgc tataatttat cttgcttgtc caatatatta
     2641 tagatggtat gtggatcgaa actataatat aaagaggaat gtgtcatatg ataaggaacc
     2701 gatccctcaa aaatggaatg gggttgcaca acacgtagct tctgttgtac tagatagtac
     2761 tgatgttgta gtgttatcta tattttctaa cctgatatat gtatctatat ataatgtgta
     2821 caatttagta atatacggag taaaacagct attcatggct atgacaaatg gcgttcaggc
     2881 cttactaggg gaactttggg tcagaaaaga aatagaagtt ctttacaaaa catttgcatg
     2941 gttcgaatgg attgtacata ctttgacagt ttttattttt ggctgtaccg gtatactaat
     3001 cgttccattt attcaagtat atacaagagg agtaacagat gctaattata atcaattatc
     3061 attcgcagct ttacttgtaa ctgctaacgc acttcactgt ctgcgtacac cttatcatgt
     3121 aatgataaaa gcagctggta aatataaaga aactcaaaat agttttataa ttgcttcttt
     3181 gttaaattta ggtttatcat taattttggt taaaccacta gggctagttg gagtagcatt
     3241 aggatctcta atttcaatca catatcatac actttggagt gcaaattata attcaaaata
     3301 tttgcttaaa tggccgatga aaaaattcta taaacagtta tttgtaaaca tcttaactgt
     3361 agttctttct tttggggcga catcacaatt gaaaatgatg tcaatcacat atttggattg
     3421 gattgttctc gcaattaaat gtgcagtta
//
LOCUS       DAZUMT010000171         3448 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000171 DAZUMT010000000
VERSION     DAZUMT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.178"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1008
                     /locus_tag="ACG8FM_002827"
     CDS             208..1008
                     /locus_tag="ACG8FM_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS5095412.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            1024..1902
                     /locus_tag="ACG8FM_002828"
     CDS             1024..1902
                     /locus_tag="ACG8FM_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFS5095413.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1915..2895
                     /locus_tag="ACG8FM_002829"
     CDS             1915..2895
                     /locus_tag="ACG8FM_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS5095414.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            3166..3258
                     /locus_tag="ACG8FM_002830"
     CDS             3166..3258
                     /locus_tag="ACG8FM_002830"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095415.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
BASE COUNT         1193 a          552 c          707 g          996 t
ORIGIN      
        1 gttgtttttc tataaatcgt tgtaaagaat gactaagttg tattattttt tcacaaacat
       61 tcatattata atgtttcttt ttaataaaaa gattgttttt aagctaatta ttatatgtgt
      121 gttattgaac taactcttga cagataatca aaaagaattt atagttgtta ccgtagtagc
      181 tactaaaaat aaagtgagga ttaatcaatg gaaacgatta ttcaaatcat gaaaaaatat
      241 gatttttcag agatggagac aagagtgtat accacactat tagaaaaagg taatcttaca
      301 ggatatgaag taagtaaaat ctcaggtgtg ccacgctcga aagtatataa tattttggaa
      361 aaactattaa aaaagaattt gattgtcgtt aataaatcag aacctaaact ttatcatgcc
      421 atctcggcaa atgagttttt agagaaatta gaaaaaagtg ttaaaaatga tctttctttt
      481 cttaccaaga atctagggat gatcaaagaa aaagatgaag aagaaatgct gtggaaagtt
      541 gatggtatcg agtatgttct ggataaagta gagcatttgg tgaaaaacgc aaaagaaagt
      601 ttgctcattc aagtttggca tgagaatttc acggattcac tactaaaggc attgcagcaa
      661 gctgaaaaaa gagttgataa gtttgtactg attttattca gttctaccca tgaatatgat
      721 cttcccttag aaaagtatta tattcatggt tttgaaacag acaaacttgc tgactttgga
      781 gcaagatgga tcaacatcgt cgccgatgag caagaagtcg tgtttggtac cattaacgaa
      841 gaactgcaat caaccgatgt cacttggaca aaaaaccacg ccatggtcaa tctagcaaaa
      901 gaatatgtca aacatgatgc atatacacta aaagtgattg cagaatctcc tgaagaactt
      961 aaagcaaaat atggtgaggg ctttgaggga atcagaaaga tttactagta ggaggaattt
     1021 tttatgattc ggaatatttt gctgacacta attgtcgcaa tcaatctata ttttattatt
     1081 tactttctaa gagacctttt caaaaacaaa caaaatttca aagaagaacc aggggatatg
     1141 cgtctgttgc ccttcacgtc atttatcatc ttctttcttt caacatttgg cgtttccgac
     1201 tttgctatcg gaaccgtcct ttatccaaag ttgaaatggg tcagcatgaa aaaactaccc
     1261 ggaacgttga atacccaatg tgtagtaccg gtagccgtca tggcattgtc ttacatcact
     1321 gccatcaacg tagggatcaa gacactagct gtttgtatta tctgccaaat tatcggtgca
     1381 tatttaggac caagatttgt cgtgaagtta ccagagaaaa cgatcaaact gtttgtcggc
     1441 ataggattgg tcattgctgc attgttgatt tttgcaggac aaatgaactg gattccatca
     1501 aacggaacag ccagtgaatt gtatggcgga aaattaatct tggcaggatt tttactcttt
     1561 gtctatggtg cattgaacaa tatcggcatt ggttcttatg cgttaacgat ggtaactgtc
     1621 tacttgctag gcttgaatcc agttgcagcc tttcctatta tgatgggggc ttgtaccttc
     1681 tcagtaccga tcggcagtgt tcaattcatt aaatttaatg aatacagcag aaaaatcaca
     1741 ctttttactt caaccttcgg tgtattgggg gtattggtcg ctgtattcct agtcaaatct
     1801 ttagatacct atatcctgaa atggatcgtg attttggtct tactatacag tgcatatacg
     1861 atgttgggca gtcaattaaa aaaagttaca gcagctaatt aaggagcgat tcaaatgtta
     1921 tttcttaaaa aagaagatat tatcaaaagt ttttcaatga gagaagccat cgatgcggac
     1981 aaaaaagcac tgtcattgta ttcagcagga aaggcaagtg tgcctcttcg caccaatatc
     2041 gatgtgccaa aaagcaacgg acaaagctta tatatgcctg cttatgtaga aggtggcgaa
     2101 ggcgcgctag gtgtcaaaat cgtttccgtg tatcctgaaa acatcaagaa aaacttacca
     2161 agtgtaccag caacgatgat cgtattggat ccagaaacgg gtatggtatc cgcttgtttg
     2221 gatggtactt atttgacaca attaagaaca ggcgctgttc aaggagcagc aacagaactt
     2281 cttgcaagag aagatgctaa aattggcgcg ctgattggaa ccggcggaca agcacagtca
     2341 caattagaag caatgttgac cgttcgtaaa ttagaagaag ttcggatttt tgatattgat
     2401 tttgagcgtg ccagccaatt tgctgaaaaa atgatgcaac aattttctgt aacgatgcgc
     2461 ccaacaaaaa ccagtcaaga atgcgttgaa ggagcagaca tcattacaag tgtgaccact
     2521 tcaaaacgtg cgacattctc tgctgagtgg gtcaaaaaag gtgcgcacat caatggagtc
     2581 ggtgcttaca caccggaaat gtgtgaaatt ccacgagaaa taatcaaagc cgcagatgtt
     2641 gttatttttg ataccatgga cggtgttcta aaagaagctg gggacttcat ttcaccgctg
     2701 caagatgggt atattcaaag agacagctat catggggaac taggccaact gatcaatgaa
     2761 gaattgatcg gtcggacaag tggagaacaa atcacgatct tcaaaacagt tggttcggct
     2821 gtattagatg tggtagttgc gacagaaatc gttaagaaag ctaaagaaaa taatttggga
     2881 aaattgattt tctaattaaa aaggctgaaa ttaaaacctt tattattgcg tttaaaaaaa
     2941 gaaaacaaag aagtttgtaa tcaaatggaa actgacccca taatttaaat aaaataaaaa
     3001 acatctcttg ttgaattcta gtaatgaagc gaccctaaaa cttagaccaa aaaatctaac
     3061 ttttgggtgg agttgtcaag aaaaaccggg cacttagtta aaaaataagt tggacgggtt
     3121 atactaaaat tgacaccaaa ttaagagaaa gcgaagatct ggattatgcc aagacgttca
     3181 tttgatgaaa tatttaaaat taccgctgta aaactggtaa cggaagaagg gttttctgtt
     3241 aaagaagtta gtctatagct agaagctcgc tctaatagcg tgtattgatg agttcaagaa
     3301 gttgaaaaat atggataacg tacttttctc ggtagaggaa gtcatgaaat ccattcgcag
     3361 aaatgaaatt acacgcttag aaaaagagaa taaacggtta aaagaatagg tagaactcct
     3421 aaaaattacc gaagtcttcc tgaaagaa
//
LOCUS       DAZUMT010000172         3429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000172 DAZUMT010000000
VERSION     DAZUMT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.179"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2199)
                     /locus_tag="ACG8FM_002831"
     CDS             complement(<1..2199)
                     /locus_tag="ACG8FM_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFS5095416.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESLSESFNEARSSEPSITQESQIER
                     QEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVRVPRASIGLIEF
                     IRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITFEVAFFQSGGTE
                     PNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGNLGNLLIDGQDV
                     TNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREPISGGVRIAEVT
                     NPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYALEVTDVDLFNE
                     GRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNVINLNENELTYT
                     LHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRFSANNTIGGPTS
                     FTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKVIKTDENGQRLA
                     GAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYLLDATVHDVFIN
                     EYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGETLQTG"
     gene            complement(2871..3139)
                     /locus_tag="ACG8FM_002832"
                     /pseudo
     CDS             complement(2871..3139)
                     /locus_tag="ACG8FM_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          963 a          761 c          543 g         1162 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tacattaaaa gactcacttg tgctttcttc tggtacactg atttctattg agaccgtatt
     1861 tgattgaata tcaccgcttc ttgcggtcaa ttcgtattta cctgaatgct gggaagaata
     1921 agtaatcaga cgttcgtaat cattgagttg cttcacttcc ccttcaatca aatcctcact
     1981 cttatcaatg accaatgcat ttagcaggtc tggtgttcct ctgactcgca actggaactg
     2041 ctcacccaaa gcagcctcct cttgatcaac ttcgatcgtc agcgaacgct ctggctgttc
     2101 tgctaaagac tcagctagaa ccgttatgtt cccaaattga ttgatcacaa taagcataac
     2161 tagaaacata cttaacagtt tatgaaattt gttcctcact attactcccc cttataattt
     2221 tgactaaaca acaaggtacc cgataatttt ctaaaaagat tccccaactc aaaatggctg
     2281 cgttacctcc ctcgtatttt cggttcaact caagcgttgc aatgtaagct gtaaacaaag
     2341 ctaatgattg ccgattgtct tctgttgatt gattcgatag ctttgtatga cttttagttg
     2401 tccgtgtggc ttaaccataa ttatgatttc acgattcctc ctgccagtgc cttgcacaag
     2461 ctggagcgta accctaatag ttattttgtt aatgtactta tatactttta tacttttcca
     2521 ttagaaacaa tcactaaatc gttgatacca aggtcagact ctagaatttc atcagtgagt
     2581 ccctgtattg gttaaccttc atcaatccca aaaaaatata tcaatgctta gcatcaaaat
     2641 gaatatatag tttagaatac ttgggattca tcgataactg atcgcttatt tggccatatt
     2701 atttggcact tggttcaact aaacgcattt aggatgttcc ttcattctcg tcatggatgt
     2761 tctcaaaccg tcattactaa taaactgtcc accgaatcga tggttcattt cttgcatttt
     2821 ccacgtccaa ttactctttt tgctctttat ttgggcgtag tttcactttc ttaacttgta
     2881 tcgtcttcca tcaactcctt aatcatcttc ttagctttat tggctcggtt tccttgtaaa
     2941 aggtatctac aaatagtgac aatctgtatt aaatcttcat cattttctgt aatgtcaata
     3001 atttctatat tctgtaccat atattgcaaa gggtttcact aagttcataa ccgaagcgaa
     3061 ttaaacaact tttgtataga ataactacct tttctatttt tccattggta attctatcta
     3121 ttaattggtt taatcctttt gttgtagttg attccactac caagatgatt ctctcgaatt
     3181 ttaaagcttg aagatgtatt gctatttttg ctaactggcg tatttgcaac tatttttccc
     3241 ttctattctt ccttcattga ttcccctcta aaattactat ccataaaaaa ttgcataaat
     3301 gacaatagta tttatattag tcatactgct ttttaacaca gcaaattcca gaaaaacatt
     3361 cgttacatat ttgtgaattt ttccaaaaaa taacattttt ataaaacgac gaaaaataaa
     3421 tcactacca
//
LOCUS       DAZUMT010000173         3380 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000173 DAZUMT010000000
VERSION     DAZUMT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.180"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..1171
                     /locus_tag="ACG8FM_002833"
     CDS             131..1171
                     /locus_tag="ACG8FM_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5095417.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            1995..2327
                     /gene="traE"
                     /locus_tag="ACG8FM_002834"
     CDS             1995..2327
                     /gene="traE"
                     /locus_tag="ACG8FM_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS5095418.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2946..3287
                     /locus_tag="ACG8FM_002835"
     CDS             2946..3287
                     /locus_tag="ACG8FM_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS5095419.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1257 a          458 c          643 g         1022 t
ORIGIN      
        1 ttctgatttt aggtagtatt taattaaaaa acagttttaa tgcgtttgca ttttttattt
       61 atctttgcta ttttcgaaaa gagtaggtaa ttttacataa ataaaacaac gtgaaagtag
      121 aggatctaca atgactgatt ttaaattttt taaggcggat agagtttaca acgaattatt
      181 ttatcaattt ccaaaagtct ttattgtttc tgacgaatac aaaaaaatga aagattcaac
      241 taagattgcc tatatgcttt taaaagcaag attagagatc gcaatcagca aacggcaaat
      301 cgatgaagaa ggtaatgttt attttactta tacgacaaat gaactatgta gagtattaaa
      361 ctgccaaaaa caaaaagcga tagcaatcaa aaaagagttg gaatcctttg gtttattatt
      421 acaaaagcag atgggattta acaaacagtt agggaaaaat aatcctaata gactatatct
      481 agcagaatta aaagtctcag aaaatgatat ctacttactc gaaaaatttg atagagagaa
      541 tagggaaaac gttgataaat cagagggtat gaaaatcata cccaccctcg acgaaaaatc
      601 agacgctgaa tcccttgggg ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga
      661 taaatcagag ggtatgaaaa tcataccaga acttaataat aatatattag acactaatag
      721 acacaatata gacactgaaa aagaccgcct acaagatcaa ttgttgttag acaattttga
      781 gacaattatg acaaacgaca gcattgctac gtttgtccct gaacgagtat taaatttgat
      841 aaaaacattt tcttcaagtt acagtgaagc tcaaaaaacc gtccagacta ttcataatgc
      901 aaagaaaaaa gctgaaatag aaagtgatat ttcgatagtt tttgaagaac tcgatagtta
      961 ttatgtcaat gcagaacaag aattatacac gacactgtta aaagcctatc aaaaattaaa
     1021 aaccgaaaaa gtcgaaaata tccagaacct gatttttgtc tatgtaaaaa attggtttat
     1081 cgaaaaacca atagctgcta aagtatcaag tgaaaaacgt ttgaattatg caagctcccc
     1141 aagcactatt acgaaagact ggttagagtg aaactttact gacaaagtga ttgttgcttc
     1201 atggagtaac attaaaatta aaaatttttt attattctaa ttatattacg aatattacaa
     1261 aataatagct attttaatag aatttataag ttaaaatatt atgataaaaa tcacaaacgt
     1321 agccttttac gtcataagtg ttaaaagaaa taattaattt caaatataga aaagggaacg
     1381 tatgtttgca tactcctttt ttttgagtta taataatacg cgaagagaga taatttcatt
     1441 atttaaacaa gctaaaaatc ttgatattac ttcttttcag gcaatacgaa tattattcgc
     1501 ttacattttt tgtataaatt taattgtctg ctatgcttgt gatgaattga ttttcgtttt
     1561 gcacttttgg atgataaatt tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa
     1621 ctcggaaggc tggtaacctt cgtcagcgtt catacacggc attaagattt ggcccttaat
     1681 gcgttaacct ttcagtgtta cggtattacc cgcaatggat ttagtataac agatattcaa
     1741 aagtgatacc actttttttg gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc
     1801 tatgcctaag cataatgtta taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct
     1861 gaaattagag ggaacgcatg tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa
     1921 atcaattctc tatttttgaa aaataacaag aaaaacttgt taaaaatatt taaagaaatt
     1981 gggtggttta ttttatgtca tatacaggaa ttttgagtct caaagatatt tgtcactacg
     2041 gtaaacggtg taccgctaca gagaaaatca cgaagaaact atcaactggc caaagtaaaa
     2101 ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa agtctcagag gaaatgattt
     2161 attacattgg gaaacaaaag caaataattt taaaagatcc tatcccctta aaagagctat
     2221 atccaacaat caaacatgtt tatgaccaaa acggtgtact gattggtcga agaaaaaatg
     2281 gagtcttgcg ttgtaccgca aaagggatgg ggcgtttgat cggctgaatt gatcattctc
     2341 ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa
     2401 cttagagtca ctagaaaaat aaggagtaga cttgatctat caacaattcc cttcggggag
     2461 tgacggggta ggttttaacg attggtaacg taaaaaattg gtcaatcgag taaatgcgct
     2521 tgtttggata gtggtgaaat gaatttgtgt aaataataag cgcagagtaa acaagaggga
     2581 atttagaagt tagacaagtt agaatagcgt ctagattaat catgagcagc tactaaggga
     2641 tctagacttt gacgcaaact catggaataa ggagtaggtc ttctcgtaat ggggaggacg
     2701 cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac ttgcgaggac gttgattatt
     2761 ggatcaatcg agcatttacc gctgtctact agggtttctc gtttcggacg ccataaacga
     2821 gtcgaaattt tttatgtatt tttaagtaaa cgaaaaacga taaaatatga acaagtcctg
     2881 aataaagtga aaaaattgat ataaaacata gagaaaatga aatttacgta atggaggaat
     2941 gacaaatgaa aaattatgat ccgaatatac gattgggcac acatactatt aaggtttctt
     3001 tccaacgttg ggattacaaa ggatttgtga cgtttagaag gggcggaaat tgtaaaggat
     3061 tagatgtttt agcattagac gaggatgatt tatacgatca aacattgact gataatccaa
     3121 ttggattcgg cttgctacct gaagatgatg aaggtaatga atggtttaaa atgacgctta
     3181 tgaatgataa tggagacgaa ttatctgttg aagatacttg gtcatatttg agtgattata
     3241 ttgtcagctt tgaaatcatt gaatttgtag cggataaaga agaataaaaa ctggaggaat
     3301 aaattttaca gataaaggct tagaaggagt cacgacgatt aataaaaagg tagtattacc
     3361 ttaggcattg agtaaggaat
//
LOCUS       DAZUMT010000174         3215 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000174 DAZUMT010000000
VERSION     DAZUMT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.181"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..338
                     /locus_tag="ACG8FM_002836"
                     /pseudo
     CDS             63..338
                     /locus_tag="ACG8FM_002836"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            365..664
                     /locus_tag="ACG8FM_002837"
     CDS             365..664
                     /locus_tag="ACG8FM_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFS5095420.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            838..1389
                     /locus_tag="ACG8FM_002838"
     CDS             838..1389
                     /locus_tag="ACG8FM_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFS5095421.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1535..1828
                     /locus_tag="ACG8FM_002839"
     CDS             1535..1828
                     /locus_tag="ACG8FM_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFS5095422.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1821..2117
                     /locus_tag="ACG8FM_002840"
     CDS             1821..2117
                     /locus_tag="ACG8FM_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095423.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2192..3190
                     /locus_tag="ACG8FM_002841"
     CDS             2192..3190
                     /locus_tag="ACG8FM_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5095424.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1204 a          445 c          647 g          919 t
ORIGIN      
        1 ctatttttat ggcaaaggag ggaaaagaaa tgtttcaaaa taaaggataa cgttatgtca
       61 caaagggagt aatggacagt ttgccggtgg aattacaagc tttatgttgg aatctaatcg
      121 atcaaaacgt tcaaaagcaa tttcctttag actatcttca aatttttgaa ttttctacgg
      181 aaaaaggaaa tcaaaagttg gttcatcgtc aagaagaacc ggaagaaaga aaagagtatc
      241 tgatttcacc taagcttcgt ttgaaatcag tgtctcaaaa aatatgggcc attgattcgg
      301 gtgagtatca aacgatgtta ttaccagaag aatactagta tctaaaagaa aggaacgaaa
      361 ggacttgtta gaaacggcta tcgaagtact ggaaaagtgt gcacagctgg tcactgcttc
      421 tgaagagtgg ggctatgaat ccgttacgat ggaaaaagag gaaattgaaa tgggtatgct
      481 tcctgaagat gttcatctac cacgtttagt gatgacacat ctttatattt attgcgcacc
      541 agaagatgga aaagactatg tagtttattt cattacagat attaccagcc agcgagaatt
      601 tgttcgtggg ttactggtag aaggacgatt ggtttggagt cagattggag gaacggatga
      661 ataagatcga ttatttgaga atgattgcga aatgtttaga agatatcctt accgcgattc
      721 atcaaattct gaagaaaaaa ggagaaagaa tatgaaatat tttcaactag tattgacact
      781 tataacggca atggtaattg aagtgcttga acaaaaaata gcataggaga aaaagagatg
      841 attgaaaaag aattagtgac cacacaaaca gaagtttggc gagatcctca tcatacccat
      901 cgctatgttt tcaaacgaca atgggcgatc aaaggaaaag aagcggaaga taaattagca
      961 gtagtcatta ctattcgtcc aacagataca gaaccattta cgaatgattt gagtatgtta
     1021 ttaatcgaaa agaatatccg acaattagga tttactggtt ttattgctgt gaatcttttt
     1081 tcgtacacaa aagcgaaaag tcctcgggta tttcttcgag gaaatgatga acaatctttg
     1141 gaaatattaa cgactgtttt tacagaaaag aaaatcaata ccattatttt tgcttgtggt
     1201 tcgattacag cgacgagtca agtggcttat gaacaagcaa agactattta cgatcaattg
     1261 agtgcgaaac aaaagaagat gaccaaagta ttgataaatg cagaaggtaa gttatcccac
     1321 ccactaagta ttcatgttcg taatgaatgg caattggcag atagtcaatt attatttcaa
     1381 gaaaaataac attttatttt ctcatgttac ttataagtag ttttacatgc tttttgactt
     1441 taaaaataca tgtgatgata catgttatat gaattatcaa tgtacccagt aatatgctat
     1501 tttcattatg taaaaaataa cagggggtgt gtagatggag attgatgaaa cttgtttgag
     1561 taactcttat aaaagtatga atgacttttt tgaggaaatg gaactggatt cagaattgct
     1621 ctatcaattt tatcttgctt ttcgagggca acagatttct tttccaatga aaatgtacga
     1681 tcgtgagtta gttagaaaaa gaatcgaaaa catgatagaa caggaaaaaa ctgtagatat
     1741 ccgtcaattg acagaagttt atggtttctc tgcaagatgg attcaaaaag taattaaaca
     1801 aaaagaaagg agacgagtac atggctaagc tgattgtatt aggtggttta atttacggtg
     1861 gatattggtt atatcaatat atgcttgcgc atccgaatat agataaagga aaagtaatta
     1921 agacagttgg ggtaatcata gttggactca tagctctaac taagaaaaat aatactaata
     1981 gcccaaaaat aatgagaact tatcgctgta gaggatgtgg aagggaaatt tctactagtt
     2041 ttaatccatc tgagagtcct caaggaagag accgctgcca tacgggctat cgacattatt
     2101 gggaaagaat cgattaaaag attaccattt agatcaatta attgtctgat agaataagta
     2161 cgtgagggag aaattgaaag ggggaaatat aatgaaaaaa tggctgttat tactaatagg
     2221 aatgggaatt ttattcactg gttgtacatc tgagaatacg aggactaaag cgactataga
     2281 aactgataca acaattgaga ctaaaaaaag taaaacgcaa tcaactgaac aaactacaac
     2341 agaaatgaag caatctgtgg aaacaagtgc aagttcaatc actgaatctt cttctgaaga
     2401 ggttaagaat actttgtggg atacgaataa agcttccaaa ttagaaactt ttgtcacaca
     2461 atggggaaag acattaggtc aagaatataa aagctacaat cttcagaata atgtaagtct
     2521 gtatggcact cctttaccac aagccgtgat taatggcgat tggaaaatgg caatcaatga
     2581 agcaccagtt accgtacaat ggtcagaaga tggaactggt catgcagatt tcaatttagt
     2641 agcagtttat tctgatgtag aaactggaga atatttaggg cagcatgttt actttttcgg
     2701 ttttcaaaat ggacaaccta aagtttattt aacgcaacaa aatcaaggta atgaaaataa
     2761 ttatctgtac tttaacgaaa ctcaaaatca acagttaaaa caaggtttca gtgatattgt
     2821 gaatggacaa actcctcaaa caccagtagt agaagaatct actcaacaag caacaactac
     2881 aacgacacaa tctacaagta gttcaggaat ttcaagttgg gaagctgcta aagaatctgt
     2941 gctaaataat aaagctttat ggttgtatga ttctcctgat aaaaatgtat caaatactaa
     3001 tttaattagc acaaatgaat atatgaaaga tctaatgcaa gatgaaaatg gaagctacta
     3061 ttcaattaat attgtaacgg atgatgatgg aaacacgacc atggctggtg gaaaacaatt
     3121 tcgtgtttat acagatggaa gaatttctca acgtattgga atgactaaca attttcaaat
     3181 gattaaataa ttgaaaatat tatttatcaa agaaa
//
LOCUS       DAZUMT010000175         3199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000175 DAZUMT010000000
VERSION     DAZUMT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.182"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8FM_002842"
     CDS             301..585
                     /locus_tag="ACG8FM_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095425.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8FM_002843"
     CDS             588..1004
                     /locus_tag="ACG8FM_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095426.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8FM_002844"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8FM_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5095427.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG8FM_002845"
     CDS             2522..2608
                     /locus_tag="ACG8FM_002845"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5095428.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..>3199)
                     /locus_tag="ACG8FM_002846"
     CDS             complement(2910..>3199)
                     /locus_tag="ACG8FM_002846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095429.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAZUMT010000176         3176 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000176 DAZUMT010000000
VERSION     DAZUMT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.183"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..712
                     /locus_tag="ACG8FM_002847"
     CDS             <1..712
                     /locus_tag="ACG8FM_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFS5095430.1"
                     /translation="TLSRNKKYIENETDSVVLVSSEKVIDICENKILTSAFFEQNGFK
                     TPEILNSSEITKFPVFIKPLNGSSSVNTFIVNNEKELLFFSEYIDNPLIQEYILGEEY
                     TIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIKDRELIETTKKMLEKLNAIGHITI
                     QCIKNEKGIFYLEINARYGGGAPISIKSGANSPEYIIKMIDSKKNLRYRENYLDKMIA
                     LRYDQAIYLDEKGNVYND"
     gene            705..1400
                     /locus_tag="ACG8FM_002848"
     CDS             705..1400
                     /locus_tag="ACG8FM_002848"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5095431.1"
                     /translation="MIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIFPATFDEI
                     YRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLENADTLLTE
                     LRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLE
                     KFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIR
                     SLWEIKNFLGVIS"
     gene            1397..2515
                     /locus_tag="ACG8FM_002849"
     CDS             1397..2515
                     /locus_tag="ACG8FM_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFS5095432.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            2505..3125
                     /locus_tag="ACG8FM_002850"
     CDS             2505..3125
                     /locus_tag="ACG8FM_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5095433.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT         1213 a          394 c          596 g          973 t
ORIGIN      
        1 aacattatca agaaataaaa aatatattga aaatgaaaca gactcagttg tattagtttc
       61 ttcagaaaaa gtcatcgaca tttgtgagaa caaaatttta acttctgctt tttttgaaca
      121 aaatggtttt aaaactcctg aaattttaaa ctcttctgaa attacaaaat ttcctgtatt
      181 tataaaacct ctgaatggta gttcatctgt taacacattt attgtaaata atgaaaaaga
      241 attgttgttt tttagtgaat atatagataa tcctcttatt caagagtata ttttaggtga
      301 agaatatact atagatgttt gcttaaactt tgattcgaca ccagtaagta ttgttcctag
      361 aaaaagaata gcaactagag gtggggaaat actgaagggt cagattatta aagatagaga
      421 actaattgag actacgaaaa agatgttgga aaaattaaat gcaatcggac atattactat
      481 tcaatgtata aaaaacgaaa agggaatatt ttatttagaa ataaatgcaa gatacggtgg
      541 aggagccccc attagcatta aatcaggagc taattcccct gaatatatta taaagatgat
      601 agattcaaaa aaaaatttga gatacagaga aaattattta gataaaatga tagcactgag
      661 atatgatcaa gcgatttatt tagatgaaaa aggaaatgta tataatgatt aaagcggtga
      721 tctttgattt agatgacact cttgtgtctg aatatgatta tgtaaaaagt ggatacaaat
      781 gtgtagctag aagattagca gtgcaaaaaa ttttcccggc tacttttgac gaaatatata
      841 gagtcctttt agatcttttt gctgaagata gtaaaaatgt atttaatcgt ctatatgact
      901 attttgaaat tccttatagt aaaaaagaaa ttaaagaact ggtaaatgta tatcgagaac
      961 acaaaccaga cataaatttt ttagaaaatg cagatacttt attaacggaa ttaagaagta
     1021 gaggatacaa gctggggatt attacagatg ggtatgctat aacccaacga aacaaattgg
     1081 atgctttgaa agtagactat ttggtagatt atatttgtat tactgacgaa ttaggccgtg
     1141 aattgtggaa accacaccct atctcatatc ttttattgtt agaaaagttt aatttaaagg
     1201 cacaagagtg tatatatatt ggcgataatg agttgaaaga ttttataact gccaaatcaa
     1261 taggaatgca aacaattaaa atagaaagag taaatggtat ttacagagaa agcagtttga
     1321 agttcaaaag attttaccaa gctgataaaa aaatacgttc gttatgggag ataaaaaatt
     1381 ttttgggagt gatttcatga aaagtaaaag aattctatta gcttctccac atatgagtga
     1441 agaaggatat gaaaaagagt atgtaaaaga agcatttgat acaaattggg ttgctccttt
     1501 aggtgcgaat gtgaatggat ttgagaaaga attagcagaa tacgtgggag ctagtcatgc
     1561 tgccgcatta tcttctggaa cagcagctat acatttagct ttgaaagcgg ctggggtcgg
     1621 taaagatgac atagtgattt gtcagtcctt gacgttctca gctactgcta atccaattct
     1681 ctatcaagga gctatacctg tatttgttga tagtgattac gaaacttgga atatgaaacc
     1741 tgaacatttg cgaacagcac tagaaaaata tcctaacaca aaggctgtat tagtagtaca
     1801 tttgtacggt ttatcagcta atttagacga aattactaaa atatgcaaag aatacgatgt
     1861 tactctaatt gaagatgctg cagaatcatt aggaacaaca tataaaggaa aatatactgg
     1921 tacatttggc gactatggaa tttattcttt taatggaaat aagataatca caacttcagg
     1981 tggcggaatg cttgtatcca atgacaaaga aaaggtggaa aaagtgagat tttgggctac
     2041 gcaatctcgt gagaaggcac gccattatca acacaatgaa attggctata attatcgaat
     2101 gagcaatatc gttgctggta tcggtcgagg acaattgaaa gtattaaatg aaagaattga
     2161 gaaaaaacga tatattcata gtttttataa aaaagaattg tctaaattgt caaatataca
     2221 attcatgcca gaaaataatt ggaatgaacc gaattattgg ttaagtgtca taacaataaa
     2281 gggaataaat gcgaatcatc ttattgatga gttagacaaa aataatatag aagcccgccc
     2341 agtatggaaa cctatgcatt tacaacctgt ttttaaaaat tatgattata ttggcgatga
     2401 tgtagcagaa actttatttg acttaggagt atgtttacca tcagatacaa agatgacaga
     2461 tgaagattta aaatatgtag tatctacgat taaaagagtg atcaatgatg cgtaaaaaaa
     2521 ctttctatca gctttttggg aaaagaattt tagatatcct attgtcagga attgctctca
     2581 tcgttttatc gccgataatc ttaattgtag gttttttagt aagaattaaa cttggttctc
     2641 caattatctt caaacaagaa cgaccaggaa aaagtgaaaa aattttttcc atgtataaat
     2701 ttcgtacaat gactgacgaa agagaccaca atggagaata tttacctgat gaaattcgac
     2761 taactaaatt tgggaaaatg cttagagcaa caagtctgga tgagctaccc gaactttgga
     2821 atattttgaa aggtgatatg tcgatcgtag gacctcgtcc attattagtg gagtatttgc
     2881 cactatatag tgagaagcaa cggaaaagac acaacgtaag accaggatta actggactgg
     2941 ctcaggtcaa tggaagaaat gctattagtt gggaagaaaa gtttgattta gatgtttatt
     3001 atgttgataa aattagtttc tttaatgatc ttattattat aattcagaca tgtaaaaaag
     3061 taatcaagaa agagaatatt aatacaatca ataacaatat cccagaaaaa tttaaaggga
     3121 gttaaataca gcagcttaat gcaagttttg tgacttgcat tatatttagg tataaa
//
LOCUS       DAZUMT010000177         3097 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000177 DAZUMT010000000
VERSION     DAZUMT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.184"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..822
                     /gene="fabI"
                     /locus_tag="ACG8FM_002851"
     CDS             67..822
                     /gene="fabI"
                     /locus_tag="ACG8FM_002851"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS5095434.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(932..1984)
                     /locus_tag="ACG8FM_002852"
     CDS             complement(932..1984)
                     /locus_tag="ACG8FM_002852"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS5095435.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2110..3030
                     /locus_tag="ACG8FM_002853"
     CDS             2110..3030
                     /locus_tag="ACG8FM_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS5095436.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          899 a          624 c          590 g          984 t
ORIGIN      
        1 ttttagctat tattctctat ttttttacga ctatcaaatt tttatcagaa acaaaggaga
       61 gtcattatgt catttctaga aggaaaaaaa atcgtcatta tgggcgtcgc taacaaaaaa
      121 agcattgctt ggggttgtgc tgaagcaatg ttgaaacaag gggcagaaat tatctatact
      181 taccaaaatg aacgaatgaa aaaatcatta gtcaaattgg tcggcgaaga agcattcact
      241 gtagaatgtg atgtttctag cgacgaaagc atcgaaaaag caatgaatgc catcggtgaa
      301 tatgctgggg agatccacgg ccttgtccat gcagttgctt acgccaacaa agaagaattg
      361 tccggaaatg tgtctgatat ttccagagac ggctatcaat tagctcaaga tatcagcagc
      421 tactctttaa ttgctgtatc aaaatacgca caaccgtttc ttgcaccaaa cagcgggatc
      481 gttagcatga cttatctagg ttcagaacgc gcaatcccga attataacat gatgggtatt
      541 gcgaaagctt ctcttgaagc tgctatccgt tatttggcag ctgagttctc gcctaaagga
      601 attcgggtca atggaatctc agctggggca atcaaaacat tagctgtcac aggtgttaaa
      661 gactatcaaa aactgatcca gttatctgaa tctcgtacgc ctgatcagca aggtgtgacg
      721 atcgaagaag tcggtaatgc ttgtgctttc ttaatcagcc cactttccag cggaatcatc
      781 ggtgatatta tctacgttga caaaggtgtc catctttcat aaacttttgt tgcattcttt
      841 aaagtaaaac atataaaaat caggaaaaaa ctttttaaag tctcttcctg attttttctt
      901 tttacgtttt tttcaactca ttactttgaa attattccac gacttcaatc cagccttctg
      961 gtgcttctat gtctccaaat tgaatacctg tcaattcatc atacaaccgt tttgttactg
     1021 gtcctacttc cgtttcacta taaaatacat gaagattgtt attgtgataa atcccaccga
     1081 ttggagaaat aacagccgct gtaccacatg caccagcttc tgcaaattga tctagatcat
     1141 caatgaaaat atctccttca attgcttgca gtcctagtcg ttcttcagct aactgcaaca
     1201 aggaatattt cgtgatactt ggtaagatag agggcgattg cggagtgatg aattttccgt
     1261 cttttgtaat tccaaagaaa tttgctgcac ctacttcttc gattttcgta tgtgtggctg
     1321 gatcaagata aacacaatct gagtaatcca gttcttttgc ttctgttcct ggaagtaaac
     1381 tcgctgcgta atttccgccg acttttgctg caccagtccc cttaggagca gcacgatcgt
     1441 aatcagatac aataaaattc gttggtttta gtccaccttt gaaatacgct cctactggcg
     1501 tacagaagac gacaaataaa tattctggag ctggatggac accaatgttt tcaccgaccc
     1561 caatcagtaa agggcgcaag tataatgtag cgcctgttcc atagggcggc acaaaatctt
     1621 catttgcttt taccacttgc ttgacagcat caacgaacat cttttcaggg accattggca
     1681 tcagcaatcg tctagcactt tgattcatcc gtcgactatt ttgttcaggt cgaaagagat
     1741 tgaccttgcc gtcttttcga cgataagctt ttagaccttc aaaacattgc tgaccataat
     1801 gaagcgctgg agagccttca tgaatcgtca ggtaattatc ttctgtcaat tctccttctt
     1861 cccattgtcc gtctttccat ttggcaataa aacgccaagg agttttgata taagaaaaac
     1921 ctagattgtt ccaatcgatt tgcatatact taccaccttc gttattttct ccacatttta
     1981 tcattatcca tattatttgt cattttattt tcagcttttt tccttcatta tagttatttt
     2041 tgctatttcc ttttttgaaa acttcttggt acaatacaga aagaatcaaa ttttagaaaa
     2101 gaggatttta tgttttcatt attagcaact gccggtacga cagactcttc tggcgagaca
     2161 ttgacagatc aagctgtcag caaagtaaat gcgtttcagc ggttttggga cgggattgat
     2221 tgggacgcca ttttggctgc ctttatccaa aaaagtttat atctgatttt tttgatcgtc
     2281 cttttttgga ttcttcaccg tgtgggaaaa tatctgattg ataagtcttt taatcagtat
     2341 tctaaaaaag ccattctaaa cgagagccgc ttaaagactt tacatagtct tttaaatact
     2401 atttttcatt atacccttgg tttctttttt atctatgcaa ttttatctgc tatcggtgtg
     2461 ccgatcggat cacttttggc tggtgccgga attgcaggtg tggcaatcgg tcttggtgcc
     2521 caagggttca tgagtgatgt tattaccgga ttttttatta tcatggaaca acagatggat
     2581 gttggggact acatcaagct agcaaactta acgattgaag gaaccgtcgt atctgttggt
     2641 atccgtaccc ttcagctgaa agcaacggat ggtactgtcc atttcatccc gaatcgaaac
     2701 atcaccacga tcagcaatct ctctcgtgca aatatgcaag tacttttaga tattcggatt
     2761 gttcctgaag aaggatatga caaaatctat gagatcattg atcgagtaaa tcgtacatta
     2821 gctgaaaaat accaagaaga gctccaaact gaaccttcta tctttggatt agtggatctt
     2881 ggaaacagca attttgcgat tcgtacggtc tgctacgcat tgaacggaaa acaatatgcc
     2941 ttaaaagaag agttcttgag ctcctatgtc aaagaattga cagcttctgg tttcacgatt
     3001 ccgaacagtc caattgctgt cggaaaataa tttggacaaa cgatgcaacg aatctcacta
     3061 aaaagagcgt gggacaaaag taaagaacac ttttgtc
//
LOCUS       DAZUMT010000178         3090 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000178 DAZUMT010000000
VERSION     DAZUMT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.185"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(307..921)
                     /locus_tag="ACG8FM_002854"
     CDS             complement(307..921)
                     /locus_tag="ACG8FM_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5095437.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1527..1904)
                     /locus_tag="ACG8FM_002855"
     CDS             complement(1527..1904)
                     /locus_tag="ACG8FM_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095438.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2204..3085)
                     /gene="galU"
                     /locus_tag="ACG8FM_002856"
     CDS             complement(2204..3085)
                     /gene="galU"
                     /locus_tag="ACG8FM_002856"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5095439.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          936 a          646 c          477 g         1031 t
ORIGIN      
        1 atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc
       61 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
      121 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
      181 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
      241 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
      301 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
      361 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
      421 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
      481 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
      541 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
      601 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
      661 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
      721 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
      781 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
      841 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
      901 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
      961 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
     1021 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
     1081 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
     1141 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
     1201 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
     1261 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
     1321 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
     1381 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
     1441 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
     1501 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
     1561 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
     1621 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
     1681 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
     1741 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
     1801 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
     1861 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
     1921 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
     1981 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
     2041 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
     2101 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
     2161 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
     2221 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
     2281 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
     2341 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
     2401 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
     2461 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
     2521 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
     2581 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
     2641 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
     2701 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
     2761 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
     2821 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
     2881 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
     2941 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
     3001 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
     3061 gaatgactgc ttttttgacc ttcacttgct
//
LOCUS       DAZUMT010000179         3035 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000179 DAZUMT010000000
VERSION     DAZUMT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.186"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..1580
                     /locus_tag="ACG8FM_002857"
     CDS             102..1580
                     /locus_tag="ACG8FM_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS5095440.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1648..2739)
                     /locus_tag="ACG8FM_002858"
     CDS             complement(1648..2739)
                     /locus_tag="ACG8FM_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5095441.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         1074 a          528 c          471 g          962 t
ORIGIN      
        1 tttaatcaat aaaaaaattg acagttttat taataataag ctattctgtt atagaagaaa
       61 aaattgtttg ttattattag caacaaaaaa aggagtgcat catgaaaaag ttatctcaaa
      121 aacgtttagc tcaaatcatt attgattcta gaaaatctct gaaaatgaca caacaacaac
      181 ttgcagtagc tactggtatc aaccgtgcct taattagtcg attagaatct ctcgatttta
      241 tcccgtcaat acaacaatta gaatcattag cagaagttct aaattttgaa ccaacagatc
      301 tatttataaa tgaatcaaat aaatcaaaca gaacaaaaca tcagtcgaag aatattgctg
      361 tagccggtac tggttacgta ggtctttcca ttgctactct tcttgcccag cacaatcagg
      421 taacagcagt agacattctt cctgaaaaag taaaacaaat taacaataag aaatctccaa
      481 tacaagacga ttacattgaa aaatatctag ctgaaaaaaa tcttaattta attgcaacga
      541 ctgatggtgc agccgcctat agagaagccg attttgttgt cattgctgct ccaacaaatt
      601 atgatagtaa acaaaattat tttgatactt ctgctgtaga gtcagtaatc aaattggtgt
      661 tagaagtcaa tccaaaggca ataatggtca tcaagtcgac ggtacccgta ggctataccg
      721 aaagtattcg taaaaaatac aatactaaaa atattctatt cagcccagaa tttctcagag
      781 aatctaaggc attatatgac aatctatatc cttcgcgaat tattgtcggc acggaccaaa
      841 ctgacaaaac tttacgagaa gcctctcaca cttttgcaga tttactccaa gaaggagcaa
      901 tcaaaaaaga tatcgaaact cttttcatgg gattcacaga agctgaagca gttaaattat
      961 tcgctaatac ttaccttgca cttcgagtat cttacttcaa tgagctggat acttatgcag
     1021 aaatgaaagg actaaataca caagaaatca ttcacggggt ttgcttagat ccaagaattg
     1081 gcacacatta taataatcca tcttttggat atggaggcta ttgtttaccg aaagatacaa
     1141 aacaacttct cactaactat aacgatgttc ctcagaatat gataagtgca attgtcgata
     1201 gtaatcgtac aagaaaagac tttatagcag atcgagtgtt agaacttgct ggcacgtatg
     1261 aggctaacag ttcgtggaat gcagaaaaag aaaaagaggt agtcataggt gtataccgtc
     1321 ttacaatgaa gagtaattca gacaacttcc gtcagtcatc tattcaagga gttatgaagc
     1381 gaattaaagc aaagggggca actattgtaa tttatgaacc aacacttgaa aataacacta
     1441 cattttttgg ttcaaaagta ataaataatt tagatgattt taaaaatatc agtcacagta
     1501 ttattgctaa tcgttacgat agtaatcttg atgatgtaaa agaaaaagta tataccagag
     1561 atttatttca acgagattaa aaaataacac ctcagatttg tagtgtctga ggtgtttcta
     1621 taaactttta taagtaaaaa aatacaatta tacttcataa aaagacttat accactgcgc
     1681 aaatttacga agcccgtcac gcaaacttgt acttggttta aattcaaaat ctcgttctaa
     1741 cgcactagta tctgcataag taactggcac atctcctggt tgcattggaa ctagttcggt
     1801 atgagattca aagtcgtaat cttctggtag cacccctgct gcaattagct cttgttgtag
     1861 aatatctaca aaatctagta ggttttccgg cgtattgtta ccaatattat aaatatgata
     1921 tggtgggatt ggtagcccat cttccccatc aattttctct ggagcgcact gcataaccct
     1981 ctttactcct tcaacaatat catctatata ggtaaagtca cgcttgcaat tcccataatt
     2041 gaatatttgg atattatcac cagcacgaag tttatcagta aaaccaaaat aagccatatc
     2101 tggacgccca gcaggaccat atactgtaaa gaaacgtaac cctgtagacg gaatattgta
     2161 aagtttagaa tatgcatgtg ccattgattc attacttttc ttagttgctg catacaacga
     2221 tacttgattg tctactttat cctctgtaga ataaggaatt ttcttattag taccataaac
     2281 ggaagacgat gaggcataca ctaaatgttc cacaccagat tctccattat catatgaatg
     2341 acgacaagct tcaagaatat tatagaatcc tattatgtta gattcgatat acatatctgg
     2401 attagtaatt gaataacgga ctcctgcctg agctgctaaa ttcaccacga tacttggttt
     2461 ataattatca aagatgaaat caattgttgc tttatcagca atagaacctt tgataaaatt
     2521 ccaagtagat cctggatggt tctttgcctc cgtttctatc tctgataatc tatagttctt
     2581 aatagagaca tcatagtaat cattcatatt gtcaatgcca atgattttta ttgatgactg
     2641 agtacgtaat aactcaagaa ccaaatttga tccaataaaa ccggctgcgc cagttattaa
     2701 tattgttcta tttgataagg acgtgtttaa ttccagcatt ttttagcact cactttcttt
     2761 ttttcttatg aatacatatt tgttatatat taaaaaatct tatttagact tatctattgt
     2821 cacttttatc attctaatct ttgcacgtag tagaataatt atacagatag gaaattgcta
     2881 agcaacccac ttctttctat tatctattaa cttattctaa aacgaataga aattattctt
     2941 gatcacataa gtaaccttaa aagaaaaaat ttaatatatg tgtctaattt tggcaaatat
     3001 aattttatta agtttattaa aacatttatt gtgta
//
LOCUS       DAZUMT010000180         2955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000180 DAZUMT010000000
VERSION     DAZUMT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.187"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..407
                     /locus_tag="ACG8FM_002859"
     CDS             90..407
                     /locus_tag="ACG8FM_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5095442.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(498..863)
                     /locus_tag="ACG8FM_002860"
     CDS             complement(498..863)
                     /locus_tag="ACG8FM_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095443.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1071..1553
                     /locus_tag="ACG8FM_002861"
     CDS             1071..1553
                     /locus_tag="ACG8FM_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095444.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1750..2640
                     /locus_tag="ACG8FM_002862"
     CDS             1750..2640
                     /locus_tag="ACG8FM_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS5095445.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1009 a          460 c          566 g          920 t
ORIGIN      
        1 tttttaaaac atcacaagga aaaacttaac tatggtatta tctaaacata attttttcgg
       61 tatttaatat ttaattagga ggtaatattt tgctttttta tagtaatttt atcttaatag
      121 tagcgatatt attacttctg aatatatgga tttttgatag gagtagaaat tcaagtatag
      181 gttttagaac taaacgaagt ttgtcttcta aaaaaaattg ggtatactca caaactattt
      241 tttatggagg aattgtttta attagtttac tttcttcaac actttattct ttgaatatta
      301 tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct gcaattatta
      361 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
      421 agttttggtg atataagaaa aacatatatg tataaaaaga agaaagtagc ttctctttgt
      481 acatatatgg tttgttttta tggatgccga gcttttttat aagataaggc taccgcccat
      541 aacgacccaa ggatttgtgg aactaagtaa atacctgcaa gaagcaagat cagctgggtc
      601 ggttttaaag gatgatagaa gtaacgccat tcagtaaaat tggcgcaaaa taaataggaa
      661 gtgacagtag atataatatt ggcaatgaat aaagatataa tacgcctgtt cctttgaaaa
      721 taatatccag caaaaagaga taaaaatatc aatgtgatga acccgcttac ggaatgataa
      781 cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa aagataaaag caacggaagg
      841 tttttccagc aagtttttat catctttcat caccacctaa caagtgttat tattttgtta
      901 gttttattgt aaattgaatc ctgcaataat caattacttt ttcattattt ctagattaaa
      961 tgaataaact ctcatgccat tatctctaaa aaaaaagttt ttccccctaa tttttttaga
     1021 tagaaaattg tatagtatga tgagagaaaa agtagtttgg gggaacgtta atgagaagaa
     1081 aagcagtaat cgatcggctt tatttatttt tttggttttt tcctatcgtt ttgctacctt
     1141 ttacatgggt aaatgctgtt gtacaaggtg cggagatatt aggcctgatc caaggaagcg
     1201 gatttgtggt ttctaggaat tttccagctt tgattgtttt ctatttagtg ataagtgtag
     1261 gtctcttatt accaaaatct ctgatcaatc actacaattg gcagctgctt tacttttctt
     1321 gcttattagt atttacatct acgttttctt tgctgcctcg cttgattatc ggccctaatg
     1381 gtttgagcga gagtggattg aaggatttag cttattcctc catgtatttc ttgacgatca
     1441 aaccatggtt ctatttagta attgcctcat ttattcttgc aacatttttt tacttctgga
     1501 cagaaatcag gataaaaagt gaacgatctg tatctataaa aaataactat tgatggtgga
     1561 tcatcagtaa aaaaacatat gagctgcgta aaatgcatgt ttctcatatg ctttttttat
     1621 tattatgtaa agaatgcgaa aaacgtttac atgattttta cataacttaa ctatattttt
     1681 acagcaacca tacgagggat ttacagtcga ctgataaact ttagttgtaa ataagaaagg
     1741 agcaataaaa tgaaaaaagt attgtttact ctaggtatga tgggaattct tttagccgga
     1801 tgcggctcag gatcaggaaa tacagatggc tctgccacac aaaataattc aagtgactca
     1861 aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat tacagccgtt agtcgatgca
     1921 gcacaagaaa gctttgtaca agaaaatcca aattatcaaa tctctgtaca aggaggagga
     1981 agcggaactg gtttgagcca agtcgaagct ggagcagtta ctatcggaaa ctctgatgta
     2041 tttgcagaag aaagagacgg agttgatgct tcaaaattag tggatcataa agtagcagtg
     2101 gtcgggatgg caccaatcgt caataaagat acagatgtca aagatatcac caaacaggaa
     2161 ttgattgata tctttacagg aaaaatcact aactggaagg aagttggcgg gaaagatcaa
     2221 aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc gcgcgacatt cgaaaaatgg
     2281 ggactggatg gtgctacccc tgtgcagtcc caagaacaag attcatcagg aacagttcgg
     2341 cagctggtaa gtcaaacacc aggagcaatc agctatttag ctttctcata tctcgatgat
     2401 tccacacaag cactaagtat cgacggtgta gaaccaaaag aagagaatgt agcagataac
     2461 agctggggaa tctggtcata tgaacatatg tacacgaacg gaaaaccttc ccctgaagtc
     2521 caaaaattct tagactacat gatgacagaa gaaatccaag aaggacctgt caaagaattg
     2581 ggctatttac caatcacaat gatggaagta gaacgtgatc acgaaggaaa tatcaaataa
     2641 ctagcagata aacatgccac ggtagtcgtt actattaagg aaaaatcaac tatgtttccc
     2701 cacatataca tttttccttt aattgtgaaa aatagttcct catattttgc cgttttttga
     2761 gctaataccg agggtcagct gattgaacac cgtttattcg aaaaaaagag ctacgacata
     2821 gaactgtcgt aacttttttt ggctcttcgt caaatcgtgt tggtgaagac aaatttgaat
     2881 aaaacttaga ccgatatgcg gcgcgttctg tgcgccgcat atttttttag ttttcaaaga
     2941 tcagaccttg ttgta
//
LOCUS       DAZUMT010000181         2884 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000181 DAZUMT010000000
VERSION     DAZUMT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.188"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            616..>2884
                     /gene="esp"
                     /locus_tag="ACG8FM_002863"
     CDS             616..>2884
                     /gene="esp"
                     /locus_tag="ACG8FM_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS5095446.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1047 a          428 c          561 g          848 t
ORIGIN      
        1 tattcatgta gagtactaga cactttaaat atggacaatt caattataaa gaaaaagaat
       61 caataattta atgaggctgt gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa
      121 aatgttgata actccaatca ttcgtttaag attctaggaa tcattcagga atgctgtgaa
      181 accgatactt tataattata aagaaggtga tttcaaattc cgaagaagga atttgaagtc
      241 acctttttac ttatgtcaca gtctcattca gtaatgcggg ataaggctcc ttattccgct
      301 atttgaatac tctgaagttt aatctaagaa agagtaatat tttgaaaaca tgcttcatcg
      361 catagaaatg aacttaattt cagaaagaat acatacatat ctttttatat gttttcagct
      421 gctgaatggt taaaaagcac aaatatccga aaaaaacatg aagaaatgta ctttttttgt
      481 tgtaatgtgc atgaaattct ttaagtaaac tagttaacat aagtttagta agattcctat
      541 tctgtgaata atacgtatca gatttatctt ttattcttac taagaaaata actattaagg
      601 gagttgattt gaataatggt tagcaagaat aataagagag tatttctaga aaaaacaaag
      661 aaacgagtat tgaagtacag tatcaaaaaa ttaagtgttg gggttgcatc tgttttggtt
      721 ggtgttggtc ttgtcttggg aacaactgaa ctagttcaag cacaagatga aatttctcca
      781 agcaccccac ttgaaacagc gataagctcc gtacaagtag gtgacaaagt agcttcgggg
      841 aatacttttc aggagaatcc tggttatacg aaaaattata acttttcgga tttacaattt
      901 agtcctcaag aactaactgg agacacacta aaaggcaata caattggatt tgaagtttac
      961 ggaaaacata atattgcagc atcaactaaa aactgggaaa tccgtcttca attagatgaa
     1021 cgattagcta aatatgtaga aaaaattcag gtcgatccaa agaaaggaat tggatcttct
     1081 agacggacct ttgtaagaat taatgattcg ctgggtagac ctacaaatat ttggaaggtc
     1141 aattatattc gtgcaagtga tggattattt gctggggctg aaacaactga tacacaaact
     1201 gctcctaatg gggtaattac gtttgaaaag agcttagatg aaatttttaa agagataggc
     1261 atagacaatc tcaagaccga tcgtttaatg tatcgaatct acttggtaag tcatcaagac
     1321 gatgataaaa ttgtaccagg tattgatagt acgggctact ttttaacaga ttcagatgat
     1381 ttctataata gcttagatgt atctgaaaat aatcctgacc agtttaaaca tggatcagta
     1441 agtgcaaaat atgaggaacc taatactcaa acaaaagatg gttcaggatc cacaggtgct
     1501 aatggagcta taatcttaga tcataagtta acgaaaaatt ataatttctc atattcagct
     1561 tccgcaaagg gcacgccttg gtatgctaat tacaaaattg atgaacgatt agttccatat
     1621 gtagcgggta tacagatgca catggttcag gctgacaaag taacatatga tgtttctttt
     1681 gaatcaggga aaaaagtggc tgatttagca attgaacggc gcaaagatca tgaaaattat
     1741 ggtgtaggtt caattactga taatgatctt accaaactta ttgattttgc taatgcaagt
     1801 ccacgtccag tcgttattcg atatgtttta caattgacta agccattaga tgaaatctta
     1861 gaagatatga aagcaacagc acaagttgaa gaaaataaac catttggtga agatttcatc
     1921 tttgattctt ggttgtcgga tacgaataaa aaattaatcc agaacactta tggaacaggt
     1981 tattattatt tgcaagatat tgatggtgat ggaaaccctg acgataaaga agagagcgga
     2041 gacacgaatc catatatcgg gaaacctgaa ttagaagaag tatatgatgt tgacacaaca
     2101 gttaagggga aagtattcat ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt
     2161 gataaagaag gtactgtatt agcagaaaaa actatcgctc caaatgaaaa agatggggct
     2221 ccaatttcag atactgtaga atttgaattt acgggtgtag attcaagtaa attaatcgcg
     2281 aaagatgaat taaaaatcca aatcgtttct ccaggttttg ataaaccaga agaaggttca
     2341 accgttatta aggaatcacc aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa
     2401 ccagatgcta aagaatcaat tcggaataat aaaaacttac ctgaagatgc agagtattca
     2461 tggaaaacag agcctgatac ttctaacgtt actgatagta cgaaaggtat tgtaactgtt
     2521 aagatcggaa atcgaacttt cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct
     2581 atggaaaatg atgcaacata cgtacctata acaacaactc cagaaacgac agttcaaagt
     2641 ggaaaaccta catttgataa accagatgtt cctttagcta aagatgcctt ttcaatttta
     2701 gatgtctata ataaggactt cggtaatgca agtgttgatg caaatactgg tattgttaca
     2761 ttcactccag ctaaaggtgt aggagaatcg gagccgatta ctggaacaat tcctattaaa
     2821 attgtttacc aagatggttc tgtaggcacg accgatttag cagtaactgt aagtaaagat
     2881 attt
//
LOCUS       DAZUMT010000182         2823 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000182 DAZUMT010000000
VERSION     DAZUMT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.189"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8FM_002864"
     CDS             complement(296..1024)
                     /locus_tag="ACG8FM_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095447.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8FM_002865"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8FM_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5095448.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
BASE COUNT          896 a          426 c          499 g         1002 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggt
//
LOCUS       DAZUMT010000183         2755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000183 DAZUMT010000000
VERSION     DAZUMT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.191"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG8FM_002866"
     CDS             136..309
                     /locus_tag="ACG8FM_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095449.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACG8FM_002867"
     CDS             949..1422
                     /locus_tag="ACG8FM_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS5095450.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACG8FM_002868"
     CDS             1431..1658
                     /locus_tag="ACG8FM_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095451.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACG8FM_002869"
     CDS             2294..2665
                     /locus_tag="ACG8FM_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095452.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          883 a          441 c          407 g         1024 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtga
//
LOCUS       DAZUMT010000184         2742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000184 DAZUMT010000000
VERSION     DAZUMT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.192"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..1128)
                     /locus_tag="ACG8FM_002870"
     CDS             complement(34..1128)
                     /locus_tag="ACG8FM_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS5095453.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1317..2078
                     /locus_tag="ACG8FM_002871"
     CDS             1317..2078
                     /locus_tag="ACG8FM_002871"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5095454.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="ACG8FM_002872"
     CDS             complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="ACG8FM_002872"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5095455.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          859 a          546 c          465 g          872 t
ORIGIN      
        1 ttctagcgga ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa
       61 gcttgaccat ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac
      121 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
      181 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      241 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      301 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      361 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      421 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      481 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      541 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      601 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      661 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      721 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      781 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
      841 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
      901 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
      961 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
     1021 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
     1081 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1141 tcctgcatct tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct
     1201 acaccttttc tttttctttt aaaataaatg ataaattcca ataatttaag taggtatttt
     1261 ccctctattt tgttatcatt aaagataaca aaatagtaaa ttggaaagga acagttatga
     1321 agaaaaataa agttatttta agtattagtt tagcagcagc attgctgttt gctgctggtt
     1381 gttctgcaaa caacacgact tcttctagtt cgtctacaga aagttcaaaa agtacatcta
     1441 ctgcggaaag cacgactgcc agtagcgtcg atttgaacgc attggaatta ccacaattga
     1501 cgaaagatgt cgccgaaaat gaagatctgg tcgaaatggt gacgaataaa ggcacaatca
     1561 aaatcaaatt atttcccgaa cacgcaccaa aagcggttga aaactttatg acacatgcaa
     1621 aagacggtta ctacaacgga ctgactttcc atcgcgtcat caaagatttc atgatccaag
     1681 gtggcgatcc taaaggcgat ggcactggag gggaaagcat ttggggagaa ggatttgaaa
     1741 cagagatcaa caaccacctt tataatataa gaggtgcgtt atctatggct cgctcacaag
     1801 atcctaacag taacggcagc cagttcttta tcgttcaaaa tacagatgat atgcatgatg
     1861 gtcttttaaa agacgactat cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc
     1921 cttcactaga tggccattat actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg
     1981 atgctattgc aaatatcgaa acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg
     2041 ataaaatcaa tatactgcaa gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc
     2101 atagatgaca acgtatgcta ggattcaggc gtcttctttt tacataaaaa gcaagtaagc
     2161 ttccttttgt tttgctatat caggttttat agtcaagcat tttggttgac gatgaacgtg
     2221 aactcgcact cacagacttt tttatcatct acatatgctg cagataacgc tgtaccgact
     2281 gatcccttca ttttggtgat ttcaaaatga catttcagca catctcctgg aacaactttt
     2341 tgacggaatt tggccttatt gatcccaccg atataagctc tttttcctag aaactcttct
     2401 gattttagga taagtatcga acctgcttgt gccagtgttt ctagaatcaa cgcacccggc
     2461 atcactggat tgtcaggaaa gtgaccttgg aaaaaatctt cattgattgt tacatttttt
     2521 gtcgcaacga tcttttttcc tggttctaaa gtatctacat aatcaatata acaaatagga
     2581 taacgattag gaatcaattc cattacttca gtagcagata atacgtaaga cataattaaa
     2641 ctccttcttt caggcaattg ccattttcat tactttgact atcaaagtat catattccta
     2701 aatatttagc aatctttttt taatttaaga aaaaatagtt tg
//
LOCUS       DAZUMT010000185         2728 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000185 DAZUMT010000000
VERSION     DAZUMT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.193"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..335)
                     /locus_tag="ACG8FM_002873"
     CDS             complement(<1..335)
                     /locus_tag="ACG8FM_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5095456.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFM"
     gene            complement(353..673)
                     /locus_tag="ACG8FM_002874"
     CDS             complement(353..673)
                     /locus_tag="ACG8FM_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5095457.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            967..1107
                     /locus_tag="ACG8FM_002875"
     CDS             967..1107
                     /locus_tag="ACG8FM_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095458.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            1405..1662
                     /locus_tag="ACG8FM_002876"
     CDS             1405..1662
                     /locus_tag="ACG8FM_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFS5095459.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1633..1941)
                     /locus_tag="ACG8FM_002877"
     CDS             complement(1633..1941)
                     /locus_tag="ACG8FM_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095460.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            2019..>2728
                     /locus_tag="ACG8FM_002878"
     CDS             2019..>2728
                     /locus_tag="ACG8FM_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS5095461.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEG"
BASE COUNT          969 a          474 c          468 g          817 t
ORIGIN      
        1 tccataaatt tttcgaggtt aaactgagat tcatcagtca gttcgttctc aatatagata
       61 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
      121 gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca
      181 tgagataaat gaaaggcttc tgcagtttcg ccatatagat tttcattcaa aaaaatgatt
      241 ctaggcttcg gataatagaa gccaggctct ttcatttcca tgtaaactac tttcaaattg
      301 tattcgctca acatttcttt caatttcaaa tacatacaac ccatcactcc aactattcat
      361 tttcctctaa agctttagcg attgcaatcg ctttacgcat tgtctcctta gatatttctt
      421 ttccatcaaa agaaaaaaca gtatcgtttt ctgataaatc cacatgttta ggggtttctt
      481 tttcttcgcg ccccagaaga tagtctacag agacatcgaa atagtcagca atttcttgca
      541 aacgttctgt agaggggttt gagttcttaa gtctatataa gacgttttta ccatagccta
      601 ggtcttcttc aactttattt aatgattttc cctgtttttt cgctaactct tttattctct
      661 caaacgttat catatcaata ttcctttcgt tcaagaaaaa atatttaact ttatttgtta
      721 aaattcgttg actattttaa caaaaggtgt taatatcatt cttgtaaaca agtttaacaa
      781 ctaaaaagac aacaaaaaac actattgatt aataaatgcc aaccgccaag aaagctttta
      841 aaatcaatgt ttatatgtct tatttaacta tgttctgatt ttaacactgt atgttaattt
      901 agtcaacgca gttttttaaa aagttgttaa atttgtttac gaatataaaa gaaaggagag
      961 aagaatatgg aaaaaacagt ctcggcaaaa atcgaagatt tgaaattaga catactgaaa
     1021 caagcaaaag tggcgatgga acacgcggta gataaagaag actctgccat ggttgcagcc
     1081 atagcagaga ttttagctca cgtttagtca tcgtttttac ggtcttctgg aagctgacca
     1141 tagataacag cataatgaat atctaaataa gtttgaacaa tttcttttgg cgaaattgat
     1201 tcgcctttag tagatacttg tgattcgtga tttagccatg caacaactaa atccgcagcg
     1261 atttcaaacg gcgttacact ttgtgtcagt tgccataaag aatttcacaa tatgtatggt
     1321 cgtggtaata ataccaaaga gcaatttgag gaatttatgg aaattaaaaa atacaaaaaa
     1381 tcatttatta aaggaggcta aacaatgaac aaggaaatga gccaaacaat caaggtacaa
     1441 aaaatgatag atgatctaac acatggaatt gatagccaag ccgataaaat catcaaggaa
     1501 ttgcaaggac aaaaagttaa ggatgcaaaa atgcttctca agactatcaa ctttgaaatg
     1561 aatccaacaa aaagaaagct cgccgatgtg ttggaagaaa agttggcttc cgctatcaac
     1621 gagcaagaat tactatttga aaccgatact tttaacagct gatgttttat agatgacagt
     1681 ttcatccgaa tctatatcat cgccgagaga aaagaatgtt gcttttgaca aaaagccgat
     1741 aagacctaca agcggtcttg atgttgcaag agtagatacc ccaatatcac cttgcaaggt
     1801 attcacgtaa atttgagaag aatagaacat tccatctttt tcttcgatat ttgacgcatc
     1861 atcgtatcca acgaccaaag tatcggcaaa tattttgatt ttttctccat caagcatagt
     1921 aattacaaat tctggtttca taaattttca cctccttaac aattatttca gcctgtcaca
     1981 ctgataagga aattatacca aagaaaggaa tgagaaatat gaatacacca caaattttta
     2041 atttcgagca aaacgaagtt cgaactattt tagtaaatga tgaaccatat tttgtaggaa
     2101 aagatgtagc agacgttcta ggatattcga atccgcaaaa agctattcga gatcatgtgg
     2161 acttggagga taagacgcag aacgattcgt tcaccgtcaa tggaacagca gttgttttga
     2221 ttaacgagtc aggcctttac agtttaatct taaaatcaaa acttccctct gccaaaaaat
     2281 ttaaacgttg ggtaacgagt gaagtccttc caacaattag aaaaacaggt agctattcaa
     2341 acgtacctca aagttttgca caagcattgc gtttagcagc ggatttagaa gaaaagaacc
     2401 aattactcga acaacaaatt gccgagtacg aaccaaagat tagctactta gatacgattc
     2461 tttcatcgac agatacggta gctacttctc aaattgcagc tgattacgga atgtcggcaa
     2521 ttgctctaaa caaattgcta aacgagttag gtgttcagca taaagttagc ggacaatgga
     2581 tactttaccg aaaacatatg aaccaaggat acacaaaatc gcacacaagt gagataccga
     2641 aagccgatgg cggcactaaa gttgtaatga ataccaaatg gacacagaaa gggcgagtgt
     2701 ttatttacaa cttattaatc gcagaggg
//
LOCUS       DAZUMT010000186         2698 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000186 DAZUMT010000000
VERSION     DAZUMT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.194"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1064
                     /locus_tag="ACG8FM_002879"
     CDS             57..1064
                     /locus_tag="ACG8FM_002879"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFS5095462.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1076..2080
                     /gene="neuB"
                     /locus_tag="ACG8FM_002880"
     CDS             1076..2080
                     /gene="neuB"
                     /locus_tag="ACG8FM_002880"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFS5095463.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2077..>2698
                     /gene="neuC"
                     /locus_tag="ACG8FM_002881"
     CDS             2077..>2698
                     /gene="neuC"
                     /locus_tag="ACG8FM_002881"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS5095464.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQV"
BASE COUNT          999 a          341 c          532 g          826 t
ORIGIN      
        1 ttttaaagat taagaatcga attatagatg actataattc ataggaggag atataagtgt
       61 ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt gaaattgttc
      121 gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt gaagaaaaac
      181 aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta attggtgata
      241 tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt tttcatgctg
      301 ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta aaaacaaatg
      361 tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa cattttgtag
      421 gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca aagttattga
      481 gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca atattttctt
      541 gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt tttagtaaac
      601 aaataaaaaa caataaagat attactttga cagatgttga tatgacacga tttatgatgt
      661 caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa tttggagaaa
      721 catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca ttaataaaaa
      781 tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta ggtatcaggc
      841 caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc tcatatgaaa
      901 atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg tacaaagatt
      961 atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg agtggacagg
     1021 aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt ttatgatgaa
     1081 atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc tagatttagc
     1141 tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat ttcaaacatt
     1201 taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga aggataatct
     1261 taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc aatctgattt
     1321 tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta ctccttttga
     1381 tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa ttggctcagg
     1441 agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc cagttttatt
     1501 atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt tcatagatag
     1561 tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag atattaattt
     1621 attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact ccgatcattc
     1681 attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa ttgagaaaca
     1741 ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag atcctcagga
     1801 atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg atggtgttaa
     1861 gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc tagtggtcac
     1921 tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa aacgtcctgg
     1981 aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta aaagaccaat
     2041 aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact tatagtaagt
     2101 ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact acaagataat
     2161 tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg atttactaaa
     2221 gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt gtttttagag
     2281 aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt cacaaatata
     2341 tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga aatgttggca
     2401 gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg aggcgaagtg
     2461 tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc tgttcatttt
     2521 gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc ttggagagtt
     2581 tttcccgtcg gagctcctag aatagatact attttaaata atagattacc tagttttaga
     2641 gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt gcaagtat
//
LOCUS       DAZUMT010000187         2622 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000187 DAZUMT010000000
VERSION     DAZUMT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.195"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..962)
                     /locus_tag="ACG8FM_002882"
     CDS             complement(<1..962)
                     /locus_tag="ACG8FM_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5095465.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            complement(1261..1896)
                     /locus_tag="ACG8FM_002883"
     CDS             complement(1261..1896)
                     /locus_tag="ACG8FM_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095466.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..>2622)
                     /locus_tag="ACG8FM_002884"
     CDS             complement(2009..>2622)
                     /locus_tag="ACG8FM_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFS5095467.1"
                     /translation="GIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFTTVGFSLSS
                     SNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATE
                     QNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKD
                     LNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT          748 a          438 c          397 g         1039 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta
     2581 caattaacat gattaaacct ataataaata aaacaatacc ag
//
LOCUS       DAZUMT010000188         2479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000188 DAZUMT010000000
VERSION     DAZUMT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.196"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..939
                     /locus_tag="ACG8FM_002885"
     CDS             <1..939
                     /locus_tag="ACG8FM_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5095468.1"
                     /translation="INNEFNLGPNEGSSQVANNQYILLHETANETATGRNEAQYMKRS
                     WTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKATFEKNYKA
                     YVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKL
                     AYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAG
                     WHIANYKYEYIFIMDYNTGKELARVKADGIYRPDVNQAYNTLGNVGYHVSFNMRNFPN
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
     gene            1243..1650
                     /locus_tag="ACG8FM_002886"
     CDS             1243..1650
                     /locus_tag="ACG8FM_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095469.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            1664..2065
                     /locus_tag="ACG8FM_002887"
     CDS             1664..2065
                     /locus_tag="ACG8FM_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095470.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            2067..2438
                     /locus_tag="ACG8FM_002888"
     CDS             2067..2438
                     /locus_tag="ACG8FM_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095471.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          910 a          368 c          460 g          741 t
ORIGIN      
        1 atcaacaatg aatttaattt gggcccaaac gaaggtagct ctcaagtagc aaataatcag
       61 tacattttac tgcatgagac agctaacgaa acagcaacag ggcgcaatga agcgcagtat
      121 atgaaacgtt catggactag tgcttacact gcttatattg tgggagacgg cggaattgtt
      181 taccaagtcg gacaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgctaac
      241 agtcccgtgc agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac
      301 aaggcatacg ttgaattagc tagagattca gcaatgaaat atggtattcc acttacacta
      361 gacacaccgt ataatcaacc agggatcaaa tcgcatttat gggtaacaca aaatatttgg
      421 ggcgatcata cagatcctta cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta
      481 gcctatgatt tggctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca
      541 gtcattgatc caactcgagc aggtgctgca aatcctaccc tgacagatgg aacaaattac
      601 gcccacattg atcagttcgg agaaatcgaa aacgcaaact tacatgtggc tggttggcac
      661 atcgctaact ataaatacga gtatatcttc attatggatt acaatactgg gaaagaatta
      721 gctcgagtaa aagctgatgg aatatataga ccagatgtaa atcaagctta taatacttta
      781 ggaaatgttg gctatcatgt atcttttaac atgcgcaact tccctaataa gaaagtctat
      841 gtaatgatgc gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt
      901 catgataaac gctggtattt aaatattccg caacgataaa aatagcccct catttttgag
      961 gggaagtaca tatagttgtt gatatagttg ctaatattgt tagctaattt gttagtaaaa
     1021 ctgtatacga atttagatag taaaaaaaca gtttgttcct tatatagagg cacttttact
     1081 actgattaaa tgtcagatat gcttacaaat atacttataa atatgcttac aaaattagct
     1141 aatatttccg ttgacaaagc tttgttacta ttgttaaact aaattgacta gcaaaagtga
     1201 aacataaatt tattaaataa atgtaaaaat taggagaaat caatgtctta tagagtacaa
     1261 ttaataattt cagaagatgt agaaagtcag caatttggca ctaatgttat taataaagta
     1321 attaatccag ctctttctat taatgcgcct ttaattccta ctgctttatc attttctgta
     1381 acagctgtgg tgtctgagat agaggataca gaaaagataa aaatagtaga aattgaagtt
     1441 ttaaacaaga atgaaaaaca aattttttct acgggtgaag tatcggtaag cttgcctcct
     1501 caagttaacg atataaactt taatataaac gccagaaatg tcttggttga agaagcagga
     1561 gagcattatg ctgttgttaa atttaatgga actgagattg gaaggcaaat ctttgatatc
     1621 aaggtcaaca aaccagtgga aaaaaattaa ggagacgata cggatgcttg atatagtacc
     1681 atcaaataca catcggagtg gtagcttaat aaattttgct gcgatttttg cttgcgcatc
     1741 ggtaattcta gcatctccag ttgttgtgga tgcaagttct actccgaaaa ctaaaaatga
     1801 taatcaactt gtaattacta caaaaaatga gattaacaca atttcaagta atgatggcaa
     1861 aatttttgat gtgataaata cggttttaaa aagaaaagct gaaatcaata taaaatatga
     1921 tgaagattta aacttgtatt tctttgttat caaaacaaca tctgaattgt tttcctcaga
     1981 ttacgatgtt ttggatactc tcgataatgt attatctgac tataaatata tggggaaatc
     2041 tgtagtagca actttggggg aataaaatgt ttgactttaa tgaatattta tctatttcca
     2101 aacaaattga atcagatgat aaatataatt ctaaagaatc ttatagaaga actgctgttt
     2161 caagagcata ctacagtgct tttaaaaaat ctgatgagta tttgaaagaa aattatgata
     2221 ttatttataa tggaagtagc ggaaaaggaa gccaccaaac cgtttggaat ttgttttcaa
     2281 cagttaagga attgaatacc ttaggaatac ataatagtgg gtatagaatg ttagagaaaa
     2341 gaaaatgtgc tgactatatt cctaatgaaa ccattacgaa aacagatatg gcgctgatga
     2401 atcgagaagc agaaaaaata ataaacaaac tcacctaatg aatttcaagc gagtttgttt
     2461 gttatttttt tatttaatc
//
LOCUS       DAZUMT010000189         2390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000189 DAZUMT010000000
VERSION     DAZUMT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.197"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACG8FM_002889"
     CDS             197..2059
                     /locus_tag="ACG8FM_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5095472.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ACG8FM_002890"
     CDS             2059..>2390
                     /locus_tag="ACG8FM_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5095473.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZUMT010000190         2365 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000190 DAZUMT010000000
VERSION     DAZUMT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.198"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1451
                     /locus_tag="ACG8FM_002891"
     CDS             150..1451
                     /locus_tag="ACG8FM_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5095474.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1555..1755)
                     /locus_tag="ACG8FM_002892"
     CDS             complement(1555..1755)
                     /locus_tag="ACG8FM_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5095475.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            2151..2300
                     /locus_tag="ACG8FM_002893"
     CDS             2151..2300
                     /locus_tag="ACG8FM_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095476.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          768 a          454 c          408 g          735 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1381 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1441 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1501 ctatttgaca aagagccctt ttctttcgaa aagacggtta aaagtcaatt tttattaagc
     1561 tttaacaatg tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc
     1621 ttggccttct tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac
     1681 atcatttcca tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac
     1741 tgtaccgtta ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc
     1801 aataaatatt tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt
     1861 ttcaaaatag attactcatt taatatatca tgctttttct aataacacaa ggataatgca
     1921 atgaaatata aaattcattg tcttctattg tcttcgaatc gaagaaaaag ttgaataacc
     1981 gcgattgatt cactttttgt tttcttgttt atttgctttt atctgcaact tttgacttta
     2041 aaatttctta actatgaaaa cctttcaatt ggtggtatac ttataaatgg aatttcgtta
     2101 tcaacgcgtt cgttgccttt cagcaagtat ttaaagaaag gagaatggct atgacattct
     2161 ataacaaaga tatacaatat cgagtaacct tggatacaga tttgaatcta tttgtcgttt
     2221 ttgacaaaga aaataccaat catgtagcga caggtgttac gatcgaacaa gcagtgcaag
     2281 aattaaaaaa atctgcataa ggagtggaaa ccctcatatg gacgattctc gttcttctct
     2341 ttaaacgcat ctactggtag ataac
//
LOCUS       DAZUMT010000191         2340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000191 DAZUMT010000000
VERSION     DAZUMT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.199"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACG8FM_002894"
     CDS             complement(365..1657)
                     /locus_tag="ACG8FM_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5095477.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACG8FM_002895"
     CDS             complement(1931..2191)
                     /locus_tag="ACG8FM_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095478.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          781 a          441 c          355 g          763 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
//
LOCUS       DAZUMT010000192         2337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000192 DAZUMT010000000
VERSION     DAZUMT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.200"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..>2337)
                     /locus_tag="ACG8FM_002896"
     CDS             complement(226..>2337)
                     /locus_tag="ACG8FM_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS5095479.1"
                     /translation="LEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLG
                     YLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTA
                     KDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQY
                     SAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASE
                     LPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYL
                     EKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKR
                     NYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDP
                     GSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELS
                     SNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESP
                     GISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQP
                     HLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAK
                     PLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQD
                     HINLTIAKEIMDAYYDMFMAK"
BASE COUNT          597 a          522 c          425 g          793 t
ORIGIN      
        1 tgagccagaa tgtggttcat tctgactcag atttgatttt gcacaactca tcagtcctta
       61 ttgacaaaga gccacaattt ttgtcacaac cttttttgcc gagtaaacgg tgttcaaaag
      121 ccagtttctc ggtatgaagt caaatttaag caaaaatgtg agaagctatt ttcacaaaat
      181 ttttcttcat actcgaaact aagcggcttt ttcacgacct cttttttatt ttgccatgaa
      241 catatcataa tacgcgtcca ttatttcttt agcaatcgtc aagtttatgt ggtcttgttc
      301 gtctaataag ttcggcaaga caacactgat ggcaatttcc ggattgtctg ttggtccgta
      361 tgccacgata ttactattga ctgtcgcaat atctggatga ccttccgcaa ccgtttctgc
      421 agtccctgtt tttgcggaaa ggtccatttt cgcactggct aatggtttgg ctgttgtgta
      481 aggatcggta ccatggacaa cttggtggaa tccttcacgg aggatcgcca tattttctgc
      541 agaaatattg accgtatttt ctactgttgt gccaatttct ttcttcactt cacccaagtt
      601 tccattttcg tcattgccat agatcgcttt gactaggtgc ggctgtactc gtttcccgcc
      661 attagcgact gttgaagcat attgagctaa ttggattggt gtatatgtgt caaactgtcc
      721 aaatgccaaa tcggtaaagt tacctggagc ataccattct ttgccattat ccgcattgaa
      781 cttcttcata taatcaacag aacgtgagat cccaggagat tcattcggca aatcgatccc
      841 agtcgttgta ccaagaccaa actctttgaa agagtctctc aattgctgat aagctttagc
      901 ttcttcatct aaactaggta gtcccattcc aggtgtatag tctagtccta acatctttaa
      961 cgccactttg atcatatacg tattagaaga caattccgat gctttgactg catcaagagc
     1021 aacttgtccg cttcgattaa agacagagct tttttctgaa gcaccttgca gacgaatcgg
     1081 ctcatcaatc aaaacttgat ttccgctgat cgcgttactt gcccagcctg ctgtcaaagt
     1141 accagccttg actacagacc ctggatcaaa ggcgctagta atcgtgccta aagcattctc
     1201 ggtgatttct ttggaacctt gttcatgact gaaaccagtc attgctagca cttctcctgt
     1261 ctgaggattc atagccactg cataagcacc aggcgaatac tgtgctttgc cgttgttgat
     1321 caatgtttga taattccttt tcaaaatttc ttctactttt gattggaatt ctgcattcat
     1381 agataacatt aaattagaac ctttttcccc agagaagatt tctttttgat tcgaaacatt
     1441 tccttcatta tcaagggaaa cttcatattg ggttttggtt ccttgcagga catcttcata
     1501 ttgtttttcc aaataacttt gtcctacacg gtcattccga gaataacctt ttgctaggta
     1561 tttctctgct tcatctgctg gaagtccttg cttttcagtc gtcacactgc caagaatact
     1621 tttcagtgaa cttgctgcat tatattctcg tgtccagtct gtgccagtcg aaactcctgg
     1681 aagttcagaa gcgcgttctg ccacaacagc taactcttga tcagtaacac cagagttttt
     1741 gatatagaca gtgtttaatt catatgcact attcatccgt ttaaagatgg tagcaatttt
     1801 taattgctcc tcgtttaaat taatctcatc agctgtgact ttattgacca tcgcactata
     1861 ttgttcgctg ttccctaatt gcttttcttt tgccgaaagt ctttttgtgg cttctttcaa
     1921 atgtttggga tcagccagcc aaaaatcttt tttatctcta tctgtcagat tttcatctac
     1981 cggaacatcg atcagcgagt tcagcttcgt tgcgatttca agcaaatctt tagccgtaac
     2041 ttgtgttcct ctcgtatatg tgatggcctg attcgcttta ttcgtaacta aagcttttcc
     2101 tgtggagtcg tagatcattc cgcgaggagt actttcttgg accgtaatcg tagaagtaga
     2161 cttcactcgc gcctccatct cttcgccgtt cacgatctgc aaataaccca atctagcaat
     2221 caacgcaaca aataacgtaa atataatgaa aaaaagaaaa tttaagcgaa aaggaacatg
     2281 ggaccggcga aacgttttgt tcttaggttc ttgtgcacct tttcttattt tttctaa
//
LOCUS       DAZUMT010000193         2303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000193 DAZUMT010000000
VERSION     DAZUMT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.201"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACG8FM_002897"
     CDS             191..445
                     /locus_tag="ACG8FM_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5095480.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACG8FM_002898"
     CDS             446..763
                     /locus_tag="ACG8FM_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5095481.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACG8FM_002899"
     CDS             1322..2257
                     /locus_tag="ACG8FM_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095482.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          840 a          311 c          433 g          719 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag aaaaatgtct
     2281 aaacatgttg gtaaatcaac aaa
//
LOCUS       DAZUMT010000194         2226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000194 DAZUMT010000000
VERSION     DAZUMT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.202"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1038
                     /locus_tag="ACG8FM_002900"
     CDS             <1..1038
                     /locus_tag="ACG8FM_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5095483.1"
                     /translation="FRGMLIKNLVDQGNEVIVSLPKGKNIPKLIELGCQIIETPIDRR
                     GINPKTDFKLFFNYCNILKKIKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTA
                     FQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVE
                     HFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGI
                     KIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDI
                     PGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKK
                     VVDETIKSLFS"
     gene            1045..1440
                     /locus_tag="ACG8FM_002901"
     CDS             1045..1440
                     /locus_tag="ACG8FM_002901"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5095484.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            1437..>2226
                     /locus_tag="ACG8FM_002902"
     CDS             1437..>2226
                     /locus_tag="ACG8FM_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5095485.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHN"
BASE COUNT          873 a          251 c          430 g          672 t
ORIGIN      
        1 tttcgaggaa tgctcataaa aaacttagta gatcaaggta atgaagttat tgtatctctt
       61 ccaaagggca agaatattcc taaattaatt gaacttggat gtcaaattat tgaaactcca
      121 atagatagaa gaggaattaa tccaaaaact gactttaaac tattttttaa ctattgtaat
      181 attttgaaaa aaataaagcc agatatggtc attgcttata ctatcaaacc aaatatatac
      241 ggtggattgg catgtcggtt aagaaaggtt tcttatgcat taaatataac cggattagga
      301 acggcatttc aaaaaaaggg ggttttgaaa agtcttattg tatttttgta tagattagca
      361 gctaaaaaat ctaaggtcat atttttcgaa aattttgaaa atatgaaaac atttcagact
      421 ttacaaattg ctaaacaaag taaatacaag ctattaaacg gtgcaggagt agatgtagaa
      481 cactttagtt atttagaata tcctaaagat gagcaagttc attttttatt tattggaaga
      541 attatgaaag agaaaggtgt agatgaactt tttgaagcaa tgaaaaactt atatagtgaa
      601 gagaaaaatt gtgtacttga tgtccttggg tattttgaag aagattacgg cataaaaatt
      661 gaaaaatacg aaaaagaggg atggttaaaa tatcatggat atcaatctga tattcgtccc
      721 tttgtagaaa gagcacattg ctttgttttg cctagttggc acgaaggaat ggcaaatact
      781 aaccttgaat cagcagcatc agggagacca attattacaa gtgatatccc agggtgtaaa
      841 gaagcagtta ttgaatcgaa gtctggttat ttatgtgaaa gaaaaaatag ttgtagtctc
      901 tataattcga tgaataaatt tctaaaatta tcatatgagc agcgcattaa catgggaaaa
      961 atggggagaa attatatgat taaaaaattt aataagaaga aagttgtcga tgaaacaatt
     1021 aaatcattat ttagttaaaa agatatgttt tgcaaagtat gtgatagaaa agagggattc
     1081 tgtggcttca gaatagatat caaagaattg gcaaaagttt ctgatatttt tttatttact
     1141 acattacaag aaggaatgcc aagatctatg atggaagcta tggcaagtgg attgccatgt
     1201 atagcaagta aaatccgagg taatgtagac cttttagtgg aaggaaaagg tggatatctt
     1261 gttccagcta aagatgcaga attagtagca gagtgtttga gagaactaat caataataaa
     1321 tctcttagaa agtatatggg aaatgagaac ctttccagaa ttaaagaatt cgaggttagt
     1381 atcgtaaaaa aaagaatcaa agaaatatat gaagaagtta taggtgagag tgtaaaatga
     1441 aaaatccaat gttttcaata atagtaccta tctattgcgt tgaaaaatat ttgaaagatt
     1501 gcttagattc aataagaaat cagacattta aaaattttga agcaatacta gttgatgacg
     1561 gtagtcctga cgaatgccca gaaatatgtg actattattc ccaaatagat ggacgtttaa
     1621 aagtaattca taagaaaaac ggtggaataa tagatgcacg gaaaacagga gtcactagtg
     1681 ctatgggaga gtatatagtt tttctagatg gtgatgattg gcttgcaaat acttatttag
     1741 aaaaatatgc ggaagtaatt actaagtaca atccagatat aatatgtagt gggtattttg
     1801 catcacgttc caacaaaatt agaaaaagag aattgtcttg ctcgtttgga tattatgaca
     1861 aagataaaat tataaatttg atttatccta aattaattga ggatgaacat ggagagtatt
     1921 ttagaccaca attatgggca aaagctttta gaactactct gtacaaggaa caacaacaag
     1981 tatctgggca agttagtgtt ggtgaagatc atgcatgtac aaaaccatgt atttaccatg
     2041 caaattcgat atatatgatg gaaaaatgtt tatattatta cagaataaac gaaaattcta
     2101 taacaaaaaa accacaacca tattattggg aaggaccaaa ggctattgga gagcattttg
     2161 aaaagcagat tgatatgaat aagtatgatt ttcaagatca agtatataga aatgttacac
     2221 acaatt
//
LOCUS       DAZUMT010000195         2163 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000195 DAZUMT010000000
VERSION     DAZUMT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.203"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACG8FM_002903"
     CDS             complement(45..1364)
                     /locus_tag="ACG8FM_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5095486.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACG8FM_002904"
     CDS             complement(1361..2014)
                     /locus_tag="ACG8FM_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5095487.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          718 a          330 c          175 g          940 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 agg
//
LOCUS       DAZUMT010000196         2157 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000196 DAZUMT010000000
VERSION     DAZUMT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.204"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..521
                     /locus_tag="ACG8FM_002905"
     CDS             <1..521
                     /locus_tag="ACG8FM_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095488.1"
                     /translation="EYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMA
                     GCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRC
                     RRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFARE
                     DEDNPKTYDILA"
     gene            complement(582..947)
                     /locus_tag="ACG8FM_002906"
     CDS             complement(582..947)
                     /locus_tag="ACG8FM_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS5095489.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1023..1268)
                     /locus_tag="ACG8FM_002907"
     CDS             complement(1023..1268)
                     /locus_tag="ACG8FM_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5095490.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1338..1592)
                     /locus_tag="ACG8FM_002908"
     CDS             complement(1338..1592)
                     /locus_tag="ACG8FM_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095491.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1756..2079)
                     /locus_tag="ACG8FM_002909"
     CDS             complement(1756..2079)
                     /locus_tag="ACG8FM_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095492.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          814 a          364 c          376 g          603 t
ORIGIN      
        1 aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc gttattattt
       61 tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta tcaatattaa
      121 cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc acaaaatcaa
      181 ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac agaggcttaa
      241 atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt agtaatcgaa
      301 gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc aatctgtgct
      361 gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg agatgttcat
      421 ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt tttgcacgtg
      481 aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg gttaaagctt
      541 tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag ataaattcct
      601 ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc cggataaatc
      661 ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt gccacgatac
      721 ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc atccatgaca
      781 agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc cacagtagca
      841 tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg tcctaaaagc
      901 tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga aatgtctcct
      961 ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa ggctggaaaa
     1021 aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac ggaaatatcg
     1081 taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc ttcttcgtac
     1141 ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg agataaagat
     1201 cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat agcttcttcg
     1261 atactcattt aatcaccttt cattatagta gaatagttat aatgactata gaaagctaca
     1321 catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag aatgacgatc
     1381 aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg ttcaaagtag
     1441 ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat agctaaataa
     1501 aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc gaccgcaggc
     1561 acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg attcatataa
     1621 taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat aaaagaagaa
     1681 tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg aactgttccg
     1741 ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt aattcaatac
     1801 atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc catagcgaat
     1861 ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc ctttatagct
     1921 gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc caataaacag
     1981 aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa ctaaatcgaa
     2041 taaaatcgca ataaatggca aaataatcac tcctaacatt gagaataaat aaaagaataa
     2101 cattttttct aaaataagca aataaaaatt aaagtaacaa aatagttatg acacaag
//
LOCUS       DAZUMT010000197         2048 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000197 DAZUMT010000000
VERSION     DAZUMT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.205"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACG8FM_002910"
     CDS             complement(<1..426)
                     /locus_tag="ACG8FM_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS5095493.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACG8FM_002911"
     CDS             complement(535..1566)
                     /locus_tag="ACG8FM_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5095494.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          630 a          331 c          354 g          733 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgcttatt
     2041 gatggatt
//
LOCUS       DAZUMT010000198         1986 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000198 DAZUMT010000000
VERSION     DAZUMT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.206"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACG8FM_002912"
     CDS             <1..152
                     /locus_tag="ACG8FM_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5095495.1"
                     /translation="YRKLDGNLEHQDQVTLFLDFPFLYPGKNEIKWTKNIHSFEIMPR
                     WWRKV"
     gene            149..>1986
                     /locus_tag="ACG8FM_002913"
     CDS             149..>1986
                     /locus_tag="ACG8FM_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5095496.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          738 a          244 c          394 g          610 t
ORIGIN      
        1 cttatcgaaa attagatggt aatttagaac atcaggatca ggtcacctta tttttagatt
       61 ttccattttt atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt cattcatttg
      121 aaataatgcc taggtggtgg agaaaagtat gaaaccaaga atatatagtc ctactgaaac
      181 agatttctca acgaatggct taggaatttt aaaggattgt acaagatgtg gaatatatga
      241 agtagctaat ggaaaatatg aattagagtt ggattatcct ttaggaacta gatttgatga
      301 atattttgaa aatgactatc aaataaaagc aaagccaaac gatcaagaag agtatcatat
      361 cttttttatt gatgataaag atatagatac ttttttaaat acagtaacta tttatgctca
      421 gagtcgtaca aatcgacttg gaagacgggt agtcactctt gtggaagtgg actctaaaac
      481 tggtcaagaa gcgatatcaa ttattgaaac taaaatggat aaaaaatctg acatacgact
      541 ttattctgat attacggctg tttctagcac aatctttgaa gcgagaaatg ttttaaattg
      601 tattgctggt gaacaaggat cattgcttca atattggggc ggagaaatta aacgtgaacc
      661 atttaagctt tctttgttaa agcgaagagg tcgcaataat gttggaacga ttcggtatgg
      721 aaaagatatg tctggtttga aggtcaaatt agattggaca ggtataaaaa caagaattat
      781 tccatatgct gatcctcaaa gtgaagtagg tacgactaat cgaatttatg gttcgccagt
      841 agatagtgcc tatattaata actatcctga tgtctataca gaacatgttc agtttacaga
      901 agaacaagga gtaaaagaca ttaagagctt aaataaaata gctaaaaatt actttaaaac
      961 gatcaatcct ggctgtgata agcctaaagt ttctattacg gttgaatttg ataagttgac
     1021 tgatagtgaa gaagcgaaag aatttgcgaa gattagaaat tatggtctgt ttgatacatt
     1081 taaaatatat cataaaaaat ataatattta tcttgaatca aaggttagtg gactacaata
     1141 cgattcttta tctgaaaaaa ctttgaaatt agaagcagga gacattcaag ttgcttttta
     1201 tcagcaacaa gctgtaacta ttcaggataa attaaaagat tatgcaacga ataattatat
     1261 gagtgatttt aatgattatg tttcttcaat gattacaggt caaggcagtg caggaggata
     1321 tgtagtttta tggccaaaag aaaaaccttc caacatcttt attatggata atccagattt
     1381 agaaaaggca agagaagtac taagaatgaa taaaaatggg attgcttttt ctaagaaagg
     1441 atggcggggg ccttttaatt cagcttggac attagatagt atatttaatg ccaattttat
     1501 acaaactgga ttaatcaaag cagatatctt tcaaaattct tttaacaaga ctggagatgt
     1561 attgaaatta gttaatggac tactccaaat ttggaacaac aaaaagaaaa taatggagtt
     1621 aactaaaaaa ggaatggaat tttggaattc taagggttca attggaacga ttggaacaac
     1681 tgattctgtt ggtaatcctt ttcctggggc ttctactcca acccctattg aagataattc
     1741 tttagttatt cgtacaaatg gagacggcaa atatattttg atttctccta aagtaggaaa
     1801 aggattagtt ttattaggaa atggtaaagc aatttatttt ggagacttag atgtacaagg
     1861 aaaacttaca gttaaaggaa aagaaataat tcctggacaa ggtggtggtc catcgggagg
     1921 tggagaatct actggtgggt atcctagtga attaaaaaca gatgcagaaa agagagcttg
     1981 gagaat
//
LOCUS       DAZUMT010000199         1979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000199 DAZUMT010000000
VERSION     DAZUMT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.207"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..367
                     /locus_tag="ACG8FM_002914"
     CDS             <1..367
                     /locus_tag="ACG8FM_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS5095497.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..749
                     /locus_tag="ACG8FM_002915"
                     /pseudo
     CDS             567..749
                     /locus_tag="ACG8FM_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1330..>1979
                     /locus_tag="ACG8FM_002916"
     CDS             1330..>1979
                     /locus_tag="ACG8FM_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5095498.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          657 a          305 c          461 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcgttaaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacga tcgtgttaga ttgtgaaaac cgatagctga atgaaataaa cagcgggtgc
     1021 gccctttcca tttcggcagg aggaggctca aggggttcgg ggaatgaaat tccctgaggt
     1081 ctttttattt ttttaggagg agtgctataa tcaaagaaaa cgagaaaaaa gagcaaaata
     1141 aaagccgagc agtgcgctaa cactctcgac tatttacgga aactacttca cctagctccg
     1201 taactcaggt tcattatacg aatattttta ggtttcttca agctagagat ttttggatat
     1261 gaatttttag ggttacgtga actctggaaa ttctttccaa aagtctcttt tttcgtctgg
     1321 aggacgaaaa tggcaaatga aaatcagttg agtgaaattc aatcaaaaat tgaaggtcga
     1381 ggggcagaaa aaagaacaac gataaatcag ctcgaaaaac ttaaccgtat ttctaacagg
     1441 cggttaagtg ttgacgataa ggtacacccc ctaaaattaa gttggtcaat cgatcgaatt
     1501 acaatcgtcg gaaaactgaa tgataccata gcttattgga cgcaaaatgg agttgtgttt
     1561 gtcgattttg agcaattaat gcgtttgaat gaaaatcaag ggtatttgaa gtcggttggt
     1621 acaaatggtt ggcaattatt tgaccaatat ggagaagata tcgcctatat cgaaatgtta
     1681 aaatttcacc ctgacaaagg tcgtattgat tttaatccaa ataaaattgg cgagttttta
     1741 aaggggtcga tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca
     1801 gatattgctt gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt
     1861 gaccctgtgt cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg
     1921 ggtagtcgtg ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacg
//
LOCUS       DAZUMT010000200         1918 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000200 DAZUMT010000000
VERSION     DAZUMT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.208"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG8FM_002917"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG8FM_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG8FM_002918"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG8FM_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          672 a          266 c          251 g          729 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaa
//
LOCUS       DAZUMT010000201         1874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000201 DAZUMT010000000
VERSION     DAZUMT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.209"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            239..436
                     /locus_tag="ACG8FM_002919"
     CDS             239..436
                     /locus_tag="ACG8FM_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095499.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            542..1024
                     /locus_tag="ACG8FM_002920"
     CDS             542..1024
                     /locus_tag="ACG8FM_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095500.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1082..1678)
                     /locus_tag="ACG8FM_002921"
     CDS             complement(1082..1678)
                     /locus_tag="ACG8FM_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5095501.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          587 a          274 c          373 g          640 t
ORIGIN      
        1 aaatggaagt attatatttt aatatgtagg aaataattag tttttaagga atatgtttta
       61 attttttgaa aaaaattctt ctaaatacaa cagctgtttt aattgtgaaa acgaaaacag
      121 tacgctataa tgcctgtaag gttgattttt aactgtaaat aaaaatcaat gagttaaatc
      181 tccaaagaaa tagcagtggt aatgtggaga aaagttccag cagtttagga ggattttgat
      241 gacggaagaa atgattaatt taggagaaca atatgcatgc aaaccaattg gttttacaaa
      301 aacggtcatt ggtgaagttg tatcaaaaat gacaaactgc gccgtggtca aagttgccca
      361 atgtgctgca gaggaccaag aattattaga tgaaaaagca agcatggttg ttgcaaaata
      421 tgatacattt gaataaagaa aacaattgga cgtgcggcgt gctgcaagtc caatttcttt
      481 gagaaggtaa atcagtattc ttttcctatt ttctggagaa gaaaaagcga ggcgagttgt
      541 catggtaaca ttctttacgg ttctattgtt tttattcgtt gttgtgatgt ttttatttct
      601 ttggctggtt cgaaaagaaa ttatttatcg gactgtgcga aatcgatggg tctacttggt
      661 gatacccttc ttagtgtcac tagtcatttg gtatacactc atcagtcatc ctacagcaga
      721 tgaattggca aaagggatct tgtcagcaat gatttttatt agttttttgt tggacagtag
      781 aggaatcaca gaagaaggac ttgttttgaa tagctttgac aaaaaaggtg ttcctttttc
      841 tgagatcaat aaaattgtat tatatcagcc aaaaggttct aaaatcgtaa aaatgaattt
      901 tttccgaaac ggatggcgag gaccgatgct gaaattttct gcatcattag aagaattagt
      961 tccatttttg tcccaacgct taaacgatga ggcagagatt gatattatga ttgatccaga
     1021 ataaaaacag aggttgcgac aagatgtttg tcacaacctc tgttttttat ttcaaataaa
     1081 attatccaaa tacgcggaag cgatcggcat cgttcatgta ttctttttcc tcagccggtg
     1141 tttctggtga accgaatact aattgcgaac gtagtttcca gtttgaaggg atattccatt
     1201 cttttgcaac tgcttcgtca ataactgggt tgtaatgttg caagtttgcg cccaatcctt
     1261 tatcaactaa tgctacccaa gtattagctg ttgcgattcc gtttgattgt tctgaccaat
     1321 ctgggaagtt gtctgcatat aattcaaatt gttcttgtag accttttacc acgtcagtat
     1381 ctttgaagaa taatactgta ccataaccat ttttgaaacc agctaatttg ttttgtgtat
     1441 ttgggaaagc ttctgctgga gtcaatgggc gaagtgcttc ttcagtgatt tcccaaagtt
     1501 tttcatgtgc atctccgaaa aggataactg cgcgtgttga ttgagcattg aaagcagttg
     1561 ggctttcttt aattgcttct ttgatcaaag ttgttaattc ttcattagaa agtgaaacat
     1621 tacgtcctag atgatagatt gaacggcggt ttttcaaagt gtcagtaaaa gttgtcataa
     1681 taaaatttct cctttgattt gcgttttgca tttttttaat tgattacgaa atgaagtata
     1741 gcaccttaca aaaagtaagt aaacagatat gctcacaaaa aataagcgaa gttcaaaaaa
     1801 caattttaat aagaaaaatc gttatgtaag gattttctta gttatcgctt tcattgttgt
     1861 gttttaggaa tttt
//
LOCUS       DAZUMT010000202         1854 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000202 DAZUMT010000000
VERSION     DAZUMT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.210"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8FM_002922"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8FM_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS5095502.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACG8FM_002923"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACG8FM_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS5095503.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          551 a          329 c          299 g          675 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcga
//
LOCUS       DAZUMT010000203         1847 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000203 DAZUMT010000000
VERSION     DAZUMT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.211"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="ACG8FM_002924"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="ACG8FM_002924"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5095504.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DAZUMT010000204         1793 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000204 DAZUMT010000000
VERSION     DAZUMT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.212"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8FM_002925"
     CDS             131..505
                     /locus_tag="ACG8FM_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5095505.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8FM_002926"
     CDS             complement(619..1248)
                     /locus_tag="ACG8FM_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5095506.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8FM_002927"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8FM_002927"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG8FM_002928"
     tRNA            1668..1741
                     /locus_tag="ACG8FM_002928"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
BASE COUNT          621 a          263 c          313 g          596 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt ttt
//
LOCUS       DAZUMT010000205         1720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000205 DAZUMT010000000
VERSION     DAZUMT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.213"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1551
                     /locus_tag="ACG8FM_002929"
     CDS             <1..1551
                     /locus_tag="ACG8FM_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFS5095507.1"
                     /translation="MESVKKESYLDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGR
                     PTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAIT
                     YSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQF
                     GKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTF
                     RKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHT
                     PLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGS
                     LMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAK
                     LNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKE
                     LHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDEL
                     IPLENELR"
BASE COUNT          620 a          275 c          349 g          476 t
ORIGIN      
        1 atggagtctg tcaaaaaaga aagttatttg gaccctactg tttttgaagc agaggaacat
       61 atccaaaaag tcattcataa aatgttccgt ccagacaatg cctatacaaa atttgatttt
      121 cgaaatggcc gtccaacgaa gtctgcgatc ttaaccacga gttccattga tatggccaaa
      181 aagtattatc gggccatcaa agagatgacg aaagaatctg attggttaat aaaagagttt
      241 cccaatcagc ctatccgtga aggacgaacg atggaggatc ctgatttccc acgaatcgct
      301 attacctatt cattagaaga aaatagccga gattcaagtg cccaacaaga agaaatgcaa
      361 aaaattattg aggaatataa tcagtattat gatactgcct ggtcactagc agacattgag
      421 cgttataacg gagacatcaa taatcgtttg gcacgaaaaa gagcggaatt caaacaattt
      481 gggaaacaaa tcgatttagt aatcgtagtg gatcgtctat tgacaggttt tgacgcccct
      541 acgattcaaa ccctatttgt ggatcgtaat ctagagtatg caggattgat ccaagccttt
      601 tcacgtacga accgtacgta tcctgaaaaa acaaaaggac taattgttac tttccgaaag
      661 ccagcgacaa tggagaagaa tgtcgaggat gcgacaaaac tgtattcaga agctaaagaa
      721 gaatcgggtt tgatttatcc aagttaccaa gaatccaaaa aaagattcaa gcaagcatat
      781 ggtaagttaa aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc
      841 cgtatcgagt acgtcaaagc atttcaagaa ttgaatcgtg cgtatgaatc gttagtgact
      901 tatgatgatt acaatgatga catggtgcaa tcttcaactt tgaacaatca aattcaactt
      961 ttggaagaac aaattggcgt gtacgagacc gtgaagggtt cattgatgga ggaagaacca
     1021 gaaaaagagg gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg
     1081 tatgatattg attctactta tattgatcaa ttactaggaa cttatgctgc gaatagttca
     1141 gatgcacgag aagaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa
     1201 gaagtttacc gacaaatttt aaatgcattt gatgtgggga ctttagatag taatgaagat
     1261 atctttgtca ttaaacgtcg ctattttaca caagcaagtg ataatttgat tcatgccttt
     1321 tccaatgagt ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatatgcca
     1381 ggtgaagatc caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat
     1441 aaagccaaac atccagaagc gaaaccattt aaatacccac aagaaatgaa gcgagcatgg
     1501 cgtaaaatgt tagatgatga gttgattcca cttgaaaatg aattgaggta aacgagaaga
     1561 gttaagaaaa ttgtacgatt caaagatagt agtaaagtgg aagtgtagaa aatagggata
     1621 gatgtctttt tacttgttca cattttataa cggagtattt aagaaagcta ttgaataatg
     1681 attcttccat ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DAZUMT010000206         1680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000206 DAZUMT010000000
VERSION     DAZUMT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.214"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG8FM_002930"
     CDS             64..972
                     /locus_tag="ACG8FM_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFS5095508.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACG8FM_002931"
     CDS             972..1592
                     /locus_tag="ACG8FM_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095509.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          602 a          246 c          377 g          455 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
//
LOCUS       DAZUMT010000207         1677 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000207 DAZUMT010000000
VERSION     DAZUMT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.215"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG8FM_002932"
     CDS             64..972
                     /locus_tag="ACG8FM_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFS5095510.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1589
                     /locus_tag="ACG8FM_002933"
     CDS             972..1589
                     /locus_tag="ACG8FM_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095511.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          620 a          226 c          348 g          483 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc
     1501 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     1561 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
     1621 gcaatgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaa
//
LOCUS       DAZUMT010000208         1642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000208 DAZUMT010000000
VERSION     DAZUMT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.216"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..240
                     /locus_tag="ACG8FM_002934"
     CDS             <1..240
                     /locus_tag="ACG8FM_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095512.1"
                     /translation="KKLLKQFAGIGNANVKFICLSCGEKESIPMDVVKFLDDADIAND
                     PNNPPQFTCEKCGKEMYPEYYRNALGIEFKISDVQ"
     gene            394..>1642
                     /locus_tag="ACG8FM_002935"
     CDS             394..>1642
                     /locus_tag="ACG8FM_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095513.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEK"
BASE COUNT          590 a          250 c          367 g          435 t
ORIGIN      
        1 aaaaaacttt taaagcaatt cgctgggatt gggaacgcca acgtgaaatt tatatgtcta
       61 agttgtggtg aaaaagaatc aatcccaatg gatgtagtaa aatttttaga tgatgcagat
      121 attgcaaatg atccgaacaa tcctccccaa tttacctgtg aaaaatgtgg gaaggagatg
      181 tatccagaat attatagaaa cgcccttgga atagagttta agatcagtga tgttcaataa
      241 gaaggttaat catacaaaaa gtccaccgga gaattcattc tccggtggtt ttcttaaaca
      301 gtaaagaaag tataaaaagt taagggctaa aaaggctgca ctaccagtct tttagccctt
      361 ttcattttgt ttttgaacaa gtaaaatatt actatgaaag cgaatattct cgaagcaatc
      421 ttttttgacg aacaccataa ttgggataac tttgttgaga aatacgggcg aaagattcgg
      481 ctagtcgtca aacgggaagt aaacaagttt agatattgtg gagatattca aaaaggatac
      541 cgactgtttg tctgcgaggg atgtcatcaa acaaagtttg ttcccttgaa atgtcgtgga
      601 aagttctgtc caacgtgtgc aacaggtgaa agccaaaggt ggtcagcggt tgtggcgaat
      661 gatatgttta aagtgacaca tcggcacgta atctttacga tcgatgaggg gttacgggaa
      721 atctttatga tggaaaacta tcgagattcc ttactgaaag gactaatgga tgaagccgca
      781 aaaattattc ttgcctattt tgagaaaaga aaggtgaagg caggtatagt agctacaatc
      841 cacacctttg gatcaaaact agaatttaat ccgcatgttc atatggttgt tactatgggt
      901 ggcttaacaa gagatggaag ttgggaagaa tacgattatt tacctttttc tatgttaaga
      961 aaatactgga aaaatgctgt attgaaacta attagacgaa cgttaggtga gtgggacaag
     1021 caaagagttc aatcaagatt acaagctgcg tacaagaaca atggagaagg gttttacgta
     1081 aatgctccta aaaggagcag gacaaatcta aagtcactac tccaatacat cagtcgttat
     1141 atgaagcgtg gcccgatcgc attaagccga atcttaatgt atgatggcga cactgtcatg
     1201 ttcaactaca tggacaaacg aacgaatact aaagagacga tgaccatgag tatagacgaa
     1261 ttcatagctg cactgattcg ccatattcaa aataagaatt tcaaaacgat tcgtcgctat
     1321 ggaatctata gtcgtagaat caaaacatta atgaaaaagg tcatgaagga gtatcagaaa
     1381 cgagtaaaaa agctgtttat caatctgaat gaggccttaa gaccaaaatc ctgggaagaa
     1441 agaataaccg aagaatttgg tgaaaatccc ttagaatgtt cttgttgcgg tgagtactat
     1501 gaattcgttg ggatgagtgt gcgcaaaaat ggacggttag ttattaaata tgcgaaagat
     1561 aaccatgcaa agcggtttat gcgagaggag aatcgaaaaa ttgaccaaaa agaattcagg
     1621 caacagtatg aagaagaaaa ag
//
LOCUS       DAZUMT010000209         1640 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000209 DAZUMT010000000
VERSION     DAZUMT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.217"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..>1640
                     /gene="ltrA"
                     /locus_tag="ACG8FM_002936"
     CDS             416..>1640
                     /gene="ltrA"
                     /locus_tag="ACG8FM_002936"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5095514.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRL"
BASE COUNT          586 a          299 c          388 g          367 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag
//
LOCUS       DAZUMT010000210         1620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000210 DAZUMT010000000
VERSION     DAZUMT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.218"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..475
                     /locus_tag="ACG8FM_002937"
     CDS             <1..475
                     /locus_tag="ACG8FM_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095515.1"
                     /translation="FVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLH
                     PRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIML
                     AKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            462..>1620
                     /locus_tag="ACG8FM_002938"
     CDS             462..>1620
                     /locus_tag="ACG8FM_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5095516.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGII"
BASE COUNT          521 a          210 c          261 g          628 t
ORIGIN      
        1 ttttgtcgga gataaatgta tcttttttga tcaaccttta ttatctgatg gttatggcat
       61 agatgaaaca gatattttcg ctaaagtaaa tagtatattt tctggaaaaa aagaaacttt
      121 aattaagctt catcctagag caaggacaaa tcgttatggt gaaaaaaaca tattgaattc
      181 cagtttaccg tttgaagtct tgctacttga aaatgacttc tcttcaacac ttttgattag
      241 tcctatatct acaatttctt tttcaccatc tatgatgttt gaaaagacga tcaactctat
      301 tatgttggct aaattaattt taaatgaata tacagcatta tctgaagata aaaaagaaac
      361 attgacaaat attgttcttt tctgtgaaca ttataaccgt ctatcagaag aaaaaatttt
      421 actcccagaa aactgggcag aattaagaaa gttggtagag tatgggatta tttaacacac
      481 tttccaagtc aaaattagtt aagtccagtt tctggtatac tatagggaat ttttttatta
      541 agggaatttc attcataact attcctattt ttgtaaatat tactactcaa gcagaatatg
      601 gtttgattaa taatttcact tcatttgtat caattttttc gattcttgtt gggctaagcc
      661 taaattcggc aattaataat gcaagttttg actataaaga tgacataaat gaatttatgt
      721 catcggtact ttctctagct acactttcgt taattgtgtt cttattaata gggaacatat
      781 attttttgtt ttcaaatgct ttttttgata tgaaccaact tgtatttaat ttgatgttat
      841 tacaaagttt tgctagtttt gtaattagtt atatccagtc ttattttacg attaacttta
      901 accattttaa atatttgatt ttatctttta tatctacttt tttgaatatt gcaatctctg
      961 tagctttgat gcagacatta tattttgatg ataaatatgt tggcagagta gtcggcggaa
     1021 cactgtcatt tgtgttgata ggtttagtaa tttatttaag aataatgata aagggtgctc
     1081 atttttttga taaaaaacag tggagatacg ctttaaaaat ctctttgccg ctaatcccac
     1141 attccttagc aaatatagtt ttagcacaat tcgatagaat tatgattaat tcttatttag
     1201 gtcctgctgc ggggggaata tattcctaca ttagtaacat aggtattatt ctaagcgtaa
     1261 tatggatgtc tactaataac gcctgggttc catggttcta tggtgagatg agtaaaaata
     1321 atgaaaaaaa agtgaagaaa gttagtaact attatttagt agtctttact ttgattacga
     1381 tgattgtaat ggtagtttcg attgatttag caaaatttat ggctccgaaa gactattatt
     1441 ctgggttatc tttagtggtt ccgataacct taggctattt ctttcagttt ttatatagtt
     1501 taccagttaa tgctgaattt tatgagaaaa aaacatcgtt tattgcgatt ggtacagtag
     1561 caagtgctat agttaatata ctattaaaca tgattttcat acctaggtta ggaataattg
//
LOCUS       DAZUMT010000211         1617 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000211 DAZUMT010000000
VERSION     DAZUMT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.219"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..1570
                     /locus_tag="ACG8FM_002939"
     CDS             104..1570
                     /locus_tag="ACG8FM_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS5095517.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          452 a          292 c          335 g          538 t
ORIGIN      
        1 ttatagaaaa ttagtttata tttcttaaat aagtttttta gttaaatttt tcggtaaaga
       61 tgtataatat tttctgaaaa tttaaagaat tggggtatag attatggaaa aacagcaaga
      121 caagggcttc ttcgggcagc cgaagggatt gtcaactttg ttcttcactg agatgtggga
      181 acgatttagt tattacggta tgcgtgcatt acttatctat tatatgtacg atacagttgc
      241 caacggggga cttggattac cacgagcaac agcattagct atcatgtcga tctacggatc
      301 tttgatctac atgtctagta tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa
      361 acgcactgta ttcttcggtg gagttttcat tatgcttggc cacatcgtct tggctttacc
      421 atttggtgta agtacattgt ttgtttcgat ggcattgatt attatcggaa ccgccttttt
      481 gaaaccaaat gtttctggca tggtcggaca tctttattca aagactgact tgcgaagaga
      541 tgcaggattt tcgatttttt acatgggtgt caacttaggg gcattattag caccactaat
      601 cgttggtaca atcggtcaag aatataacta tcatttaggg ttctctttag ctgctattgg
      661 gatgttcttt ggattattac aatacatcat ccaaggacgc aagacattag acggagtggg
      721 tacaacaccg ccgaatcctt tgacaactga agaacgtaaa ggattcatcc gtaacttgat
      781 catcgcactt gttgttatcg ctgcagtatt cggcggggca caactaactg gtcatctgac
      841 aattgacttt tttgtcaata caatcagtgt attggggatt atcttaccgt tgtattactt
      901 tatcaaaatg ctgacttcaa aagatgtaac tgctgaagaa aaaccaagag tatgggctta
      961 tatcccatta ttccttgcag caatcatttt ctggtcatta caagaacaag gttcatctat
     1021 cttagcttta tttgctagtg aacgtacaca atctacgcta tttggtgtaa cgatccaagc
     1081 aagctggtat caatcattga atccaatttt catctttatt ctgacaccag tattcgtaac
     1141 gatgtggacg aaacttggtg aacgtcaacc atctacagta gtgaaattct ctcttggact
     1201 tttatttgca ggattatcgt acttgctatt gatgttgccg ggtatgcttt atggtacaag
     1261 tggaaaagta agtccattgt ggttgttggg aagtttcttc atcgtggtta ttgctgaatt
     1321 gtgtctttca ccagttggtt tgtctgtgac aacaaaatta gcaccacgag catttgaatc
     1381 acaaacaatc gctatttggt tcttagctga tgcttcttca caagcaatca atgcacaaat
     1441 tgcacgcttc tacacaccgg gaacagaatc tgcttacttc ggtattatcg gtgcagtagc
     1501 aatcgtcggt ggtatcttgc tatttactgt gaaagaacca atcaaaaaac taatgggctc
     1561 tattcattaa tcaatgaaca taaaaacagg aattgcttat ttgcagcagt tcctgtt
//
LOCUS       DAZUMT010000212         1558 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000212 DAZUMT010000000
VERSION     DAZUMT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.221"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..841)
                     /locus_tag="ACG8FM_002940"
     CDS             complement(<1..841)
                     /locus_tag="ACG8FM_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS5095518.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRI"
BASE COUNT          500 a          312 c          266 g          480 t
ORIGIN      
        1 ctattcttga gctggctgca gctagtgagt gtctttcatt tatccataag ataaagcttg
       61 gattcaataa ataaataaat gttttggtta aagctgtgac cagcaaatga ggaaccgtgc
      121 gagccagttg atctgccaat gcaaacattt ctttaggtga aggcgctggc gtgtctgcca
      181 acggatactc cagtcccaat gcttggtaac cgtgcatacc atagcagtgg tacggtagta
      241 attctaacgg tacgaaccga tcaagatctg ccacaaaaca agcaatcgtc agcagctcgg
      301 tcaggttcat attgatacct ggaaccacag gaactcgaat tcgcacttcg cagttaggat
      361 actcaattaa aaagcgacga atattcttca aaatttgttg gttatctaag ccggtccatt
      421 gtttgtggag cttgctggta aacattttta catccacaaa caatgtatcc acatagggag
      481 ctaccttttg gatttcatta taagcaccac aaaagctggt ttccagtacg gtattgattc
      541 caatgtattt cgatttttgc aaaatttctt tagcaaaatc cgcctgaacc agtgcctcgc
      601 ctccgctaat ggtcactccg ccatccgagt gaaagtaaaa aacagcatcc ttctcaatct
      661 catccattac ctcttcagcg gtcatggtct ggccaaatcc tttttcaatc tgcttgcttt
      721 gtgattcggg aatcgaacac caggcacagc gtaatgggca gccctttaaa taaacaacgg
      781 tccgtaaacc agcaccatca tgtagagaac cctgttctat tcttaatact gttccttcca
      841 tatagatttt tgaattacac actagtttaa actaaaacac acaaggaaaa acttaccatt
      901 taatcataaa attagctcat ataaatatac taggatgtaa aatagactgg tcaaaatctg
      961 gtcaaaaaag aggactaata caggtagtcc tcttttttgt tattttaata ttctaaatca
     1021 ttttttattg gtttattccc ttattcgatg tagactcaaa tcactatatc actagatatc
     1081 aaacggcgtc atacggaatc aaaaacatca aatttgacct gttaaatggt cattgaataa
     1141 cattcaaaat caataaaacg gagtcagaca cggagtcaaa aacgctgaga gtaaaaaata
     1201 tatacagcct tgtaggtaaa gtaccacagg gctgtatatt ttttatttca gattacctta
     1261 aatgattcca tttacttttt atctcatttt ttcctactag ttagtatcaa atactctcct
     1321 attttctcaa taaactattt ttggatacat ggtatctgaa acagctaaaa ccttgtcatt
     1381 acagcaaaaa atcacgttat ccagactgta gacaaagtcc attttatgga tgatgtctac
     1441 agtcttttct tttataatag atttatgaaa acgaatgcaa ggagtgatag ctatgatgtc
     1501 taaaaacaat acgaatggta gaaatcaatt tgcgatgctt acgattgatg atctagtt
//
LOCUS       DAZUMT010000213         1501 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000213 DAZUMT010000000
VERSION     DAZUMT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.222"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..125)
                     /locus_tag="ACG8FM_002941"
     CDS             complement(<1..125)
                     /locus_tag="ACG8FM_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFS5095519.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="ACG8FM_002942"
     CDS             complement(143..463)
                     /locus_tag="ACG8FM_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5095520.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            762..>1501
                     /locus_tag="ACG8FM_002943"
     CDS             762..>1501
                     /locus_tag="ACG8FM_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS5095521.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEG"
BASE COUNT          557 a          258 c          234 g          452 t
ORIGIN      
        1 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
       61 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      241 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      301 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      361 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      421 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      481 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
      541 atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta
      601 ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt
      661 aattatgctc ttagtataga gtattactca gatactgtca attgaattta gaaaaaagtt
      721 gttaaatttg tttacgaata taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt
      781 ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca tattttgtag
      841 gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa agatgctttg tcgcggcatg
      901 tagatttgga agataagatg gggtcgcgaa tcacgacctc aggtcaatca agagagatga
      961 caatcatcaa cgaatctggt ttgtacagtt taatcttaaa atcaaaactt ccctctgcca
     1021 aaaaatttaa acgttgggta acaagtgaag tgttgccagc aattagaaaa catggaggtt
     1081 atctaactcc agaaaaagta gaagaagctt tgcttaatcc agatacaatc attcaattag
     1141 caactcaact aaaagaagaa agaactggaa gattaatcgc agaacaaaag attgcagagt
     1201 acgaacctaa aatctcctat ttagatagca tattatcttc tacagattca gtaacaatta
     1261 gtcagattgc agcagattat gggatgtctc cacaacagat gaataaatta cttcataaac
     1321 taggtattca gaaaaaagtc ggtaaccaat ggttattgtg caaaaaacac atgaaccaag
     1381 gatacacaaa atctcataca actgagatcc cgaaagccga tggtggcact aaaattgtaa
     1441 tgaataccaa atggacacag aaagggcgtc tatttatcta cgaattacta aaaaaagaag
     1501 g
//
LOCUS       DAZUMT010000214         1448 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000214 DAZUMT010000000
VERSION     DAZUMT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.223"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1192
                     /locus_tag="ACG8FM_002944"
     CDS             <1..1192
                     /locus_tag="ACG8FM_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5095522.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
     gene            1326..>1448
                     /locus_tag="ACG8FM_002945"
     CDS             1326..>1448
                     /locus_tag="ACG8FM_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HFS5095523.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYL"
BASE COUNT          577 a          206 c          306 g          359 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa aagaggaaga atctttttct tcctcttttt gttttttatt
     1261 ttgggagata ggttgttagt taaaaatgaa gatacttttc atagaaggga aaagaatgtg
     1321 attaagtgaa aaaaatagta attattggat caaaccattc ggggctagct gcagctcgat
     1381 ttctaaactt atgtgattca aattatgaag ttattctgat agataaaaaa tcagaaatta
     1441 gttactta
//
LOCUS       DAZUMT010000215         1424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000215 DAZUMT010000000
VERSION     DAZUMT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.224"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            313..855
                     /locus_tag="ACG8FM_002946"
     CDS             313..855
                     /locus_tag="ACG8FM_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095524.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1011..>1424
                     /locus_tag="ACG8FM_002947"
     CDS             1011..>1424
                     /locus_tag="ACG8FM_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS5095525.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMA"
BASE COUNT          543 a          173 c          278 g          430 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa gttcttcgtg ttctcttaga
      181 cataaaaatt cctcctggta tgttttcttc tagtctacac accttaattt ttctgtctag
      241 ttaattgtag cctatccagt ttggaaaaat aaaaaattat atttcatcaa cgtttcataa
      301 aaggtgtgta gaatgttatt gaaagtagct ataaaatgct attgtgtaaa aaataaagga
      361 ggaataattt atgaaaaaat agtaccaaga ggagcatgcc tattacttgc attatgtttg
      421 agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca ataaaataaa agaaggacaa
      481 tccataagtt ctgaagagga aggcatgtca attaatgcaa atggcaaaaa tgaaactttt
      541 aagccatcag attatacatt ggaggcaaag aaagaatatg tttatgaata tttagggctt
      601 aaatttaaac tctcagacaa atttagaaat tatatagctg ataaaaagat agcgatgtta
      661 gatgatcaaa gtcctataga taaagaacta aagtatgcta ttttgacatt tgagaaaatg
      721 acagaagaac aaaagaatgc agttatagaa aagatgggcg atggatataa aaattggcaa
      781 aatgagcttg aaagaattgg gactattggt atatttgaaa aaaatacatc agaagaaaaa
      841 aatctaaaat tataaaatgt gatactcata ctaaaatagg agtttcaagt gatggaaaat
      901 acgattgcta tttaagcaca aatagcggag ctgaaagtaa tcttctggat gaattaaaaa
      961 gaactgagat tcaaattata gataaaaaag aacgtcctaa aaatggtttt gtgctatcag
     1021 aaaaaactga tttagaaaat acagaagcat ttaacaaaga atcggttaaa gatttacgta
     1081 aactaccaac aaaggacata aatggcaagg attttacaag taaagatttt gagaagtatg
     1141 atcttactat ggtaaatgta tttgcaactt ggtgtacagc ttgtgtaaag gaaattcctg
     1201 atttggttga ggttcagaat gaaatgaaaa gcaagggtgt aaatatcgtt ggagttgtta
     1261 cagatcctgt tgatgataac ggtgaaaaca aggaagcaat tgaaaagtca aagttaatac
     1321 atgaaaagac aaaggcttca tatcctttct taatgccaga tggaatggca acactttttt
     1381 tgacaaaaat tataaggtgt tgtgaatgct gcggtgttat ggct
//
LOCUS       DAZUMT010000216         1417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000216 DAZUMT010000000
VERSION     DAZUMT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.225"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..722)
                     /locus_tag="ACG8FM_002948"
     CDS             complement(<1..722)
                     /locus_tag="ACG8FM_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095526.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRV"
     gene            complement(824..1177)
                     /gene="tnpB"
                     /locus_tag="ACG8FM_002949"
     CDS             complement(824..1177)
                     /gene="tnpB"
                     /locus_tag="ACG8FM_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5095527.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(1167..1361)
                     /locus_tag="ACG8FM_002950"
     CDS             complement(1167..1361)
                     /locus_tag="ACG8FM_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095528.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          398 a          287 c          249 g          483 t
ORIGIN      
        1 actcttaatc gttctcctaa agggtctaac gcgttttgca ctgctttaat atgccaattg
       61 gtgatattat ctctcgaaat atccaaccca tgtgtttgtt tccaatagtt ctcttgccga
      121 taggcaggaa ctttctgacc aaatttcatc cggatcgttt ccgccactaa actattggaa
      181 cctaagctat tagaaatcaa aggtttagga acgatcgctt ttttaaaaga agaaagtcct
      241 tctttttcgc agcttttaca acaataggta tgttgataat gggtgagaca atccaacata
      301 gcctgatgga aacgaatttc ttctcgtact tttgtccgtc cgatatcttt catctgtgaa
      361 ccgcaatgtt cacaaaattg ttcttccaaa ggaaggagat gttctttctc tgttataggt
      421 aagtgtttga ttttgtctgc tttcgttcct acaactcgtt gatgtccttt tactggaata
      481 atggtggcag ctgcttctgg aacacttaca gaaatttctt catcactaaa aagattcaat
      541 tgcccattcg tttcctcaaa gagagtctct ttagagcgtc caaataattt gttggttaaa
      601 taattgacct tttcattcaa tagttggatt tcagttgtaa gattctgaat ggtttgttgt
      661 tgtgcttgat tggttttatg ggcttcttga agcaactgaa gcaacgtatc tgtagtttcc
      721 aaaatttttc accaccttga atcgaactga ttttgttaca gaataccaca agaaacgcac
      781 aagaaatagg ggttcaacac attttttgtg ttaacccccc agcttaaaaa actccttttt
      841 ttcctggcaa aatagctcga ggttgatcga tacttaatcc ttctaacaac catcgaacct
      901 gttggttcgt taaggttcga acttcgtctt tcgtccgagg ccattttaac ttgccgtttt
      961 ctatccgctt ataacagaga agaaaaccat caccgtccca atagagaagt ttgaaacggt
     1021 cttgacggtt cccgcaaaat aaaaaaacgg catcttcgta aagctcgagt tgatactctt
     1081 gttgaataag actggcaaga ccatcaattc cttttcgcaa atctgttttt ccgcacacaa
     1141 tataaatatg tttggtttga agtaaatcat aaagcattgg acaacagttc tttcaaaata
     1201 gcttgaagaa tataattgtt aataccgtta tagatcaaaa cttcagaacc attctctctt
     1261 ttgatgcgca tcgctaactt ggcagccgcg ggtttacctt tttctggtgc actcttttcg
     1321 tttcctaatt ggacaggaac aatctcattt aatttataca tagaaaacct ccagacttct
     1381 tgatagctca agtatatctt gaggtgcatt tgttagt
//
LOCUS       DAZUMT010000217         1391 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000217 DAZUMT010000000
VERSION     DAZUMT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.226"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..416
                     /locus_tag="ACG8FM_002951"
     CDS             6..416
                     /locus_tag="ACG8FM_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5095529.1"
                     /translation="MIKVYTSPSCTSCRKARAWLKANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            430..786
                     /locus_tag="ACG8FM_002952"
     CDS             430..786
                     /locus_tag="ACG8FM_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5095530.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          511 a          207 c          259 g          414 t
ORIGIN      
        1 agaaaatgat aaaagtatat acttcgccta gctgtacttc ctgtagaaaa gcaagagcat
       61 ggttgaaagc gaatcaactt gaatttgaag agaagaatat ttttgccgaa ccgttaacag
      121 aaagtgagat caaaaagatc ttggcagcga ctgaaggagg agttcaagat attatttcca
      181 ctcggtcgaa aatttacgaa aagctgggca tagattttaa cgaattaact ttaaagcaaa
      241 tgatcgtctt attcaaagaa tatccttcat tattgagacg acctattttg attgatgaaa
      301 aaagaatact aatcggattt aatgaagatg agatccggtc atttgtccct cgtgaaattc
      361 gatgtgttgc cagaaatctg atctatttac aaaacaataa catcttaaat tcatagggag
      421 ggagccttcg tggaagtgaa agtctatgga tcagcgcact gtgtaacaag tctgaaggcg
      481 aaacaatggt tagaacacca tcaattgcct tatcaatttg ttgacttaga agaacgcgat
      541 ttaagtacag aagaagcaga agaattatgc agttttgaag aagtgcaagc agaacagcta
      601 tttgcgactt ggtctgaggc ttttcacaac attgcgattg acctttcctg tgtaggaaaa
      661 aaacaactga tttttttatg cacacaacat accaatctcc tcagacgtcc tttgattatt
      721 atcgatgaag ttctatttat tggatacaat gagcagttga tggaacagct tatcttaaat
      781 aaatagactt taactgtttt aatcaagtca ctgagcattt catacagttg taacagtcga
      841 acacttagca tcaagcgtgt tatgtgttct ttttttgact ttatttttct actatagaga
      901 catgctcaat ttatgtggtc aaaattcaaa tctaggtggg gatatagagt taaaaatata
      961 ttgacatgtg taaatttaca tgatatattc atttctgttg ttttaagtag caaagcaata
     1021 aaatagtatt ttaaaaaaca tataaaatag ttgttgacat gttagaaatg acatgataag
     1081 ataactgagt tgttaaaaaa gaaccacttg tgaagcttga ttctatcgaa aaaaacttca
     1141 aaaaagttct tgacaaataa caaatgataa gttatgataa atgagttgct gtaagatttc
     1201 gaaaaacgaa atcaaaaaaa cttttaaaaa agagttgaca attacttgtc tctctgatat
     1261 gatataaaag ttgctacggc agccaaaaac gaatcgggga acactcctga tgaagtagac
     1321 ctttgaaaac tgaacaaagt aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa
     1381 gacaacaaac a
//
LOCUS       DAZUMT010000218         1374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000218 DAZUMT010000000
VERSION     DAZUMT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.227"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1372
                     /locus_tag="ACG8FM_002953"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACG8FM_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS5095531.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAZUMT010000219         1359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000219 DAZUMT010000000
VERSION     DAZUMT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.228"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..>1359
                     /locus_tag="ACG8FM_002954"
     CDS             102..>1359
                     /locus_tag="ACG8FM_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5095532.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLR"
BASE COUNT          428 a          270 c          259 g          402 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1141 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1201 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1261 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg
     1321 cgtacggctt tcgcaacttt tacaatttca aactacgaa
//
LOCUS       DAZUMT010000220         1345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000220 DAZUMT010000000
VERSION     DAZUMT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.230"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1295
                     /locus_tag="ACG8FM_002955"
     CDS             <1..1295
                     /locus_tag="ACG8FM_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095533.1"
                     /translation="PEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEIVDS
                     KVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYFG
                     VFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPIG
                     KRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAFG
                     LAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFDF
                     EMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVGI
                     CPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSDCLLE
                     KFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          468 a          198 c          244 g          435 t
ORIGIN      
        1 agccagagat ccaaatttca gaagaagcag agatccatgt cgaacgtctg aatttgacgg
       61 atatcaaaaa tttaagaaga atctactttc aacgatcaaa aattgctcga ctgttaacgg
      121 agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga gaatcatttt ttaagcatat
      181 caagagccta tgtaaaaaga agagaattag tctcttttct tgaacagcat caaataaagt
      241 taaagaaaaa catattagtt ggggaagaaa taaacatacg gaacgtatta tttggggtat
      301 atttcggggt ctttaatggt ttttctatgc catttagaga aagcgtcatg aatgaagcaa
      361 agagtatcgt gcaatatttt gaatatctgt tcaatttaaa actgtctgca acaaaacgaa
      421 tcaaattcaa tttgctaacc actattagtt tgttgagggc ctatgggaat caaccaatcg
      481 gaaaacgttt cttttcagat gaatacttta agagctcaga aggaatgaag atcatcaagg
      541 atttatcaca tatttctttt cttcatatcc ctaaagagca gatcattgat gagatttctt
      601 atatcatgtt gtttttgatc acggaagaag aatttgaatt agatggcgca tttggacttg
      661 cggaagctca ttttcatgat atccacatga ttagtcaaaa aatgtcagtg gaattgattg
      721 atcaattacc attttcgatt gaaatctctg aagaagaaag actatatata cgaaaagaac
      781 ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat attcgacttt gagatgacta
      841 gctttacgtc taaaaagcag ctacagtttt tcttcgagac gtatccaata ttcagtacag
      901 caatccaaaa ggtcatacaa gagaataaac gctatttctt gggaaaagat gaacgtttca
      961 tgattcgctt attttatgat tatctctttt tattggtcgg tatttgtccg gtatcatatt
     1021 ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg atctgcttat accgattaca
     1081 ttatttctca agtgaaagga tttaaaaatt acaatattgt gattgagagt cgaatcactt
     1141 ctcgtacgaa tatttattta tctgattgtt tacttgaaaa atttaataaa gaacagataa
     1201 tttggaaaaa tccaccgact ccagatgact gggaatattt tggaaatacc atcatcagag
     1261 aaaaaaatag acatttaact gaaaaaatca gctagtattt atggatggca gctttgtctc
     1321 acatatttat tagctgattt atgtt
//
LOCUS       DAZUMT010000221         1318 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000221 DAZUMT010000000
VERSION     DAZUMT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.232"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1318
                     /locus_tag="ACG8FM_002956"
     CDS             82..>1318
                     /locus_tag="ACG8FM_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS5095534.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFK"
BASE COUNT          447 a          257 c          251 g          363 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaat
//
LOCUS       DAZUMT010000222         1306 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000222 DAZUMT010000000
VERSION     DAZUMT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.233"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1306
                     /locus_tag="ACG8FM_002957"
     rRNA            <1..>1306
                     /locus_tag="ACG8FM_002957"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          348 a          287 c          398 g          273 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatgg
//
LOCUS       DAZUMT010000223         1304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000223 DAZUMT010000000
VERSION     DAZUMT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.234"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..>1304
                     /locus_tag="ACG8FM_002958"
     CDS             55..>1304
                     /locus_tag="ACG8FM_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095535.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFEN"
BASE COUNT          440 a          166 c          175 g          523 t
ORIGIN      
        1 gactaatcta gtgtttaaaa aaaataaatg atactatatg taatataaaa aaacatgagg
       61 tggtatatga atttgaaaaa aattttttct gtaaaaatat ttatagttta tgcatttgtt
      121 agtttattat atttatttcc agcatttaaa aacatggaaa tatttggctt ttatgatacg
      181 gcatttcaca ttaatcgtgc gttaagttta gaatctattt ttagctcccc tattaatttt
      241 gaaacgttta gatcatatgg catgcaagta aataattttt acccatggtt aactctatat
      301 cctttatttt tattgatcaa gtttactaat ttagcaatag gctataacct ttttctttat
      361 attgttacgc tcattactct atttatatgt cattatgtaa tgtatgaaat taccaaaaaa
      421 catgttacta gctccttttt cgctattata tatacaacat cttcttttag gagtgtagaa
      481 atatttcttc gaggggcaat gggggaatta ctcgcaatgt caatacttcc tcttatttta
      541 ctcgggttta taaagcttta cgactcaaaa aaagaatctt gggttatgtt ggcgattagc
      601 atgactttat taatttacac tcatgttctt tctgtagcta tgactatggt aatgattata
      661 attgctttaa ttattcaatt ttatcggaaa aagaaaatag aaatcttctt aattattaaa
      721 ttaataaaag ctgctatagt tactttactt ctttcactag cgtttatagg tcctatgatt
      781 gagcagacat tgtatcaaga tctgaataga ccttatgtgg atatattaca aaaaagagca
      841 atttatctgg gtaaagagtt tttgattgga agtatagatt cagaattact atcatttggt
      901 attggcttaa ctttgcttat ttgtttaatt atactaacat ttaactttaa aaaattagga
      961 atcttatcta aaatcactta tataatgggt ataatatcta ttctactttg tacaaaaata
     1021 tttccatggt ttgccttaca aaatactcct ttaaatatta ttcaagctcc ttggcgtttt
     1081 atgatatttt caacattgtt catatctttt tcagtatctc tatttatttc tccacagttg
     1141 gaaaaatttt cttatacaag gagaagaaat tttatactat tattaatatt agctatttca
     1201 attttaaatt attcatcagt agttaacatg gtgaaagaaa tgaattttca aggaacttat
     1261 tctaatgaac aaatggaaaa taaacttgaa gaatttgaga attt
//
LOCUS       DAZUMT010000224         1277 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000224 DAZUMT010000000
VERSION     DAZUMT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.235"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..532
                     /locus_tag="ACG8FM_002959"
     CDS             35..532
                     /locus_tag="ACG8FM_002959"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5095536.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="ACG8FM_002960"
     CDS             complement(569..1252)
                     /locus_tag="ACG8FM_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS5095537.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          445 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaaa
//
LOCUS       DAZUMT010000225         1273 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000225 DAZUMT010000000
VERSION     DAZUMT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.236"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..1247
                     /locus_tag="ACG8FM_002961"
     CDS             15..1247
                     /locus_tag="ACG8FM_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS5095538.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          411 a          246 c          289 g          327 t
ORIGIN      
        1 gaggaatttt tattatgtta tctgatttta atgaacttct aaaaaaatat gcacgcttaa
       61 ttgcagaaac tggcgtagcc acagaaaaag gacacacagt tgttttacag atcagcgtgg
      121 aacaggcacc ccttgctcga ttgatcacac aagaagctta taaactagga gccgctgagg
      181 ttatcgttca atggactgac gaccagatcc aaagagaatt tcttttacat gctgcaacgg
      241 atcgtattga gaatgtacca caatctaaaa tcgatcaagc ggatgaatgg cttgaaaaag
      301 gagcaagcag gatcagcgtt gtttctgccg atcccgatgc atttgctggt gtagatagcc
      361 atcgagtagc gtcttatcaa tcagcgaatg gcaaagcact aatgaattta cgcaaggcaa
      421 ctcaagcaaa taaagtcagt tggactgtcg tggctgcggc tggtaaacaa tgggcagcaa
      481 aagtcttccc agaactgcca gaagaaaaac aagttgaggc attatgggat cagattttca
      541 aaacgactcg tgtctatgaa gaagaccctg tcttagcatg gaagaagcat gatgaaaaat
      601 tagcaaaaaa agcagaagaa ttgaatcgag aacaattttc tgcccttcac tatactgctc
      661 ccggtacaga tatcattatc ggattgccta aaaaccatct ttgggaaggc gctggaagct
      721 ataatgctcg tggagaaaaa ttcatggcaa atatgccgac ggaagaagtg ttcacagcac
      781 ctgacagtca tcgagtagat ggttacattt caagtacaaa gccactgagt tatgctggca
      841 ccatcatctc gggtatgaaa tttactttta aagatggaaa agttgttgat ttttctgccg
      901 aacaaggaga agatgtctta gcaaaactgc ttgatacaga tgaaggtgcg cgtcgtttag
      961 gagaagtcgc acttgtacct gacccttcac ctatttctca atctggcatc atctttttca
     1021 atacattatt tgatgaaaac gcatcgaatc acttagcact aggttctgct tatgccttca
     1081 gtgtgaaagg cggtacagaa atgtccgatg aagaattagc agaagctgga ttgaatagaa
     1141 gtcaaaccca cgtagatttc atggtaggat ctgacaagat ggatatcgac ggaatccgtg
     1201 aagatggctc tacagtaccg attttccgaa atggcgattg ggcttgagta tactttcgca
     1261 cacatgcaaa aaa
//
LOCUS       DAZUMT010000226         1246 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000226 DAZUMT010000000
VERSION     DAZUMT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.237"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..726
                     /locus_tag="ACG8FM_002962"
     CDS             <1..726
                     /locus_tag="ACG8FM_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5095539.1"
                     /translation="TTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFW
                     NIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEK
                     WNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQT
                     MTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTK
                     IKTELTRQMKRDAGAVGGTLYAT"
     gene            716..>1246
                     /locus_tag="ACG8FM_002963"
     CDS             716..>1246
                     /locus_tag="ACG8FM_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5095540.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKD"
BASE COUNT          475 a          153 c          229 g          389 t
ORIGIN      
        1 actacttgga ataatattaa acagtttttc aaagatactt ggaaaaatat taaagatacg
       61 gcatacaata cgtggatttc tataaaaaat accatgatta atacatggaa taacattaag
      121 gactctttct ggaatattgt tactggaatt gttaattctg ctgaaaatgc atggacaaat
      181 ctaaaaaatg gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa ttcgttaagg
      241 gaagtcaatt tatttgaaat tggcaagaac atcattgatg gacttataaa tggtgtaaaa
      301 gaaaagtgga atgctttgaa agatactatc aaaggtattg caggtaatat caaaaattgg
      361 attaaaggag ctttagatat tcattctcca tcaagatgga tgcgtgatat ggttggtaaa
      421 aatattgttc aaggtattat cgttggtatt gataaagagc aaagtaagtt agatcaaacc
      481 atgacaaatt tagtaaaaac accgtctgtt caacctgtag taaatggaat aagtcaccta
      541 ccaattacac aaacaaagca aaatatatct gctagtgaaa caaaagaaat tcatttacat
      601 ttaaatgttt atggagattt acctgattca atggttcgga agattaccac aaaaattaaa
      661 acagaattga caagacaaat gaaacgagat gctggtgcag taggaggaac attatatgca
      721 acttaagaga ggacaatttt ttattaatca acattattct tctgaattta atgtgtatat
      781 tcaaaacagg cctgcttctg tttcagctag tcgagtaatt gaattgagag agcgagaagg
      841 aaatgattct atcattattg ataaaggcta ttacaaaaat gtgactagaa agattgagtg
      901 ttactataaa gcaccttcaa ttgatatggt gcaggagtgg gaagatcgaa tcactgaatg
      961 gttagatatg tcgtcttata gtgattttat tctttactat gatgaacaat atatttatca
     1021 agctgtagta atagaaggac ctgaatttaa aggaacaaga aaaacaggaa atattgtccc
     1081 ttttgaattt acagtgagta ttcgaccatt taaggaaaat tacaatggta gatttactat
     1141 tcgacaaaca gaaacttttg aaatttataa cccagagaag tattcttcaa aaccgcttat
     1201 taaattgagt ggttctggag atgcttcttt ttatattaat aaggat
//
LOCUS       DAZUMT010000227         1197 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000227 DAZUMT010000000
VERSION     DAZUMT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.238"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..>1197)
                     /locus_tag="ACG8FM_002964"
     CDS             complement(14..>1197)
                     /locus_tag="ACG8FM_002964"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5095541.1"
                     /translation="NLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGT
                     RFSQEYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIG
                     AGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESP
                     KKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQT
                     NIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGF
                     SIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIV
                     GVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIE
                     T"
BASE COUNT          390 a          198 c          145 g          464 t
ORIGIN      
        1 catttagtaa cgattatgtt tcaatgttta atcctgttac tgcctttaaa ataaaattat
       61 agggttggct aaaactagga tgaaataaat aatctaacat tttcaatttg tctaatgtaa
      121 tttgttcttc tatagcaaga gaaaacattg ataatagtcc agataaatct tcttgactac
      181 aaagttgtac acctactatt ctctttgtat ttttttcaaa gctaattctt aattttacac
      241 gactattttt tctcaaaaaa acaggtctaa tgaaatcttc tatttctata taatctatag
      301 actcacttaa taatcgtgct gtttttaaat tcaaacctgc tgcgtaaaaa ttgtaattaa
      361 atatagaaaa gccatttgta aattgggaac cagaaggtgt aaaatcttga ttgtcaatat
      421 gttgagttgc aatataagct gttcttagag cattagaaat agaaaaatca acactacttc
      481 tattaatgga cgtaaaaaaa gttgtagcac aatccccaat agcatatata tcttttatat
      541 ttgtttgctg gtattcatta gttagatatg ctccactaac atctctcaag agttcctttt
      601 tgccaattgg actattagat agaaatccta tagcttgtac gaccatatcc atagagtatt
      661 gggtaccact tgtagtgact attttttcaa tcttttttct tcctaaatat tttttaggac
      721 tttctgatag acaaagatgt attccatttt ttgatagtat ttcttcaact ttatctgaaa
      781 aactacggtc gtaataagtc gacaacactc tatctgcaac atcgaaaaga tatacttctt
      841 tccctctttt tcttatggct tcagcaattt caactccaat atatcctgct ccaataattc
      901 ctactttttt aatagtatta tcatctaaag ctttaaaaat ctctttagca tttggaaaat
      961 tctttatcga aaaaatatta gctaaattat tccccggtac atctaacata atttgctttg
     1021 aacctgtagc tagtataagt ttgtcatatt cttgggaaaa tcttgtgcca tcttttttta
     1081 cacaaataat ctttttattt tgaatatcaa ttttgtcaac tgctgtctcc aaaaacaact
     1141 tttttatctt agaatttagt tctcttctat tttgataaaa aagatcttct aaattgc
//
LOCUS       DAZUMT010000228         1195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000228 DAZUMT010000000
VERSION     DAZUMT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.239"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8FM_002965"
     CDS             <1..1076
                     /locus_tag="ACG8FM_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5095542.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZUMT010000229         1187 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000229 DAZUMT010000000
VERSION     DAZUMT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.240"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..148
                     /locus_tag="ACG8FM_002966"
     CDS             <1..148
                     /locus_tag="ACG8FM_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS5095543.1"
                     /translation="QCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNY
                     LYRR"
     gene            153..1100
                     /locus_tag="ACG8FM_002967"
     CDS             153..1100
                     /locus_tag="ACG8FM_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5095544.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          386 a          172 c          253 g          376 t
ORIGIN      
        1 gcaatgtggg ataataaaaa tgagagatta tctaatttta gatattgata gtgaagaaga
       61 ttttaaatgg ttagaatttt tgtttcctaa atttttagaa gattcttcgg agcttcaaga
      121 tatctataat tatttatatc ggaggtaaaa ggatgagctg ttatcttatc accggtgggg
      181 ctggcttcat tggttctacc ttagccaatc atttaggaga agaacatgtg gtaatcgtcg
      241 tcgatgactt atcaatggga aaagtagaaa atcttgatat gacgaaaaat atcactttta
      301 ttgagggaga tgtcgctgag aaatccttga tggaagagat catgaaagct aataaatttg
      361 attatatctt tcatttagca gcagttgcta gtgtggcaga ttcagttgcc cgaccagtag
      421 aaacacatcg tgtgaatttt gaaagtgtat taatgatttt agaattgatc cgcaagtatc
      481 aacctgattt aaaacggatc gtgtttagtt cttcagcggc tgtttatggg gatgaaccga
      541 ctttaccaaa aaaagaagag tccgtgattc gcccattgac gccttatgcg gttgataaat
      601 tcgctgcaga acaatatgtg ctagattatt gtcatttata tgatgtaccc ggtagcgcag
      661 ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa ttctccatac agtggtgtga
      721 tttctattct cgtagatcgc tataagaaac aaatcgtagg agaagcaact tcatttacat
      781 tatttggtga tggaagccag tctagagact ttgtttatat agatgatgta atccaagcat
      841 tacttttagt tgcgaatgag gaagctgcac tggggaaaca attcaatgtt ggaacgggta
      901 aagcaaccac acttttggaa ttgattcatg caatcaatga aattctcggt gtagaattga
      961 cacttgaata caaagaagag cgttcgggtg atatccatga ctctctagca gatattacta
     1021 agatccagtc aattggctat caaccgaaat atggtatttt aagtggaatg actaattatt
     1081 tgaaaactga aatcaattaa aacatactaa gataagatta gagggtaaat tcaggctctt
     1141 tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg gatagaa
//
LOCUS       DAZUMT010000230         1141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000230 DAZUMT010000000
VERSION     DAZUMT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.241"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..298)
                     /locus_tag="ACG8FM_002968"
     CDS             complement(<1..298)
                     /locus_tag="ACG8FM_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095545.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENY"
     gene            complement(300..>1141)
                     /locus_tag="ACG8FM_002969"
     CDS             complement(300..>1141)
                     /locus_tag="ACG8FM_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS5095546.1"
                     /translation="VLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLF
                     LLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVE
                     VIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYG
                     NSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLL
                     KTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLK
                     KEN"
BASE COUNT          439 a          193 c          134 g          375 t
ORIGIN      
        1 aataattttc cccctgtata aaaatttcat cataaacgga taaatcatcc gagaaaaatt
       61 gattatggtt agttaacaag ttaattttac gtttaaagga agaaccgatt ttccggtgat
      121 tcacaaaaac aacatcatta aatagcccta attcttttat acgctcacta tatcttctag
      181 cgttttcaaa ttcagaataa ataaataaat ctccttttga ttctcggtaa aaattttgat
      241 aaacatttaa tatattaatc aaatgtaaag gagtccaagc aatgaaagct gtcttcatat
      301 taattctcct tttttaaata caagtaagaa atgcttccca aataatacca gaaaacaatt
      361 gaaatgacat cgcgcttatt gaaaagtaca tgagcctcca ccatttcatc tataaaaata
      421 gatagtataa aaattaaaac aataccaaga aacgcataat tcactttact tctaaaaaat
      481 gttttgagta aactaaagat atttaacaca tagaaagcat agatacatat aaaagcaatt
      541 ataccacttg atacaagtac ttgaagaaat ccattatgca tgtttccatg tgctcttttt
      601 agttcattca catctgtagt atcaagatct acttttttca aaaattttgc atcgctattc
      661 ccatagaaat cggtctctcc ataaccaaat aacaaagttg gtcttataac tttaagcgca
      721 gctttccaaa tcgtaaaacg accagctgaa atttcggagt tatcactaga atgctgtaat
      781 attacttctt tttctggaag tggagtttta tctgattcat ttgactctat aacttctact
      841 tttctatcaa tcgatacagg aattgttgct aaaaaatcct tagcataatt agtaccaaaa
      901 gaaaaatata ccaatagaac taataatatg cttaccatcc atttgatttt tacaggtagt
      961 acttctctaa tgctctttct aaattttgaa ttggcaaaca acaaaagaaa taatactaaa
     1021 aaagaacaaa ttactacctg ggcacctcta gacgaagaaa gtgtaatata aaatatttgt
     1081 acgataaaat tcatcatata aaaaacacga atcgtttttg ttatttttga aattaataca
     1141 c
//
LOCUS       DAZUMT010000231         1128 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000231 DAZUMT010000000
VERSION     DAZUMT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.242"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..720
                     /locus_tag="ACG8FM_002970"
     CDS             <1..720
                     /locus_tag="ACG8FM_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS5095547.1"
                     /translation="QIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNL
                     SNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSL
                     PEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSE
                     TKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKK
                     YAGVSPKQYQLGLVDNFNNIS"
     gene            865..>1128
                     /locus_tag="ACG8FM_002971"
     CDS             865..>1128
                     /locus_tag="ACG8FM_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095548.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNN"
BASE COUNT          422 a          123 c          198 g          385 t
ORIGIN      
        1 caaatcaaaa tagataatct gttatcacaa aattacgatc cagaaatata tctgtttctt
       61 tatgaaaata aaatactgga atatgttaaa aatggagata ttcgaaattt agaaaacatg
      121 gtttttaatc tgagtaatgg aataattcca agtgttagtg gagatacgat tcggtctgaa
      181 aaaaattatt ctatcattgt ttttgaaaag ttagcacaaa cgtctataac attaggaatg
      241 gatataatag aagcgtatca aagtagagat gctttgatac aagaaaacga attagctgta
      301 tcgttacctg aggtcttaaa agtgagggat tctgggattg tttattatac taaggaaatc
      361 ggaaaaacta agatagagca tctttctccc ttaatttctt ctgtcgttca attcatcggc
      421 ttaaatatct ataaaagaat tactgtaaag gaaatagcta attatttttc tgtaagtgaa
      481 actaaattaa gagaaagctt taagaatgaa atgcatatta cgatttataa ttatatctct
      541 aagcgaaaaa tttctacggc taaaataatg ttgaaatcaa accatactat cagtgaagtt
      601 tctttaggtt taggattttc agattcttca cacttttcga gagtattcaa aaagtacgca
      661 ggtgtatctc ctaagcaata tcaattaggt ttagtggata atttcaacaa cattagctga
      721 gaggttttat tttattatga gactatatca tttacgtagt ttggaggagt tgaggaaatt
      781 ggattttaac aaaaaatttt ttgggaaatt tctgatttga gttgtttcgt agaatctgag
      841 cgtctaagta atggtaataa tagtgtgtta aatgaatatg acaaagtagt aattaataaa
      901 gtaaaaagtt tatgtgaaga agctagagaa tctatcagtc atattaatgg tagcaatttt
      961 aagcaaacat tatttaatct gattaagcta gatgctaaaa tatcaagtta tttattcttt
     1021 ttaatacatg atgaatttat ggattatcaa aaattggatc agttaattga aagagaaagc
     1081 tgggaagatt attacgtggg tctaactttt tcagaaaagt taaacaac
//
LOCUS       DAZUMT010000232         1123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000232 DAZUMT010000000
VERSION     DAZUMT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.243"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG8FM_002972"
     CDS             <1..963
                     /locus_tag="ACG8FM_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5095549.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          383 a          209 c          224 g          307 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gat
//
LOCUS       DAZUMT010000233         1122 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000233 DAZUMT010000000
VERSION     DAZUMT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.244"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..945
                     /locus_tag="ACG8FM_002973"
     CDS             <1..945
                     /locus_tag="ACG8FM_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS5095550.1"
                     /translation="INDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNVAANMKNNY
                     NGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRA
                     YIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKE
                     KLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHV
                     AGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNF
                     PSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          382 a          194 c          227 g          319 t
ORIGIN      
        1 attaatgacg agtataattt agcgccgaat caaggagact ccagattagc aattcctaac
       61 aaaattattt tgcacgaaac tggaatagat gcaccagcaa gaaacgtagc cgccaacatg
      121 aaaaataatt ataacggaag caattcttat actacagatg ttattggtga cggtgggatt
      181 gtttaccgtg tgggtgagca aggatatgtt tcgtggggag ctggtaacgc taatccttat
      241 gcgcctgtac agattgaatt acagcgcaca tatgataaag cattgtttga aaaaaactat
      301 cgagcttata ttgaatatac aagagatagt gcaaaaaaat atggaattcc attgactctt
      361 gatcaaggaa cttctttatt tacaaaagga atcatttctc atttgtgggt gacaaattat
      421 gtttggggga accacacaga tccatatggt tacttatcgc aaatgggagt tagcaaagaa
      481 aagcttgctt atgatttagc tcatggattt accgatgaaa atccaacaac ttctgaaaac
      541 aagcctgtca ttgatccaac acgagctggt gcagctaatc ctacgctgac agatggaaca
      601 aatcacgccc acattgatca gtttggggaa atcgaaaacg caaacttgca tgtggctgga
      661 tggcacattg ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa
      721 gaattagctc gagtaagagc tgatggaatt tatagaccag atgtaaacca agcttataat
      781 acttctggaa atgttggtta tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa
      841 gtctatgtaa tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa
      901 gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt
      961 gaggagcagt acataactat attgacaact ataaaaatta ttcgataaaa tagtgatgtt
     1021 atcgcatatc ttcactatca cccataaata gtcacactcc aagctatgcg ataacaggtt
     1081 tgttgccaca cattctactg gttgattgtt tatggcttta tg
//
LOCUS       DAZUMT010000234         1121 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000234 DAZUMT010000000
VERSION     DAZUMT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.245"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..726
                     /locus_tag="ACG8FM_002974"
     CDS             <1..726
                     /locus_tag="ACG8FM_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5095551.1"
                     /translation="LFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQ
                     MIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEK
                     LKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSAS
                     VPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYN
                     GTVTFTKLAKAEQYRRSLELSKD"
     gene            798..>1121
                     /locus_tag="ACG8FM_002975"
     CDS             798..>1121
                     /locus_tag="ACG8FM_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095552.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNV"
BASE COUNT          489 a          131 c          189 g          312 t
ORIGIN      
        1 ttattcaaga atgacaatgc tgaattaagg aagggtcttt tagagtttaa acttcaagaa
       61 ctagaacttc attactctta tttagtgaaa attaccgaag aaaaagaaca acaaaaagca
      121 ataaaagaac agatgataga agaggaaaaa gttcgtagag aaattgatcg tgaaaagaaa
      181 cgtattgata aagaacaacg tcaattcaat tctgaaattt ccaaattaat cgcatatatg
      241 caaaaagcta atgcagatgt ggaaaaagaa ttatatgcaa ataaaattca agaactggaa
      301 gaaaaattaa aagaattaga agttatacgt gaaaatgtat tacaaagaga attaaatact
      361 agagccggtt atgtgtatgt aattagtaat attgggtctt ttggtgaaga catatataaa
      421 attggtatga ctagaagatt agaacctatg gatagaataa aagaattgag tagtgcatcg
      481 gttccatttg aatttgacgt tcatgcgatg attttttcag aggacgctcc gtctttggaa
      541 actaaattgc ataatcattt tagaaaacaa gaagttaata aaattaatca aagaaaagaa
      601 tttttcaaag tttcactaga cgaaatagag aaagttgttt tagaaaatta taatggaaca
      661 gtaacattta caaaattagc taaagctgaa caatatagac gatctttaga attgtcaaaa
      721 gattaaatat ataaaaagcc ttcgggcttt ttcttttaac aattaatagg aacgtatatt
      781 cgaaaggaat agaaataatg tggttagaaa aaataaatga caaaaaattt aagtacaccg
      841 aacgttatac ggatccctta actgaaaaaa agagaaaagt gtccgttact ttatctagca
      901 attcacgcca agcttggaat caagctaact tacttttgaa tgaaaaaatc gcagaaaaaa
      961 taaaacgaaa tgaagaatta ccactaactt ttggagaatt aaaaactaaa tgggatgaaa
     1021 aatacaagcc aactgtaaaa gaaagttcct atcgaacaac tcaggtttat ctatcattaa
     1081 tttcaaaata tataaaagat gatgtacttg taaaaaacgt t
//
LOCUS       DAZUMT010000235         1115 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000235 DAZUMT010000000
VERSION     DAZUMT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.246"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..849)
                     /locus_tag="ACG8FM_002976"
     CDS             complement(40..849)
                     /locus_tag="ACG8FM_002976"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5095553.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          335 a          209 c          191 g          380 t
ORIGIN      
        1 aattgtcaca acctcttttt tgtggaatct aattctctat tatagtttaa gtagtttttc
       61 cagataaaca gctaaaccgt cttcctgatt gctcaaagga gtgacatcat ttgcaatgcc
      121 tttgagttgg tcagtaccgt tagccatagc tactccccaa cctgcataat ccaacaattc
      181 caaatcatta tgctcgtcac caaaggcaat gacgtctttt tgatcgatag acaggtagtt
      241 agcaatctct tgcacgcctt ttgctttttg gattcctttt gaaacaatct ctaagatggc
      301 agtaggtcca ccccatgtcc gaacatcgac ataactgcca aattgacgtg ttaattcagc
      361 agatacagct cctgcaaaac gtttgtctgt tcggacaagc aaagatgtag gatttgtcgt
      421 caaattttta ggagaaagca aattcttctc tcctggacga gaagaagcaa aaattttttc
      481 atcaaagaaa tcaaaactgt caataaaaaa agtatcacga ttttcagcag cgataaaatc
      541 taaatttagc tgcttttttt gcgataagat ttcaaaagcg atttcacgat tgatcaacgt
      601 ttctctttca ccatcccagt gctgattagg gatgtgtact aatgcgccgt taaaattaac
      661 catcggtgtt gttaattcta attgttggta aaactgatgg ctcattcgga atgggcgtcc
      721 tgtagcaata ctgacatgat gaccggcatt gattgctttt tttagagtct cttttgtttt
      781 agatgtaatc atcgaatctt gattgagggt agttccgtcc agatcgatgg caattaattt
      841 tttattcaat aagggtcctt ccttcctcaa ctagcatttg ttttagtcta gcatatttta
      901 ggtaattaac aagagcaatc agctattcct aacaaaaact acagttatgg ctatataaat
      961 agctaaacat aagctaaaca cgaacgttaa gtattatttt cgcttttttt gttaaaaaaa
     1021 cgttgaaaag cgaatgaaac gtctatataa tttgaaattg ttcgatggat ttgccctatt
     1081 ttacttattt tttattatta tagttttcca tcaga
//
LOCUS       DAZUMT010000236         1090 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000236 DAZUMT010000000
VERSION     DAZUMT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.247"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..128)
                     /locus_tag="ACG8FM_002977"
     CDS             complement(<1..128)
                     /locus_tag="ACG8FM_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5095554.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL"
     gene            complement(134..364)
                     /locus_tag="ACG8FM_002978"
     CDS             complement(134..364)
                     /locus_tag="ACG8FM_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095555.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(623..736)
                     /locus_tag="ACG8FM_002979"
                     /pseudo
     CDS             complement(623..736)
                     /locus_tag="ACG8FM_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          387 a          175 c          161 g          367 t
ORIGIN      
        1 agattaatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatc ccacacctta
       61 taaaaatctt tgacaaatct attcgaagga tcaaatttaa accataattg cgtctcatta
      121 aaaagcataa ccattactcc gatctatttt taaacttagt ctctacctct aatatatatc
      181 gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca ggttgtttat
      241 tttcggaaaa agctaatata gctaaaattg aaacatccat ctggaatttg tctttttcgt
      301 tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata aattcttcca
      361 tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa aggtggaatt
      421 tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc aaaaaaaatc
      481 gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct tataataaca
      541 aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc tgtatccttt
      601 gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta
      661 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat
      721 gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa
      781 aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat
      841 ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt
      901 ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt
      961 ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa
     1021 acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta
     1081 tgctttatat
//
LOCUS       DAZUMT010000237         1045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000237 DAZUMT010000000
VERSION     DAZUMT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.248"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG8FM_002980"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG8FM_002980"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5095556.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DAZUMT010000238         1026 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000238 DAZUMT010000000
VERSION     DAZUMT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.249"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..841
                     /locus_tag="ACG8FM_002981"
     CDS             149..841
                     /locus_tag="ACG8FM_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5095557.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          451 a          147 c          170 g          258 t
ORIGIN      
        1 attttttaaa tagataaaaa aggaggatta aatgagtatt tgatagtatt tatattaaaa
       61 ttacatatgt aatttaaaaa cgcttgacat tgtgtttgag atcacttact tttagcatgt
      121 aaaagtattg gattaaggag ctatgcaaat gaaaaagaaa tttattactg gtgggattgt
      181 cgttgcattt ctatgtctaa ttggctacac aggcacgcgg tacgcaaatg aaattatttt
      241 gtggggtgga gagacagata ttgatacaat caatgagaat ctgacacagc ttgatcatac
      301 attgacagat aaagagcaag cgattacaga tcttaccgct cgactagcac aaaaagaaca
      361 gacattgcaa gattcaaagg cagatgtcca agaataccaa acaaaagtaa atgaattaga
      421 aaaccaaaaa aatcaatcgg cagcagaaaa acaaaactta gaaagtcaat tagtcagcaa
      481 ggatgcacaa ctacaaagca aacaaaatga aatcaatgtc aaactagatg agatcaacca
      541 aaaaaatgat gaaatcaatc atctgaatgc agattggaca gcaagactac aagaagcaca
      601 agcaaaactt tcagaattac aaaatactgt aaatggtttg aatcaacaaa tcgcaacact
      661 gacacaagaa aaaacaaatg tagaaactca gttaacagat acacaaaaga aattagacga
      721 agcaactgct gaaaactcaa gcttgaaaca atatattcaa aagttagaaa aagcaaaaga
      781 tgaagtggaa gatacagctg ataaatctca gcaaattgtt gaagaacatt tgcgtaaata
      841 atctttctta acaagtaaac ctaaattagt aaagaagcaa cagtaatcta tactaagtag
      901 ttcagttttg gagtacaaaa tgacaaaatg tacttcaaaa tggactactt ttttcatttt
      961 aacttacata ccaaattaca tacaaaatga tttataaaat gcaattgaaa atgataattg
     1021 aatgaa
//
LOCUS       DAZUMT010000239         1003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000239 DAZUMT010000000
VERSION     DAZUMT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.250"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG8FM_002982"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG8FM_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZUMT010000240          959 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000240 DAZUMT010000000
VERSION     DAZUMT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.251"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACG8FM_002983"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACG8FM_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACG8FM_002984"
     CDS             complement(570..>959)
                     /locus_tag="ACG8FM_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095558.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAZUMT010000241          943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000241 DAZUMT010000000
VERSION     DAZUMT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.252"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8FM_002985"
     rRNA            <1..>943
                     /locus_tag="ACG8FM_002985"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZUMT010000242          940 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000242 DAZUMT010000000
VERSION     DAZUMT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.253"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8FM_002986"
     CDS             <1..459
                     /locus_tag="ACG8FM_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095559.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8FM_002987"
     CDS             473..904
                     /locus_tag="ACG8FM_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095560.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          358 a          135 c          197 g          250 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga
//
LOCUS       DAZUMT010000243          925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000243 DAZUMT010000000
VERSION     DAZUMT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.254"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..739
                     /locus_tag="ACG8FM_002988"
     CDS             <1..739
                     /locus_tag="ACG8FM_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5095561.1"
                     /translation="IASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRA
                     VMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRK
                     PMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHG
                     KCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIA
                     LLKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          345 a          149 c          179 g          252 t
ORIGIN      
        1 aattgcttct ccctcaaaag ggaaaacgga attagaagaa acaagacttt ataattcaat
       61 ggtattaggt atgcaaaatt attatcagat tgcaacgaat gtaaatctgg attgtatgaa
      121 actgaataga gctgtaatgg ctatcttcac taacagatta ggtacaaaca aaaggggcag
      181 attgagaaaa acaggcagac aacttacgac aaaggaggca acaagatatg gtaaatctaa
      241 aatgcttcgg tatgttgctg gcgagggcga acctattttc cctgttggat atatccaaca
      301 cagaaaacca atggcaaagg tgtatttagc gaattgctac actctagagg gaagaaaatt
      361 tatccacacc aatctaagtg ttgataaatt actgatgtac cagttaatga aattatcttt
      421 ggaaaatcgc acaatagaat acgcagacaa tcgaatttcg ctattttctg ctcaacatgg
      481 aaaatgtgct ataactctgg aagaatttca acaagctaat gaaatacatt gtcatcatat
      541 tgtaccaact ggagctggtg gaaatgacga ttataaaaat cttattttgg taaaagaggc
      601 ggtacaccgt ctaatacacg ctaaaacaga agaaacaata caaaaatata tagctctatt
      661 gaagctaaat aaagtccagc tcataagact aaataaatat agagtattag ctggcaatca
      721 agaattaaac ttgatatagg tctactacat aaatactttt caatataaac acgatggaac
      781 gcggagtgcg gtgaaagtcg cacgctccgt gtgaatgggg ggaaaagttt gagatattta
      841 taatatcagg aacttaccta tccatatatc aatggttttt aaccaatgct caatctttag
      901 tcttactagc gattgtggtc attgg
//
LOCUS       DAZUMT010000244          905 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000244 DAZUMT010000000
VERSION     DAZUMT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.255"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..905
                     /locus_tag="ACG8FM_002989"
                     /pseudo
     CDS             1..905
                     /locus_tag="ACG8FM_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          330 a          157 c          172 g          246 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacac
//
LOCUS       DAZUMT010000245          833 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000245 DAZUMT010000000
VERSION     DAZUMT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.256"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..783
                     /locus_tag="ACG8FM_002990"
     CDS             <1..783
                     /locus_tag="ACG8FM_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS5095562.1"
                     /translation="YRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFL
                     KEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDK
                     WFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRS
                     GFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNG
                     LNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          264 a          152 c          181 g          236 t
ORIGIN      
        1 tatcgtgtga ttgaaagtgc caaaacagat acctattact ggcagttttg tacaggaaaa
       61 gacagtcagc atcccatcgt ttattatcac cacgacgaaa gtcgtgctgg agatgttccg
      121 aaaacttttc tcaaggagtt tacagggtat ttacactgtg acggctatag cgggtataat
      181 gccgtggaaa gtgtacgact tgtctattgt ttcgcgcatg ttcggcggaa atttttcgaa
      241 gcaattccaa aaggaaagaa aaacacggat attcccgcag ctcaagcagt caaacaattg
      301 gataaatggt ttgtcctaga aaagaaatgg aaagactttt ctcctgagaa gcgattaagc
      361 tgtcggcaac aagaactgcg tccattattt attgcttttt atgagtggat ggcaacgata
      421 gatcctgttg caaaatccaa gttggatgca gccgttcaat atgcgtgtaa actacgaagt
      481 ggttttgaac ctatttttga agatggtcgt ttagaactca caaataatcg tgcggagcga
      541 aatatcaaag agctggtgat aggacggaaa aattggttgc attccacaag tcttgaagga
      601 gcacgaactt caggaatcat tctgagtgta tataaaacag cagaacttaa cggattgaat
      661 cctgtaaagt atctagagtt tctatttgat aagataccaa atcttcccgt actttcagca
      721 gaaactttag atcaactgtt gccttggaac aaggatgtac aacaacattt ttctcgtaac
      781 taaaaacgcc aaacattccc gattctattc tataggtcgg gttatttgac gta
//
LOCUS       DAZUMT010000246          819 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000246 DAZUMT010000000
VERSION     DAZUMT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.257"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            366..596
                     /locus_tag="ACG8FM_002991"
     CDS             366..596
                     /locus_tag="ACG8FM_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095563.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            602..>819
                     /locus_tag="ACG8FM_002992"
     CDS             602..>819
                     /locus_tag="ACG8FM_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5095564.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVT"
BASE COUNT          285 a          127 c          134 g          273 t
ORIGIN      
        1 agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aatttccgtg
       61 aatttcttct tatttcttgg agttaaacgc ttctgttcca gcctcttttt atctgtcttt
      121 cactaatgat cagttgtaga taagcaagtt gctttttctt acatcatacc gcccatcaag
      181 acaaacgttt ttagcttgta cgtactggac aaaaatccca ttatatcaag aaaaaacaac
      241 gttgataata cttaaaatta ttaggctaga acggactagt aagagttttt tgcccctttt
      301 ttgcccctca aatatcgctt tacagccaga acataagttc gtataatcgt tttgaggtga
      361 tttttatgat ggaagacttt attagaaaaa atatcagtga tgaatatgca gacttttatg
      421 agcaaagcag caaaaaagat aaattccaga tggatgtttc aattttagct atattagctt
      481 tttccgaaaa taaacaacct gtaactgcaa aaaaagaaac agtattctct gaaggcaaaa
      541 taaaaactcg atatatatta gaggtagaaa ctaagtttaa aaatagatcg gagtaatggc
      601 tatgcttttt aatgagacgc aattatggtt taaattcgat ccttcgaata gatttgtcaa
      661 agatttttat aaggtgtggg attcagaagt tttcttttta gcaatcgaag atagcttatt
      721 aatcaatctc tactattcta ataagaacta tttcaaaatt cctgctgcga aaactagaat
      781 gaagaaggat gtatactttt tgtttgatat cgtgactga
//
LOCUS       DAZUMT010000247          805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000247 DAZUMT010000000
VERSION     DAZUMT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.258"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>805)
                     /locus_tag="ACG8FM_002993"
     CDS             complement(<1..>805)
                     /locus_tag="ACG8FM_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5095565.1"
                     /translation="GKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPEL
                     EEWQARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTE
                     SKNRWMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRY
                     LPSKDYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFD
                     YGSAIRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYS"
BASE COUNT          246 a          172 c          150 g          237 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgcgcattct cattagagaa
       61 cactcccttt tttgtgactt ttctgaagct agagtgaata ctttcaactg cattagtagt
      121 gtacatgatt tttctaatgg cgctgccata atcaaagagt tgttcaacat gagaaaagtt
      181 tcgtttgcat acatctactg caccagaata atgagaccac cgattttgaa aactgtcgaa
      241 ggcagcgtgt gcagcgttta aagaagaagc actgtagaat tttttcatgt ctcgacagac
      301 ttccttataa tccttacttg ggaggtatcg taacgcattt cgaacaagat gaacaataca
      361 gcgctgcaca atgacagaag gaaaaattgc ttttgcgcct tctttgagac cggaaacacc
      421 gtccatcgaa atgaaaaaaa catcttctac accacgtact ttcagttcat caaatacttg
      481 catccagcga tttttagatt ctgtttgatt gagccatagg cctaaaatct ccttgtttcc
      541 tttaagatca tagccaagaa tggtgtacac agcatattct ttagcttcat agttttcgcg
      601 taaagtgacg tacatacaat caacgaatag aaatgcataa cacttggcta atggccgagc
      661 ttgccattct tccaattcag gaaggacggc atccgtgata tcagaaatca tttcgtggga
      721 aatatcaaag ccgtaaatat cttcaacggt tgcggcgata tcgcattgac tcattcctcg
      781 tgcatacatg gaaagaacct tacct
//
LOCUS       DAZUMT010000248          788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000248 DAZUMT010000000
VERSION     DAZUMT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.260"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..717
                     /locus_tag="ACG8FM_002994"
     CDS             1..717
                     /locus_tag="ACG8FM_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5095566.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          196 t
ORIGIN      
        1 ttgaaaaaaa ggattttagt aacaattggt ctagcttttt tgttagctgg ttgtaccaac
       61 gggaatgaat cgaaaaaaga atcaacaaaa gaaacagcga aagcaacagt tgcaactact
      121 acaacaacta gcacagagag caagtcaact gcagcaagta cagaaacagc agatacctct
      181 acttcgtcag agtcttccaa aacgactgaa acaactcaaa catctacttc aagttcttct
      241 gtagaggctt catcttcctc ttcagaaagc agcgaacaag ttgaacaaac actgtggaat
      301 gcagacaaat ccgcacaatt agaggcattt atgaccaaat ggggccaaac aatgggacaa
      361 caatacaaaa gttatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
      421 gctattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
      481 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
      541 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
      601 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt caacgagaca
      661 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atggacaagc aaattaatga
      721 gataaacaag gggttgtgac atgtctttta tcacaacccc ttgctcagtt tatttgttag
      781 tcttttat
//
LOCUS       DAZUMT010000249          771 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000249 DAZUMT010000000
VERSION     DAZUMT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.261"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..482
                     /gene="ssrA"
                     /locus_tag="ACG8FM_002995"
     tmRNA           116..482
                     /gene="ssrA"
                     /locus_tag="ACG8FM_002995"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          251 a          126 c          150 g          244 t
ORIGIN      
        1 aaaggggcag ttattatata aataatggga tatgattcgc ttttttattg aatttatccc
       61 aatatatgct atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga
      121 cgttacggat tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt
      181 aaaaacgtta cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc
      241 ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa
      301 ctttagtggg atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag
      361 cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat
      421 gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat
      481 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
      541 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
      601 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
      661 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
      721 ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttta a
//
LOCUS       DAZUMT010000250          763 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000250 DAZUMT010000000
VERSION     DAZUMT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.262"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(237..>763)
                     /locus_tag="ACG8FM_002996"
     CDS             complement(237..>763)
                     /locus_tag="ACG8FM_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095567.1"
                     /translation="LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKNIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          261 a          102 c           85 g          315 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttatattt
      481 ttacctttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
      541 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa catctaaact attaaaaaca
      601 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
      661 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
      721 agctcagttt gtttatcatc ctcaataatt ttataaatca aac
//
LOCUS       DAZUMT010000251          651 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000251 DAZUMT010000000
VERSION     DAZUMT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.264"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>651
                     /locus_tag="ACG8FM_002997"
     CDS             <1..>651
                     /locus_tag="ACG8FM_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFS5095568.1"
                     /translation="KLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIV
                     MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLV
                     DMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVK
                     VQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEK"
BASE COUNT          239 a           74 c          130 g          208 t
ORIGIN      
        1 aaaaattatt ggcaaatgag atttggagta gtttagaagt cgaagaaagt aaacaagaac
       61 ttttgttgaa atggatactt tttcttgtaa agaagttcta tgtgaaaata ccagaaaccc
      121 attgtctgat agttatgcac ggtaaaataa cagcttctgc gatcgcaagg gaaacaaata
      181 agttgctgaa tacatatgtt tatgaggctt tcgatatgcc aattgaaggt gagactagtg
      241 acttaattaa cctaatcaac aatttttgta aaagtataga tacgacgaat ggactcattc
      301 tgctagttga tatgggatca ttggaacaaa tgtatgaaaa aattgaatcc aatgtaatag
      361 gtgatttggt aattttaaac aatgtttcta cagctttagc gattgaatgt gggattcaag
      421 tttgccaaaa aaagccaatt agtcattttt accagatgga ttttgattcg tttcaagtta
      481 aagttcagta ttataaagga ttatcacaaa agaaaaatgt aatagtatct tgtctctctg
      541 gtgaggggat ttctgaaaaa gttaaggata ttttaaaacg gtatctcgat aatcaagtgg
      601 agattttgat ttttgatttt aacgcattaa agaaaatagc aaaggaaaaa g
//
LOCUS       DAZUMT010000252          642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000252 DAZUMT010000000
VERSION     DAZUMT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.266"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            219..617
                     /gene="spxA"
                     /locus_tag="ACG8FM_002998"
     CDS             219..617
                     /gene="spxA"
                     /locus_tag="ACG8FM_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS5095569.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          229 a           95 c          122 g          196 t
ORIGIN      
        1 tgaattagat ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta
       61 ctgtatatta accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct
      121 atttaaaagt tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac
      181 aaaaaagtga ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc
      241 caagttgtac ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg
      301 ttgaacgtaa tattttttca gaaccattga acagttctga attaaaagcg attttacaaa
      361 tgacagaaga cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga
      421 acatggattt agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg
      481 gattgcttcg ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag
      541 atgaaattcg tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat
      601 tgatggctgg attatagaag gataatacga aacttggaaa ag
//
LOCUS       DAZUMT010000253          588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000253 DAZUMT010000000
VERSION     DAZUMT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.267"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG8FM_002999"
     tRNA            complement(65..150)
                     /locus_tag="ACG8FM_002999"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG8FM_003000"
     tRNA            complement(167..238)
                     /locus_tag="ACG8FM_003000"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG8FM_003001"
     tRNA            complement(250..322)
                     /locus_tag="ACG8FM_003001"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG8FM_003002"
     tRNA            complement(340..421)
                     /locus_tag="ACG8FM_003002"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG8FM_003003"
     tRNA            complement(429..501)
                     /locus_tag="ACG8FM_003003"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG8FM_003004"
     tRNA            complement(505..577)
                     /locus_tag="ACG8FM_003004"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZUMT010000254          583 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000254 DAZUMT010000000
VERSION     DAZUMT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.268"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>583)
                     /locus_tag="ACG8FM_003005"
     CDS             complement(38..>583)
                     /locus_tag="ACG8FM_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5095570.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          100 g          206 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gat
//
LOCUS       DAZUMT010000255          576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000255 DAZUMT010000000
VERSION     DAZUMT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.269"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..>576
                     /locus_tag="ACG8FM_003006"
     CDS             80..>576
                     /locus_tag="ACG8FM_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS5095571.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          217 a          106 c           93 g          160 t
ORIGIN      
        1 aaaaatccat acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca
       61 cagaaaagag gacttcccta tgaatgattt tactacagaa attgtgcaaa ctctagtcac
      121 taaaggcgat ttaaatgaat tattccgttc gcacttagaa aaagcgataa acacactcct
      181 acggactgaa ttaacggctt ttttagatta cgaaaaatat gatcgcactg gttttaattc
      241 aggtaattcg agaaacggtt cttactttcg atcaatcaaa accgaatatg gtgaattaac
      301 attggaaata cctagagatc gtaatggtga gtttaaacaa caaactttac cagcctacaa
      361 aagaacaaac gatacattgg aaaccactat tatccattta ttcgaaaaag gtgttacgat
      421 gtctgaaatt gctgatttga tcgaaaaaat gtacggtcat cactatactc cacaaaccat
      481 gtccaacatg actaaagttc tgactgaaga agtaaatgcc tttaaatcca gagccttaaa
      541 tgataagtat gtcgctattt ttatggacgc tactta
//
LOCUS       DAZUMT010000256          575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000256 DAZUMT010000000
VERSION     DAZUMT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.270"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..575
                     /locus_tag="ACG8FM_003007"
                     /pseudo
     CDS             258..575
                     /locus_tag="ACG8FM_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DAZUMT010000257          575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000257 DAZUMT010000000
VERSION     DAZUMT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.271"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          186 a           81 c           81 g          227 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct tttta
//
LOCUS       DAZUMT010000258          571 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000258 DAZUMT010000000
VERSION     DAZUMT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.272"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..>571
                     /locus_tag="ACG8FM_003008"
     CDS             125..>571
                     /locus_tag="ACG8FM_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS5095572.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMRGKRKPNGHQEKRGKQQYQRSIHDR"
BASE COUNT          220 a           99 c          106 g          146 t
ORIGIN      
        1 ccgcactatg ccattacatt tatatctacg cgaattgtaa aattaagtta gaaaaataaa
       61 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
      121 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
      181 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
      241 taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc accagtataa
      301 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
      361 cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag
      421 gtctgtttca tgcggtatga gaacacttta tcgtttattt tctaaaggaa tatttgatat
      481 tgacacacta ccgatgagag gtaaaagaaa acccaatggc catcaggaaa aacggggaaa
      541 acaacagtat cagcgctcaa tccatgatcg a
//
LOCUS       DAZUMT010000259          569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000259 DAZUMT010000000
VERSION     DAZUMT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.273"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>569
                     /locus_tag="ACG8FM_003009"
     rRNA            <1..>569
                     /locus_tag="ACG8FM_003009"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          131 c          173 g          126 t
ORIGIN      
        1 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc
       61 gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg
      121 attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg
      181 cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt
      241 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa
      301 gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc
      361 cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt
      421 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt cagttgggca
      481 ctctagcaag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg
      541 ccccttatga cctgggctac acacgtgct
//
LOCUS       DAZUMT010000260          534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000260 DAZUMT010000000
VERSION     DAZUMT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.275"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACG8FM_003010"
     CDS             <1..>534
                     /locus_tag="ACG8FM_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS5095573.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZUMT010000261          520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000261 DAZUMT010000000
VERSION     DAZUMT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.276"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>520)
                     /locus_tag="ACG8FM_003011"
     CDS             complement(37..>520)
                     /locus_tag="ACG8FM_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095574.1"
                     /translation="FFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLIN
                     HIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRN
                     NLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE
                     "
BASE COUNT          164 a           92 c           83 g          181 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa
//
LOCUS       DAZUMT010000262          468 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000262 DAZUMT010000000
VERSION     DAZUMT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.278"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          152 a           90 c           56 g          170 t
ORIGIN      
        1 ggcgaaagca caaaaaataa aaaaattatt acgttattaa aaaaacagat gaaagatcag
       61 gttttattag atctcattct ttcatcactt gaagggattt aatgaactac tttttcctct
      121 ttgtcgtttt tatatattcg tattccttca aacagtagga tagcctcttt caatgcttga
      181 tatttgaact gtccgttact taaaaattta gatgtgccgc cctcttccta gaacatattc
      241 ttttttactt tttcattaat tgacagacca atcgaacgaa actgtaaatt tcgataggat
      301 aatgattggt caaactcatt cattttcaag acatctcgat tcatattcaa gataggaatc
      361 tttttatcaa catcacttta ttcgtatttt cctctaccat aaaagcttca cgctccacaa
      421 aattgtttta tacactacta ttcagcccat tcaacgacca atgaattt
//
LOCUS       DAZUMT010000263          422 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000263 DAZUMT010000000
VERSION     DAZUMT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.279"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8FM_003012"
     CDS             complement(<1..132)
                     /locus_tag="ACG8FM_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5095575.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          144 a           82 c           83 g          113 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 ga
//
LOCUS       DAZUMT010000264          380 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000264 DAZUMT010000000
VERSION     DAZUMT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.281"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>380
                     /locus_tag="ACG8FM_003013"
     CDS             <1..>380
                     /locus_tag="ACG8FM_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS5095576.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHK"
BASE COUNT          133 a           71 c           62 g          114 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa
//
LOCUS       DAZUMT010000265          368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000265 DAZUMT010000000
VERSION     DAZUMT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.282"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG8FM_003014"
     CDS             complement(<1..272)
                     /locus_tag="ACG8FM_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095577.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAZUMT010000266          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000266 DAZUMT010000000
VERSION     DAZUMT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.285"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8FM_003015"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8FM_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095578.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZUMT010000267          302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000267 DAZUMT010000000
VERSION     DAZUMT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.286"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>302
                     /locus_tag="ACG8FM_003016"
     CDS             <1..>302
                     /locus_tag="ACG8FM_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS5095579.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          117 a           48 c           68 g           69 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 ag
//
LOCUS       DAZUMT010000268          293 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000268 DAZUMT010000000
VERSION     DAZUMT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.289"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>293
                     /locus_tag="ACG8FM_003017"
     CDS             <1..>293
                     /locus_tag="ACG8FM_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS5095580.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKK"
BASE COUNT          101 a           43 c           58 g           91 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aac
//
LOCUS       DAZUMT010000269          281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000269 DAZUMT010000000
VERSION     DAZUMT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.290"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..131
                     /locus_tag="ACG8FM_003018"
     CDS             <1..131
                     /locus_tag="ACG8FM_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095581.1"
                     /translation="RVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT           83 a           59 c           57 g           82 t
ORIGIN      
        1 aacgagtgat tcggattttt ccaaatatca attccatcac tcggataatt ggaacacttt
       61 tgatggaaaa agacactgag tggctggctt ctccacgaaa atatttagaa tttaattcga
      121 ataacattta gcaccatgaa cctgtgcgag tctcttcttt ctgaggagag acatcaagag
      181 tcgctgcgct actgctcctg acctctctcc tcagaaagaa aggcatggca gttaggttca
      241 caacgcaaaa cattgtgctt ttgatgaggt tgaactttta c
//
LOCUS       DAZUMT010000270          266 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000270 DAZUMT010000000
VERSION     DAZUMT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.291"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>266)
                     /locus_tag="ACG8FM_003019"
     CDS             complement(34..>266)
                     /locus_tag="ACG8FM_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5095582.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DAZUMT010000271          261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000271 DAZUMT010000000
VERSION     DAZUMT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.293"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>261
                     /locus_tag="ACG8FM_003020"
     CDS             <1..>261
                     /locus_tag="ACG8FM_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095583.1"
                     /translation="PGQAATITIGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPG
                     INATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNATTTT"
BASE COUNT           87 a           54 c           56 g           64 t
ORIGIN      
        1 tcctggccaa gcagcaacaa ttaccatagg aaccgtttct agtggttcaa ccgcttctgt
       61 tacgaatgta ggaacatcat ctgcagctag atttaatttt gttttaccaa agggagataa
      121 aggtgatcct ggaataaatg ccacaactac agctgtagct acaactacag caaatgggtt
      181 gatgtcgtcc acagataaaa ctaagttgga tggaattgca gctggagcac agaaaaatcc
      241 aggtaatgct acaactacga c
//
LOCUS       DAZUMT010000272          253 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000272 DAZUMT010000000
VERSION     DAZUMT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.294"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACG8FM_003021"
     CDS             <1..>253
                     /locus_tag="ACG8FM_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS5095584.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAZUMT010000273          252 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000273 DAZUMT010000000
VERSION     DAZUMT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.295"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..>252)
                     /locus_tag="ACG8FM_003022"
     CDS             complement(32..>252)
                     /locus_tag="ACG8FM_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS5095585.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAZUMT010000274          248 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000274 DAZUMT010000000
VERSION     DAZUMT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.denovo.296"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..>248)
                     /locus_tag="ACG8FM_003023"
     CDS             complement(44..>248)
                     /locus_tag="ACG8FM_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFS5095586.1"
                     /translation="KVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           63 a           62 c           49 g           74 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttg
//
LOCUS       DAZUMT010000275        14078 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.guided.076, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000275 DAZUMT010000000
VERSION     DAZUMT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.guided.076"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..654
                     /locus_tag="ACG8FM_003024"
     CDS             <1..654
                     /locus_tag="ACG8FM_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5095587.1"
                     /translation="FKAEHEAMLKQAQDEPAIDEIYHFDFLEAKELGLKHNSSFYSKH
                     MNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFILPHRGNEQPGYLTYE
                     EYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRPENIFLL
                     RERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(804..1745)
                     /locus_tag="ACG8FM_003025"
     CDS             complement(804..1745)
                     /locus_tag="ACG8FM_003025"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5095588.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1871..2992
                     /locus_tag="ACG8FM_003026"
     CDS             1871..2992
                     /locus_tag="ACG8FM_003026"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5095589.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3250..4752
                     /gene="eat(A)"
                     /locus_tag="ACG8FM_003027"
     CDS             3250..4752
                     /gene="eat(A)"
                     /locus_tag="ACG8FM_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5095590.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4819..5346
                     /locus_tag="ACG8FM_003028"
     CDS             4819..5346
                     /locus_tag="ACG8FM_003028"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5095591.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5557..6618
                     /locus_tag="ACG8FM_003029"
     CDS             5557..6618
                     /locus_tag="ACG8FM_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5095592.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6621..7517
                     /gene="murQ"
                     /locus_tag="ACG8FM_003030"
     CDS             6621..7517
                     /gene="murQ"
                     /locus_tag="ACG8FM_003030"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5095593.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7531..9513
                     /locus_tag="ACG8FM_003031"
     CDS             7531..9513
                     /locus_tag="ACG8FM_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5095594.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9578..10024)
                     /gene="nrdI"
                     /locus_tag="ACG8FM_003032"
     CDS             complement(9578..10024)
                     /gene="nrdI"
                     /locus_tag="ACG8FM_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5095595.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10235..11659
                     /locus_tag="ACG8FM_003033"
     CDS             10235..11659
                     /locus_tag="ACG8FM_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5095596.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11840..12613)
                     /locus_tag="ACG8FM_003034"
     CDS             complement(11840..12613)
                     /locus_tag="ACG8FM_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5095597.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12610..13515)
                     /locus_tag="ACG8FM_003035"
     CDS             complement(12610..13515)
                     /locus_tag="ACG8FM_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095598.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13669..13989)
                     /locus_tag="ACG8FM_003036"
     CDS             complement(13669..13989)
                     /locus_tag="ACG8FM_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS5095599.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4490 a         2521 c         2997 g         4070 t
ORIGIN      
        1 ttcaaagcag aacacgaggc gatgctgaag caggcacaag atgaaccggc aatcgatgag
       61 atttatcatt ttgatttttt ggaagcaaaa gaacttgggc tgaaacataa tagttcattt
      121 tattcgaaac atatgaattt ttcttctagc gaaccaaaga cggatctatt ttttggtgca
      181 acgaataatg ggagaaaaga acgagcagaa agctataagc aggaatttat aaaaagagat
      241 gttacgacca actatttcat tcttccacat cgagggaatg aacaacctgg atatcttact
      301 tatgaagagt acctgaaaaa aacaggggaa tcgcgcggga ttctggaact gttgcgggag
      361 ggtcagcaag gagtgacttt gcggacattt gaatccatgt attttcaaaa gaaactagtg
      421 acggataatc aagcgattgc tcattatcat ttttatcgtc ctgaaaatat ttttttgctt
      481 cgagaacgtg atttagatga attaccggaa tttctgcata caccatttaa gccaatccta
      541 tcagagctat tggaatttta tgatgcagaa aattgggcaa aacgatttat cactaacgat
      601 tccactattt atgagcaata cgaatatgac ctctcctctg ttgaaaacaa ataaaaaacc
      661 tacattttga agagattatg tactgaaaca aacaagcaca attcttatgg catcatgaga
      721 gttgtgtttt ctttttgatc ttttattggg cttaaaaaaa gagaaccagc tgctgattca
      781 gctgattctc atcagagagc cttttattct gcgccaagga ttccttcttt tgcatggact
      841 aattgggtca aaaaggcaca atatggtgta gcaatatcat atttttgtcc ttttcgccaa
      901 atggcaccat tgatgtagtc gatttcagtc aaacgatggt ttttgatcag gtcttggtac
      961 atagatgggt aatgcaaccc gatgttgtca ggatcataag tagaagcaat atgttggtac
     1021 acttcttctt gatctagtat gattccttct tttgctgcga tagaagcaaa ttcagatacg
     1081 attgttcgaa ccatgctctc tgccgctttt tgcgcgccta gttcagcaat attacaatcc
     1141 aaaatcgtgc ataaaccatt caatgttcca tttacacaag cttttcgcca aatggaataa
     1201 cgaacgttat ggctatagat cggatttaaa tttgcttctt gaaagacttc tacaactttt
     1261 tttgtaaatg cttcgccttc tggttccaag ttctctagtt cgatttctcc atcacctaac
     1321 aatgtgactt ttccgggacc agctagacca gctgtccaca tcgtgatccc aaataagata
     1381 tttttctttg gcacatattt ttccagtaca tcttcatgac ctaatccatt tagtaaacac
     1441 aaaacataag tattgtcagt aataatggat tggatcgaac aaaacatatc atctaattga
     1501 ttggcttttg ttaatgctac gatcaagtcc acgtgttcgt tgctttcgat gatttcttct
     1561 ggtgaataga ttggcagttt tgcgaccact tctttaccgt taaagtcagc aatcaaccca
     1621 tttgtacgaa tggcttcaat gtgagcaggc caacgatcga tcaagaggac ctcatttccg
     1681 ctttgatgta gcatcaatcc gattcggctg cccattgctc cggccccagc tattgcgatt
     1741 ttcatgtttt ttctctcctt tattcaatga gttccctttt agtatataga aaaatggaag
     1801 aaaatagcat tgttttgttt gaaaaaaatg aaaaaaagtg tcttacttaa gaagaaagtg
     1861 agggatacag atggatattt tggcggcaat tgattcattt aaaggaagtg cttcttcatt
     1921 tgaactaaat caggcagttc taaatggctg ggaagatcgt aaaggaagaa aaatcaatgt
     1981 gccgattgca gatggcggag aaaatactgc agaagcaatc tattacgcgc tcaggggaac
     2041 atggcgtacc attgatggga tggatctctt attccgtcag cgaaaaatca gctatttatt
     2101 gacaacttgg caaggaaaaa acaccgcagt catcgaatct gcacaagcat tggggttaga
     2161 tctacttgat aaaccaacgg atcaaactat ccgacttgct tccagttatg ccttaggaga
     2221 acttattaaa gatgcactgc ttcaagaagt agaacaaatt attgtcactt taggaggaag
     2281 cgggtgtagc gatggtggtc taggattact ccaatccttg ggtgcttctt tagaagggta
     2341 cacagaagga aatcctttat tatctactaa aaaacttcat tttgatcgaa gaaacgttct
     2401 tgtttctaaa gtaaaattga ttatcgctgc agatgttacc aatccatata caggtcaaca
     2461 aggagctgct tggcaatttg gaagacaaaa aggaggaata gatacgacgc tagctttctt
     2521 agataaacaa gcaggaaaga ttgctcagca aataaaagat ttctgggagg tggatttaga
     2581 tgatattcca ggaagtggag cagcaggcgg tattggtgga gccctttttt tgttgggagc
     2641 gaagatggtt tctggtttcg atttagtcag ccaaatcact ggattggaaa aaaagatcga
     2701 acaagcagat gtcatcatta caggagaagg gcggatcgat cagcaaacga tccacggaaa
     2761 agttccatac ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg
     2821 cggaagtaga gaccaagagc tagaaggact ttctgatttt ctcccaattg tcttaagtat
     2881 tcatttgggt ccaataggtt tagaagaagc aatggaacac aggcaaacat tagaaaaaac
     2941 aaaaatctta ggacagacac ttagcagatt tttcaccatc ctcccgaagt agttgacaaa
     3001 atcattttct ttatgctatc gtaatttcaa atataggaca aagtgaggga tccgtgcatt
     3061 gaaaaattaa gtcgattgat gaaagctgca ttcagactgt accttgtgga gacaaggaag
     3121 cagtgtgcag attttcggga ttggcttggt tttttcgatg tatgggtctt tttgtgtgta
     3181 tccaaccctt gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg
     3241 tgatggaaaa tgtctaaaat cgaaataaaa aatctgacat tcggctacga cagccaaggc
     3301 acattattat ttgaacaagc aaatctaaat tttgacacac aatggaaact aggacttatc
     3361 ggacgaaacg gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa actaccttat
     3421 caagggcaag taatccatca gcaagaattt gcctatttcc cgcaacagac aaaagataaa
     3481 gaacgtttaa cctattacgt gttaaatgat attacggatt ttgagatatg ggaaatcgaa
     3541 agagagctcc aattgatgca aacagatcct gaaatcttat ggagagaatt cagcacacta
     3601 tcggggggag agaagacaaa agtcctactg gcacttttat ttgtggatga cactcatttc
     3661 ccgttaatcg atgaaccaac gaatcatttg gatatctctg gtagaaaaca agtagcggct
     3721 tatttgaaaa agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg atttatcgat
     3781 gaagtagtgg accatgtttt agcaatcgaa aaaagtcaac tggaacttta tcaagggaat
     3841 ttctctatct atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa
     3901 aaattgaaaa aagaagtcag taggctaaag aaaacagcag ctgaaaaagc cgaatggtct
     3961 cgttcccgag aaggagataa aacaaagaaa caagtcggat tcatcgatac tgaatctaga
     4021 cgagtgaata aaggagcagt gggtgctgat gctgcacgga cgatgaaacg atccaaagca
     4081 atcgtgaatc ggatggagac ccagatcagc gagaaagaaa aactattaaa agatatcgaa
     4141 tatatcgatt ctttgacgat gaatagccaa gcgtctcacc ataagcgact tttaagcgta
     4201 gaagatcttc aattagggta tgaaaatctg ttattcgagc caattcattt tacaatcgag
     4261 cctcatcagc gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc cattatccat
     4321 tatcttctgg gggcattcaa cggcaaggtt ataggagaaa aataccagcc aaaacatctg
     4381 agcattagtt atgcaagcca aaattatgaa gacaatcgag gaacgttggc ggaatttgca
     4441 gagaaaaacc aagtagacta ccaagcattt ttgaacaacc tccgaaagct tgggatggaa
     4501 agagatgttt ttcataacaa gatcgagcag atgagtatgg gccaacggaa aaaagtggaa
     4561 ttggctaaat ctttatcaca gccagctgaa ctatatacat gggatgaacc attgaattat
     4621 ttggatgtct tcaatcaaga acaattagaa caactgatct tgaacgtgaa acctgccatg
     4681 ttactagtgg aacatgatca aaccttcctg gataaagtat ctactgagat tatttctctt
     4741 gagagaatct aacccttgta atctcaagaa caacaaggta tggttaaggg gaactttgcc
     4801 aacgaaggag agaatcacat gctttatgta acgagacacg gagaaacaac ttggaacgca
     4861 caaggattag tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca
     4921 cagaaactag cagaaaaggt agtagatttg cctgttccaa tcacgaaaat catccattcc
     4981 cctcttcaac gagcaagaga tacagcacaa gcagttgctg atcgtctcag cttgccgctg
     5041 accgttgatg aacgattggt agaaatggat tttggggatt atgatggact gccaagcaaa
     5101 gatgagaact ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa tggggagtca
     5161 gtcttagatg tgtatgcacg gatcgttcct ttattgaaag aatgcatcga agacgaagaa
     5221 aatgtctatc tgttagtttg ccataatgcc ttgataagag tgatcaatgc ttattttcat
     5281 ccgatgccta atgaggggtt ctttacattc atggtagata atacagaatt ggtttcttat
     5341 gagtaattat gtttttgtgt tatttaaata aaatatttca aggcactgct tttgcttagc
     5401 agtgcctttt ttaggttatg acaattcttt tgataatata aactcaacct ttagaaatga
     5461 aatttaatta ttttaataaa agtattgaaa ttttgtttct ttagatatat actctagtca
     5521 tagaaatgaa gacggataca gaaaaagagg gataatatgt ttggcttttc aatatttatg
     5581 aacgattctt tgtcagaaga gaagaaacag tatatccgcc aaatggcatc caatggcttt
     5641 gtcggtattt tcacttccat gcatatacca gaagatgatg tttcggcata tcaaaagcga
     5701 ttgatcgaac taggaagcga agcgaaaaag aataaactag aattgatggt cgatatttca
     5761 ggagaggcat tggaacgagc gggtttttca ttagaagatc taacgccttt gaaagcaatt
     5821 ggtgtaacag gtttaagaat ggattatcat atttccaatc agcagattgc cgactggtca
     5881 catcaattga aaatcagttt gaatgctagc acgatcacgc ctaaagatat cgatgaattg
     5941 aaagaagcag aagctgattt ctcacagatt gaagcttggc ataattatta tccacgtccc
     6001 gaaacagggc tggacaaaga atggtatcag aaaaaaaatc aatggctcaa aattcaaggt
     6061 ctccttgtcc aaggttttgt accaggagat acagagttga gaggcccact atatcaagga
     6121 ttgccaactt tggaagaaca tcgagggatc catcctcttg caggcgctct agatctatta
     6181 gcaagtaaca cggatattgt ttacatcgga gatgcaggtc tgtcagaaaa cgttcaggag
     6241 caatttgctt ctttccaaaa agaacagaca gtcttgctcc atacggaacc tgtagatgaa
     6301 gaattttacg aatatatatt agggaagcat acgaatcggc aagacgatgc aagagatgtc
     6361 atacgaagcg tagatgcacg ttttcgagag attccgccta tcccagcaag aaatacagct
     6421 acgcgtatga aagggtctat tactttagat aacgaaaagt atttgcgcta tatgggtgag
     6481 atccaattaa ccaaatatga tttgccagcg gatgaaaaag tgaatgtagt agctaaagtt
     6541 atcaaagaag agctaccatt gatcaaccaa atcaaagctg gtatgaacta tcaatttata
     6601 agaaaagaag gaagataaaa atgagcaaaa tcaatttaga gaacctaaca acggaacgca
     6661 gaaaccaaga aacttttgga ttagatgaga tgagtatttc tcaagccgtt caactaatga
     6721 acaaagaaga tcagaaagtg gccgctgcgg ttgaaaaaga actgacaagt atcgaaccag
     6781 tgattgctaa cacgattgag tcctttaaaa aaggtggacg tctgatttat atgggagccg
     6841 gaacaagtgg aagactaggt gtgttagatg ctgctgaatg cgtaccgaca ttcggtgtag
     6901 aaccggaaat ggtcataggt ctaattgctg gtggagaaga agcaatgact gtagctgtcg
     6961 aaggggccga agattcttat gagctagggc aagaagatct aatcaagctg aatttgacaa
     7021 aaaatgatat agttattggt atcgcagcta gcggtcgtac accatatgtg atcggtggat
     7081 tagattacgc aaaatcgatc ggtgctgtca caggaacgat ttcttgcaat aaacaagccg
     7141 aaatcagtaa acacgcggat ttcccaatcg aagtcgatgc aggtccagaa tttttgacag
     7201 gctctactcg attgaagtca ggaacagcac aaaaacttat cttgaatatg atttctacta
     7261 tcagtatgat tggtataggt aaagtattca ataacttgat ggtcgatgtc aaaccaacaa
     7321 atgaaaaact aatcgaacgc tcaaaacgaa tcatcatgca agcgacagaa gctgattacg
     7381 aaacagctga aaagtacttt gaagaagcag atcaaaatgt taagctggct atcgtcatga
     7441 ttttgacaaa tagtgataaa gaggaagctc aagcaaaatt agctgaagca aacggatttg
     7501 taaaagggac cttatgaggg ggaaaagacc atggcaaaaa acgaactgac tttggaagaa
     7561 ctggcaaaac aaatttataa aggggcaggc ggcatgggca atgtagaaag cattgtccac
     7621 tgcatgactc gtgtgcgtat gaagatcatc aatgatgatc ttgtaaatgt ctctgaatta
     7681 aaagcgattc caggagtgct tggtgtagta gaagatgaac agcttcaagt catcatcgga
     7741 cctgggaaag tgaacaaagt agcacagcaa atggttgatt tggcaggcgt gaaattagga
     7801 gaaaaacttt cttctgtaag tacagctaca gcaaattcat cagcatctgg aaaagaccgt
     7861 gtaaacgaac gggcacaaga aatgaaagcg gcacaaaaat cgaaacaaaa accatctaaa
     7921 atcaaatcgg tattaaaaga catttctaat atctttgtgc caatgattcc tgcatttgtc
     7981 ggtactggga tcgtggcagg gattgccgca gttttagcaa acttagtcac agccggaaca
     8041 cttgatgcag caacatggca acaatatatc gatgtgatga acatcttgaa aaacgggatg
     8101 tttagctacc tagttatcta tacaggtatc aatgctgcac aagtttttgg tgcaacacca
     8161 actcttggtg gagttatcgg ggccgttgtg atgctgacag ggatgaatcc ggaagcacca
     8221 ctgaaaaact tatttactgg tgaagctctt gcagctggac aaggtgggat cctaggtgtc
     8281 atcttcgctg tttggctatt gtcaatcgtt gagaaaaaac tgcataaagt agtacctgat
     8341 gcggtagata tcatcgttac accaactctt tcattgattg ccatcggtct gattgaaatt
     8401 ttcttcatca tgcctttagc aggtttcatt tctgatggca tggtcggtgg gatcaactgg
     8461 gtacttggcg ttggtggagc atttgccggc ttcgttctag gcacattatt cttgccgatg
     8521 gttatgttcg gactgcatca aattttgact ccaatccatg tgcaaatgat tgatgaaact
     8581 ggtcgtacgc tattgcttcc aattcttgcg atggctggtg caggacaagt gggtgcagca
     8641 ttggcattat ggattcgctg caaaaaagat aaagaattaa cagaaatgat caaaggagct
     8701 ttaccagtcg ggatcctagg aatcggcgaa ccattgattt acggagtaac attacctcta
     8761 ggacgcccat ttattactgc ttgtatcggt ggaggtatcg gcggggcagt gatcggaatg
     8821 atcggaaatg tcggggctat cgctattgga ccttcaggag ccgctttgat cccattgatc
     8881 tctgacggta aatggttagg ctacatcctt ggattattgg cagcttatgc tggtggcttc
     8941 gttgctacat tcttcttcgg tattcctaaa gaccgtttag aaaaagaaga attagcgact
     9001 gaaacagcca cagtaaatga agtgcctgaa aaagtagtac ctcaaacttc agctgaaagt
     9061 gtgactatcc tctcatcagt tgctgacgga acagttgttt ctttagataa agcaagtgat
     9121 ccagtttttg cacaaaagat gatgggtgaa ggctactttg tagaaccaga aaacggacat
     9181 atctattctc cagttactgg ggtagtcagc tctgtcttcc caacgaaaca cgctattggg
     9241 atcacaacgc caaatggttt agaaatcttg cttcatatgg gaatcaacac agttgactta
     9301 ggcggtacgc catttgatgt gaaagtaaca gaagggcagc aagtgacacc tgatacgctt
     9361 gtagcagaag ctgacttaga agcgatcaaa gcagcaggca aagaaacatc catgatcgtc
     9421 ttggtaacca atatggatcg tgtaaaaaac tttgtcttgg aaaaaacagg taaagcaaaa
     9481 gcaaaagcac ctgtgatgga tgtcaaagct taatatgata gaaacaatag aaacgaaaga
     9541 agaaactgaa atctattttt cagcttcttc ttttttatta tttattgttt tctgctaaaa
     9601 tctgataaat tcgtgtcaca tcagatggtg ttccccgtaa ttcataatct gccaagaaag
     9661 gaaagccaaa tgtttgtgca tattgtttgg ctgttagaca atactgatta ttgaagtttt
     9721 tatttccgct accgactaca ccaagacaaa gtttatgatt gtcttcatgt tccagatatt
     9781 cacgcatcgt ttcggtcaag atttctgtat caccgttatc taaaccattt ccgccgtcta
     9841 aataagtagg aacgaaagta aaaaatggtt ctgtttcttt cgtaaaatca ctattttcat
     9901 ggatttcttt taaagtaact tcaggattga caggatcttc tgcatgcatt ttttcagcat
     9961 attctgctag atgtttggca aaggcacgcg tatttcctga aatcgatata tataaaatgt
    10021 tcatggatca tcctccaatt ctttcctttt agtatagcat cttttttaag ttttttggtg
    10081 gtgtaatttg gaaagggact aggcgctttg cttgtataat gagaaaaaag ccggtagaat
    10141 tatatttcta ttaaagtttt tattaattaa gtaaaaataa aaaaggtttg ttatactttt
    10201 tgataaaagc ggttttattt tggaggatgc ttacatggat acagagcaac agaaaaaagg
    10261 aattggttta attccacttg ctgccttagt aattggttca gcgattggag gtggcgtatt
    10321 tggtattatg acggacatct ccggatcggc tggcggccca gctattttca gttgggtact
    10381 cgtaggaaca gcaatgttga tgctctcctt gtcgatcaat aatatcaata aaaaacgtcc
    10441 agatttaaaa ggcgggatct ttagttacgc agaagaaggt tttggccgtt tcgctggttt
    10501 tatcagcggt tggggttact ggctcacatg ctggttaggc aacgtagctt ttgcaacatt
    10561 gttgatgagc gcattaggat atttcttccc agtatttgaa ggtgggcaaa atctggcatc
    10621 gatcgtgatg agtacagtct ttttatgggg atatgcatgg ctagtgaatc ggggagtcga
    10681 aaatgctagt ttcgtcaatg ctatcgtcac attctgtaaa ttagtgccat tatttttatt
    10741 tattgtagta gctatcagca cgttttcgat ccaacaattc attgataact tttctagcga
    10801 cattatattg aatgcgaatg gacagccgat cccgttttcc agtcaagtat tgagttgcat
    10861 catggtgatg ctttgggtat ttgttggtat tgaaggagcc tctgtcgtag gttcacgagc
    10921 aaaaaaacaa tcagatgttg ggaaagcaac gattctaggg atattcggct tgattattat
    10981 ttacgtcttg gtttccatgc ttccatatgg gacaatgacg atgaatcagc tgcaaacgat
    11041 caaggcgcag cctgctatgg gctatgtgtt tgaaatgatg gttggtaaat ggggagcagt
    11101 cgtgatcaac ggcggtttga tcatttcttt agttggtgtt tggctctcat ggacgatctt
    11161 accgattgaa acaatgggaa acatggctga agacggactt ttgcctaaag catggggaaa
    11221 actcaacaaa aatggggcac caacttatgc tatcgtattg acgacgttat gtacgaatgt
    11281 ctttttactt tcattattat tcactgacag tgcttataac tttgcttaca cacttggtac
    11341 agcagctatt ttcttcacgt ggcttttctt aggattgtac caaatgaaac tttcttatca
    11401 gcggaaagaa tgggtccaat ttttagtagg gcttttagca agtgcctttc aagtatgggc
    11461 tatgctagtc gtagccttta atgaagtaat gcttgttctt gttcttttct tgccaggcat
    11521 ttatttttac ctcaaagcac gtaaggagca aaacgagcct gtacaacata tccacttaga
    11581 taagatttta cttataggtg tgacgatggt cggagtgatt gcaatggttt tatttgccac
    11641 tgggatgctt cacgtataga agtataaaag aagtataaag agaggttgtg agcctgtcgt
    11701 ttattcaaaa acatactagg aaaggagctg tgacacaaat gttgtcacag ctcctttttc
    11761 agaataaacg gtgtttaaga gacagccaat cggcaataag gctgaacgtc tctttcacag
    11821 cctctttttt gatgagtcat catacatttt ttcgcttaaa tacgagatat cctaatgcta
    11881 agaacaaaac gatgtaagct aaattaccga atagcatttg attagtagaa agatgagtca
    11941 attgcttcat gatctcttcg tttccaattt ggttttgcag attcaacata ctgatcggat
    12001 tccatttgat ccactcccat ttttggatga gtgcaataag gataccagat atgattgacg
    12061 aagcaaaata aaagacgatt cctgctgaga tggatgcgcc ggaactattg ataaagcaag
    12121 caatcatcaa cactaaactt aaaatcagcc ataaaccgat ataactaccc aaagtataag
    12181 tgaacaagct ttggatcagt gtttgaccag tagaaacctt ctccgtgaag gaaatcgaag
    12241 ggaaaagaat cagtttcatc aacacagtga caataatagc taaaaggtat aaataaacag
    12301 aataaataac taatgtgatc catttgctta ctaatatttc tcctcgagaa tactttcgat
    12361 aaaggagatt tttcaatgta ccatattgtg cttccatcga aatgatcgtg cttgcagctg
    12421 caatcatgat aaagacaatc caagatgtgg cactaaaaag ctgagcagtc atttcaactg
    12481 gatcaatgat cgtcgtgtat tttttggcta gaagtgctgt tcctatgagc aaaacaacta
    12541 atagaacaga taaaacagca gttgactttt tcttgatcaa tttaaataat tcttgtttta
    12601 caagcaatgt catttttctt cttccccctt tactgaatcc agaatttcca atagagaaga
    12661 ttccaaatca ttcgtcagat gctggacgtc tgtcacatgg atattttgtg cagacaatag
    12721 ctggatcacg gttcctaata attcttcgtt ttcttttaaa agcttgattt tatcaggatc
    12781 ttccaaagta tatccagctt ctgtcaagat cgttcgagca gctagatcat ttgttgtaga
    12841 gataacgatg aaatcttgcg ctgtttggtt caattcagcc attgtacatt gtttgatgat
    12901 ctttccttga ttgatcagga tcagctcatc gaccattttt tcaagctcgc tgagtaagtg
    12961 actggagatc aagaaagagg tgccctgagt agccaagtcg gtaatgattt ttcgcacatc
    13021 tcttgttgct tgtggatcca aaccgttcat cggctcatct aaaataacga tagacggatg
    13081 gttcagtaag gcaagagcaa ttcccaattt ttgtttcatt cccaacgagt aggatttcgc
    13141 ttttttgtga atgtactttt ccatcttgag ctgattcacg atagtgtcga tagcagatac
    13201 atcgttggtt tcagagaata acttcagatg atcatagcct gttaagaatg gatagatacc
    13261 cgggtattcg atcaatgctc ctacttgttc caacccttta tgactagagg gagaaatctc
    13321 attttcacca atacagattg ttccatctga gtaatggatc agtcctaaaa tagctttcat
    13381 gatcgttgtt tttccagcgc cattaggacc tactaatcca acgattttgc caggggataa
    13441 gctaaaactg acatcgctga gggcttgttt gtgaccaaag ttcttggata attcttggac
    13501 cgaaagactt ttcaaaaata ttcactcctt ttctatagat agtacaagca tacacctgat
    13561 gagtggagaa gtcgctaggt cattggactt atttcttatt ttttcataaa aaaattgaat
    13621 attaaataaa aaggcttatt ttttctgatc agtacttatt ccaagtgtct agtggccgct
    13681 agtaatcttg atgccaatga tactgatgag tagcatcccc acaaagaacc aagtggaaag
    13741 attcagctta tcatgaaaca agaccacacc aattatgacc gaaccaactg cgccaatccc
    13801 tgtccagata ggataagaga gactcatcgg caaacttttg attgcctttg acaaaaagta
    13861 aaaactagca atcatgccaa tcactgtgta aaaagaataa ttgattttag aaaaaccatc
    13921 actccatttc atcatcgttg accagaatac ttccaaaatt cctgctatga ttaattccat
    13981 ccatgccata cttacattcc tccttaacta taaaaaaata cacaaaaaga ggaaaatcat
    14041 cccttcaaag acctaatgga atgatcatcc tctttatc
//
LOCUS       DAZUMT010000276        10087 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.guided.102, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000276 DAZUMT010000000
VERSION     DAZUMT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.guided.102"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..523
                     /locus_tag="ACG8FM_003037"
     CDS             353..523
                     /locus_tag="ACG8FM_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095600.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            537..1139
                     /locus_tag="ACG8FM_003038"
     CDS             537..1139
                     /locus_tag="ACG8FM_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5095601.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="ACG8FM_003039"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="ACG8FM_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2143..2640
                     /locus_tag="ACG8FM_003040"
     CDS             2143..2640
                     /locus_tag="ACG8FM_003040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFS5095602.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2641..3057
                     /locus_tag="ACG8FM_003041"
     CDS             2641..3057
                     /locus_tag="ACG8FM_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFS5095603.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="ACG8FM_003042"
     CDS             3077..4621
                     /locus_tag="ACG8FM_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5095604.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="ACG8FM_003043"
     CDS             4764..4988
                     /locus_tag="ACG8FM_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095605.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="ACG8FM_003044"
     CDS             5003..5872
                     /locus_tag="ACG8FM_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5095606.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="ACG8FM_003045"
     CDS             5853..6587
                     /locus_tag="ACG8FM_003045"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5095607.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="ACG8FM_003046"
     CDS             6620..7528
                     /locus_tag="ACG8FM_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS5095608.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="ACG8FM_003047"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="ACG8FM_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS5095609.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="ACG8FM_003048"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="ACG8FM_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS5095610.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9230..9361
                     /locus_tag="ACG8FM_003049"
                     /pseudo
     CDS             9230..9361
                     /locus_tag="ACG8FM_003049"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9437..9930
                     /locus_tag="ACG8FM_003050"
                     /pseudo
     CDS             9437..9930
                     /locus_tag="ACG8FM_003050"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3548 a         1572 c         2207 g         2760 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DAZUMT010000277         8654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.guided.118, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000277 DAZUMT010000000
VERSION     DAZUMT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.guided.118"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(206..577)
                     /locus_tag="ACG8FM_003051"
     CDS             complement(206..577)
                     /locus_tag="ACG8FM_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5095611.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            722..907
                     /locus_tag="ACG8FM_003052"
     CDS             722..907
                     /locus_tag="ACG8FM_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5095612.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1065..1232
                     /locus_tag="ACG8FM_003053"
     CDS             1065..1232
                     /locus_tag="ACG8FM_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095613.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1850..2509
                     /gene="vanR-B"
                     /locus_tag="ACG8FM_003054"
     CDS             1850..2509
                     /gene="vanR-B"
                     /locus_tag="ACG8FM_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS5095614.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2512..3855
                     /gene="vanS-B"
                     /locus_tag="ACG8FM_003055"
     CDS             2512..3855
                     /gene="vanS-B"
                     /locus_tag="ACG8FM_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS5095615.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            4026..4832
                     /gene="vanY"
                     /locus_tag="ACG8FM_003056"
     CDS             4026..4832
                     /gene="vanY"
                     /locus_tag="ACG8FM_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5095616.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4850..5677
                     /gene="vanW-B"
                     /locus_tag="ACG8FM_003057"
     CDS             4850..5677
                     /gene="vanW-B"
                     /locus_tag="ACG8FM_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS5095617.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            5674..6645
                     /locus_tag="ACG8FM_003058"
     CDS             5674..6645
                     /locus_tag="ACG8FM_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS5095618.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            6638..7666
                     /gene="vanB"
                     /locus_tag="ACG8FM_003059"
     CDS             6638..7666
                     /gene="vanB"
                     /locus_tag="ACG8FM_003059"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS5095619.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            7672..8280
                     /locus_tag="ACG8FM_003060"
     CDS             7672..8280
                     /locus_tag="ACG8FM_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS5095620.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(8346..8519)
                     /locus_tag="ACG8FM_003061"
     CDS             complement(8346..8519)
                     /locus_tag="ACG8FM_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095621.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         2452 a         1821 c         2230 g         2151 t
ORIGIN      
        1 cggacgcggc ccggcaggga cggggcaggc cgtttttccg ggagtggcta cactccctat
       61 gcttgctacg ttactgtttc tccactaaca agacgtttcc aaagccccaa actacccgca
      121 aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa gctacgctgt gcagctcccg
      181 ctaaaagtaa aacagaggat gccagttaca catcctccgt ttcattcttc ttcggctttt
      241 caattagtat ggcgctgtca atcgcgccct cgatgatcgg caggtattct tcggggcaga
      301 gtttcagctt gtggctgacc ctttgccgca gttcgctttc ctcccgcatg atctccgggt
      361 taaagtagcg ttccacagga agcccgcagg ctttaataag ctgtatgaca accggcagac
      421 tgggaatggc cccgtcgttt tcgatgttgg caagatagcg ccagtcaata tttattttct
      481 ctgccattgc tttgcgcgaa acgcgctttg cttgccgggc cgctttcaca tccgcgccga
      541 aagtttcaaa gccgggacaa tcttcaatat tcgccatatc gcacctccca cttacattgt
      601 atatttcata ctctgcccgt ggaatgttgg tatatgcacc tttcttaaag tttatatttc
      661 acattttgtt tcttttattc ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac
      721 tatggactac atgacgttga aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat
      781 aaactactat tgtgctgcgg gacgcatccc cggcgctgta aagatggcga cgatctggct
      841 cattcccaaa actgcggaaa agccgattga caggcgtaca aagaaaggaa aggcggttac
      901 tgaatagtat aatgaaaccg gcgcattttg gatatggttt tgaagagaga gaaagcatgg
      961 tattttgtca cggaaaaact ataagatgat agatggcagg ctgctccaga ccgataaaaa
     1021 gtattctcat ctgaaacaga aagaaaagat ttacacatgg atgtatgaag aagcgaaact
     1081 ttatcatgac agaaatggaa aatgcccgaa acaaagacga ggaagctgct gctgtatcag
     1141 ccgtatatgg tagaattgaa aaggccggca tctggattct ttatggtgaa gtctccagac
     1201 attggataac tgctggtcta atgcgccttt gagtatgggg tgattgattt taataccggg
     1261 gaggtacggc tttttgagga aaatattctg ggatttacat ttggcgatta ttacatttcc
     1321 tgtaactgca aggacacgga tgatgaagat atgaggaata tagacttgct gattttctac
     1381 tgtcttgaac ggtattagga ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg
     1441 gagtacacac aggccctgcc tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag
     1501 aggccgcgca ttttgctgag gcttggggtg tccccaacaa gcaaaacgag aacgccgcag
     1561 gcgggcaagt ggtggatcag aatatccgta aaggatattc tgatccattg cttgccagta
     1621 ttatcgggat gttttgacaa ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta
     1681 attattatta tcttgattta attcgtttaa accaataata tagatacata caccatgaaa
     1741 agaagcgagt atcccaatga cggatgatta aaatccgaaa agaaatcgct ttgaaactac
     1801 agggaaacta cagactgtta tgttatcttc ttaaaatgga gggattttta tgtcgatacg
     1861 aattctactt gtcgaggatg atgatcatat ctgtaataca gtaagggcgt ttctggctga
     1921 ggcaggatat caggtggatg cctgcacaga tggaaatgag gcatacacca agttttacga
     1981 aaacacttat caactggtta ttcttgatat tatgctgccc ggtatgaacg ggcatgaact
     2041 tttgcgtgaa tttcgtgcga aaaatgatac tcccattctg atgatgacag ccctgtcgga
     2101 tgacgaaaac caaatccggg cgtttgatgc agaggcagac gactatgtaa caaagccatt
     2161 caagatgcag attttactaa agcgggtgga agccctgctg cggcgcagcg gtgcgctggc
     2221 aaaggaaatc cgtgtcggca ggctgacact tctgccggag gatttcacgg tactttgtga
     2281 cggtacagag ctgcccctga cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa
     2341 caaaggcaga accttaacgc atgaaatcat tttgtcacgt atatggggat atgactttga
     2401 gggtgatggc agcacagtcc acactcatat caaaaatttg cgggccaagc tgccggaaaa
     2461 tatcatcaaa accattcgcg gtgtaggata ccgattggag gaatcataat aatggaaaga
     2521 aaagggattt tcattaaggt tttttcctat acgatcattg ccctgctact gcttgtcggt
     2581 gtaacagcaa cactgtttgc acagcaattt gtgtcttatt tcagagtgat ggaattacag
     2641 caaacagtaa aatcctatca gccattggtg gaactgattc agaatagcga taggcttgat
     2701 attcaagagg tggcagggct gtttcactac aataaccaat cctttgagtt ttatattgaa
     2761 gataaagagg gaagcgtact ctatgccaca ccaaatgcca atacatcaaa tagttttaga
     2821 cccgactttc tttatgtggt acatagagat gataatattt cgattgttgc tcaaagcaag
     2881 gcaggtgtgg gattgcttta tcaagggctg acaattcggg gaattgttat gattgcgata
     2941 atggttgtat tcagcctttt atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc
     3001 aaagccttag cggacagtgc gaataaaatg gcaaacctga aagatgtacc gccgccgctg
     3061 gagcgaaagg atgagcttgg cgcactggct catgatatgc attccatgta tgtcaggctg
     3121 aaagaaacca tcgcaaggct ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca
     3181 cagcgatatt tctttgcggc agcttctcat gagctaaaaa cgcccatcgc ggctacaagc
     3241 gttctgttgg agggaatgct tgaaaatatc ggtgactaca aagatcattc taagtatctg
     3301 cgcgaatgca tcaaaatgat ggataggcag ggcaaaatca tttccgaaat actggagctt
     3361 gtcagcctga atgatgggag aatcgtaccc atagctgaac cgttggacat agggcgcacg
     3421 gttgccgagt tgctgcccga ttttcaaacc ttggcagagg caaacaacca gcggttcgtc
     3481 acagatattc cagccgggca aattgtcctg tccgatccga ggctgctcca aaaggcacta
     3541 tccaatgtca tattgaatgc agttcagaac acgccgcagg gaggcgaggt acggatatgg
     3601 agtgagcctg gtgctgaaaa atgccgcctt tttgttttga acatgggcgt tcacattgat
     3661 gatactgcgc ttccaaggct gttcacccca ttctatcgca ttgatcaggc gcaaagcaga
     3721 aaaagtgggc gaagcggttt aggacttgcc atcgtacaaa aaacgctgga tgccatgagc
     3781 ctccagtatg cgctggaaaa cacctcggat ggcgttttgt tctggctgga tttaccgctc
     3841 acatcaacat tgtaaatatt taaaacttaa atgattttga ccgacaggta taaccctgcc
     3901 ggtctttttt tgctgctaca ggaaaactac agactggcta cagggaaagt acagatgcgc
     3961 ttgccataat aacaatcgta ccagccacaa atcgtagttt tattgcaaag gaggcattcg
     4021 atcaaatgga aaaaagcaac tatcattcca atgcggatca tcacaaacgg catatgaaac
     4081 aatccgtgga aaagcgggct tttctatgtg cgttcattat ttcgttcaca gtctgcacgc
     4141 tgtttttggg gcggagactg gcttccgtat tggaggcaac acagataccg cccatccctg
     4201 caactcatac aggcagcagc actgacgtag tggagaattt ggaggaaaac gctcttgcca
     4261 ccgccaaaga acagggagat gaacaggaat ggagcctgat tttagtgaac aggcagaacc
     4321 ccatccccgc acagtacgat gtggagcttg agcaactatc aaatggtgag cggatagata
     4381 ttcggatttc tccctatctt caagatttgt ttgatgccgc aagaactgat ggagtttacc
     4441 cgattgtcgc atccggatac cgaacaacag aaaaacagca agaaattatg gatgaaaaaa
     4501 ttgccgaata taaggcgaaa ggctacacct ctgcacaggc taaagcggaa gcagaaactt
     4561 gggtggccgt gccgggaacg agcgagcatc agcttggtct tgctgtggat atcaatgcgg
     4621 acggaattca ttcaacaggc aacgaggttt atagatggct ggatgaaaac agctatcgtt
     4681 ttggttttat tcgtcgctac ccaccagaca agacagagat aaccggtgtg agcaacgagc
     4741 cgtggcatta ccgatatgtt ggcatcgaag ctgccacaga gatgtacaac caaggggttt
     4801 gccttgagga atatttaaaa ccagaaaaat gagaaaagga tgttatgtta tggacagaaa
     4861 aagattgaca cagcgtttcc cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg
     4921 cttttatgcg ggaatgagat ttgacggctg tcgctatgcg cagacgatag gggaaaaatc
     4981 gctttcccat ttgctctttg aaacggattg cgcattatac aaccacaata ccggatttga
     5041 catgatatac caggaaaaca aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg
     5101 cttattgata agaccggggg aacccttttc tttctggtgg ctggtacgcc atgcggacaa
     5161 agataccccc tataaagacg gccttacggt gaccaatggt aaactcacca ccatgtcggg
     5221 cggcggtatg tgccagatga gcaatttact attttggatg ttcctgcata cgccattgac
     5281 aattatccag cgcagaggtc acgaagtaaa ggagtttcca gagccaaaca gtgacgagat
     5341 taaaggggtg gatgcaacca tttcagaggg ctggattgat ttaaaagtgc gaaacgatac
     5401 cgactgcacc taccaaatat gggtgacctt agatgatgag aaaatcatcg gtcaggtgtc
     5461 cgccgacaaa gagccccaag cattatacaa aattacaaat ggcagtatcc agtatgtccg
     5521 tgaaagtggc gggatttatg aatatgccca ggttaaacgg atgcaagttg ccttaggtac
     5581 cggggaaata atagattgca agctgcttta tacaaacaaa tgcaaaatct gttatcccct
     5641 cccggaaagt gtggatattc aggaggagaa ccaatgagaa aaagtgtggg cattactgtt
     5701 tttggatgcg aacaggatga ggcaaatgct ttccgcgctt tatcgccgga ttttcatatt
     5761 atccctacgc tgattagcga tgcgatatcg gcagacaacg caaaattggc cgctggcaat
     5821 caatgcgtta gcgtaggcca taagtccgag gtttccgagg cgacaattct tgcgctgaga
     5881 aaggtcgggg taaaatacat ttctacccgc agcatcggct gcgatcacat tgatacgact
     5941 gccgccgaga gaatgggaat ctcggttggc acggttgcgt attcgccgga cagcgttgcg
     6001 gattatgctt tgatgctgat gctgatggcc atacggggtg caaaacccac catgcacgcc
     6061 gtggcgcaac aagatttcag attggatcgt atccggggga aagaactggg ggatatgact
     6121 gtgggagtta ttggaaccgg ccatatcggg caagcggtcg tcaaaaggct gcggggattt
     6181 ggatgccatg tgctggccta tgataacagc cgaaaaatgg atgcagatta tgtccagctt
     6241 gatgagcttc taaaaaacag cgatattgtt acgctccatg tgccgctttg tgcggatacc
     6301 cgccatctga tcggtcagaa gcaaattgga gagatgaagc aaggcgcatt tttaatcaac
     6361 accgggcgcg gagcgcttgt cgataccggg gcgctggtgg aggcgttgga aagcggaaag
     6421 ctgggtggtg cggcactgga tgtgttggag ggcgaggatc agtttgttta taccgattgc
     6481 tcgcagaaag tgcttgacca tccctttttg tcgcagcttt taaggatgcc gaatgtgatc
     6541 atcacacccc atacggcgta ctacactgag cgtgtgctgc aggatactac agaaaaaaca
     6601 atcaggaatt gtctcaattt tgaaaggagt ttacagcatg aatagaataa aagtcgcaat
     6661 catcttcggc ggttgctcgg aggaacatga tgtgtcggta aaatccgcaa tagaaattgc
     6721 tgcgaacatt gatacggaaa aattcgatcc gcactacatc ggaattacaa aaaacggtgt
     6781 atggaagcta tgcaagaagc catgtacgga atgggaagcc gacagtctcc ccgccatact
     6841 ctccccggat aggaaaacgc atgggctgct tgtcatgaaa gaaagcgaat acgaaacacg
     6901 gcgtattgat gtggctttcc cggttttgca tggcaaatgc ggggaggatg gtgcgataca
     6961 ggggctgttt gtattgtctg gtatccccta tgtgggctgt gatattcaaa gctccgcagc
     7021 ttgcatggac aaatcactgg cctacattct tacaaaaaat gcgggcatcg ccgttcccga
     7081 atttcaaatg attgataaag gtgacaagcc ggaggcgggt gcgcttacct accctgtctt
     7141 tgtgaagccg gcacggtcag gttcgtcctt tggcgtaacc aaagtaaacg gtacggaaga
     7201 acttaacgct gcgatagaag cggcaggaca atatgatgga aaaatcttaa ttgagcaagc
     7261 gatttcgggc tgtgaggtcg ggtgtgcggt catggggaac gaggatgatt tgattgtcgg
     7321 cgaagtggat caaatccggc tgagccacgg taccttccgc atccatcagg aaaacgagcc
     7381 ggaaaaaggc tcagaaaatg cgatgattac agttcccgca gacattccgg tcgaggaacg
     7441 aaatcgggtg caggaaacgg caaagaaagt atatcgggtg cttggatgca gagggcttgc
     7501 ccgtgttgat ctttttttgc aggaggatgg cggcatcgtt ctaaatgagg tcaataccct
     7561 gcccggtttt acatcgtaca gccgctaccc acgtatgatg gccgccgcag gaatcacgct
     7621 tcctgcactg attgacagcc tgattacatt ggcgttaaag aggtgataag tatggaaaat
     7681 ggttttttgt ttttagatga aatgttgcat ggcgtccgtt gggatgccaa gtacgccaca
     7741 tgggataact tcacaggaaa accggtggat gggtatgagg tgaatcgcat catcggcaca
     7801 aaggccgtgg cgtttgctct gcgcgaggca caaatccatg cggctgcgct tggctatggc
     7861 ttgcttttat gggatggata tcggccaaga actgcggtgg actgcttcct gcgttgggca
     7921 gcgcaaccgg aggacaatct cacaaaagaa aaattttacc ccaatataga gcgagccgag
     7981 ttgattacaa agggttatgt ggcctcacaa tccagccata gccgtggaag cgcaattgat
     8041 cttacgctct accacctgga tacaggggaa cttgtttcaa tgggaagtaa cttcgatttt
     8101 atggacgaac ggtcgcatca tacagcaaaa gggatagggg atgcagaggc acaaaatcga
     8161 agatgcttgc gtaaaatcat ggaaagcagc ggatttcagt cttatcgctt tgaatggtgg
     8221 cactataagt tgattgatga gccatacccc gatacctatt ttaattttgc cgtttcataa
     8281 tgaaagtatt ggttttgtat cagattttgg atttgataaa atctatgctg gtcatggaca
     8341 atcttttact ggaaagtggt taaagcatct tttgtaatac attgcaaatt atagtattca
     8401 atctgcccag cggcagaaaa gaaaaaaacc gctgttgggc agacctttcc cacgcccatt
     8461 ttgtattttg ctggcggcgg ttttgagaaa tcaaggccgc cgttcttttc tggcagcaaa
     8521 aggtgtggcg gcccatagga ggatgccgct atgctgtttt gctattgcca tgctctaatc
     8581 cacttccacc actcaaaaaa agccttgccc ctccatgaac tgaaccccaa aagttgaact
     8641 atttaatgga ctgt
//
LOCUS       DAZUMT010000278         7958 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.guided.124, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000278 DAZUMT010000000
VERSION     DAZUMT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.guided.124"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG8FM_003062"
     CDS             128..1564
                     /locus_tag="ACG8FM_003062"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5095622.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG8FM_003063"
     CDS             1753..4200
                     /locus_tag="ACG8FM_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS5095623.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8FM_003064"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8FM_003064"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS5095624.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG8FM_003065"
     CDS             5487..5693
                     /locus_tag="ACG8FM_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095625.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG8FM_003066"
     CDS             complement(5690..6793)
                     /locus_tag="ACG8FM_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5095626.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7345..7893
                     /gene="aac(6')"
                     /locus_tag="ACG8FM_003067"
     CDS             7345..7893
                     /gene="aac(6')"
                     /locus_tag="ACG8FM_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5095627.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2205 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taatttttta ttttataagt aagataaata atgaaagaat agagatactg
     7261 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
     7321 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
     7381 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
     7441 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
     7501 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
     7561 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
     7621 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
     7681 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
     7741 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
     7801 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
     7861 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
     7921 agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DAZUMT010000279         4809 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.guided.156, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000279 DAZUMT010000000
VERSION     DAZUMT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.guided.156"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..654
                     /locus_tag="ACG8FM_003068"
     CDS             19..654
                     /locus_tag="ACG8FM_003068"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5095628.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            761..1999
                     /gene="mef(H)"
                     /locus_tag="ACG8FM_003069"
                     /gene_synonym="mefH"
     CDS             761..1999
                     /gene="mef(H)"
                     /locus_tag="ACG8FM_003069"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS5095629.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            2226..2645
                     /locus_tag="ACG8FM_003070"
     CDS             2226..2645
                     /locus_tag="ACG8FM_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5095630.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2650..2889
                     /locus_tag="ACG8FM_003071"
     CDS             2650..2889
                     /locus_tag="ACG8FM_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095631.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(3114..4037)
                     /locus_tag="ACG8FM_003072"
     CDS             complement(3114..4037)
                     /locus_tag="ACG8FM_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS5095632.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4069..>4809)
                     /gene="map"
                     /locus_tag="ACG8FM_003073"
     CDS             complement(4069..>4809)
                     /gene="map"
                     /locus_tag="ACG8FM_003073"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS5095633.1"
                     /translation="EIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFVRNYIESHG
                     GIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGGISDSCWAY
                     VVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLAIVRDFIGH
                     GVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGWTAYTRDGG
                     LSCQYEHTLAITKDGPRILTSQGEEGTY"
BASE COUNT         1562 a          800 c          851 g         1596 t
ORIGIN      
        1 ttgtattgga ggtaagttat ggcacgaaat aaatatccag aagaaacagt aaaactcatt
       61 ttagatgttt ctacaaaaat gttttgtgaa aaaggatatg ataacacatc attgcaagat
      121 attatcaatg aaacaaaact ttctaaaggt gctatttatc atcatttcga ttcaaaggaa
      181 gatattttaa aagcaatttt tcaaagaatt ggaaatgaaa atgcagtaat atttgctaaa
      241 attagagata acgatagttt aaacggttta gaaaagttaa gagaaatatt taagacagcg
      301 gtattcaatt ctaaccaaag cgttttattg acagtttctc ctcaactatt aaacaatcct
      361 cgttttcttg ctatgcaaat agaacaaatg tatgaaattg tagtacctga atttgttgaa
      421 cccgttttag aacaaggtat caaggataag tctttggaga ttgaaaattc acatgaaatt
      481 gcagaagtta tcatggtatt aagtaatgta tggctcaacc cattagttga aatgacggat
      541 aaagagggta tggaaaaacg ttgcaataca ttcaatgccc ttttaaatgg aataggcata
      601 gacacattat tagacgaaga aacaatttct gcatttatac agttttgtaa ataactgtat
      661 ttgttatccc tatacgatag actgctctat aaaagagcag tttatttaaa ccattatata
      721 aaccgacggt ctgtttgatt aaataaaaag gagagatttt atgcaagcag aaaaaaaact
      781 ttttaatcgt gattttatac ttgttgttat aggacagata atttctttat ttggaaacgc
      841 tatcctacga tttgcattac cgtactattt gttaaaagaa actggttcat cagctttgtt
      901 tggtattgct acagcctgct cttttttacc tatggttatt ttgtcgctgg taggtggtat
      961 tattgctgac aggacaaata aaagaaacat tatggtcata ctagattttg taacagcaat
     1021 cataatttta gtattttatt tgtcattggg aaaaattcct atcatacctt tgtttattgt
     1081 tgctttgatg ttactttatg gtatttctgg tgcatatcaa ccagcggtac aagcaagtat
     1141 tcctctattg gtatcatcag ataaacttac cgtcggtaat gctataataa atcaagtaaa
     1201 tactttagca aactttatcg gaccagctat aagcggagta gtttttcaat tttatggtat
     1261 caatcccatt ttaattgtta gtattatatg ttttactttt tcagccatta tggaaatttt
     1321 tatccatatt ccacaccaaa aaagacctcg tgaagtaggt gtaattgcag ttgctaaaaa
     1381 cgatttaaaa gatagttata cctttttgaa aaaggagaaa cccattttta tatccgtaat
     1441 ctttttggtt tgcattttca atttggtttt aagtgctgtt ttaattgttg gtctgcctat
     1501 cttaatttct gaagttttaa aaatgccaga tacactatat ggtttttcac aatcagcatt
     1561 atcattaggt ggtttatgtg gtggtatttt aacagctctt gttgcttcaa agttaaaact
     1621 aaaaaacctt tataccttgt tacttatttg ttctgtttct gttttactta tgggaatttc
     1681 gctattttta gaattgcctg caatagtttc ctactgggta gttacagtga tgtgctttgt
     1741 ggctatgggt gtatctacta tgtttgttgt tcaaatttac actcttgttc agacacaaac
     1801 accaccgcaa ttagtaggaa agataatggc tacgctggta gcagttgcta tgtgcggtca
     1861 acctattgga caaatggttt acggtatttt attcgatgta tttatacaag aaacatgggt
     1921 tgttatgttt gtagcttcta ttgcttcaat catgatttca atatattcaa aaagaatttt
     1981 tcaaaagtta gagatataat acaaggggtt aaatagtatg ttaggctaaa aacaatatat
     2041 aaaagaaaat ttgaaatctg ctaaaaggat agtgttataa aagaaagagt aatattgcag
     2101 tatttaaggc tcgcacaaat atatgttttg tgtgggcttt tttcttacca tttttctgta
     2161 tttctaaagg gtataaacgg tattccgttt atatatttca gcattagcag aaagggggtg
     2221 agattatgag gacatcttct ttcgagcata tcgtcagatt acagttcaat tctttaatgc
     2281 tgattgtcat taagaacaaa gtgaaaagtc gaaacagaca gcttgcaaga cattctaaac
     2341 gtgaagtatt gttttgtgaa atagccgaaa cgaaacaagt tgaacgtgga acgattgata
     2401 cctgttttta tgattgtgta tcatttaaag tcttgcactt tacaatttat gtatctgatg
     2461 aaacacttgg tacagtttta catagactgt cagaaaaaca gcgtagtgcg attttattac
     2521 gttattttca aggaatgaac gacagagaaa tttcagaact gtaccatgta tcacgttctg
     2581 ctatttgcag tagaagaact agaggactaa ggaagttaaa aatgcttttg agcgaaagga
     2641 agtaaagaaa tgagtaaaga ttatggctat ccctcttttg aattgatttg caaagcgtca
     2701 agcggagatg aaatggcagt taaagagatt ttgaagtttt atgacggtta tatttctaaa
     2761 ttatgcttgc gtccttttta ccactctgaa agtggtaaaa tcattatgca ggttgatgaa
     2821 gaactaaaag gagaaattca tacagacatg atgaaagcaa tattgaaatt tgaaatcgga
     2881 gtgaagtaat caactgtata aaaaaatcgg gagatacaca gtttcgtgct atctccctta
     2941 gtagttttac ggtcatatca aggctcattc aatcgtggta aattcgtggt aagatgagtg
     3001 tttttctata cttcaaaatg cttgaatgta tagtgtttat gcggataatt gaaaattaga
     3061 tataaaattt caaataaatt actgatataa atttacaata agaactcttg tttctacttt
     3121 tttttatgag aaaatttagt tttcaattta ttgaaccatt ttcggcttgg atctcgacgt
     3181 tttttgattt gatgacccgt cacgtattct tgaacaactg cattgatgat tccgcccaaa
     3241 atgatgatcg tagcagcaaa attaagccaa agcatgagta caataaaact tcctatgatc
     3301 tgataaccgc tgatcctcgt tgcaaaatac ctagcataga tactaaacac ttgtgataac
     3361 agcatccagc cgacagtagc gaagatcgtg cccggaatga tcgtcttcaa cttcaactga
     3421 gcattaggga caattaaata aatcaagaac atgatgacaa ataaagctac caatgtgatt
     3481 ggccacttca aagtttggaa ctgatcgata aagttcacgg gtatttgtac gattggctga
     3541 atagcatcca aaatcagctg tcccaaccca agaacgatag ttaccccact aatagcaatc
     3601 atgaacaaga caatgacaat tagtgagaag aaacggacga tcaaaaaatt ttttctattt
     3661 tctacgccat acgctttatt catcgcaatt tgcaaagcat tgatactttg acttgcagac
     3721 cataaagccg ctaatgcaga aacggataac aagcctcctg aactttgtgt caaaaggtct
     3781 ttgattgcag gaccgataaa ctggtaaacg gtctcaggaa tcacttctcg taaataggtc
     3841 agtacagtct cttgatcgat gtggagaaac ggtaaaatat taccaaaagc aataatcaat
     3901 ggaaataaag acaagagtaa ataataagca acgacgacag aggtcgtact catttctgaa
     3961 tcagtgatgc gcttttgagt cgtctcaata aagcgcatca gattttcatt ttgttttatt
     4021 ttgttccaaa ctttcatgta cctctcccgt acctattgac tggatagatc aataagtgcc
     4081 ttcttctcct tgtgatgtca aaattcttgg accatcttta gtaatagcca atgtatgttc
     4141 atattggcaa gaaagtccgc cgtctcttgt atacgcagtc cagccgttag gatccatttt
     4201 cattttccaa gttccggtat taaccatagg ttcgatcgtg ataaccatgc cttctttcaa
     4261 ccgcaaccct tttccagctt ctccgtaatg aggaactgct ggttcttcat gaatcgtagg
     4321 accaacgcca tgaccaataa aatcacgaac gatcgctaga ttttcgcctt ctacataagt
     4381 ttggatagca tgaccgatgt caccaatccg attccctact tgtgcttgct caataccgat
     4441 gtacaaagct ttcttcgtta cttccatcaa tcggtcgatc tcaggtgtag attctccaac
     4501 tacataggcc caacaagaat cagaaatgcc gcctttcaaa tcgatacaca tatcgacttt
     4561 taccaaatca ccgtttttca aaggcttttt acgagggaat ccatgacaaa tttcatcatt
     4621 tatactgata caagtcgcat attcataccc ttcaaagcca atttgcgcag caattccgcc
     4681 atgactctcg atatagtttc tgacaaatac ctcaatatcc caacttgtga ttcctggttt
     4741 tataaaatca cgcagatttt tatgtacatc tgctaataaa gctccagatt ctgccatcat
     4801 ttcaatttc
//
LOCUS       DAZUMT010000280         4630 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.guided.158, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000280 DAZUMT010000000
VERSION     DAZUMT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.guided.158"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..617
                     /gene="dfrG"
                     /locus_tag="ACG8FM_003074"
     CDS             120..617
                     /gene="dfrG"
                     /locus_tag="ACG8FM_003074"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5095634.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(689..2641)
                     /locus_tag="ACG8FM_003075"
     CDS             complement(689..2641)
                     /locus_tag="ACG8FM_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5095635.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2648..2884)
                     /locus_tag="ACG8FM_003076"
     CDS             complement(2648..2884)
                     /locus_tag="ACG8FM_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095636.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3286..3576
                     /locus_tag="ACG8FM_003077"
     CDS             3286..3576
                     /locus_tag="ACG8FM_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095637.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(3848..4197)
                     /locus_tag="ACG8FM_003078"
                     /pseudo
     CDS             complement(3848..4197)
                     /locus_tag="ACG8FM_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4290..4592
                     /locus_tag="ACG8FM_003079"
                     /pseudo
     CDS             4290..4592
                     /locus_tag="ACG8FM_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1477 a          758 c          812 g         1583 t
ORIGIN      
        1 tgataatata acttatagaa aaaattgctt attcaactaa cgggcaggat aatgagaaaa
       61 ggttttttga tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa
      121 tgaaagtttc tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca
      181 ttccttggag gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga
      241 taatattagg taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata
      301 ttattctgac gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag
      361 aagatgtttt tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga
      421 tttataattt gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat
      481 tcgaaggaga tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc
      541 aaaaagggat aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa
      601 aaaacttatt gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag
      661 agcttgggat tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa
      721 attgtttatc tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt
      781 taaaagggcg acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact
      841 tcggattgaa gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc
      901 tttcaagaga tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat
      961 taacatttaa tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac
     1021 aatcaactga ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg
     1081 catatttgcg atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg
     1141 ttagtaattc agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc
     1201 tgatatctcc ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt
     1261 ttctgctttt cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt
     1321 ctttcgtgaa tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc
     1381 gccattcgtc ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag
     1441 gagcatcaac atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt
     1501 gaagataagc attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc
     1561 aatctaacct atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg
     1621 tatcttcatt taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca
     1681 catatcttga taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa
     1741 accccgtata tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca
     1801 tcttgtccgt attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat
     1861 atcgttctaa aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc
     1921 caactccaat tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct
     1981 tagcgaacat tggaagaatg gtgttctttt gttggtgata agtagtggtt tcttctcttt
     2041 ttggtagaac attagtgaac ttcttacacg ccttacattg atagcgttga gatttgcccc
     2101 tacttttccc ccttttgtaa aattgcttcg gctcattgaa tggggttgat tcttcttccg
     2161 agcagccttc tttatgaaat tgataatctg gctcaacatc ttggatgcta ttgatttcta
     2221 taaggcgttt aatttcttca acaacagacc aattagataa aggggttact gtacagtttt
     2281 gcgatacacc tctgccaata ggattgtcat tgcagtacat acctttatga cctttatctt
     2341 tggaactacc agtcatttta tatcgacttg gtttcccttt tacatctttg aatttgtgct
     2401 gttctttccc aaaattacaa caaaacggat tgatgcagta attatattga atgggatatt
     2461 cagaaccgtt ccatttaaaa gacgttggca agaacaacat tttgttatac cttgttgcaa
     2521 atcgttttgg agttaataaa tcatattgtt tagccctatc ttttaattct tcgtttgaaa
     2581 tcggaatttt aacatctaca atttcatctt ccttcgtggc taatctttta agcttggtca
     2641 ttgtctatca acccactcta tctttgatac ccattttttc aacaatccgc ttattacttc
     2701 gcttgttggc aatccattca ataaacttct tatcgtctaa aatgttcaat aagaattcat
     2761 ccactgcttc gctttcagta cgctctgcat attcagcgta ttgcttaact atcaccctta
     2821 cttgctctga tatttcccaa tccacagact tagcgttctt attttttggc tcaagaaatt
     2881 gcataggaaa atgcacccct ttttgcttag taaattgtaa ccattttaac acaccaattt
     2941 gcattcctac aataattatt tcacgttcgc atttttatat acattttaac ttacattttg
     3001 tggtgtaaat taacaacaaa cgttgattta acaataaaaa acgagtatca aattcagttg
     3061 caatttgata ctcatttagg tttacatttt cactttttgt actccaaaac tgaactactt
     3121 aaatctatac tgctgcctct tttttttagt acatgtcctt catcttcgca ggaagtgcca
     3181 atatgatttt aagaaaggta atagacaaaa agtcaaaaag ataaaacaga gtttcttctt
     3241 tacaaaggga atgtatgttc gtataatggt attgaggtga aggttatgga gaaaaaatat
     3301 ggaacagtag ataagatccg tactttgcaa gttagtgcca gaccgttgat ccgtttcagt
     3361 ttaaatgggg tcaattgttt gattgcttcc catagtttga acttcctagc tgaagtagac
     3421 gaagggatga agttagtcgt tacaggttat tataataata gaaagcaatt tgttgtgaga
     3481 tcctatcatg tgttagggaa acccaaaatt gtggtagaat atgagaaaag tctataccca
     3541 agaaaaaagg tccgacaaga tgaaagaata aactagaaaa caagcggctg agacaaaaat
     3601 gtcctagccg cttgttttgg aaagatggat gcagacgatg ggatttgaac ccacacgagc
     3661 atacgctcac aagaacctca atctatatat aactacatgt atttaagtat ttgtgatgaa
     3721 tcactaaact ttccaaactc atttgaggga tattgcttaa agattgaaca gatcaattcg
     3781 aagccctatt agtattcgct caatgggggg atcagaggcc ataaaaagag actagtcatt
     3841 actagtctca ggtagtggaa ttgtaggatt ttaaacgttc ctggagagta ccggtatgta
     3901 actcgaagag gtgattatca tagtcgtaaa agtaaattga agaagcctcg cctttcacac
     3961 gagaacgtcc tggttcgaca gttaaaccta acatttggat ttttgagaca aacgaatcta
     4021 tatcttgttc gtcaatttta aaagcaatat gattgtatgt ctttggcagt ttattcgacg
     4081 aattctgcat tactgcaatc cataggtcct taactaagaa aaatttttct ggatataatg
     4141 aaaatttttt tgttgactat cgtagatttc ttttgcatca aataaggtat ttaacaatta
     4201 agtatttaaa agataacttt taagttatct ttaagtaatg cttaaagcta tgttatactc
     4261 tattaggaaa tataagaagg agtataaaaa tgaacaaaca taatagaaaa ataacgaata
     4321 acaaagaact gatcttgtta ctaataggga tttggggtat ctttttactc tttaatatgt
     4381 atatgccgac tatgagagcg gatgacgtag tttatgcaag tagattagat gaacttggct
     4441 atttgggtgc ttcgatagaa cattataaga catggagttc aagaattatt atagaactat
     4501 tcttaatgtt cttttctaaa cactttatgc tatggaaatt attaaattct acgattatgt
     4561 taggaattgt agtattactt tgcaagtatg ttgagagtat aaaatatttt gtgtaaatga
     4621 aaaaatccat
//
LOCUS       DAZUMT010000281         4103 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.guided.167, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000281 DAZUMT010000000
VERSION     DAZUMT010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.guided.167"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..1531
                     /gene="msr(C)"
                     /locus_tag="ACG8FM_003080"
     CDS             53..1531
                     /gene="msr(C)"
                     /locus_tag="ACG8FM_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5095638.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1578..2024
                     /locus_tag="ACG8FM_003081"
     CDS             1578..2024
                     /locus_tag="ACG8FM_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5095639.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2223..2738)
                     /locus_tag="ACG8FM_003082"
     CDS             complement(2223..2738)
                     /locus_tag="ACG8FM_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5095640.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2855..4057
                     /locus_tag="ACG8FM_003083"
     CDS             2855..4057
                     /locus_tag="ACG8FM_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5095641.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1222 a          700 c          891 g         1290 t
ORIGIN      
        1 cttttttagt atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa
       61 tttagcagta aatataacaa atctacaagt cagctttggc aaccagctag aactatctat
      121 tgattctctt cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg
      181 taaatcgact ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca
      241 aacagagatc accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct
      301 aaatttggaa ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg
      361 gagcggaggg gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg
      421 gatgcttcta gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga
      481 agagcttcgt tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga
      541 tgagctagca tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa
      601 ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga
      661 gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc
      721 ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac cggatcggtt
      781 gtcttcctct aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt
      841 agagagaaga ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg
      901 ttttccagta ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt
      961 ccaattggaa agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg
     1021 taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat
     1081 ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca
     1141 aaagggttat caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga
     1201 gttttcagaa tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt
     1261 gacgaaaccg ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct
     1321 ttttactaag ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc
     1381 aacgatcgaa gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc
     1441 tcatgattct tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa
     1501 aataaaaaaa gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt
     1561 taaaggagtg gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga
     1621 tgtgtttttt gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc
     1681 atttatcaag tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg
     1741 tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa
     1801 gcaaggtcca aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta
     1861 tgtctttatt cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata
     1921 tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc
     1981 cgctttcaag aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt
     2041 gaaaaaaggc cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag
     2101 tgatccgaac gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt
     2161 gctcttttat tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc
     2221 ccttactcaa aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc
     2281 tgacaaagca taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct
     2341 agcgagaggt cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct
     2401 ctagtgaata gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt
     2461 gctagaaatt catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca
     2521 agagcacgtc cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc
     2581 aaaacggtca cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga
     2641 tgggcttccc aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat
     2701 aaaagcacgc ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta
     2761 ttttctttat tatatcacag taaagtttcc acgttttaca tgtgcatatt tacatgtaaa
     2821 ataaatcatg tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga
     2881 tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc
     2941 tacctgtatg atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc
     3001 tgcacagtta aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt
     3061 cactggtgtg gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac
     3121 attgatgaca ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa
     3181 tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc
     3241 tactgcttta gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat
     3301 gtatgaagca gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg
     3361 cagtggttca tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat
     3421 agccctagcg gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga
     3481 aggtgtcaaa gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata
     3541 taatttcgga ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga
     3601 attagaagtc ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt
     3661 cgttgcaccg gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt
     3721 cttattcatt atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc
     3781 gatcagcatt gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat
     3841 tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg
     3901 ctttttcggt ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga
     3961 aagctatgcg ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta
     4021 tcaccgtaat tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc
     4081 tgtcctgcat caagtcataa gaa
//
LOCUS       DAZUMT010000282         2772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5015
            SAMEA115707441-rid22576523.guided.190, whole genome shotgun
            sequence.
ACCESSION   DAZUMT010000282 DAZUMT010000000
VERSION     DAZUMT010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707441
            Sequence Read Archive: ERR13197170
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288023.1
            Genome Coverage       :: 164x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:10:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 36 of 82
            Pseudo Genes (incomplete)         :: 45 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707441-rid22576523.guided.190"
                     /isolate="VRE5015"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACG8FM_003084"
     CDS             111..194
                     /locus_tag="ACG8FM_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS5095642.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8FM_003085"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8FM_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS5095643.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="ACG8FM_003086"
     CDS             complement(1319..2182)
                     /locus_tag="ACG8FM_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5095644.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="ACG8FM_003087"
     CDS             complement(2184..2456)
                     /locus_tag="ACG8FM_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5095645.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="ACG8FM_003088"
     CDS             complement(2473..2682)
                     /locus_tag="ACG8FM_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS5095646.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
